CN115584322B - Cell three-dimensional dynamic volume regulating and controlling device - Google Patents

Cell three-dimensional dynamic volume regulating and controlling device Download PDF

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CN115584322B
CN115584322B CN202211428326.5A CN202211428326A CN115584322B CN 115584322 B CN115584322 B CN 115584322B CN 202211428326 A CN202211428326 A CN 202211428326A CN 115584322 B CN115584322 B CN 115584322B
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cell
storage groove
motor
inner ring
sample
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CN115584322A (en
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张全有
张强
兰敏华
郭华庆
陈维毅
卫小春
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Taiyuan University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

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Abstract

The invention discloses a cell three-dimensional dynamic volume regulating device, which comprises: the device comprises a die unit, a stretching unit and a control driving unit, wherein the die unit comprises a pouring die for pouring a formed sample, a cell storage groove is formed in an inner ring of the sample, a plurality of single cell grooves are formed in the cell storage groove, and a plurality of insertion holes are formed in the periphery of an outer ring of the sample; the stretching unit comprises a mounting assembly, a plurality of arc-shaped rotating blades are movably connected in the mounting assembly, the plurality of rotating blades are arranged in a diaphragm shape, stretching rods are fixedly connected to the bottom surface of the inner ring end of each rotating blade, and the plurality of stretching rods are arranged in one-to-one correspondence with the plurality of jacks and are fixedly connected with the same; the control driving unit comprises a motor and a control assembly, wherein the motor is electrically connected with the control assembly, and an output shaft of the motor is in transmission connection with the mounting assembly through the transmission assembly.

Description

Cell three-dimensional dynamic volume regulating and controlling device
Technical Field
The invention relates to the field of biomedical engineering, in particular to a cell three-dimensional dynamic volume regulating device.
Background
Cells exist in vivo in complex three-dimensional matrix microenvironments that regulate cell proliferation, differentiation, and function by sensing and responding to various stimuli such as biochemical and mechanical stresses. In recent years, it has been deeply discovered that the extracellular matrix microenvironment as a direct cell contact region has important regulation and control effects on cell mechanics and biological behaviors, and that the change of physical characteristics of the matrix microenvironment is closely related to growth, development and disease occurrence. Matrix microenvironment physical properties include matrix elasticity, viscoelasticity, single cell geometry, dynamic tensile and compressive strain stimuli, and the like.
With the continuous and deep research of cell mechanics biology, people gradually realize that the research of the cell behavior of single cells in the three-dimensional matrix microenvironment has more physiological and practical significance. At the same time, numerous studies have demonstrated that single cell volume directly affects cell behavior and fate, including cell migration, differentiation, and apoptosis, through specific signaling pathways. Such as increased cellular rigidity caused by decreased cell volume due to outflow of cellular water, and eventually induces stem cells to become preosteoblasts. In addition, cell volume regulation affects not only cell function but also gene expression, metabolic activity and degenerative diseases such as chondrocyte hypertrophy and the like. Therefore, engineering a cell culture platform or device that regulates single cell volume in three dimensions is critical to reveal the molecular mechanisms by which dynamic volume changes in the matrix regulate cell behavior, function, and fate.
The existing cell stretching and compressing devices are mostly limited on two-dimensional plane strain of population cells, and the diversity of cell stretching and compressing modes is not comprehensively considered, and the importance of single-cell three-dimensional volume regulation and control and the real-time performance of microscope observation under the practical research meaning based on cell dynamic strain are not considered. With the development of biomedical engineering, it is far from sufficient to focus the eye on the axial stretching and compression of cells in a population.
Disclosure of Invention
The invention aims to provide a cell three-dimensional dynamic volume regulating device, which aims to solve or improve at least one of the technical problems.
In order to achieve the above object, the present invention provides the following solutions: the invention provides a cell three-dimensional dynamic volume regulating device, which comprises:
the die unit comprises a pouring die for pouring a formed sample, the inner ring of the sample is provided with a cell storage groove, a plurality of single cell grooves are formed in the cell storage groove, and a plurality of insertion holes are formed in the periphery of the outer ring of the sample;
the stretching unit comprises a mounting assembly, a plurality of arc-shaped rotating blades are movably connected in the mounting assembly, the plurality of rotating blades are arranged in a diaphragm shape, stretching rods are fixedly connected to the bottom surface of the inner ring end of each rotating blade, and the plurality of stretching rods are arranged in one-to-one correspondence with the plurality of jacks and are fixedly connected with the jacks;
the control driving unit comprises a motor and a control assembly, wherein the motor is electrically connected with the control assembly, and an output shaft of the motor is in transmission connection with the installation assembly through a transmission assembly.
Preferably, the pouring die comprises an outer barrel with one open end, a storage groove forming block is movably connected to the center of an inner end face of the outer barrel away from the open end of the outer barrel, the storage groove forming block is arranged corresponding to the cell storage groove, a photoetching silicon plate is arranged on the top surface of the storage groove forming block, and a plurality of protrusions on the surface of the photoetching silicon plate are arranged corresponding to a plurality of single cell grooves one by one.
Preferably, the inner end surface of the outer barrel far away from the open end of the outer barrel is fixedly connected with a plurality of jack forming rods in the circumferential direction, the jack forming rods are located on the outer side of the storage groove forming block, one end of the jack forming rods far away from the storage groove forming block extends out of the open end of the outer barrel, and the jack forming rods are in one-to-one correspondence with the jack positions.
Preferably, the open end of the outer cylinder is detachably connected with a sealing cover, a pouring opening is formed in the center of the sealing cover, a gap is formed between the pouring opening and the storage groove forming block, a plurality of through holes are formed in the periphery of the outer ring of the sealing cover, and the through holes are in one-to-one correspondence with the jack forming rods and are spliced.
Preferably, the installation component includes inner ring and outer loop, a plurality of rotor blade's outer lane end with the outer loop rigid coupling, the inner ring bottom surface circumference articulates there is a plurality of connecting rod, and a plurality of the connecting rod with a plurality of rotor blade one-to-one sets up, just the connecting rod keep away from the inner ring one end with rotor blade's top surface articulates.
Preferably, the transmission assembly comprises a swing arm, one end of the swing arm is fixedly connected with the inner ring, one end of a crank is hinged to the other end of the swing arm, the other end of the crank is fixedly connected with the top surface of an eccentric wheel, and the bottom surface of the eccentric wheel is fixedly connected with the output shaft of the motor.
Preferably, the control assembly comprises a controller, wherein the controller is electrically connected with the motor, and the controller is provided with a switch button, an stepless speed regulating knob and a pause button.
The invention discloses the following technical effects:
according to the invention, a casting mold is used for casting and forming the sample, so that a cell storage groove in the sample forms a plurality of single cell grooves for storing each single cell, after the cell is attached to the wall, a motor drives a transmission assembly to rotate a plurality of rotating blades, so that each item of the sample below the stretching rod is driven to be uniformly deformed, and the volume of the single cell groove in the cell storage groove is uniformly expanded.
And the motor drives the transmission assembly to rotate the plurality of rotating blades to a required angle, so that each item of the sample below the stretching rod is driven to be uniformly deformed, and the single cell groove in the cell storage groove is uniformly expanded in volume. At this time, the cells are planted into the single cell groove in the cell storage groove, after the cells are attached to the wall, the rotary motor drives the transmission assembly to enable the plurality of rotary blades to rotate, then the lower sample is restored to the original shape, and the single cell groove in the cell storage groove is uniformly compressed in volume.
Thereby realizing the three-dimensional volume regulation and control of each single cell in the single cell groove under the real-time observation of the confocal microscope. The method can be directly applied to experiments such as immune cell staining and calcium imaging, and can realize accurate quantitative analysis of three-dimensional cell morphology, focal adhesion protein, protein distribution, functional factor fluorescence intensity and calcium concussion in a dynamic stimulation process through real-time observation of a confocal microscope.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic perspective view of the minimum state of the present invention;
FIG. 2 is a schematic perspective view of the present invention in a maximum state;
FIG. 3 is a schematic perspective view of a casting mold according to the present invention;
FIG. 4 is a schematic perspective view of the interior of the casting mold according to the present invention;
FIG. 5 is a schematic diagram of a lithographic silicon plate according to the present invention;
in the figure: 1. a sample; 2. pouring a mold; 3. a cell storage tank; 5. a jack; 6. rotating the blade; 7. a stretching rod; 8. a motor; 9. an inner ring; 10. an outer ring; 11. a connecting rod; 12. swing arms; 13. a crank; 14. an eccentric wheel; 15. a controller; 16. a switch button; 17. a stepless speed regulating knob; 18. a pause button; 21. an outer cylinder; 22. a storage tank forming block; 23. photoetching a silicon plate; 24. a jack forming rod; 25. a cover; 26. a sprue gate; 27. and a through hole.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Example 1
Referring to fig. 1 to 5, the present embodiment provides a three-dimensional dynamic volume control device for cells, comprising:
the die unit comprises a pouring die 2 for pouring and forming a sample 1, wherein the inner ring of the sample 1 is provided with a cell storage groove 3, a plurality of single cell grooves are formed in the cell storage groove 3, and a plurality of insertion holes 5 are formed in the circumferential direction of the outer ring of the sample 1;
the stretching unit comprises a mounting assembly, a plurality of arc-shaped rotating blades 6 are movably connected in the mounting assembly, the plurality of rotating blades 6 are arranged in a diaphragm shape, stretching rods 7 are fixedly connected to the bottom surface of the inner ring end of the rotating blades 6, and the plurality of stretching rods 7 are arranged in one-to-one correspondence with the plurality of jacks 5 and fixedly connected with the same;
the control driving unit comprises a motor 8 and a control assembly, wherein the motor 8 is electrically connected with the control assembly, and an output shaft of the motor 8 is in transmission connection with the installation assembly through a transmission assembly.
According to the invention, the pouring die 2 is used for pouring and forming the sample 1, so that the cell storage groove 3 in the sample 1 forms a plurality of single cell grooves for storing each single cell, after the obtained cells are attached to the wall, the motor 8 drives the transmission assembly to rotate the plurality of rotating blades 6, the stretching rod 7 is driven to squeeze each item of the sample 1 below to deform uniformly, and the single cell grooves in the cell storage groove 3 expand uniformly in volume.
Similarly, the motor 8 drives the transmission assembly to rotate the plurality of rotating blades 6 to a required angle, and drives the stretching rod 7 to squeeze each item of the lower sample 1 to deform uniformly, so that the single cell groove in the cell storage groove 3 expands uniformly in volume. At this time, the cells are planted into the single cell groove in the cell storage groove 3, after the cells are attached, the rotary motor 8 drives the transmission assembly to enable the plurality of rotary blades 6 to rotate, then the lower sample 1 is restored to the original shape, and the single cell groove in the cell storage groove 3 is uniformly compressed in volume.
Thereby realizing the three-dimensional volume regulation and control of each single cell in the single cell groove under the real-time observation of the confocal microscope.
Further, the torque of the motor 8 is 25 kg.m, and the motor can rotate at a constant speed of 1r/min-10r/min or stop at a certain place.
Further, the number of the rotor blades 6 is preferably 16, improving the uniformity of each stretching.
Further, the cell storage tank 3 in the sample 1 of the present invention preferably has an effect of expanding the entire diameter by 20 mm.
In a further optimized scheme, the pouring die 2 comprises an outer barrel 21 with one open end, a storage groove forming block 22 is movably connected to the center of the inner end surface of the outer barrel 21 far away from the open end of the outer barrel, the storage groove forming block 22 is arranged corresponding to the cell storage groove 3, the top surface of the storage groove forming block 22 is provided with a photoetching silicon plate 23, and a plurality of bulges on the surface of the photoetching silicon plate 23 are arranged corresponding to a plurality of single cell grooves one by one; the inner end surface of the outer cylinder 21 far away from the open end of the outer cylinder is fixedly connected with a plurality of jack forming rods 24 in the circumferential direction, the jack forming rods 24 are located outside the storage groove forming block 22, one end of the jack forming rods 24 far away from the storage groove forming block 22 extends out of the open end of the outer cylinder 21, and the jack forming rods 24 and the jack 5 are arranged in a one-to-one correspondence mode.
The cell storage tank 3, the photoetching silicon plate 23 and the plurality of jack forming rods 24 are arranged, so that the sample cast by the casting mold 2 is provided with the cell storage tank 3, the single cell tank and the jacks 5.
Further, the size of the protrusions of the photoetching silicon plate 23 can only accommodate single cells, the size is in the micron order, and the photoetching silicon plate 23 is preferably detachably connected to the storage tank forming block 22 in a sticking mode.
Further, a groove (not shown in the figure) with a size matching with that of the storage groove forming block 22 is formed in the center of the end wall of the outer barrel 21 far away from the open end of the outer barrel, a through hole (not shown in the figure) is formed in the center of the groove, a fixing rod is fixedly connected to the end face of the storage groove forming block 22 far away from the photoetching silicon plate 23, and when the storage groove forming block 22 is connected with the outer barrel 21, the fixing rod penetrates through the through hole and the storage groove forming block 22 is placed in the groove, so that the storage groove forming block 22 can be stabilized in the outer barrel 21.
Further optimizing scheme, the open end of urceolus 21 can be dismantled and be connected with closing cap 25, and the center department of closing cap 25 has seted up pouring gate 26, has the clearance between pouring gate 26 and the storage tank shaping piece 22, and the outer lane circumference of closing cap 25 has seted up a plurality of through-hole 27, and a plurality of through-hole 27 and a plurality of jack shaping pole 24 one-to-one set up and peg graft.
Pouring into the pouring die 2 is facilitated by providing the pouring gate 26, and the cover 25 is connected with the outer cylinder 21 at the time of pouring by providing the through hole 27.
Further, sample 1 is PDMS, and the pouring ratio at the pouring port 26 is 1:20, the modulus of elasticity and the density of which were measured by selecting a relatively flat sample 1 and by a tensile compression test.
Further optimizing scheme, the installation component includes inner ring 9 and outer loop 10, and the outer lane end and the outer loop 10 rigid coupling of a plurality of rotor blade 6, and inner ring 9 bottom surface circumference articulates there is a plurality of connecting rod 11, and a plurality of connecting rod 11 and a plurality of rotor blade 6 one-to-one setting, and the one end that inner ring 9 was kept away from to connecting rod 11 articulates with rotor blade 6's top surface.
The inner ring 9 is driven to rotate through the transmission component, the outer ring ends of a plurality of rotating blades 6 are fixed by the outer ring 10, and the rotating blades 6 can be rotated by the connecting rods 11 driven by the inner ring 9, so that the stretching or shrinking control of a plurality of stretching rods 7 on the sample 1 is realized.
Further, the outer ring 10 is fixedly connected with the motor 8 through a bracket, and is used for fixing the position of the outer ring 10.
Further optimizing scheme, drive assembly includes swing arm 12, and swing arm 12 one end and inner ring 9 rigid coupling, the other end of swing arm 12 articulates the one end that has crank 13, and the other end rigid coupling of crank 13 has the top surface of eccentric wheel 14, the bottom surface of eccentric wheel 14 and the output shaft rigid coupling of motor 8.
The output shaft of the motor 8 drives the swing arm eccentric wheel 14 to rotate, so that the crank 13 drives the swing arm 12 to move, and further the swing arm 12 drives the inner ring 9 to rotate.
Further optimizing scheme, the control assembly includes the controller 15, and controller 15 and motor 8 electric connection have shift knob 16, stepless speed control knob 17 and pause button 18 on the controller 15.
The stepless speed regulating knob 17 has the function of changing the rotation speed of the output shaft of the motor 8, and the pause button 18 has the function of facilitating the static observation of the cell state under a certain strain state.
Furthermore, the size of the device is designed according to the size of the Leka confocal microscope, so that the effect of real-time observation can be achieved.
The using method comprises the following steps:
casting and forming, namely installing a storage groove forming block 22 in the middle of a casting die 2, tightly adhering a photoetching silicon plate 23 to the surface of the storage groove forming block 22, and pouring the silicon plate into a casting hole 26 according to the proportion of 1: after 20 PDMS glue, demoulding to form a sample 1 with a cell storage tank 3 and a single cell tank, wherein the single cell tank can accommodate single cells, so that single cell culture is realized;
inoculating cells, carrying out aseptic treatment on the device, inoculating the cells in a single cell groove of the formed sample 1, and placing the single cell groove into an incubator for culture;
in the experiment, the motor 8 is connected with the controller 15, the whole device is moved to the position under the leica confocal microscope and is placed at a proper place, so that the lens can be aligned with the cell storage groove 3 in the sample 1 to observe cells;
the motor 8 is controlled by the controller 15 to rotate the motor output shaft, drives the eccentric wheel 14 to swing the crank 13, and further rotates the inner ring 9 through the swing arm 12. The inner ring 9 rotates the rotating blades 6 through the connecting rods 11, so that the stretching rod 7 is driven to deform the lower sample 1 from the minimum state to the maximum state, and the cells in the cell storage tank 3 are uniformly stretched in all directions. At this time, the experimenter can observe the dynamics of cell volume expansion in real time under a confocal microscope.
Similarly, the motor 8 is controlled by the controller 15 to rotate the motor output shaft, drive the eccentric wheel 14 to swing the crank 13, and further rotate the inner ring 9 through the swing arm 12. The inner ring 9 rotates the rotating blade 6 to a required angle through the connecting rod 11, and drives the stretching rod 7 to squeeze each item of the sample 1 below to deform uniformly, so that the single cell groove in the cell storage groove 3 expands uniformly in volume. At this time, the cells are planted into the single cell groove in the cell storage groove 3, after the cells are attached, the rotary motor 8 drives the transmission assembly to enable the plurality of rotary blades 6 to rotate, then the lower sample 1 is restored to the original shape, and the single cell groove in the cell storage groove 3 is uniformly compressed in volume. The experimenter can observe the dynamics of cell volume compression in real time under a confocal microscope.
In addition, the motor 8 of the present invention can reciprocate at a certain rate, and pressing the pause button 18 can be stopped at an arbitrary position.
Example two
The difference between this embodiment and the first embodiment is that, when pouring and molding the sample 1, the surface of the storage tank molding block 22 is not tightly attached to a photolithographic silicon plate 23, so that the cell storage tank 3 is not provided with a single cell tank after molding the sample 1, and thus, the cell storage tank 3 can be used for culturing the population cells, and further, the stretching rod 7 is driven to squeeze the sample 1 below to generate uniform strain, and the uniform strain of each plane of the population cells in the tank is realized under the real-time observation of a confocal microscope.
In the description of the present invention, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.

Claims (5)

1. Cell three-dimensional dynamic volume regulation and control device, characterized by comprising:
the device comprises a die unit, wherein the die unit comprises a pouring die (2) for pouring a formed sample (1), a cell storage groove (3) is formed in the inner ring of the sample (1), a plurality of single-cell grooves are formed in the surface of the cell storage groove (3), and a plurality of jacks (5) are circumferentially formed in the outer ring of the sample (1);
the stretching unit comprises a mounting assembly, a plurality of arc-shaped rotating blades (6) are movably connected in the mounting assembly, the plurality of rotating blades (6) are arranged in a diaphragm shape, stretching rods (7) are fixedly connected to the bottom surface of the inner ring end of each rotating blade (6), and the plurality of stretching rods (7) are arranged in one-to-one correspondence with the plurality of jacks (5) and fixedly connected;
the control driving unit comprises a motor (8) and a control assembly, wherein the motor (8) is electrically connected with the control assembly, and an output shaft of the motor (8) is in transmission connection with the installation assembly through a transmission assembly;
the pouring die (2) comprises an outer cylinder (21) with one open end, a storage groove forming block (22) is movably connected to the center of an inner end surface of the outer cylinder (21) far away from the open end of the outer cylinder, the storage groove forming block (22) is arranged corresponding to the cell storage groove (3), a photoetching silicon plate (23) is arranged on the top surface of the storage groove forming block (22), and a plurality of bulges on the surface of the photoetching silicon plate (23) are arranged in one-to-one correspondence with a plurality of single cell grooves;
the inner end surface circumference rigid coupling that urceolus (21) kept away from its open end has a plurality of jack shaping pole (24), and a plurality of jack shaping pole (24) are located the storage tank shaping piece (22) outside, a plurality of jack shaping pole (24) are kept away from the one end of storage tank shaping piece (22) stretches out the open end of urceolus (21), and a plurality of jack shaping pole (24) and a plurality of jack (5) position one-to-one sets up.
2. The cell three-dimensional dynamic volume control device of claim 1, wherein: the novel energy-saving storage tank is characterized in that a sealing cover (25) is detachably connected to the open end of the outer cylinder (21), a pouring opening (26) is formed in the center of the sealing cover (25), a gap is formed between the pouring opening (26) and the storage tank forming block (22), a plurality of through holes (27) are formed in the periphery of the outer ring of the sealing cover (25), and the through holes (27) are in one-to-one correspondence with the jack forming rods (24) and are spliced.
3. The cell three-dimensional dynamic volume control device of claim 1, wherein: the installation component includes inner ring (9) and outer loop (10), a plurality of outer lane end of rotor blade (6) with outer loop (10) rigid coupling, inner ring (9) bottom surface circumference articulates there is a plurality of connecting rod (11), a plurality of connecting rod (11) and a plurality of rotor blade (6) one-to-one sets up, just connecting rod (11) are kept away from the one end of inner ring (9) with the top surface of rotor blade (6) articulates.
4. A cell three-dimensional dynamic volume control device according to claim 3, wherein: the transmission assembly comprises a swing arm (12), one end of the swing arm (12) is fixedly connected with the inner ring (9), one end of a crank (13) is hinged to the other end of the swing arm (12), the other end of the crank (13) is fixedly connected with the top surface of an eccentric wheel (14), and the bottom surface of the eccentric wheel (14) is fixedly connected with the output shaft of the motor (8).
5. The cell three-dimensional dynamic volume control device of claim 1, wherein: the control assembly comprises a controller (15), wherein the controller (15) is electrically connected with the motor (8), and the controller (15) is provided with a switch button (16), an stepless speed regulating knob (17) and a pause button (18).
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