CN116897937A - Suspending agent containing cyenopyrafen and bifenazate and application thereof - Google Patents

Abstract

The invention provides a cyenopyrafen-bifenazate suspending agent, which comprises the following components in percentage by weight: 10-20% of cyenopyrafen, 25-35% of bifenazate, 1-8% of Agrilan755, 0.5-8% of MORWET-D500, 1-5% of ethylene glycol, 0.1-1% of magnesium aluminum silicate, 0.05-0.5% of xanthan gum, 200.1-0.3% of bactericide BIT, 0.01-1% of defoamer and 100% of water. The suspending agent provided by the invention has excellent preparation performance and biological activity.

Description

Suspending agent containing cyenopyrafen and bifenazate and application thereof

Technical Field

The invention relates to the technical field of pesticides, in particular to a suspending agent containing cyenopyrafen and bifenazate and application thereof.

Background

The hydrolysate of cyenopyrafen metabolized in the organism acts on complex II of the mitochondrial electron transport system, impeding the electron flow from succinic acid to coenzyme Q, thus disturbing the intracellular respiration of the spider mites. Can be used for preventing and treating red spider of apple trees, spider mites of apple trees and spider mites of strawberry.

The phenylhydrazine ester is a novel selective acaricide for foliar spray, is effective for all life stages of mites, has ovicidal activity and knockdown activity on adult mites, and has long lasting period. The product can be used for apple and grape control of apple red spider, tetranychus urticae and McDaniel mites, tetranychus urticae and Lewis mites of ornamental plants, etc.

CN104522010a discloses a synergistic acaricidal composition containing cyenopyrafen and bifenazate, the weight portion ratio of the cyenopyrazate to the bifenazate is 20:1-1:20, and the weight percentage of the total weight of the cyenopyrazate and the bifenazate in the acaricidal composition is 5% -85%. The two active ingredients of the invention have obvious synergistic effect, and have excellent control effect on various mite pests on crops such as fruit trees, vegetables, cotton, tea trees and flowers. The patent does not describe the suspending agent. In addition, pesticide enterprises do not have the product registration related to the tebufenpyrad and bifenazate suspending agent.

The research of pesticide formulation is an important aspect of pesticide development, and a good formulation can play the function of active ingredients as far as possible, can be covered on a target to the greatest extent, reduces the pesticide dosage, facilitates the use of pesticides and reduces the influence on the environment. The company developed a suspending agent formulation with excellent formulation properties and physical activity for the combination of cyenopyrafen and bifenazate.

Disclosure of Invention

The invention provides the following technical scheme:

the tebufenpyrad/bifenazate suspending agent comprises the following components in percentage by weight: 10-20% of cyenopyrafen, 25-35% of bifenazate, 1-8% of Agrilan755, 5000.5-8% of MORWET-D, 1-5% of ethylene glycol, 0.1-1% of magnesium aluminum silicate, 0.05-0.5% of xanthan gum, 20.1-0.3% of bactericide BIT, 0.01-1% of defoamer and 100% of water.

The tebufenpyrad/bifenazate suspending agent comprises the following components in percentage by weight: 15% of cyenopyrafen, 30% of bifenazate, 1-8% of Agrilan755, 1-5% of MORWET-D5000.5, 1-5% of ethylene glycol, 0.1-1% of magnesium aluminum silicate, 0.05-0.5% of xanthan gum, 20.1-0.3% of bactericide BIT, 0.01-1% of defoamer and 100% of water.

The tebufenpyrad/bifenazate suspending agent comprises the following components in percentage by weight: 15% of cyenopyrafen, 30% of bifenazate, 2.5% of Agrilan755, 4.5% of MORWET-D500, 4% of ethylene glycol, 0.5% of magnesium aluminum silicate, 0.12% of xanthan gum, 20.15% of bactericide BIT, 0.05% of defoamer and 100% of water.

The preparation method of the fenpyrad/bifenazate suspending agent comprises the following steps: the preparation method comprises mixing raw materials and adjuvants according to a certain proportion with water as dispersion medium, wet superfine pulverizing, adding zirconia beads into the mixture, grinding at high speed at 1500-2000r/min at room temperature, reducing the average particle size of suspending agent to less than 3 μm, and separating zirconium beads to obtain pesticide suspending agent.

The fentebufenpyrad/bifenazate suspending agent is used for preventing and controlling crop insect pests. Preferably, the insect pest is red spider.

By adopting the technical scheme, the invention has the following beneficial effects:

the bifenazate and the cyenopyrafen have higher toxicity to young citrus mites, and have obvious synergism after being mixed and matched.

When Agrilan755 and MORWET-D500 were combined in the suspension formulation, the suspension was excellent in pourability and substantially free of wall built-up. The 45% bifenazate and etoxazole suspending agent has excellent control effect on controlling red spiders of citrus trees and apple red spiders, and is recommended to dilute 6000-10000 times for the red spiders of the citrus trees; aiming at apple red spider: the preparation is diluted 2500-3500 times.

Detailed Description

All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The invention is further illustrated with reference to specific embodiments.

Example 1: determination of combined toxicity of bifenazate and cyenopyrafen mixture on panonychus citri by leaf-dish spray method and formula screening

1. Purpose of test

The toxicity of the single bifenazate and the cyenopyrafen on the panonychus citri is measured by a leaf disc spraying method, and the combined toxicity of the single bifenazate and the cyenopyrafen on the mixed agents with different proportions is determined, so that the optimal formula of the mixed agents is determined.

2. Materials and methods

2.1 test targets

Test mites: the citrus panonychus citri is collected from green orange base of Qing dynasty, qing county, henan province in 1 ten days of 2021 respectively, and is kept in a cage with citrus seedlings indoors, and newly hatched citrus panonychus citri female adult mites are picked up as acaricide indoor virulence measurement test mites.

2.2 instrumentation

BURKARD type automatic control spray tower, precision pipette gun, electronic balance (0.1 mg), pipettor, beaker, measuring cylinder, incubator, etc.

2.3 pharmaceutical Agents

98% of bifenazate raw medicine; 97.3% of cyenopyrafen original drug.

2.4 dose setting

Table 1 test dose setting of bifenazate and cyenopyrafen and their blends against panonychus citri

2.5 test time

The test time is 2021, 7 months, 15 days to 7 months and 19 days, and the test mites are young mites of panonychus citri.

2.6 test method

The test uses leaf disk spraying method, and refers to the indoor biological assay guidelines of acaricide NY/T1154.13-2008 and 1154.7-2006.

The test agent is diluted with clean water according to geometric progression to prepare 5 gradient concentrations, and the indoor toxicity measurement is carried out by adopting a leaf disk spray method. Selecting citrus leaves with consistent growth, making leaf discs by using a puncher, placing a wet sponge block in a culture dish, placing filter paper on the wet sponge block, placing leaf discs on the filter paper, inoculating 20 female mites on each leaf disc, performing stem leaf spraying treatment by using a BURKARD type automatic control spraying tower, placing the culture dish in a Potter spraying tower chassis for spraying, taking out after the liquid medicine is settled for 1min, then placing the culture dish in an insect breeding room with the temperature of (25+/-1), the relative humidity of 70% -80 and the illumination period of L:D=16:8, and performing treatment by using 0.01% Triton-80 solution as a control, wherein each treatment is performed for 1 culture dish, and repeating for 4 times. Mortality was checked 48h after treatment and mite body darkening inactivity was used as a citrus panonychus mortality standard.

3 data processing and statistics method

3.1 mortality, correction mortality calculation method

Mortality and corrected mortality were calculated in percent (%) based on the number of dead mites and total mites in the treated and control groups.

Wherein:

p1-mortality;

k-represents the number of dead mites;

n-represents the total mites treated

Wherein:

p2-correct mortality;

pt-treatment mortality;

p0-blank mortality.

3.2LC50 calculation method

The data was analyzed using a probability value analysis system using a DPS processing system.

3.3 method for calculating co-toxicity coefficient

Referring to the co-toxicity coefficient method of grand Yunpei, a single dose of bifenazate is used as a standard control medicament, and the toxicity index is 100.

Toxicity index of standard acaricide a (bifenazate): 100

Toxicity index of Standard acaricide B (cyenopyrafen)

Theoretical toxicity index of mixed agent tti=a agent toxicity index x a content (%) +b agent toxicity index x B content (%)

If the co-toxicity coefficient CTC of the mixture is greater than 120, the synergistic effect is shown; CTCs below 80 indicating antagonism; CTCs between 80-120 exhibit additive effects.

4 results and evaluation

Table 2 results of indoor toxicity measurements of bifenazate and cyenopyrafen on panonychus citri

The results show that the bifenazate and the cyenopyrazate have higher toxicity to the female adult mites of the panonychus citri, the LC50 of the bifenazate is 8.83mg/L, the LC50 of the cyenopyrazate raw medicine is 2.19mg/L, and the bifenazate: cyenopyrafen (mass ratio after content conversion) 8: 1. 4: 1. 2: 1. 1:1 and 1: LC50 of the 2 proportion mixed medicament to the panonychus citri is respectively 4.80, 2.95, 2.59, 2.28 and 2.08mg/L, and the co-toxicity coefficients CTC are 137.61, 186.34, 169.56, 153.93 and 128.18 respectively.

In conclusion, the bifenazate and the cyenopyrafen have higher toxicity to citrus whole-claw young mites, and the combined toxicity after mixing has obvious synergism, and the bifenazate is used as the active ingredients in five proportions: the common toxicity coefficient of the cyenopyrafen is higher than that of the cyenopyrafen in the range of 4:1 and 2:1. The bifenazate and the cyenopyrafen are mixed and are expected to become an effective medicament for controlling the citrus red strains in the field.

Example 2: bifenazate and cyenopyrafen suspending agent formula

TABLE 3 formulation composition of suspending agents

Remarks:

agrilan 788: hydrophobic group modified carboxylic acid copolymer from Nanjing Jieron;

agrilan 755: hydrophilic mono-comb polymers; from Nanjing Jierou;

Morwet-D500: a mixture of alkyl naphthalene sulfonate formaldehyde polycondensate and block copolymer; from Nanjing Jierou;

the preparation method of the suspending agent comprises the following steps:

mixing raw materials and auxiliary materials according to a certain proportion by taking water as a dispersion medium, carrying out wet superfine grinding, adding zirconia beads into the mixture during grinding, grinding at a high speed of 1500-2000r/min at room temperature, reducing the average particle size of the suspending agent to be less than 3 mu m, and obtaining the pesticide suspending agent after separating the zirconia beads.

Testing the performance indexes of the preparation:

when Agrilan 788 was used alone or in combination with Agrilan 788 and MORWET-D500, the suspending agent was less pourable and wall built-up was likely to occur. When Agrilan755 and MORWET-D500 were combined, the suspending agent was excellent in pourability and substantially free from wall built-up. Formulas 1-4 meet the control index of the suspending agent, and formula 5 is a disqualified preparation. In the field test, the field control effect test is carried out by adopting the formulas 1-4.

TABLE 4 suspension formulation Performance index

Example 3: test report of 45% bifenazate and etoxazole suspending agent for controlling red spider of citrus trees

Test purpose: and verifying the effect of the test agent on controlling the red spiders of the citrus trees and the safety of the citrus trees, and providing a field pesticide effect basis for registering the agent.

1. Test conditions

Crop and target: crop/citrus tree/golden block

Target/red spider (Panoneichus citri)

Test conditions: the test was carried out in Peng Shanou Fengming town civilian 7, mitsui, sichuan, 24 th month in 2020 to 5 th month in 24 th month. Citrus tree variety: gold blocks, 4 multiplied by 4m are unpacked, the tree age is 4 years, fertilizer is applied for 2 to 3 times per year, the compound fertilizer is about 100 g/plant/time, the flowering phase of citrus and the occurrence phase of red spiders. The field is sandy loam, the pH value is 6.8, the organic matter content is 1.70%, and the fertility level is moderate.

2. Test design and arrangement

Test agent

45% Bifenazate/etoxazole suspension (Shanghai Yue Ling Biotechnology Co., ltd.)

Cell area and repetition: and repeating for 4 times, wherein each cell is provided with 2 trees, and a protection row is arranged among the cells.

Time and frequency of application: spraying for 1 time in the flowering phase and the red spider emergence phase of the citrus trees on 24 days of 4 months in 2020.

Using the apparatus and the application method: the liquid medicine is prepared by adopting a secondary dilution method, and the main preparation tool comprises the following steps: balance, cylinder, pipette, glass stick, etc. Spraying with HD400 sprayer with spraying pressure of 0.2-0.4Mpa and spray hole diameter of 1mm. And the whole plant is uniformly sprayed, and the water consumption of each plant is about 2 liters based on the condition that the whole plant leaves of the citrus trees are fully wetted.

Weather data: 24 days of 4 months in 2020, raining in early morning, sunny when the medicine is applied, and no influence on the test is caused.

Soil data: sandy loam, pH value of 6.8, organic matter content of 1.70%, fertility level of the sandy loam, etc.

Control of non-target biological conditions: 20% gibberellic acid soluble powder is applied 7000 times to preserve fruits on 5 months and 8 days 2020.

3 investigation and results

3.1 investigation method: the tender shoots are marked in the east, west, south, north and middle 5 directions of the tree in each cell, the quantity of insects on 5 leaves is investigated for each shoot, and the quantity of movable mites on 25 leaves is investigated in total. And directly observing leaf surfaces by using a handheld magnifier, and counting the number of mites.

3.2 investigation time and times: the pre-drug and post-drug were investigated 1 time each for 1, 10, 15, 20, 30 days, 6 times in total. The specific time is as follows: 24 days in 2020, 25 days in 2020, 4 days in 2020, 9 days in 2020, 14 days in 2020, 24 days in 2020.

3.3 the test complies with the "quality management Specification for pesticide registration test", GB/T17980.11-2000, SOP-TM-074a "orange Red spider test", the calculation formula is as follows

3.4 effects on other organisms: no significant effect of the test agent on other organisms was observed during the test.

3.5 test results

45% bifenazate and etoxazole suspending agent for preventing and controlling red spiders of citrus trees, and recommended dilution is 6000-10000 times, and spraying is carried out for 1 time in the occurrence period of the red spiders of the citrus trees

Table 5: test for controlling red spider of citrus tree by using 45% bifenazate and etoxazole suspending agent

Example 4: the field efficacy test purpose of the 45% bifenazate-cyenopyrafen suspending agent for preventing and controlling apple tree red spiders is as follows: and verifying the effect of the test agent on controlling the red spiders of the citrus trees and the safety of the citrus trees, and providing a field pesticide effect basis for registering the agent.

1. Test conditions

The test is carried out on an apple demonstration garden which is a modern agricultural technology innovation base in Tianjin city, which is arranged on east Pu Wa street in Wuqing district in Tianjin city. Apple, pear, cherry, peach, etc. are planted in the garden and are integrated.

Test target conditions

The red spider and the hawthorn red spider Tetranychus viennensis Zacher can activate dynamic mites. The mite leaves can see 6.5-8.5 heads of young mites, if mites, adult mites and other various movable mites during the test.

Test crop, growth period and growth condition

The test apple tree is an adult tree with the age of about 8-10 years, the plant and row spacing is 2.5m multiplied by 3m, the plant height is more than 2.5m, and the diameter of the canopy is about 1.8m.

Soil type of test field

A slightly alkaline clay loam having a pH of about 7.8; the organic matter content is 1.2%.

Water and fertilizer management for test lands

Conventional water and fertilizer management, irrigation and drainage strips in the garden

Meteorological data

The current day of application (2022, 06, 23 days) has small and sporadic rain, southern wind, wind power of 1-2 levels, the highest temperature of 35.9 ℃, the lowest temperature of 22.7 ℃ and the average temperature of 28.6 ℃; in the middle period of the test (short-term gusts of rain occur in the 5d and 6d days after the medicine, the rain amount is 3.3mm and 6.5 mm), and the total weather is fine.

Medicament data for preventing and treating other diseases and insect pests

No other insecticide and acaricide are sprayed on apple trees in each treatment area 15 days before and during the test

2. Test design and arrangement

Test agent

45% Bifenazate/etoxazole suspension (Shanghai Yue Ling Biotechnology Co., ltd.)

Cell area and repetition

Cell area: 4 plants; 2 protection trees or isolation trees are additionally arranged between the cells so as to prevent liquid medicine drift crosstalk. Number of repetitions: four times, the inter-cell random block arrangement.

Pesticide application method

Time and method of administration

The medicine is sprayed by using a backpack type manual sprayer at 2022 and 06 and 23 days, and the blank control area is sprayed with equal amount of clean water.

Applicator instrument: supergreen is not comparable to 16-type manual sprayers.

The use capacity is as follows: the liquid medicine amount of a single plant is 800-1000 ml, the liquid medicine amount is adjusted according to the size of the tree crown, and in principle, the leaves are suitable for dripping water.

3. Investigation and results

Investigation time and number: the live dynamic mites of the apple trees in each treatment area are investigated on the 2 nd day, the 7 th day and the 14 th day before the application. The total number of checks was 4.

The investigation method comprises the following steps: selecting middle 2 test trees in each cell, fixing according to branches in the east, west, south, north and middle five directions, listing, investigating the number of active mites on 10 leaves, checking and recording the number of active mites on the leaves by using a handheld magnifying glass.

The drug effect calculation method comprises the following steps: and performing variance analysis on the control effect data of each cell by using a DMRT method, and comparing the significance of the difference between the average control effects of the medicament treatment by using an SSR method. The control effect (%) was calculated according to the following respective methods.

CK0: pre-drug mite base number for blank control zone, CK1: number of mites after the blank control area is treated;

PT0: pre-drug mite port base number in drug treatment zone, PT1: number of mites after the treatment of the area with the agent

4. Direct impact on crops

No adverse effect of the test agent on apple tree growth vigor, branches, fruit surface and the like is found.

5. Impact on other organisms

No adverse effect of the test agent on the non-target organisms was observed in the investigation.

Test results

In the initial period of living dynamic mites of apple trees, the suspension agent preparation of 45% bifenazate and cyenopyrafen is diluted 2500-3500 times, and the suspension agent preparation is uniformly sprayed for 1 time inside and outside the bore of the apple trees and on the upper and lower parts of the canopy, so that the front and the back of the leaves are required to be uniformly subjected to medicine, and the medicine liquid amount is suitable for dripping water from the leaves, so that the harm of the apple tree red mites can be effectively controlled.

Table 6: field efficacy test of 45% bifenazate-cyenopyrafen suspending agent for preventing and controlling apple tree red spiders

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same.

Claims (6)

1. The tebufenpyrad/bifenazate suspending agent is characterized by comprising the following components in percentage by weight: 10-20% of cyenopyrafen, 25-35% of bifenazate, 1-8% of Agrilan755, 0.5-8% of MORWET-D500, 1-5% of ethylene glycol, 0.1-1% of magnesium aluminum silicate, 0.05-0.5% of xanthan gum, 20.1-0.3% of bactericide BIT, 0.01-1% of defoamer and 100% of water.

2. The tebufenpyrad/bifenazate suspension concentrate of claim 1, wherein tebufenpyrad 15%, bifenazate 30%, agrilan755 1-8%, MORWET-D500.5-8%, ethylene glycol 1-5%, magnesium aluminum silicate 0.1-1%, xanthan gum 0.05-0.5%, bactericide BIT 20.1-0.3%, defoamer 0.01-1%, water make up 100%.

3. The tebufenpyrad/bifenazate suspension concentrate of claim 1, wherein tebufenpyrad 15%, bifenazate 30%, agrilan755 2.5%, MORWET-D500.5%, ethylene glycol 4%, magnesium aluminum silicate 0.5%, xanthan gum 0.12%, bactericide BIT 20.15%, defoamer 0.05%, water make up 100%.

4. The tebufenpyrad/bifenazate suspension concentrate according to claim 1, 2 or 3, characterized in that the preparation method comprises the following steps: the preparation method comprises mixing raw materials and adjuvants according to a certain proportion with water as dispersion medium, wet superfine pulverizing, adding zirconia beads into the mixture, grinding at high speed at 1500-2000r/min at room temperature, reducing the average particle size of suspending agent to less than 3 μm, and separating zirconium beads to obtain pesticide suspending agent.

5. Use of the tebufenpyrad-bifenazate suspension concentrate according to any one of claims 1-4 for controlling crop pests.

6. The use according to claim 5, wherein the pest is a red spider.