CN115336584A - Application of plant growth regulator containing diethyl aminoethyl hexanoate in regulating ginger growth - Google Patents
Application of plant growth regulator containing diethyl aminoethyl hexanoate in regulating ginger growth Download PDFInfo
- Publication number
- CN115336584A CN115336584A CN202211153711.3A CN202211153711A CN115336584A CN 115336584 A CN115336584 A CN 115336584A CN 202211153711 A CN202211153711 A CN 202211153711A CN 115336584 A CN115336584 A CN 115336584A
- Authority
- CN
- China
- Prior art keywords
- ginger
- diethyl aminoethyl
- aminoethyl hexanoate
- choline chloride
- plant growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000234314 Zingiber Species 0.000 title claims abstract description 95
- 235000006886 Zingiber officinale Nutrition 0.000 title claims abstract description 89
- 235000008397 ginger Nutrition 0.000 title claims abstract description 89
- OORIKNJWZHTXDC-UHFFFAOYSA-N CCCC(CC)C(CC)(C(=O)OCC)N Chemical compound CCCC(CC)C(CC)(C(=O)OCC)N OORIKNJWZHTXDC-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 230000012010 growth Effects 0.000 title claims abstract description 30
- 239000005648 plant growth regulator Substances 0.000 title claims abstract description 28
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 12
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims abstract description 46
- 229960003178 choline chloride Drugs 0.000 claims abstract description 46
- 235000019743 Choline chloride Nutrition 0.000 claims abstract description 45
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims abstract description 45
- 238000000034 method Methods 0.000 claims description 27
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- 241000196324 Embryophyta Species 0.000 abstract description 33
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
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- 239000010452 phosphate Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
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- 229920001296 polysiloxane Polymers 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical group [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- AFTOVGIFYVNBID-UHFFFAOYSA-M sodium;2-butylnaphthalene-1-sulfonate Chemical compound [Na+].C1=CC=CC2=C(S([O-])(=O)=O)C(CCCC)=CC=C21 AFTOVGIFYVNBID-UHFFFAOYSA-M 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to application of a plant growth regulator containing diethyl aminoethyl hexanoate in regulating the growth of ginger, wherein the effective active ingredients of the plant growth regulator protected by the invention are diethyl aminoethyl hexanoate and choline chloride, and the weight ratio of the diethyl aminoethyl hexanoate to the choline chloride is 2: 19. Experiments prove that the plant growth regulator protected by the invention has good synergistic effect on regulating the growth of the ginger, is applied once in the three-crotch period (3 seedlings) of the ginger and is applied once every 10-15 days, has a regulating effect on the growth of the ginger, is mainly characterized by improving the plant height, the stem thickness and the branch number in the field, can also obviously increase the yield of the ginger, has an improving effect on the quality of the ginger and has no adverse effect on other non-target organisms. The plant growth regulator of the invention has the advantages of slight toxicity, environmental protection, high efficiency, long lasting period and economic cost reduction.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to application of a plant growth regulator containing diethyl aminoethyl hexanoate to regulation of ginger growth.
Background
Diethyl aminoethyl hexanoate is a high-energy plant growth regulator with broad-spectrum and breakthrough effect discovered by American scientists in the 90 s, and has a chemical formula of C 12 H 25 NO 2 . The choline chloride can improve the activities of plant peroxidase and nitrate reductase, improve the content of chlorophyll, accelerate the photosynthetic speed, promote the division and the extension of plant cells, promote the development of root systems and adjust the balance of nutrients in vivo.
Choline chloride (Choline chloride) with molecular formula C 5 H 14 ClNO, molecular weight 139.625,it is mainly used as feed additive in pesticide field, can be absorbed by plant stem, leaf and root, and then quickly transferred to the acting part, and its physiological action can inhibit C 3 The light absorption of the plant promotes the development of root system, and the photosynthetic products can be accumulated in tubers and root tubers as much as possible, thereby increasing the yield and improving the quality.
The growth of ginger is mainly divided into four stages: the ginger has the characteristics in the germination period, the seedling period, the vigorous growth period and the rhizome dormancy period, and the ginger is a temperature-favored crop, so that the environmental and temperature requirements are strict, and the yield and the quality of the ginger are greatly influenced by fertilizer and water management, temperature management, plant immunity and the like in the ginger growth process. At present, main diseases and pests of ginger are ginger blast, borer and grub, the mainly used prevention and control mode is pesticide root irrigation and spraying, and because underground diseases and pests are difficult to prevent and control, farmers often use some highly toxic pesticides in the use process or mix fertilizers, pesticides and plant regulators together for use, so that the purpose of reducing the application times is achieved.
How to provide a plant growth regulator product with good mixing compatibility, high efficiency, environmental protection, low toxicity and long lasting period, and the product which reduces pesticide residue and is safe for eaters under the condition of not reducing prevention effect is a technical problem which is urgently needed to be solved by the technical personnel.
At present, few mixed products which can be used for regulating the growth of ginger in a legal and standard way are available in the market, and 6 products mainly comprise 18% choline chloride-naphthylacetic acid wettable powder, 20% choline chloride-naphthylacetic acid aqueous solution, 8% naphthylacetic acid-ethephon soluble solution and the like.
The applicant compounds diethyl aminoethyl hexanoate and choline chloride to serve as a unique product and a formula for regulating the growth of ginger, can effectively promote the photosynthesis of crop leaves, promote the development of root systems, and enable the photosynthetic products to be accumulated in tubers and root tubers as much as possible, thereby achieving the purposes of increasing the yield and improving the quality. Can also enhance the immunity of crops, prevent the premature senility of stems and leaves and reduce the probability of the infection of germs on the crops, thereby achieving the aim of disease resistance.
Disclosure of Invention
The invention aims to provide an application method of a plant growth regulator for regulating and controlling the growth of ginger, which solves the problems of proper application type, application concentration and application period of the plant growth regulator in ginger production, and also solves the core problems of yield increase of ginger, quality improvement of ginger, environmental protection, safety and low toxicity.
The technical scheme of the invention is as follows:
use of a plant growth regulator comprising diethyl aminoethyl hexanoate for regulating the growth of ginger, wherein: the effective active components of the plant growth regulator are diethyl aminoethyl hexanoate and choline chloride, wherein the weight ratio of diethyl aminoethyl hexanoate to choline chloride is 2: 19.
Further, the total weight of the diethyl aminoethyl hexanoate and the choline chloride in the plant growth regulator is 5-63%, the preferred total weight is 10-42%, and the best total weight is 21%.
Furthermore, the plant growth regulator consists of diethyl aminoethyl hexanoate, choline chloride, a synergist and an auxiliary agent and is prepared into wettable powder.
The synergist is selected from one of polyethoxy modified silane, polyethoxy modified fatty alcohol mixture or alkoxy modified polytrisiloxane.
Further, the plant growth regulator is uniformly sprayed according to the dosage of the effective components of 126-315 g/hectare, and is applied once at the three-crotch stage of ginger and applied once again at intervals of 10-15 days; the preferred dosage of the active ingredient is 157.5-189 g/ha for uniform spraying.
Furthermore, the water consumption of the plant growth regulator is 450-600 liters per hectare.
Compared with the prior art, the invention has the following advantages:
(1) Compared with the prior art, the composition has good synergistic effect, and is obviously superior to a single control agent and a blank control;
(2) The preparation is environment-friendly, slightly toxic, safe to the environment, pollution-free, safe to producers, transporters and users;
(3) The content and photosynthetic rate of chlorophyll, protein and nucleic acid in the plant body are improved, the activity of peroxidase and nitrate reductase is improved, the carbon and nitrogen metabolism of the plant is promoted, the absorption of the plant to water and fertilizer and the accumulation of dry matters are enhanced, and the drought resistance and cold resistance are improved;
(4) The immunity of crops can be enhanced, the disease resistance and insect resistance of the crops can be improved, and the aging of plants can be delayed;
(5) The compatibility is good, and the pesticide can be used together with other pesticides such as bactericides, insecticides and fertilizers, so that the control effect of other pesticides is not influenced;
(6) Can promote crop metabolism, can be used as an antidote, has good repairing effect on plant injury caused by overhigh use concentration of pesticide, and can reduce the residual quantity of the pesticide on crops.
Detailed Description
The first application example is implemented: combined toxicity measurement in ginger growth room by blending and regulating diethyl aminoethyl hexanoate and choline chloride
Purpose of the test
Diethyl aminoethyl hexanoate and choline chloride are used as test medicaments, ginger at the three-crotch stage (Lujiang No. 1) is used as a test object, the regulation effect of the two medicaments and different ratios thereof on the growth of the ginger is measured by adopting a stem leaf spraying method, the optimal ratio is screened, and the test result is reported as follows.
2 conditions of the test
2.1 test target
Ginger (variety, kaempferia galangal 1) is potted indoors and grows to the three-crotch stage.
2.2 culture conditions
The potting method is adopted. A plastic pot with the thickness of 450mm multiplied by 350mm is placed in an enamel tray, the surface soil (the soil loading amount is 4/5) which is not applied with the pesticide and is collected from a farmland, dried and sieved is collected, and then water is directly added from the top of the pot, so that the soil humidity is controlled to be 60-70% at the initial stage. Selecting full and uniform ginger seeds, uniformly placing the ginger seeds on the soil surface, covering soil for 4cm, maintaining soil humidity by adopting a bottom water adding mode after sowing, placing the ginger seeds in an artificial climate chamber with controllable illumination, temperature and humidity for cultivation, and selecting the ginger with consistent growth as a test material when the ginger grows to a three-branch-fork period to carry out a medicament treatment test.
2.3 instrumentation
Electronic balance, pipettor, volumetric flask, beaker, small-sized quantitative sprayer, plastic bowl (450 mm × 350 mm), climatic chamber, etc.
Design of experiment
3.1 reagents
3.1.1 test Agents
98% diethyl aminoethyl hexanoate;
a choline chloride technical with the concentration of 98 percent.
3.2 test treatment
3.2.1 dose setting
The test medicament diethyl aminoethyl hexanoate and choline chloride raw drug are prepared into 1.0 multiplied by 104mg/L mother liquor by taking sterile water as a solvent for standby.
Referring to the respective action activities of the two agents and combining the characteristics of indoor determination, on the basis of a pre-test experiment, the diethyl aminoethyl hexanoate and the choline chloride are set to different mixed combinations by using 0.1% tween 80 aqueous solution, and 35 groups are designed in total according to the following table.
Indoor bioassay was performed using a 0.1% tween 80 aqueous solution containing no drug as a blank.
Table 1 experimental agent dosage design
3.2.2 test replicates
Each treatment was 4 replicates, each replicate treatment was 20 pots, 1 ginger per pot.
3.3 modes of treatment
The test was performed 2 times in total. 1 ginger in each pot is treated with the medicament when the ginger grows to the three-crotch stage, and the medicament treatment is carried out again at intervals of 10 days.
4 test method
4.1 test protocol
The combined action determination of stem leaf spraying method and mixing of plant growth promotion/inhibition test (NY/T2061.2-2011, NY/T2061.5-2016) is determined by referring to the regulation of plant growth regulator in pesticide indoor bioassay test standard
4.2 treatment with pharmaceutical Agents
The test is that the medicine is applied at the three-crotch stage of the ginger, the second time of medicine application is performed at intervals of 10 days, and the liquid medicine is uniformly attached to the leaves of the whole plant and does not drop. Spray treatments were performed in the order of the low dose to the high dose as designed, with an aqueous solution containing 0.1% tween 80 as a control.
5 data investigation and statistical analysis
5.1 results investigation time and methods
And (5) after 60d of the second medicament treatment, randomly selecting 10 pots every time by using an absolute value (number measurement) survey method, and surveying the branch number and the single plant yield of the ginger (the mother ginger is picked off, and only the new ginger is reserved).
5.2 statistical analysis of data
According to the measurement results, the increase rate of the branch number of the ginger and the increase rate of the weight of the single tuber of different treatments are calculated. Calculating theoretical growth rate E of each treatment mixed combination by using Gowing method 0 (E 0 = X + Y-X Y/l 00), and then the combined effect of the two on ginger is evaluated in comparison with the observed growth rate (E).
When the E-Eo value is more than 10 percent, the synergistic effect is achieved, when the E-Eo value is less than-10 percent, the antagonistic effect is achieved, and when the E-Eo value is between-10 percent and 10 percent, the additive effect is achieved. And determining the optimal proportion according to the actual pod number growth rate, the characteristics of the two plant growth regulators, the balance of the formula and other factors.
Wherein X is the branch number growth rate and the single-plant tuber weight growth rate when the dosage of diethyl aminoethyl hexanoate is P; y is the growth rate of the number of branches and the growth rate of the weight of the single tuber when the dosage of choline chloride is Q. The results are shown in Table 2.
6 results and analysis
TABLE 2 Combined effect of different combinations of choline chloride and diethyl aminoethyl hexanoate on ginger growth (Gowing method)
Test results show that diethyl aminoethyl hexanoate and choline chloride have good increasing effect on the branch number of ginger and the weight average of tubers of single plants. The weight increasing rate E-E0 value of each processed ginger single-plant tuber is a positive value when the diethyl aminoethyl hexanoate and the choline chloride are used together, and the drug effect shows different increasing trends. Wherein the proportion of diethyl aminoethyl hexanoate to choline chloride is 2 (20 mg/kg +190 mg/kg), the weight increase rate E-E0 value of a single ginger tuber is 10.75%, and the treatment is relatively maximum in each treatment and shows a synergistic effect.
Application example two: formulation examples
The invention will be further illustrated with reference to the following examples, in which the percentages are by weight, but the invention is not limited thereto.
EXAMPLE 1 wettable powder of 21% diethyl aminoethyl hexanoate and choline chloride
The diethyl aminoethyl hexanoate wettable powder is prepared from 2% of diethyl aminoethyl hexanoate, 19% of choline chloride, 3% of alkoxy modified polytrisiloxane, 3% of alkyl naphthalene sulfonate formaldehyde polymer, 2% of polycarboxylate, 4% of butyl naphthalene sodium sulfonate, 0.3% of anhydrous citric acid, 0.15% of organic silicone, 5% of white carbon black and the balance of calcined kaolin.
EXAMPLE 2 wettable powder of 21% diethyl aminoethyl hexanoate and choline chloride
The content of the diethyl aminoethyl hexanoate in the wettable powder is 2%, the content of the choline chloride in the diethyl aminoethyl hexanoate in the wettable powder is 19%, the content of the polyethoxy modified silane in the mixture of polyethoxy modified fatty alcohol is 4%, the content of alkyl naphthalene sulfonate is 5%, the content of sodium dodecyl sulfate is 4%, the content of potassium dihydrogen phosphate is 1%, the content of fatty alcohol is 0.2%, the content of ammonium sulfate is 20%, and the balance is diatomite, so that the 21% diethyl aminoethyl hexanoate-choline chloride wettable powder is prepared.
EXAMPLE 3 10.5% wettable powder of diethyl aminoethyl hexanoate and choline chloride
The content of the diethyl aminoethyl hexanoate in the wettable powder is 1%, the content of the choline chloride in the diethyl aminoethyl hexanoate in the wettable powder is 9.5%, the content of polyethoxy modified silane in the mixture of polyethoxy modified fatty alcohol is 5%, the methyl naphthalene sulfonate formaldehyde condensate is 3%, the polycarboxylate is 2%, the alpha-sodium alkenyl sulfonate is 2.5%, the fatty acid sulfate acetate is 1.5%, the anhydrous citric acid is 0.5%, the fatty acid is 0.15%, the white carbon black is 8%, and the balance is potassium sulfate.
EXAMPLE 4 wettable powder of 42% diethyl aminoethyl hexanoate and choline chloride
The wettable powder of the diethyl aminoethyl hexanoate and the choline chloride is prepared from 4 percent of diethyl aminoethyl hexanoate, 38 percent of choline chloride, 4 percent of alkoxy modified polytriesiloxane, 3 percent of lignosulfonate, 1 percent of methyl naphthalenesulfonate formaldehyde condensate, 4 percent of butyl naphthalenesulfonic acid sodium salt, 1 percent of alkylbenzene sulfonate, 0.3 percent of monopotassium phosphate, 10 percent of talcum powder and the balance of calcined kaolin.
EXAMPLE 5 wettable powder of 63% diethyl aminoethyl hexanoate and choline chloride
The diethyl aminoethyl hexanoate wettable powder is prepared from diethyl aminoethyl hexanoate with the percent of total percent of 6 percent, choline chloride with the percent of total percent of 57 percent, alkoxy modified polytrisiloxane 4 percent, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate 4 percent, polyoxyethylene polyoxypropylene ether block copolymer 3 percent, styryl phenol formaldehyde resin propylene oxide block polyether 3 percent, alkyl glycoside 4 percent, glacial acetic acid 0.5 percent, white carbon black 8 percent and ammonium sulfate with the balance.
Application example three: evaluation test of safety of 21% wettable Aminopentyl-Choline chloride powder (example 1-2) to ginger
1.1 purpose of the test
The field efficacy evaluation of the 21% choline chloride-diethyl aminoethyl hexanoate wettable powder on the safety of ginger is carried out.
1.2 test time
Test start time: 7 month and 3 days 2019
Test completion time: 18 days in 2019, 10 months
2 test conditions
2.1 selection of test subjects, crops and varieties
Test subjects: safety feature
Test crops and varieties: rhizoma Zingiberis recens/rhizoma Zingiberis recens, rhizoma Zingiberis recens
3 design of the experiment
3.1 dosage and treatment number
Table 3 experimental dose setting
3.2 methods of use
Spray, 450L/hectare
3.3 application time and frequency
The medicine is applied once (7 months and 3 days in 2019) at the three-crotch (3-seedling) stage of ginger, and is applied once again at intervals of 10-15 days (7 months and 14 days) for 2 times.
4.2 investigation methods, time and frequency
4.2.1 investigation time and number of surveys
3,7 days after the application, a phytotoxicity (negative effect) investigation was conducted. Recording the influence of the medicament on wild organisms and beneficial insects in the test area and the influence on other pests and diseases, and visually inspecting whether the plants in each medicament treatment area have phytotoxicity symptoms such as wilting, withered leaves, malformation, yellowing, color spots and the like. The yield of the plot was measured at the time of ginger harvest (18 days in 10 months), 10 ginger plants per plot were randomly picked to record plant height, stem thickness and branch number, and the individual plant yield was determined.
4.2.2 methods of investigation
Plant height: measuring with a tape;
stem thickness: a vernier caliper;
yield per plant: weighing by a balance;
cell yield: weighing by a platform scale.
The obtained results are the average values of all treatments, SPSS statistical software is used for analysis, and the yield results are statistically analyzed by a Duncan's New Complex polar error method (DMRT).
4.2.2 method for calculating drug Effect
The test data were statistically analyzed with the software SPSS 19.
5 results and analysis
TABLE 4 effect of 21% choline chloride diethyl aminoethyl hexanoate wettable powder on ginger safety
TABLE 5 influence of wettable powder of 21% choline chloride-diethyl aminoethyl hexanoate on ginger yield
The test result shows that after the spray treatment of the 21 percent choline chloride diethyl aminoethyl hexanoate wettable powder at the effective component dosage of 189 to 756 g/hectare, the growth of the white ginger, the red claw ginger and the Sichuan rhizoma bambusae is not inhibited, and no phytotoxicity is generated.
Application example four: experiment for measuring influence of 21% diethyl aminoethyl hexanoate-choline chloride wettable powder (examples 1 and 2) on environment
I entrusted third party company to carry out 10 tests such as bee acute oral toxicity test, bee acute contact toxicity test, bird acute oral toxicity test, fish acute toxicity test, silkworm acute toxicity test, daphnia acute activity inhibition test, algae growth inhibition test, earthworm acute toxicity test, natural enemy trichogramma acute toxicity test, natural enemy ladybug acute contact toxicity test and the like on examples 1-2, and the test results are as follows:
1) The bee acute oral toxicity test shows that the toxicity level is micro toxicity.
2) Acute contact toxicity test of bee shows that the toxicity grade is micro toxicity.
3) Acute oral toxicity test of birds shows that the toxicity level is micro-toxicity.
4) Acute toxicity test of fish shows that the toxicity level is micro toxicity.
5) The silkworm acute toxicity test shows that the toxicity level is low.
6) And 5, performing an inhibition test on the daphnia acute activity, wherein the toxicity level is micro-toxicity.
7) The toxicity grade is micro toxicity in the algae growth inhibition test.
8) The earthworm is subjected to acute toxicity test, and the toxicity level is slight toxicity.
9) And (3) the natural enemy trichogramma acute toxicity test is low in risk.
10 Natural enemy ladybug acute contact toxicity test, low risk.
Application example five: examples 1-2 acute toxicology assay experiments
I entrusted a third-party detection institution to carry out acute toxicity determination tests on the samples 1-2, and the test results are as follows:
1) The toxicity grade of the acute oral toxicity test of rats is slight toxicity.
2) Acute percutaneous toxicity test of rats shows that the toxicity grade is slight toxicity.
3) The rabbit eye irritation test is non-irritating.
4) Skin irritation test, no irritation.
5) Skin sensitization test. Experimental animals, guinea pig; the sensitization intensity is zero.
Application example six: 21% diethyl aminoethyl hexanoate-choline chloride wettable powder (example 1) for field efficacy experiment of ginger growth regulation
Purpose of the experiment
The 21% diethyl aminoethyl hexanoate-choline chloride wettable powder (example 1) is verified to evaluate the pesticide effect of ginger growth regulation in the field, and reasonable field use concentration, period and use method are screened in the test, so that a basis is provided for registration of the product.
2 materials and methods
2.1 treatment with Experimental Agents
TABLE 6 treatment of test Agents
2.2 test subjects and crops
Test object growth regulation.
The test crop is ginger, and the variety is Sichuan bamboo root ginger.
2.3 test fields and cultivation conditions
The test address is the copper beam area in Chongqing city.
2.4 treatment method
Cell area: 20 square meters; the number of repetitions: 4.
the solution was sprayed uniformly at a rate of 450L per hectare.
2.5 application time and method
Phytotoxicity (negative impact) was investigated 3,7 days after each application. Recording the influence of the medicament on wild organisms and beneficial insects in the test area and the influence on other pests and diseases, and visually inspecting whether the ginger plants in each medicament treatment area have phytotoxicity symptoms such as wilting, withered leaves, malformation, yellowing and color spots.
At the time of ginger harvest (10 months and 20 days), 5 ginger plants per cell were randomly extracted, the plant height, stem thickness and branch number were recorded, and the individual plant yield and the cell yield were measured. After harvesting, the quality of indexes such as soluble protein, crude fiber and soluble sugar of ginger are measured in 21 days in 10 months.
2.6 methods of investigation
Yield: platform balance weighing
Coarse fiber: acid-washing method
Soluble sugar: anthrone process
Soluble protein: coomassie brilliant blue colorimetric method
The obtained results are the average values of all treatments, SPSS statistical software is used for analysis, and the yield results are statistically analyzed by a Duncan's New Complex polar error method (DMRT).
2.7 methods of calculation and analysis
3 results and analysis
TABLE 7 influence of wettable powder of 21% diethyl aminoethyl hexanoate and choline chloride on ginger growth
TABLE 8 influence of wettable pulvis of diethyl aminoethyl hexanoate and choline chloride on yield increasing effect of ginger
TABLE 9 influence of wettable pulvis of diethyl aminoethyl hexanoate and choline chloride on ginger quality
The test result shows that:
the influence of the treatment of the reagent on the growth amount and yield of the ginger is shown in tables 7 and 8, the plant height, stem thickness and branch number of the ginger are increased along with the increase of the dosage of the reagent, and the plant height, stem thickness and branch number of each treatment are obviously higher than those of a clear water control; compared with the contrast medicament, the plant height and the stem thickness of each treatment are obviously higher than those of the control medicament except that the plant height and the stem thickness of the treatment with the effective component dosage of 126 g/hectare are equivalent to those of the treatment with the contrast medicament, and the branch number of the treatment with the effective component dosage of 189 g/hectare and 315 g/hectare is obviously higher than that of the control medicament. The yield of the ginger after the treatment of the test agent is increased along with the increase of the dosage of the test agent, the cell yield is respectively 62.67kg, 65.03kg, 68.42kg and 69.11kg, and the yield of the four treatments is obviously higher than that of the clear water control; compared with the control agent, the yield of the plot treated by the active ingredient dosage of 126 g/hectare is equivalent to that of the control agent, and the three groups of treatments are obviously higher than that of the control agent. The 4 groups of treatments have promotion effect on the growth of the ginger and can also obviously increase the yield of the ginger, the yield increases are respectively 11.13%, 15.32%, 21.32% and 22.55%, and the yield increases of the other three groups of treatments are obviously higher than those of the control medicament except that the yield increases of the 126 g/ha treatment and the control medicament are equivalent.
The effect of the test agents on ginger quality is shown in table 9. The quality of the ginger can be improved by the test agent as a whole, and compared with a clear water control group, the soluble protein, the crude fiber and the soluble sugar of each treatment are superior to those of the clear water control group, which shows that the four concentrations of the test agent have an effect of improving three indexes of the soluble protein, the crude fiber and the soluble sugar of the ginger.
Application example seven: 21% diethyl aminoethyl hexanoate-choline chloride wettable powder (example 2) field pharmacodynamic experiment for regulating ginger growth
The concrete experiment purpose, experiment basis, experiment medicament, dosage and calculation method refer to the sixth application example.
1 experimental crop:
ginger, the variety is white ginger;
the experimental site: chongqing city Fengdu county
2 application time and frequency
The experiment is applied for 2 times, and the ginger is in the three-crotch (3-seedling) stage (6-month and 22-day 2021) when the first application is performed. The second application is given after 15 days (7 months and 7 days 2021).
3 capacity of use
450 liters of water is used per hectare.
4 investigation time and number of times
And (4) phytotoxicity investigation: observing whether the ginger has phytotoxicity or not 3,7 and 10 days after each pesticide application.
At the time of harvest (10 months and 6 days), the height, stem thickness and branch number of ginger plants are investigated, the yield of each plant and the yield of a cell are measured, and the yield increase rate is calculated.
Quality measurement of rhizoma Zingiberis recens protein content, crude fiber and soluble sugar is carried out at 7 days in 10 months to 8 days in 10 months.
During the experiment, the effect of the test agent on other non-target organisms was observed.
5 investigation method
Plant height, stem thickness, number of branches: sampling is carried out at five points in each cell, 2 ginger plants are randomly selected at each point, and the plant height, stem thickness and branch number are investigated.
Yield per plant: sampling at five points in each cell, randomly selecting 2 ginger plants at each point, and weighing the rhizome of each plant.
Yield: all gingers in each plot were harvested and weighed with balance.
Quality: randomly selecting 25 ginger blocks with uniform size in each cell, dividing each 5 ginger blocks into 1 part, preparing a mixed sample, measuring the protein content by a Coomassie brilliant blue method, measuring the crude fiber content by an acid-base digestion method, and measuring the soluble sugar content by an anthrone method.
5 results of the experiment
TABLE 10 influence of wettable pulvis of diethyl aminoethyl hexanoate and choline chloride on ginger agronomic traits
TABLE 11 influence of wettable powder of 21% diethyl aminoethyl hexanoate and choline chloride on ginger production
TABLE 12 influence of wettable powder of diethyl aminoethyl hexanoate and choline chloride on ginger quality
The test result shows that:
the effect of the 21% wettable amijust ester-choline chloride powder on the agronomic traits of ginger is shown in table 10. The results show that the plant height, stem thickness and branch number of the ginger after the medicament treatment are all obviously higher than those of the clear water control, wherein the plant height, stem thickness and branch number of the effective components of the test medicament which are used in 157.5-315 g/hectare are all obviously higher than those of the two control medicaments.
The effect of the treatment with the agent on ginger production is shown in table 11. The results show that the yield of the ginger after the medicament treatment is greatly improved. After the four concentrations of the test agent are treated, the yield increase rate is 8.39%, 12.39%, 20.91% and 21.64% compared with the yield increase rate of a clear water control. Wherein the dosage of the effective components of the test agent is 157.5-315 g/ha, and the yield per plant, the cell yield and the yield increase are all remarkably higher than those of two control agents.
The effect of the drug treatment on ginger quality is shown in table 12. After the medicament treatment, the contents of protein, vitamin C and crude fiber of the ginger have no significant difference compared with the clear water control, which indicates that the spraying of the medicament has no adverse effect on the quality of the ginger.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Claims (8)
1. Use of a plant growth regulator comprising diethyl aminoethyl hexanoate for regulating the growth of ginger, wherein: the effective active components of the plant growth regulator are diethyl aminoethyl hexanoate and choline chloride, wherein the weight ratio of diethyl aminoethyl hexanoate to choline chloride is 2: 19.
2. Use according to claim 1, characterized in that: the total weight of the diethyl aminoethyl hexanoate and the choline chloride in the plant growth regulator is 5-63%.
3. Use according to claim 2, characterized in that: the total weight of the diethyl aminoethyl hexanoate and the choline chloride in the plant growth regulator is 10-42%.
4. Use according to claim 3, characterized in that: the total weight of diethyl aminoethyl hexanoate and choline chloride in the plant growth regulator is 21 percent.
5. Use according to any one of claims 1 to 4, characterized in that: the plant growth regulator consists of diethyl aminoethyl hexanoate, choline chloride, synergist and assistant and is prepared into wettable powder.
6. Use according to claim 5, characterized in that: the plant growth regulator is uniformly sprayed according to the dosage of the effective components of 126-315 g/hectare, and is applied once at three-crotch of ginger and again at intervals of 10-15 days.
7. Use according to claim 6, characterized in that: the plant growth regulator is evenly sprayed according to the dosage of the effective components of 157.5-189 g/hectare.
8. The method of application according to claim 6, characterized in that: the water consumption of the plant growth regulator is 450 to 600 liters per hectare.
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