CN116926072B - 一种抑制神经干细胞诱导分化方法及其应用 - Google Patents
一种抑制神经干细胞诱导分化方法及其应用 Download PDFInfo
- Publication number
- CN116926072B CN116926072B CN202310490405.7A CN202310490405A CN116926072B CN 116926072 B CN116926072 B CN 116926072B CN 202310490405 A CN202310490405 A CN 202310490405A CN 116926072 B CN116926072 B CN 116926072B
- Authority
- CN
- China
- Prior art keywords
- neural stem
- stem cells
- lncrna
- differentiation
- sirna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000004069 differentiation Effects 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 11
- 210000001178 neural stem cell Anatomy 0.000 title claims description 83
- 230000001105 regulatory effect Effects 0.000 claims abstract description 13
- 230000001276 controlling effect Effects 0.000 claims abstract description 5
- 108020004459 Small interfering RNA Proteins 0.000 claims description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 14
- 230000014509 gene expression Effects 0.000 claims description 11
- 238000001890 transfection Methods 0.000 claims description 9
- 108020004414 DNA Proteins 0.000 claims description 8
- 230000024245 cell differentiation Effects 0.000 claims description 8
- 239000013613 expression plasmid Substances 0.000 claims description 7
- 238000003259 recombinant expression Methods 0.000 claims description 7
- 239000013612 plasmid Substances 0.000 claims description 5
- 230000005764 inhibitory process Effects 0.000 claims description 4
- 125000002091 cationic group Chemical group 0.000 claims description 3
- 238000001638 lipofection Methods 0.000 claims description 3
- 230000001404 mediated effect Effects 0.000 claims description 3
- 239000002773 nucleotide Substances 0.000 claims description 3
- 125000003729 nucleotide group Chemical group 0.000 claims description 3
- 239000012096 transfection reagent Substances 0.000 claims description 3
- 230000030279 gene silencing Effects 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 210000003050 axon Anatomy 0.000 abstract description 14
- 230000006698 induction Effects 0.000 abstract description 3
- 210000005036 nerve Anatomy 0.000 abstract 7
- 210000000130 stem cell Anatomy 0.000 abstract 7
- 210000004027 cell Anatomy 0.000 description 21
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000012634 fragment Substances 0.000 description 11
- 238000011144 upstream manufacturing Methods 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 230000029087 digestion Effects 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 101100460704 Aspergillus sp. (strain MF297-2) notI gene Proteins 0.000 description 2
- 241000193163 Clostridioides difficile Species 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 210000001947 dentate gyrus Anatomy 0.000 description 2
- 239000012154 double-distilled water Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 210000001320 hippocampus Anatomy 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000027089 Parkinsonian disease Diseases 0.000 description 1
- 206010034010 Parkinsonism Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 108091046869 Telomeric non-coding RNA Proteins 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000001130 astrocyte Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000001787 dendrite Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 210000003140 lateral ventricle Anatomy 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 210000001259 mesencephalon Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000001577 neostriatum Anatomy 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000956 olfactory bulb Anatomy 0.000 description 1
- 210000004248 oligodendroglia Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 238000002660 stem cell treatment Methods 0.000 description 1
- 230000002861 ventricular Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0623—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
- C12N2310/141—MicroRNAs, miRNAs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Developmental Biology & Embryology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310490405.7A CN116926072B (zh) | 2023-05-04 | 2023-05-04 | 一种抑制神经干细胞诱导分化方法及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310490405.7A CN116926072B (zh) | 2023-05-04 | 2023-05-04 | 一种抑制神经干细胞诱导分化方法及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN116926072A CN116926072A (zh) | 2023-10-24 |
CN116926072B true CN116926072B (zh) | 2024-03-08 |
Family
ID=88379547
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202310490405.7A Active CN116926072B (zh) | 2023-05-04 | 2023-05-04 | 一种抑制神经干细胞诱导分化方法及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN116926072B (zh) |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105238784A (zh) * | 2015-10-16 | 2016-01-13 | 上海交通大学 | 长非编码rna及在作为调控神经干细胞增殖和分化中的应用 |
WO2016142719A1 (en) * | 2015-03-12 | 2016-09-15 | Genome Research Limited | Biallelic genetic modification |
US9822359B1 (en) * | 2014-01-22 | 2017-11-21 | University Of South Florida | Method of treating neurological disorders using long non-coding RNAs |
CA3064064A1 (en) * | 2017-05-16 | 2018-11-22 | Exostem Biotec Ltd. | Methods of inhibiting aging and treating aging-related disorders |
WO2019191341A1 (en) * | 2018-03-27 | 2019-10-03 | Factor Bioscience Inc. | Nucleic acid-based therapeutics |
CN113122536A (zh) * | 2021-03-31 | 2021-07-16 | 南通大学 | 一种促进神经干细胞向神经元分化的长链非编码rna及其筛选方法 |
WO2021219840A1 (en) * | 2020-04-30 | 2021-11-04 | Secarna Pharmaceuticals Gmbh & Co. Kg | Pd-1-specific antisense oligonucleotide and its use in therapy |
WO2022019832A1 (en) * | 2020-07-20 | 2022-01-27 | Cellresearch Corporation Pte Ltd | A method of generating an induced pluripotent stem cell, an induced pluripotent stem cell and methods of using the induced pluripotent stem cell |
WO2022055239A1 (ko) * | 2020-09-10 | 2022-03-17 | 고려대학교 산학협력단 | 기능성 신경교세포를 단기간에 효율적으로 분화시키는 방법 |
CN114748508A (zh) * | 2022-05-12 | 2022-07-15 | 昆明医科大学第一附属医院 | 一种神经干细胞外泌体及其制备药物中的用途 |
-
2023
- 2023-05-04 CN CN202310490405.7A patent/CN116926072B/zh active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9822359B1 (en) * | 2014-01-22 | 2017-11-21 | University Of South Florida | Method of treating neurological disorders using long non-coding RNAs |
WO2016142719A1 (en) * | 2015-03-12 | 2016-09-15 | Genome Research Limited | Biallelic genetic modification |
CN105238784A (zh) * | 2015-10-16 | 2016-01-13 | 上海交通大学 | 长非编码rna及在作为调控神经干细胞增殖和分化中的应用 |
CA3064064A1 (en) * | 2017-05-16 | 2018-11-22 | Exostem Biotec Ltd. | Methods of inhibiting aging and treating aging-related disorders |
WO2019191341A1 (en) * | 2018-03-27 | 2019-10-03 | Factor Bioscience Inc. | Nucleic acid-based therapeutics |
WO2021219840A1 (en) * | 2020-04-30 | 2021-11-04 | Secarna Pharmaceuticals Gmbh & Co. Kg | Pd-1-specific antisense oligonucleotide and its use in therapy |
WO2022019832A1 (en) * | 2020-07-20 | 2022-01-27 | Cellresearch Corporation Pte Ltd | A method of generating an induced pluripotent stem cell, an induced pluripotent stem cell and methods of using the induced pluripotent stem cell |
WO2022055239A1 (ko) * | 2020-09-10 | 2022-03-17 | 고려대학교 산학협력단 | 기능성 신경교세포를 단기간에 효율적으로 분화시키는 방법 |
CN113122536A (zh) * | 2021-03-31 | 2021-07-16 | 南通大学 | 一种促进神经干细胞向神经元分化的长链非编码rna及其筛选方法 |
CN114748508A (zh) * | 2022-05-12 | 2022-07-15 | 昆明医科大学第一附属医院 | 一种神经干细胞外泌体及其制备药物中的用途 |
Non-Patent Citations (5)
Title |
---|
LncRNA Airsci increases the inflammatory response after spinal cord injury in rats through the nuclear factor kappa B signaling pathway;Tao Zhang;Kang Li;Zi-Lu Zhang;Kai Gao;Chao-Liang Lv;;Neural Regeneration Research(第04期);全文 * |
Tao Zhang ; Kang Li ; Zi-Lu Zhang ; Kai Gao ; Chao-Liang Lv ; .LncRNA Airsci increases the inflammatory response after spinal cord injury in rats through the nuclear factor kappa B signaling pathway.Neural Regeneration Research.2020,(第04期),全文. * |
尚进 ; 孙逸贤 ; 曹秀丽 ; 郭辰峻 ; 张毓飞 ; 韩骅 ; 郑敏化 ; .长链非编码RNA对神经干细胞的调控作用及其机制.神经解剖学杂志.2018,(第06期),全文. * |
神经干细胞定向分化过程中编码基因表达谱分析;张靖;朱丽媛;阴彬;侯琳;彭小忠;;基础医学与临床(第06期);全文 * |
长链非编码RNA对神经干细胞的调控作用及其机制;尚进;孙逸贤;曹秀丽;郭辰峻;张毓飞;韩骅;郑敏化;;神经解剖学杂志(第06期);全文 * |
Also Published As
Publication number | Publication date |
---|---|
CN116926072A (zh) | 2023-10-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2019511240A (ja) | ヌクレアーゼを使用するヒト神経幹細胞のゲノム編集 | |
CN107177591A (zh) | 利用CRISPR技术编辑CCR5基因的sgRNA序列及其用途 | |
US20040234972A1 (en) | Method for identifying and purifying smooth muscle progenitor cells | |
CN106636210B (zh) | 转录因子组合诱导成纤维细胞转分化为类睾丸间质细胞的方法 | |
Huang et al. | miR‐1‐mediated induction of cardiogenesis in mesenchymal stem cells via downregulation of Hes‐1 | |
Nazari et al. | Fibrin hydrogel as a scaffold for differentiation of induced pluripotent stem cells into oligodendrocytes | |
US20200190536A1 (en) | Recombinant lentiviral vector for stem cell-based gene therapy of sickle cell disorder | |
WO2022156378A1 (zh) | 基因转录框架、载体系统、基因组序列编辑方法及应用 | |
CN116926072B (zh) | 一种抑制神经干细胞诱导分化方法及其应用 | |
JP2006517101A (ja) | 治療用産物を送達するための、増殖性の幹細胞および前駆細胞における候補分子の持続的発現 | |
CN111733160A (zh) | 使用CRISPR-Cas9系统对间充质干细胞进行CKIP-1基因敲除的方法 | |
CN117126855A (zh) | 一种构建Igf2bp1基因敲除小鼠胚胎干细胞系的方法 | |
CN110747230B (zh) | 一种促进牛骨骼肌卫星细胞成肌分化的方法 | |
CN111662907A (zh) | 一种敲除诱导多能干细胞nans基因的方法和应用 | |
US11624067B2 (en) | In-vitro induction of adult stem cell expansion and derivation | |
CN116656732A (zh) | 一种基于定点敲入etv2诱导表达系统的内皮细胞分化方法 | |
CN112941106B (zh) | 一种通过foxp1基因编辑和突变延缓间充质干细胞衰老的方法 | |
CN111961650B (zh) | 一种体外获得神经胶质细胞的方法及其应用 | |
JP4188241B2 (ja) | 細胞分化を制御するプロモーター | |
KR102091511B1 (ko) | 줄기세포 분화 효율 촉진 방법 | |
CN115054612B (zh) | 一种可用于促进骨形成的增强子及其应用 | |
CN114958767B (zh) | 基于hiPSC细胞构建的神经干细胞制剂的制备方法 | |
CN117431258B (zh) | 使用含有Tet1基因的重编程因子诱导人体细胞重编程的方法 | |
WO2012156721A1 (en) | Methods for providing human cells comprising a human artificial chromosome | |
CN110623975B (zh) | 调节TERT基因表达的microRNA328及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20240129 Address after: 1319-14, 13th Floor, No. 599 Guangzhou Avenue Middle, Yuexiu District, Guangzhou City, Guangdong Province, 510399 Applicant after: Guangzhou Feilaiai Life Technology Co.,Ltd. Country or region after: China Address before: Unit 602.603, 6th Floor, No. 5 Spiral Fourth Road, International Biological Island, Guangzhou, Guangdong Province, 510000 Applicant before: GUANGZHOU ZHUNYOU BIOLOGICAL TECHNOLOGY Co.,Ltd. Country or region before: China |
|
GR01 | Patent grant | ||
GR01 | Patent grant |