CN116907959A - Equipment for manufacturing cell blocks - Google Patents
Equipment for manufacturing cell blocks Download PDFInfo
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- CN116907959A CN116907959A CN202210696442.9A CN202210696442A CN116907959A CN 116907959 A CN116907959 A CN 116907959A CN 202210696442 A CN202210696442 A CN 202210696442A CN 116907959 A CN116907959 A CN 116907959A
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- cell
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- centrifuge tube
- tube
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 75
- 238000000746 purification Methods 0.000 claims abstract description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 20
- 239000013049 sediment Substances 0.000 claims abstract description 15
- 238000001556 precipitation Methods 0.000 claims abstract description 7
- 230000000249 desinfective effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 82
- 238000005119 centrifugation Methods 0.000 claims description 28
- 239000002699 waste material Substances 0.000 claims description 23
- 238000001035 drying Methods 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 239000008188 pellet Substances 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 14
- 238000002791 soaking Methods 0.000 claims description 14
- 238000005303 weighing Methods 0.000 claims description 14
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000009924 canning Methods 0.000 claims description 11
- 238000011049 filling Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 9
- 239000000645 desinfectant Substances 0.000 claims description 8
- 238000004062 sedimentation Methods 0.000 claims description 7
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- 238000007639 printing Methods 0.000 claims description 4
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2101/00—Chemical composition of materials used in disinfecting, sterilising or deodorising
- A61L2101/32—Organic compounds
- A61L2101/34—Hydroxy compounds
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
Abstract
The application discloses a cell mass production device, which comprises: the cell collection module is used for forming a cell precipitation block at the bottom of the centrifuge tube; the cell block manufacturing module is used for taking out and embedding the cell sediment blocks from the centrifuge tube to manufacture cell blocks; the purification and disinfection module is used for purifying and disinfecting the environment for manufacturing the cell blocks after manufacturing the cell blocks; the intelligent control module is used for controlling the collection of the cell collection module, the manufacture of the cell block manufacturing module and the purification and disinfection of the purification and disinfection module; the intelligent control module controls the collection of the cell collection module, the manufacture of the cell block manufacturing module and the purification and disinfection of the purification and disinfection module, so that the standardization of cell block manufacturing is realized, the intelligent control module can be suitable for manufacturing of micro cell specimens, the quality of cell blocks is improved, the follow-up detection result is improved, the use of manpower is reduced, the cell block manufacturing time is shortened, and the efficiency of cell block manufacturing is improved.
Description
Technical Field
The application belongs to the technical field of medical equipment, and particularly relates to equipment for manufacturing cell blocks.
Background
Medical treatment of body fluid cells is generally carried out by directly coating cells with liquid onto a glass sheet and observing the cells with a microscope. With the development of cytopathology in recent years, the limitations of the common smear made by the conventional technology are more and more obvious, such as uniqueness of the smear and incapability of replication; cannot be permanently preserved; the use of emerging pathology techniques such as immunohistochemistry and molecular pathology techniques is hampered.
The cell mass is prepared by extracting cells in liquid, fixing with paraffin, and making into tissue slice. The method solves the problems of the common smear, and can also avoid doctor-patient disputes caused by smear damage, loss and the like. Meanwhile, the preparation of the cell wax block is also the basis for preparing the tissue chip, and the technology can be widely applied to the field of cytopathology.
At present, the cell wax block is basically prepared by a manual method, and the treated body fluid cells can comprise hydrothorax, ascites, cervical abscission cells, serosal cavity effusion, cerebrospinal fluid, sputum and the like. The brief steps of manual making are as follows: pouring the extracted body fluid into a test tube, centrifuging the test tube at high speed for a plurality of times, and removing supernatant fluid at the upper part of the test tube to form collected liquid cells into cell clusters; the whole cell pellet is carefully peeled off, wrapped with water permeable filter paper, subjected to a series of steps of fixing, dehydrating, transparentizing, staining, waxing, cooling, embedding, and the like, and then the embedded cell pellet is cut into slices of about 5 μm under a microtome, thereby finally completing the cell wax pellet. In the steps, the operation is finished manually, so that the labor is wasted, the time is long, and the formed result is not uniform due to the fact that the operation methods of all people are not uniform, and the subsequent detection effect is affected.
Disclosure of Invention
The application aims to solve the technical problems of overcoming the defects in the prior art and providing the equipment for manufacturing the cell block, wherein the intelligent control module is used for controlling the collection of the cell collection module, the manufacture of the cell block manufacturing module and the purification and disinfection of the purification and disinfection module, so that the purposes of realizing the standardization of manufacturing the cell block, reducing the manpower use, reducing the manufacturing time of the cell block and improving the manufacturing efficiency of the cell block are achieved.
In order to solve the technical problems, the application adopts the basic conception of the technical scheme that:
the application provides a method for manufacturing a cell block, which comprises the following steps:
the cell collection module is used for forming a cell precipitation block at the bottom of the centrifuge tube;
the cell block manufacturing module is used for taking out and embedding the cell sediment blocks from the centrifuge tube to manufacture cell blocks;
the purification and disinfection module is used for purifying and disinfecting the environment for manufacturing the cell blocks after manufacturing the cell blocks;
the intelligent control module is used for controlling the collection of the cell collection module, the manufacture of the cell block manufacturing module and the purification and disinfection of the purification and disinfection module.
Further, the cell collection module includes the liquid feeding module, and the liquid feeding module includes:
the liquid adding tube is used for sequentially adding a fixing solution, a coloring agent and cell gel into the centrifuge tube with the cell sample;
the flow pump is used for providing power for the liquid adding of the liquid adding pipe;
and the weighing device is used for weighing the centrifuge tube after the liquid adding.
Further, the cell collection module further comprises a centrifugation module comprising:
the first centrifugal machine is used for carrying out first centrifugation on the centrifugal tube after the liquid adding;
and the second centrifugal machine is used for carrying out second centrifugation on the centrifugal tube after the first centrifugation, and cell sedimentation blocks are formed at the bottom of the centrifugal tube.
Further, the cell collection module further includes a waste liquid collection module, the waste liquid collection module including:
and the waste liquid collecting tank is used for collecting the supernatant in the centrifugal tube after centrifugation.
Further, the cell mass production module includes a drying module including:
and a drying tube for feeding the dry powder into the centrifuge tube with the cell pellet formed therein and drying the cell pellet.
Further, the cell mass production module further comprises an embedding box and a filling module, and the filling module comprises:
and the canning machine is used for adding the fixing agent into the embedding box.
Further, the cell mass production module further comprises:
a poking needle for poking out the cell-coated sediment block from the opening at the bottom of the centrifuge tube into the embedding box fixing agent for embedding and fixing to prepare a cell block;
and the soaking box is used for soaking the cell blocks in the embedding box to wait for dehydration.
Further, the cell mass production module further comprises:
the code scanner is used for scanning the patient bar code on the centrifuge tube before the cell bag sediment block is poked out of the centrifuge tube;
the embedded box code printer is used for receiving the patient bar code information transmitted by the scanner and printing the patient bar code information on the embedded box.
Further, the decontamination module comprises:
a liquid supply pipe for supplying disinfectant to the environment where the cell mass is produced;
and the sprayer is arranged at the liquid outlet of the liquid supply pipe and is used for spraying disinfectant to the environment for manufacturing the cell mass.
Further, the cell collection module and the cell block manufacturing module comprise a mechanical arm and a conveyor belt which are used for assisting the transfer of the centrifuge tube, and the mechanical arm and the conveyor belt are controlled by the intelligent control module.
By adopting the technical scheme, compared with the prior art, the application has the following beneficial effects.
The intelligent control module controls the collection of the cell collection module, the manufacture of the cell block manufacturing module and the purification and disinfection of the purification and disinfection module, so that the standardization of cell block manufacturing is realized, the intelligent control module can be suitable for manufacturing of micro cell specimens, the quality of cell blocks is improved, the follow-up detection result is improved, the use of manpower is reduced, the cell block manufacturing time is shortened, and the cell block manufacturing quality and efficiency are improved.
The following describes the embodiments of the present application in further detail with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort.
Fig. 1 is a flow chart of an apparatus for cell mass production according to an embodiment of the present application.
Icon: 1-a liquid adding module; 11-a liquid adding pipe; 12-flow pump; 13-a weighing device; 2-a centrifugal module; 21-a first centrifuge; 22-a second centrifuge; 3-a drying module; 4-cell mass preparation module; 41-poking the needle; 42-soaking box; 5-a waste liquid collection module; 51-a waste liquid collection tank; 6-canning module; 61-canning; 7-embedding the box; 8-purifying and sterilizing module.
It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present application, and the following embodiments are used to illustrate the present application, but are not intended to limit the scope of the present application.
In the description of the present application, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.
In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1, the present application provides an apparatus for manufacturing a cell mass, comprising:
the cell collection module is used for forming a cell precipitation block at the bottom of the centrifuge tube;
a cell mass preparation module 4 for taking out and embedding the cell pellet mass from the centrifuge tube to prepare a cell mass;
the purification and disinfection module 8 is used for purifying and disinfecting the environment for manufacturing the cell block after manufacturing the cell block;
the intelligent control module is used for controlling the collection of the cell collection module, the production of the cell block production module 4 and the purification and disinfection of the purification and disinfection module 8.
In the embodiment of the application, the collection of the cell collection module, the preparation of the cell block preparation module 4 and the purification and disinfection of the purification and disinfection module 8 are controlled by the intelligent control module, so that the standardization of the cell block preparation is realized, the method can be suitable for preparing micro cell specimens, the quality of the cell blocks is improved, the manpower is reduced, the preparation time of the cell blocks is shortened, and the efficiency of the cell block preparation is improved.
The cell sample is a body fluid cell, and the body fluid cell comprises hydrothorax, ascites, cervical abscission cells, serosal cavity effusion, cerebrospinal fluid, sputum and the like; the intelligent control module comprises a controller.
Before the centrifuge tube is placed into the equipment inlet, an operator firstly places a cell specimen into a special centrifuge tube, then sticks a patient bar code on the outer side wall of the centrifuge tube, and then places the centrifuge tube into the equipment inlet which is the equipment initial position.
Further, the cell collection module and the cell block manufacturing module both comprise a mechanical arm and a conveyor belt which are used for assisting in transferring the centrifuge tube, the mechanical arm and the conveyor belt are controlled by the intelligent control module, and the cell collection module and the cell block manufacturing module can share one conveyor belt and are used for transferring the centrifuge tube between the cell collection module and the cell block manufacturing module and between devices of each module, so that the centrifuge tube is prevented from being transferred manually;
the cell collection module and the cell block manufacturing module can share a mechanical arm for moving the centrifuge tube to the corresponding position; the controller controls the motion of the conveyor belt and the mechanical arm.
Further, before the cells are placed into the centrifuge tube, the cell specimen is filtered through the filter screen, and interference substances in the cell specimen are filtered, so that the background of the cell slice can be ensured to be clear, and the diagnosis rate is improved; wherein, the interference thing includes blood cell protein, floccule etc. and the filter screen is independent of the centrifuging tube.
Further, after the cell specimen is placed in the special centrifuge tube, a patient bar code is attached to the outer side wall of the centrifuge tube, and patient information is stored in the patient bar code, wherein the patient information specifically comprises the name of the patient, the type of the cell specimen and the like.
As shown in fig. 1, in the embodiment of the present application, the cell collection module includes a filling module 1, and the filling module 1 includes:
a liquid adding tube 11 for sequentially adding a fixing liquid, a coloring agent and a cell gel into the centrifuge tube containing the cell sample;
a flow pump 12 for powering the filling tube;
and the weighing device 13 is used for weighing the centrifuge tube after the liquid adding.
In an embodiment of the present application, the robotic arm controller controls the flow pump 12 to operate the robotic arm;
specifically, the conveyor belt transfers the centrifuge tube from the initial position of the device to the liquid adding tube 11, and the mechanical arm removes the centrifuge tube with the cell specimen from the conveyor belt and places the centrifuge tube on the weighing device 13 for weighing;
the liquid adding pipe 11 adds a certain weight of fixing liquid into the centrifuge tube with the cell specimen under the assistance of the flow pump 12, and fixes the cells in the centrifuge tube;
then adding a certain weight of eosin into the centrifuge tube by the liquid adding tube 11 under the assistance of the flow pump 12, and staining cells in the centrifuge tube;
finally, the liquid adding pipe 11 adds cell gel into the centrifuge tube with the help of the flow pump 12, and the weight of the added cell gel can not be counted for cell coagulation in the centrifuge tube;
finally forming cell suspension in the centrifuge tube, capturing cells, placing the centrifuge tube on a conveyor belt by a mechanical arm, and entering the next working procedure; the final weight of the centrifuge tube is required to reach the set weight, so that preparation is made for the next centrifugation;
the liquid adding pipe 11 for fixing liquid, the liquid adding pipe 11 for coloring agent and the liquid adding pipe 11 for cell gel can be the same liquid adding pipe 11 or can be different liquid adding pipes 11; the weighing device 13 can be a balance, and the weighing device 13 and the liquid adding pipe 11 are integrated at one place;
the formalin and other fixing liquids are used for fixing cells, and the formalin does not damage cell morphology, so that the completion of the cells is ensured, and the cells are shaped; the staining agents such as eosin or Su Gongsu are used for staining cells, so that the accuracy of slicing is improved; cell coacervate such as sodium hyaluronate is used for coacervating cells, capturing and supporting the cells, and can sink together with the cells, so that the cells are directly agglomerated, the cells are better precipitated, and a small amount of cells can be agglomerated; the fixing liquid, the coloring agent and the cell gel can capture cells, the fixing liquid and the cell gel can enable the cells to have good forms, and the coloring agent can enable the cells to have good coloring effects.
As shown in fig. 1, in an embodiment of the present application, the cell collection module further includes a centrifugation module 2, and the centrifugation module 2 includes:
a first centrifuge 21 for centrifuging the centrifuge tube a first time to form a cell pellet at the bottom of the centrifuge tube a first time;
a second centrifuge 22 for subjecting the tube to a second centrifugation to form a cell pellet at the bottom of the tube a second time.
In the embodiment of the application, when centrifuging a centrifuge tube with cell suspension formed, a first centrifuge 21 firstly performs first centrifugation on the centrifuge tube, a second centrifuge 22 performs second centrifugation on the centrifuge tube taken out from the first centrifuge 21, and a complete cell precipitation block is obtained by performing two times of centrifugation on the centrifuge tube;
the controller controls the centrifugal operation of the first centrifuge 21 and the second centrifuge 22;
specifically, during the first centrifugation, centrifuging the centrifuge tube at a centrifugal speed of 1500-2500 rpm for 5-10 minutes, and agglomerating cell bottoms in the centrifuge tube; during the second centrifugation, centrifuging the centrifuge tube at the centrifugal speed of 1500-2500 r for 5-10 min, and shaping the cell sediment in the centrifuge tube; setting the centrifugal speed of the centrifugal tube at the first centrifugal speed and the centrifugal speed of the centrifugal tube at the second centrifugal speed to be the second centrifugal speed, wherein the second centrifugal speed is larger than the first centrifugal speed;
in order to obtain a good centrifugal effect during centrifugation, the centrifugal tubes need to be symmetrically centrifuged, if the number of the centrifugal tubes is singular, the centrifugal tubes need to be weighted into weight bottles with the same mass during centrifugation, for example, the weight of the centrifugal tubes after the liquid is added is 81 grams, and the centrifugal tubes are one, so that the centrifugal tubes need to be weighted into 81 grams automatically when in centrifugation.
As shown in fig. 1, further, the manufacturing apparatus further includes a waste liquid collection module 5, and the waste liquid collection module 5 includes:
a waste liquid collection tank 51 for collecting the supernatant in the centrifuge tube; the waste liquid collecting tank 51 collects the supernatant in the centrifuge tube after the first centrifugation and after the second centrifugation, so that the supernatant is prevented from being discarded randomly and polluting the environment;
specifically, the mechanical arm conveys the centrifuge tube with the cell suspension formed to the first centrifuge 21, the centrifuge tube is placed in the first centrifuge 21, the first centrifuge 21 carries out first centrifugation on the centrifuge tube, the mechanical arm takes out the centrifuge tube after centrifugation from the first centrifuge 21 and transfers the centrifuge tube to the waste liquid collecting tank 51, the mechanical arm pours out the supernatant in the centrifuge tube to the waste liquid collecting tank 51, cell precipitation blocks are formed at the bottom of the centrifuge tube for the first time, the mechanical arm conveys the centrifuge tube to the liquid adding pipe 11, the mechanical arm conveys the centrifuge tube to the corresponding position of the liquid adding pipe 11, the liquid adding pipe 11 adds alcohol or formalin into the centrifuge tube, the mechanical arm conveys the centrifuge tube to the second centrifuge 22, the mechanical arm conveys the centrifuge tube into the second centrifuge 22, the second centrifuge 22 carries out second centrifugation on the centrifuge tube, the mechanical arm takes out the centrifuge tube from the second centrifuge 22 and conveys the centrifuge tube to the waste liquid collecting tank 51, the supernatant in the centrifuge tube is poured into the waste liquid collecting tank 51, and the cell precipitation blocks are formed at the bottom of the second time.
As shown in fig. 1, in the embodiment of the present application, the cell mass production module 4 includes a drying module 3, and the drying module 3 includes:
and the drying tube is used for feeding the dry powder into the centrifuge tube with the cell pellet formed therein, and absorbing water and drying the cell pellet.
In the embodiment of the application, the controller controls the powder adding of the drying pipe and controls the opening and closing of the drying pipe; after the second centrifugation is finished, pouring out the supernatant in the centrifuge tube, and as the supernatant cannot be completely poured out, causing the rest liquid in the centrifuge tube, absorbing water and drying the cell sediment block by adding dry powder into the centrifuge tube with the cell sediment block; the dry powder comprises animal and plant proteins, etc.
The centrifuge tube that the conveyer belt will be formed with cell sediment piece shifts to dry pipe department, and the arm takes off the centrifuging tube from the conveyer belt, places in the corresponding position department of dry pipe, and dry powder is put into to the centrifuging tube to the dry pipe, and after the drying, the arm places the centrifuging tube on the conveyer belt, and the conveyer belt shifts the centrifuging tube to next process.
As shown in fig. 1, in an embodiment of the present application, a cell mass production module includes:
a poking needle 41 for poking out the cell-coated sediment block from the opening at the bottom of the centrifuge tube into the fixing agent of the embedding box 7 for embedding and fixing to prepare a cell block;
a soaking box 43 for formalin-soaking the cell block in the embedding box 7 to wait for dehydration.
In an embodiment of the present application, further, the cell block manufacturing module 4 further includes an embedding cassette 7 and a filling module, and the filling module 6 includes: a filling machine 61 for adding a fixing agent into the embedding cassette 7.
The conveyor belt transfers the embedding box 7 from the initial position of the equipment to the position of the canning machine 61, the mechanical arm removes the embedding box 7 from the conveyor belt and places the embedding box 7 in the canning machine 61, and the canning machine 61 adds a fixing agent into the fixing ring of the embedding box 7;
the centrifuge tube is transferred to the poking needle by the transmission belt, the embedding box 7 is transferred to the poking needle 41, the embedding box 7 and the centrifuge tube are placed on the corresponding positions of the poking needle 41 by the mechanical arm, the poking needle 41 is driven by the mechanical arm, so that the poking needle 41 pokes cell sediment blocks out of the centrifuge tube from top to bottom, the cell sediment blocks are poked by the poking needle 41 from top to bottom, and are poked into the fixing agent in the embedding box 7 through the opening at the bottom of the centrifuge tube under the poking force, and the fixing agent embeds and fixes the cell blocks to prepare the cell blocks; the mechanical arm places the embedding box 7 on a conveyor belt; the conveyor belt transfers the embedding box 7 embedded with the cell blocks to the soaking box 43, the mechanical arm opens the box cover of the embedding box 7 and puts the embedding box 7 into formalin in the soaking box 43 for soaking, and the embedding box 7 waits for dehydration; the fixing agent is agar gel.
After the poking needle 41 pokes the first part of the cell sedimentation block to the first position in the fixing agent from top to bottom, the embedding box 7 filled with the fixing agent moves under the drive of the mechanical arm, the poking needle 41 rotates to change the position under the drive of the mechanical arm while the embedding box 7 moves, the poking needle 41 pokes the second part of the cell sedimentation block to the second position in the fixing agent from top to bottom, and the moving speed of the fixing agent is consistent with the rotating speed of the poking needle 41; the controller controls the motion of the poking needle 41, the motion of the mechanical arm, the operation of the canning machine 61 and the motion of the mechanical arm;
the bottom of the centrifugal tube is provided with an opening with a downward opening, the opening of the bottom of the centrifugal tube is provided with a sealing cover for opening and closing the opening of the bottom of the centrifugal tube, and the sealing cover is in threaded connection with the centrifugal tube; the sealing cover is a screw cap, the screw cap is provided with a first thread, a second thread is arranged on the inner wall or the outer wall of the opening of the centrifuge tube, and the centrifuge tube is in threaded connection with the screw cap through the first thread and the second thread; after the cell sedimentation block is taken out from the centrifuge tube, the centrifuge tube and the screw cap are placed in a consumable collecting box.
The poking needle 41 is a poking needle 41 with a certain extending length, and the poking needle 41 can be arranged on the centrifuge tube or can be arranged independently of the centrifuge tube;
after the scanning is finished, the mechanical arm opens the box cover of the embedding box 7, the nut at the bottom of the centrifuge tube is screwed down in the horizontal direction of the mechanical arm, and the poking needle 41 is vertically aligned with the front end of the fixing ring of the embedding box 7.
The cell mass production module 4 further includes: the device also comprises a code scanner and an embedding box code printer; the scanner is used for scanning the patient bar code of the centrifuge tube and transmitting the acquired patient bar code information to the embedding box code printer; the embedded box code printer is used for printing the bar code information of the patient on the embedded box 7; the consistency of sample and block taking is achieved by printing the bar code information of the patient on the centrifuge tube on the embedding box 7; when the centrifugal tube is scanned, the centrifugal tube is rotated, so that the scanning accuracy is improved; the controller controls the code scanner and the embedded box code printer to work.
As shown in fig. 1, in the embodiment of the present application, the decontamination module 8 includes:
a liquid supply pipe for supplying disinfectant to the environment where the cell mass is produced;
and the sprayer is arranged at the liquid outlet of the liquid supply pipe and is used for spraying disinfectant to the environment for manufacturing the cell mass.
In the embodiment of the application, waste liquid, waste gas, peculiar smell and the like can be generated in the process of manufacturing the cell block, so that the environment for manufacturing the cell block is purified after the cell block is manufactured, and disinfectant can be sprayed into the environment for manufacturing the cell block through a liquid supply pipe and a sprayer to realize the purification of the environment, wherein the disinfectant can be alcohol; for example, after the completion of the production of the cell mass, 75% alcohol is sprayed to the washing waste liquid collecting tank 51 by using a liquid supply pipe and a sprayer for 30 seconds; the controller controls the liquid supply pipe to supply liquid.
The working process of the cell mass production equipment is as follows:
the cell specimen is human body fluid;
filtering the cell sample through a filter screen, and placing the filtered cell sample into a centrifuge tube;
sticking a patient bar code on the outer side wall of the centrifuge tube, and placing the centrifuge tube at the initial position of the equipment;
the centrifuge tube with human body fluid is transferred to the liquid adding tube 11 from the initial position of the device by the conveyor belt, the centrifuge tube is placed on the weighing device 13 by the mechanical arm, the centrifuge tube is firstly weighed by the weighing device 13, the weight of the centrifuge tube is 70 g, 10 g of formalin, 1 g of eosin and transparent sodium alidate are added into the centrifuge tube on the weighing device 13 by the liquid adding tube 11 under the assistance of the flow pump 12, so that the weight of the centrifuge tube reaches 81 g; if the weight of the centrifuge tube is less than 70 g initially, adding formalin of more than 10 g to enable the weight of the centrifuge tube to reach 81 g; eventually a cell suspension is formed in the centrifuge tube,
transferring the centrifugal tube with the cell suspension to a first centrifugal machine 21 by a mechanical arm, transferring the centrifugal tube by the mechanical arm, symmetrically placing the centrifugal tube into the first centrifugal machine 21 at the first centrifugal position, centrifuging the centrifugal tube for the first time by the first centrifugal machine 21 at a centrifugal speed of 2000 revolutions and a centrifugal time of 10 minutes, taking out the centrifuged centrifugal tube from the first centrifugal machine 21 by the mechanical arm, transferring the centrifugal tube to a waste liquid collecting tank 51, pouring the supernatant in the centrifugal tube into the waste liquid collecting tank 51 by the mechanical arm, transferring the centrifugal tube to a liquid adding pipe 11, placing the centrifugal tube at the liquid adding pipe 11, adding 20 milliliters of 95% alcohol into the centrifugal tube, transferring the centrifugal tube to a second centrifugal machine 22 by the mechanical arm, placing the centrifugal tube into the second centrifugal machine 22 by the mechanical arm, centrifuging the centrifugal tube for the second time of 10 minutes at a centrifugal speed of 2500 revolutions, taking out the centrifuged centrifugal tube from the second centrifugal machine 22, transferring the centrifugal tube to the waste liquid collecting tank 51, pouring the supernatant in the centrifugal tube into the waste liquid collecting tank 51, and finally forming a cell sediment block at the bottom of the centrifugal tube;
the mechanical arm transfers the centrifugal tube with the cell pellet to a drying tube, the mechanical arm is arranged at the drying tube, the centrifugal tube is placed at the drying tube, the drying tube is filled with dry powder, and the dry powder absorbs water and dries the cell pellet in the centrifugal tube;
the mechanical arm transfers the embedding box 7 from the initial position of the equipment to the canning machine 61, the mechanical arm transfers the embedding box 7 to the canning machine 61, 4 g of agar gel is added into the embedding box 7 by the canning machine 61, after the filling is finished, the mechanical arm places the embedding box 7 on a conveyor belt, and the conveyor belt transfers the embedding box 7 to a poking needle;
the conveyer belt transfers the embedding box 7 to the poking needle, the conveyer belt transfers the centrifuge tube from the dry position to the poking needle, when the centrifuge tube reaches the poking needle, the mechanical arm integrally rotates the centrifuge tube, and the centrifuge tube rotates 360 degrees, so that the scanner scans the patient bar code on the centrifuge tube, the scanner transfers the patient bar code information to the laser embedding box code printer, and the patient bar code is printed on the embedding box 7;
after the scanning is finished, the mechanical arm opens the box cover of the embedding box 7, the mechanical arm unscrews the nut at the bottom of the centrifuge tube in the horizontal direction, the poking needle 41 is vertically aligned with the front end of the fixed ring of the embedding box 7, the mechanical arm clamps the poking needle 41, the poking needle 41 pokes the cell sedimentation block out of the centrifuge tube from top to bottom, and the cell sedimentation block is poked out into the agarose gel in the embedding box 7 to prepare the cell block; the mechanical arm closes the box cover of the embedding box 7, and places the embedding box 7 on a conveyor belt; the conveyor belt transfers the embedding box 7 to the soaking box 43, the mechanical arm opens the box cover of the embedding box 7, the embedding box 7 is placed in the soaking box 43, and formalin in the soaking box 43 is used for soaking and waiting for dehydration of cell blocks;
after the cell mass was produced, 75% alcohol was sprayed to the washing waste liquid collecting tank 51 by using a liquid supply pipe and a sprayer for 30 seconds.
The foregoing description is only illustrative of the preferred embodiment of the present application, and is not to be construed as limiting the application, but is to be construed as limiting the application to any and all simple modifications, equivalent variations and adaptations of the embodiments described above, which are within the scope of the application, may be made by those skilled in the art without departing from the scope of the application.
Claims (10)
1. An apparatus for producing a cell mass, comprising:
the cell collection module is used for forming a cell precipitation block at the bottom of the centrifuge tube;
the cell block manufacturing module is used for taking out and embedding the cell sediment blocks from the centrifuge tube to manufacture cell blocks;
the purification and disinfection module is used for purifying and disinfecting the environment for manufacturing the cell blocks after manufacturing the cell blocks;
the intelligent control module is used for controlling the collection of the cell collection module, the manufacture of the cell block manufacturing module and the purification and disinfection of the purification and disinfection module.
2. The apparatus for manufacturing cell mass according to claim 1, wherein the cell collection module comprises a liquid adding module comprising:
the liquid adding tube is used for sequentially adding a fixing solution, a coloring agent and cell gel into the centrifuge tube with the cell sample;
the flow pump is used for providing power for the liquid adding of the liquid adding pipe;
and the weighing device is used for weighing the centrifuge tube after the liquid adding.
3. The apparatus for cell mass production of claim 2, wherein the cell collection module further comprises a centrifugation module comprising:
the first centrifugal machine is used for carrying out first centrifugation on the centrifugal tube after the liquid adding;
and the second centrifugal machine is used for carrying out second centrifugation on the centrifugal tube after the first centrifugation, and cell sedimentation blocks are formed at the bottom of the centrifugal tube.
4. The apparatus for manufacturing a cell mass according to claim 3, wherein the cell collection module further comprises a waste liquid collection module, the waste liquid collection module comprising:
and the waste liquid collecting tank is used for collecting the supernatant in the centrifugal tube after centrifugation.
5. The apparatus of any one of claims 1-4, wherein the cell mass production module comprises a drying module comprising:
and the drying tube is used for feeding the dry powder into the centrifuge tube with the cell pellet formed therein, and absorbing water and drying the cell pellet.
6. The apparatus of claim 5, wherein the cell block preparation module further comprises an embedding cassette and a filling module, the filling module comprising:
and the canning machine is used for adding the fixing agent into the embedding box.
7. The apparatus for cell block preparation according to claim 6, wherein the cell block preparation module further comprises:
a poking needle for poking out the cell-coated sediment block from the opening at the bottom of the centrifuge tube into the embedding box fixing agent for embedding and fixing to prepare a cell block;
and the soaking box is used for soaking the cell blocks in the embedding box to wait for dehydration.
8. The apparatus for cell block preparation according to claim 7, wherein the cell block preparation module further comprises:
the code scanner is used for scanning the patient bar code on the centrifuge tube before the cell bag sediment block is poked out of the centrifuge tube;
the embedded box code printer is used for receiving the patient bar code information transmitted by the scanner and printing the patient bar code information on the embedded box.
9. The apparatus for manufacturing cell mass according to claim 8, wherein the decontamination module comprises:
a liquid supply pipe for supplying disinfectant to the environment where the cell mass is produced;
and the sprayer is arranged at the liquid outlet of the liquid supply pipe and is used for spraying disinfectant to the environment for manufacturing the cell mass.
10. The apparatus of any one of claims 1-4, wherein the cell collection module and the cell pellet fabrication module each comprise a robotic arm and a conveyor belt for assisting in transferring the centrifuge tube, the robotic arm and conveyor belt being controlled by the intelligent control module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210696442.9A CN116907959A (en) | 2022-06-20 | 2022-06-20 | Equipment for manufacturing cell blocks |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210696442.9A CN116907959A (en) | 2022-06-20 | 2022-06-20 | Equipment for manufacturing cell blocks |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116907959A true CN116907959A (en) | 2023-10-20 |
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ID=88353604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210696442.9A Pending CN116907959A (en) | 2022-06-20 | 2022-06-20 | Equipment for manufacturing cell blocks |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116907959A (en) |
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2022
- 2022-06-20 CN CN202210696442.9A patent/CN116907959A/en active Pending
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