CN116754528A - Cell dynamic circumferential stretching device for real-time fluorescence imaging - Google Patents
Cell dynamic circumferential stretching device for real-time fluorescence imaging Download PDFInfo
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- CN116754528A CN116754528A CN202310555350.3A CN202310555350A CN116754528A CN 116754528 A CN116754528 A CN 116754528A CN 202310555350 A CN202310555350 A CN 202310555350A CN 116754528 A CN116754528 A CN 116754528A
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- 238000000799 fluorescence microscopy Methods 0.000 title claims abstract description 19
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- 230000000712 assembly Effects 0.000 claims abstract description 12
- 230000007246 mechanism Effects 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 238000003860 storage Methods 0.000 claims description 31
- 238000003825 pressing Methods 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 6
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- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 3
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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Abstract
The application discloses a cell dynamic circumferential stretching device for real-time fluorescence imaging, which is used for matching with a confocal microscope, and comprises a shell, wherein the top surface of the shell is open, the size of the shell is matched with the size of a placement area of the confocal microscope, and a sample and a stretching mechanism are arranged in the shell; the sample is integrally cast and formed, a placing part is arranged on the sample, the placing part is matched with the eyepiece of the confocal microscope in size, and a sealing part is detachably connected to the placing part; the stretching mechanism comprises a compression ring and a driving part, the size of the inner ring of the compression ring is matched with the size of an eyepiece of the confocal microscope, a plurality of connecting rod assemblies are arranged on the circumference of the side wall of the compression ring, the bottom ends of the connecting rod assemblies are spliced with the sample, an opening is formed in the inner bottom surface of the shell, the circle center of the sample corresponds to the circle center of the opening, and the size of the opening is larger than the size of an objective lens of the confocal microscope.
Description
Technical Field
The application belongs to the technical field of biomedical engineering, and particularly relates to a cell dynamic circumferential stretching device for real-time fluorescence imaging.
Background
Cells are in a complex three-dimensional microenvironment and are subject to interactions of various factors such as biochemical signals and mechanical stimuli. The tensile stimulus is used as a mechanical factor for simulating the stretch injury of a human body, and is widely applied to the research of cell mechanics, in particular to the drug therapy of the targeted osteoarthritis in the interrelation with a force sensitive ion channel. Currently, cell dynamic stimulation devices generally focus on applying unidirectional stretching to cells to simulate the effects of mechanical stretching stimulation on cell proliferation, differentiation, and phenotypes. In addition, all cell dynamic stimulation devices do not have the characteristics of being matched with a microscope. Thus, researchers cannot observe and record in real time the cell biological behavior during analysis of dynamic mechanical stimuli. If cells are subjected to dynamic mechanical stimulation and then transferred to a microscope for static observation and analysis, the difference of cell results caused by the time delay effect is a common concern of scientists at present.
The time delay effect is particularly embodied in two aspects, one is that when living cell immunofluorescence staining is carried out and changes of cell morphology, protein and calcium signals under mechanical stretching stimulation are observed, the current technology can only put the stained cells into a device to stretch to a specific deformation and then move the stained cells under a microscope for observation. This observation of cell morphology and its internal structure is still a static stimulation effect in nature, not a real-time dynamic cell behavior; secondly, when the dynamic space-time quantitative analysis of calcium signals, ions and the like of cells is carried out, the current technology can only carry out specific ion staining after unloading living cells, and then move to be observed under a microscope. In this process, the dynamic mechanical stimulation effect of living cells is lost, and real-time quantitative space-time analysis cannot be performed, so that a device for uniform dynamic stimulation of cells, which can be used with a confocal microscope and is used for real-time fluorescence imaging, is needed.
Disclosure of Invention
In order to solve the above technical problems, the present application provides a cell dynamic circumferential stretching device for real-time fluorescence imaging, which aims to solve or improve at least one of the above technical problems.
In order to achieve the above purpose, the application provides a cell dynamic circumferential stretching device for real-time fluorescence imaging, which is used for matching with a confocal microscope, and comprises a shell, wherein the top surface of the shell is open, the size of the shell is matched with the size of a placement area of the confocal microscope, and a sample and a stretching mechanism are arranged in the shell;
the sample is integrally cast and formed, a placing part is arranged on the sample and is matched with the eyepiece of the confocal microscope in size, and a sealing part is detachably connected to the placing part;
the stretching mechanism comprises a pressing ring and a driving part, the driving part is in transmission connection with the pressing ring, the size of the inner ring of the pressing ring is matched with the size of an eyepiece of the confocal microscope, a plurality of connecting rod assemblies are circumferentially arranged on the side wall of the pressing ring, and the bottom ends of the connecting rod assemblies are spliced with the sample;
the inner bottom surface of the shell is provided with an opening, the circle center of the sample corresponds to the circle center of the opening, and the size of the opening is larger than that of an objective lens of the confocal microscope.
Preferably, the length of the shell is 240mm, the width is 120mm, and the height is 55.49mm.
Preferably, the placing part comprises four cell storage tanks with the same size, and the four cell storage tanks are symmetrically arranged on the top surface of the sample in pairs.
Preferably, the diameter of the cell storage groove 4 is 18mm, the distance between the circle center diameters of any two adjacent cell storage grooves 4 is 15-22mm, and the diameter of the circle tangent line of the whole of the four cell storage grooves is 33-40mm.
Preferably, the distance between the bottom surface of the cell storage tank and the bottom surface of the sample is 1mm.
Preferably, the sealing part comprises an annular clamping groove, the annular clamping groove is formed in the top surface of the sample, the annular clamping groove is used for coating the four cell storage grooves, a transparent culture dish cover is detachably connected to the annular clamping groove, and the culture dish cover is made of PDMS materials in a ratio of 1:1.5-1:10.
Preferably, the diameter of the inner ring of the compression ring is 40-55mm.
Preferably, the connecting rod assembly comprises an upper connecting rod, the top end of the upper connecting rod is hinged with the side wall of the pressure ring, the bottom end of the upper connecting rod is hinged with a lower connecting rod, and the bottom end of the lower connecting rod is spliced with the sample.
Preferably, the drive part comprises two servo motors, the two servo motors are respectively positioned at two sides of the sample, the servo motors are fixedly arranged at the inner bottom surface of the shell, the output shaft of each servo motor is fixedly connected with a lead screw, the lead screw is connected with a push plate in a transmission manner, two connecting rods are fixedly connected between the push plates, two connecting rings are symmetrically fixedly connected at the top surface of the compression ring, and the two connecting rods are respectively arranged in the two connecting rings in a penetrating manner.
Preferably, an external speed regulation display is fixedly connected to one side of the shell, a driving plate and a control plate are fixedly installed in the external speed regulation display, the driving plate is electrically connected with the control plate, the control plate is electrically connected with the external speed regulation display, and the driving plate is electrically connected with two servo motors.
Compared with the prior art, the application has the following advantages and technical effects:
through planting required tensile cell in placing the portion to cover the portion of placing through sealing portion, can greatly reduce the cell pollution in the experimental process, and afterwards through placing the shell in the supporting region of placing of confocal microscope, and move down through drive portion drive clamping ring, can drive a plurality of link assemblies through the clamping ring and carry out even stretching to the sample, play the effect to the even stimulus of cell, and, because shell, portion of placing, clamping ring and open-ended size are according to the design of confocal microscope, can all realize the effect of real-time observation under confocal microscope 5 times mirror, 10 times mirror, 20 times mirror, 40 times mirror, 63 times mirror.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:
FIG. 1 is a schematic diagram of the overall structure of the present application;
FIG. 2 is a schematic view of the structure of the present application without the housing and the external speed adjusting display;
FIG. 3 is a schematic diagram of the structure of a sample according to the present application;
fig. 4 is a top view of the housing of the present application.
In the figure: 1. a housing; 2. a sample; 3. a compression ring; 4. a cell storage tank; 5. an annular clamping groove; 6. a culture dish cover; 7. an upper connecting rod; 8. a lower connecting rod; 9. a servo motor; 10. a screw rod; 11. a push plate; 12. a connecting rod; 13. a connecting ring; 14. externally connecting a speed regulation display; 15. a driving plate; 16. a control board; 17. an opening.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
In order that the above-recited objects, features and advantages of the present application will become more readily apparent, a more particular description of the application will be rendered by reference to the appended drawings and appended detailed description.
Referring to fig. 1-4, the embodiment provides a cell dynamic circumferential stretching device for real-time fluorescence imaging, which is used for matching with a confocal microscope, and comprises a shell 1, wherein the top surface of the shell 1 is open, the size of the shell 1 is matched with the size of a placement area of the confocal microscope, and a sample 2 and a stretching mechanism are arranged in the shell 1;
the sample 2 is integrally cast and formed, a placing part is arranged on the sample 2, the placing part is matched with the eyepiece of the confocal microscope in size, and a sealing part is detachably connected to the placing part;
the stretching mechanism comprises a compression ring 3 and a driving part, the driving part is in transmission connection with the compression ring 3, the size of the inner ring of the compression ring 3 is matched with the size of an eyepiece of the confocal microscope, a plurality of connecting rod assemblies are circumferentially arranged on the side wall of the compression ring 3, and the bottom ends of the connecting rod assemblies are spliced with the sample 2;
an opening 17 is formed in the inner bottom surface of the shell 1, the circle center of the sample 2 corresponds to the circle center of the opening 17, and the size of the opening 17 is larger than that of an objective lens of the confocal microscope.
Through planting required tensile cell in placing the portion to cover the portion of placing through sealing portion, can greatly reduce the cell pollution in the experimental process, then through placing shell 1 in the supporting region of placing of confocal microscope, and move down through drive division drive clamping ring 3, can drive a plurality of link assemblies through clamping ring 3 and evenly stretch sample 2, play the effect to the even stimulus of cell, and, because shell 1, portion of placing, clamping ring 3 and open-ended size are according to the design of confocal microscope, can be under the effect of real-time observation of confocal microscope.
Further optimizing scheme, shell 1 length is 240mm, and the width is 120mm, and the height is 55.49mm.
The confocal microscope matched with the confocal microscope is characterized in that the confocal microscope matched with the confocal microscope is a Leica confocal microscope, the placement area is trapezoid, the specific size is 264mm long and 152mm wide, a black rectangular visual field area is arranged in the trapezoid placement area, the specific size is 155mm long and 109mm wide, the rectangular visual field area is provided with a clamp, the specific size is 112.5mm maximum length, 34.5mm minimum length and 67.5mm width, and the diameters of an objective lens and an eyepiece are 33mm and 40mm respectively. Further, the movable section in the entire Z-axis direction is less than 63mm.
The diameter of the bottom opening 17 in the shell 1 is 60mm and is larger than the size of the objective lens, so that the objective lens can observe conveniently.
The highest position of the pressure ring 3 does not exceed the top surface of the housing 1.
According to a further optimization scheme, the placement part comprises four cell storage tanks 4 with the same size, the four cell storage tanks 4 are symmetrically arranged on the top surface of the sample 2 in pairs, the diameter of each cell storage tank 4 is 18mm, the distance between circle center diameters of any two adjacent cell storage tanks 4 is 15-22mm, and the diameter of a circle tangent line of the whole of each cell storage tank 4 is 33-40mm.
The four cell storage tanks 4 are consistent in size, symmetrical in position and even in stress during experiments, and can be used as a control group mutually by adding different factors, so that the effect of simultaneous stimulation of the control group and the experimental group is realized, and the experimental efficiency is improved.
In a further preferred embodiment, the distance between the bottom surface of the cell storage tank 4 and the bottom surface of the sample 2 is 1mm.
Thus, the confocal microscope objective lens can be conveniently and clearly observed.
Further optimizing scheme, sealing portion includes annular draw-in groove 5, and annular draw-in groove 5 is offered at sample 2 top surface, and annular draw-in groove 5 cladding four cell storage grooves 4, and annular draw-in groove 5 is last to be detachably connected with transparent culture dish lid 6, and culture dish lid 6 is made by 1:1.5-1:10's PDMS material.
The thickness of the annular clamping groove 5 is 5mm, the depth is 3mm, the structure of the culture dish cover 6 is matched with the structure of the culture dish cover, the culture dish cover 6 can be synchronously expanded along with the stretching of the sample 2 by inserting the culture dish cover 6 on the annular clamping groove 5, and cells in the four cell storage grooves 4 can be protected, so that the cell pollution in the experimental process is reduced.
In a further optimized scheme, the diameter of the inner ring of the compression ring 3 is 40-55mm.
Avoiding interference with confocal microscope eyepiece observation.
Further optimizing scheme, the link assembly includes upper connecting rod 7, and upper connecting rod 7 top is articulated with clamping ring 3 lateral wall, and upper connecting rod 7 bottom articulates there is lower connecting rod 8, and lower connecting rod 8 bottom is pegged graft with sample 2.
Through the perpendicular downward movement of clamping ring 3, can drive upper connecting rod 7 and produce the angular offset to promote the outside removal of lower connecting rod 8 by upper connecting rod 7, thereby by the synchronous expansion of a plurality of lower connecting rods 8, realize the tensile to sample 2, culture dish lid 6 expands because of deformation simultaneously, thereby plays the effect to the even stimulation of cell in four cell storage tank 4 in sample 2.
Further, the connecting rod assemblies are preferably 8 groups, and the 8 groups of connecting rod assemblies are arranged at equal intervals.
When the sample 2 is poured and molded, a plurality of jacks are formed in the circumferential direction of the outer ring of the sample 2, so that the bottom ends of a plurality of lower connecting rods 8 are conveniently inserted.
Further optimizing scheme, drive division includes two servo motor 9, and two servo motor 9 are located sample 2 both sides respectively, and servo motor 9 fixed mounting is in the interior bottom surface of shell 1, and servo motor 9's output shaft rigid coupling has lead screw 10, and the transmission is connected with push pedal 11 on the lead screw 10, and the rigid coupling has two connecting rods 12 between two push pedal 11, and the symmetry rigid coupling of clamping ring 3 top surface has two go-between 13, and two connecting rods 12 wear to establish respectively in two go-between 13.
The two servo motors 9 respectively drive the two lead screws 10 to synchronously rotate, so that the two push plates 11 can synchronously displace, and the pressure ring 3 is driven to vertically move downwards by the two connecting rods 12 penetrating through the connecting rings 13.
The highest position of the push plates 11 does not exceed the top surface of the housing 1.
According to a further optimization scheme, one side of the shell 1 is fixedly connected with an external speed regulation display 14, a driving plate 15 and a control plate 16 are fixedly installed in the external speed regulation display 14, the driving plate 15 is electrically connected with the control plate 16, the control plate 16 is electrically connected with the external speed regulation display 14, and the driving plate 15 is electrically connected with the two servo motors 9.
The driving plate 15 can be controlled by the control plate 16 to control the frequency of the servo motors 9, the two servo motors 9 are electrically connected with the driving plate 15 in a parallel connection mode, the synchronous control effect of the two servo motors 9 can be achieved, and the external speed regulation display 14 can display and debug numerical values.
Furthermore, the surface of the cell storage groove 4 can be provided with a photoetching silicon plate, and when the photoetching silicon plate is put into the four cell storage grooves 4, the surface of the formed cell storage groove 4 can form small holes for storing single cells, namely the application can also be used for dynamically regulating and controlling the volume of the single cells.
The implementation steps are as follows:
firstly, pouring the materials into two casting molds (matched with the structures of the sample 2 and the culture dish cover 6) respectively, wherein the pouring ratio is 1:20 and 1: and 5. After molding at high temperature, demolding to form a sample 2 and a culture dish cover 6. The sample 2 and the culture dish cover 6 were subjected to ultraviolet sterilization, a cell suspension of a certain concentration was dropped into the cell storage tank 4, the culture dish cover 6 was covered in the card tank, and the culture dish was put into the incubator for culturing for one day, and during this period, the incubator including the housing 1 and the stretching mechanism was subjected to high-temperature sterilization.
After one day, after the cells are attached, immunofluorescence staining is carried out on the cells, the cell nucleus and the cell skeleton are specifically stained, and after the staining is finished, the sample and the stretching mechanism are fixed and placed in a rectangular visual field area of a confocal microscope.
Then, the confocal microscope was opened, the objective lens was focused on the cell storage tank 4 in the sample 2, and an appropriate magnification lens was selected.
Finally, the external speed regulation display 14 is opened, the rotating speed of the servo motor 9 is set, the sample 2 is uniformly stretched, the dynamic uniform stimulation of cells in the cell storage tank 4 is realized, and at the moment, the change of the cell morphology along with the stretching after dyeing can be seen in real time under a confocal microscope.
The application can observe the cell morphology by real-time fluorescence imaging of the uniform dynamic mechanical stimulation cells, and can also realize real-time quantification of the calcium signals of the dynamic uniform stimulation of living cells.
In the description of the present application, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present application, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present application.
The above embodiments are only illustrative of the preferred embodiments of the present application and are not intended to limit the scope of the present application, and various modifications and improvements made by those skilled in the art to the technical solutions of the present application should fall within the protection scope defined by the claims of the present application without departing from the design spirit of the present application.
Claims (10)
1. The utility model provides a cell dynamic circumference stretching device of real-time fluorescence imaging for use with confocal microscope is supporting, its characterized in that: the confocal microscope comprises a shell (1), wherein the top surface of the shell (1) is open, the size of the shell (1) is matched with the size of a placement area of the confocal microscope, and a sample (2) and a stretching mechanism are arranged in the shell (1);
the sample (2) is integrally cast and formed, a placing part is arranged on the sample (2), the placing part is matched with the eyepiece of the confocal microscope in size, and a sealing part is detachably connected to the placing part;
the stretching mechanism comprises a pressing ring (3) and a driving part, the driving part is in transmission connection with the pressing ring (3), the size of the inner ring of the pressing ring (3) is matched with the size of an eyepiece of the confocal microscope, a plurality of connecting rod assemblies are circumferentially arranged on the side wall of the pressing ring (3), and the bottom ends of the connecting rod assemblies are spliced with the sample (2);
an opening (17) is formed in the inner bottom surface of the shell (1), the circle center of the sample (2) corresponds to the circle center of the opening (17), and the size of the opening (17) is larger than that of an objective lens of the confocal microscope.
2. The real-time fluorescence imaging cell dynamic circumferential stretching device according to claim 1, wherein: the length of the shell (1) is 240mm, the width is 120mm, and the height is 55.49mm.
3. The real-time fluorescence imaging cell dynamic circumferential stretching device according to claim 1, wherein: the placing part comprises four cell storage tanks (4) with the same size, and the four cell storage tanks (4) are symmetrically arranged on the top surface of the sample (2) in pairs.
4. A real time fluorescence imaging cell dynamic circumferential stretching apparatus according to claim 3, wherein: the diameters of the cell storage tanks (4) are 18mm, the distance between the circle center diameters of any two adjacent cell storage tanks (4) is 15-22mm, and the diameter of the circle tangent line of the whole of the four cell storage tanks (4) is 33-40mm.
5. A real time fluorescence imaging cell dynamic circumferential stretching apparatus according to claim 3, wherein: the distance between the bottom surface of the cell storage groove (4) and the bottom surface of the sample (2) is 1mm.
6. A real time fluorescence imaging cell dynamic circumferential stretching apparatus according to claim 3, wherein: the sealing part comprises an annular clamping groove (5), the annular clamping groove (5) is formed in the top surface of the sample (2), the annular clamping groove (5) is used for coating four cell storage grooves (4), a transparent culture dish cover (6) is detachably connected to the annular clamping groove (5), and the culture dish cover (6) is made of PDMS materials with the ratio of 1:1.5-1:10.
7. The real-time fluorescence imaging cell dynamic circumferential stretching device according to claim 1, wherein: the diameter of the inner ring of the compression ring (3) is 40-55mm.
8. The real-time fluorescence imaging cell dynamic circumferential stretching device according to claim 1, wherein: the connecting rod assembly comprises an upper connecting rod (7), the top end of the upper connecting rod (7) is hinged with the side wall of the pressing ring (3), the bottom end of the upper connecting rod (7) is hinged with a lower connecting rod (8), and the bottom end of the lower connecting rod (8) is spliced with the sample (2).
9. The real-time fluorescence imaging cell dynamic circumferential stretching device according to claim 1, wherein: the drive part comprises two servo motors (9), the two servo motors (9) are respectively located on two sides of the sample (2), the servo motors (9) are fixedly installed on the inner bottom surface of the shell (1), a lead screw (10) is fixedly connected with an output shaft of each servo motor (9), a push plate (11) is connected to the lead screw (10) in a transmission mode, two connecting rods (12) are fixedly connected between the push plates (11), two connecting rings (13) are symmetrically fixedly connected to the top surface of the compression ring (3), and the two connecting rods (12) are respectively arranged in the two connecting rings (13) in a penetrating mode.
10. The real-time fluorescence imaging cell dynamic circumferential stretching device according to claim 9, wherein: an external speed regulation display (14) is fixedly connected to one side of the shell (1), a driving plate (15) and a control plate (16) are fixedly installed in the external speed regulation display (14), the driving plate (15) is electrically connected with the control plate (16), the control plate (16) is electrically connected with the external speed regulation display (14), and the driving plate (15) is electrically connected with the two servo motors (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310555350.3A CN116754528B (en) | 2023-05-17 | 2023-05-17 | Cell dynamic circumferential stretching device for real-time fluorescence imaging |
Applications Claiming Priority (1)
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| CN1932510A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Cell tensile loader |
| KR20190046751A (en) * | 2019-04-29 | 2019-05-07 | 전남대학교산학협력단 | Microscope mounting type cell stretching apparatus |
| US20190390152A1 (en) * | 2017-02-15 | 2019-12-26 | Ifom - Fondazione Istituto Firc Di Oncologia Molecolare | Cell stretching device |
| CN113322154A (en) * | 2021-05-24 | 2021-08-31 | 天津理工大学 | Mechanical loading device for applying uniform circumferential quasi-static/dynamic cyclic strain field |
| CN215512246U (en) * | 2021-06-18 | 2022-01-14 | 惠州英特智能设备有限公司 | Gauze mask processing flattening device |
| CN115584322A (en) * | 2022-11-15 | 2023-01-10 | 太原理工大学 | Three-dimensional dynamic cell volume regulating and controlling device |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1932510A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Cell tensile loader |
| US20190390152A1 (en) * | 2017-02-15 | 2019-12-26 | Ifom - Fondazione Istituto Firc Di Oncologia Molecolare | Cell stretching device |
| KR20190046751A (en) * | 2019-04-29 | 2019-05-07 | 전남대학교산학협력단 | Microscope mounting type cell stretching apparatus |
| CN113322154A (en) * | 2021-05-24 | 2021-08-31 | 天津理工大学 | Mechanical loading device for applying uniform circumferential quasi-static/dynamic cyclic strain field |
| CN215512246U (en) * | 2021-06-18 | 2022-01-14 | 惠州英特智能设备有限公司 | Gauze mask processing flattening device |
| CN115584322A (en) * | 2022-11-15 | 2023-01-10 | 太原理工大学 | Three-dimensional dynamic cell volume regulating and controlling device |
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