CN117015603A - 使用基于转座子的技术与用于误差校正的独特分子标识符制备定向标签化测序文库的方法 - Google Patents

使用基于转座子的技术与用于误差校正的独特分子标识符制备定向标签化测序文库的方法 Download PDF

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Publication number
CN117015603A
CN117015603A CN202280022273.9A CN202280022273A CN117015603A CN 117015603 A CN117015603 A CN 117015603A CN 202280022273 A CN202280022273 A CN 202280022273A CN 117015603 A CN117015603 A CN 117015603A
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China
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sequence
transposon
umi
nucleic acid
target nucleic
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CN202280022273.9A
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Chinese (zh)
Inventor
苏珊·C·维瑞蒂
罗伯特·斯科特·库尔斯滕
尼尔·安东尼·戈麦利
安德鲁·B·肯尼迪
萨拉·E·舒尔扎伯格
安德鲁·斯莱特
艾玛·贝尔
塞巴斯蒂安·乔治·加布里埃尔·里科尔特
格雷斯·德桑蒂斯
菲奥娜·卡帕
庄涵宇
奥利弗·乔恩·米勒
杰森·理查德·贝特利
斯蒂芬·格罗斯
马茨·埃克斯特朗
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Illumina Cambridge Ltd
Illumina Inc
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Illumina Cambridge Ltd
Illumina Inc
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Publication of CN117015603A publication Critical patent/CN117015603A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202280022273.9A 2021-03-31 2022-03-29 使用基于转座子的技术与用于误差校正的独特分子标识符制备定向标签化测序文库的方法 Pending CN117015603A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163168802P 2021-03-31 2021-03-31
US63/168,802 2021-03-31
PCT/US2022/022379 WO2022212402A1 (en) 2021-03-31 2022-03-29 Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction

Publications (1)

Publication Number Publication Date
CN117015603A true CN117015603A (zh) 2023-11-07

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CN202280022273.9A Pending CN117015603A (zh) 2021-03-31 2022-03-29 使用基于转座子的技术与用于误差校正的独特分子标识符制备定向标签化测序文库的方法

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Country Link
US (1) US20240026348A1 (https=)
EP (1) EP4314283A1 (https=)
JP (1) JP2024511760A (https=)
KR (1) KR20230164668A (https=)
CN (1) CN117015603A (https=)
AU (1) AU2022249289A1 (https=)
BR (1) BR112023019945A2 (https=)
CA (1) CA3211172A1 (https=)
IL (1) IL307164A (https=)
MX (1) MX2023011218A (https=)
WO (1) WO2022212402A1 (https=)

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JP2026506068A (ja) * 2023-02-17 2026-02-20 ツイスト バイオサイエンス コーポレーション 正規化のための試薬および方法

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AU2022249289A1 (en) 2023-08-17
US20240026348A1 (en) 2024-01-25
WO2022212402A1 (en) 2022-10-06
CA3211172A1 (en) 2022-10-06
EP4314283A1 (en) 2024-02-07
IL307164A (en) 2023-11-01
BR112023019945A2 (pt) 2023-11-14
KR20230164668A (ko) 2023-12-04
JP2024511760A (ja) 2024-03-15
MX2023011218A (es) 2023-10-02

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