WO2023185839A1

WO2023185839A1 - Microfluidic detection system and control method therefor, and refrigerator

Info

Publication number: WO2023185839A1
Application number: PCT/CN2023/084383
Authority: WO
Inventors: 赵斌堂; 费斌; 朱小兵
Original assignee: 青岛海尔电冰箱有限公司; 海尔智家股份有限公司
Priority date: 2022-04-02
Filing date: 2023-03-28
Publication date: 2023-10-05
Also published as: CN116928972A

Abstract

The present invention relates to a microfluidic detection system and a control method therefor, and a refrigerator. The microfluidic detection system comprises a microfluidic biochip in which a sample solution and a detection reagent react. The control method of the present invention comprises: when a first indication signal is acquired, sending warning information used for indicating that the temperature of a microfluidic biochip is excessively high, wherein the first indication signal is used for indicating that the temperature of the microfluidic biochip is excessively high; and when a second indication signal is acquired, heating the microfluidic biochip, wherein the second indication signal is used for indicating that the temperature of the microfluidic biochip is excessively low.

Description

Microfluidic detection system and control method thereof, refrigerator

Technical field

The invention relates to refrigeration and freezing technology, in particular to a microfluidic detection system and its control method, and a refrigerator.

Background technique

With the improvement of people's living standards, it is usually necessary to detect pesticide residues, viruses, nutritional elements or other aspects of some food ingredients in daily life to obtain the status of the food materials qualitatively or quantitatively. For example, due to the abuse of pesticides, the fruits, vegetables and agricultural and sideline products we buy daily may contain excessive pesticide residues. If the excessive pesticide residues in these foods are not discovered in time, it will cause great harm to the human body after ingestion. For another example, the breastfeeding currently advocated is the best feeding for the baby only if the breast milk has normal nutritional value. However, if the wet nurse is sick, takes medicine, has surgery or other circumstances, the nutrients in the milk secreted by the wet nurse may be compromised. The element content is reduced and even viruses are produced, thus affecting the growth, development and health of the baby.

When performing detection, it is usually necessary to resort to various detection reagents. The detection reagents, especially enzymes, only have good activity or performance at a specific temperature and can fully react with the sample liquid. If the temperature is too high or too low, it will affect the activity or performance of the detection reagent, thereby affecting the accuracy of the detection results.

Contents of the invention

An object of the first aspect of the present invention is to overcome at least one defect of the prior art and provide a control method for a microfluidic detection system that can avoid the temperature of a microfluidic biochip from being too high or too low.

A further object of the first aspect of the present invention is to improve the detection efficiency and the stability of temperature control of the microfluidic biochip.

The second object of the present invention is to provide a microfluidic detection system that can prevent the temperature of the microfluidic biochip from being too high or too low.

The third aspect of the present invention aims to provide a refrigerator with the above-mentioned microfluidic detection system.

According to a first aspect of the present invention, the present invention provides a control method for a microfluidic detection system, which is used to conduct qualitative and/or quantitative detection of preset detection parameters of a sample, and the microfluidic detection system The fluidic detection system includes a microfluidic biochip for allowing sample liquid and detection reagents to react within it, and the control method includes:

When the first indication signal is obtained, a prompt for indicating that the temperature of the microfluidic biochip is too high is issued. Wake up information, the first indication signal is used to indicate that the temperature of the microfluidic biochip is too high; and

When a second indication signal is obtained, the microfluidic biochip is heated, and the second indication signal is used to indicate that the temperature of the microfluidic biochip is too low.

Optionally, the first indication signal and the second indication signal are generated in the following manner:

Obtain the temperature of the microfluidic biochip;

The first indication signal is generated when the temperature of the microfluidic biochip is higher than the maximum endpoint value of the first preset temperature range;

The second indication signal is generated when the temperature of the microfluidic biochip is lower than the minimum endpoint value of the first preset temperature range.

Optionally, the microfluidic detection system further includes a heating device for heating the microfluidic biochip; and

The step of heating the microfluidic biochip includes:

Determine the target duty cycle after the heating device is activated according to the temperature of the microfluidic biochip; and

The operation of the heating device is controlled according to the target duty cycle.

Optionally, when the temperature of the microfluidic biochip is less than the first preset temperature value, the target duty cycle of the heating device is the first preset duty cycle;

When the temperature of the microfluidic biochip is greater than or equal to the first preset temperature value and less than or equal to the second preset temperature value, the target duty cycle of the heating device is the second preset duty cycle;

When the temperature of the microfluidic biochip is greater than the second preset temperature value, the target duty cycle of the heating device is the third preset duty cycle; wherein

The first preset temperature value is less than the second preset temperature value, the second preset temperature value is less than the minimum endpoint value of the first preset temperature range, the first preset duty cycle, The second preset duty cycle and the third preset duty cycle decrease in sequence.

Optionally, after heating the microfluidic biochip, the control method further includes:

Continue to obtain the temperature of the microfluidic biochip;

Determine whether the obtained temperature of the microfluidic biochip is greater than the maximum endpoint value of the second preset temperature range; if so, stop the heating device; if not, continue to heat the microfluidic biochip ;

Obtain the temperature of the microfluidic biochip again;

Determine whether the temperature of the microfluidic biochip obtained again is less than the minimum endpoint value of the second preset temperature range; if so, restart the heating device to reheat the microfluidic biochip. ; If not, maintain the stopped state of the heating device.

Optionally, the step of continuing to heat the microfluidic biochip includes:

Control the operation of the heating device according to a fourth preset duty cycle;

The step of restarting the heating device to reheat the microfluidic biochip includes:

Start the heating device and control the operation of the heating device according to the fourth preset duty cycle; wherein

The fourth preset duty cycle is less than or equal to the third preset duty cycle.

Optionally, the maximum endpoint value of the first preset temperature range is the sum of the preset temperature threshold and the first temperature difference value;

The minimum endpoint value of the first preset temperature range is the difference between the preset temperature threshold and the first temperature difference value;

The maximum endpoint value of the second preset temperature range is the sum of the preset temperature threshold and the second temperature difference value;

The minimum endpoint value of the second preset temperature range is the difference between the preset temperature threshold and the second temperature difference value; and

The first temperature difference value is greater than the second temperature difference value.

Optionally, before obtaining the first indication signal or the second indication signal, the control method further includes:

Receive a chip installation signal indicating that the microfluidic biochip has been installed.

According to a second aspect of the present invention, the present invention also provides a microfluidic detection system for qualitative and/or quantitative detection of preset detection parameters of samples, and the microfluidic detection system includes:

Microfluidic biochips are used for sample fluids and detection reagents to react within them;

Prompt device for issuing reminder messages;

A heating device for heating the microfluidic biochip; and

The control device includes a processor and a memory. A machine executable program is stored in the memory, and when the machine executable program is executed by the processor, it is used to implement the control method according to any of the above solutions.

According to a third aspect of the present invention, the present invention also provides a refrigerator, including:

A box defining a storage compartment for storing items;

A door body connected to the box body to open and/or close the storage compartment; and

The microfluidic detection system according to any of the above embodiments is provided on the box or the door.

The control method of the microfluidic detection system of the present invention not only heats the microfluidic biochip when the second indication signal indicating that the temperature of the microfluidic biochip is too low is obtained, but also obtains the second indication signal indicating that the temperature of the microfluidic biochip is too low. When the temperature of the microfluidic biochip is too high, the first indication signal is sent out in time to facilitate the user to take action. Using corresponding measures to cool down the microfluidic biochip not only avoids the adverse effects of the microfluidic biochip's temperature being too low on the test results, but also avoids the adverse effects of the microfluidic biochip's temperature being too high on the test results. , ensuring that the microfluidic detection system can obtain more accurate detection results in various usage environments.

Furthermore, when the temperature of the microfluidic biochip is low, the control method of the present invention also selects different target duty cycles of the heating device according to the temperature of the microfluidic biochip. On the one hand, when the temperature of the microfluidic biochip is When the temperature is too low, a larger target duty cycle can be used to increase the heating speed, thereby shortening the time consumed by the entire detection and improving detection efficiency; on the other hand, when the temperature of the microfluidic biochip is slightly lower , a smaller target duty cycle can be used to avoid excessive increase in the temperature of the microfluidic biochip caused by excessive heating speed, thereby avoiding large fluctuations in the temperature of the microfluidic biochip and improving the efficiency of the microfluidic biochip. Stability of biochip temperature control.

From the following detailed description of specific embodiments of the present invention in conjunction with the accompanying drawings, those skilled in the art will further understand the above and other objects, advantages and features of the present invention.

Description of drawings

Some specific embodiments of the invention will be described in detail below by way of illustration and not limitation with reference to the accompanying drawings. The same reference numbers in the drawings identify the same or similar parts or portions. Those skilled in the art will appreciate that these drawings are not necessarily drawn to scale. In the attached picture:

Figure 1 is a schematic flow chart of a control method of a microfluidic detection system according to one embodiment of the present invention;

Figure 2 is a schematic flow chart of a control method of a microfluidic detection system according to another embodiment of the present invention;

Figure 3 is a schematic flow chart of a control method of a microfluidic detection system according to another embodiment of the present invention;

Figure 4 is a schematic flow chart of a control method of a microfluidic detection system according to yet another embodiment of the present invention;

Figure 5 is a schematic flow chart of a control method of a microfluidic detection system according to yet another embodiment of the present invention;

Figure 6 is a schematic structural block diagram of a microfluidic detection system according to one embodiment of the present invention;

Figure 7 is a schematic structural diagram of a microfluidic detection system according to one embodiment of the present invention;

Figure 8 is a schematic structural diagram of a refrigerator according to an embodiment of the present invention.

Detailed ways

The present invention first provides a control method for a microfluidic detection system. The microfluidic detection system of the present invention is used to conduct qualitative and/or quantitative detection of preset detection parameters of samples. Specifically, the preset detection parameters can be, for example, Pesticide residue parameters are used to indicate whether the pesticide residue amount exceeds the standard and/or the specific value of the pesticide residue amount; nutritional parameters are used to indicate whether the nutrient element reaches the standard and/or the specific content of the nutrient element; used to indicate specific harmful substances (such as specific virus) exceeds the standard and/or specific substance parameters of specific content, etc.

Further, the microfluidic detection system may include a microfluidic biochip for reacting sample liquid and detection reagents therein.

The inventor realized that in the prior art, the microfluidic biochip usually only pays attention to the need to heat the microfluidic biochip when the temperature is too low, ignoring that in some special application environments, the microfluidic biochip may also have a temperature that is too high. question. Moreover, the temperature of the microfluidic biochip is too high or too low relative to the optimal reaction temperature of the detection reagent used. For example, if the optimal reaction temperature of the detection reagent is 20°C, any time the temperature of the microfluidic biochip exceeds 25°C is considered to be too high. However, in summer, microfluidic biochips placed in a room temperature environment will The temperature of the chip can easily reach 25°C. Therefore, whether the temperature of the microfluidic biochip is too high is also a matter of concern.

Based on this, the present invention proposes a control method for a microfluidic detection system. Figure 1 is a schematic flow chart of a control method for a microfluidic detection system according to an embodiment of the present invention. Referring to Figure 1, the control method of the present invention includes:

Step S10, determine whether the first indication signal is obtained; the first indication signal is used to indicate that the temperature of the microfluidic biochip is too high; if yes, go to step S20; if not, go to step S30;

Step S20, sending a reminder message indicating that the temperature of the microfluidic biochip is too high;

Step S30, determine whether a second indication signal is obtained; the second indication signal is used to indicate that the temperature of the microfluidic biochip is too low; and

Step S40, heat the microfluidic biochip.

That is to say, when the temperature of the microfluidic biochip is too high, the user is prompted; when the temperature of the microfluidic biochip is too low, the microfluidic biochip is heated. Specifically, the reminder information can be, for example, voice information containing specific semantics, simple sound information such as beeps, etc., or light information, such as emitting light of a specific color, flashing light, etc. Of course, the reminder information can also be other means of expressing to the user that the temperature of the microfluidic biochip is too high.

It can also be understood that the embodiment shown in Figure 1 is only one embodiment of the present invention. In essence, in order to achieve the purpose of the present invention, there is no strict sequence limit on the above steps S10 and S30, that is, they can be executed first Step S10 and then step S30 may be executed, or step S30 may be executed first and then step S10.

The control method of the microfluidic detection system of the present invention not only heats the microfluidic biochip when the second indication signal indicating that the temperature of the microfluidic biochip is too low is obtained, but also obtains the second indication signal indicating that the temperature of the microfluidic biochip is too low. When the first indication signal that the temperature of the microfluidic biochip is too high is detected, a reminder message is sent out in time, so that the user can take corresponding measures to cool down the microfluidic biochip, which not only avoids the detection of excessively low temperature of the microfluidic biochip. It also avoids the adverse effects of excessive temperature of the microfluidic biochip on the detection results, ensuring that the microfluidic detection system can obtain more accurate detection results in various usage environments.

In some embodiments, the first indication signal and the second indication signal may be generated by:

Obtain the temperature of the microfluidic biochip;

Generating a first indication signal when the temperature of the microfluidic biochip is higher than the maximum endpoint value of the first preset temperature range;

In other words, the temperature of the microfluidic biochip can be directly obtained, and based on the obtained temperature value, it can be determined whether the temperature of the microfluidic biochip is too high or too low. When the obtained temperature value is higher than the maximum endpoint value of the first preset temperature range, it means that the temperature of the microfluidic biochip is too high; when the obtained temperature value is lower than the minimum endpoint value of the first preset temperature range , indicating that the temperature of the microfluidic biochip is too low.

Specifically, FIG. 2 is a schematic flow chart of a control method of a microfluidic detection system according to another embodiment of the present invention. In some embodiments, the control method of the present invention includes:

Step S11, obtain the temperature of the microfluidic biochip;

Step S12, determine whether the temperature of the microfluidic biochip is higher than the maximum endpoint value of the first preset temperature range; if so, go to step S20; if not, go to step S31;

Step S31, determine whether the temperature of the microfluidic biochip is lower than the minimum endpoint value of the first preset temperature range; if so, go to step S40; if not, return to step S11; and

Step S40, heat the microfluidic biochip.

That is to say, when the temperature of the buffer solution is within the first preset temperature range, it means that the temperature of the microfluidic biochip is appropriate, and there is no need to cool it or heat it.

In some alternative embodiments, the first indication signal and the second indication signal can also be obtained indirectly through other indirect methods, such as through the ambient temperature. The first indication signal is generated when the ambient temperature is too high, and the first indication signal is generated when the ambient temperature is too low. Generate a second indication signal.

In some embodiments, the microfluidic detection system further includes a heating device for heating the microfluidic biochip. The inventor realized that the existing testing equipment is relatively large, and accordingly, the sample testing area is also relatively large. Temperature control is easier. The microfluidic detection system is suitable for integration on refrigerators for home use because of its fast detection speed and small size. For smaller microfluidic detection systems, the size of the microfluidic biochip used is even smaller. Using ordinary heating control schemes, it is impossible to control its temperature stably, which will lead to serious temperature fluctuations and easily affect the accuracy of test results.

To this end, in some embodiments, referring to the schematic flow chart of the control method of the microfluidic detection system according to another embodiment of the present invention shown in Figure 3, the step S40 of heating the microfluidic biochip may be include:

Step S41, determine the target duty cycle after the heating device is started based on the temperature of the microfluidic biochip; and

Step S42: Control the operation of the heating device according to the target duty cycle.

It will be appreciated that the target duty cycle of the heating device can be expressed in percentage. For example, when the target duty cycle of the heating device is 40%, it means that within the preset time period, the heating device starts heating for 40% of the time and stops heating for 60% of the time.

When the temperature of the microfluidic biochip is low, the control method of the present invention also selects different target duty cycles of the heating device according to the temperature of the microfluidic biochip. On the one hand, when the temperature of the microfluidic biochip is too low, When the temperature of the microfluidic biochip is slightly lower, a larger target duty cycle can be used to increase the heating speed, thereby shortening the time consumed by the entire detection and improving the detection efficiency. On the other hand, when the temperature of the microfluidic biochip is slightly lower, it can be used The smaller target duty cycle prevents the temperature of the microfluidic biochip from excessively increasing due to excessive heating speed, thereby avoiding large fluctuations in the temperature of the microfluidic biochip and increasing the temperature of the microfluidic biochip. Control stability.

Specifically, a correspondence table between the temperature of the microfluidic biochip and the duty cycle of the heating device can be pre-stored in the microfluidic detection system, and it only needs to be matched to the corresponding target duty cycle based on the obtained temperature value. Can.

In some embodiments, when the temperature of the microfluidic biochip is less than the first preset temperature value, the target duty cycle of the heating device is the first preset duty cycle; when the temperature of the microfluidic biochip is greater than or equal to the th When a preset temperature value is less than or equal to the second preset temperature value, the target duty cycle of the heating device is the second preset duty cycle; when the temperature of the microfluidic biochip is greater than the second preset temperature value, the heating device The target duty cycle of the device is the third preset duty cycle. Wherein, the first preset temperature value is less than the second preset temperature value, the second preset temperature value is less than the minimum endpoint value of the first preset temperature range, the first preset duty cycle, the second preset duty cycle, The third preset duty cycle decreases sequentially.

That is to say, the lower the temperature of the microfluidic biochip, the greater the target duty cycle of the heating device; the higher the temperature of the microfluidic biochip, the smaller the target duty cycle of the heating device.

Specifically, FIG. 4 is a schematic diagram of a control method of a microfluidic detection system according to yet another embodiment of the present invention. Sexual flow chart. In some embodiments, the control method of the present invention includes:

Step S11, obtain the temperature of the microfluidic biochip;

Step S411, determine whether the temperature of the microfluidic biochip is less than the first preset temperature value; if so, go to step S421; if not, go to step S412;

Step S421, control the operation of the heating device according to the first preset duty cycle;

Step S412, determine whether the temperature of the microfluidic biochip is lower than the second preset temperature value; if yes, go to step S422; if not, go to step S423;

Step S422, control the operation of the heating device according to the second preset duty cycle;

Step S423: Control the operation of the heating device according to the third preset duty cycle.

In some embodiments, after heating the microfluidic biochip, the control method of the present invention further includes:

Continue to obtain the temperature of the microfluidic biochip;

Determine whether the obtained temperature of the microfluidic biochip is greater than the maximum endpoint value of the second preset temperature range; if so, stop the heating device; if not, continue to heat the microfluidic biochip;

Obtain the temperature of the microfluidic biochip again;

Determine whether the temperature of the microfluidic biochip obtained again is less than the minimum endpoint value of the second preset temperature range; if so, restart the heating device to reheat the microfluidic biochip; if not, keep the heating device stop state.

That is to say, after heating the microfluidic biochip, it is necessary to continue to detect the temperature of the microfluidic biochip to prevent the temperature of the microfluidic biochip from rising excessively.

Further, the step of continuing to heat the microfluidic biochip may include:

The operation of the heating device is controlled according to the fourth preset duty cycle.

Further, the step of restarting the heating device to reheat the microfluidic biochip may include:

Start the heating device, and control the operation of the heating device according to the fourth preset duty cycle.

Wherein, the fourth preset duty cycle is less than or equal to the third preset duty cycle.

That is to say, after heating the microfluidic biochip, the temperature of the microfluidic biochip is close to the required First preset temperature range. If you need to continue heating the microfluidic biochip, you need to use a smaller third preset duty cycle or a fourth preset duty cycle smaller than the third preset duty cycle to control the operation of the heating device to avoid heating. The faster speed causes the temperature of the microfluidic biochip to increase uncontrollably and excessively, thus further preventing undesired fluctuations in the temperature of the microfluidic biochip.

Similarly, when heating of the microfluidic biochip is stopped, the temperature of the microfluidic biochip is already at or closer to the required first preset temperature range. If the temperature of the microfluidic biochip is slightly lowered and the microfluidic biochip needs to be heated again, it is also necessary to use a smaller third preset duty cycle or a fourth preset duty cycle smaller than the third preset duty cycle. The air ratio controls the operation of the heating device to prevent the temperature of the microfluidic biochip from uncontrollably rising too much due to a fast heating speed, thereby further avoiding undesired fluctuations in the temperature of the microfluidic biochip.

Specifically, Figure 5 is a schematic flow chart of a control method of a microfluidic detection system according to yet another embodiment of the present invention. Referring to Figure 5, the control method of the present invention includes:

Step S11, obtain the temperature of the microfluidic biochip;

Step S51, continue to obtain the temperature of the microfluidic biochip;

Step S52, determine whether the obtained temperature of the microfluidic biochip is greater than the maximum endpoint value of the second preset temperature range; if so, go to step S54; if not, go to step S53;

Step S53, control the operation of the heating device according to the fourth preset duty cycle, and return to step S51;

Step S54, stop the heating device;

Step S55, obtain the temperature of the microfluidic biochip again;

Step S56, determine whether the obtained temperature of the microfluidic biochip is less than the minimum endpoint value of the second preset temperature range; if so, go to step S57; if not, return to step S55;

Step S57: Start the heating device, control the operation of the heating device according to the fourth preset duty cycle, and return to step S51.

In some embodiments, the maximum endpoint value of the first preset temperature range is the sum of the preset temperature threshold and the first temperature difference value; the minimum endpoint value of the first preset temperature range is the sum of the preset temperature threshold and the first temperature difference value. difference; the maximum endpoint value of the second preset temperature range is the sum of the preset temperature threshold and the second temperature difference value; the minimum endpoint value of the second preset temperature range is the difference between the preset temperature threshold and the second temperature difference value. Wherein, the first temperature difference value is greater than the second temperature difference value. That is to say, the first preset temperature range has a larger range. This is because when the microfluidic biochip is not heated at the beginning, the microfluidic biochip does not have any heat source and its temperature is stable. After heating the microfluidic biochip, the heating device will generate a certain amount of heat. Even if the heating device stops, a certain amount of residual heat will be transferred to the microfluidic biochip. Therefore, the second preset temperature range prompts the heating device to stop. The maximum endpoint value is slightly smaller than the maximum endpoint value of the first preset temperature range. In this way, after the heating device is stopped, the microfluidic biochip can be prevented from absorbing the waste heat of the heating device and causing the temperature to rise excessively.

It can be understood that the above-mentioned preset temperature threshold can be the optimal reaction temperature of the detection reagent used in the microfluidic detection system. When the detection reagents used are different, the values of the above-mentioned preset temperature thresholds are also different.

In some embodiments, before acquiring the first indication signal or the second indication signal, the control method of the present invention further includes:

Receive a chip installation signal indicating that the microfluidic biochip is installed.

That is to say, the temperature of the microfluidic biochip should be monitored only after the microfluidic biochip is installed to avoid incomplete or completely inadequate installation of the microfluidic biochip from affecting the accuracy of temperature acquisition.

It can be understood that the temperature detection of the microfluidic biochip can run through the entire detection process of the microfluidic detection system, that is, from the insertion of the microfluidic biochip to the completion of detection.

The invention also provides a microfluidic detection system for qualitative and/or quantitative detection of preset detection parameters of samples. FIG. 6 is a schematic structural block diagram of a microfluidic detection system according to an embodiment of the present invention, and FIG. 7 is a schematic structural diagram of a microfluidic detection system according to an embodiment of the present invention. Referring to FIGS. 6 and 7 , the microfluidic detection system 10 of the present invention may specifically include a microfluidic biochip 12 , a prompting device 16 and a heating device 17 . The microfluidic biochip 12 is used for sample liquid and detection reagents to react within it. The prompting device 16 is used to send reminder information. The heating device 17 is used to heat the microfluidic biochip 12 .

In particular, the microfluidic detection system 10 also includes a control device 18, and the prompting device 16 and the heating device 17 are both connected to the control device 18. The control device 18 includes a processor 181 and a memory 182, which stores There is a machine executable program 183, and when executed by the processor 181, the machine executable program 183 is used to implement the control method described in any of the above embodiments.

Specifically, the processor 181 may be a central processing unit (CPU for short), or a digital processing unit, or the like. The processor 181 sends and receives data through the communication interface. The memory 182 is used to store programs executed by the processor 181 . Memory 182 is any medium that can be used to carry or store desired program code in the form of instructions or data structures and that can be accessed by a computer, or can be a combination of multiple memories. The above-mentioned machine executable program 183 can be downloaded from a computer-readable storage medium to a corresponding computing/processing device or to a computer or external storage device via a network (such as the Internet, a local area network, a wide area network and/or a wireless network).

Furthermore, the microfluidic detection system 10 also includes a temperature sensor 19 , which is disposed at the microfluidic biochip 12 and used to obtain the temperature of the microfluidic biochip 12 .

Specifically, the heating device 17 and the temperature sensor 19 are both arranged outside the microfluidic biochip 12 and fixed on the mounting mechanism of the microfluidic biochip 12 .

In some embodiments, the microfluidic detection system 10 may also optionally include a buffer storage tank 11 for storing detection buffer, a sample stage 13 for placing the sample cup 2, and a buffer storage tank for driving the buffer storage tank. The buffer in 11 flows to the buffer driving device 14 of the sample cup, the sample liquid driving device 15 for driving the sample liquid into the microfluidic biochip 12, and so on. Among them, the sample cup 2 is used for users to place samples, receive buffer driven by the buffer driving device, etc.

Taking the microfluidic detection system 10 with the above structure as an example, after the user puts the sample into the sample cup 2, the buffer driving device 14 drives the buffer in the buffer storage tank 11 to flow to the sample cup 2. After the sample and the buffer are mixed, A sample liquid is formed, and the sample liquid driving device 15 drives the sample liquid into the microfluidic biochip 12 and then completes reactions, detection, etc. in the microfluidic biochip 12 .

The present invention also provides a refrigerator. Figure 8 is a schematic structural diagram of the refrigerator according to an embodiment of the present invention. The refrigerator 1 of the present invention includes a box body 20 and a door body 30 . The box 20 defines a storage compartment for storing items. The door 30 is connected to the box 20 to open and/or close the storage compartment. In particular, the refrigerator 1 also includes the microfluidic detection system 10 described in any of the above embodiments, and the microfluidic detection system 10 is provided on the box 20 or the door 30 .

Further, the microfluidic detection system 10 can be electrically connected to the electrical control device of the refrigerator 1 to provide power for the microfluidic detection system 1 through the electrical control device and/or allow communication between the electrical control device and the microfluidic detection system 1 Transmission signal.

Preferably, the microfluidic detection system 10 is preferably arranged on the door body 30, which is not only more convenient to operate, but also Moreover, the original storage space in the box 20 will not be occupied, and the storage capacity of the refrigerator 1 itself will not be affected.

The refrigerator 1 of the present invention is integrated with a microfluidic detection system 10, which facilitates the popularization of food detection into ordinary family life, and facilitates the use of the environment in the refrigerator 1 to cool down the microfluidic biochip 12, thereby improving user convenience.

It should be noted that in the description of the present invention, unless otherwise clearly stated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection or a detachable connection. Connection, or integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, an indirect connection through an intermediate medium, or an internal connection between two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. Furthermore, the terms “first”, “second” and “third” are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

By now, those skilled in the art will appreciate that, although a number of exemplary embodiments of the present invention have been shown and described in detail herein, the disclosed embodiments may still be practiced in accordance with the present invention without departing from the spirit and scope of the present invention. The content directly identifies or leads to many other variations or modifications consistent with the principles of the invention. Accordingly, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.

Claims

A control method for a microfluidic detection system, which is used to conduct qualitative and/or quantitative detection of preset detection parameters of a sample, and the microfluidic detection system includes a sample liquid supply and a A microfluidic biochip in which detection reagents react, and the control method includes:

When a first indication signal is obtained, a reminder message indicating that the temperature of the microfluidic biochip is too high is sent, and the first indication signal is used to indicate that the temperature of the microfluidic biochip is too high; and

When a second indication signal is obtained, the microfluidic biochip is heated, and the second indication signal is used to indicate that the temperature of the microfluidic biochip is too low.
The control method according to claim 1, wherein the first indication signal and the second indication signal are generated in the following manner:

Obtain the temperature of the microfluidic biochip;

The first indication signal is generated when the temperature of the microfluidic biochip is higher than the maximum endpoint value of the first preset temperature range;

The second indication signal is generated when the temperature of the microfluidic biochip is lower than the minimum endpoint value of the first preset temperature range.
The control method according to claim 2, wherein the microfluidic detection system further includes a heating device for heating the microfluidic biochip; and

The step of heating the microfluidic biochip includes:

Determine the target duty cycle after the heating device is activated according to the temperature of the microfluidic biochip; and

The operation of the heating device is controlled according to the target duty cycle.
The control method according to claim 3, wherein,

When the temperature of the microfluidic biochip is less than the first preset temperature value, the target duty cycle of the heating device is the first preset duty cycle;

When the temperature of the microfluidic biochip is greater than or equal to the first preset temperature value and less than or equal to the second preset temperature value, the target duty cycle of the heating device is the second preset duty cycle;

When the temperature of the microfluidic biochip is greater than the second preset temperature value, the purpose of the heating device is The standard duty cycle is the third preset duty cycle; where

The first preset temperature value is less than the second preset temperature value, the second preset temperature value is less than the minimum endpoint value of the first preset temperature range, the first preset duty cycle, The second preset duty cycle and the third preset duty cycle decrease in sequence.
The control method according to claim 4, wherein after heating the microfluidic biochip, the control method further includes:

Continue to obtain the temperature of the microfluidic biochip;

Determine whether the obtained temperature of the microfluidic biochip is greater than the maximum endpoint value of the second preset temperature range; if so, stop the heating device; if not, continue to heat the microfluidic biochip ;

Obtain the temperature of the microfluidic biochip again;

Determine whether the temperature of the microfluidic biochip obtained again is less than the minimum endpoint value of the second preset temperature range; if so, restart the heating device to reheat the microfluidic biochip. ; If not, maintain the stopped state of the heating device.
The control method according to claim 5, wherein,

The step of continuing to heat the microfluidic biochip includes:

Control the operation of the heating device according to a fourth preset duty cycle;

The step of restarting the heating device to reheat the microfluidic biochip includes:

Start the heating device and control the operation of the heating device according to the fourth preset duty cycle; wherein

The fourth preset duty cycle is less than or equal to the third preset duty cycle.
The control method according to claim 5, wherein,

The maximum endpoint value of the first preset temperature range is the sum of the preset temperature threshold and the first temperature difference value;

The minimum endpoint value of the first preset temperature range is the difference between the preset temperature threshold and the first temperature difference value;

The maximum endpoint value of the second preset temperature range is the sum of the preset temperature threshold and the second temperature difference value;

The minimum endpoint value of the second preset temperature range is the difference between the preset temperature threshold and the second temperature difference value; and

The first temperature difference value is greater than the second temperature difference value.
The control method according to claim 1, wherein before acquiring the first indication signal or the second indication signal, the control method further includes:

Receive a chip installation signal indicating that the microfluidic biochip has been installed.
A microfluidic detection system for qualitative and/or quantitative detection of preset detection parameters of samples, and the microfluidic detection system includes:

Microfluidic biochips are used for sample fluids and detection reagents to react within them;

Prompt device for issuing reminder messages;

A heating device for heating the microfluidic biochip; and

A control device, including a processor and a memory, where a machine executable program is stored in the memory, and when the machine executable program is executed by the processor, it is used to implement the control according to any one of claims 1-8 method.
A refrigerator including:

A box defining a storage compartment for storing items;

A door body connected to the box body to open and/or close the storage compartment; and

The microfluidic detection system according to claim 9, is arranged on the box or the door.