CN116751654A - Targeted microorganism culture equipment for culture tail water treatment and culture method thereof - Google Patents
Targeted microorganism culture equipment for culture tail water treatment and culture method thereof Download PDFInfo
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- CN116751654A CN116751654A CN202311035946.7A CN202311035946A CN116751654A CN 116751654 A CN116751654 A CN 116751654A CN 202311035946 A CN202311035946 A CN 202311035946A CN 116751654 A CN116751654 A CN 116751654A
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- 244000005700 microbiome Species 0.000 title claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 238000012136 culture method Methods 0.000 title abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 13
- 238000012216 screening Methods 0.000 claims abstract description 5
- 238000009413 insulation Methods 0.000 claims description 37
- 239000010425 asbestos Substances 0.000 claims description 19
- 229910052895 riebeckite Inorganic materials 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 14
- 239000011521 glass Substances 0.000 claims description 13
- 230000007246 mechanism Effects 0.000 claims description 12
- 238000012165 high-throughput sequencing Methods 0.000 claims description 6
- 238000009360 aquaculture Methods 0.000 claims description 5
- 244000144974 aquaculture Species 0.000 claims description 5
- 238000005516 engineering process Methods 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 3
- 230000017525 heat dissipation Effects 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 claims description 2
- 230000004927 fusion Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000011261 inert gas Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241000736262 Microbiota Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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Abstract
The invention relates to the technical field of targeted microorganism culture, in particular to targeted microorganism culture equipment for culture tail water treatment and a culture method thereof, wherein the targeted microorganism culture equipment comprises a heat-insulating outer frame, a warm air blower, a plurality of heat-insulating incubator and a plurality of brake screws; the invention provides places for strain culture and separates and isolates strains under different culture conditions to avoid strain fusion, and the invention changes the temperature of a culture dish in each heat-insulating culture box by a plurality of secondary pipelines, a warm air blower, a plurality of heat-insulating culture boxes and a plurality of braking screws to detect the temperature of the culture condition required by culturing target strains in the culture dish and assist in screening the culture condition required by the target strains.
Description
Technical Field
The invention relates to the technical field of targeted microorganism culture, in particular to targeted microorganism culture equipment for culture tail water treatment and a culture method thereof.
Background
In the existing aquaculture, a large amount of organic matters such as residual bait and feces for aquaculture, the respiration of aquatic organisms and the decomposition of organic matters generate carbon dioxide, the respiration of cultured animals, the consumption of a large amount of oxygen by residual bait and excrement and the like, the concentration of total nitrogen and total phosphorus in water bodies are excessive, harmful gases and harmful substances generated by the decomposition of sediments, the eutrophication of aquaculture wastewater and the like can pollute the water bodies, so that the discharged aquaculture tail water needs to be treated to prevent the water environment from being polluted.
Tail water treatment methods can be divided into three types of physical methods, chemical methods and biological methods, the actual treatment process is often a combination of the three methods, and microorganisms are main participants in degradation and conversion of organic pollutants in the cultivation wastewater treatment process.
However, traditional non-targeted microorganisms and new high-throughput culture methods, such as a culture histology platform that relies on the use of a variety of media and high-throughput screening methods, have resulted in many new and previously uncultured lineages being brought into culture. This approach is often labor and resource intensive and does not necessarily capture the particular target microbiota of interest in the community. Therefore, it is important to obtain specific strains through a targeted isolated culture technology. In addition, in the process of targeted separation, strains are required to be cultivated and screened on a large scale under different conditions, the strains are classified and disordered due to various cultivated strain types, the strains are inconvenient to take, and the hidden danger of strain fusion exists due to the collision of the culture dishes, so that the strain cultivation quality is affected. In view of this, we propose a targeted microorganism culture apparatus for cultivation tail water treatment and a cultivation method thereof.
Disclosure of Invention
In order to overcome the defects, the invention provides targeted microorganism culture equipment for treating the culture tail water and a culture method thereof.
The technical scheme of the invention is as follows:
the cultivation tail water treatment is with target microorganism culture apparatus includes:
the heat insulation outer frame is internally provided with a rectangular inner cavity which is communicated with the heat insulation outer frame from front to back;
a warm air blower arranged at the top end of the heat insulation outer frame;
the heat insulation cultivation boxes are respectively arranged on the front side and the rear side of the rectangular inner cavity in a matrix manner, the warm air blower is arranged between the heat insulation cultivation boxes on the two sides and used for manufacturing cool air between the heat insulation cultivation boxes on the two sides, the heat insulation cultivation boxes comprise cultivation dishes for cultivating strains, the rear ends of the heat insulation cultivation boxes are formed by inlet rear plates, the inlet rear plates comprise inlet valves, three sliding plates are arranged in the inlet valves in a left-right moving manner, one side of each sliding plate is provided with a fixing block, and threaded holes are formed in the fixing blocks;
the brake screw rod is arranged on the heat insulation outer frame and is composed of a first motor, a second round rod is arranged at the output end of the first motor through a coupler, the second round rod is arranged in the threaded hole, a plurality of external threads are arranged on the second round rod, and the external threads are meshed with internal threads in the threaded hole.
Preferably, a first side plate and a second side plate which are parallel to each other are arranged on two sides of the inlet rear plate, a top plate and a heating bottom plate are respectively arranged on the upper end and the lower end of the inlet rear plate, and a box door is arranged on the front side of the heating bottom plate, the first side plate, the second side plate and the top plate.
Preferably, the inlet rear plate further comprises a first sliding plate and a second sliding plate which are parallel to each other, the third sliding plate is slidably arranged between the first sliding plate and the second sliding plate, the first sliding plate and the second sliding plate are respectively connected with the first side plate, the second side plate and the top plate, and the air inlet valve is arranged on the first sliding plate and the second sliding plate.
Preferably, the second sliding plate is provided with a sliding groove communicated with the air inlet valve, and the fixed block is arranged in the sliding groove.
Preferably, the heating bottom plate comprises a rectangular plate, the rectangular plate respectively with curb plate one curb plate two slide one slide two interconnect, be equipped with the cavity in the rectangular plate, install the trunk line in the cavity, be equipped with a plurality of follow pipeline of mutual intercommunication on the trunk line, every all installs a plurality of outlet ducts that are annular equidistant range from the pipeline, slewing mechanism is installed to the top of rectangular plate, a plurality of bracing piece is installed to slewing mechanism's below, and slewing mechanism's top is equipped with the ring, the asbestos net is installed to the below of going up the ring, every the top of outlet duct all stretches into the below of asbestos net.
Preferably, the culture dish is arranged on the asbestos net, the end of the culture dish is arranged in the upper ring, a glass cover is arranged on the culture dish for anaerobic bacteria, and a clamping groove is formed in the inner side of the side plate II.
Preferably, the box door is composed of a front plate, the front plate is rotatably installed on the front side of the first side plate, a heat dissipation hole and a mark are formed in the upper end of the front plate, and a glass observation window is arranged below the front plate.
The method for culturing the target microorganism for treating the culture tail water comprises the following steps:
step one, confirming a target strain;
and step two, screening culture conditions.
Further, the specific operation of the first step is as follows: firstly, extracting culture tail water as a microorganism detection sample, then detecting the types and the relative abundance of microorganisms in the sample by a second-generation and third-generation high-throughput sequencing technology, and determining whether target microorganisms exist in the sample.
Further, the specific operation of the second step is as follows: step one, confirming a sample containing target microorganisms, respectively culturing, separating, purifying and the like through culture dishes with anaerobic or aerobic culture, different formulas and temperatures to find target strains, carrying out high-throughput sequencing identification on the purified target strains, and determining the target strains from the gene level.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention provides places for strain culture and separates and isolates strains under different culture conditions through the plurality of heat-insulating incubators, thereby avoiding strain fusion.
2. The invention is used for changing the temperature of a culture dish in each heat-insulating incubator by a plurality of secondary pipelines, a warm air blower, a plurality of heat-insulating incubators and a plurality of braking screws, thereby detecting the temperature of culture conditions required by culturing target strains in the culture dish and assisting in screening the target strains.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view, partially in section, of the present invention;
FIG. 3 is a schematic view showing a connection structure of a brake screw and a heat insulation outer frame according to the present invention;
FIG. 4 is a schematic diagram of the structure of the heat-insulating incubator of the present invention;
FIG. 5 is a schematic view of an exploded view of the insulated incubator of the present invention;
FIG. 6 is a schematic view of the structure of the door of the present invention;
FIG. 7 is a schematic diagram of a rectangular twist structure according to the present invention;
FIG. 8 is a schematic view, partially in section, of a heated soleplate of the present invention;
FIG. 9 is a schematic view of a rear plate structure of the present invention;
FIG. 10 is a schematic view of a partial structure of the heating base plate and the rear plate of the present invention;
FIG. 11 is a schematic view of a rotary mechanism according to the present invention;
fig. 12 is a side view of a second side plate of the present invention.
In the figure:
1. a heat insulating outer frame;
2. a warm-air drier;
3. a heat-insulating incubator; 31. a first side plate; 32. a top plate; 33. a second side plate; 34. a door; 341. a front plate; 342. a heat radiation hole; 343. marking; 344. a rectangular turn knob; 345. a glass viewing window; 346. a first circular rod; 347. a rectangular bar; 35. heating the bottom plate; 351. a rectangular plate; 352. a cavity; 353. a slave pipe; 354. a main pipe; 357. an upper circular ring; 358. an asbestos mesh; 36. a rear plate; 361. a first sliding plate; 362. a second slide plate; 363. a third slide plate; 364. a fixed block; 365. a threaded hole; 366. a chute; 367. an intake valve; 37. a clamping groove; 38. a culture dish; 39. a glass cover;
4. braking the screw; 41. a first motor; 42. a second circular rod; 43. an external thread;
5. a water outlet hole;
6. a wrap cover; 61. ventilation holes;
7. a connection cover;
8. an air outlet pipe;
9. a rotating mechanism; 91. a bottom support ring; 92. an annular groove; 93. a rotating ring; 94. convex teeth; 95. a second motor; 96. a gear.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Referring to fig. 1-12, the present invention is described in detail by the following embodiments:
the utility model provides a breed tail water treatment is with target microorganism culture apparatus, including thermal-insulated frame 1, be equipped with the rectangle inner chamber that link up from front to back in the thermal-insulated frame 1, a plurality of thermal-insulated incubator 3 are installed to the rectangle inner chamber, a plurality of thermal-insulated incubator 3 are not only used for providing the place for the bacterial cultivation, still be used for separating the bacterial of different cultivation conditions and keep apart, avoided the bacterial to fuse, every fifty thermal-insulated incubator 3 is a set of, every ten thermal-insulated incubator 3 is installed in the rectangle inner chamber in proper order horizontal arrangement, i.e. every group thermal-insulated incubator 3 divide into five rows and install in the rectangle inner chamber, two sets of thermal-insulated incubator 3 are located the front and back both sides in rectangle inner chamber respectively, have the clearance between two sets of thermal-insulated incubator 3, the top of two sets of thermal-insulated incubator 3 is equipped with electric fan heater 2, electric fan heater 2 fixed mounting is in the top of thermal-insulated frame 1.
What needs to be supplemented is, be equipped with down the apopore 5 on the bottom surface in the rectangle inner chamber, electric fan heater 2 is used for providing steam, and electric fan heater 2's steam export down to be located between two sets of thermal-insulated incubator 3, electric fan heater 2 produces steam can be full of between two sets of thermal-insulated incubator 3 and thermal-insulated frame 1, partial hot air meets cold, there is partial gas liquefaction, the gaseous apopore 5 of below that can follow of liquefaction flows out, not only electric fan heater 2 and thermal-insulated incubator 3 still are equipped with the fan that can increase the steam and flow, guarantee that steam can follow electric fan heater 2 endogenous source constantly carry to thermal-insulated incubator 3 inside.
Further, the inside gas generator that can produce inert gas that is equipped with of electric fan heater 2, and its inside still is equipped with the heating element that can heat inert gas, heats inert gas through the heating element and makes it form steam, and the rethread fan carries to the inside of thermal-insulated incubator 3, satisfies the growth demand of its inside anaerobe species.
In this embodiment, the rear end of the heat insulation incubator 3 is composed of an inlet rear plate 36, two sides of the inlet rear plate 36 are provided with a first side plate 31 and a second side plate 33 which are parallel to each other, the upper and lower ends of the inlet rear plate 36, the first side plate 31 and the second side plate 33 are respectively provided with a top plate 32 and a heating bottom plate 35, the top plate 32 is fixedly connected with the first side plate 31 and the second side plate 33 respectively, the front sides of the heating bottom plate 35, the first side plate 31, the second side plate 33 and the top plate 32 are provided with a door 34, and the door 34 is rotatably arranged at the front end of the first side plate 31.
In this embodiment, the inlet rear plate 36 includes a first slide plate 361 and a second slide plate 362 that are parallel to each other, an intake valve 367 is disposed on the first slide plate 361 and the second slide plate 362, a third slide plate 363 is mounted in the intake valve 367 in a left-right moving manner, the third slide plate 363 is mounted between the first slide plate 361 and the second slide plate 362 in a left-right sealing manner, the first slide plate 361 and the second slide plate 362 are respectively and fixedly connected with the first side plate 31, the second side plate 33 and the top plate 32 in a sealing manner, a sliding groove 366 that is mutually communicated with the intake valve 367 is disposed on the second slide plate 362, a fixing block 364 is mounted in the sliding groove 366 in a left-right sliding manner, a fixing block 364 is mounted on one side of the third slide plate 363, and a threaded hole 365 is disposed in the fixing block 364.
In this embodiment, the heating bottom plate 35 is formed by a rectangular plate 351, the rectangular plate 351 is fixedly mounted on a side plate 31, a side plate 33, a slide plate 361 and a slide plate 362 respectively, a cavity 352 is arranged in the rectangular plate 351, a main pipe 354 is mounted in the cavity 352, a plurality of secondary pipes 353 which are mutually communicated are arranged on the main pipe 354, and a plurality of air outlet pipes 8 which are annularly and equidistantly arranged are mounted on each secondary pipe 353;
the rotary mechanism 9 is installed to the top of rectangular plate 351, and a plurality of bracing piece is installed to the below of rotary mechanism 9, and the top of rotary mechanism 9 is equipped with ring 357, and the below fixed mounting of ring 357 has asbestos net 358, every the top of outlet duct 8 all stretches into the below of asbestos net 358, is equipped with culture dish 38 on the asbestos net 358, and in the ring 357 was located to the end of culture dish 38, sealed fixed card was equipped with glass lid 39 on the culture dish 38 that anaerobic bacterial was used, for installing glass lid 39 on the culture dish 38 that aerobic bacterial was used, curb plate two 33 inboard was equipped with draw-in groove 37.
It should be added that the glass cover 39 is a closed cover body, and can be completely closed on the culture dish 38, and a sealing ring is arranged on the inner wall of the glass cover 39, so that the tightness of the culture dish 38 is ensured.
In this embodiment, the chamber door 34 is formed by a front plate 341, the front plate 341 is rotatably mounted on the front side of the first side plate 31, a heat dissipation hole 342 and a mark 343 are provided at the upper end of the front plate 341, a plurality of marks 343 are marked with a number of one to one hundred in sequence according to the arrangement sequence, labels corresponding to the numbers of the marks 343 are fixedly attached to the outer surfaces of the plurality of dishes 38, a glass observation window 345 is provided below the front plate 341, so that a user can observe the strain in the dishes 38 conveniently, a first circular rod 346 is rotatably mounted on one side of the front plate 341, two ends of the first circular rod 346 are respectively positioned on two sides of the front plate 341, the first circular rod 346 and the second side plate 33 are in contact with each other, a rectangular turn button 344 is fixedly mounted at the outer end of the first circular rod 346, a rectangular rod 347 is fixedly mounted at the inner end of the first circular rod 346, the turn button 344 drives the first circular rod 346 and the rectangular rod 347 to rotate, the rectangular rod 347 is used for turning the rectangular rod 347 into the clamping groove 37, and the front plate 341 cannot be opened after the rectangular rod 347 is arranged in the clamping groove 37.
It should be added that, the periphery of the asbestos screen 358 is also provided with a wrapping cover 6 capable of wrapping the asbestos screen therein, a cavity communicated with the cavity 352 is arranged in the wrapping cover 6, a plurality of ventilation holes 61 are formed in the side surface of the wrapping cover 6, and heat can enter the cavity in the wrapping cover 6 from the cavity 352, so that the heat escapes from the ventilation holes 61, and the periphery of the side surface of the culture dish 38 can be heated;
further, the rotating mechanism 9 is composed of a bottom supporting ring 91 and a rotating ring 93, the bottom supporting ring 91 is fixed on the supporting rod, an annular groove 92 is formed in the surface of the bottom supporting ring 91, a gear 96 is arranged on one side of the annular groove 92, the gear 96 stretches into the annular groove 92, the gear 96 is driven by a second motor 95 fixed on a rectangular plate 351, and a circle of convex teeth 94 is fixed on the outer ring surface of the rotating ring 93; the convex teeth 94 and the gear 96 are meshed with each other, the rotating ring 93 is rotatably arranged in the annular groove 92, and the top end of the rotating ring 93 is fixed with the upper annular ring 357;
it should be further added that the balls contacting with the rotating ring 93 are arranged on the groove wall of the annular groove 92, so that smoothness of the rotating process of the rotating ring 93 is ensured, the rotating ring 93 is driven to rotate by driving the gear 96 to rotate through the second motor 95, and accordingly the asbestos net 358 and the culture dish 38 thereon are driven to rotate, and uniformity of temperature of the culture dish 38 can be further improved in the rotating process.
It is further supplemented that ten braking screws 4 are installed on the heat insulation outer frame 1, the braking screws 4 are composed of first motors 41, the first motors 41 are fixedly installed on the heat insulation outer frame 1, the output ends of the first motors 41 are fixedly installed with second round rods 42 through couplings, the second round rods 42 are installed in threaded holes 365 in a threaded mode, one second round rod 42 penetrates through ten threaded holes 365 in each row of heat insulation incubator 3, ten external threads 43 are arranged on the second round rods 42, the external threads 43 are meshed with internal threads in the threaded holes 365, storage batteries are fixedly installed on the heat insulation outer frame 1, and the storage batteries are connected with the fan heater 2, the ten first motors 41 and a plurality of secondary pipelines 353 through power lines respectively.
It should be noted that, the first side plate 31, the top plate 32, the second side plate 33, the rectangular plate 351, the first slide 361, the second slide 362 and the third slide 363 used for forming the heat insulation incubator 3 are all made of heat insulation materials, the external screw thread 43 on the second circular rod 42 can be driven to rotate in a screw hole 365 in a positive and negative manner through the output end of the first motor 41, so that the third slide 363 is driven to be far away from or cover and seal the air inlet valve 367, when the third slide 363 covers and seals the air inlet valve 367, hot air used for isolating two groups of heat insulation incubators 3 enters the heat insulation incubator 3, when the third slide 363 is far away from the air inlet valve 367, the hot air between the two groups of heat insulation incubators 3 can enter the heat insulation incubator 3 through the air inlet valve 367, heat is used for heating the strains in the culture dish 38 in the heat insulation incubator 3, then enters each secondary pipeline 353 through the main pipeline 354, and then enters the upper part of the asbestos net from each air outlet pipe 8, thereby achieving the purpose of changing the temperature in the heat insulation incubator 3, the asbestos net 358 can prevent the ascending hot air from being directly transferred to the culture dish 38, the strains in the culture dish 38, and the culture dish 38 need to be evenly distributed by the asbestos net 358.
It should be noted that the intake valve 367 is connected to the cavity 352 through the connecting cover 7, and the hot air enters the connecting cover 7 through the intake valve 367 and then enters the cavity 352.
Further, the side plate I31 and the side plate II 33 which are in contact with the rectangular inner cavity are in sealing connection, the top plate 32 which is in contact with the heat insulation outer frame 1 is in sealing connection with the rectangular plate 351, the side plate I31 and the side plate II 33 which are in contact with each other are in sealing connection, and the top plate 32 which is in contact with each other is in sealing connection with the rectangular plate 351.
The method for culturing the target microorganism for treating the culture tail water comprises the following steps:
step one, confirming a target strain;
and step two, screening culture conditions.
The specific operation of the first step is as follows: firstly, extracting culture tail water as a microorganism detection sample, detecting the types and the relative abundance of microorganisms in the sample by a second-generation and third-generation high-throughput sequencing technology, and determining whether target microorganisms degrading and converting organic pollutants in the tail water exist in the sample.
The specific operation of the second step is as follows: the sample containing target microorganism is divided into a plurality of groups, the sample is used as cultured strains, the sample can be divided into a hundred groups at most, the culture plates are respectively placed in different culture dishes 38 for culturing, meanwhile, the temperature needs to be raised by a warm air blower 2, the space between the two groups of heat insulation culture boxes 3 is filled with hot air flow, the strain needing anaerobic comparison is sealed in the culture dishes 38 where the strain is located, a glass cover 39 is covered, then the sample is placed in the corresponding heat insulation culture boxes 3 according to the label on the culture dishes 38, a rectangular turn button 344 is turned, a rectangular rod 347 is driven to turn out of a clamping groove 37 through a first round rod 346, a box door 34 can be pulled, the culture dishes 38 are placed in an upper round ring 357 on an asbestos net 358, then the box door 34 is closed at the front side of the heat insulation culture boxes 3, the rectangular turn button 344 is turned, the rectangular rod 347 is driven to turn into the clamping groove 37 through a first round rod 346, the box door 34 can not be opened, the culture dish 38 for oxygen bacteria does not need to be covered with a glass cover 39, when the temperature of the culture dish 38 needs to be changed, the output end of the first motor 41 drives the external thread 43 on the second circular rod 42 to spirally rotate in the threaded hole 365, so as to drive the three slide 363 to be far away from the air inlet valve 367, hot air between the two groups of heat insulation culture boxes 3 is isolated and enters the heat insulation culture box 3, the temperature of the bacteria in the culture dish 38 in the heat insulation culture box 3 is increased, the purpose of changing the temperature in the heat insulation culture box 3 is achieved, when the temperature needed in the heat insulation culture box 3 is higher than the culture temperature needed by the bacteria, the three slide 363 covers and seals the air inlet valve 367, the asbestos net 358 can prevent rising hot air from being directly transmitted to the culture dish 38, the bacteria in the culture dish 38 is scalded, the culture dish 38 needs to uniformly distribute the hot air through the asbestos net 358, and then culturing a plurality of groups of strains in different temperature environments, detecting the strains in a plurality of groups of culture dishes 38 by a second-generation and third-generation high-throughput sequencing technology, sequentially detecting the number of strains in the groups of culture dishes 38, wherein the culture condition of the strain with the largest number is the culture condition required by the target strain, dividing the obtained target strain into one hundred groups, placing the hundred groups of target strains in a plurality of culture dishes 38, placing the culture dishes in a plurality of heat-insulating culture boxes 3, and culturing the culture dishes in the same environment.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The cultivation tail water treatment is with target microorganism culture apparatus includes:
the heat insulation outer frame (1) is internally provided with a rectangular inner cavity which is communicated with the heat insulation outer frame (1);
a warm air blower (2) arranged at the top end of the heat insulation outer frame (1);
the device is characterized by further comprising a plurality of heat insulation incubator (3) which are respectively arranged at the front side and the rear side of the rectangular inner cavity in a matrix manner and can receive hot air produced by the warm air blower (2), wherein the heat insulation incubator (3) comprises a culture dish (38) for culturing strains, the rear end of the heat insulation incubator (3) is composed of an inlet rear plate (36), the inlet rear plate (36) comprises an inlet valve (367), a sliding plate three (363) is arranged in the inlet valve (367) in a left-right moving manner, one side of the sliding plate three (363) is provided with a fixed block (364), and a threaded hole (365) is formed in the fixed block (364);
a plurality of braking screw (4), install on thermal-insulated frame (1), braking screw (4) are by first motor (41) constitution, second circular pole (42) are installed through the shaft coupling to the output of first motor (41), second circular pole (42) install in screw hole (365), just be equipped with a plurality of external screw thread (43) on second circular pole (42), external screw thread (43) with the internal thread intermeshing in screw hole (365).
2. The cultivation apparatus for targeted microorganism for treatment of tail water of cultivation according to claim 1, wherein: the two sides of the inlet rear plate (36) are provided with a first side plate (31) and a second side plate (33) which are parallel to each other, the upper end and the lower end of the inlet rear plate (36), the first side plate (31) and the second side plate (33) are respectively provided with a top plate (32) and a heating bottom plate (35), and the heating bottom plate (35), the first side plate (31), the second side plate (33) and the front side of the top plate (32) are provided with a box door (34).
3. The targeted microorganism culture apparatus for the treatment of culture tail water according to claim 2, wherein: the inlet rear plate (36) further comprises a first sliding plate (361) and a second sliding plate (362) which are parallel to each other, the third sliding plate (363) is slidably installed between the first sliding plate (361) and the second sliding plate (362), the first sliding plate (361) and the second sliding plate (362) are respectively connected with the first side plate (31), the second side plate (33) and the top plate (32) mutually, and the air inlet valve (367) is arranged on the first sliding plate (361) and the second sliding plate (362).
4. A targeted microorganism culture apparatus for treatment of aquaculture tail water according to claim 3, wherein: and a sliding groove (366) communicated with the air inlet valve (367) is formed in the second sliding plate (362), and the fixed block (364) is installed in the sliding groove (366).
5. The targeted microorganism culture apparatus for the treatment of culture tail water according to claim 4, wherein: the heating bottom plate (35) comprises a rectangular plate (351), the rectangular plate (351) respectively with curb plate one (31) curb plate two (33) slide one (361) slide two (362) interconnect, be equipped with cavity (352) in the rectangular plate (351), install trunk line (354) in cavity (352), be equipped with a plurality of follow pipeline (353) of intercommunication on trunk line (354), every all install a plurality of outlet duct (8) that are annular equidistant range from pipeline (353), rotary mechanism (9) are installed to the top of rectangular plate (351), a plurality of bracing piece is installed to the below of rotary mechanism (9), the top of rotary mechanism (9) is equipped with ring (357), the below of going up ring (357) is installed asbestos net (358), every the top of outlet duct (8) all stretches into the below of asbestos net (358).
6. The cultivation apparatus for targeted microorganism for tail water treatment according to claim 5, wherein: the culture dish (38) is arranged on the asbestos screen (358), the bottom end of the culture dish (38) is arranged in the upper circular ring (357), a glass cover (39) is arranged on the culture dish (38) for anaerobic bacteria, and a clamping groove (37) is formed in the inner side of the second side plate (33).
7. The targeted microorganism culture apparatus for the treatment of culture tail water according to claim 2, wherein: the refrigerator door (34) is composed of a front plate (341), the front plate (341) is rotatably mounted on the front side of the side plate I (31), a heat dissipation hole (342) and a mark (343) are formed in the upper end of the front plate (341), and a glass observation window (345) is formed below the front plate (341).
8. A method for culturing a target microorganism for treating a culture tail water, using the target microorganism culture apparatus for treating a culture tail water according to any one of claims 1 to 7, characterized in that: the method comprises the following steps:
step one, confirming a target strain;
and step two, screening culture conditions.
9. The method for culturing a target microorganism for treating a tailwater for cultivation according to claim 8, wherein: the specific operation of the first step is as follows: firstly, extracting culture tail water as a microorganism detection sample, then detecting the types and the relative abundance of microorganisms in the sample by a second-generation and third-generation high-throughput sequencing technology, and determining whether target microorganisms exist in the sample.
10. The method for culturing a target microorganism for treating a tailwater for cultivation according to claim 9, wherein: the specific operation of the second step is as follows: step one, confirming a sample containing target microorganisms, respectively culturing, separating, purifying and the like through culture dishes (38) with anaerobic or aerobic culture, different formulas and temperatures, finding target strains, carrying out high-throughput sequencing identification on the purified target strains, and determining the target strains from the gene level.
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| CN202311035946.7A CN116751654B (en) | 2023-08-17 | 2023-08-17 | Targeted microorganism culture equipment for culture tail water treatment and culture method thereof |
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| GB926541A (en) * | 1958-06-05 | 1963-05-22 | Mini Agriculture & Fisheries | Improvements in or relating to the cultivation and sterilization of bacteria and the like |
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| GB926541A (en) * | 1958-06-05 | 1963-05-22 | Mini Agriculture & Fisheries | Improvements in or relating to the cultivation and sterilization of bacteria and the like |
| CN110951589A (en) * | 2020-01-03 | 2020-04-03 | 江秀燕 | Microbial detection clean culture device and use method thereof |
| CN112143638A (en) * | 2020-10-10 | 2020-12-29 | 佳木斯大学 | Temperature-adjustable microbial culture equipment and method |
| CN115287173A (en) * | 2022-08-12 | 2022-11-04 | 淮安市第二人民医院 | Storehouse is cultivateed to multi-functional clinical laboratory microorganism bacterium dual temperature |
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