CN115281195B - Plant growth regulating composition for regulating tobacco growth - Google Patents
Plant growth regulating composition for regulating tobacco growth Download PDFInfo
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- CN115281195B CN115281195B CN202210431164.4A CN202210431164A CN115281195B CN 115281195 B CN115281195 B CN 115281195B CN 202210431164 A CN202210431164 A CN 202210431164A CN 115281195 B CN115281195 B CN 115281195B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
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Abstract
The invention relates to a plant growth regulating composition for regulating tobacco growth, in particular to a plant growth regulating composition for regulating tobacco growth by brassinolide and anti-sprouting pellet, which is mainly characterized in that: the effective active ingredients of the composition are 24-epibrassinolide and anti-sprouting pellet, and auxiliary agents and other excipients are added to prepare a solution. The composition can obviously regulate the yield increase and bud inhibition of tobacco, can also improve the quality of tobacco, increases income of tobacco farmers, has obviously better regulating effect than that of a single control agent, has low dosage and little residue, and does not find that the composition has phytotoxicity on tobacco stems and leaves in the application range.
Description
Technical Field
The invention belongs to the technical field of pesticides, and in particular relates to a plant growth regulating composition for regulating tobacco growth, which contains brassinolide, anti-sprouting pellets, an auxiliary agent and other excipients.
Background
Bud inhibiting pill (Maleic hydrate), alias Maleic hydrazide, and green fresh element with molecular formula of C 4 H 4 N 2 O 2 Is a butene dihydrazide plant growth regulator. The bud-suppressing pellet is absorbed by leaves, twigs, buds and roots of plants and then is transmitted to the parts of the plants where growth is active through xylem and phloem for accumulation. The mechanism of action is to inhibit cell division of meristematic tissue in the active site of growth.
Brassinolide, an English common name, is also 24-surface, 24-mixed surface, 28-high, 28-surface high, 14-hydroxy brassinosteroids and the like, is a novel green environment-friendly plant growth regulator, and is treated by seed soaking and stem and leaf spraying of brassinolide with proper concentration. Can promote the growth of crops such as vegetables, melons, fruits and the like, improve the quality, increase the yield, have bright color and thicker leaves. Also can lead the leaf picking time of the tea to be advanced, can lead the sugar content of melons and fruits to be higher, the individuals to be larger, the yield to be higher and the storage to be more durable.
With the development of international specialized production technology of tobacco and the continuous improvement of living standard of people, the requirements on the internal quality of tobacco are higher and higher, and the use of efficient and safe chemical bud inhibitor is one of important measures for improving the yield and quality of tobacco. Practice proves that the chemical bud inhibitor can save a great deal of labor, increase the yield, increase the medium and high smoke proportion and increase the income of tobacco farmers.
Disclosure of Invention
The invention aims to provide a plant growth regulating composition for regulating tobacco growth, which has a very good synergistic effect on tobacco bud inhibition and yield increase according to a certain proportion and usage amount.
The technical scheme of the invention is as follows:
a plant growth regulating composition for regulating tobacco growth, characterized by: the effective active ingredients of the composition are brassinolide and the anti-sprouting pellet, and auxiliary agents and other excipients are added to prepare a soluble agent, wherein the weight ratio of the brassinolide to the anti-sprouting pellet is 1:29999.
The total weight of brassinolide and the anti-sprouting pellet in the composition is 9-51%, and the preferred total weight is 30%.
The auxiliary agent and excipient are selected from one or more of wetting agent, salifying agent, solvent, defoamer and PH regulator.
The dosage of the active ingredients of the composition is 900-2250 g/hectare.
The wetting agent may be: one or more of fatty alcohol polyoxyethylene ether, alkyl glycoside, sodium fatty alcohol polyoxyethylene ether sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate, naphthalene sulfonate formaldehyde condensate, p-methoxy fatty amide benzenesulfonic acid, lignin sulfonate, polycarboxylate, alkylphenol polyoxyethylene ether sulfate, calcium alkylbenzenesulfonate, fatty acid ester sulfate, alkylphenol polyoxyethylene ether, polyethylene glycol stearate, sorbitol oleate, sodium dibutylnaphthalene sulfonate, and castor oil polyoxyethylene ether;
the salifying agent can be as follows: one or more of isopropylamine, ethanolamine and triethanolamine;
the solvent may be: one or more of ethanol, isopropanol, diethylene glycol, sec-butyl acetate, dimethyl sulfoxide, ethylene glycol, glycerol, N-methylpyrrolidone and water;
the defoamer can be one or more selected from organic silicone, fatty alcohol, fatty acid and polyether;
the PH regulator can be one or more selected from glacial acetic acid, citric acid, triethanolamine, light calcium carbonate and potassium dihydrogen phosphate;
the composition of the invention comprises the following components in percentage by weight when being prepared into a soluble agent: 0.0003 to 0.0017 percent of brassinolide, 8.9997 to 50.9983 percent of bud inhibition pellet, 1 to 20 percent of wetting agent, 1 to 20 percent of salifying agent, 1 to 40 percent of solvent, 0 to 4 percent of PH regulator, 0 to 3 percent of defoaming agent and the balance of water.
The invention has the advantages that: (1) Compared with a single agent, the composition has good synergistic effect on increasing the yield and improving the quality of tobacco; (2) Labor and cost are saved, the plant is applied once in the recommended period, and buds are not required to be smeared in the whole growing season; (3) The quick and long-acting type pesticide is quick to absorb, the effect is not affected by rainfall after the pesticide is applied for 8 hours, and the pesticide is convenient to apply in rainy seasons; (4) Convenient use, good safety, low toxicity to human and livestock, no residue, and safety to tobacco.
Detailed Description
The invention is further illustrated by the following examples, which are by weight, but are not limited thereto.
Application example one
EXAMPLE 1 30% brassinolide-anti-bud pellet solution
0.001% of brassinolide, 29.999% of anti-sprouting pellet, 5% of fatty alcohol polyoxyethylene ether, 13% of ethanolamine, 15% of sec-butyl acetate, 0.2% of organic silicone and the balance of water.
EXAMPLE 2 30% 24-Epilolide-anti-bud pellet solution
According to the weight percentage, the 24-epibrassinolide is 0.001%, the anti-sprouting pellet is 29.999%, the alkyl glycoside is 8%, the triethanolamine is 20%, the dimethyl sulfoxide is 13%, the polyether defoamer is 0.3%, and the balance is water.
EXAMPLE 3 30% 24-Epilolide-anti-bud pellet solution
According to the weight percentage, the 24-epibrassinolide is 0.001%, the anti-sprouting pellet is 29.999%, the fatty alcohol polyoxyethylene ether sodium sulfonate is 3%, the alkyl glycoside is 3%, the isopropylamine is 14%, the glycerol is 5%, the dimethyl sulfoxide is 8%, the sec-butyl acetate is 6%, the triethanolamine is 0.2%, the polyether defoamer is 0.5%, and the rest is the ethylene glycol.
EXAMPLE 4 30% 24-Epilolide-anti-bud pellet solution
According to the weight percentage, the 24-epibrassinolide is 0.001%, the prosaposin is 29.999%, the alkylphenol polyoxyethylene ether formaldehyde condensate is 2%, the castor oil polyoxyethylene ether is 3%, the ethanolamine is 10%, the isopropanol is 7%, the diethylene glycol is 10%, the fatty alcohol is 0.5%, and the balance is water.
EXAMPLE 5 9% brassinolide-anti-bud pellet solution
According to the weight percentage, the 24-epibrassinolide is 0.0003 percent, the sprouting inhibitor is 8.9997 percent, the alkyl glycoside is 3 percent, the fatty alcohol polyoxyethylene ether is 4 percent, the triethanolamine is 1 percent, the isopropylamine is 8 percent, the sec-butyl acetate is 5 percent, the dimethyl sulfoxide is 6 percent, the fatty acid is 0.4 percent, and the balance is water.
EXAMPLE 6 51% brassinolide-anti-bud pellet solution
According to the weight percentage, the 24-epibrassinolide is 0.0017 percent, the prosaposin 50.9983 percent, the alkylphenol polyoxyethylene ether formaldehyde condensate is 5 percent, the isopropylamine 3, the ethanolamine is 5 percent, the lignosulfonate is 5 percent, the diethylene glycol is 10 percent, the ethanol is 8 percent, the organic silicone is 1 percent, the anhydrous citric acid is 0.5 percent, and the balance is water.
Implementation example II:
combined toxicity test report in tobacco growth chamber regulated by combination of brassinolide and anti-sprouting pellet
Test target: tobacco is "Zhongyan 100".
The test method comprises the following steps: stem and leaf spraying method
Test basis is that reference is made to NY/T2061.2-2011 and NY/T2061.5-2016
1 purpose of test
The brassinolide and the anti-bud pellet are used as test agents, potted tobacco (when the first flower is open, the top leaf is topped more than 20cm, and the axillary bud is topped more than 2 cm) is used as a test object, a spray method is adopted for treatment, the regulating effect of the two agents and different proportions thereof on tobacco growth is measured, the optimal proportions are screened, and an effective agent for regulating tobacco growth is developed for reasonable blending of the two agents, so that a formula screening basis is provided. The test results are reported below.
2 culture conditions
Potted tobacco, the variety of which is Zhongyan 100, is selected to have the first central flower of the tobacco plant open, the top leaf of which is larger than 20cm, and the top-cut plant is used as a test object.
3 test design
3.1 reagents
99.6% of the anti-sprouting pellet original drug provided by the applicant;
90% 24-epibrassinolide prodrug, provided by applicant;
solvent: DMF (dimethyl formamide)
Emulsifying agent: tween 80 (0.1% aqueous solution).
3.2 test treatments
3.2.1 dose setting
Because the two plant growth regulators have complementarity to the regulation effect of tobacco, the respective activity characteristics, the field dosage and the indoor measurement characteristics are combined, and the dosages of 24-epibrassinolide and the anti-sprouting pellet are respectively set as different mixed combinations, as shown in Table 1. The biological assay was performed in each room using water containing no drug, the same solvent and the same emulsifier as a blank.
Table 1 design of experimental dosage
3.2.2 test replicates
The treatment of the test agent was divided into 4 replicates for each concentration, and an aqueous solution containing 0.1% tween 80 was used as a control. 10 pots of tobacco (1 plant/pot) were treated each in duplicate.
4 data investigation and statistical analysis
4.1 time and method of outcome investigation
The number of axillary buds and fresh weight of axillary buds of each repeated 10 tobacco plants with growth more than 2cm are investigated 40 days after the medicine, and the bud inhibition rate and the bud inhibition effect are calculated. After the tobacco leaves reach the harvesting period, harvesting from bottom to top in batches, respectively harvesting all 10 repeated tobacco leaves, naturally airing, respectively weighing each tobacco leaf, and calculating the yield and the yield.
4.2 statistical analysis of data
Referring to the method recommended by NY/T2061.5-2016, the theoretical inhibition (growth rate) E0 (E0=X+Y-X. Times.Y/100) of each treatment mixture combination is calculated by using the Gowing method to calculate each treatment mixture combination, and then the combined effect of the two mixture combinations on tobacco bud inhibition regulation is evaluated compared with the actual yield increase and bud inhibition effect.
When the E-E0 value is more than 10%, the E-E0 value is less than-10%, the antagonism is realized, and the E-E0 value is the addition effect between-10% and 10%. And determining the optimal ratio according to the characteristics of the actual yield increase and the bud inhibition effect on the two plant growth regulators, the balance of the formula and other factors.
Wherein X is the yield increase and bud inhibition effect when the dosage of 24-epibrassinolide is P; y is the yield increase and bud inhibition effect when the dosage of the bud inhibition pill is Q.
5 results and analysis
Table 2-Combined action effects of 24-Epilobium lactone and anti-bud pellet and their different ratios for regulating tobacco growth
As can be seen from the results in Table 2, the actual yields of the test agent 24-epibrassinolide 0.02074mg/L, 0.03111mg/L, 0.04667mg/L, 0.07mg/L and 0.105mg/L treatments were 4.49%, 5.86%, 7.16%, 8.14% and 9.66%, respectively, and the actual yields of the test agent imazalil 1242.56mg/L, 1615.33mg/L, 2099.93mg/L, 2729.91mg/L and 3548.88mg/L treatments were 4.27%, 6.50%, 8.16%, 9.95% and 11.26%, respectively. After the two components are mixed, the combined action type is evaluated by a Gowing method, and the E-E0 values treated when the 24-epibrassinolide and the anti-sprouting pellet are 0.07mg/L and 2099.93mg/L are both more than 10, and the E-E0 is 10.78, so that the synergistic effect is achieved. Therefore, when the ratio of the two is 1:29999, the tobacco yield is well regulated.
The experimental process observation shows that the 24-epibrassinolide and the anti-sprouting agent developed by the applicant has higher activity on increasing the yield and inhibiting sprouting of tobacco when the processing ratio of the preparation is 1:29999, has a certain synergistic effect, is a reasonable ratio of two agents and the treatment of each ratio dose thereof, is safe for the growth of experimental tobacco, and has no phytotoxicity.
Application example three: efficacy experiment of 30% brassinolide-anti-bud pellet solution (example 1) in field for regulating tobacco growth
1 purpose of experiment
The 30% brassinolide and anti-sprouting pellet soluble solution (example 1) developed by the applicant is verified to provide basis for registration of the product, such as regulating effect on tobacco growth, medication adaptation period, recommended use dosage and safety on tobacco growth.
2 materials and methods
The application method comprises the following steps: spraying method, 600 liters of water per hectare.
Test development time: 2021, 6-2021, 8
2.1 Experimental agent treatment
TABLE 3 treatment with reagents
2.2 test subjects and crops
The test object is tobacco, the variety is 'little gold 1025', and the growth vigor is good after transplanting.
2.3 methods of administering drugs
Time of application: applying the pesticide when the central flowers of the first flowers of most tobacco plants are open and the top leaves are larger than 20cm in 14 days of 6 months; manual bud picking treatment is carried out for 1 time on each of 14 days of 6 months, 14 days of 29 months and 14 days of 7 months.
2.4 investigation method
Investigation time: the number of axillary buds was investigated 1 time each of 15d (day 29 of 6 months), 30d (day 14 of 7 months) and 40d (day 24 of 7 months) after the administration, and the fresh weight of axillary buds was investigated at 40d after the administration.
Number of surveys: 3 times.
2.5 calculation and analysis methods
The inhibition rate, the bud inhibition effect and the yield increase are calculated according to the formula (1), the formula (2) and the formula (3), respectively.
Bud ratio (%) = (number of axillary buds of no-bud-picking control region-number of axillary buds of treatment region)/number of axillary buds of no-bud-picking control region×100 … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … (1)
Bud inhibiting effect (%) = (fresh weight of axillary bud in bud-free control area-fresh weight of axillary bud in treated area)/fresh weight of axillary bud in bud-free control area×100 … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … (2)
Yield increase (%) = (treatment area yield-no-shoot control area yield)/no-shoot control area yield×100 … (3)
3 results and analysis
Statistical analysis was performed on the experimental data using the Duncan New complex polar Difference (DMRT) method.
TABLE 4 influence of 30% brassinolide-desmodium-inhibiting pellet soluble on tobacco yield and quality
Conclusion 4
When the first central flowers of most tobacco plants in the tobacco field are open, topping is carried out when the top leaves are larger than 20cm, axillary buds larger than 2cm are beaten off, and 30% brassinolide-bud inhibition pellet solution is uniformly sprayed on the middle part and the front face of the tobacco plants, so that the growth of tobacco can be obviously regulated, the yield of tobacco leaves is improved, and adverse effects on tobacco leaf quality indexes such as nicotine content, potassium-chlorine ratio and Shi Muke value are avoided. Therefore, the 30% brassinolide and anti-sprouting pellet solution is suitable for popularization and application in tobacco production.
Application example four: 30% 24-Epilolide-anti-bud pellet solution (example 2) for tobacco growth promotion experiment report
1 purpose of experiment
The field efficacy evaluation of the 30% 24-epibrassinolide and anti-sprouting pellet soluble agent provided by the applicant on the efficacy of tobacco growth regulation is carried out, and reasonable field use concentration, period and use method are screened through test and screening, so that a basis is provided for registering the product.
2 materials and methods
2.1 experimental crops: tobacco (variety: yunyan 87)
2.2 Experimental agent treatment
TABLE 5 treatment with reagents
3 method of administering a drug
3.1 methods of use
The foliar spray, the water quantity per hectare is 450L.
3.2 times and times of application
The application was carried out 1 time on day 2021, 5 and 15 of the topping day of tobacco, the axillary buds longer than 2cm were scraped off on the topping day, and the manual bud-scraping treatment was carried out 1 time each 10 days and 20 days after the application, and 2 times were taken together.
4 investigation method, time and times
4.1 investigation time and times
Bud inhibition efficacy investigation time: 1 each of 15 days (2021, 5, 30 days), 30 days (2021, 6, 14 days) and 40 days (2021, 6, 24 days) after administration was investigated, 3 times in total.
4.2 investigation method
Taking 3 points in each district 15 days, 30 days and 40 days after the medicine, surveying 10 plants in each fixed mark, recording the number of axillary buds exceeding 2cm length, and calculating the bud inhibition rate. And when investigation is carried out 40 days after the medicine, picking and weighing 30 tobacco axillary buds fixedly marked in each district, and calculating the bud inhibition effect. And (4) harvesting and baking tobacco leaves in time after the tobacco leaves are ripe, accumulating the flue-cured tobacco yield of each batch of all cells in each cell, counting the total yield of the flue-cured tobacco in each cell, calculating the yield increase, and performing analysis of variance. 10 middle flue-cured tobacco leaves are randomly collected in each district for quality analysis, nicotine content, water-soluble total sugar content and protein content are measured, and Shi Muke value (ratio of water-soluble total sugar content to protein content) is calculated.
Leaf size investigation of tobacco leaves: when the tobacco leaves at the lower part, the middle part and the upper part are picked, 10 tobacco leaves at the corresponding picking positions are randomly selected in each district to measure the length and the width of the tobacco leaves.
Single leaf weight investigation: and after the lower, middle and upper tobacco leaves are picked and cured, 50 tobacco leaves corresponding to the picked positions are randomly selected in each district, the total weight of the cured tobacco is weighed, and the single leaf weight of the cured tobacco is measured by calculating an average value.
4.3 method for calculating efficacy
5 experimental results
TABLE 6 influence of 30% 24-Epilolide-anti-bud pellet soluble solution on tobacco yield increasing effect
Conclusion: the test agent 30% 24-epibrassinolide-bud-inhibiting pellet soluble agent can effectively promote tobacco leaf growth, improve the single leaf weight of flue-cured tobacco, has good inhibition effect on tobacco axillary bud growth, can effectively reduce the quantity of axillary buds of tobacco plant with 2cm length, reduce the fresh weight of the axillary buds, has obvious application yield increasing effect, and has no adverse effect on main quality of tobacco leaf including Shi Muke value and nicotine content.
Application example five: field efficacy experiment of 30% 24-Epilopin lactone-bud inhibiting pellet solution (example 3) for promoting tobacco growth
1 purpose of experiment
The inhibition effect of the 30% 24-epibrassinolide-anti-bud pellet solution provided by the applicant on the growth of tobacco axillary buds, the application period, the use dosage and the safety on the growth of tobacco are clearly defined, and a basis is provided for registering the product.
2 materials and methods
2.1 experimental crops: tobacco, variety: gold 1025
2.2 Experimental agent treatment
TABLE 7 treatment with reagents
| Process numbering | Medicament | Dosage (dosage of preparation) | Dosage (effective component amount) |
| A | 30% 24-epibrassinolide-sprout inhibition pellet solution | 200 g/mu | 900g/hm 2 |
| B | 30% 24-epibrassinolide-sprout inhibition pellet solution | 250 g/mu | 1125g/hm 2 |
| C | 30% 24-epibrassinolide-sprout inhibition pellet solution | 300 g/mu | 1350g/hm 2 |
| D | 30% 24-epibrassinolide-sprout inhibition pellet solution | 500 g/mu | 2250g/hm 2 |
| E | 0.01% 24-epibrassinolide as a soluble solution | 33 g/mu | 0.05g/hm 2 |
| F | 30.2% bud-suppressing pill water aqua | 400 g/mu | 1812g/hm 2 |
| G | Manual bud picking control | / | / |
| H | Blank control | / | / |
3 date of experiment
Test start date: 2020, 6 months;
test completion date: 7 months in 2020.
4 application mode and investigation method same as application example III
5 experimental results
TABLE 8 influence of 30% 24-Epilolide-anti-bud pellet solution (example 3) on tobacco yield and quality
When the first central flowers of most tobacco plants in the tobacco field are open, topping is carried out when the top leaves are larger than 20cm, axillary buds larger than 2cm are beaten off, and 30% of 24-epibrassinolide-bud inhibition pellet solution is uniformly sprayed on the middle part and the front face of the tobacco plants, so that the growth of tobacco can be obviously regulated, the yield of tobacco is improved, and no adverse effect is caused on tobacco quality indexes such as nicotine content, potassium-chlorine ratio and Shi Muke value.
Application example six: field efficacy experiment of 30% 24-epibrassinolide-sprout inhibition pellet solution for promoting tobacco growth
1. Purpose of test
The 30% 24-epibrassinolide and anti-bud pellet soluble agent produced by Shaanxi Mebang pharmaceutical industry group Co., ltd is verified to have the effect of regulating tobacco and the safety, and technical data is provided for pesticide registration and production application.
2 materials and methods
2.1 test agent treatment
TABLE 9 treatment with reagents
2.2 test subjects and crops
Test crop: the variety is "cloud smoke 87".
2.3 time of application and method
The first flower of most tobacco plants in the tobacco field is opened (7 months and 2 days), the top is cut when the top leaf is more than 20cm, and the axillary buds more than 2cm are cut off, and the product is uniformly sprayed on the front surface of the middle part and the upper leaf surface of the tobacco plant, and then the medicine is applied once. The artificial bud is smeared once every 7 days, and 3 buds are smeared.
Spraying, wherein the water consumption per hectare is 450L.
2.4 investigation methods and times.
Harmful investigation: the tobacco was observed for phytotoxicity 3 days (2021.7.5), 5 days (2021.7.7) and 7 days (2021.7.9) after administration.
The number of axillary buds was investigated once 15 days (2021.7.17), 30 days (2021.8.1) and 40 days (2021.8.11) after administration, and the axillary buds were weighed fresh at the time of the last investigation (2021.8.11).
The maximum leaf length and maximum leaf width of the upper, middle and lower leaves of tobacco were measured 20 days after application (2021.7.22), respectively.
And (5) a tobacco harvesting period (2021.7.27-2021.8.20), measuring the yield of tobacco in batches, calculating the yield increase, selecting middle tobacco, and measuring the quality of indexes such as potassium content, chlorine content, nicotine content, reducing sugar content and the like in 8 months and 23-31 days.
During the test, the effect of the test agent on other non-target organisms was observed.
3 calculation method and investigation method are the same as in fourth embodiment
4 results and analysis
TABLE 10 results of 30% 24-Epilolide-anti-bud pellet soluble solution on tobacco yield and quality
According to the test result, under the test condition, the first central flowers of most tobacco plants in the tobacco field are opened, the top leaves are topped when being more than 20cm, axillary buds which are more than 2cm are removed, 30% 24-epibrassinolide/bud inhibition pellet solution with the effective component of 900-2250 g/hectare is uniformly sprayed on the front surface of the leaf surfaces in the middle part of the tobacco plants and above, and the tobacco leaves are treated for 1 time, so that the growth of the axillary buds of tobacco can be obviously inhibited, the improvement of the tobacco yield is promoted, and the quality of tobacco leaves is not affected.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the invention, and these should be considered to be within the scope of the invention.
Claims (3)
1. A plant growth regulating composition for regulating tobacco growth, characterized by: the effective active ingredients of the composition are 24-epibrassinolide and anti-sprouting agent, and one or more of wetting agent, salifying agent, solvent, PH regulator and defoamer are added to prepare a soluble agent, wherein the weight ratio of the 24-epibrassinolide to the anti-sprouting agent is 1:29999.
2. The composition of claim 1, wherein: the total weight of 24-epibrassinolide and the anti-sprouting pellet in the composition is 9% -51%.
3. The composition of claim 2, wherein: the total weight of 24-epibrassinolide and the anti-sprouting pellet in the composition is 30%.
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