CN116832119A

CN116832119A - Application of flavored honeycomb pills in preparation of medicines for treating lymphadenitis

Info

Publication number: CN116832119A
Application number: CN202310900502.9A
Authority: CN
Inventors: 解红霞
Original assignee: Affiliated Hospital Of Inner Mongolia Medical University Cardiovascular Research Institute Of Inner Mongolia Autonomous Region
Current assignee: Affiliated Hospital Of Inner Mongolia Medical University Cardiovascular Research Institute Of Inner Mongolia Autonomous Region
Priority date: 2023-07-20
Filing date: 2023-07-20
Publication date: 2023-10-03

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to application of Jiawei honeycomb pills in preparation of medicines for treating lymphadenitis. The traditional Chinese medicine composition (namely the preparation raw materials of the flavored honeycomb pills) provided by the invention is based on clinical curative effects and dialectical treatment of traditional Chinese medicine, and the weight optimal proportion of each traditional Chinese medicine is finally determined by combining clinical cases with animal pharmacodynamics experiments, in-vitro cell experiments and bacterial experiments.

Description

Application of flavored honeycomb pills in preparation of medicines for treating lymphadenitis

Technical Field

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to application of Jiawei honeycomb pills in preparation of medicines for treating lymphadenitis.

Background

Lymphadenitis is commonly seen in clinic and is divided into acute lymphadenitis and chronic lymphadenitis, a large number of statistics of morbidity are not available for reference at present, about 10% of people who visit departments are involved in diagnosis, and etiology and pathogenesis are still medical problems, so that students consider that the lymphadenitis is related to virus infection and autoimmunity.

Lymphadenitis is a self-limiting disease with good prognosis, but chronic lymphadenitis often recurs for a plurality of times and is not cured for a long time, and the chronic lymphadenitis is easily developed into tissue cell necrotizing lymphadenitis. The surgery mostly adopts antibiotics or operation treatment for acute lymphadenitis, and the antibiotics treatment is easy to generate drug resistance, so that the treatment effect is affected, and the operation treatment can cause damage to the body of a patient. In addition, most patients with chronic lymphadenitis do not seek medical treatment or do not know that the patients can take medicine, the patients wait for self-healing, and few patients repeatedly attack or progress to tissue cell necrotizing lymphadenitis, so that the treatment difficulty is increased.

The traditional Chinese medicine preparation has the advantages of small toxic and side effects, no drug resistance and capability of avoiding the damage caused by the operation to the body of a patient, is an ideal treatment method at present, but has no traditional Chinese medicine preparation with good treatment effect on both acute lymphadenitis and chronic lymphadenitis at present.

Disclosure of Invention

In order to solve the problems, the invention provides an application of flavored honeycomb pills in preparing medicines for treating lymphadenitis. The traditional Chinese medicine composition (namely the preparation raw material of the flavored honeycomb pills) has good treatment effect on acute lymphadenitis and chronic lymphadenitis, is safe and free of toxic and side effects, and can be used for preparing medicines for radically treating acute and chronic nonspecific lymphadenitis and specific lymphadenitis.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides application of a traditional Chinese medicine composition in preparing a medicament for treating lymphadenitis, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 10-30 parts of nidus vespae, 5-27 parts of subprostrate sophora, 5-27 parts of barbed skullcap herb, 5-27 parts of Indian iphigenia bulb, 5-15 parts of cornu cervi degelatinatum, 5-27 parts of Chinese pulsatilla root, 5-15 parts of radix sophorae flavescentis, 5-15 parts of liquorice, 5-15 parts of radix bupleuri, 5-12 parts of green tangerine peel and 0.378-2 parts of musk.

Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 30 parts of nidus vespae, 27 parts of subprostrate sophora, 27 parts of barbed skullcap herb, 27 parts of Indian iphigenia bulb, 15 parts of cornu cervi degelatinatum, 27 parts of Chinese pulsatilla root, 15 parts of radix sophorae flavescentis, 15 parts of liquorice, 15 parts of radix bupleuri, 12 parts of green tangerine peel and 0.378 part of musk.

Preferably, the lymphadenitis comprises one or more of acute nonspecific lymphadenitis, chronic nonspecific lymphadenitis, acute specific lymphadenitis and chronic specific lymphadenitis.

Preferably, the dosage form of the medicament comprises pills, capsules, oral liquid, tablets or powder.

Preferably, the preparation method of the pill comprises the following steps:

mixing nidus Vespae, radix Sophorae Tonkinensis, herba Scutellariae Barbatae, pseudobulbus Cremastrae seu pleiones, cornu Cervi Degelatinatum, radix Pulsatillae, radix Sophorae Flavescentis, glycyrrhrizae radix, bupleuri radix and pericarpium Citri Reticulatae viride, and pulverizing to obtain mixed medicinal powder;

Mixing and grinding musk and ethanol solution to obtain mixed solution;

mixing the mixed powder and the mixed solution, and refining honey into pills, thereby obtaining the pills, namely flavored honeycomb pills.

Preferably, the mixed powder is 100 mesh powder.

Preferably, the volume weight ratio of the ethanol solution to the musk is 5-10 mL:1g; the volume concentration of the ethanol solution was 75%.

Preferably, the temperature of the refined honey is less than or equal to 60 ℃.

Preferably, the crushing further comprises sterilizing the crushed medicinal powder; the sterilization method comprises the following steps: a constant temperature heat sterilization method or a pressure sterilization method;

the temperature of the constant-temperature heating sterilization method is 100 ℃, and the sterilization time is 1h;

the pressure of the pressure sterilization method is 0.8kg/cm ² The sterilization temperature is 115 ℃ and the sterilization time is 30min.

Preferably, the method for mixing the mixed powder and the mixed solution comprises an equal-amount mixing and grinding method.

The beneficial effects are that:

the invention provides application of a traditional Chinese medicine composition in preparing a medicament for treating lymphadenitis, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 10-30 parts of nidus vespae, 5-27 parts of subprostrate sophora, 5-27 parts of barbed skullcap herb, 5-27 parts of Indian iphigenia bulb, 5-15 parts of cornu cervi degelatinatum, 5-27 parts of Chinese pulsatilla root, 5-15 parts of radix sophorae flavescentis, 5-15 parts of liquorice, 5-15 parts of radix bupleuri, 5-12 parts of green tangerine peel and 0.378-2 parts of musk. The traditional Chinese medicine composition is based on clinical curative effect and dialectical treatment of traditional Chinese medicine, the weight optimal proportion of each traditional Chinese medicine is finally determined by combining clinical cases with animal pharmacodynamics experiments, in-vitro cell experiments and bacterial experiments, and the traditional Chinese medicine composition has special curative effect on acute and chronic nonspecific lymphadenitis and specific lymphadenitis, is clinically used for more than 20 years, and has stable quality standard, safety and effectiveness. The compatibility principle of the traditional Chinese medicine composition provided by the invention is as follows: the nidus vespae is slightly sweet and slightly salty in taste, has a flat nature, enters liver and stomach meridians, is a good medicine for eliminating toxin and treating sore, and dispersing swelling and relieving pain, and can treat some diseases of the neck, such as lymphadenitis, tuberculous lymphadenitis, sore throat and the like, by the stomach meridians going through the neck, and is a monarch drug; the bupleurum has strong fragrance, slightly ascending, dispersing and dredging, regulating liver qi and good dispersing and upper-jiao depression; qing Pi Weixin, its qi is strong, it settles down, breaks liver meridian qi stagnation, and is good for dredging depression of lower energizer; the bupleurum and the green tangerine peel are ministerial drugs, and are used together, the ascending and descending are suitable, the upward and downward movement is realized, the qi stagnation can be relieved, the qi stagnation can be realized, the qi stagnation can be dispersed, and the efficacy of the drug is more strong than that of a general liver soothing and qi regulating agent; the edible tulip, the subprostrate sophora root, the pulsatilla root, the barbed skullcap herb, the musk and the cornu cervi degelatinatum are taken as adjuvant drugs, wherein the edible tulip is sweet in nature, slightly spicy and cold, has little toxicity, and has the effects of clearing heat, removing toxicity, reducing swelling and resolving masses; the radix sophorae tonkinensis has the effects of clearing heat and detoxicating, detumescence and relieving sore throat, is mainly used for treating symptoms such as fire toxin accumulation, tonsillitis pharyngitis, sore throat, gum swelling and pain, sore mouth and tongue, and the like, and has the effect similar to that of the edible tulip; the pulsatilla root has the effects of clearing heat and detoxicating, resolving phlegm and resolving masses, and resolving carbuncles and resolving masses and is mainly used for treating sores and carbuncles; the sculellaria barbata is pungent and bitter, is cold, enters lung, liver and kidney channels, and has the effects of clearing away heat and toxic materials, removing blood stasis and stopping bleeding, and inducing diuresis to alleviate edema; musk is a good product for activating blood and dredging meridians and relieving pain, and can treat all symptoms of blood stasis regardless of time, and a small amount of musk is used for taking the effects of activating blood and dredging meridians; cornu Cervi Degelatinatum is a yang-tonifying medicine, and has effects of warming kidney, supporting yang, improving primordial qi, and salty taste, softening and resolving hard mass, warming and dredging collaterals, and relieving swelling; bitter licorice has bitter taste and cold property, has the effects of clearing heat and detoxicating, eliminating dampness and killing parasites, and is externally used for sore and furuncle and swelling and pain; licorice root, radix Glycyrrhizae can regulate the actions of the other drugs and is used together with Ku Licorice root as a guiding drug. The medicines are combined and have unique clinical effects, the chronic recurrent lymphadenitis can be radically cured after being used for more than 20 years, the medicine is safe and has no toxic or side effect, the total effective rate of clinical use can reach 75 percent, and the cure rate can reach 50 percent.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments will be briefly described below.

FIGS. 1 and 2 are T1WI results for the model set;

FIG. 3 is a T2WI result for a model set;

FIG. 4 is a T1WI result for a blank;

FIG. 5 is an ultrasound image of lymphadenectasis in a model group given two weeks of normal saline;

FIG. 6 is an ultrasonic view of the recovery of lymphadenectasis in a model group given the drug prepared in example 4 for two weeks;

FIG. 7 is an ultrasound image of lymphadenectasis in a model group given the drug prepared in example 5 for two weeks;

FIG. 8 is an ultrasound image of lymphadenectasis in a model group given the drug prepared in example 6 for two weeks;

FIG. 9 is a view showing the histopathology of lymph nodes in a model group taken with normal saline for two weeks;

FIG. 10 is a view showing the histopathology of lymph nodes of a model group taken for two weeks with the drug prepared in example 4;

FIG. 11 is an ultrasonic view of lymphadenectasis in a model group given the drug prepared in comparative example 1 for two weeks;

FIG. 12 is an ultrasonic view of lymphadenectasis in a model group given the drug prepared in comparative example 2 for two weeks;

FIG. 13 is an ultrasonic view of lymphadenectasis in a model group given the drug prepared in comparative example 3 for two weeks;

FIG. 14 is an ultrasonic view of lymphadenectasis in a model group given the drug prepared in comparative example 4 for two weeks;

Fig. 15 is an ultrasonic view of lymphadenectasis in a model group given the drug prepared in comparative example 5 for two weeks.

Detailed Description

The invention has no special requirements on the sources of the components of the traditional Chinese medicine composition unless specified, and the traditional Chinese medicine composition is commercially available products well known to those skilled in the art.

The traditional Chinese medicine composition comprises 10-30 parts by weight of nidus vespae, preferably 20-30 parts by weight, and more preferably 30 parts by weight. The nidus vespae is slightly sweet and slightly salty in taste, has flat nature, can return liver and stomach meridians, is a good medicine for eliminating toxin and treating sore, dispersing swelling and relieving pain, and can treat some diseases of the neck, such as lymphadenitis, tuberculous lymphadenitis, sore throat and the like when the stomach meridians pass through the neck.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-15 parts of radix bupleuri, preferably 10-15 parts, and more preferably 15 parts.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-12 parts of green tangerine peel, preferably 10-15 parts of green tangerine peel, and more preferably 12 parts of nidus vespae.

One of the common dialectical types of lymphadenitis is liver-stomach fire toxin, which is caused by liver qi stagnation, long-term fire transformation, phlegm formation, ascending of phlegm fire and accumulation on the neck, so that the recipe of the medicine for treating the lymphadenitis has the effects of dispersing blood stasis, resolving hard mass, removing dampness and ascending of clear qi, the bupleurum and the green tangerine peel both have the functions of soothing liver and regulating qi, the bupleurum is fragrant, slightly clear and ascending, dispersing and dredging liver qi, good and dredging the upper-jiao depression, the green Pi Weixin is warm, the qi is fierce, subsides downwards, breaks liver meridian qi stagnation, good and dredging the lower-jiao depression, the two are used together, ascending and descending are proper, the qi stagnation can be dredged upwards and downwards, the qi stagnation can be dispersed, the medicine efficacy is stronger than that of a general liver soothing agent, and the bupleurum and the green tangerine peel are all ministerial medicines.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-27 parts of Indian iphigenia bulb, preferably 18-27 parts, and more preferably 27 parts. The lymphadenitis has the symptoms of red swelling and pain in the acute attack stage, and can be treated by heat-clearing and detoxifying medicines. The Indian iphigenia bulb has sweet, slightly pungent and cold properties, small toxicity, and has the effects of clearing heat and detoxicating, and detumescence and resolving hard mass, and can be used for treating carbuncle and furuncle, and lymphadenitis as adjuvant drug.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-27 parts of subprostrate sophora, preferably 18-27 parts of subprostrate sophora, and more preferably 27 parts of subprostrate sophora. The radix sophorae tonkinensis has the effects of clearing heat and detoxicating, detumescence and relieving sore throat, is mainly used for treating symptoms such as fire toxin accumulation, tonsillitis, sore throat, swelling and pain of the throat, swelling and pain of gums, sore mouth and tongue and the like, has the effect similar to that of the edible tulip, and is an adjuvant drug.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-27 parts of sculellaria barbata, preferably 18-27 parts, and more preferably 27 parts. According to the traditional Chinese medicine, the adverse blood is water, and the pathogenic factors of water dampness and blood stasis are often influenced mutually, so that proper blood circulation is required in the treatment, and the sculellaria barbata is pungent, bitter and cold and enters lung, liver and kidney channels; the barbed skullcap herb has the functions of clearing away heat and toxic matter, eliminating blood stasis, stopping bleeding, inducing diuresis and eliminating swelling.

The traditional Chinese medicine composition comprises 5-15 parts of cornu Cervi Degelatinatum, preferably 10-15 parts, and more preferably 15 parts, based on the mass parts of nidus Vespae. Both water and blood stasis are different in shape and are both pathogenic yin and are caused by unsmooth yang qi, and the treatment is to pay attention to the primordial qi and the operation thereof. Cornu Cervi Degelatinatum is a yang-tonifying herb, has effects of warming kidney and tonifying yang, improving primordial qi, and has salty taste and warm nature, and is effective in softening hardness and resolving hard mass, warming and dredging collaterals and relieving swelling.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-27 parts of pulsatilla chinensis, preferably 18-27 parts of pulsatilla chinensis, and more preferably 27 parts of pulsatilla chinensis. For the condition of liver qi stagnation, fire resolving and phlegm refining, the Chinese pulsatilla root has the effects of clearing heat and detoxicating, resolving phlegm and resolving masses, and resolving carbuncles and resolving masses, and can be used for treating sore and carbuncles and swelling, and can be used as adjuvant in acute episode of lymphadenitis.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 0.378-2 parts of musk, preferably 0.378-1 part, and more preferably 0.378 part. Musk is a good herb for activating blood and dredging meridians and relieving pain, and is used as an adjuvant drug for treating blood stasis syndrome of all ages.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-15 parts of radix glycyrrhizae, preferably 10-15 parts, and more preferably 15 parts. Bitter licorice is bitter in flavor and cold in nature, has the effects of clearing heat and removing toxicity, drying dampness and killing parasites, and is externally used for furuncle and swelling and pain, and is a guiding drug.

Based on the mass parts of the nidus vespae, the traditional Chinese medicine composition comprises 5-15 parts of liquorice, preferably 10-15 parts, and more preferably 15 parts. Licorice root, radix Glycyrrhizae regulates the action of other drugs and acts as a guiding drug.

The acute stage of lymphadenitis is usually distinguished as wind-heat phlegm-toxin syndrome and heat-toxin exuberance syndrome, the chronic stage is usually distinguished as liver depression phlegm-fire syndrome and yang deficiency phlegm coagulation syndrome, the treatment principle of treating cervical lymphadenitis in the acute stage and the chronic stage takes the "method of elimination" as the first step, the invention takes nidus vespae as the principal drug, radix bupleuri and green tangerine peel as the ministerial drugs, indian iphigenia bulb, subprostrate sophora root, chinese pulsatilla root, barbed skullcap herb, musk and cornu cervi degelatinatum as the adjuvant drugs, liquorice and radix glycyrrhizae as the conductant drugs, the clinical effect of the combination of the drugs is unique, the use of the drugs is over 20 years, the chronic recurrent lymphadenitis can be radically treated at one time, the safety has no toxic and side effect, and the total effective rate of clinical use can reach 75 percent, and the cure rate can reach 50 percent.

In the present invention, the dosage form of the drug preferably includes a pill, a capsule, an oral liquid, a tablet or a powder. The dosage form of the traditional Chinese medicine composition is not limited to pills, and can be any dosage form in pharmacy, such as capsules, oral liquid, tablets, powder and the like according to the requirement, and common auxiliary materials can be added according to the requirement of the dosage form.

The preparation method of the pill (namely flavored honeycomb pill) preferably comprises the following steps:

Mixing and grinding musk and ethanol solution to obtain mixed solution;

The invention mixes and pulverizes nidus vespae, subprostrate sophora, barbed skullcap herb, indian iphigenia bulb, cornu cervi degelatinatum, chinese pulsatilla root, bitter licorice, liquorice, radix bupleuri and green tangerine orange peel to obtain mixed medicinal powder. In the present invention, the mixed powder is preferably 100 mesh powder.

In the present invention, the pulverizing preferably further comprises sterilizing the pulverized powder; the method of sterilization preferably comprises: a constant temperature heat sterilization method or a pressure sterilization method;

the temperature of the constant-temperature heating sterilization method is preferably 100 ℃, and the sterilization time is preferably 1h;

the pressure of the autoclave is preferably 0.8kg/cm ² The sterilization temperature is preferably 115 ℃, and the sterilization time is preferably 30min.

The invention mixes and grinds musk and ethanol solution to obtain mixed solution. In the invention, the volume weight ratio of the ethanol solution to the musk is preferably 5-10 mL:1g, more preferably 5 to 6mL:1g, more preferably 2mL:0.378g; the volume concentration of the ethanol solution is preferably 75%.

After the mixed medicinal powder and the mixed solution are obtained, the mixed medicinal powder and the mixed solution are mixed, and the honeyed pill is prepared, so that the flavored honeycomb pill is obtained.

In the present invention, the method of mixing the mixed powder and the mixed liquid preferably includes a method of mixing with equal amounts.

In the present invention, the temperature of the refined honey is preferably 60℃or less, more preferably 50 to 60℃and still more preferably 60 ℃. The method for refining the honey into pills is not particularly required, and the method is well known to those skilled in the art.

In the present invention, the weight of the pellets is preferably 6g per pellet. The present invention preferably also provides a method of taking a pill, preferably comprising: two pills are taken once a day, 2 times a day, each time in the morning and evening, and 30 days is 1 course of treatment. The pill provided by the invention is not limited in age and disease parts when being applied, and the pediatric cervical lymphadenitis is taken according to weight loss, and has special curative effects on acute and chronic nonspecific lymphadenitis and specific lymphadenitis, and has good curative effects.

The lymphadenitis disclosed by the invention comprises one or more of acute nonspecific lymphadenitis, chronic nonspecific lymphadenitis, acute specific lymphadenitis and chronic specific lymphadenitis.

The flavored honeycomb pills are used as an intra-hospital preparation in a three-hospital for more than 20 years, and the main indication is hyperplasia of mammary glands, but the treatment effect of lymphadenitis is found to be more effective and quicker in the clinical use process and the treatment is thorough. The lymphadenitis patients treated by the flavored honeyed pills have good curative effects on both acute and chronic nonspecific lymphadenitis and specific lymphadenitis, and especially cases with chronic recurrent attacks can be radically cured at one time, which is the uniqueness of the medicine.

For further explanation of the present invention, the application of flavored honeycomb pellets provided by the present invention in preparing medicines for treating lymphadenitis is described in detail below with reference to the accompanying drawings and examples, but they are not to be construed as limiting the scope of the present invention.

Example 1

A traditional Chinese medicine composition capable of treating lymphadenitis comprises the following components in parts by weight: 100 parts of nidus vespae, 50 parts of subprostrate sophora, 50 parts of barbed skullcap herb, 50 parts of Indian iphigenia bulb, 50 parts of cornu cervi degelatinatum, 50 parts of Chinese pulsatilla root, 50 parts of bitter licorice, 50 parts of liquorice, 50 parts of radix bupleuri, 50g of green tangerine peel and 5 parts of musk.

Example 2

A traditional Chinese medicine composition capable of treating lymphadenitis comprises the following components in parts by weight: 200 parts of nidus vespae, 180 parts of subprostrate sophora, 180 parts of barbed skullcap herb, 180 parts of Indian iphigenia bulb, 100 parts of cornu cervi degelatinatum, 180 parts of Chinese pulsatilla root, 100 parts of bitter licorice, 100 parts of radix bupleuri, 100 parts of green tangerine peel, 100 parts of licorice and 10 parts of musk.

Example 3

A traditional Chinese medicine composition capable of treating lymphadenitis comprises the following components in parts by weight: 300 parts of nidus vespae, 270 parts of subprostrate sophora, 270 parts of barbed skullcap herb, 270 parts of Indian iphigenia bulb, 150 parts of cornu cervi degelatinatum, 270 parts of Chinese pulsatilla root, 150 parts of bitter licorice, 150 parts of radix bupleuri, 120 parts of green tangerine orange peel, 150 parts of liquorice and 3.78 parts of musk.

Example 4

An flavored honeycomb pill for treating lymphadenitis is prepared by the following method: preparing materials according to the weight part of the embodiment 1, respectively weighing 100g of nidus vespae, 50g of subprostrate sophora, 50g of barbed skullcap herb, 50g of edible tulip, 50g of cornu cervi degelatinatum, 50g of Chinese pulsatilla root, 50g of radix sophorae flavescentis, 50g of liquorice, 50g of radix bupleuri and 50g of green tangerine peel, mixing, crushing into 100-mesh fine powder, bagging, sealing, sterilizing in an electrothermal constant-temperature oven at 100 ℃ for 1h by a constant-temperature heating sterilization method (the constant-temperature heating sterilization method is referred to Zhu Chengshan 'study of sterilization of big honeyed pills', chinese journal of traditional Chinese medicine, 6 month of 1989, 14 th edition); weighing 5g of musk, adding 2.5mL of medical alcohol with volume concentration of 75%, grinding uniformly, mixing uniformly with sterilized fine powder according to an equal amount grinding method, mixing with honey and lump, mixing at 50 ℃ to prepare large honeyed pills, and preparing 6g each pill.

Example 5

An flavored honeycomb pill for treating lymphadenitis is prepared by the following method: 200g of nidus vespae, 180g of subprostrate sophora, 180g of barbed skullcap herb, 180g of Indian iphigenia bulb, 100g of cornu cervi degelatinatum, 180g of Chinese pulsatilla root, 100g of bitter licorice, 100g of bupleurum, 100g of green tangerine orange peel and 100g of liquorice are respectively weighed according to the weight parts of the preparation materials described in the example 2, mixed and crushed into 100 meshes of fine powder, and the fine powder is sterilized under pressure of 0.8kg/cm by a pressurization method ² Sterilizing at 115deg.C for 30min (for pressure sterilization method, see Zhang Hong, "pressure sterilization method for Chinese patent medicine final product", chinese patent medicine research, 1980, stage 1); weighing 10g of musk, adding 10mL of medical alcohol with volume concentration of 75%, grinding uniformly, mixing uniformly with sterilized fine powder according to an equal amount grinding method, mixing with honey and lump, mixing at 60 ℃, and preparing into big honeyed pills with 6g each.

Example 6

An flavored honeycomb pill for treating lymphadenitis is prepared by the following method: preparing materials according to the weight part of example 3, respectively weighing 300g of nidus vespae, 270g of subprostrate sophora, 270g of barbed skullcap herb, 270g of edible tulip, 150g of cornu cervi degelatinatum, 270g of Chinese pulsatilla root, 150g of radix glycyrrhizae, 150g of radix bupleuri, 120g of green tangerine peel and 150g of liquorice, mixing, crushing into 100-mesh fine powder, bagging and sealing, and sterilizing in an electric constant-temperature oven at 100 ℃ for 1h by a constant-temperature heating sterilization method (the constant-temperature heating sterilization method is Zhu Chengshan "study of large honey bolus sterilization", chinese journal of traditional Chinese medicine, 6 months in 1989, 14 th volume and 6 th period); weighing 3.78g of musk, adding 2mL of medical alcohol with volume concentration of 75%, grinding uniformly, mixing uniformly with sterilized fine powder according to an equal amount grinding method, mixing with honey and lump, mixing at 60 ℃, and making into big honeyed pills with 6g each.

Comparative example 1

A nidus Vespae capsule is prepared by the following method: respectively weighing 100g of nidus vespae, 50g of subprostrate sophora, 50g of barbed skullcap herb, 50g of Indian iphigenia bulb, 50g of cornu cervi degelatinatum, 50g of Chinese pulsatilla root, 50g of bitter licorice, 50g of liquorice, 50g of radix bupleuri and 50g of green tangerine orange peel, mixing and crushing into 100-mesh fine powder. Weighing 5g of musk, adding 2.5mL of medical alcohol with volume concentration of 75%, grinding uniformly, mixing with sterilized fine powder according to an equivalent mixing grinding method, bagging and sealing, sterilizing in an electrothermal constant temperature oven at 100 ℃ for 1h by a constant temperature heating sterilization method (the constant temperature heating sterilization method refers to the four parts of Chinese pharmacopoeia of 2020 edition, general rule 1421 sterilization method), filling powder into hard capsules, and preparing capsules (the four parts of Chinese pharmacopoeia of 2020 edition, general rule 0103 capsules of preparation) with 0.3g each capsule.

Comparative example 2

A honeycomb granule is prepared by the following method: respectively weighing 100g of nidus vespae, 50g of subprostrate sophora, 50g of barbed skullcap herb, 50g of Indian iphigenia bulb, 50g of cornu cervi degelatinatum, 50g of Chinese pulsatilla root, 50g of liquorice, 50g of bupleurum, 50g of green tangerine peel, soaking in 70% ethanol which is 6 times of the mass of the medicinal materials as a solvent for 24 hours, slowly percolating, collecting percolate, concentrating, drying and crushing into fine powder, mixing 50g of cornu cervi degelatinatum with the fine powder, and crushing into 100-mesh fine powder. Weighing 5g of musk, adding 2.5mL of medical alcohol with volume concentration of 75%, grinding uniformly, mixing uniformly with sterilized fine powder according to an equivalent mixing grinding method, granulating with 750 g of sucrose, proper amount of dextrin and proper amount of ethanol, drying, bagging, sealing, sterilizing in an electrothermal constant temperature oven at 100 ℃ for 1h by a constant temperature heating sterilization method (the constant temperature heating sterilization method refers to 2020 edition of Chinese pharmacopoeia, four parts, general rule 1421 sterilization method), mixing uniformly, and preparing (the preparation method refers to 2020 edition of Chinese pharmacopoeia, four parts, general rule 0101 granules), and packaging 5g per bag.

Comparative example 3

Flavored honeycomb pellets similar to example 4 except that the raw materials are prepared without nidus Vespae.

Comparative example 4

Flavored honeycomb pellets similar to example 4 except that the preparation material does not contain green tangerine peel.

Comparative example 5

Flavored honeycomb pellets similar to example 4 except that the preparation material does not contain edible tulip.

Application example 1

Analysis of the curative effects of the modified nidus Vespae pills prepared in examples 4 to 6

The diagnosis standards of the lymphadenitis in the traditional Chinese medicine and the western medicine are respectively as follows:

1. diagnostic criteria for traditional Chinese medicine

Wind-heat evil toxin type: the enlargement of submaxillary and cervical lymph nodes, obvious tenderness, removable pushing, unchanged skin color, fever, headache, soreness of limbs, sore throat and the like, thin and yellow tongue coating and superficial and rapid pulse.

Internal accumulation of phlegm-fire: the lymph nodes are obviously enlarged, have hard texture and are pressed for pain; the tumor is fixed, the push is not moved, and the feeling of fluctuation of the push is accompanied by high fever, polydipsia, anorexia, constipation, red tongue, yellow and greasy coating and rapid pulse.

2. Western diagnostic criteria

Acute lymphadenitis: the red swelling of human body is caused by sudden bacterial infection, and is characterized in that the illness is urgent, most patients suffer from swelling pain, reduced appetite, debilitation, headache and the like, few patients also have uncomfortable reactions such as fever, nausea and vomiting and the like, and the lymph parts also have swelling conditions and pain during compression.

Chronic lymphadenitis: the chronic inflammation of adjacent organs can cause symptoms such as repeated pain, repeated swelling and the like of the lymph nodes, the disease period is longer, the symptoms are not obvious due to acute lymphadenitis, but the swollen parts of the lymph nodes are harder, the pain feeling is not obvious when the lymph nodes are pressed, and then the condition that the lymph nodes gradually become smaller and even fade exists.

Nonspecific lymphadenitis: usually the onset is urgent, but the symptoms are numerous and mild, with only mild lymphadenectasis and pain. The symptoms of the traditional Chinese medicine composition are heavy, local redness and fever can occur, and obvious tenderness exists. In severe cases, the lymph nodes can continuously grow and even fuse into clusters, and sometimes abscess can also be formed to have fluctuation sensation, and even cause ulcer and pus. Nonspecific lymphadenitis is regional lymphadenitis caused by common bacterial or viral infection.

Specific lymphadenitis: pain is not obvious for patients, but the lymph nodes are continuously enlarged, and symptoms of fever, hypodynamia and local slight pain are often caused, and even symptoms of lymph node crumbling and purulence are caused. Specific lymphadenitis is generally associated with fungal infection, tubercle bacillus infection, and the like.

Diagnostic typing:

the Chinese medicine is typed: (1) pathogenic wind-heat toxin type, (2) phlegm-fire internal accumulation type.

Western medicine typing is comprehensively judged and typed according to four diagnostic standards, such as: the acute lymphadenitis is met, and meanwhile, whether the acute lymphadenitis is acute specific or acute nonspecific is determined according to specific and nonspecific diagnostic standards, so that the diagnosis and the typing are finally determined, wherein the specific typing is as follows: (1) acute specific lymphadenitis, (2) acute nonspecific lymphadenitis, (3) chronic specific lymphadenitis, and (4) chronic nonspecific lymphadenitis.

Efficacy assessment criteria: (1) healing: the swelling and pain of the lymph nodes and the systemic symptoms disappear, and the blood is normal; (2) effective: the swelling and pain of the lymph nodes are improved, the general symptoms gradually disappear, and the blood is normal; (3) ineffective: exacerbating local and systemic symptoms, even abscess formation, and increased blood normal white cell count.

The administration method comprises the following steps:

two pills are taken once a day, 2 times a day, each time in the morning and evening, and 30 days is 1 course of treatment.

The total number of cases taken and the evaluation results of the curative effects of the flavored honeyed pills prepared in the different examples after 1-6 treatment courses are shown in table 1, wherein the effective rate=the effective rate/the total rate×100%; cure rate = number of cure cases/total number of cases x 100%; inefficiency = number of invalid cases/total number of cases x 100%; total effective rate= (effective number + recovery number)/total number x 100%.

TABLE 1 clinical effects of patients suffering from lymphadenitis after taking the Chinese medicinal composition of the present invention

Group of	Example 4	Example 5	Example 6
				Case number of pathogenic wind-heat toxin	54	58	36
Internal accumulation of phlegm-fire	76	74	38
				Number of cases of acute nonspecific lymphadenitis	29	33	14
Number of cases of chronic nonspecific lymphadenitis	41	35	20
				Acute specific lymphadenitis cases	38	26	19
Number of cases of chronic specific lymphadenitis	22	38	21
				Total number of cases	130	132	74
Effective rate (%)	6.15	8.33	10.81
				Cure rate (%)	73.08	71.21	77.03
Inefficiency (%)	20.77	20.46	12.16
				Total effective rate (%)	79.23	79.54	87.84
Administration course of treatment	1～6	1～6	1～6

Note that: the total cases in Table 1 are the sum of the cases of pathogenic wind-heat and internal accumulation of phlegm-fire, or the sum of the cases of acute nonspecific lymphadenitis, chronic nonspecific lymphadenitis, acute specific lymphadenitis and chronic specific lymphadenitis, and are the total cases of two different types of Chinese medicine and Western medicine.

As shown in Table 1, the flavored honeycomb pills prepared by the invention have good treatment effect on lymphadenitis.

Typical cases

The following typical cases of the flavored honeyed pills are taken by the following methods: two pills are taken once a day, 2 times a day, each time in the morning and evening, and 30 days is 1 course of treatment.

Case one: zeng Mou men, 28 years old, 5 months and 12 days old in 2009 receive and admit a hospital for fever accompanied by emaciation for more than 1 month. The patient has no obvious induced continuous fever before 1 month, and the cervical multiple lymph nodes are enlarged and fused, and the enlarged lymph nodes have soybean size and obvious tenderness. The body temperature of the patient rises after the afternoon every day, the body temperature is 40.3 ℃ at most, the debilitation is obvious, the appetite is reduced, and the weight is obviously reduced. Lymphoma and tuberculosis are excluded, and infections of other parts are generally excluded. Cervical lymph node biopsies, pathology cues: chronic nonspecific lymphadenitis. Belonging to the treatment range of the invention, the body temperature is recovered to be within the range of 36.4-37.2 ℃ after taking the flavored honeycomb pills prepared in the embodiment 4 of the invention for 2 treatment courses, and the volume of the swollen lymph nodes is reduced to about one fourth of the original volume. The patient continues to take 2 courses of treatment in the process of re-diagnosis in 9 months and 23 days in 2009, and the symptoms of lymphadenectasis of the patient completely disappear in half a year of follow-up, so that no obvious discomfort is caused.

Case two: wei Mou A, 26 years old, 11 days of early diagnosis in 12 months 2010, is received and admitted for a half month or more of cervical lymphadenectasis due to fever with debilitation. The patients get fever before half a month, the body temperature is up to 39.6 ℃, the cervical lymph nodes are enlarged like the egg size, and the pathology of the outer hospital considers tuberculosis, and anti-tuberculosis treatment is required to be given. The patient further performs lymph node biopsy from an affiliated hospital of the university of inner Mongolian medical science, and is diagnosed as chronic specific lymphadenitis. After taking the flavored honeyed pill prepared in the embodiment 4 of the invention for 2 treatment courses, the cervical lymphadenectasis is reduced to the size of soybean, the fever is reduced, and the body temperature is 37.0 ℃. After the medicine is continuously taken for 2 months, the swelling completely disappears, no fever symptoms appear, and no recurrence exists in 1 year of follow-up.

Case three: xi Mou women, 20 years old, early diagnosis 3 months 5 days 2011, cervical lymphadenectasis for more than half a year. The doctor can diagnose chronic lymphadenitis. The preparation is firstly used as an antituberculosis drug of western medicines, is not effective for 4 months, and is combined with a traditional Chinese medicine preparation for softening hardness, resolving hard mass and eliminating scrofula for 2 months. When the patients are examined in detail, the cervical lymph nodes are enlarged, the small patients are like beans, the large patients are 18 like apricot, and the patients are not red, are not painful, have white tongue coating and have deep and wiry pulse. Syndrome of comprehensive pulse, think: for pulse sinking, qi stagnation; wiry pulse, liver pulse; tuberculosis skin color is unchanged, and phlegm nuclei are also present. The flavored honeycomb pills prepared in example 5 of the present invention showed an improvement in cervical lymphadenectasis after 1 course of treatment. After 1 month of treatment, swelling was significantly reduced and pain was relieved. After 1 month of consolidation, swelling and pain disappeared.

Case four: xu Mou male, 52 years old, 2011, 3 month, 23 first visit. The patients have low fever for 10 days, the heat peak is about 38.8 ℃, slight cough, no phlegm, chills, night sweats, headache, pharyngalgia, slight waist soreness during fever, 1 x 2cm of enlarged lymph nodes can be reached to the right neck, the patients can be seen in local clinics, no examination is carried out, and oral cold medicines and cefuroxime effects are poor, so that the auxiliary hospitals of the university of Mongolian medicine are solved. Physical examination: the right neck touches several enlarged lymph nodes, tenderness. Ultrasonic performance: the lateral neck is swept through multiple swollen lymph nodes, the cortex is thickened, and the hilar structure exists. The lymph node biopsy is chronic specific lymphadenitis. The doctor can directly prescribe the flavored honeycomb pills prepared in the embodiment 5 of the invention for the doctor, the symptoms are obviously relieved after the pills are taken for 1 month according to the course of treatment, the check swelling is obviously reduced, the patients can lose symptoms after taking for 1 course of treatment, and no obvious discomfort exists. No recurrence is caused in the following half a year.

Case five: women, 36 years old, are admitted to the hospital in 2011, 3 and 6 days due to chilly and fever for half a month. Checking: the body temperature is 38.0 ℃, and the right submaxillary part, the bilateral neck part and the lymph nodes which are enlarged in size of soybean group can be contacted, so that the body is hard, tender, movable, and has no red surface skin and low skin temperature. The heart, lung and abdomen are not abnormal, and the joints of the limbs are not red and swollen. Kidney function, biochemistry and normal blood lipid. The chest-correcting lateral position sheet and the cervical and lumbar vertebra sheet are not abnormal, the chest CT shows the bilateral pleural effusion, the B ultrasonic liver, gall and pancreas are not abnormal, the spleen is about 36cm thick, the length is about 114cm, the size is increased, and the electrocardiogram is normal. Double neck color Doppler ultrasound: multiple enlarged lymph nodes are visible. The right cervical lymph node biopsy pathology examination on day 4 of hospitalization showed: lymphadenitis. Clinically, acute nonspecific lymphadenitis is diagnosed, and lymphadenitis symptoms are relieved after the flavored honeyed pill prepared in the embodiment 6 of the invention is taken for 1 course of treatment. The patient is not obviously uncomfortable after follow-up for half a year after continuing to take 1 course of treatment.

Case six: zhang Mou women, 11 years old, 2011, 5 months and 4 days of initial diagnosis. The patient found a week of left submandibular swelling. Checking the body: the maxillofacial region is basically symmetrical left and right, has unlimited mouth opening, has a mouth opening degree of 92.5px, can touch a mass of a middle tumor in the left submandibular region, has a diameter of about 100px, is round-like, and is light in pressure pain. The swelling was not reached in the mouth. Imaging performance: the surface of the left submandibular gland and subcutaneous elliptical low-density occupation are uneven in density, most of the density is low, the adjacent skin and subcutaneous fascia are thickened, the submandibular gland is slightly enlarged, the annular reinforcement is carried out after the reinforcement, the annular wall thickness is uneven, the inner wall is under-polished, and the adjacent submandibular multiple lymph nodes are enlarged and reinforced. No obvious damage to the adjacent bone is seen. Pathological results: acute specific lymphadenitis. After taking the flavored honeyed pill prepared in example 3 of the invention for 1 treatment course, the swelling disappears and the symptoms disappear.

Case seven: she Mou the first diagnosis of men, 44 years old, 6 months of 2011, and 12 days of date takes 'intermittent hematuria for 2 years, lower abdominal soreness, pain, debilitation, edema under knee, nocturia for 3 months' as a complaint, and looks into the body: the body temperature is 36.5 ℃, the subcutaneous and cervical sternoclavicular mastoid, namely the outer edge of the muscle and the supraclavicular fossa can be closed, and 6-7 bags with the diameter of about 1.5-2.0 cm are hard and medium in quality, good in activity, free from tenderness and renal function: normal; cervical lymph node biopsies: the diagnosis is chronic specific lymphadenitis. After the flavored honeycomb pills prepared in the embodiment 6 of the invention are taken for 3 months, the patients have no ache, normal urine volume, obviously reduced subjective cervical lymph nodes, no local compression symptoms and no obvious discomfort after 1 year follow-up treatment.

Case eight: zhang Mou female, 55 years old, 7 months 8 days 2011, complain about chronic lymphadenectasis 5 years, no fever, neck pain, obvious neck pain, and physical examination: the body temperature is 36.3 ℃, the pulse is 76 times/min, the blood pressure is 96/60mmHg, the retroauricular and cervical sternocleidomastoid, namely the outer edge of the muscle and the supraclavicular fossa can be covered by 5-6 bags with the diameter of about 1.5-2.0cm, the quality is harder, the activity is good, no tenderness exists, the heart and lung auscultation is abnormal, the abdomen is soft, and the rest is abnormal; the chronic specific lymphadenitis is has been confirmed, in view of the above experience, after taking the flavored honeyed pill prepared in the embodiment 6 of the invention for 1 treatment course, the neck pain is obviously improved, after taking the pill for half a month, the patient can check the body, the size of the neck lymph node can reach 1.2-1.8 cm, the size is obviously reduced, the continuous treatment is carried out for 2 months, and the neck lymph node inclusion block completely disappears. Follow-up was continued for 3 months without recurrence.

Case nine: a man, 26 years old, 8 months in 2011, 14 days old, affiliated hospitals in the university of medical science in inner mongolia. There was a history of skin infection, with bilateral mandibular lymphadenectasis and pain. Is improved by treatment. Recurrent mandibular lymphadenectasis with subsequent occasional episodes. Checking: palpating obvious swelling of bilateral mandibular lymph nodes of 5X 2cm ² Size, medium hardness, mild tenderness, confirmed as acute nonspecific lymphadenitis. The flavored honeyed pill prepared in example 6 of the invention has the advantages of obviously reduced swelling of the lower jaw lymph nodes, soft texture and reduced pain after 1 treatment course. To consolidate the curative effect, the medicine is continuously taken for 1 course of treatment, and no recurrence is found in 1 year of follow-up.

Case ten: li Mou, female, 12 years old,students, 8 months 2011, visit me departments. There was a history of chronic rhinitis and recurrent episodes of bilateral mandibular lymphadenectasis. Checking: palpating bilateral mandibular lymphadenectasis, even on the right side, is of unequal size, about 1X 1cm ² The size, the quality is harder, and the pain is pressed. No abnormal changes were seen in the mouth, cervical lymph node biopsies: the diagnosis is chronic nonspecific lymphadenitis. After 1 treatment course, the flavored honeycomb pills prepared in the embodiment 4 of the invention have the advantages of swelling and pain disappeared, normal recovery and no recurrence after follow-up for 1 year.

Case eleven: guo Mou women, 22 years old, 10 months and 28 days 2011. The cervical lymphadenectasis is 4 years from the suffering, after the suffering, the patients are not examined and treated systematically, and the suffering is suffering from swelling, pain and swelling of the lymphadenectasis caused by unsmooth emotion or tiredness, and intermittent low fever is often caused. The affiliated hospitals of the university of Mongolian medical science require traditional Chinese medicine treatment. The pain of the cervical lymph node is caused by the diagnosis, and the cervical lymph node is pumped to the shoulder, and the symptoms of tiredness, weakness, emaciation, inappetence, vexation, insomnia, low fever, and fever in the palms and soles are caused. Pale tongue with thin and white coating and deep and thready pulse. Checking: 2 enlarged lymph nodes of soybean can be reached in front of left cervical sternoclavicular papillary muscle; the right neck has about 1 enlarged lymph node of broad bean; the right hip antefossa can touch 1 jujube pit swelling lymph node, has hard texture and pain, can move, is not adhered to skin, and is diagnosed as chronic specific lymphadenitis. After taking the flavored honeyed pill prepared in example 6 of the invention for 2 months, the spirit is improved, subjective symptoms are all eliminated, the swollen lymph nodes are all disappeared, and no recurrence is caused in follow-up 1 year.

Twelve cases: yang Mou men, 36 years old, were diagnosed with left cervical mass 1 month to 1 month and 24 days of 2012. The tumor is the size of broad bean, gradually increases to the size of table tennis, and has pain in the submandibular area, paroxysmal pain in the lower left back teeth, occlusal pain, low fever and pain in the lower left back teeth. Ultrasonic inspection: acute nonspecific lymphadenitis in subleft jaw region. After taking the flavored honeyed pill prepared in the embodiment 6 of the invention for 1 treatment course, the complaint pain is obviously improved, and the tumor is reduced. After 1 course of treatment, the tumor disappeared and the pain symptom disappeared.

Case thirteen: wang Mou A male, 19 years old, 4 days of initial diagnosis 2/2012, has a swelling of the bilateral tonsils, a jujube-shaped tumor under the mandible, hard movement, clear boundaries and obvious tenderness. After three days the submaxillary swelling increased to the egg size. Erythromycin is used once, and the compound neonomine has no obvious curative effect on inflammation. Cervical lymph node biopsies were performed: the diagnosis is acute specific lymphadenitis. After taking the flavored honeyed pill prepared in example 6 of the invention for 1 treatment course, the swelling and pain completely disappear. B-ultrasonic prompt after half a month of continuous administration: cervical lymph nodes were not abnormal. Patients were instructed to keep mental state mild, exercise proper, diet low in fat, periodic examination, follow-up for half a year, and no recurrence.

Case fourteen: he Mou the men, 33 years old, received the department of respiratory medicine from the clinic on day 2021, 2 and 4 due to "febrile 1 week". The patients feel fever after catching cold 1 week, the highest body temperature reaches 39 ℃, and the patients continuously feel fever in the morning and at night, aversion to cold, a little cough, expectoration and cough white phlegm are accompanied with neck pain, general ache and hypodynamia. Once in 2012, the patient was treated in the hospital at 1 and 30 days, and no obvious abnormality was seen in CRP, blood routine and chest CT. For oral treatment of oseltamivir 75mg bid and lotus flower antipyretic capsules 4 tid, the symptom improvement is not obvious. For further treatment, 2 nd 4 th 2012, the department of outpatient service is admitted to the hospital by "fever check" in the department of medical science of inner Mongolia affiliated to the respiratory department of the hospital. Checking: body temperature is measured at the time of patient admission: 38.2 ℃, clear spirit, poor spirit, no congestion in pharynx, no tonsil swelling, no purulent secretion on the surface of tonsil, little soybean-like increase of lymph node in left neck, tenderness, soft texture, general mobility, color ultrasound of lymph node and related examination, considering acute nonspecific lymphadenitis, after taking the flavored honeycomb pill prepared in the embodiment 5 of the invention for 1 treatment course, the enlarged lymph node is contracted, fever disappears, after taking 1 treatment course again, swelling completely disappears, and no fever symptom appears.

Case fifteen: fan Mou, female, 14 years old, the main cause of "find neck tumor 8 days, fever 7 days" was received in the auxiliary hospital of the university of inner Mongolian medical science at 6 months 29 days 2012. The blood checking routine of the infant before hospital admission is not obvious abnormal, the "lymphadenitis" is suspected, the traditional Chinese medicine decoction and the Chinese patent medicine are treated, the fever is generated at night on the day, the body temperature is up to 38.2 ℃, the heat potential is gradually increased, the heat peak is generated for 4 hours for 1 time, the cervical lymph node is gradually enlarged, the tenderness is obvious, the skin itch is accompanied, and the skin rash and the joint swelling and pain are avoided. On the day of admission, review of cervical color ultrasound indicated "diffuse enlargement of bilateral submandibular glands, bilateral cervical and submandibular multiple lymphadenectasis". Considering the possibility of acute specific lymphadenitis, the method is communicated with family members of children, and lymph node pathological biopsies are performed on 7 months and 8 days after the family members agree to clearly diagnose. After taking the flavored honeyed pill prepared in the embodiment 6 of the invention for 1 treatment course, the symptoms of swelling and pain are improved, and fever disappears.

Case sixteen: liu Mou first diagnosis of men, 7 years, 7 months, 5 days 2012. The height is 125cm, the body weight is 31kg, and the patient is in an affiliated hospital of the university of medical science of inner Mongolia due to repeated heating for 1 year and aggravated for 2 days. The infant is heated by unknown reasons in 2011, the body temperature fluctuation is 38-39 ℃, the cephalosporin antibiotics (the specific dosage is not detailed) are taken, no obvious effect is seen, the infant is diagnosed as Kawasaki disease after transfer, the infant is treated by dexamethasone, the infant is discharged after the fever is relieved, the infant is heated again after about two half months, and the infant is taken as lansoprazole capsule, 15mg each time, 1 time a day, and the infant is discharged after the fever is relieved. Fever is again generated in 1 st 2012 and 4 th 2012, and discharge is relieved after medication. Fever occurs 2 days ago (7.3 days in 2012), and the lymph nodes are enlarged before fever and are accessible, so that the patient is treated by the traditional Chinese medicine and is in an affiliated hospital of the university of medical science of inner Mongolia. Chronic specific lymphadenitis is shown after lymph node biopsy, and fever is obviously improved after taking the flavored honeyed pill prepared in the embodiment 6 of the invention for 2 treatment courses. The patient can continue taking 2 courses of treatment during the re-diagnosis, and the fever symptoms of the children patients who follow the follow-up one year disappear, and no obvious discomfort is caused.

Case seventeen: store somewhere, male, 22 years old. The patient enters an affiliated hospital of university of medical science of inner Mongolia at 5.13 days of 2013 due to 'intermittent lymphadenectasis for 3 years and fever for 11 days'. Patients develop fever up to 40 ℃ 3 years ago, visit the children's blood ward in the first hospital affiliated to the university of Harbin medicine, complain about splenomegaly, neck, armpit and multiple lymphadenectasis in abdominal cavity (detailed in detail), detailed diagnosis is not detailed, vitamin C, calcium and methylprednisolone (detailed dosage in use) are given during hospitalization for about 2 weeks, the illness state is obviously improved, leucocytes are recovered to be normal, and the lymph nodes and spleens are discharged after being reduced to the normal range. Patients were not uncomfortable after discharge and were not reviewed. After about 2 days of 4 months in 2013, patients eat spicy food, the right side neck lymphadenectasis is found, the size of a quail egg is about, the tenderness and the activity are poor, the patients are treated in a Shenzhen market hospital, the patients are given with traditional Chinese medicine treatment (in detail) and the illness state is not improved after the patients are orally taken for more than 1 month, and the pathological return of the neck tumor is about 16 days in 5 months in a medical university affiliated hospital of the inner Mongolia of the tunnel for further treatment: the diagnosis of immunohistochemistry is considered to be clear and definite for chronic nonspecific lymphadenitis. After 2 treatment courses of the flavored honeyed pills prepared in the embodiment 6 of the invention are orally applied, the swelling and pain is relieved, and the lymph node biopsy is normal. And then consolidating and applying for 2 treatment courses, the swelling and pain disappears, and the disease is cured.

Eighteen cases: chen Mou, female, age 48, was admitted on 5 months and 4 days 2014 due to "fever with cervical tumor 1 month, exacerbation 5 days". The patient has no cause of fever before 1 month, the temperature is 38.5 ℃ at most, with aversion to cold, debilitation, pharyngalgia and muscular soreness, 1 pain is reached to the left neck painless tumor, the patient is diagnosed with 'infection' in local clinic, and 'aciclo Wei Jia moxillin potassium clavulanate' is administered for a dead spot and symptomatic treatment for 5 days, and the temperature is gradually recovered to be normal; fever occurs again before 5 days, the body temperature reaches 39.0 ℃, with debilitation, aversion to cold and night sweat, with dizziness and headache, with joint muscle soreness and tongue ulcer, simultaneously the left neck enlargement lymph node rapidly increases, and the right neck lymph node enlargement occurs, which is then treated in the affiliated hospital of the university of medical science of inner Mongolia. The patient has clear consciousness, multiple enlarged lymph nodes can be seen on the double necks, the shape is irregular, the surface is not broken, the maximum diameter is about 3cm multiplied by 3cm, the palpation quality is hard, the mobility is poor, the pain is pressed, the armpit and the groin can touch the multiple enlarged lymph nodes, the quality is hard, the mobility is poor, and the pain is pressed. Left cervical lymph node pathology results showed acute nonspecific lymphadenitis. The flavored honeyed pill prepared in example 5 of the present invention has significantly improved symptoms after 1 treatment period. After 2 months of treatment, the heat is lost and the swelling is reduced. After 1 month of the consolidation medicine, no obvious discomfort exists.

Case nineteen: li Mou women, 19 years old, have not been married. The patient is in the department of I.A. for cough, expectoration and fever for 10 days in 5-18 days of 2016. Patients develop low fever after catching cold before 10 days, fever is about 1 time a day, the body temperature is not more than 38.0 ℃, oseltamivir antiviral and cefdinir antibacterial treatment is carried out for 3 days, fever is still generated, the body temperature rises to 40.0 ℃, then patients are treated by a school hospital, antiviral (ribavirin) and tenor oral treatment are carried out, the body temperature of the patients is not more than 38.0 ℃ and is still repeated every day, and the patients are more than afternoon. Visit at 11 days of 5 months in affiliated hospitals of university of inner Mongolian medical science, examine body after admission: body temperature: 37.8 ℃, pulse: 114 times/min, breath: 20 times per minute, blood pressure: 110/70mmHg, blood oxygen: 100% of pharyngeal congestion, II-degree swelling of tonsils, little white exudate adhesion on the surface, no suppuration, soft neck, and accessible left neck to a plurality of swollen lymph nodes. Soft texture, high mobility and mild tenderness. After taking the flavored honeycomb pills prepared in the embodiment 6 of the invention for 1 course of treatment, the swelling and pain is slowed down, the body temperature is recovered to be normal, and the fever symptoms are not recurred and the swelling and pain is disappeared after continuing to take for 1 course of treatment. The treatment is further consolidated for 1 month, and no recurrence is caused in 1 year of follow-up.

Case twenty: lv Mou Male, 36 years old. The neck was found to have a round, 2cm diameter, free of tenderness, and movable bag in one upper respiratory tract infection by 3 months, and was taken at local hospitals. The enlargement of the lymph nodes due to upper respiratory tract infection was considered, and the upper respiratory tract infection healed after 5 days of anti-inflammatory and symptomatic treatment, but the cervical mass did not resolve. Before 2 months, the patient had a sense of oppression in the neck and had a poor breathing. For further treatment, the patient was admitted to the auxiliary hospital at the university of Mongolian medical science at 10.10.2017. Two lymph nodes with the size of soybean can be reached under the left jaw of a patient, and the patient has no tenderness and good activity. Chronic congestion of the pharynx, no swelling of bilateral tonsils, and visible diffuse in the proliferated lymphatic follicles in the back wall of the pharynx. The neck is symmetrical, the skin is free from red swelling and bursting, a bag block with the diameter of 2cm can be contacted below the glossoid on the median line in the front of the neck, the boundary is clear, the surface is smooth, the bag is provided with a feeling of being soft, the bag can move up and down along with swallowing, and the strip-shaped bulge is not contacted with the upper end of the bag. The lymph node biopsy is used for diagnosing chronic nonspecific lymphadenitis caused by chronic pharyngitis. The symptoms are in the treatment range of the invention, so the symptoms are obviously improved after the flavored honeycomb pills prepared in the embodiment 4 of the invention are taken for 2 treatment courses. The patient is continuously taken for 2 courses of treatment in the re-diagnosis process, and the follow-up half year patient has no obvious discomfort

Comparative application example

The unique curative effect of the flavored honeycomb pill for treating lymphadenitis provided by the invention is fully proved by clinical case collection and tracking, formula optimization and clinical curative effect verification of the embodiment, and combination of rat lymphadenitis model establishment, rat cervical lymphadenitis B ultrasonic comparison and rat lymphadenitis tissue section pathological experiment comparison. However, it should be noted that the types of acute lymphadenitis, chronic lymphadenitis, specific lymphadenitis and nonspecific lymphadenitis mentioned in the present invention belong to the western medicine category, and the two types of wind-heat evil type and phlegm fire internal knot type mentioned in table 1 in application example 1 are two most common types summarized according to the dialectical type of the medicines used for clinically treating lymphadenitis in traditional Chinese medicine and in the added honeycomb pill clinic, and are listed separately for the purposes of illustration and calculation efficiency. However, whether western medicine typing or traditional Chinese medicine typing is adopted, the final effect of the flavored honeycomb pill provided by the invention is judged according to apparent red swelling and pain disappearance, pain disappearance and lymphadenectasis reduction or disappearance, and is also a main observation index of animal pharmacodynamics.

The rat modeling method provided by the invention belongs to the acute nonspecific lymphadenitis type theoretically, but the effect observation is combined with the comprehensive judgment of traditional Chinese and western medicine, the nonspecific lymphadenitis is mainly caused by staphylococcus aureus or hemolytic streptococcus, the western medicine is subjected to anti-infection treatment, and the flavored honeycomb pill provided by the invention has the effects of resisting bacteria and inflammation and has good treatment effect; specific lymph nodes, which are caused by a number of specific bacteria, include tuberculosis, parasites, etc., appear as swollen lymph nodes and as localized red, swollen and hot pain. The symptoms of red swelling and pain accord with the surgical characteristics of traditional Chinese medicine, and the flavored honeycomb pill provided by the invention can achieve the effects of clearing away heat and toxic materials, resisting inflammation and relieving pain and has excellent treatment effect.

The following is a pharmacodynamic animal experiment method and a part of experiment diagram:

1. establishment of lymphadenitis animal model and verification of success or failure of modeling

First, materials and methods

1. Experimental animal

30 rats, male and female, were not limited, and randomly aliquoted into 2 groups. (1) model set: preparing 10% saline solution from fresh egg yolk, fully emulsifying in a homogenizer to form egg yolk emulsion (Egg Yolk Emulsion), and injecting 0.5ml egg yolk emulsion into one side of hind leg (muscle) and flank (subcutaneous); (2) blank group: no treatment is done.

2. MRI examination

1.5T superconducting magnetic resonance imager, using head coil, conventional SE sequence, scanning field 150mm×150mm. Axial and coronal scans are mainly, sometimes complementary sagittal scans, T1WI: TR 510ms,TE 14ms,T2WI: TR 630 ms, TE 70ms; layer thickness 3mm,2 acquisitions. (1) The MRI examination time of the model group is 7 days after the first injection of the yolk emulsion; (2) blank group for any selected time.

3. Pathological examination

After MRI plain scan was completed, animals were sacrificed, upper popliteal fossa and internal iliac lymph nodes were exposed, lymph nodes were removed and the size of upper popliteal fossa lymph nodes was measured, 10% neutral formalin-immersed, paraffin-embedded, HE stained and visualized under light.

4. Evaluation method

The size (minor diameter) of the popliteal lymph node on T1WI was measured and the average calculated and the results are shown in Table 2; the signal intensities of the popliteal lymph nodes and surrounding muscles on T1WI, T2WI were measured using the region of interest method, and the signal intensity of the muscle was used as a reference, and the lymph node signal intensity divided by the muscle signal intensity was used as a normalized signal intensity, and the results are shown in Table 3 and FIGS. 1 to 4.

5. Statistical method

The size and signal intensity differences of the 2 groups of popliteal lymph nodes were evaluated by using SPSS10.0 statistical software package and fully random design analysis of variance method, and the differences of the 2 groups of average numbers were subjected to multiple comparison by using SNK-q test.

(II) results

1. Animal performance after molding

Model group: the tolerance to the egg yolk emulsion is good, the phenomena of local reaction, whole body condition damage and the like are avoided, and the enlarged popliteal lymph node can be reached within 6-7 days;

blank group: no obvious change was observed.

2. MRI performance

Lymph node size:

(1) Model group: multiple groups of lymph nodes are enlarged and MRI can show popliteal fossa, intra-iliac, and inguinal superficial lymph nodes; the anterior group and the posterior group of the intra-iliac lymph nodes are all shown at the bifurcation of the abdominal aorta, and 3-4 lymph nodes are all shown in this area; (2) blank group: the popliteal lymph node is smaller, but is clearly shown on both T1WI and T2 WI. Only 3 mice showed clear internal iliac lymph nodes on T1WI and were not readily identifiable on T2 WI. From table 2 it can be seen that the model group is larger than the popliteal lymph node in the blank group.

Table 2 shows the size of each group popliteal fossa lymph node (unit: mm;)

group of	Blank group (n=6)	Model group
			T1WI	5.2±0.6	9.1±1.1
Pathology of the disease	5.4±0.5	9.3±1.2

Lymph node signal characteristics:

(1) Model group: the boundary is clear, the signal is uniform, on T1WI, the signal is lower than fat, close to muscle tissue (see fig. 1 and 2); at T2WI, the signal is significantly higher than the muscle tissue (see fig. 3); (2) blank group: the border is clear, the signal is uniform, at T1WI the signal is lower than fat but close to muscle tissue (see fig. 4); at T2WI, the signal is higher than the muscle tissue. As can be seen from Table 3, there was no significant difference in signal intensity between the popliteal lymph nodes of group 2 in both T1WI and T2 WI.

Table 3 normalized signal intensities for each group popliteal lymph node (unit: sl;)

imaging sequence	Blank group	Model group
			T1WI	1.32±0.04	1.32±0.08
T2WI	6.09±0.06	6.10±0.09

2. Model group rat pharmacodynamics verification

Method of administration

The dose was determined for each group of rats calculated as equivalent dose ratio of 0.018 to the conversion of human body surface area. The model group with successful modeling administers 2.520 g.kg of medium dose aqueous drug solution based on the weight of each kg of rat ^-1 ·d ^-1 At the same time set upControl group, wherein 0.9% by mass of physiological saline was administered to each kg of rats at 1.250 g.kg ^-1 ·d ^-1 The method comprises the steps of carrying out a first treatment on the surface of the The medicines are prepared in examples 1 to 6 and comparative examples 1 to 5; the medicine concentration of the medicine aqueous solution is 0.241g/ml; the administration was performed by gavage 1 time a day for 14 days. Lymph nodes of rats administered with different drugs and physiological saline were measured using an ultrasonic diagnostic apparatus LOGIQE9, an ultrasonic probe L8-18i-D, and pathological sections were made of lymph node tissues of rats administered with the drugs prepared in example 4 and rats administered with physiological saline, and the results are shown in FIGS. 5 to 15.

As can be seen from fig. 5 to 15, the lymph node size of the rats given with physiological saline for two weeks was 40×78mm (fig. 5), the lymph node size of the rats given with the drug prepared in example 4 for two weeks was 0×0mm (fig. 6), the lymph node size of the rats given with the drug prepared in example 5 for two weeks was 20×15mm (fig. 7), the lymph node size of the rats given with the drug prepared in example 6 for two weeks was 15×10mm (fig. 8), the number of lymph node tissue of the rats given with physiological saline for two weeks was increased, the number of glandular cells in the small leaf was increased, the number of the epithelial cells and secretions seen in the lumen was increased, the catheter was expanded, the swelling was apparent (fig. 9), the number of the small leaf cells of the rat lymph node tissue for two weeks given with the drug prepared in example 4 was reduced, the secretions were mainly catheter, the symptoms of the lymph node in the small leaf were significantly reduced, the symptoms of the swelling were significantly improved (fig. 10), the lymph node size of the rats given with the drug prepared in comparative example 1 for two weeks was 30×10mm (fig. 11), the lymph node tissue of the rats given with the two weeks was 20×10mm (fig. 2), the lymph node tissue of the rats given with the two weeks was prepared in the drug prepared in comparative example 1, the two weeks was 20×14 mm, and the lymph node tumor was significantly lost (see the lymph node tumor was prepared in the invention) and the two-35 mm (see the invention) and the lymph node tumor was significantly treated mice were prepared in the invention).

Wherein, the formula and the proportion of the comparative example 1 are the same as those of the example 4, and only the dosage form is changed into capsules; the composition and the proportion of comparative example 2 are exactly the same as those of example 4, but the extraction process is changed, and the dosage form is granule; the formula of comparative example 3 lacks nidus Vespae, other medicinal materials and the proportion are the same as those of example 4, and the dosage form is big honeyed pill; the formulation of comparative example 4 lacks radix Sophorae Tonkinensis, but retains nidus Vespae and Pseudobulbus Cremastrae seu pleiones, and other materials and proportions are the same as those of example 4, and the dosage form is big honeyed pill; the formulation of comparative example 5 lacks edible tulip, and other medicinal materials and proportions are completely the same as those of example 4, and the dosage form is big honeyed pill; as can be seen from fig. 6, 11 and 12, the improved dosage forms and the post-process treatment effects of comparative examples 1 and 2 were poor, and the drug of comparative example 2 was extracted and changed to the granule to have the worst treatment effect. The pill of example 4 is 6g each pill, the dosage is two pills once, the capsule dosage is only 0.3g according to the pharmacopoeia standard, the capsule and pill of the same medicine can be taken by the patient, the capsule can reach the effective rate of the pill only by taking 40 capsules each time, and a large amount of capsules are not used conventionally, so the medicine effect of the pill is obviously better than that of the capsule according to the conventional dosage.

As can be seen from fig. 13 and 15, comparative example 3 lacking nidus Vespae and comparative example 5 lacking edible tulip have significantly deteriorated therapeutic effects. In contrast, the medicines prepared in comparative example 4 were improved in the treatment effect by combining nidus Vespae and Pseudobulbus Cremastrae seu pleiones as the medicine pair (fig. 14), but the treatment effect was still inferior to that of the medicines prepared in example 4.

In conclusion, the traditional Chinese medicine composition (namely, the traditional Chinese medicine composition) provided by the invention has a good treatment effect on lymphadenitis, is safe and free from toxic and side effects, and can radically treat chronic recurrent cases at one time.

Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.

Claims

1. The application of the traditional Chinese medicine composition in preparing the medicine for treating lymphadenitis comprises the following components in parts by weight: 10-30 parts of nidus vespae, 5-27 parts of subprostrate sophora, 5-27 parts of barbed skullcap herb, 5-27 parts of Indian iphigenia bulb, 5-15 parts of cornu cervi degelatinatum, 5-27 parts of Chinese pulsatilla root, 5-15 parts of radix sophorae flavescentis, 5-15 parts of liquorice, 5-15 parts of radix bupleuri, 5-12 parts of green tangerine peel and 0.378-2 parts of musk.

2. The use according to claim 1, wherein the Chinese medicinal composition comprises the following components in parts by weight: 30 parts of nidus vespae, 27 parts of subprostrate sophora, 27 parts of barbed skullcap herb, 27 parts of Indian iphigenia bulb, 15 parts of cornu cervi degelatinatum, 27 parts of Chinese pulsatilla root, 15 parts of radix sophorae flavescentis, 15 parts of liquorice, 15 parts of radix bupleuri, 12 parts of green tangerine peel and 0.378 part of musk.

3. The use according to claim 1 or 2, wherein the lymphadenitis comprises one or more of acute non-specific lymphadenitis, chronic non-specific lymphadenitis, acute specific lymphadenitis and chronic specific lymphadenitis.

4. The use according to claim 1 or 2, wherein the dosage form of the medicament comprises a pill, capsule, oral liquid, tablet or powder.

5. The use according to claim 4, wherein the preparation method of the pill comprises the steps of:

mixing and grinding musk and ethanol solution to obtain mixed solution;

6. The use according to claim 5, wherein the mixed powder is a 100 mesh powder.

7. The use according to claim 5, wherein the volume/weight ratio of the ethanol solution to the musk is 5-10 mL:1g; the volume concentration of the ethanol solution was 75%.

8. The use according to claim 5, wherein the temperature of the refined honey is less than or equal to 60 ℃.

9. The use according to claim 5, wherein the pulverizing further comprises sterilizing the pulverized powder; the sterilization method comprises the following steps: a constant temperature heat sterilization method or a pressure sterilization method;

10. The use according to claim 5, wherein the method of mixing the powder and the liquid mixture comprises an equivalent method of grinding.