CN116748279A - Ultrasonic enzymolysis recycling method and system for livestock dying of illness - Google Patents
Ultrasonic enzymolysis recycling method and system for livestock dying of illness Download PDFInfo
- Publication number
- CN116748279A CN116748279A CN202310992785.4A CN202310992785A CN116748279A CN 116748279 A CN116748279 A CN 116748279A CN 202310992785 A CN202310992785 A CN 202310992785A CN 116748279 A CN116748279 A CN 116748279A
- Authority
- CN
- China
- Prior art keywords
- ultrasonic
- tank
- enzymolysis
- phase material
- livestock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000144972 livestock Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000004064 recycling Methods 0.000 title claims abstract description 32
- 239000000463 material Substances 0.000 claims abstract description 86
- 238000002156 mixing Methods 0.000 claims abstract description 61
- 238000000227 grinding Methods 0.000 claims abstract description 27
- 239000012071 phase Substances 0.000 claims abstract description 26
- 239000000919 ceramic Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000001914 filtration Methods 0.000 claims abstract description 22
- 230000007062 hydrolysis Effects 0.000 claims abstract description 21
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 21
- 235000013372 meat Nutrition 0.000 claims abstract description 21
- 239000007790 solid phase Substances 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 14
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- 239000002002 slurry Substances 0.000 claims abstract description 11
- 238000003825 pressing Methods 0.000 claims abstract description 8
- 239000002131 composite material Substances 0.000 claims abstract description 4
- 238000007599 discharging Methods 0.000 claims description 45
- 239000000706 filtrate Substances 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 17
- 239000000047 product Substances 0.000 claims description 14
- 238000003860 storage Methods 0.000 claims description 14
- 239000007791 liquid phase Substances 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 9
- 238000012546 transfer Methods 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 8
- 239000004519 grease Substances 0.000 claims description 7
- 108091005508 Acid proteases Proteins 0.000 claims description 4
- 108090000145 Bacillolysin Proteins 0.000 claims description 4
- 108091005658 Basic proteases Proteins 0.000 claims description 4
- 108010004032 Bromelains Proteins 0.000 claims description 4
- 239000004367 Lipase Substances 0.000 claims description 4
- 102000004882 Lipase Human genes 0.000 claims description 4
- 108090001060 Lipase Proteins 0.000 claims description 4
- 102000035092 Neutral proteases Human genes 0.000 claims description 4
- 108091005507 Neutral proteases Proteins 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 235000019835 bromelain Nutrition 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 235000019421 lipase Nutrition 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 235000019362 perlite Nutrition 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 14
- 238000009826 distribution Methods 0.000 abstract description 8
- 230000000052 comparative effect Effects 0.000 description 41
- 239000011159 matrix material Substances 0.000 description 15
- 239000000523 sample Substances 0.000 description 15
- 230000005855 radiation Effects 0.000 description 8
- 244000144977 poultry Species 0.000 description 7
- 235000013594 poultry meat Nutrition 0.000 description 7
- 230000000191 radiation effect Effects 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000012423 maintenance Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000272517 Anseriformes Species 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/30—Destroying solid waste or transforming solid waste into something useful or harmless involving mechanical treatment
- B09B3/35—Shredding, crushing or cutting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/30—Destroying solid waste or transforming solid waste into something useful or harmless involving mechanical treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/60—Biochemical treatment, e.g. by using enzymes
- B09B3/65—Anaerobic treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/70—Chemical treatment, e.g. pH adjustment or oxidation
Landscapes
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses an ultrasonic enzymolysis recycling method and system for livestock dying of illness, and relates to the technical field of recycling treatment for livestock dying of illness. The method comprises the following steps: (1) grinding; (2) mincing meat; (3) mixing; (4) hydrolysis; (5) horizontal centrifugation; (6) butterfly centrifugation; (7) ultrasonic enzymolysis: adding 2.5-5%wt of composite biological enzyme into the solid phase material obtained in the step (5) and the water phase material obtained in the step (6) for ultrasonic enzymolysis, and stirring for 2-3 hours at 55-62 ℃ to obtain an enzymolysis material; (8) filtering and mixing; (9) plate-frame filter pressing; (10) ceramic filtration; and (11) blending. According to the invention, the livestock dying of illness are subjected to resource treatment in an ultrasonic mode, the uniformity of material slurry is changed by utilizing ultrasonic waves, the treatment speed and the treatment effect are accelerated, the more uniform protein peptide molecular distribution is obtained, and the influence of other organic molecules on the enzymolysis process is reduced.
Description
Technical Field
The invention relates to the technical field of livestock resource treatment, in particular to an ultrasonic enzymolysis resource treatment method and system for livestock dead of diseases.
Background
The dead animals not only bring hidden danger to the ecological environment and public health safety, but also bring huge economic loss to the production of animal husbandry. Taking pig raising as an example, the feed cost is about 70% of the total feeding cost, while the raw materials such as bean pulp, corn and the like required by the feed are largely imported, so that the animals died from illness are waste of national resources. Therefore, the exploration of the recycling technology of the treatment byproducts has great significance, and the existing recycling technology of the animals dying of diseases, such as wet method treatment, has the technical problems of oil-water separation and sewage treatment of the products; the product treated by the high-temperature fermentation method has the problem of higher grease content when used as an organic fertilizer. Thus, there is a need to find a method for recycling livestock dying of illness.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for recycling livestock dying of illness through ultrasonic enzymolysis and a system thereof, which concretely comprises the following technical scheme:
in a first aspect, a method for recycling livestock dying of illness through ultrasonic enzymolysis is provided, which comprises the following steps:
(1) Grinding; grinding the livestock dead of the disease;
(2) Mincing meat; mincing the crushed dead livestock;
(3) Mixing: the mass ratio of the livestock dying of illness to the clean water after meat mincing treatment is 1: (1-4) mixing to form a slurry of the material;
(4) Hydrolysis: continuously stirring the material slurry for 1-2 hours at the temperature of 100-105 ℃ to complete hydrolysis;
(5) Horizontal centrifugation: carrying out solid-liquid separation on the hydrolyzed material slurry to obtain a solid-phase material and a liquid-phase material;
(6) Butterfly centrifugation: oil-water separation is carried out on the liquid phase material through butterfly centrifugation, so that a water phase material and an oil phase material are obtained, and the oil phase material is stored;
(7) Ultrasonic enzymolysis: adding 2.5-5%wt of composite biological enzyme into the solid phase material obtained in the step (5) and the water phase material obtained in the step (6) for ultrasonic enzymolysis, and stirring for 2-3 hours at 55-62 ℃ to obtain an enzymolysis material;
(8) Filtration-assisting mixing: mixing the filter aid and the enzymolysis material according to the mass ratio of 0.5-2.0%, and then completing filtration-assisting mixing treatment;
(9) Press filtration of the plate and the frame: carrying out plate-frame filter pressing on the materials subjected to the auxiliary filtering mixed treatment, wherein the density of filter cloth for the plate-frame filter pressing is 600-800 meshes, and filtering materials and filtrate are obtained;
(10) And (3) ceramic filtration: filtering the filtrate with the filtering precision of 100-120nm to obtain protein peptide liquid;
(11) And (3) blending: and (3) regulating the concentration and the pH value of the protein peptide liquid to obtain a prepared product when the content of free amino acid is 200-300g/L and the pH value is 4-7.5.
Further, in the step (7), the ultrasonic power of the ultrasonic enzymolysis is 0.3-0.5w/cm 2 The frequency is 20-25kHz.
In step (7), the complex biological enzyme is a mixture of papain, alkaline protease, neutral protease, acid protease, bromelain and lipase.
Preferably, in the step (7), the compound biological enzyme consists of 15 parts of papain, 20 parts of alkaline protease, 7 parts of neutral protease, 5 parts of acid protease, 1 part of bromelain and 3 parts of lipase in parts by mass.
Further, in the step (8), the filter aid is a mixture of diatomite and perlite.
Preferably, in the step (8), the filter aid consists of 1 part by mass of diatomite and 1 part by mass of perlite.
Further, the step (9) and the step (10) also comprise the step of temporarily storing the filtrate for 0.5-1h.
Further, the step (11) further comprises the step of keeping the prepared product in a dark place and sealing.
Further, in the step (1), the grain size of the dead livestock after the crushing treatment is 5-8cm.
Further, in the step (2), the grain size of the livestock dying of illness after meat mincing treatment is 4-8mm.
Preferably, the grinding treatment of the step (1) is carried out in a grinding mill, the meat mincing treatment of the step (2) is carried out in a meat grinder, the mixing of the step (3) is carried out in a mixing tank, the hydrolysis of the step (4) is carried out in a hydrolysis tank, the solid-liquid separation of the step (5) is carried out in a horizontal centrifuge, the oil-water separation of the step (6) is carried out in a disk centrifuge, the oil tank material of the step (6) is stored in a grease transfer barrel, the ultrasonic enzymolysis of the step (7) is carried out in an ultrasonic enzymolysis tank, the filtration-assisting mixing treatment of the step (8) is carried out in a filtration-assisting mixing tank, the plate-and-frame filter pressing of the step (9) is carried out in a plate-and-frame filter press, the filtration of the step (10) is carried out in a ceramic filter, and the concentration and pH value adjustment of the step (11) are carried out in a blending tank.
Preferably, the temporary storage step between the step (9) and the step (10) is performed in a temporary storage tank.
Preferably, the steps of light shielding and sealed storage of step (11) are performed in a sealed can.
In a second aspect, a system for implementing the method for recycling ultrasonic enzymolysis of dead livestock according to the first aspect is provided, which comprises a grinding mill, a meat grinder, a mixing tank, a hydrolysis tank, a horizontal centrifuge, a disc centrifuge, a grease transfer barrel, an ultrasonic enzymolysis tank, a filtration-assisting mixing tank, a plate-and-frame filter press, a ceramic filter and a blending tank;
the discharging end of the grinding machine is connected with the meat grinder; the discharging end of the meat grinder is connected with the mixing tank; the discharging end of the mixing tank is connected with the hydrolysis tank; the discharging end of the hydrolysis tank is connected with a horizontal centrifuge; the discharging end of the horizontal centrifuge comprises a solid-phase material discharging end and a liquid-phase material discharging end; the solid-phase material discharging end is connected with an ultrasonic enzymolysis tank; the liquid phase material discharging end is connected with a disc type centrifuge; the discharge end of the disc centrifuge comprises a water phase material discharge end and an oil phase material discharge end; the oil phase material discharging end is connected with the oil transfer barrel; the water phase material discharging end is connected with an ultrasonic enzymolysis tank; the discharge end of the ultrasonic enzymolysis tank is connected with a filtering-assisting mixing tank; the discharging end of the auxiliary filtering mixing tank is connected with a plate-and-frame filter press; the discharging end of the plate-and-frame filter press comprises a filter material discharging end and a filtrate discharging end; the filtrate discharge end is connected with a ceramic filter; the discharge end of the ceramic filter is connected with the blending tank.
Further, the system for realizing the ultrasonic enzymolysis recycling method of the livestock dying of illness also comprises a temporary storage tank and a sealing tank; the temporary storage tank is arranged between the filtrate discharge end and the ceramic filter, and the sealing tank is connected with the discharge end of the blending tank.
Preferably, the grinding machine is used for grinding the dead livestock, and the grinding machine is used for crushing and grinding the dead livestock through double-wheel tooth biting.
Preferably, a booster pump is further arranged between the temporary storage tank and the ceramic filter.
Preferably, a conveyor belt is arranged between the solid-phase material discharging end and the ultrasonic enzymolysis tank.
Preferably, the filter element of the ceramic filter is a ceramic filter element.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the livestock dying of illness are subjected to recycling treatment in an ultrasonic mode, the uniformity of material slurry is changed by utilizing ultrasonic waves, and the distribution mode of protein molecules and biological enzyme molecules in the feed liquid is changed from a molecular level, so that the enzymolysis efficiency and effect are promoted, the treatment speed and treatment effect are accelerated, more uniform protein peptide molecular distribution is obtained, and the influence of other organic molecules on the enzymolysis process is reduced.
The ultrasonic enzymolysis recycling method and system for the dead poultry and livestock can rapidly realize complete recycling treatment of the dead poultry and livestock carcasses, extract valuable substances in the dead poultry and livestock carcasses, and prepare reusable product raw materials, wherein the biological oil is used for processing biomass diesel, and complex nutrients such as protein nutrition, polysaccharide substances and the like can be used in the fields of agricultural production and planting industry and the like after being extracted by small molecules.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic flow chart of an ultrasonic enzymolysis recycling method for livestock dying of illness according to an embodiment of the invention;
FIG. 2 is a schematic flow chart of a system for implementing a method for ultrasonic enzymolysis recycling of livestock dying of illness according to an embodiment of the invention;
FIG. 3 is a schematic side view of an ultrasonic enzymolysis tank according to an embodiment of the invention;
FIG. 4 is a schematic top view of an ultrasonic enzymolysis tank according to an embodiment of the invention;
FIG. 5 is a schematic diagram showing the front view of an ultrasonic generator according to an embodiment of the present invention;
FIG. 6 is a schematic side view of an ultrasonic generator according to an embodiment of the present invention;
FIG. 7 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to the embodiment of the invention;
FIG. 8 is a top view of an ultrasonic interference coverage area of an ultrasonic enzymolysis tank according to an embodiment of the invention;
FIG. 9 is a side view of ultrasonic energy radiation effect of an ultrasonic enzymolysis tank according to an embodiment of the invention;
FIG. 10 is a side view of an ultrasonic interference coverage area diagram of an ultrasonic enzymolysis tank according to an embodiment of the invention;
FIG. 11 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to comparative example 1 of the present invention;
FIG. 12 is a top view of an ultrasonic interference coverage area of an ultrasonic enzymolysis tank according to comparative example 1 of the invention;
FIG. 13 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to comparative example 2 of the invention;
FIG. 14 is a top view of an ultrasonic interference coverage area of an ultrasonic enzymolysis tank according to comparative example 2 of the invention;
FIG. 15 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to comparative example 3 of the present invention;
FIG. 16 is a top view of the ultrasonic interference coverage area of the ultrasonic enzymolysis tank of comparative example 3 of the invention;
FIG. 17 is a side view of ultrasonic energy radiation effect of the ultrasonic enzymolysis tank of comparative example 4 of the present invention;
FIG. 18 is a side view of an ultrasonic interference coverage area of an ultrasonic enzymolysis tank according to comparative example 4 of the invention;
FIG. 19 is a side view of ultrasonic energy radiation effect of the ultrasonic enzymolysis tank of comparative example 5 of the present invention;
FIG. 20 is a side view of an ultrasonic interference coverage area diagram of an ultrasonic enzymolysis tank according to comparative example 5 of the invention;
FIG. 21 is a graph showing the effect of side view ultrasonic energy irradiation of the ultrasonic enzymolysis tank of comparative example 6 of the present invention;
FIG. 22 is a side view of the ultrasonic interference coverage of the ultrasonic enzymolysis tank of comparative example 6 of the invention.
The figure identifies the description:
1-a grinding roll; 2-meat grinder; 3-a mixing tank; 4-a hydrolysis tank; 5-a horizontal centrifuge; 6-disc centrifuge; 7-a conveyor belt; 8-an oil transfer barrel; 9-an ultrasonic enzymolysis tank; 10-a filtration-assisting mixing tank; 11-plate and frame filter press; 12-a booster pump; 13-a temporary storage tank; 14-a ceramic filter; 15-a blending tank; 16-sealing the tank; 91-an inner tank; 92-an ultrasonic wave generating matrix; 93-an ultrasonic generator; 94-a waterproof housing; 95-an ultrasonic wave generating probe; 96-stirring paddles; 97-differential motor; 98-spoiler fins; 99-a discharge hole; 910-an insulating layer; 911-temperature control jacket; 912-liquid level probe; 913—a temperature probe; 914-acid-base probe; 915-maintenance port.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention.
The terms "first," "second," "third," and the like are used merely to distinguish between descriptions and are not to be construed as indicating or implying relative importance.
The livestock and poultry died of illness in the invention include but are not limited to one of pigs died of illness, chickens died of illness, ducks died of illness, geese died of illness, pigeons died of illness, cattle died of illness or sheep died of illness, and the invention is not limited.
Example 1
The ultrasonic enzymolysis recycling method for the livestock dying of illness shown in figure 1 comprises the following steps:
(1) Grinding; taking the dead livestock as a raw material to carry out grinding treatment in a grinding machine, wherein the grain size of the dead livestock after the grinding treatment is 6cm;
(2) Mincing meat; grinding the crushed dead livestock in a meat grinder, wherein the grain size of the ground dead livestock is 6mm;
(3) Mixing: the mass ratio of the livestock dying of illness to the clean water after meat mincing treatment is 1:1, mixing in a mixing tank to form material slurry;
(4) Hydrolysis: continuously stirring the material slurry in a hydrolysis tank for 1.5 hours at the temperature of 100 ℃ to complete hydrolysis; continuous stirring can prevent solid blockage;
(5) Horizontal centrifugation: carrying out solid-liquid separation on the hydrolyzed material slurry in a horizontal centrifuge to obtain a solid-phase material and a liquid-phase material;
(6) Butterfly centrifugation: carrying out oil-water separation on the liquid phase material in a disc centrifuge through butterfly centrifugation to obtain a water phase material and an oil phase material, and placing the oil phase material in a grease transfer barrel for storage;
(7) Ultrasonic enzymolysis: adding 3%wt of composite biological enzyme into the solid phase material obtained in the step (5) and the water phase material obtained in the step (6) to carry out ultrasonic enzymolysis in an ultrasonic enzymolysis tank, and stirring for 2 hours at 60 ℃ to obtain a material after enzymolysis; the ultrasonic power of the ultrasonic enzymolysis is 0.3w/cm 2 The frequency is 25kHz; the compound biological enzyme consists of 15 parts of papain, 20 parts of alkaline protease, 7 parts of neutral protease, 5 parts of acid protease, 1 part of bromelain and 3 parts of lipase by mass;
(8) Filtration-assisting mixing: mixing the filter aid and the enzymolysis materials in a filtering-assisting mixing tank according to the mass ratio of 1%, and then completing filtering-assisting mixing treatment; the filter aid consists of 1 part of diatomite and 1 part of perlite in parts by mass;
(9) Press filtration of the plate and the frame: carrying out plate-and-frame filter pressing on the material subjected to the filtering-assisting mixed treatment in a plate-and-frame filter press, wherein the density of filter cloth of the plate-and-frame filter pressing is 700 meshes, so as to obtain filter materials and filtrate;
(10) And (3) ceramic filtration: filtering the filtrate in a ceramic filter with the filtering precision of 100nm to obtain protein peptide liquid;
the step (9) and the step (10) also comprise the step of temporarily storing the filtrate in a temporary storage tank, wherein the temporary storage time is 1h.
(11) And (3) blending: regulating the concentration and the pH value of the protein peptide liquid in a blending tank to obtain a blended product when the content of free amino acid is 250g/L and the pH value is 6; and (5) carrying out light-shielding and sealing preservation on the prepared product in a sealed tank.
The system for realizing the ultrasonic enzymolysis recycling method of the livestock dying of illness as shown in fig. 2 comprises a grinding machine 1, a meat grinder 2, a mixing tank 3, a hydrolysis tank 4, a horizontal centrifuge 5, a disc centrifuge 6, a grease transfer barrel 8, an ultrasonic enzymolysis tank 9, a filtration-assisting mixing tank 10, a plate-and-frame filter press 11, a ceramic filter 14 and a blending tank 15;
the discharging end of the grinding machine 1 is connected with a meat grinder 2; the discharge end of the meat grinder 2 is connected with a mixing tank 3. The grinding machine 1 is used for grinding the dead livestock, and the grinding machine 1 is used for crushing and grinding the dead livestock through double-wheel tooth biting.
The discharging end of the mixing tank 3 is connected with a hydrolysis tank 4; the discharging end of the hydrolysis tank 4 is connected with a horizontal centrifuge 5; the discharging end of the horizontal centrifuge 5 comprises a solid-phase material discharging end and a liquid-phase material discharging end; the solid material discharge end is connected with an ultrasonic enzymolysis tank 9; and a conveyor belt 7 is arranged between the solid-phase material discharge end and the ultrasonic enzymolysis tank 9. The liquid phase material discharging end is connected with a disc type centrifuge 6; the discharge end of the disc centrifuge 6 comprises a water phase material discharge end and an oil phase material discharge end; the oil phase material discharging end is connected with the grease transfer barrel 8; the water phase material discharge end is connected with an ultrasonic enzymolysis tank 9;
the discharge end of the ultrasonic enzymolysis tank 9 is connected with a filtration-assisting mixing tank 10; the discharging end of the auxiliary filtering mixing tank 10 is connected with a plate-and-frame filter press 11; the discharging end of the plate-and-frame filter press 11 comprises a filter material discharging end and a filtrate discharging end; the filtrate discharge end is connected with a ceramic filter 14; the filter liquor discharging end and the ceramic filter 14 are connected through a temporary storage tank 13, and a booster pump 12 is arranged between the temporary storage tank 13 and the ceramic filter 14. The filter element of the ceramic filter 14 is a ceramic filter element. The discharge end of the ceramic filter 14 is connected with a blending tank 15. The discharging end of the blending tank 15 is connected with a sealing tank 16.
In this embodiment, the apparatus further includes a plurality of pH adjuster dosing apparatuses, which are respectively connected to the mixing tank 3, the hydrolysis tank 4, and the blending tank 15.
FIG. 3 is a schematic side view of an ultrasonic enzymolysis tank according to an embodiment of the invention; FIG. 4 is a schematic top view of an ultrasonic enzymolysis tank according to an embodiment of the invention; FIG. 5 is a schematic diagram showing the front view of an ultrasonic generator according to an embodiment of the present invention; FIG. 6 is a schematic side view of an ultrasonic generator according to an embodiment of the present invention; FIG. 7 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to the embodiment of the invention; FIG. 8 is a top view of an ultrasonic interference coverage area of an ultrasonic enzymolysis tank according to an embodiment of the invention; FIG. 9 is a side view of ultrasonic energy radiation effect of an ultrasonic enzymolysis tank according to an embodiment of the invention; fig. 10 is a side view of an ultrasonic interference coverage area diagram of an ultrasonic enzymolysis tank according to an embodiment of the invention.
The ultrasonic enzymolysis tank 9 comprises an inner tank 91 and an ultrasonic generation matrix 92, the ultrasonic generation matrix 92 is arranged on the middle part, the lower part and the bottom wall of the side wall of the inner tank 91, and the ultrasonic generation matrix 92 is composed of a plurality of ultrasonic generators 93 which are equidistantly arranged. In this embodiment, the ultrasonic generating matrix 92 is composed of 8 ultrasonic generators 93 arranged at equal intervals. The ultrasonic generators 93 are symmetrically arranged to form a regular octagonal ultrasonic generating matrix 92. The ultrasonic generator 93 is arranged in the waterproof housing 94, and a plurality of uniformly distributed ultrasonic generating probes 95 are arranged on the side wall of the ultrasonic generator 93. The ultrasonic generating probe 95 may emit ultrasonic energy.
The top of the inner tank 91 is provided with a stirring paddle 96, and the bottom of the stirring paddle 96 extends to the inside of the inner tank 91. The top of the stirring paddle 96 is connected with a differential motor 97. The ultrasonic enzymolysis tank 9 further comprises turbulence fins 98, and the turbulence fins 98 are fixedly arranged on two opposite sides of the lower part of the inner tank 91. The stirring paddle 96 can be driven to rotate by starting the differential motor 97. The bottom of the inner tank 91 is provided with a discharge outlet 99. The stirring paddle 96 in this embodiment can strengthen the protection of the discharge port 99, and avoid the blockage of the discharge port 99 by solid materials with high viscosity. And meanwhile, the turbulence fin plates 98 are combined, so that a liquid flow form of up-and-down turbulence can be formed in the inner tank body 91, and the liquid in the inner tank body 91 can be quickly and uniformly mixed.
The ultrasonic enzymolysis tank 9 further comprises an insulating layer 910 and a temperature control jacket 911, wherein the insulating layer 910 is arranged on the outer side of the inner tank 91, and the temperature control jacket 911 is arranged between the inner tank 91 and the insulating layer 910. One of steam, heat conducting oil and water can be introduced into the temperature control jacket 911. The steam is introduced into the temperature control jacket 911 in this embodiment to heat or cool the material in the inner tank 91, so as to achieve the purpose of controlling the temperature of the material. The ultrasonic enzymolysis tank 9 further comprises a liquid level probe 912, a temperature probe 913 and an acid-base probe 914, wherein the liquid level probe 912 is arranged on the middle part and the upper part of the side wall of the inner tank 91 and the bottom wall, and the temperature probe 913 and the acid-base probe 914 are both arranged on the side wall of the inner tank 91. The liquid level probe 912, the temperature probe 913 and the acid-base probe 914 are used for analyzing and regulating specific parameters in materials, thereby realizing the conditional controllable reaction of the biological enzyme reaction. The top of the inner tank 91 is provided with a maintenance port 915, the maintenance port 915 penetrates through the heat insulation layer 910 and the temperature control jacket 911, and the ultrasonic enzymolysis 9 tank can be overhauled through the maintenance port 915.
Comparative example 1
FIG. 11 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to comparative example 1 of the present invention; FIG. 12 is a top view of the ultrasonic interference coverage area of the ultrasonic enzymolysis tank of comparative example 1 of the invention. Comparative example 1 is different from example 1 in that the ultrasonic wave generation matrix is composed of 4 ultrasonic wave generators arranged at equal intervals, and the ultrasonic wave generation matrix is arranged only on the middle part of the side wall of the inner tank.
Comparative example 2
FIG. 13 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to comparative example 2 of the invention; FIG. 14 is a top view of the ultrasonic interference coverage area of the ultrasonic enzymolysis tank of comparative example 2 of the invention. Comparative example 2 is different from example 1 in that the ultrasonic wave generation matrix is composed of 3 ultrasonic wave generators arranged at equal intervals, and the ultrasonic wave generation matrix is arranged only on the middle portion of the side wall of the inner tank.
Comparative example 3
FIG. 15 is a graph showing the effect of ultrasonic energy radiation from a top view of an ultrasonic enzymolysis tank according to comparative example 3 of the present invention; FIG. 16 is a top view of the ultrasonic interference coverage of the ultrasonic enzymolysis tank of comparative example 3 of the invention. Comparative example 3 is different from example 1 in that the ultrasonic wave generation matrix is composed of 6 ultrasonic wave generators arranged at equal intervals, and the ultrasonic wave generation matrix is arranged only on the middle portion of the side wall of the inner tank.
Comparative example 4
FIG. 17 is a side view of ultrasonic energy radiation effect of the ultrasonic enzymolysis tank of comparative example 4 of the present invention; FIG. 18 is a side view of the ultrasonic interference coverage area of the ultrasonic enzymolysis tank of comparative example 4 of the invention. Comparative example 4 in comparison with example 1, the ultrasonic wave generating matrix was disposed on the middle and lower portions of the side wall of the inner tank.
Comparative example 5
FIG. 19 is a side view of ultrasonic energy radiation effect of the ultrasonic enzymolysis tank of comparative example 5 of the present invention; FIG. 20 is a side view of the ultrasonic interference coverage area of the ultrasonic enzymolysis tank of comparative example 5 of the invention. Comparative example 5 in comparison with example 1, the ultrasonic wave generating matrix was disposed on the middle, lower and bottom portions of the side wall of the inner tank.
Comparative example 6
FIG. 21 is a graph showing the effect of side view ultrasonic energy irradiation of the ultrasonic enzymolysis tank of comparative example 6 of the present invention; FIG. 22 is a side view of the ultrasonic interference coverage of the ultrasonic enzymolysis tank of comparative example 6 of the invention. Comparative example 6 in comparison with example 1, the ultrasonic wave generating matrix was provided on the lower portion of the side wall of the inner tank.
Comparative example 7
Comparative example 7 is different from example 1 in that ultrasonic enzymolysis is not performed in the step (7), but the solid phase material obtained in the step (5) and the water phase material obtained in the step (6) are added with 3%wt of compound biological enzyme, and then are subjected to enzymolysis, and are stirred for 2 hours at 60 ℃ to obtain the material after enzymolysis, and the rest conditions are the same.
The ultrasonic enzymolysis recycling method for dead livestock of example 1 and comparative examples 1-7 was subjected to enzymolysis test, and the average molecular weight (daltons) of the reaction product (the material after enzymolysis) and the molecular weight (daltons) distribution of the reaction product were respectively calculated based on the enzymolysis time of 2 hours, and the test results are shown in table 1 below:
TABLE 1 results of the ultrasonic enzymatic hydrolysis of dead birds and animals of example 1, comparative examples 1-7
As shown in the test results of the table 1, the uniformity of the reaction area in the tank is more and more stable along with the increase of the number of the ultrasonic generators, and the reaction efficiency is greatly improved within a certain reaction time; at the same time, we found that as the number increases, the control of the molecular weight distribution is more concentrated, which is related to the difference of the distribution areas of the molecules with different molecular weight distribution under different ultrasonic reaction conditions, resulting in the conditional change of the contact efficiency of the protein peptide molecules with different molecular weight and enzymes, thereby achieving the effect of adjusting the target object of the enzymolysis reaction through the change of the position of the ultrasonic generator.
Through repeated experiments, 8 ultrasonic generators are arranged on each layer of the side wall, and the scheme of covering the side wall and the bottom wall can achieve the scheme of optimal yield of protein peptide molecules in the interval of 460-780 daltons, wherein the interval protein peptide mainly consists of functional small peptides composed of 3-10 amino acids and is a main active substance distribution area.
As can be seen from the comparison of the method for recycling the enzymolysis of the dead livestock in example 1 and comparative example 7, the enzymolysis treatment is not performed in an ultrasonic manner, and the enzymolysis efficiency is low. The yield of the target product is 2-6 times higher than that of the target product which is not used in the enzymolysis treatment by adopting an ultrasonic mode.
The ultrasonic enzymolysis recycling method and system for the dead poultry and livestock can rapidly realize complete recycling treatment of the dead poultry and livestock carcasses, extract valuable substances in the dead poultry and livestock carcasses, and prepare reusable product raw materials, wherein the biological oil is used for processing the biomass diesel, and complex nutrition such as protein nutrition, polysaccharide substances and the like can be used in the fields of agricultural production and planting industry and the like after being extracted by small molecules.
In another embodiment, a sealed can may be used to can the formulated product into a ton can as desired.
While the invention has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions of equivalents may be made and equivalents will be apparent to those skilled in the art without departing from the scope of the invention. Therefore, the protection scope of the invention is subject to the protection scope of the claims.
Claims (10)
1. The ultrasonic enzymolysis recycling method for the livestock dying of illness is characterized by comprising the following steps:
(1) Grinding; grinding the livestock dead of the disease;
(2) Mincing meat; mincing the crushed dead livestock;
(3) Mixing: the mass ratio of the livestock dying of illness to the clean water after meat mincing treatment is 1: (1-4) mixing to form a slurry of the material;
(4) Hydrolysis: continuously stirring the material slurry for 1-2 hours at the temperature of 100-105 ℃ to complete hydrolysis;
(5) Horizontal centrifugation: carrying out solid-liquid separation on the hydrolyzed material slurry to obtain a solid-phase material and a liquid-phase material;
(6) Butterfly centrifugation: oil-water separation is carried out on the liquid phase material through butterfly centrifugation, so that a water phase material and an oil phase material are obtained, and the oil phase material is stored;
(7) Ultrasonic enzymolysis: adding 2.5-5%wt of composite biological enzyme into the solid phase material obtained in the step (5) and the water phase material obtained in the step (6) for ultrasonic enzymolysis, and stirring for 2-3 hours at 55-62 ℃ to obtain an enzymolysis material;
(8) Filtration-assisting mixing: mixing the filter aid and the enzymolysis material according to the mass ratio of 0.5-2.0%, and then completing filtration-assisting mixing treatment;
(9) Press filtration of the plate and the frame: carrying out plate-frame filter pressing on the materials subjected to the auxiliary filtering mixed treatment, wherein the density of filter cloth for the plate-frame filter pressing is 600-800 meshes, and filtering materials and filtrate are obtained;
(10) And (3) ceramic filtration: filtering the filtrate with the filtering precision of 100-120nm to obtain protein peptide liquid;
(11) And (3) blending: and (3) regulating the concentration and the pH value of the protein peptide liquid to obtain a prepared product when the content of free amino acid is 200-300g/L and the pH value is 4-7.5.
2. The method for recycling ultrasonic enzymolysis of livestock dying of illness according to claim 1, wherein in step (7), the ultrasonic power of the ultrasonic enzymolysis is 0.3-0.5w/cm 2 The frequency is 20-25kHz.
3. The method for recycling ultrasonic enzymolysis of dead livestock as claimed in claim 2, wherein in the step (7), the compound biological enzyme is a mixture of papain, alkaline protease, neutral protease, acid protease, bromelain and lipase.
4. The method for ultrasonic enzymolysis and recycling of dead livestock as defined in claim 3, wherein in the step (8), the filter aid is a mixture of diatomite and perlite.
5. The ultrasonic enzymolysis recycling method for livestock dying of illness according to claim 4, wherein the step (9) and the step (10) further comprise the step of temporarily storing the filtrate for 0.5-1h.
6. The method for ultrasonic enzymolysis and recycling of livestock dying of illness according to claim 5, wherein the step (11) further comprises the step of keeping the prepared product in a dark place and sealing.
7. The ultrasonic enzymolysis recycling method for dead livestock as defined in claim 6, wherein in the step (1), the particle size of the dead livestock after the crushing treatment is 5-8cm.
8. The ultrasonic enzymolysis recycling method for dead livestock as claimed in claim 7, wherein in the step (2), the particle size of the dead livestock after meat mincing treatment is 4-8mm.
9. A system for implementing the method for recycling ultrasonic enzymolysis of dead livestock according to any one of claims 1-8, which is characterized by comprising a grinding mill, a meat grinder, a mixing tank, a hydrolysis tank, a horizontal centrifuge, a disc centrifuge, a grease transfer barrel, an ultrasonic enzymolysis tank, a filtration-assisting mixing tank, a plate-and-frame filter press, a ceramic filter and a blending tank;
the discharging end of the grinding machine is connected with the meat grinder; the discharging end of the meat grinder is connected with the mixing tank; the discharging end of the mixing tank is connected with the hydrolysis tank; the discharging end of the hydrolysis tank is connected with a horizontal centrifuge; the discharging end of the horizontal centrifuge comprises a solid-phase material discharging end and a liquid-phase material discharging end; the solid-phase material discharging end is connected with an ultrasonic enzymolysis tank; the liquid phase material discharging end is connected with a disc type centrifuge; the discharge end of the disc centrifuge comprises a water phase material discharge end and an oil phase material discharge end; the oil phase material discharging end is connected with the oil transfer barrel; the water phase material discharging end is connected with an ultrasonic enzymolysis tank; the discharge end of the ultrasonic enzymolysis tank is connected with a filtering-assisting mixing tank; the discharging end of the auxiliary filtering mixing tank is connected with a plate-and-frame filter press; the discharging end of the plate-and-frame filter press comprises a filter material discharging end and a filtrate discharging end; the filtrate discharge end is connected with a ceramic filter; the discharge end of the ceramic filter is connected with the blending tank.
10. The system for realizing the ultrasonic enzymolysis recycling method for livestock dying of illness according to claim 9, further comprising a temporary storage tank and a sealing tank; the temporary storage tank is arranged between the filtrate discharge end and the ceramic filter, and the sealing tank is connected with the discharge end of the blending tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310992785.4A CN116748279A (en) | 2023-08-08 | 2023-08-08 | Ultrasonic enzymolysis recycling method and system for livestock dying of illness |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310992785.4A CN116748279A (en) | 2023-08-08 | 2023-08-08 | Ultrasonic enzymolysis recycling method and system for livestock dying of illness |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116748279A true CN116748279A (en) | 2023-09-15 |
Family
ID=87961167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310992785.4A Pending CN116748279A (en) | 2023-08-08 | 2023-08-08 | Ultrasonic enzymolysis recycling method and system for livestock dying of illness |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116748279A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797567A (en) * | 2010-03-24 | 2010-08-11 | 北京泰克美科技有限公司 | Processor for ultrasonic wave with mixed wavelengths |
| KR101270665B1 (en) * | 2013-02-21 | 2013-06-03 | 코리아워터텍 주식회사 | Apparatus and method for resource conversion of animal waste |
| CN105268724A (en) * | 2015-11-06 | 2016-01-27 | 中国科学院城市环境研究所 | Dead livestock resource utilization method and device |
| CN106563686A (en) * | 2016-10-21 | 2017-04-19 | 河南农业大学 | A method of subjecting livestock and poultry died of disease to ultrasonic enzymatic hydrolysis |
| CN107363071A (en) * | 2017-06-22 | 2017-11-21 | 烟台大学 | A kind of method of dead livestock and poultry recycling treatment |
| CN110407623A (en) * | 2019-07-23 | 2019-11-05 | 武汉益锦祥生物环保有限公司 | A method of high-valued fertilizer is prepared based on dead livestock and poultry harmless treatment |
| CN112998118A (en) * | 2021-03-12 | 2021-06-22 | 时代生物科技（深圳）有限公司 | Method for preparing hypoallergenic milk protein peptide by physical and chemical combination |
| CN115197022A (en) * | 2022-07-19 | 2022-10-18 | 深圳康民农业生物科技发展有限公司 | Organic fertilizer micron-coated slow release method |
| CN116140338A (en) * | 2023-04-21 | 2023-05-23 | 江苏北斗星环保股份有限公司 | Beasts and birds that die of illness dry system enzymolysis innocent treatment system |
-
2023
- 2023-08-08 CN CN202310992785.4A patent/CN116748279A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797567A (en) * | 2010-03-24 | 2010-08-11 | 北京泰克美科技有限公司 | Processor for ultrasonic wave with mixed wavelengths |
| KR101270665B1 (en) * | 2013-02-21 | 2013-06-03 | 코리아워터텍 주식회사 | Apparatus and method for resource conversion of animal waste |
| CN105268724A (en) * | 2015-11-06 | 2016-01-27 | 中国科学院城市环境研究所 | Dead livestock resource utilization method and device |
| CN106563686A (en) * | 2016-10-21 | 2017-04-19 | 河南农业大学 | A method of subjecting livestock and poultry died of disease to ultrasonic enzymatic hydrolysis |
| CN107363071A (en) * | 2017-06-22 | 2017-11-21 | 烟台大学 | A kind of method of dead livestock and poultry recycling treatment |
| CN110407623A (en) * | 2019-07-23 | 2019-11-05 | 武汉益锦祥生物环保有限公司 | A method of high-valued fertilizer is prepared based on dead livestock and poultry harmless treatment |
| CN112998118A (en) * | 2021-03-12 | 2021-06-22 | 时代生物科技（深圳）有限公司 | Method for preparing hypoallergenic milk protein peptide by physical and chemical combination |
| CN115197022A (en) * | 2022-07-19 | 2022-10-18 | 深圳康民农业生物科技发展有限公司 | Organic fertilizer micron-coated slow release method |
| CN116140338A (en) * | 2023-04-21 | 2023-05-23 | 江苏北斗星环保股份有限公司 | Beasts and birds that die of illness dry system enzymolysis innocent treatment system |
Non-Patent Citations (1)
| Title |
|---|
| 罗永康: "《食物蛋白质控制酶解技术》", vol. 1, 中国轻工业出版社, pages: 355 - 14 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2276795B1 (en) | Processing biomass | |
| DK2276795T3 (en) | BIOMASS PREPARATION | |
| KR101502376B1 (en) | Manufacturing apparatus of the amino acid liquid fertilizer and protein feed using slaughter blood | |
| US3041174A (en) | Process for treating oil-containing animal material, such as fish and fish offal | |
| CN107981028B (en) | Preparation method of easy-to-preserve small-peptide-rich small marine trash fish enzymatic whole fish paste | |
| CN104397857A (en) | Concentrate automatic processing device and concentrate preparation method | |
| CN101613731A (en) | A kind of leftovers of ictalurus punctatus is recycled technology | |
| KR20140111759A (en) | Manufacturing method of the amino acid liquid fertilizer and protein feed using slaughter blood | |
| CN107431230A (en) | Using the food refuse installation for fermenting and animal manure Fertilizer Transformed device of edaphon, it is suitable for its biological field generation device, is suitable for its microorganism battery and is suitable for its microorganism capacitor | |
| CN104366680A (en) | Automated chicken fodder processing device and chicken fodder preparation method | |
| KR20160021515A (en) | A poultry feed manufacturing method with healthy functionality and the device, removing the smell of the starfish | |
| JP2001511008A (en) | Method and apparatus for collecting, digesting and dehydrating krill hydrolyzate and drying and treating such hydrolyzate | |
| JPH10500004A (en) | Method and apparatus for krill collection and processing | |
| KR101446180B1 (en) | Recycling system for food garbage | |
| CN116748279A (en) | Ultrasonic enzymolysis recycling method and system for livestock dying of illness | |
| CN104996726B (en) | Utilize the method for the flat angle stratiomyiid larva production functional feedstuff additive of speck | |
| CN107811162A (en) | A kind of de- bitter enzymolysis of small oppossum shrimp of marine products prepares method and the application of the full shrimp slurry of easy preservation | |
| EP0148143B1 (en) | A method for increasing the milk yield of dairy cows by means of a feed, a method for preparing the feed and an apparatus for carrying out said method | |
| CN101720905B (en) | Flavor yeast albumen powder and preparation method thereof | |
| KR101655404B1 (en) | Method for producing functional egg having increased amino acid content, and functional egg having increased amino acid content produced by the same | |
| WO2014017945A1 (en) | Zero-waste method for producing ethanol and apparatus for implementing same | |
| RU2372790C1 (en) | Method of obtaining fodder based on protein hydrolysate | |
| RU2173532C1 (en) | Feed fish flour production method | |
| RU2557191C1 (en) | Method of production of biological preparation for feed production | |
| CN107821728B (en) | Preparation method and application of whole fish paste rich in small peptides prepared by enzymolysis of small marine fishes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |