CN116970455A

CN116970455A - Health care wine with immunity improving function and preparation method thereof

Info

Publication number: CN116970455A
Application number: CN202311020983.0A
Authority: CN
Inventors: 王海平; 王德
Original assignee: Zhongxi Dongting Biotechnology Co ltd
Current assignee: Zhongxi Dongting Biotechnology Co ltd
Priority date: 2023-08-14
Filing date: 2023-08-14
Publication date: 2023-10-31

Abstract

The application discloses health care wine with an immunity improving function and a preparation method thereof, and belongs to the technical field of functional medicinal liquor. The application discloses health care wine with an immunity improving function, which comprises white spirit, deer penis, cordyceps militaris, oyster, deer blood, kudzu vine, honeysuckle and mint leaves. The application is used for brewing white spirit by a specific brewing method, and the white spirit is used for soaking the raw materials with the health care function, so that the content of methanol in the white spirit can be reduced, and the utilization rate of the raw materials and the leaching rate of active ingredients are improved. Meanwhile, the components are mutually cooperated to produce the effects of resisting fatigue, improving sleep, enhancing immunity and reducing blood fat; the adopted traditional Chinese medicines are harmless to human bodies, do not generate any side effect, and are safe and reliable.

Description

Health care wine with immunity improving function and preparation method thereof

Technical Field

The application belongs to the technical field of functional health care wine, and particularly relates to health care wine with an immunity improving function and a preparation method thereof.

Background

Along with the development of economy and high speed, the living standard of people is obviously improved, the modern life rhythm is accelerated, the pressure of people is increased gradually, and the long-term overload operation of the body of people brings serious harm to the health of people, such as immunity decline, hypomnesis, sexual desire decline, limb weakness and the like. Meanwhile, the health concept and the awareness of pursuing the quality of life are also enhanced, and the product requirements of people on the wine and food industry are greatly changed. The health wine is prepared by adding one or more Chinese medicinal materials into a certain production process for mixed fermentation or adding a proper amount of animal and plant extract into sake for blending, integrates the functions of health care, disease prevention and disease treatment, and is a dietary therapy health care method transmitted by the ethnic group of China.

In recent years, although there are many health fermented wines, the fermented wines have unsatisfactory or not strict effects of improving immunity due to insufficient raw material utilization and depending on blending of functional components due to the reasons of ingredients and production processes.

Disclosure of Invention

In order to overcome the technical defects, the application provides the health care wine with the function of improving the immunity and the preparation method thereof, and the production process of the base material white wine is regulated and controlled, so that the process can be completed from the source and in the brewing process, and the content of methanol in the health care wine is reduced. While at the same time. Can improve the immunity of human bodies after long-term administration, has the effects of improving sleep, reducing blood fat and resisting fatigue, is healthy, nutritional, safe and reliable, and is beneficial to health after long-term administration.

In a first aspect, the application provides a health wine with immunity improving function, comprising white spirit, penis et testis Cervi, cordyceps militaris, concha Ostreae, sanguis Cervi, radix Puerariae Lobatae, flos Lonicerae and folium Menthae;

the white spirit is prepared by the following steps:

aging the brewing grains, mixing the aged brewing grains with pectin esterase and cellulase, and performing enzymolysis;

centrifuging the enzymolysis product to obtain filter residue and supernatant, mixing the filter residue with water, rhizopus and malt saccharification liquid, and performing primary fermentation;

uniformly mixing the pre-fermented product with water and yeast, and performing main fermentation to obtain fermentation liquor;

mixing the fermentation liquor and the supernatant fluid and then distilling;

removing the distilled product from the turning wine and the tail wine, and aging to obtain the white wine.

Further, the material comprises the following raw materials in parts by weight:

450-600 parts of white spirit, 5-15 parts of penis cervi, 1-5 parts of cordyceps militaris, 1-6 parts of oyster, 1-6 parts of deer blood, 30-50 parts of kudzu vine, 10-30 parts of honeysuckle and 1-10 parts of mint leaves.

Further, the degree of the white spirit is 55-78 degrees.

Further, the grain curing process comprises the following steps: soaking the brewing grains in 80-90 deg.c water for 30-60 min and steaming at 100-110 deg.c for 20-30 min.

Further, the mass ratio of the brewing grain to the pectin esterase to the cellulase is 100-150:0.01-0.5:0.5-2, the enzymolysis time is 2-10 h, and the enzymolysis temperature is 35-50 ℃.

Further, the mass of the malt saccharification liquid is 1-3% of the mass of the filter residue, the mass of the rhizopus is 0.05-0.2% of the filter residue, the temperature of the primary fermentation is 28-32 ℃ and the time is 5-10 d.

Further, the mass ratio of the pre-fermentation product to the water to the yeast is 100-150:50:0.01-0.05, the temperature of the main fermentation is 25-28 ℃, and the time is 15-30 d.

Further, the mass ratio of the fermentation liquor to the supernatant is 1-30:1, and the distillation temperature is 70-85 ℃.

In a second aspect, the present application provides a method for preparing the health wine with immunity improving function according to any one of the first aspect, comprising the following steps:

pulverizing all the raw materials except the white spirit to obtain mixed powder;

and (3) carrying out intermittent microwave radiation treatment on the white spirit, and then putting the mixed powder into the white spirit for sealing and soaking.

Further, the batch microwave radiation treatment method comprises the following steps: and (3) carrying out microwave radiation on the white wine for 30-90 min at the temperature of 35-45 ℃ and carrying out intermittent operation at the frequency of 5s operation and 5s pause.

Compared with the prior art, the technical scheme provided by the embodiment of the application has at least the following advantages:

1. the health care wine with the immunity improving function provided by the application adopts a plurality of traditional Chinese medicine components with health care function, and the traditional Chinese medicine components are mutually compatible and coordinated to produce the effects of resisting fatigue, improving sleep, enhancing immunity and reducing blood fat. Meanwhile, the penis cervi, the cordyceps militaris, the oyster and the deer blood in the application are typical medicinal materials capable of improving the effects of tonifying qi and strengthening yang, and can realize the effects of strengthening the body resistance and consolidating the constitution, tonifying qi and nourishing blood, strengthening the spleen and soothing the liver, and removing stasis and detoxifying.

2. The health care wine with the immunity improving function provided by the application has the advantages that in the white wine brewing process, the fiber of grains is loosened through curing; the degradation of the fiber is accelerated by the treatment of the complex enzyme, so that more methanol is avoided; and through three stages of primary fermentation, main fermentation and distillation, the fermentation efficiency is improved, the fermentation period is shortened, and the utilization rate of raw materials is improved. In the process of preparing the health care wine, the white wine is subjected to intermittent microwave radiation treatment, so that the volatilization of methanol is accelerated, the quality and the taste of the health care wine can be improved, and the quick dissolution of the effective components of the traditional Chinese medicine is realized.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and together with the description, serve to explain the principles of the application.

In order to more clearly illustrate the embodiments of the application or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, and it will be obvious to a person skilled in the art that other drawings can be obtained from these drawings without inventive effort.

FIG. 1 is a process flow diagram of the preparation of a health wine with immunity enhancing function of the present application;

FIG. 2 is a report of detection of bacteria in the health wine prepared by the application;

FIG. 3 is a test report of food safety in the health wine prepared by the application.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

Unless otherwise specifically indicated, the various raw materials, reagents, instruments, equipment and the like used in the present application are commercially available or may be prepared by existing methods.

the white spirit is prepared by the following steps:

mixing the fermentation liquor and the supernatant fluid and then distilling;

In the application, penis and testis of deer are penis and testis of Cervus Nippon Temminck or Cervus Nippon Temminck of Cervidae. Sweet and salty in taste, warm in nature, enters liver, kidney and bladder meridians, has the effects of tonifying kidney essence, strengthening kidney yang and strengthening waist and knee, and is commonly used for treating kidney deficiency strain, soreness of waist and knee, deafness and tinnitus, impotence and spermatorrhea, and infertility due to cold womb. The penis et testis Cervi soaking liquor has effects of strengthening tendons and bones, can be used for treating various symptoms caused by yang deficiency of body such as soreness of waist and knees, weakness of limbs, and deficiency of kidney qi, and also has effects of promoting blood circulation, removing blood stasis, and dredging channels and collaterals. The penis et testis Cervi contains abundant proteins, fat and vitamins, and can be used for improving immunity and treating neurasthenia.

Cordyceps militaris is fungus of Cordyceps genus of Clavipitaceae, also called Cordyceps militaris, or Cordyceps militaris for short. Cordyceps militaris is a fungus for both food and medicine, the protein content of the Cordyceps militaris is up to 40.7%, and the contained amino acids necessary for human body are not only complete in variety and sufficient in quantity, but also in proper proportion. Cordyceps militaris also contains abundant vitamins, minerals and the like, and has good edible value. Cordyceps militaris stroma can be used as medicine, has mild taste, can nourish lung and kidney, stop bleeding and resolve phlegm, and can be used for treating pulmonary tuberculosis, senile weakness, anemia and weakness, etc. Cordyceps militaris contains rich cordycepic acid, cordycepin, ergosterol, etc., and has effects of expanding trachea, tranquilizing, resisting various bacteria, and lowering blood pressure.

Oyster commonly called oyster, etc., belongs to the phylum mollusca. Not only has fresh and delicious meat and rich nutrition, but also has unique health care function and medicinal value, and is a seafood with high nutritive value. Sex taste: pungent, warm and salty. Channel tropism: it is indicated for spleen, stomach, lung, liver, gall bladder and kidney meridians. The functions are as follows: has effects of warming spleen and stomach, lowering adverse qi, invigorating kidney, supporting yang, suppressing hyperactive liver, subsiding yang, tranquilizing, softening and resolving hard mass, astringing and inducing astringency. Dizziness, tinnitus, palpitation, insomnia, scrofula, goiter, mass, spontaneous perspiration, night sweat, spermatorrhea, metrorrhagia and metrostaxis, leukorrhagia, improving liver function, recovering fatigue, enhancing physical strength, having great close relation with activation of cell division regeneration red blood cells, promoting bile secretion, eliminating neutral fat accumulated in liver, and improving detoxification function of liver.

Deer blood is blood of Cervus Nippon Temminck or Cervus Nippon Temminck of Cervidae, and is obtained by taking blood during slaughtering deer, and air drying into purple brown sheet. The main functions are as follows: tonify deficiency and harmonize blood. Treating lumbago due to deficiency, palpitation, insomnia, and pulmonary flaccidity with hematemesis; in collapse, the belt is under. The deer blood soaking wine has effects of tonifying deficiency, regulating blood vessel, dispelling cold evil, relieving pain, relieving fatigue, resisting anoxia, invigorating kidney, supporting yang, regulating nerve and body fluid, and enhancing organism immunity. Has effects in invigorating asthenia, regulating blood circulation, dispelling cold evil, relieving pain, relieving fatigue, resisting anoxia, invigorating kidney, supporting yang, regulating nervous function and body fluid, and improving immunity, and can be used for treating yang deficiency cold, abdominal pain, kidney deficiency, sexual impotence, cold deficiency leukorrhagia, metrostaxis, anemia, lumbago, etc.

Gang Pu Gong Teng is pungent and sweet in flavor and cool in nature. Enter spleen and stomach. Has effects of relieving muscle and fever, promoting salivation, promoting eruption, and invigorating yang to arrest diarrhea. Is suitable for treating fever, headache, hard and thirsty, diabetes, measles, dysentery, diarrhea, hypertension, and cervical hyperbaric pain. The Gauga wine has effects of diminishing inflammation, relieving fever, resisting bacteria, regulating immunity, and relieving spasm and anticoagulation.

Jin Yin Hua is sweet and cold in nature. Has effects of clearing heat and detoxicating. Is mainly used for treating exogenous wind-heat or warm disease fever, heatstroke, toxic heat blood dysentery, carbuncle, furuncle, pharyngitis and various infectious diseases. The honeysuckle flower wine has the effects of dispelling wind and heat and clearing away heat and toxic materials, and can neutralize the drug properties of other medicinal materials in the application.

Peppermint leaf, folium Menthae, having effects of dispelling pathogenic wind and heat, clearing head and eyes, relieving sore throat, promoting eruption, soothing liver and promoting qi circulation. Exogenous wind-heat, headache, sore throat, food stagnation and bloating, aphtha, toothache, sore and scabies, urticaria, warm disease at the beginning, rubella itching, liver depression and qi stagnation, chest distress and hypochondriac pain. The dry folium Menthae soaking in alcohol has effects of dispersing pathogenic wind heat, refreshing brain, promoting eruption, promoting blood circulation, sweating, relieving pain, relieving itching, and dispelling pathogenic wind heat.

As shown in figures 2 and 3, the health care wine has low methanol content, does not contain mixed bacteria, and meets the food sanitary safety standard. The health care wine can repair human body functions, improve immunity, is rich in various active substances necessary for human body, can degrade harmful substances such as methanol, lead, benzoic acid and the like, and is beneficial to the growth of the human body as soon as possible.

Preferably, the adhesive comprises the following raw materials in parts by weight:

Preferably, the weight parts of the white spirit can be 450 parts, 460 parts, 470 parts, 480 parts, 490 parts, 500 parts, 510 parts, 520 parts, 530 parts, 540 parts, 550 parts, 560 parts, 570 parts, 580 parts, 590 parts or 600 parts, etc. The degree of the white spirit is 55-78 degrees, preferably 70-75 degrees.

Preferably, the parts by weight of the penis et testis Cervi may be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts or 15 parts, etc.

The weight parts of the cordyceps militaris can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts or 15 parts, etc.

The weight of oyster can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15.

The deer blood may be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, or the like by weight.

The weight parts of the kudzu can be 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts, etc.

The honeysuckle can be 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, or the like.

The weight part of the mint leaf can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts or the like.

Various embodiments of the application may exist in a range of forms; it should be understood that the description in a range format is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of the application; it is therefore to be understood that the range description has specifically disclosed all possible sub-ranges and individual values within that range. For example, it should be considered that a description of a range from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5, and 6, wherever applicable. In addition, whenever a numerical range is referred to herein, it is meant to include any reference number (fractional or integer) within the indicated range.

Further preferably, the composition comprises the following raw materials in parts by weight: 500 parts of white spirit, 10 parts of penis cervi, 10 parts of cordyceps militaris, 10 parts of oyster, 10 parts of deer blood, 30 parts of kudzu vine, 10 parts of honeysuckle and 3 parts of mint leaves.

Preferably, the white spirit is prepared by the following steps:

s1, soaking the brewing grains in water at 80-90 ℃ for 30-60 min, and then steaming at 100-110 ℃ for 20-30 min to obtain cured grains.

S2, mixing the cured grains with pectin esterase and cellulase for enzymolysis, wherein the mass ratio of the brewing grains to the pectin esterase to the cellulase is 100-150:0.01-0.5:0.5-2, the enzymolysis time is 2-10 h, and the enzymolysis temperature is 35-50 ℃.

S3, centrifuging the enzymolysis product at a speed of 1000-5000 rpm for 10-30 min to obtain filter residues and supernatant.

S4, mixing the filter residue with water, rhizopus and malt saccharification liquid, and performing primary fermentation, wherein the mass of the malt saccharification liquid is 1-3% of that of the filter residue, the mass of the rhizopus is 0.05-0.2% of that of the filter residue, and the primary fermentation temperature is 28-32 ℃ and the primary fermentation time is 5-10 d.

S5, uniformly mixing the pre-fermented product with water and yeast, and performing main fermentation to obtain fermentation liquor. The mass ratio of the pre-fermentation product to the water to the yeast is 100-150:50:0.01-0.05, the temperature of the main fermentation is 25-28 ℃, and the time is 15-30 d.

S6, mixing the fermentation liquor and the supernatant, and distilling, wherein the mass ratio of the fermentation liquor to the supernatant is 1-30:1, and the distilling temperature is 70-85 ℃.

S7, distilling, removing the head wine and the tail wine, and aging for 20-30 d to obtain the white spirit.

In a second aspect, the application provides a preparation method of a health wine with an immunity improving function, comprising the following steps:

pulverizing all the raw materials except the white spirit, and sieving with a 80-200 mesh sieve to obtain mixed powder;

and (3) carrying out intermittent microwave radiation treatment on the white spirit, and then putting the mixed powder into the white spirit, and sealing and soaking for 30-60 d.

Preferably, the batch microwave radiation treatment method comprises the following steps: and (3) carrying out microwave radiation on the white wine for 30-90 min at the temperature of 35-45 ℃ and carrying out intermittent operation at the frequency of 5s operation and 5s pause.

By increasing the temperature of the wine body, the microwave radiation treatment accelerates the mutual conversion of various components in the wine body and shortens the aging time of the white wine. The method can accelerate the chemical reaction of the wine body in a short time, generate richer aroma substances, quickly volatilize substances such as methanol in the wine body, promote the taste of the health wine, accelerate the dissolution of effective components in the traditional Chinese medicine powder, and accelerate the brewing speed of the health wine.

The principles and features of the present application are described below in connection with the following examples, which are set forth to illustrate, but are not to be construed as limiting the scope of the application. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

Example 1

The embodiment provides health care wine with the function of improving immunity, which comprises the following raw materials in parts by weight: 500g of white spirit, 10g of penis cervi, 10g of cordyceps militaris, 10g of oyster, 10g of deer blood, 30g of kudzu vine, 10g of honeysuckle and 3g of mint leaves.

Example 2

The embodiment provides health care wine with the function of improving immunity, which comprises the following raw materials in parts by weight: 600g of white spirit, 15g of penis cervi, 5g of cordyceps militaris, 6g of oyster, 5g of deer blood, 30g of kudzu vine, 10g of honeysuckle and 1g of mint leaf.

Example 3

The embodiment provides health care wine with the function of improving immunity, which comprises the following raw materials in parts by weight: 450g of white spirit, 5g of deer penis, 5g of cordyceps militaris, 1g of oyster, 2g of deer blood, 40g of kudzu vine, 20g of honeysuckle and 2g of mint leaves.

Example 4

As shown in fig. 1, the present embodiment provides a method for preparing the health wine with immunity improving function of embodiment 1, comprising the following steps:

s1, soaking the brewing grains in 80 ℃ water for 30min, and then steaming at 100 ℃ for 30min to obtain cured grains.

S2, mixing the cured grains with pectin esterase and cellulase for enzymolysis, wherein the mass ratio of the brewing grains to the pectin esterase to the cellulase is 150:0.01:0.5, the enzymolysis time is 10 hours, and the enzymolysis temperature is 35 ℃.

S3, centrifuging the enzymolysis product for 30min at 1000rpm to obtain filter residues and supernatant.

S4, mixing the filter residue with water, rhizopus and malt saccharification liquid, and performing primary fermentation, wherein the mass of the malt saccharification liquid is 3% of that of the filter residue, the mass of the rhizopus is 0.05% of that of the filter residue, and the primary fermentation temperature is 28 ℃ and the primary fermentation time is 5d.

S5, uniformly mixing the pre-fermented product with water and yeast, and performing main fermentation to obtain fermentation liquor. The mass ratio of the pre-fermented product to water to yeast is 150:50:0.01, the temperature of the main fermentation is 25 ℃, and the time is 30d.

S6, mixing the fermentation liquor and the supernatant, and distilling, wherein the mass ratio of the fermentation liquor to the supernatant is 5:1, and the temperature of the distillation is 75 ℃.

S7, distilling, removing the head wine and the tail wine, and aging for 20 days to obtain the white spirit.

S8, crushing all raw materials except the white spirit, and sieving the crushed raw materials with a 80-mesh sieve to obtain mixed powder.

S9, carrying out intermittent microwave radiation treatment on the white spirit, and then putting the mixed powder into the white spirit, and sealing and soaking for 30d.

The intermittent microwave radiation treatment method comprises the following steps: the wine was subjected to microwave irradiation at 45 ℃ for 45min and run intermittently at a frequency of 5s running for 5s pausing.

Example 5

The embodiment provides a preparation method of the health care wine with the function of improving immunity of the embodiment 2, which comprises the following steps:

s1, soaking the brewing grains in 80 ℃ water for 30min, and then steaming at 110 ℃ for 20min to obtain cured grains.

S2, mixing the cured grains with pectin esterase and cellulase for enzymolysis, wherein the mass ratio of the brewing grains to the pectin esterase to the cellulase is 150:0.01:0.5, the enzymolysis time is 2 hours, and the enzymolysis temperature is 35 ℃.

S3, centrifuging the enzymolysis product at 1000rpm for 10min to obtain filter residues and supernatant.

S4, mixing the filter residue with water, rhizopus and malt saccharification liquid, and performing primary fermentation, wherein the mass of the malt saccharification liquid is 1% of that of the filter residue, the mass of the rhizopus is 0.05% of that of the filter residue, and the primary fermentation temperature is 32 ℃ and the primary fermentation time is 5d.

S5, uniformly mixing the pre-fermented product with water and yeast, and performing main fermentation to obtain fermentation liquor. The mass ratio of the pre-fermented product to water to yeast is 100:50:0.01, the temperature of the main fermentation is 28 ℃, and the time is 15d.

S6, mixing the fermentation liquor and the supernatant, and distilling, wherein the mass ratio of the fermentation liquor to the supernatant is 30:1, and the temperature of the distillation is 70 ℃.

S8, crushing all raw materials except the white spirit, and sieving the crushed raw materials with a 200-mesh sieve to obtain mixed powder.

The intermittent microwave radiation treatment method comprises the following steps: the wine was subjected to microwave irradiation at 35 ℃ for 30min and run intermittently at a frequency of 5s running for 5s pausing.

Example 6

The embodiment provides a preparation method of the health care wine with the function of improving immunity of the embodiment 3, which comprises the following steps:

s1, soaking the brewing grains in 90 ℃ water for 60min, and then steaming at 110 ℃ for 30min to obtain cured grains.

S2, mixing the cured grains with pectin esterase and cellulase for enzymolysis, wherein the mass ratio of the brewing grains to the pectin esterase to the cellulase is 100:0.5:2, the enzymolysis time is 2 hours, and the enzymolysis temperature is 35 ℃.

S3, centrifuging the enzymolysis product at 5000rpm for 10min to obtain filter residues and supernatant.

S4, mixing the filter residue with water, rhizopus and malt saccharification liquid, and performing primary fermentation, wherein the mass of the malt saccharification liquid is 3% of that of the filter residue, the mass of the rhizopus is 0.2% of that of the filter residue, and the primary fermentation temperature is 30 ℃ and the primary fermentation time is 5d.

S5, uniformly mixing the pre-fermented product with water and yeast, and performing main fermentation to obtain fermentation liquor. The mass ratio of the pre-fermented product to the water to the yeast is 120:50:0.02, the temperature of the main fermentation is 28 ℃, and the time is 20d.

S6, mixing the fermentation liquor and the supernatant, and distilling, wherein the mass ratio of the fermentation liquor to the supernatant is 15:1, and the temperature of the distillation is 80 ℃.

S8, crushing all raw materials except the white spirit, and sieving the crushed raw materials with a 100-mesh sieve to obtain mixed powder.

S9, carrying out intermittent microwave radiation treatment on the white spirit, and then putting the mixed powder into the white spirit, and sealing and soaking for 45 days.

The intermittent microwave radiation treatment method comprises the following steps: the wine was subjected to microwave irradiation at 40 ℃ for 60min and run intermittently at a frequency of 5s running for 5s pausing.

Comparative example 1

The difference between this comparative example and example 4 is that the remaining raw materials of the traditional Chinese medicine are directly immersed in a commercially available white spirit having an alcohol content of 70 °.

Comparative example 2

The difference between this comparative example and example 4 is that in step S9, the mixed powder is directly poured into white spirit, and the white spirit is not treated by intermittent microwave radiation.

Comparative example 3

The present comparative example is different from example 4 in that the intermittent microwave irradiation treatment of step S9 is replaced with a water bath heating treatment, and the heating temperature and the heating time are unchanged.

Test example 1 toxicity test

20 healthy BALB/c mice (each half of female and male, weight of 18-22 g) are taken, and are subjected to adaptive feeding for 12 hours before the experiment, wherein the indoor temperature is 25+/-2 ℃. Mice were randomly divided into control and test groups of 10 each: and (5) normal feeding. Test group: the health wine prepared in the embodiment 1 of the application is used for feeding for 12 hours before stomach filling, the stomach filling amount is 2mL/10 g.bw, conventional feeding is carried out after stomach filling, and the stomach filling is carried out once a day for 1 week continuously. After the gastric lavage is finished, the mice of the control group and the test group are observed continuously for 14 days, the fur of the mice is smooth, and the appearance, the behavior, the diet, the activities, the secretion, the excrement and the like of the mice are not abnormal, so that the mice are dead.

Test example 2 immune function test

100 healthy BALB/c mice (each half of female and male, weight of 18-22 g) are taken, and are subjected to adaptive feeding for 12 hours before the experiment, wherein the indoor temperature is 25+/-2 ℃. Mice were randomly assigned to 5 groups of example 4, comparative examples 1-3 and blank, each group of 20. Wherein, the health care wine prepared in example 4 is used for lavage in the group of example 4, and the amount of the lavage is 0.2mL/10 g.bw; comparative examples 1-3 groups of health care wines prepared in comparative examples 1-3 with the same amount of stomach irrigation; the blank group was filled with the same amount of physiological saline. Each group of mice was gavaged 1 time per day for 30 days in succession. After the last gastric lavage, conA-induced mouse spleen lymphocyte transformation experiments were performed, and MTT assay was performed, with the test results shown in Table 1.

TABLE 1

	Number of mice	OD value
			Blank group	20	0.15±0.01
Example 4	20	0.34±0.09
			Comparative example 1	20	0.21±0.02
Comparative example 2	20	0.25±0.07
			Comparative example 3	20	0.23±0.03

Test example 3 clinical

In order to prove the health care effect of the health care wine provided by the application, the applicant carries out further treatment and clinical test on partial sub-health people who are easy to fatigue, easy to insomnia and need to improve immunity and people with higher blood fat content,

the method comprises the following steps:

selecting an object: 150 cases were selected, of which 80 men and 70 women had an age of 29 years, 54 years, and an average age of 41 years. Patients have different symptoms such as easy fatigue, easy insomnia, low immunity or higher blood lipid content. The above patients were equally divided into 3 groups of 50 persons each.

The treatment method comprises the following steps: the health care wine prepared in the examples 4-6 is sequentially used by 3 groups of patients, the dosage is 20-25 mL/time, 1 time a day, 7 days are a treatment course, and statistics are carried out after 4 treatment courses.

Curative effect judgment standard:

the effect is shown: the symptoms are relieved after the first use, and the symptoms are completely eliminated after the treatment course is finished.

The method is effective: the symptoms can be relieved after multiple times of use, and the symptoms can be completely eliminated after the treatment course is finished.

Invalidation: the symptoms are not obviously improved.

The experimental results are shown in table 2.

TABLE 2

	Has obvious effect	Effective and effective	Invalidation of	Total effective rate
					Example 4	36	13	1	98％
Example 5	30	17	3	94％
					Example 6	29	18	3	94％
Comparative example 1	15	25	10	80％
					Comparative example 2	20	24	6	88％
Comparative example 3	15	30	5	90％

The foregoing is only a specific embodiment of the application to enable those skilled in the art to understand or practice the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A health wine with immunity improving function is characterized by comprising white wine, penis et testis Cervi, cordyceps militaris, concha Ostreae, sanguis Cervi, radix Puerariae, flos Lonicerae and folium Menthae; the degree of the white spirit is 55-78 degrees.

2. The health wine with the immunity improving function according to claim 1, which is characterized by comprising the following raw materials in parts by weight:

3. The health wine with immunity enhancing function according to claim 1 or 2, wherein the white spirit is prepared by the following method:

mixing the fermentation liquor and the supernatant fluid and then distilling;

4. The health wine with immunity enhancing function as claimed in claim 3, wherein the grain ripening process is: soaking the brewing grains in 80-90 deg.c water for 30-60 min and steaming at 100-110 deg.c for 20-30 min.

5. The health wine with immunity improving function according to claim 3, wherein the mass ratio of the brewing grain to the pectin esterase to the cellulase is 100-150:0.01-0.5:0.5-2, the enzymolysis time is 2-10 h, and the enzymolysis temperature is 35-50 ℃.

6. The health wine with immunity enhancing function according to claim 3, wherein the mass of malt saccharification liquid is 1-3% of the mass of filter residue, the mass of rhizopus is 0.05-0.2% of the mass of filter residue, the temperature of the pre-fermentation is 28-32 ℃ and the time is 5-10 d.

7. The health wine with immunity enhancing function according to claim 3, wherein the mass ratio of the pre-fermented product, water and yeast is 100-150:50:0.01-0.05, the temperature of the main fermentation is 20-28 ℃, and the time is 15-30 d.

8. The health wine with immunity enhancing function as claimed in claim 3, wherein the mass ratio of the fermentation liquid to the supernatant is 1-30:1, and the distillation temperature is 70-85 ℃.

9. A method for preparing the health wine with the function of improving immunity according to any one of claims 1 to 8, which is characterized by comprising the following steps:

10. The method for preparing the health wine with the immunity improving function according to claim 9, wherein the intermittent microwave radiation treatment method is as follows: and (3) carrying out microwave radiation on the white wine for 30-90 min at the temperature of 35-45 ℃ and carrying out intermittent operation at the frequency of 5s operation and 5s pause.