CN116953266A - Microfluidic detection system, control method thereof and refrigerator - Google Patents
Microfluidic detection system, control method thereof and refrigerator Download PDFInfo
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- CN116953266A CN116953266A CN202210406710.9A CN202210406710A CN116953266A CN 116953266 A CN116953266 A CN 116953266A CN 202210406710 A CN202210406710 A CN 202210406710A CN 116953266 A CN116953266 A CN 116953266A
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- 238000001514 detection method Methods 0.000 title claims abstract description 214
- 238000000034 method Methods 0.000 title claims abstract description 67
- 239000000872 buffer Substances 0.000 claims abstract description 98
- 239000007853 buffer solution Substances 0.000 claims abstract description 66
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 238000003860 storage Methods 0.000 claims abstract description 27
- 238000005259 measurement Methods 0.000 claims abstract description 19
- 230000008859 change Effects 0.000 claims description 35
- 238000002835 absorbance Methods 0.000 claims description 26
- 239000000447 pesticide residue Substances 0.000 claims description 22
- 238000005303 weighing Methods 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims description 19
- 238000001802 infusion Methods 0.000 claims description 13
- 238000011161 development Methods 0.000 claims description 12
- 230000005764 inhibitory process Effects 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 230000015654 memory Effects 0.000 claims description 9
- 230000003213 activating effect Effects 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 239000000523 sample Substances 0.000 description 115
- 239000012488 sample solution Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000012916 chromogenic reagent Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
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- 230000002349 favourable effect Effects 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 239000000575 pesticide Substances 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
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- 238000011895 specific detection Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D11/00—Self-contained movable devices, e.g. domestic refrigerators
- F25D11/02—Self-contained movable devices, e.g. domestic refrigerators with cooling compartments at different temperatures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/025—Fruits or vegetables
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Abstract
The invention relates to a microfluidic detection system, a control method thereof and a refrigerator. The microfluidic detection system comprises a sample cup for containing a sample, a buffer storage tank for storing buffer for detection, and a buffer driving device for driving the buffer in the buffer storage tank to flow into the sample cup. The control method of the invention comprises the following steps: acquiring the weight of a sample in a sample cup; determining a target weight of the buffer solution required according to the weight of the sample in the sample cup; determining a target working time length of the buffer driving device according to the target weight of the required buffer; starting a buffer driving device; and controlling the start and stop of the buffer solution driving device according to the actual measurement working time after the buffer solution driving device is started and the actual measurement weight of the buffer solution in the sample cup. According to the invention, the starting and stopping of the buffer liquid driving device are comprehensively controlled according to the actual measurement working time after the buffer liquid driving device is started and the actual measurement weight of the buffer liquid in the sample cup, so that the buffer liquid amount injected into the sample cup can be controlled more accurately.
Description
Technical Field
The invention relates to a refrigeration technology, in particular to a microfluidic detection system, a control method thereof and a refrigerator.
Background
With the improvement of the living standard of people, pesticide residues, viruses, nutrient elements or other aspects of edible materials are generally required to be detected in daily life so as to qualitatively or quantitatively acquire the condition of the edible materials. For example, due to the problem of pesticide abuse, the daily purchased fruits and vegetables and agricultural and sideline products may have the problem of exceeding the pesticide residue content, and if the problem of exceeding the pesticide residue content of the foods cannot be timely found, the foods can cause great harm after being ingested by human bodies. For another example, breast feeding currently advocated is best feeding infants only if breast milk has normal nutritional value, however, the reduction of the content of nutrients in milk secreted by the infants and even the production of viruses may occur in the event of a sick, eaten, operated or otherwise by the lactating mother, thereby affecting the growth and health of the infants.
Among the numerous detection methods, the detection method using the microfluidic biological detection technology is relatively rapid, has a small volume, and is convenient to integrate on a refrigerator for household use. Microfluidic biological detection techniques require dilution or dissolution of a sample with the aid of a buffer to obtain a sample liquid, which is then reacted with a biological enzyme or other detection reagent. The buffer solution and the sample to be tested are mixed according to a certain proportion, and the accuracy of the detection result is directly affected by too much or too little buffer solution.
Disclosure of Invention
It is an object of a first aspect of the present invention to overcome at least one of the drawbacks of the prior art by providing a control method of a microfluidic detection system capable of accurately controlling the amount of buffer injected into a sample cup.
A further object of the first aspect of the present invention is to improve the detection efficiency and the accuracy of the detection results.
It is an object of a second aspect of the present invention to provide a microfluidic detection system capable of precisely controlling the amount of buffer injected into a sample cup.
An object of a third aspect of the present invention is to provide a refrigerator having the microfluidic detection system described above.
According to a first aspect of the present invention, there is provided a control method of a microfluidic detection system for qualitatively and/or quantitatively detecting a preset detection parameter of a sample, the microfluidic detection system including a sample cup for accommodating the sample, a buffer storage tank for storing a buffer for detection, and a buffer driving device for driving the buffer in the buffer storage tank to flow into the sample cup, the control method comprising:
acquiring the weight of a sample in the sample cup;
determining a target weight of buffer solution required according to the weight of the sample in the sample cup;
determining a target working time length of the buffer solution driving device according to the target weight of the required buffer solution;
starting the buffer driving device; and
and controlling the start and stop of the buffer solution driving device according to the actually measured working time after the buffer solution driving device is started and the actually measured weight of the buffer solution in the sample cup.
Optionally, the step of controlling the start and stop of the buffer solution driving device according to the actually measured working time after the buffer solution driving device is started and the actually measured weight of the buffer solution in the sample cup includes:
acquiring the actual measurement working time after the buffer solution driving device is started and the actual measurement weight of the buffer solution in the sample cup; and
stopping the buffer solution driving device when the actual measurement working time length of the buffer solution driving device reaches the target working time length or the actual measurement weight of the buffer solution in the sample cup reaches the preset weight; wherein the method comprises the steps of
The preset weight is equal to or slightly greater than the target weight.
Optionally, the ratio between the difference between the preset weight and the target weight is any value ranging from 0 to 0.1.
Optionally, the buffer solution storage tank is communicated with the sample cup through an infusion pipeline, and a pipeline heating device is arranged on the infusion pipeline; and is also provided with
Before starting the buffer driving device, the control method further comprises:
and starting the pipeline heating device to heat the infusion pipeline to a preset temperature value.
Optionally, after stopping the buffer driving device, the control method further includes:
suspending the sample cup so as to separate the sample cup from a weighing module of the microfluidic detection system;
and oscillating the sample cup to obtain a sample liquid.
Optionally, the microfluidic detection system further comprises a detection cell containing a chromogenic reagent, a reaction cell containing an enzyme reagent, and a light detection unit for detecting absorbance of the detection cell; and is also provided with
After obtaining the sample liquid, the control method further includes:
driving a preset amount of sample liquid in the sample cup to enter the reaction tank, and enabling the sample liquid entering the reaction tank to fully react with an enzyme reagent in the reaction tank;
driving the sample liquid fully reacted in the reaction tank to enter the detection tank;
activating a light source of the light detection unit;
respectively acquiring light intensity signals for representing the intensity of light transmitted through the detection cell when the color development reaction in the detection cell starts and after the color development reaction is completed;
calculating the light intensity change, wherein the light intensity change is the difference between a light intensity signal obtained after the completion of the color development reaction and a light intensity signal obtained at the beginning of the color development reaction; and
and obtaining a detection result of a preset detection parameter of the sample liquid according to the light intensity change.
Optionally, the step of acquiring light intensity signals representing the intensity of light transmitted through the detection cell at the beginning of the color reaction and after the completion of the color reaction, respectively, includes:
acquiring a light intensity signal when a color reaction starts after the light source is started for a first preset time period;
causing the sample liquid to creep back and forth in the detection pool for a plurality of times and then standing;
and obtaining a light intensity signal after the color development reaction is completed after the sample liquid is kept stand in the detection tank for a second preset time.
Optionally, before acquiring the weight of the sample in the sample cup, the control method further comprises: a wavelength initialization procedure, the wavelength initialization procedure comprising:
activating a light source of the light detection unit;
detecting a parameter value of a preset parameter related to the wavelength of light emitted by the light source, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting the duty ratio of a PWM control signal of the light source, wherein the target parameter value is set to be a preset wavelength value when the preset parameter of the light source is the target parameter value;
recording the duty ratio of the PWM control signal as a target duty ratio when the preset parameter of the light source is the target parameter value; and
turning off the light source; and is also provided with
And setting the duty ratio of the PWM control signal of the light source to the target duty ratio when the light source is started again in the detection process.
Optionally, the preset detection parameter is pesticide residue; and is also provided with
The step of obtaining the detection result of the preset detection parameters of the sample liquid according to the light intensity change comprises the following steps:
determining the absorbance of the detection pool according to the light intensity change;
calculating to obtain the pesticide residue inhibition rate of the sample liquid according to the determined absorbance of the detection pool; and
outputting pesticide residue detection results according to the calculated pesticide residue inhibition rate; wherein the method comprises the steps of
The step of determining the absorbance of the detection cell based on the change in light intensity comprises:
if the light intensity change is smaller than a preset minimum light intensity change, enabling the absorbance of the detection pool to be the preset minimum light intensity change;
if the light intensity change is larger than a preset maximum light intensity change, making the absorbance of the detection pool be the preset maximum light intensity change;
and if the light intensity change is between the preset minimum light intensity change and the preset maximum light intensity change, making the absorbance of the detection pool equal to the light intensity change.
According to an object of the second aspect of the present invention, there is also provided a microfluidic detection system for qualitatively and/or quantitatively detecting a preset detection parameter of a sample, the microfluidic detection system including a sample cup for accommodating the sample, a buffer storage tank for storing a buffer for detection, and a buffer driving device for driving the buffer in the buffer storage tank to flow into the sample cup, the microfluidic detection system further comprising:
the weighing module is used for weighing the sample cup to obtain the weight of the contained object in the sample cup; and
the control device comprises a processor and a memory, wherein a machine executable program is stored in the memory, and the machine executable program is used for realizing the control method according to any scheme when being executed by the processor.
According to an object of a third aspect of the present invention, there is also provided a refrigerator including:
a case defining a storage compartment therein for storing articles;
the door body is connected with the box body to open and/or close the storage compartment; and
the microfluidic detection system according to any one of the above embodiments is disposed on the case or the door.
The inventors have further realized that the power of the buffer drive for driving the flow of buffer is typically constant, and the amount of buffer flowing out per unit time via the buffer drive is constant, and thus the operating time of the buffer drive can also be used as a measure of the amount of buffer injected. Therefore, the control method of the invention firstly determines the target working time length of the buffer liquid driving device according to the target weight of the buffer liquid, and then comprehensively controls the start and stop of the buffer liquid driving device according to the actual measured working time length after the buffer liquid driving device is started and the actual measured weight of the buffer liquid in the sample cup, thereby being capable of controlling the buffer liquid amount injected into the sample cup more accurately.
Further, the inventors have recognized that detection reagents, particularly enzymes, typically have good activity or performance at a particular temperature to allow for adequate reaction with the sample fluid. Therefore, in order to sufficiently react the detection reagent with the sample liquid, the effect obtained by directly heating the detection cell in which the detection reagent is located to raise the temperature of the liquid in the detection cell to the optimal temperature is not ideal. The reason is that the volume of the microfluidic biochip is very small, the volume of the detection tank is smaller, the volume and the power of the heating device which can be adopted are very limited, and the direct heating of the detection tank is difficult to reach the reaction requirement under the environment of lower temperature, so that the detection result is inaccurate or the detection time is longer.
In order to solve the problem that the heating of the detection pool is severely limited, the invention does not seek meaningless breakthrough in the direction of directly heating the detection pool, but rather develops a way to control the temperature of the buffer solution for forming the sample solution in advance, namely, a pipeline heating device is arranged on the infusion pipeline between the buffer solution storage tank and the sample cup, before the buffer solution driving device is started, the pipeline heating device is started to heat the infusion pipeline to a preset temperature value, when the detection is carried out, the temperature of the sample solution formed by mixing the buffer solution with the temperature in a proper range in advance with the sample is proper, the sample solution with proper temperature can be fully reacted with the detection reagent directly, or can be fully reacted with the detection reagent after slight temperature adjustment, and the detection speed and the accuracy of the detection result are improved.
The above, as well as additional objectives, advantages, and features of the present invention will become apparent to those skilled in the art from the following detailed description of a specific embodiment of the present invention when read in conjunction with the accompanying drawings.
Drawings
Some specific embodiments of the invention will be described in detail hereinafter by way of example and not by way of limitation with reference to the accompanying drawings. The same reference numbers will be used throughout the drawings to refer to the same or like parts or portions. It will be appreciated by those skilled in the art that the drawings are not necessarily drawn to scale. In the accompanying drawings:
fig. 1 is a schematic block diagram of a microfluidic detection system according to one embodiment of the present invention;
fig. 2 is a partially exploded view of a microfluidic detection system according to one embodiment of the present invention;
fig. 3 is a schematic flow chart of a control method of a microfluidic detection system according to one embodiment of the present invention;
fig. 4 is a schematic flow chart of a control method of a microfluidic detection system according to another embodiment of the present invention;
fig. 5 is a schematic flow chart of a control method of a microfluidic detection system according to a further embodiment of the present invention;
fig. 6 is a schematic flow chart of a control method of a microfluidic detection system according to still another embodiment of the present invention;
fig. 7 is a schematic block diagram of a microfluidic detection system according to one embodiment of the present invention;
fig. 8 is a schematic structural view of a refrigerator according to an embodiment of the present invention.
Detailed Description
The invention firstly provides a control method of a microfluidic detection system, which is used for carrying out qualitative and/or quantitative detection on preset detection parameters of a sample. Specifically, the preset detection parameter may be, for example, an agricultural residue parameter for indicating whether the agricultural residue exceeds a standard and/or a specific value of the agricultural residue, a nutritional parameter for indicating whether the nutritional element meets a standard and/or a specific content of the nutritional element, a specific substance parameter for indicating whether a specific harmful substance (for example, a specific virus) exceeds a standard and/or a specific content, or the like.
Fig. 1 is a schematic structural view of a microfluidic detection system according to an embodiment of the present invention, and fig. 2 is a partially structural exploded view of the microfluidic detection system according to an embodiment of the present invention. Referring to fig. 1 to 2, the microfluidic detection system 10 of the present invention includes a sample cup 2 for accommodating a sample, a buffer storage tank 11 for storing a buffer for detection, and a buffer driving device 14 for driving the buffer in the buffer storage tank 11 to flow into the sample cup 2.
The inventors have realized that the accuracy of control of the amount of buffer injected is very high due to the very small weight of the sample to be tested. The microfluidic detection system proposed by the applicant of the present invention controls the injection amount of the buffer solution by weighing the sample cup, however, the injection amount of the buffer solution cannot be precisely controlled at all simply by using the way of weighing the sample cup due to the existence of a weighing error, the inertial influence of the flowing buffer solution, and the like.
The inventors have further appreciated that the power of the buffer drive 14 for driving the flow of buffer is generally constant and the amount of buffer that is driven out through the buffer drive 14 per unit time is constant, and thus the length of time the buffer drive 14 is operated can also be used as a measure of the amount of buffer injected. For this purpose, the control method of the present invention is proposed.
Fig. 3 is a schematic flow chart of a control method of a microfluidic detection system according to one embodiment of the present invention. In some embodiments, the control method of the present invention includes:
step S30, obtaining the weight of the sample in the sample cup 2;
step S41, determining the target weight of the needed buffer solution according to the weight of the sample in the sample cup 2;
step S42, determining the target working time length of the buffer driving device according to the target weight of the required buffer;
step S43, starting the buffer driving device 14; and
step S44, controlling the start and stop of the buffer driving device 14 according to the actual measurement working time after the buffer driving device 14 is started and the actual measurement weight of the buffer in the sample cup 2.
According to the control method, the target working time of the buffer solution driving device is determined according to the target weight of the buffer solution, and then the starting and stopping of the buffer solution driving device are comprehensively controlled according to the actual measured working time after the buffer solution driving device is started and the actual measured weight of the buffer solution in the sample cup, so that the buffer solution injected into the sample cup can be controlled more accurately.
In some embodiments, the step of controlling the start and stop of the buffer driving device 14 according to the actual measured working time period after the start of the buffer driving device 14 and the actual measured weight of the buffer in the sample cup 2 may specifically include:
acquiring the actual measurement working time after the buffer solution driving device 14 is started and the actual measurement weight of the buffer solution in the sample cup 2; and
stopping the buffer driving device 14 when the measured working time of the buffer driving device 14 reaches the target working time or the measured weight of the buffer in the sample cup 2 reaches the preset weight.
That is, stopping the buffer driving device 14 when either one of the measured operating time of the buffer driving device 14 and the measured weight of the buffer in the sample cup 2 reaches the requirement avoids the possibility of erroneous judgment caused by using only one parameter.
The inventors have realized that when the buffer flow speed driven by the buffer driving means 14 is low, the inertia of the buffer is small and almost negligible, and therefore the influence of the buffer inertia weighing module to acquire the weight of the sample cup 2 is small or even none, at this time, the above-mentioned preset weight can be set equal to the above-mentioned target weight, and the detection accuracy is high.
If the buffer solution driven by the buffer solution driving device 14 has a slightly high flowing speed, the buffer solution has a non-negligible inertia, so that the impact of the buffer solution on the sample cup 2 can cause the weight of the sample cup 2 measured by the weighing module to be slightly larger than the actual weight of the sample cup 2, at this time, the preset weight is set to be slightly larger than the target weight, that is, the preset weight is the sum of the target weight and the allowance corresponding to the flowing inertia of the buffer solution, various factors affecting the weighing of the sample cup 2 are considered, and the detection precision is high.
In particular, fig. 4 is a schematic flow chart of a control method of a microfluidic detection system according to another embodiment of the present invention. In other embodiments, the control method of the present invention includes:
step S30, obtaining the weight of the sample in the sample cup 2;
step S41, determining the target weight of the needed buffer solution according to the weight of the sample in the sample cup 2;
step S42, determining the target working time length of the buffer driving device according to the target weight of the required buffer;
step S43, starting the buffer driving device 14; and
step S441, obtaining the actual measurement working time length after the buffer solution driving device 14 is started and the actual measurement weight of the buffer solution in the sample cup 2;
step S442, judging whether the measured working time length of the buffer driving device 14 reaches the target working time length; if yes, go to step S444, if no, go to step S443;
step S443, judging whether the measured weight of the buffer solution in the cup 2 reaches the preset weight; if yes, go to step S444, if no, return to step S442;
in step S444, the buffer driving device 14 is stopped.
In some embodiments, the ratio between the difference between the predetermined weight and the target weight is any value ranging from 0 to 0.1. That is, the ratio of the margin of the preset weight exceeding the target weight to the target weight is any value between 0 and 0.1, which is more suitable for practical situations.
The inventors have realized that detection reagents, in particular enzymes, generally have a good activity or performance at a specific temperature in order to be able to react sufficiently with the sample fluid. Therefore, in order to sufficiently react the detection reagent with the sample liquid, the effect obtained by directly heating the detection cell in which the detection reagent is located to raise the temperature of the liquid in the detection cell to the optimal temperature is not ideal. The micro-fluidic chip has very small volume, the detection tank has smaller volume, the volume and the power of the heating device which can be adopted are very limited, and the direct heating of the detection tank is difficult to reach the reaction requirement under the environment of low temperature, so that the detection result is inaccurate or the detection time is long.
To this end, in some embodiments, the buffer reservoir 11 communicates with the sample cup 2 via an infusion line 15, and a line heating device 16 is provided on the infusion line 15. In these embodiments, the control method of the present invention further comprises, prior to activating the buffer drive 14:
the tubing heating device 16 is activated to heat the infusion tubing 15 to a preset temperature value.
In order to solve the problem that the heating of the detection pool is severely limited, the invention does not seek meaningless breakthrough in the direction of directly heating the detection pool, but rather develops a way to control the temperature of the buffer solution for forming the sample solution in advance, namely, a pipeline heating device is arranged on the infusion pipeline between the buffer solution storage tank and the sample cup, before the buffer solution driving device is started, the pipeline heating device is started to heat the infusion pipeline to a preset temperature value, when the detection is carried out, the temperature of the sample solution formed by mixing the buffer solution with the temperature in a proper range in advance with the sample is proper, the sample solution with proper temperature can be fully reacted with the detection reagent directly, or can be fully reacted with the detection reagent after slight temperature adjustment, and the detection speed and the accuracy of the detection result are improved.
Fig. 5 is a schematic flow chart of a control method of a microfluidic detection system according to still another embodiment of the present invention. In still other embodiments, after stopping the buffer driving device 14, the control method of the present invention further includes:
step S51, suspending the sample cup 2 so as to separate the sample cup 2 from the weighing module 13 of the microfluidic detection system 10;
in step S52, the sample cup 2 is oscillated to obtain a sample liquid.
According to the invention, after a corresponding amount of buffer solution is input into the sample cup 2, the sample liquid is suspended so as to separate from the weighing module 13 for acquiring weight, and then the sample cup 2 is oscillated so as to enable pesticide residues on a sample to be fully dissolved into the buffer solution, so that vibration of the sample cup 2 is not transmitted to the weighing module 13, the weighing precision of the weighing module 13 is not affected, high-precision measurement of the sample weight is ensured, and the accuracy of pesticide residue detection results is further improved.
In some embodiments, the microfluidic detection system 10 further comprises a detection cell 121 containing a chromogenic reagent, a reaction cell 122 containing an enzymatic reagent, and a light detection unit for absorbance detection of the detection cell 121. Specifically, the light detection unit includes a light source 161 for emitting light and a light intensity sensor 162 for detecting light intensity. In these embodiments, referring to a schematic flow chart of a control method of a microfluidic detection system according to still another embodiment of the present invention shown in fig. 6, after obtaining a sample liquid, the control method of the present invention further includes:
step S60, driving a preset amount of sample liquid in the sample cup 2 into the reaction tank 122, and enabling the sample liquid entering the reaction tank 122 to fully react with enzyme reagents in the reaction tank 122;
step S70, driving the sample liquid fully reacted in the reaction tank 122 to enter the detection tank 121;
step S80 of activating the light source 161 of the light detection unit;
step S91, respectively acquiring light intensity signals for representing the intensity of light transmitted through the detection cell 121 at the beginning of the color reaction in the detection cell 121 and after the completion of the color reaction;
step S92, calculating the light intensity change, wherein the light intensity change is the difference between a light intensity signal obtained after the completion of the color development reaction and a light intensity signal obtained at the beginning of the color development reaction; and
step S93, obtaining a detection result of a preset detection parameter of the sample liquid according to the light intensity change.
In some embodiments, the step S60 of acquiring the light intensity signals representing the intensity of the light transmitted through the detection cell 121 at the beginning of the color reaction and after the completion of the color reaction, respectively, may specifically include:
acquiring a light intensity signal at the beginning of a color reaction after the light source 161 is started for a first preset time period;
causing the sample liquid to creep back and forth in the detection cell 121 for a plurality of times and then standing;
the light intensity signal after the completion of the color reaction is obtained after the sample liquid is left in the detection cell 121 for a second preset period of time.
Specifically, the sample liquid may be driven to creep back and forth in the detection cell 121 by the sample liquid driving means. The sample fluid can be caused to react sufficiently with the chromogenic reagent by causing the sample fluid to creep back and forth within the detection cell 121. And, the sample liquid is kept stand for a period of time after creeping back and forth in the detection cell 121 for a plurality of times to acquire the light intensity signal, so that the influence of bubbles possibly existing in the detection cell 121 on the detection result is avoided.
In some embodiments, the control method of the present invention further comprises, prior to taking the weight of the sample in the sample cup 2: wavelength initialization procedure. Specifically, the wavelength initialization procedure may be performed after receiving the detection start instruction.
Further, the wavelength initialization process of the present invention specifically may include:
a light source 161 that activates the light detection unit;
detecting a parameter value of a preset parameter related to a wavelength of light emitted from the light source 161, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting a duty ratio of a PWM control signal of the light source 161, the target parameter value being set such that the wavelength of light emitted from the light source 161 is the preset wavelength value when the preset parameter of the light source 161 is the target parameter value;
recording the duty ratio of the PWM control signal as a target duty ratio when a preset parameter of the light source 161 is a target parameter value; and
the light source 161 is turned off.
Further, when the light source 161 is started again in the detection process, the duty ratio of the PWM control signal of the light source 161 is directly set to the target duty ratio. That is, in step S80, when the light source 161 is started up again after the sample liquid enters the detection cell 121, the duty ratio of the PWM control signal of the light source 161 is directly set to the target duty ratio.
The control method of the invention does not immediately start a specific detection flow after receiving the detection start instruction, but firstly executes the wavelength initialization flow of the light detection unit. In the wavelength initialization process, the duty ratio of the PWM control signal of the light source 161 is continuously adjusted by continuously detecting the parameter value of the preset parameter of the light source 161 related to the wavelength of the light emitted therefrom until the duty ratio of the PWM control signal of the light source 161 is found such that the parameter value of the preset parameter reaches the target parameter value (the wavelength of the light emitted from the corresponding light source 161 is the preset wavelength value), and is recorded as the target duty ratio. That is, the present invention first determines the target duty ratio of the PWM control signal of the light source 161 when the wavelength of the light emitted from the light source 161 is the preset wavelength value through the wavelength initialization procedure, thereby, in the detection procedure, the duty ratio of the PWM control signal of the light source 161 can be directly set to the target duty ratio, ensuring that the wavelength of the light emitted from the light source 161 is the optimal preset wavelength value, avoiding the adverse effect on the detection result caused by the wavelength fluctuation of the light emitted from the light source 161, and ensuring the accuracy of the detection result.
It can be appreciated that the predetermined wavelength value is the wavelength value most favorable for detection. For example, in the case of pesticide residue detection by the enzyme inhibition method, the wavelength of light emitted from the light source 161 is required to be optimal at 412nm, and the preset wavelength value is 412nm. Accordingly, when the light source 161 emits light having a wavelength of 412nm, the parameter value of the predetermined parameter is a target parameter value.
The inventors have realized that the light intensity is closely related to the wavelength of the light, and thus, in these embodiments, the predetermined parameter may be the light intensity detected by the light intensity sensor 162, and accordingly, the target parameter value is the target light intensity value.
The inventors have realized that the wavelength of the light emitted by the light source 161 is also related to the driving current of the light source 161. Therefore, in other embodiments, the predetermined parameter may be a driving current of the light source 161, and accordingly, the target parameter value is a target current value.
In some embodiments, the preset detection parameter detected by the microfluidic detection system 10 may be pesticide residues. In these embodiments, the step S93 of obtaining the detection result of the preset detection parameter of the sample liquid according to the light intensity variation may specifically include:
determining the absorbance of the detection cell 121 according to the light intensity variation;
calculating to obtain the pesticide residue inhibition rate of the sample liquid according to the determined absorbance of the detection pool 121; and
and outputting pesticide residue detection results according to the calculated pesticide residue inhibition rate.
Specifically, the step of determining the absorbance of the detection cell 121 according to the light intensity variation may specifically include:
if the light intensity variation is smaller than the preset minimum light intensity variation, making the absorbance of the detection cell 121 be the preset minimum light intensity variation;
if the light intensity variation is greater than the preset maximum light intensity variation, making the absorbance of the detection cell 121 be the preset maximum light intensity variation;
if the light intensity variation is between the preset minimum light intensity variation and the preset maximum light intensity variation, the absorbance of the detection cell 121 is made equal to the light intensity variation.
The microfluidic detection system 10 of the present invention directly sets the absorbance of the detection cell 121 to a preset maximum light intensity variation when the obtained light intensity variation is excessive (e.g., greater than the preset maximum light intensity variation), directly sets the absorbance of the detection cell 121 to a preset minimum light intensity variation when the obtained light intensity variation is excessively small (e.g., less than the preset minimum light intensity variation), and sets the absorbance of the detection cell 121 to an obtained light intensity variation when the obtained light intensity variation is appropriate. On the one hand, the problem that the calculated pesticide residue inhibition rate deviates from the theoretical range and causes using trouble to a user due to overlarge or overlarge absorbance of the detection pool 121 caused by the reasons that bubbles are generated in the detection pool 121, the sample liquid does not enter the detection pool 121, the chromogenic reagent is invalid and the like can be avoided; on the other hand, the accuracy of the calculated pesticide residue inhibition rate can be ensured when no fault occurs.
Further, if the light intensity variation is smaller than the preset minimum light intensity variation or the light intensity variation is larger than the preset maximum light intensity variation, the control method of the present invention further includes:
and sending out first fault prompt information which is used for prompting a user to detect possible faults and to test again.
Further, when the obtained light intensity variation is smaller than the preset minimum light intensity variation or larger than the preset maximum light intensity variation, there is a possibility that there are faults such as bubbles generated in the detection cell 121, the sample liquid not entering the detection cell 121, the failure of the color-developing reagent, and the like. Although the absorbance of the detection cell 121 is directly assigned as the preset minimum light intensity variation or the preset maximum light intensity variation at this time so that the calculated pesticide residue inhibition ratio is within the theoretical range, the calculated pesticide residue inhibition ratio is not accurate. Therefore, the invention also sends out the first fault prompt information at the moment to prompt the user that the fault possibly occurs in the detection and retests are needed, and the accuracy of the detection result is improved on the premise of ensuring that the use trouble is not brought to the user.
The present invention also provides a microfluidic detection system 10 for qualitatively and/or quantitatively detecting preset detection parameters of a sample, and the microfluidic detection system 10 includes a sample cup 2 for accommodating the sample, a buffer storage tank 11 for storing a buffer for detection, and a buffer driving device 14 for driving the buffer in the buffer storage tank 11 to flow into the sample cup 2.
Fig. 7 is a schematic block diagram of a microfluidic detection system according to one embodiment of the present invention. Referring to fig. 7, in particular, the microfluidic detection system 10 further comprises a weighing module 13 and a control device 18. The weighing module 13 is used for weighing the sample cup 2 to obtain the weight of the content in the sample cup 2. The control device 18 is connected to the weighing module 13 and comprises a processor 181 and a memory 182, the memory 182 storing a machine executable program 183, and the machine executable program 183 when executed by the processor 181 is adapted to carry out the control method described in any of the embodiments described above.
In particular, the processor 181 may be a central processing unit (central processing unit, CPU for short), or a digital processing unit or the like. The processor 181 transmits and receives data through a communication interface. The memory 182 is used for storing programs executed by the processor 181. Memory 182 is any medium that can be used to carry or store desired program code in the form of instructions or data structures and that can be accessed by a computer, and may be a combination of memories. The machine-executable program 183 described above may be downloaded from a computer-readable storage medium to a corresponding computing/processing device or downloaded to a computer or external memory device via a network (e.g., the internet, a local area network, a wide area network, and/or a wireless network).
The present invention also provides a refrigerator, and fig. 8 is a schematic structural view of a refrigerator according to an embodiment of the present invention. The refrigerator 1 of the present invention includes a cabinet 20 and a door 30. The case 20 defines a storage compartment therein for storing articles. The door 30 is connected to the case 20 to open and/or close the storage compartment. In particular, the refrigerator 1 further comprises the microfluidic detection system 10 described in any of the above embodiments, wherein the microfluidic detection system 10 is disposed on the case 20 or the door 30.
The refrigerator 1 provided by the invention is integrated with the microfluidic detection system 10, so that food material detection can be popularized to ordinary family life conveniently, and the convenience of use of users is improved.
Further, the microfluidic detection system 10 may be electrically connected to an electrical control device of the refrigerator 1 to provide power to the microfluidic detection system 1 via the electrical control device and/or to allow transmission of signals between the electrical control device and the microfluidic detection system 1.
Preferably, the microfluidic detection system 10 is preferably disposed on the door 30, which is convenient to operate, and does not occupy the original storage space in the case 20, and does not affect the storage capacity of the refrigerator 1 itself.
It should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected, can be indirectly connected through an intermediate medium, and can also be communicated with the inside of two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
By now it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been shown and described herein in detail, many other variations or modifications of the invention consistent with the principles of the invention may be directly ascertained or inferred from the present disclosure without departing from the spirit and scope of the invention. Accordingly, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.
Claims (11)
1. A control method of a microfluidic detection system for qualitatively and/or quantitatively detecting a preset detection parameter of a sample, and the microfluidic detection system includes a sample cup for accommodating the sample, a buffer storage tank for storing a buffer for detection, and a buffer driving device for driving the buffer in the buffer storage tank to flow into the sample cup, the control method comprising:
acquiring the weight of a sample in the sample cup;
determining a target weight of buffer solution required according to the weight of the sample in the sample cup;
determining a target working time length of the buffer solution driving device according to the target weight of the required buffer solution;
starting the buffer driving device; and
and controlling the start and stop of the buffer solution driving device according to the actually measured working time after the buffer solution driving device is started and the actually measured weight of the buffer solution in the sample cup.
2. The control method according to claim 1, wherein,
the step of controlling the start and stop of the buffer solution driving device according to the actually measured working time after the buffer solution driving device is started and the actually measured weight of the buffer solution in the sample cup comprises the following steps:
acquiring the actual measurement working time after the buffer solution driving device is started and the actual measurement weight of the buffer solution in the sample cup; and
stopping the buffer solution driving device when the actual measurement working time length of the buffer solution driving device reaches the target working time length or the actual measurement weight of the buffer solution in the sample cup reaches the preset weight; wherein the method comprises the steps of
The preset weight is equal to or slightly greater than the target weight.
3. The control method according to claim 2, wherein,
the ratio between the difference between the preset weight and the target weight is any value ranging from 0 to 0.1.
4. The control method according to claim 2, wherein the buffer storage tank is communicated with the sample cup through an infusion pipeline, and a pipeline heating device is arranged on the infusion pipeline; and is also provided with
Before starting the buffer driving device, the control method further comprises:
and starting the pipeline heating device to heat the infusion pipeline to a preset temperature value.
5. The control method according to claim 2, wherein,
after stopping the buffer driving device, the control method further includes:
suspending the sample cup so as to separate the sample cup from a weighing module of the microfluidic detection system;
and oscillating the sample cup to obtain a sample liquid.
6. The control method according to claim 5, wherein the microfluidic detection system further comprises a detection cell containing a color-developing reagent, a reaction cell containing an enzyme reagent, and a light detection unit for detecting absorbance of the detection cell; and is also provided with
After obtaining the sample liquid, the control method further includes:
driving a preset amount of sample liquid in the sample cup to enter the reaction tank, and enabling the sample liquid entering the reaction tank to fully react with an enzyme reagent in the reaction tank;
driving the sample liquid fully reacted in the reaction tank to enter the detection tank;
activating a light source of the light detection unit;
respectively acquiring light intensity signals for representing the intensity of light transmitted through the detection cell when the color development reaction in the detection cell starts and after the color development reaction is completed;
calculating the light intensity change, wherein the light intensity change is the difference between a light intensity signal obtained after the completion of the color development reaction and a light intensity signal obtained at the beginning of the color development reaction; and
and obtaining a detection result of a preset detection parameter of the sample liquid according to the light intensity change.
7. The control method according to claim 6, wherein,
the step of acquiring light intensity signals representing the intensity of light transmitted through the detection cell at the start of the color reaction in the detection cell and after the completion of the color reaction, respectively, includes:
acquiring a light intensity signal when a color reaction starts after the light source is started for a first preset time period;
causing the sample liquid to creep back and forth in the detection pool for a plurality of times and then standing;
and obtaining a light intensity signal after the color development reaction is completed after the sample liquid is kept stand in the detection tank for a second preset time.
8. The control method according to claim 6, wherein,
the control method further comprises, prior to taking the weight of the sample in the sample cup: a wavelength initialization procedure, the wavelength initialization procedure comprising:
activating a light source of the light detection unit;
detecting a parameter value of a preset parameter related to the wavelength of light emitted by the light source, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting the duty ratio of a PWM control signal of the light source, wherein the target parameter value is set to be a preset wavelength value when the preset parameter of the light source is the target parameter value;
recording the duty ratio of the PWM control signal as a target duty ratio when the preset parameter of the light source is the target parameter value; and
turning off the light source; and is also provided with
And setting the duty ratio of the PWM control signal of the light source to the target duty ratio when the light source is started again in the detection process.
9. The control method according to claim 6, wherein the preset detection parameter is pesticide residue; and is also provided with
The step of obtaining the detection result of the preset detection parameters of the sample liquid according to the light intensity change comprises the following steps:
determining the absorbance of the detection pool according to the light intensity change;
calculating to obtain the pesticide residue inhibition rate of the sample liquid according to the determined absorbance of the detection pool; and
outputting pesticide residue detection results according to the calculated pesticide residue inhibition rate; wherein the method comprises the steps of
The step of determining the absorbance of the detection cell based on the change in light intensity comprises:
if the light intensity change is smaller than a preset minimum light intensity change, enabling the absorbance of the detection pool to be the preset minimum light intensity change;
if the light intensity change is larger than a preset maximum light intensity change, making the absorbance of the detection pool be the preset maximum light intensity change;
and if the light intensity change is between the preset minimum light intensity change and the preset maximum light intensity change, making the absorbance of the detection pool equal to the light intensity change.
10. A microfluidic detection system for qualitatively and/or quantitatively detecting preset detection parameters of a sample, and the microfluidic detection system comprises a sample cup for accommodating the sample, a buffer storage tank for storing a buffer for detection, and a buffer driving device for driving the buffer in the buffer storage tank to flow into the sample cup, the microfluidic detection system further comprising:
the weighing module is used for weighing the sample cup to obtain the weight of the contained object in the sample cup; and
control device comprising a processor and a memory, said memory having stored therein a machine executable program, and said machine executable program when executed by said processor being adapted to carry out the control method according to any one of claims 1-9.
11. A refrigerator, comprising:
a case defining a storage compartment therein for storing articles;
the door body is connected with the box body to open and/or close the storage compartment; and
the microfluidic detection system of claim 10, disposed on the housing or the door.
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