CN116953265A - Pesticide residue detection system, control method thereof and refrigerator - Google Patents
Pesticide residue detection system, control method thereof and refrigerator Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 216
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- 239000007788 liquid Substances 0.000 claims description 34
- 238000002835 absorbance Methods 0.000 claims description 24
- 230000008569 process Effects 0.000 claims description 24
- 238000003780 insertion Methods 0.000 claims description 22
- 230000037431 insertion Effects 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 18
- 108090000790 Enzymes Proteins 0.000 claims description 18
- 239000007853 buffer solution Substances 0.000 claims description 18
- 230000005764 inhibitory process Effects 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 238000009434 installation Methods 0.000 claims description 9
- 230000015654 memory Effects 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 9
- 239000012916 chromogenic reagent Substances 0.000 claims description 4
- 230000001960 triggered effect Effects 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 230000002411 adverse Effects 0.000 abstract description 3
- 239000000872 buffer Substances 0.000 description 16
- 238000005259 measurement Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 235000012055 fruits and vegetables Nutrition 0.000 description 5
- 230000001976 improved effect Effects 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
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- 239000012530 fluid Substances 0.000 description 1
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- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D23/00—General constructional features
- F25D23/12—Arrangements of compartments additional to cooling compartments; Combinations of refrigerators with other equipment, e.g. stove
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/025—Fruits or vegetables
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Abstract
The invention relates to an agricultural residue detection system, a control method thereof and a refrigerator. The control method of the invention comprises the following steps: after receiving the pesticide residue detection starting instruction, starting a wavelength initialization flow of the light detection unit; the wavelength initialization flow includes: starting a light source of the light detection unit; detecting a parameter value of a preset parameter related to the wavelength of light emitted by the light source, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting the duty ratio of the PWM control signal of the light source, wherein the target parameter value is set to be a preset wavelength value when the preset parameter of the light source is the target parameter value; and recording the duty ratio of the PWM control signal as a target duty ratio when the preset parameter of the light source is a target parameter value. Therefore, during detection, the duty ratio of the PWM control signal of the light source can be directly set to be the target duty ratio, the wavelength of light emitted by the light source is ensured to be the optimal preset wavelength value, and adverse effects on a detection result due to wavelength fluctuation are avoided.
Description
Technical Field
The invention relates to a refrigeration technology, in particular to an agricultural residue detection system, a control method thereof and a refrigerator.
Background
Along with the gradual improvement of convenience of living and shopping, the ways of purchasing fruits and vegetables by users are more and more abundant, such as off-line supermarkets, marts, on-line beauty balls, jindong, and ali. In the face of rich vegetable purchasing ways, users also gradually pay attention to whether fruits and vegetables contain pesticide residues. In fixed places such as supermarkets, fruits and vegetables have a certain pesticide residue inspection, but the fruits and vegetables are incomplete; and some purchased fruits and vegetables cannot guarantee reliable pesticide residue detection at all. Pesticide residue detection is taken as a relatively professional detection means, and cannot be finished at home of common users at present. The applicant has previously proposed a refrigerator integrated with an agricultural residue detection system, which is capable of performing agricultural residue detection on food materials by using an enzyme inhibition method on the refrigerator.
However, when the enzyme inhibition method is used for pesticide residue detection, the wavelength of light emitted by the light source is required to be maintained at a certain wavelength value or a certain wavelength range, but at present, the light source is controlled only by using simple switch control, and when the current or voltage of the light source fluctuates, the wavelength drift is easy to occur, so that the wavelength in the detection process is not in the required range, and the detection result is inaccurate.
Disclosure of Invention
An object of the first aspect of the present invention is to overcome at least one of the drawbacks of the prior art, and to provide a control method of an agricultural residue detection system capable of ensuring that the wavelength of light emitted from a light source is stabilized within a predetermined range during detection.
It is a further object of the first aspect of the invention to reduce the light intensity fluctuations and further improve the accuracy of the detection results.
It is a further object of the first aspect of the invention to reduce the requirements for the uniformity of microfluidic chips.
An object of the second aspect of the present invention is to provide an agricultural residue detection system capable of ensuring that the wavelength of light emitted from a light source is stabilized within a predetermined range during detection.
An object of a third aspect of the present invention is to provide a refrigerator having the above pesticide residue detection system.
According to a first aspect of the present invention, there is provided a control method of an agricultural residue detection system for detecting agricultural residues on a sample by an enzyme inhibition method, the agricultural residue detection system including a light detection unit for detecting absorbance, the control method comprising:
after receiving an pesticide residue detection starting instruction, starting a wavelength initialization flow of the light detection unit; the wavelength initialization process includes:
activating a light source of the light detection unit;
detecting a parameter value of a preset parameter related to the wavelength of light emitted by the light source, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting the duty ratio of a PWM control signal of the light source, wherein the target parameter value is set to be a preset wavelength value when the preset parameter of the light source is the target parameter value; and
and recording the duty ratio of the PWM control signal as a target duty ratio when the preset parameter is the target parameter value.
Optionally, the pesticide residue detection system further comprises a microfluidic chip with a detection pool, the light detection unit further comprises a light intensity sensor, and the light source and the light intensity sensor are respectively arranged at two sides of the microfluidic chip; and is also provided with
The preset parameter is the light intensity detected by the light intensity sensor, and the target parameter value is a target light intensity value.
Optionally, after receiving the pesticide residue detection start instruction and before starting the wavelength initialization procedure, the control method further includes:
detecting whether the microfluidic chip is inserted in place; and
if yes, executing the wavelength initialization flow; if not, sending out chip insertion prompt information, and returning to continuously detect whether the micro-fluidic chip is inserted in place, wherein the chip insertion prompt information is used for reminding a user to insert the micro-fluidic chip into a chip installation position of the pesticide residue detection system.
Optionally, after receiving the pesticide residue detection start instruction and before starting the wavelength initialization procedure, the control method further includes:
detecting whether the microfluidic chip is inserted in place; and
if yes, sending out chip removal prompt information, and returning to continuously detect whether the microfluidic chip is inserted in place, wherein the chip removal prompt information is used for reminding a user to remove the microfluidic chip from the chip installation position of the pesticide residue detection system; and if not, executing the wavelength initialization flow.
Optionally, after the wavelength initialization procedure, the control method further includes:
and sending out chip insertion prompt information, wherein the chip insertion prompt information is used for reminding a user to insert the microfluidic chip into the chip installation position of the pesticide residue detection system.
Optionally, the preset parameter is a driving current of the light source, and the target parameter value is a target current value.
Optionally, after the duty cycle of the PWM control signal is recorded as the target duty cycle when the preset parameter of the light source is the target parameter value, the wavelength initialization procedure further includes:
and turning off the light source of the light detection unit.
Optionally, the pesticide residue detection system further comprises a detection tank containing a chromogenic reagent;
after the wavelength initialization procedure, the control method further includes a detection procedure, where the detection procedure includes:
and after the sample liquid enters the detection cell, starting a light source of the light detection unit, and setting the duty ratio of a PWM control signal of the light source to be the target duty ratio.
Optionally, the pesticide residue detection system further comprises a sample cup for containing a sample liquid and a reaction tank containing an enzyme reagent; and is also provided with
Before the sample liquid enters the detection cell, the detection flow further comprises:
acquiring the weight of a sample in the sample cup;
inputting a corresponding amount of buffer solution into the sample cup according to the weight of the sample in the sample cup;
suspending the sample cup, and then oscillating the sample cup to obtain a sample liquid;
driving a preset amount of sample liquid in the sample cup to enter the reaction tank, and enabling the sample liquid entering the reaction tank to fully react with an enzyme reagent in the reaction tank;
after the light source is started, the detection process further comprises:
and obtaining an pesticide residue detection result of the sample liquid according to the absorbance of the detection pool.
Optionally, the pesticide residue detection starting instruction is triggered to be generated after a sample is placed in a sample cup of the pesticide residue detection system; or alternatively
And the pesticide residue detection starting instruction is generated after a trigger button of the pesticide residue detection system receives a trigger signal.
According to an object of the second aspect of the present invention, there is also provided an agricultural residue detection system for detecting agricultural residue on a sample by an enzyme inhibition method, comprising:
a light detection unit for performing absorbance detection and having a light source for emitting light; and
the control device comprises a processor and a memory, wherein a machine executable program is stored in the memory, and the machine executable program is used for realizing the control method according to any scheme when being executed by the processor.
According to a third aspect of the present invention, there is also provided a refrigerator including:
a case defining a storage compartment therein for storing articles;
the door body is connected with the box body to open and/or close the storage compartment; and
the pesticide residue detection system according to any one of the above schemes is arranged on the box body or the door body.
The control method of the pesticide residue detection system does not immediately start a specific detection flow after receiving the pesticide residue detection starting instruction, but firstly executes the wavelength initialization flow of the light detection unit. In the wavelength initialization process, the duty ratio of the PWM control signal of the light source is continuously adjusted by continuously detecting the parameter value of the preset parameter related to the wavelength of the light emitted by the light source until the duty ratio of the PWM control signal of the light source is found out when the parameter value of the preset parameter reaches the target parameter value (the wavelength of the light emitted by the corresponding light source is the preset wavelength value), and is recorded as the target duty ratio. That is, the present invention first determines the target duty ratio of the PWM control signal of the light source when the wavelength of the light emitted by the light source is the preset wavelength value through the wavelength initialization procedure, thereby, in the detection procedure, the duty ratio of the PWM control signal of the light source can be directly set to the target duty ratio, ensuring that the wavelength of the light emitted by the light source is the optimal preset wavelength value, avoiding the adverse effect on the detection result caused by the wavelength fluctuation of the light emitted by the light source, and ensuring the accuracy of the detection result.
Further, when the preset parameter is selected as the light intensity detected by the light intensity sensor, the inventor recognizes that since the light source and the light intensity sensor are respectively disposed at both sides of the microfluidic chip, whether the microfluidic chip is inserted or not may have a certain influence on the light intensity detected by the light intensity sensor. Therefore, the wavelength initialization process is carried out after the micro-fluidic chip is inserted, the interference of the micro-fluidic chip, which is an interference factor, on the light intensity is eliminated, the light intensity fluctuation is smaller in the subsequent detection process, and the accuracy of the detection result is further improved.
Further, the inventor further recognizes that when the preset parameter is selected as the light intensity detected by the light intensity sensor, if the wavelength initialization process is performed after the microfluidic chip is inserted, although the interference factor can be reduced and the light intensity fluctuation can be reduced, the manufacturing consistency requirement on the microfluidic chip is higher, that is, the difference between different microfluidic chips needs to be minimum or even zero, and the manufacturing requirement and difficulty of the microfluidic chip are increased. If the consistency of the microfluidic chip is poor, it is also difficult to completely avoid light intensity fluctuations. Therefore, in other embodiments, the wavelength initialization process is performed before the microfluidic chip is inserted, so that errors caused by poor consistency of the microfluidic chip can be eliminated, and the requirement for consistency of the microfluidic chip is reduced.
The above, as well as additional objectives, advantages, and features of the present invention will become apparent to those skilled in the art from the following detailed description of a specific embodiment of the present invention when read in conjunction with the accompanying drawings.
Drawings
Some specific embodiments of the invention will be described in detail hereinafter by way of example and not by way of limitation with reference to the accompanying drawings. The same reference numbers will be used throughout the drawings to refer to the same or like parts or portions. It will be appreciated by those skilled in the art that the drawings are not necessarily drawn to scale. In the accompanying drawings:
FIG. 1 is a schematic block diagram of an pesticide residue detection system according to one embodiment of the present invention;
FIG. 2 is a partially exploded view of an agricultural residue detection system according to an embodiment of the present invention;
FIG. 3 is a schematic flow chart of a wavelength initialization procedure according to one embodiment of the invention;
FIG. 4 is a schematic flow chart of a wavelength initialization procedure according to another embodiment of the invention;
FIG. 5 is a schematic flow chart diagram of a control method of an pesticide residue detection system according to one embodiment of the present invention;
FIG. 6 is a schematic flow chart diagram of a control method of an pesticide residue detection system according to another embodiment of the present invention;
FIG. 7 is a schematic flow chart diagram of a detection flow in accordance with one embodiment of the present invention;
FIG. 8 is a schematic block diagram of an agricultural residue detection system according to an embodiment of the present invention;
fig. 9 is a schematic structural view of a refrigerator according to an embodiment of the present invention.
Detailed Description
The invention firstly provides a control method of an agricultural residue detection system, which is used for detecting agricultural residue on a sample by utilizing an enzyme inhibition method. Fig. 1 is a schematic structural view of an agricultural residue detection system according to an embodiment of the present invention, and fig. 2 is a partially exploded structural view of the agricultural residue detection system according to an embodiment of the present invention. The pesticide residue detection system 10 of the present invention includes a light detection unit for performing absorbance detection, the light detection unit including at least a light source 161.
In particular, the control method of the present invention comprises:
and after receiving the pesticide residue detection starting instruction, starting a wavelength initialization flow of the light detection unit.
Fig. 3 is a schematic flow chart of a wavelength initialization procedure according to one embodiment of the invention. Referring to fig. 3, the wavelength initialization procedure of the present invention includes:
step S31, the light source 161 of the light detection unit is activated;
step S32, detecting a parameter value of a preset parameter related to a wavelength of light emitted from the light source 161, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting a duty ratio of a PWM control signal of the light source 161, wherein the target parameter value is set such that the wavelength of light emitted from the light source 161 is the preset wavelength value when the preset parameter of the light source 161 is the target parameter value; and
step S33, the duty ratio of the PWM control signal is recorded as the target duty ratio when the preset parameter is the target parameter value.
The control method of the pesticide residue detection system 10 of the present invention does not immediately start a specific detection flow after receiving the pesticide residue detection start instruction, but first executes a wavelength initialization flow of the light detection unit. In the wavelength initialization process, the duty ratio of the PWM control signal of the light source 161 is continuously adjusted by continuously detecting the parameter value of the preset parameter related to the wavelength of the light emitted by the light source 161 until the duty ratio of the PWM control signal of the light source 161 is found such that the parameter value of the preset parameter reaches the target parameter value (the wavelength of the light emitted by the corresponding light source 161 is the preset wavelength value), and is recorded as the target duty ratio. That is, the present invention first determines the target duty ratio of the PWM control signal of the light source 161 when the wavelength of the light emitted from the light source 161 is the preset wavelength value through the wavelength initialization procedure, thereby, in the detection procedure, the duty ratio of the PWM control signal of the light source 161 can be directly set to the target duty ratio, ensuring that the wavelength of the light emitted from the light source 161 is the optimal preset wavelength value, avoiding the adverse effect on the detection result caused by the wavelength fluctuation of the light emitted from the light source 161, and ensuring the accuracy of the detection result.
It can be appreciated that the predetermined wavelength value is the wavelength value most favorable for detection. For example, in the case of pesticide residue detection by the enzyme inhibition method, the wavelength of light emitted from the light source 161 is required to be optimal at 412nm, and the preset wavelength value is 412nm. Accordingly, when the light source 161 emits light having a wavelength of 412nm, the parameter value of the predetermined parameter is a target parameter value.
Fig. 4 is a schematic flow chart of a wavelength initialization procedure according to another embodiment of the invention. Referring to fig. 4, in other embodiments, after the duty cycle of the PWM control signal is recorded as the target duty cycle when the preset parameter of the light source 161 is the target parameter value, the wavelength initialization procedure of the present invention further includes:
step S34 turns off the light source 161 of the light detection unit.
That is, when the wavelength initialization process ends, the light source 161 is in an off state. The inventors have recognized that after the end of the wavelength initialization procedure, a series of operations such as weighing, buffer injection, sample liquid suction, etc. are required before absorbance detection, and these operations do not require the participation of the light detection unit, so that the power consumption can be reduced by turning off the light source 161.
In some embodiments, the pesticide residue detection initiation command is triggered to be generated after the sample is placed in the sample cup 2 of the pesticide residue detection system 10 is detected. That is, after the user puts the sample into the sample cup 2, the pesticide residue detection starting instruction can be automatically generated, and the intelligent degree is high.
In other embodiments, the pesticide residue detection initiation instruction is generated after a trigger signal is received by a trigger button of pesticide residue detection system 10. That is, the user generates the pesticide residue detection starting instruction after operating the trigger button in a pressing or touching mode, and the pesticide residue detection starting instruction is more in line with the use intention of the user.
In some embodiments, the pesticide residue detection system further includes a microfluidic chip 12 having a detection cell 121, the light detection unit further includes a light intensity sensor 162 for detecting light intensity, and the light source 161 and the light intensity sensor 162 are disposed at both sides of the microfluidic chip, respectively. It will be appreciated that the light intensity is closely related to the wavelength of the light, and thus, in these embodiments, the predetermined parameter may be the light intensity detected by the light intensity sensor 162, and accordingly, the target parameter value is the target light intensity value.
In a further embodiment, after receiving the pesticide residue detection start command and before starting the wavelength initialization process, the control method of the present invention further includes:
detecting whether a microfluidic chip is inserted in place; and
if yes, starting a wavelength initialization flow; if not, sending out chip insertion prompt information, and returning to continuously detect whether the micro-fluidic chip is inserted in place, wherein the chip insertion prompt information is used for reminding a user to insert the micro-fluidic chip into the chip installation position of the pesticide residue detection system.
Specifically, fig. 5 is a schematic flow chart of a control method of the pesticide residue detection system according to one embodiment of the present invention. Referring to fig. 5, in some embodiments, the control method of the present invention includes:
step S10, an agricultural residue detection starting instruction is received;
step S20, detecting whether the microfluidic chip 12 is inserted in place; if yes, go to step S30, if no, go to step S40;
step S30, starting a wavelength initialization flow;
step S40, sending out chip insertion prompt information, and returning to step S20.
When the preset parameter is selected as the light intensity detected by the light intensity sensor 162, the inventor has recognized that since the light source 161 and the light intensity sensor 162 are respectively disposed at both sides of the microfluidic chip 12, the insertion or non-insertion of the microfluidic chip 12 may have a certain effect on the light intensity detected by the light intensity sensor 162. Therefore, the wavelength initialization process is carried out after the micro-fluidic chip 12 is inserted, the interference of the interference factor of the micro-fluidic chip 12 on the light intensity is eliminated, the light intensity fluctuation is smaller in the subsequent detection process, and the accuracy of the detection result is further improved.
It is understood that the step S30 may specifically include the steps S31 to S33 or the steps S31 to S34.
In another further embodiment, after receiving the pesticide residue detection start instruction and before starting the wavelength initialization process, the control method of the present invention further includes:
detecting whether the microfluidic chip 12 is inserted in place; and
if yes, sending out chip removal prompt information, and returning to continuously detect whether the micro-fluidic chip 12 is inserted in place, wherein the chip removal prompt information is used for reminding a user to remove the micro-fluidic chip 12 from the chip installation position of the pesticide residue detection system; if not, the wavelength initialization flow is started.
Specifically, fig. 6 is a schematic flow chart of a control method of the pesticide residue detection system according to another embodiment of the present invention. Referring to fig. 6, in some embodiments, the control method of the present invention includes:
step S10, an agricultural residue detection starting instruction is received;
step S20, detecting whether the microfluidic chip 12 is inserted in place; if yes, go to step S40, if no, go to step S30;
step S30, executing a wavelength initialization flow;
step S40', send out the chip removes the prompt message, and return to step S20.
The inventor further recognized that when the preset parameter is selected as the light intensity detected by the light intensity sensor 162, if the wavelength initialization process is performed after the microfluidic chip 12 is inserted, the interference factor can be reduced, and the light intensity fluctuation can be reduced, but the manufacturing consistency requirement on the microfluidic chip 12 is higher, that is, the difference between different microfluidic chips 12 needs to be minimum or even zero, and the manufacturing requirement and difficulty of the microfluidic chip 12 are increased. If the uniformity of the microfluidic chip 12 is poor, it is also difficult to completely avoid the fluctuation of the light intensity. For this reason, in other embodiments, the wavelength initialization process is performed before the microfluidic chip 12 is inserted, so that errors caused by poor uniformity of the microfluidic chip 12 can be eliminated, and the requirement for uniformity of the microfluidic chip 12 is reduced.
Further, after the wavelength initialization procedure, the control method of the present invention further includes:
and sending out chip insertion prompt information, wherein the chip insertion prompt information is used for reminding a user to insert the microfluidic chip 12 into the chip installation position of the pesticide residue detection system.
That is, in the embodiment in which the wavelength initialization process is performed before the insertion of the microfluidic chip 12, the user needs to be reminded of the insertion of the microfluidic chip 12 in time after the end of the wavelength initialization process, so as to perform pesticide residue detection.
Specifically, referring to fig. 6, after step S30, the control method of the present invention further includes:
step S50, judging whether the wavelength initialization flow is finished; if yes, go to step S60, if no, return to and continue to judge; and
step S60, sending out chip insertion prompt information.
The inventors have realized that the wavelength of the light emitted by the light source 161 is also related to the driving current of the light source 161. Therefore, in other embodiments, the predetermined parameter may be a driving current of the light source 161, and accordingly, the target parameter value is a target current value.
Since the driving current of the light source 161 has no relation with the insertion or not of the microfluidic chip 12, the insertion state of the microfluidic chip 12 does not affect the wavelength initialization process, the wavelength fluctuation in the subsequent detection process is smaller, and the detection accuracy is higher.
In some embodiments, the pesticide residue detection system 10 further includes a detection cell 121 containing a chromogenic reagent, and the detection cell 121 may be formed in the microfluidic chip 12. In these embodiments, after the end of the wavelength initialization procedure, the control method of the present invention further includes: and executing a detection flow.
Specifically, in the embodiment shown in fig. 5, after step S30, the control method of the present invention further includes:
step S50, judging whether the wavelength initialization flow is finished; if yes, go to step S80, if not, return to and continue to judge; and
step S80, a detection procedure is performed.
In the embodiment shown in fig. 6, after step S60, the control method of the present invention further includes:
step S70, judging whether the microfluidic chip is installed in place; if yes, go to step S80, if not, return to and continue to judge; and
step S80, a detection procedure is performed.
Further, the detection process of the present invention specifically may include:
after the sample liquid enters the detection cell 121, the light source 161 of the light detection unit is activated, and the duty ratio of the PWM control signal of the light source 161 is set to the target duty ratio.
That is, in detecting absorbance of the detection cell 121 with the light detection unit, the duty ratio of the PWM control signal of the light source 161 may be directly set to the target duty ratio recorded in the wavelength initialization flow. In this way, the parameter value of the preset parameter related to the wavelength of the light emitted by the light source 161 can be directly adjusted to the preset parameter value, so that the wavelength of the light emitted by the light source 161 is ensured to be the preset wavelength value, and the detection effect is optimal.
In some embodiments, the pesticide residue detection system 10 further includes a sample cup 2 for holding a sample liquid, and a reaction cell 122 holding an enzyme reagent. In these embodiments, the detection procedure of the present invention further comprises, prior to the sample fluid entering the detection cell 121:
acquiring the weight of the sample in the sample cup 2;
inputting a corresponding amount of buffer solution into the sample cup 2 according to the weight of the sample in the sample cup 2;
suspending the sample cup 2, and then oscillating the sample cup 2 to obtain a sample liquid;
driving a preset amount of sample liquid in the sample cup 2 into the reaction tank 122, and enabling the sample liquid entering the reaction tank 122 to fully react with enzyme reagents in the reaction tank 122;
after the light source 161 is activated, the detection process further includes:
and obtaining the pesticide residue detection result of the sample liquid according to the absorbance of the detection cell 121.
Specifically, fig. 7 is a schematic flow chart of a detection flow according to one embodiment of the present invention. Referring to fig. 7, the detection procedure step S80 of the present invention may specifically include:
step S81, obtaining the weight of the sample in the sample cup 2;
step S82, inputting a corresponding amount of buffer solution into the sample cup 2 according to the weight of the sample in the sample cup 2;
step S83, oscillating the sample cup 2 after suspending the sample cup 2 to obtain a sample liquid;
step S84, driving a preset amount of sample liquid in the sample cup 2 into the reaction tank 122, and enabling the sample liquid entering the reaction tank 122 to fully react with the enzyme reagent in the reaction tank 122;
step S85, driving the sample liquid fully reacted with the enzyme reagent in the reaction tank 122 to enter the detection tank 121;
step S86 of starting the light source 161 of the light detection unit and setting the duty ratio of the PWM control signal of the light source 161 to a target duty ratio;
step S87, the pesticide residue detection result of the sample liquid is obtained according to the absorbance of the detection pool 121.
According to the invention, after a corresponding amount of buffer solution is input into the sample cup 2, the sample liquid is suspended so as to separate from the weighing module 13 for acquiring weight, and then the sample cup 2 is oscillated so as to enable pesticide residues on a sample to be fully dissolved into the buffer solution, so that vibration of the sample cup 2 is not transmitted to the weighing module 13, the weighing precision of the weighing module 13 is not affected, high-precision measurement of the sample weight is ensured, and the accuracy of pesticide residue detection results is further improved.
Specifically, in step S84, the sample liquid may be repeatedly pumped to cause the sample liquid to sufficiently react with the enzyme reagent in the reaction cell 122 in such a manner that the sample liquid is made to creep back and forth in the reaction cell 122 a plurality of times.
In some embodiments, the step S87 of obtaining the pesticide residue detection result of the sample liquid according to the absorbance of the detection cell 121 may specifically include:
acquiring light intensity signals for representing the intensity of light transmitted through the detection cell 121 at the start of the color reaction in the detection cell 121 and after the completion of the color reaction, respectively;
calculating the light intensity change, wherein the light intensity change is the difference between a light intensity signal obtained after the completion of the color development reaction and a light intensity signal obtained at the beginning of the color development reaction;
determining the absorbance of the detection cell 121 according to the light intensity variation;
calculating to obtain the pesticide residue inhibition rate of the sample liquid according to the determined absorbance of the detection pool 121;
outputting pesticide residue detection results according to the calculated pesticide residue inhibition rate; wherein the method comprises the steps of
The step of determining the absorbance of the detection cell 121 based on the change in light intensity includes:
if the light intensity variation is smaller than the preset minimum light intensity variation, making the absorbance of the detection cell 121 be the preset minimum light intensity variation;
if the light intensity variation is greater than the preset maximum light intensity variation, making the absorbance of the detection cell 121 be the preset maximum light intensity variation;
if the light intensity variation is between the preset minimum light intensity variation and the preset maximum light intensity variation, the absorbance of the detection cell 121 is made equal to the light intensity variation.
The pesticide residue detecting system 10 of the present invention directly sets the absorbance of the detection cell 121 to the preset maximum light intensity variation when the obtained light intensity variation is excessively large (e.g., greater than the preset maximum light intensity variation), directly sets the absorbance of the detection cell 121 to the preset minimum light intensity variation when the obtained light intensity variation is excessively small (e.g., less than the preset minimum light intensity variation), and sets the absorbance of the detection cell 121 to the obtained light intensity variation when the obtained light intensity variation is appropriate. On the one hand, the problem that the calculated pesticide residue inhibition rate deviates from the theoretical range and causes using trouble to a user due to overlarge or overlarge absorbance of the detection pool 121 caused by the reasons that bubbles are generated in the detection pool 121, the sample liquid does not enter the detection pool 121, the chromogenic reagent is invalid and the like can be avoided; on the other hand, the accuracy of the calculated pesticide residue inhibition rate can be ensured when no fault occurs.
Further, if the light intensity variation is smaller than the preset minimum light intensity variation or the light intensity variation is larger than the preset maximum light intensity variation, the control method of the present invention further includes:
and sending out first fault prompt information which is used for prompting a user to detect possible faults and to test again.
Further, when the obtained light intensity variation is smaller than the preset minimum light intensity variation or larger than the preset maximum light intensity variation, there is a possibility that there are faults such as bubbles generated in the detection cell 121, the sample liquid not entering the detection cell 121, the failure of the color-developing reagent, and the like. Although the absorbance of the detection cell 121 is directly assigned as the preset minimum light intensity variation or the preset maximum light intensity variation at this time so that the calculated pesticide residue inhibition ratio is within the theoretical range, the calculated pesticide residue inhibition ratio is not accurate. Therefore, the invention also sends out the first fault prompt information at the moment to prompt the user that the fault possibly occurs in the detection and retests are needed, and the accuracy of the detection result is improved on the premise of ensuring that the use trouble is not brought to the user.
In some embodiments, the pesticide residue detection system 10 further includes a buffer reservoir 11 for storing the detection buffer, and a buffer drive device 14 for driving the buffer in the buffer reservoir 11 to flow into the sample cup 2. In these embodiments, the step S82 of inputting a corresponding amount of buffer into the sample cup 2 according to the weight of the sample in the sample cup 2 may specifically include:
determining a target weight of the buffer solution required according to the weight of the sample in the sample cup 2;
determining a target working time length of the buffer driving device 14 according to the target weight of the required buffer; starting a buffer driving device; and
and controlling the starting and stopping of the buffer driving device according to the actual measurement working time after the buffer driving device is started and the actual measurement weight of the buffer in the sample cup 2.
According to the control method, the target working time length of the buffer solution driving device 14 is determined according to the target weight of the buffer solution required, and then the starting and stopping of the buffer solution driving device are comprehensively controlled according to the actually measured working time length after the buffer solution driving device 14 is started and the actually measured weight of the buffer solution in the sample cup 2, so that the buffer solution amount injected into the sample cup 2 can be controlled more accurately.
Further, the step of controlling the start and stop of the buffer solution driving device 14 according to the actual measured working time after the start of the buffer solution driving device 14 and the actual measured weight of the buffer solution in the sample cup 2 includes:
acquiring the actual measurement working time after the buffer solution driving device 14 is started and the actual measurement weight of the buffer solution in the sample cup 2; and
stopping the buffer driving device 14 when the actual measurement working time of the buffer driving device 14 reaches the target working time or the actual measurement weight of the buffer in the sample cup 2 reaches the preset weight; wherein the method comprises the steps of
The preset weight is equal to or slightly greater than the target weight.
In some embodiments, the step S20 of detecting whether the microfluidic chip 12 is inserted in place may specifically include:
whether the microfluidic chip 12 is mounted in place is determined based on the light intensity signal obtained by the light intensity sensor 162.
Specifically, if the light intensity signal obtained by the light intensity sensor 162 is greater than the third preset light intensity value and less than the second preset light intensity value, it is determined that the microfluidic chip 12 is mounted in place; otherwise, it is determined that the microfluidic chip 12 is not mounted in place.
When the microfluidic chip 12 is inserted, the light intensity sensor 162 is blocked from sensing light, and the light intensity data acquired by the light intensity sensor 162 is correspondingly changed, so that the insertion condition of the microfluidic chip 12 can be judged accordingly. The invention judges the insertion condition of the micro-fluidic chip 12 by utilizing the conventional structure (light detection unit) which is necessary for the pesticide residue detection system 10, the judgment result is more accurate, and the problems of difficult assembly upgrade and higher cost caused by installing additional structural members such as a switch or a switch-like sensor are also avoided.
The invention also provides a pesticide residue detection system 10 for detecting pesticide residues on a sample by using an enzyme inhibition method. FIG. 8 is a schematic block diagram of an agricultural residue detection system according to an embodiment of the present invention. Referring to fig. 8, the pesticide residue detection system 10 of the present invention includes a light detection unit 16 and a control device 18.
The light detection unit 16 is for absorbance detection, and has a light source 161 for emitting light. The control device 18 includes a processor 181 and a memory 182, the memory 182 stores a machine executable program 183, and the machine executable program 183 is used to implement the control method described in any of the above embodiments when executed by the processor 181.
In particular, the processor 181 may be a central processing unit (central processing unit, CPU for short), or a digital processing unit or the like. The processor 181 transmits and receives data through a communication interface. The memory 182 is used for storing programs executed by the processor 181. Memory 182 is any medium that can be used to carry or store desired program code in the form of instructions or data structures and that can be accessed by a computer, and may be a combination of memories. The machine-executable program 183 described above may be downloaded from a computer-readable storage medium to a corresponding computing/processing device or downloaded to a computer or external memory device via a network (e.g., the internet, a local area network, a wide area network, and/or a wireless network).
The present invention also provides a refrigerator, and fig. 9 is a schematic structural view of a refrigerator according to an embodiment of the present invention. The refrigerator 1 of the present invention includes a cabinet 20 and a door 30. The case 20 defines a storage compartment therein for storing articles. The door 30 is connected to the case 20 to open and/or close the storage compartment. In particular, the refrigerator 1 further includes the pesticide residue detection system 10 described in any of the above embodiments, and the pesticide residue detection system 10 is disposed on the refrigerator body 20 or the door body 30.
The refrigerator 1 is integrated with the pesticide residue detection system 10, so that food materials can be conveniently detected and popularized to ordinary family life, and the convenience of use of users is improved.
Further, the pesticide residue detection system 10 may be electrically connected to an electronic control device of the refrigerator 1 to provide power to the pesticide residue detection system 1 through the electronic control device and/or to allow transmission of signals between the electronic control device and the pesticide residue detection system 1.
Preferably, the pesticide residue detection system 10 is preferably arranged on the door body 30, so that the operation is convenient, the original storage space in the box body 20 is not occupied, and the storage capacity of the refrigerator 1 is not affected.
It should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected, can be indirectly connected through an intermediate medium, and can also be communicated with the inside of two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
By now it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been shown and described herein in detail, many other variations or modifications of the invention consistent with the principles of the invention may be directly ascertained or inferred from the present disclosure without departing from the spirit and scope of the invention. Accordingly, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.
Claims (12)
1. A control method of an agricultural residue detection system for detecting agricultural residues on a sample using an enzyme inhibition method, and the agricultural residue detection system includes a light detection unit for detecting absorbance, the control method comprising:
after receiving an pesticide residue detection starting instruction, starting a wavelength initialization flow of the light detection unit; the wavelength initialization process includes:
activating a light source of the light detection unit;
detecting a parameter value of a preset parameter related to the wavelength of light emitted by the light source, and adjusting the parameter value of the preset parameter to a target parameter value by adjusting the duty ratio of a PWM control signal of the light source, wherein the target parameter value is set to be a preset wavelength value when the preset parameter of the light source is the target parameter value; and
and recording the duty ratio of the PWM control signal as a target duty ratio when the preset parameter is the target parameter value.
2. The control method according to claim 1, wherein the pesticide residue detection system further comprises a microfluidic chip with a detection cell, the light detection unit further comprises a light intensity sensor, and the light source and the light intensity sensor are respectively arranged at two sides of the microfluidic chip; and is also provided with
The preset parameter is the light intensity detected by the light intensity sensor, and the target parameter value is a target light intensity value.
3. The control method according to claim 2, wherein after receiving the pesticide residue detection start-up instruction and before starting the wavelength initialization procedure, the control method further comprises:
detecting whether the microfluidic chip is inserted in place; and
if yes, executing the wavelength initialization flow; if not, sending out chip insertion prompt information, and returning to continuously detect whether the micro-fluidic chip is inserted in place, wherein the chip insertion prompt information is used for reminding a user to insert the micro-fluidic chip into a chip installation position of the pesticide residue detection system.
4. The control method according to claim 2, wherein after receiving the pesticide residue detection start-up instruction and before starting the wavelength initialization procedure, the control method further comprises:
detecting whether the microfluidic chip is inserted in place; and
if yes, sending out chip removal prompt information, and returning to continuously detect whether the microfluidic chip is inserted in place, wherein the chip removal prompt information is used for reminding a user to remove the microfluidic chip from the chip installation position of the pesticide residue detection system; and if not, executing the wavelength initialization flow.
5. The control method of claim 4, wherein after the wavelength initialization procedure, the control method further comprises:
and sending out chip insertion prompt information, wherein the chip insertion prompt information is used for reminding a user to insert the microfluidic chip into the chip installation position of the pesticide residue detection system.
6. The control method according to claim 1, wherein,
the preset parameter is the driving current of the light source, and the target parameter value is a target current value.
7. The control method according to claim 1, wherein,
after the duty ratio of the PWM control signal is recorded as the target duty ratio when the preset parameter of the light source is the target parameter value, the wavelength initialization procedure further includes:
and turning off the light source of the light detection unit.
8. The control method according to claim 1, wherein the pesticide residue detection system further comprises a detection cell containing a chromogenic reagent;
after the wavelength initialization procedure, the control method further includes a detection procedure, where the detection procedure includes:
and after the sample liquid enters the detection cell, starting a light source of the light detection unit, and setting the duty ratio of a PWM control signal of the light source to be the target duty ratio.
9. The control method according to claim 8, wherein the pesticide residue detection system further comprises a sample cup for containing a sample liquid, and a reaction cell containing an enzyme reagent; and is also provided with
Before the sample liquid enters the detection cell, the detection flow further comprises:
acquiring the weight of a sample in the sample cup;
inputting a corresponding amount of buffer solution into the sample cup according to the weight of the sample in the sample cup;
suspending the sample cup, and then oscillating the sample cup to obtain a sample liquid;
driving a preset amount of sample liquid in the sample cup to enter the reaction tank, and enabling the sample liquid entering the reaction tank to fully react with an enzyme reagent in the reaction tank;
after the light source is started, the detection process further comprises:
and obtaining an pesticide residue detection result of the sample liquid according to the absorbance of the detection pool.
10. The control method according to claim 1, wherein,
the pesticide residue detection starting instruction is triggered to be generated after a sample is placed in a sample cup of the pesticide residue detection system; or alternatively
And the pesticide residue detection starting instruction is generated after a trigger button of the pesticide residue detection system receives a trigger signal.
11. An agricultural residue detection system for detecting agricultural residues on a sample by using an enzyme inhibition method, and comprising:
a light detection unit for performing absorbance detection and having a light source for emitting light; and
control device comprising a processor and a memory, said memory having stored therein a machine executable program, and said machine executable program when executed by said processor being adapted to carry out the control method according to any one of claims 1-10.
12. A refrigerator, comprising:
a case defining a storage compartment therein for storing articles;
the door body is connected with the box body to open and/or close the storage compartment; and
the pesticide residue detection system of claim 11, disposed on the housing or the door.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210406702.4A CN116953265A (en) | 2022-04-18 | 2022-04-18 | Pesticide residue detection system, control method thereof and refrigerator |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210406702.4A CN116953265A (en) | 2022-04-18 | 2022-04-18 | Pesticide residue detection system, control method thereof and refrigerator |
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| CN116953265A true CN116953265A (en) | 2023-10-27 |
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| CN202210406702.4A Pending CN116953265A (en) | 2022-04-18 | 2022-04-18 | Pesticide residue detection system, control method thereof and refrigerator |
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| CN (1) | CN116953265A (en) |
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