AU2022249289A1 - Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction - Google Patents

Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction Download PDF

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Publication number
AU2022249289A1
AU2022249289A1 AU2022249289A AU2022249289A AU2022249289A1 AU 2022249289 A1 AU2022249289 A1 AU 2022249289A1 AU 2022249289 A AU2022249289 A AU 2022249289A AU 2022249289 A AU2022249289 A AU 2022249289A AU 2022249289 A1 AU2022249289 A1 AU 2022249289A1
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sequence
double
umi
transposon
adapter
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Inventor
Emma BELL
Jason Richard Betley
Han-Yu Chuang
Grace Desantis
Mats Ekstrand
Niall Anthony Gormley
Stephen Gross
Fiona Kaper
Andrew B. Kennedy
Robert Scott Kuersten
Oliver Jon Miller
Sebastien Georg Gabriel RICOULT
Sarah E. SHULTZABERGER
Andrew Slatter
Susan C. Verity
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Illumina Cambridge Ltd
Illumina Inc
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Illumina Cambridge Ltd
Illumina Inc
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Publication of AU2022249289A1 publication Critical patent/AU2022249289A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2022249289A 2021-03-31 2022-03-29 Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction Pending AU2022249289A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163168802P 2021-03-31 2021-03-31
US63/168,802 2021-03-31
PCT/US2022/022379 WO2022212402A1 (en) 2021-03-31 2022-03-29 Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction

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AU2022249289A1 true AU2022249289A1 (en) 2023-08-17

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US (1) US20240026348A1 (https=)
EP (1) EP4314283A1 (https=)
JP (1) JP2024511760A (https=)
KR (1) KR20230164668A (https=)
CN (1) CN117015603A (https=)
AU (1) AU2022249289A1 (https=)
BR (1) BR112023019945A2 (https=)
CA (1) CA3211172A1 (https=)
IL (1) IL307164A (https=)
MX (1) MX2023011218A (https=)
WO (1) WO2022212402A1 (https=)

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JP2026506068A (ja) * 2023-02-17 2026-02-20 ツイスト バイオサイエンス コーポレーション 正規化のための試薬および方法

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US20240026348A1 (en) 2024-01-25
WO2022212402A1 (en) 2022-10-06
CA3211172A1 (en) 2022-10-06
EP4314283A1 (en) 2024-02-07
CN117015603A (zh) 2023-11-07
IL307164A (en) 2023-11-01
BR112023019945A2 (pt) 2023-11-14
KR20230164668A (ko) 2023-12-04
JP2024511760A (ja) 2024-03-15
MX2023011218A (es) 2023-10-02

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Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE INVENTOR TO READ VERITY, SUSAN C.; KUERSTEN, ROBERT SCOTT; GORMLEY, NIALL ANTHONY; KENNEDY, ANDREW B.; SCHULTZABERGER, SARAH E.; SLATTER, ANDREW; BELL, EMMA; RICOULT, SEBASTIEN GEORG GABRIEL; DESANTIS, GRACE; KAPER, FIONA; CHUANG, HAN-YU; MILLER, OLIVER JON; BETLEY, JASON RICHARD; GROSS, STEPHEN AND EKSTRAND, MATS