CN116747180A

CN116747180A - Moisturizing, skin-refreshing and anti-aging composition and preparation method and application thereof

Info

Publication number: CN116747180A
Application number: CN202310825140.1A
Authority: CN
Inventors: 陈潇
Original assignee: Shanghai Keyan Biotechnology Co ltd
Current assignee: Shanghai Keyan Biotechnology Co ltd
Priority date: 2023-07-06
Filing date: 2023-07-06
Publication date: 2023-09-15

Abstract

The invention relates to A61K8, in particular to a moisturizing, skin-refreshing and anti-aging composition, a preparation method and application thereof. The moisturizing and skin-rejuvenating and anti-aging composition at least comprises a yeast fermentation product lysate and a rice grain extract; the mass ratio of the lysate of the fermentation product of the saccharomyces cerevisiae to the rice grain extract is (0.6-1.9): (0.4-0.9). The composition prepared by the invention is used for preparing essence, and has good stability and moisture retention performance.

Description

Moisturizing, skin-refreshing and anti-aging composition and preparation method and application thereof

Technical Field

The invention relates to A61K8, in particular to a moisturizing, skin-refreshing and anti-aging composition, a preparation method and application thereof.

Background

Cells on the skin of the human body undergo aging and death during the growth process, and the final product of the cells on the outermost layer is cutin. When the produced cutin is excessive, the skin becomes dull and accompanied by acne, wrinkles, desquamation, etc. In general, when the metabolism of keratin progresses slowly or the skin is in an unhealthy state, the skin is not only dull, the moisture retention is lowered, but also the care product is hardly absorbed, and it is necessary to remove the old keratin peeled off earlier by some means.

Chinese patent CN202110701425 discloses a facial skin cutin renewal promoter and its application, comprising 1-10% of pomegranate fruit fermentation product extract and 0.1-5% of hydroxyethyl piperazine ethane sulfonic acid, the molecular weight of the extract extracted from the pomegranate fruit after lactobacillus fermentation is smaller. Is more beneficial to softening the horny layer and promoting the falling of the horny layer. The proportion of the nutritional components and the anti-nutritional components in the pomegranate fruits can be changed by fermentation and extraction, the biological activity of the product is improved, the hydroxyethyl piperazine ethane sulfonic acid can provide a more stable buffer environment, the maintenance of the biological enzyme structure and the functions of the active components is facilitated, no fruit acid substances are contained, the system requirement on the formula is low, the method is suitable for products with pH value of 4-6.5, the skin feel is good, the sensitive muscles are not stimulated, and the skin-whitening and skin-refreshing agent is a mild cutin renewal promoter, and has better skin barrier repairing function and skin whitening and refreshing effects. However, this solution only focuses on the turnover of keratin, which may lead to dry skin and skin peeling problems.

Disclosure of Invention

In order to solve the above problems, the first aspect of the present invention provides a moisturizing, rejuvenating, anti-aging composition comprising at least a fermentation product lysate of Saccharomyces cerevisiae and a rice (ORYZA SATIVA) grain extract; the mass ratio of the lysate of the fermentation product of the saccharomyces cerevisiae to the rice grain extract is (0.6-1.9): (0.4-0.9).

In order to improve the moisture retention of the composition, it is preferable that the composition further comprises one or more combinations of white willow (SALIX ALBA) bark extract, japanese evening primrose (PRUNUS LANNESIANA) flower extract, ginkgo biloba l root extract, lycium barbarum l. Fruit extract, chrysanthemums (chrysanthemumindicum) extract.

Preferably, the composition further comprises white willow bark extract and japanese evening primrose extract. By adding white willow bark extract and Japanese evening primrose extract, the skin refreshing and antiaging effects of the composition are improved, and the moisture keeping effect of the composition is improved.

Preferably, the yeast fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.6-1.9): (0.4-0.9): (0.05-0.16): (0.05-0.15).

In order to improve the stability of the composition, preferably, the two yeast fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.8-1.6): (0.5-0.85): (0.08-0.15): (0.08-0.13). The compatibility of the two-split yeast fermentation product lysate, rice grain extract, white willow bark extract and Japanese evening primrose extract in the weight ratio is good, and the coaction of active ingredients in the system can be further improved, so that the moisturizing performance of the composition is improved.

In order to improve the stability of the composition and at the same time improve the moisturization durability of the composition, preferably, the yeast fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.8-1.5): (0.6-0.75): (0.12-0.15): (0.08-0.10). The composition obtained from the fermentation product lysate of the two-split yeast, the rice grain extract, the white willow bark extract and the Japanese evening primrose extract in the weight ratio is easier to be typed and absorbed by skin, thereby achieving the long-acting moisturizing effect.

Preferably, the yeast fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is 1.0:0.7:0.15:0.1.

preferably, the preparation method of the rice grain extract comprises the following steps: taking 100g of fermented rice grain raw material, drying in a baking oven at 35 ℃ for 12 hours, taking out, performing ultrasonic extraction by using deionized water with the mass being 2 times, performing ultrasonic extraction for 40 minutes at the ultrasonic temperature of 70 ℃, and filtering to obtain the rice grain extract.

The second aspect of the invention provides a method for preparing a moisturizing, skin rejuvenating and anti-aging composition, comprising the steps of: mixing the two-split yeast fermentation product lysate, rice grain extract, white willow bark extract and Japanese evening primrose extract according to weight ratio, loading into a ball milling tank, adding zirconium beads, ball milling for 20min, and taking out to obtain the moisturizing and skin refreshing composition.

The third aspect of the invention provides application of a moisturizing, rejuvenating and anti-aging composition, which can be applied to skin care products.

Preferably, the skin care product comprises essence, essence water, face cream, eye mask, face cleansing cream, cleansing water, cleansing cream, shampoo, bath lotion, hair conditioner, facial mask, foundation solution and gel.

Preferably, the skin care product is essence.

The essence comprises the following raw materials in percentage by mass: 2% of moisturizing, skin-refreshing and anti-aging composition, 15% of glycerol, 5% of water-soluble chitosan, 5% of propylene glycol, 3% of sodium hyaluronate and the balance of water.

The preparation method of the essence comprises the following steps: the raw materials are uniformly mixed according to the mass percentage.

The beneficial effects are that:

(1) The two-split yeast fermentation product lysate and the rice grain extract act together, so that the metabolism of cutin can be enhanced, and skin cell peroxidation can be inhibited, and an anti-aging effect is achieved.

(2) The composition of the present invention further comprises white willow bark extract and japanese evening primrose extract. By adding white willow bark extract and Japanese evening primrose extract, the skin refreshing and antiaging effects of the composition are improved, and the moisture keeping effect of the composition is improved.

(3) The invention defines a lysate of a fermentation product of Saccharomyces cerevisiae: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.8-1.6) to (0.5-0.85) to (0.08-0.15) to (0.08-0.13), and the stability of the composition is improved.

(4) The invention defines a lysate of a fermentation product of Saccharomyces cerevisiae: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.8-1.5) to (0.6-0.75) to (0.12-0.15) to (0.08-0.10), and the Japanese evening primrose extract is more easily absorbed by skin, so that the long-acting moisturizing effect is achieved.

(5) The invention adds 2% of moisturizing, skin-refreshing and anti-aging composition into the essence, and remarkably improves the moisturizing performance of the essence.

(6) The moisturizing and skin-refreshing anti-aging composition provided by the invention is subjected to ball milling treatment, so that the stability of the composition is ensured.

Detailed Description

Example 1

This example provides a moisturizing, skin rejuvenating, anti-aging composition comprising: a yeast fermentation product lysate, rice grain extract, white willow bark extract, japanese evening primrose extract;

the two yeast fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is 0.8:0.65:0.14:0.1.

The preparation method of the rice grain extract comprises the following steps: taking 100g of fermented rice grain raw material, drying in a baking oven at 35 ℃ for 12 hours, taking out, performing ultrasonic extraction by using deionized water with the mass being 2 times, performing ultrasonic extraction for 40 minutes at the ultrasonic temperature of 70 ℃, and filtering to obtain the rice grain extract.

A method for preparing a moisturizing skin rejuvenating and anti-aging composition comprises the following steps: mixing the two-split yeast fermentation product lysate, rice grain extract, white willow bark extract and Japanese evening primrose extract according to weight ratio, loading into a ball milling tank, adding zirconium beads, ball milling for 20min, and taking out to obtain the moisturizing and skin refreshing composition.

An application of a moisturizing, skin-refreshing and anti-aging composition is provided, which is applied to essence.

Example 2

This example provides a moisturizing, skin rejuvenating, anti-aging composition, unlike example 1, the two-part yeast fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is 1.0:0.7:0.15:0.1.

A preparation method of a moisturizing, skin-refreshing and anti-aging composition is provided, and specific embodiments of the composition are the same as in example 1.

An application of a moisturizing, skin-refreshing and anti-aging composition is applied to essence, and specific embodiments of the composition are the same as in example 1.

Example 3

This example provides a moisturizing, skin rejuvenating, anti-aging composition, unlike example 2, the two-part yeast fermentation product lysate: rice grain extract: white willow bark extract: the ratio of the Japanese evening primrose extract to the Japanese cherry extract is 2.0:1.0:0.17:0.16.

Example 4

This example provides a moisturizing, skin rejuvenating, anti-aging composition that, unlike example 2, does not add a lysate of a fermentation product of Saccharomyces cerevisiae.

The rice grain extract: white willow bark extract: the weight ratio of Japanese evening primrose extract is 0.7:0.15:0.1

Example 5

This example provides a moisturizing, skin rejuvenating, anti-aging composition, unlike example 2, the two-part yeast fermentation product lysate: rice grain extract: white willow bark extract: the ratio of the Japanese evening primrose extract to the Japanese cherry extract is 2.0:1.0:0.13:0.12.

Example 6

This example provides a moisturizing, rejuvenating, anti-aging composition, the embodiments of which are the same as example 2,

a method for preparing a moisturizing, skin rejuvenating, anti-aging composition, unlike example 2, was not ball milled.

Performance test:

1. the stability test, test conditions and results of the essences prepared in examples 1 to 5 are shown in Table 1.

No change in color, appearance and smell is marked as qualified, and any change in color, appearance and smell is marked as unqualified.

TABLE 1

2. Human skin patch experiments of the essence prepared in example 2.

(1) Test article: the essence prepared in example 1.

(2) Negative control: blank control.

(3) The subject: 32 people total, 32 women, age 20 to 49 years, average age 31.74+ -6.83 years, meet the volunteer inclusion criteria of the subject.

(4) Plaque test method: selecting qualified office test equipment, placing the test object in a patch tester with a volume of about 0.020 mL-0.025 mL by using a closed patch test method, applying the test object to the back of a subject by using a hyposensitization tape externally, removing the test object from the hospital after 24 hours, observing skin reactions respectively after 0.5 hour, 24 hours and 48 hours after removal, and recording the results according to skin reaction grading standards in the existing effective technical specifications, wherein the grading standards are shown in Table 2, and the test results are shown in Table 3.

TABLE 2

TABLE 3 Table 3

3. Moisturizing efficacy of the essence prepared in example 2.

(1) Summary of the method

[ method basis ] [ QB/T4256-2011 chemical product moisturizing potency guide ]

[ test purpose ] evaluation of the Effect of short-term moisturizing of the product by testing the changes in the moisture content of the skin horny layer before and after the use of the sample in a subject

[ method of use ]

Sample name/number: essence prepared in example 1

Test part: inner side of forearm

Frequency of use: single use

The using method comprises the following steps: after the inner side of the arm is cleaned by the soap-based facial cleanser, a quantitative test object is taken and uniformly smeared on the tested area [ subject information ] to be 33 persons, 33 women, the age of 18 to 45 years, and the average 3167+/-7.63

[ detection environment ] temperature 20-22 ℃; humidity: 50% -60% RH

Detection instrument Corneometer@CM825

[ test item ] moisturizing efficacy

(2) Subject screening criteria

Inclusion criteria:

1) Volunteers 18-65 years old with women meeting experimental requirements

2) The basis value of the forearm test area capacitance skin moisture meter is between 15 and 45 (colorimeter Unit, c.u.)

3) The subject is not allergic to the usual cosmetics.

Exclusion criteria

1) Patients with severe systemic diseases, immunodeficiency or autoimmune diseases, the subject site is subjected to skin treatment, cosmetic and other tests that may affect the outcome.

2) Active allergic disease patients

3) The patients with high sensitivity to physical constitutions.

4) Women in lactation or gestation.

5) Hormone drugs and immunosuppressants have been used in the last month

6) The subject was not engaged in other clinical trials now or recently three months

7) The test area has skin characterization of large-area mole marks, scratches, white spots, pigmented nevi, keloids and the like affecting experiments

8) Participate in other clinical trial researchers

9) The test person in charge determines that the test person does not meet the population as the subject of the present test.

Limiting matters:

1) During the test, hot water consumption is prohibited

2) Constant temperature and humidity room capable of prohibiting frequent access

3) Inhibit shielding or touching test sites during testing

Exit criteria:

in the test process, the situation of adverse reaction, no trouble visit or violating a research scheme and other special reasons occurs in the test subject, and if the test subject is not suitable for the situation of continuing the test any more, the test subject is required to exit the test.

(3) Testing and testing procedure

And (3) test design: single trial

Test procedure

1) Subjects of recruiter

2) Signing informed consent

3) Prior to the group entry, subjects were asked a series of questions regarding disease history, health status, etc. based on inclusion and exclusion criteria, etc., and the skin condition at the 3-part of the trial was evaluated for compliance and recorded.

4) The qualified subject of screening cleans the skin of test area first, still sits for 30min in constant temperature and humidity environment, can not drink water and beverage, keeps relaxing, and staff can make the mark of smearing area and blank control area for the inboard of subject's both hands forearm during balancing (size: 3cm x 3 cm) should be randomly distributed in the left and right arm calibration areas to ensure that all product and blank area positions are statistically balanced.

5) The test samples were taken in single passes (2.00.1 g/cm, latex was used to mean that the samples were evenly distributed in the test area and the actual applied sample amount was recorded.

6) After the staff has adjusted the instrument according to the instructions for use, the marked sample areas and the control areas are measured, each area being measured in parallel 3 times. Initial values for each test area (before sample use) were measured and then the skin stratum corneum moisture content of the sample and control areas was determined after set times 4H, 8H.

7) Testing of the same subject must be performed by the same tester using an instrument, and the measurement probe should be cleaned between measurements

8) During the use of the product, as in the case of volunteer skin, the test should be terminated immediately and the volunteer should be properly treated to record the adverse reaction.

Test control

Test part: front inner side

The test indexes are as follows: water content of stratum corneum

Control mode: self control and blank control

Test index

Moisturizing: measurement of skin stratum corneum Water content by Corneometer@CM825 tester

Parameter interpretation: higher values indicate better moisturizing effect, and worse values

Data statistical analysis method

1) Taking data before and after using samples 4H and 8H

2) Data statistics

The SPSS application statistical analysis software is used for carrying out descriptive statistics on the measured values of each test time point, wherein the statistical analysis software comprises a number, a mean value, a standard deviation minimum value, a maximum value and the like. The significance Test of the normal distribution of the data is carried out by using a shape-Wilk Test, sig (double sides) >0.05 samples are in normal distribution, the normal distribution requirement is met, and the statistical analysis is carried out by adopting a t Test method: and if the test data are non-positive too-distributed, adopting a rank sum test method to carry out statistical analysis.

Calculating the difference between the initial values of the sample area and the blank area and the other measured time point measurement values nth hour value = nth hour-initial value

Note that: in this test, n is 4H, 8H

(4) The test results are shown in Table 4

Note that: statistical software SPSS analysis, initial value of vs at n hours, "n.s" means statistically no significant difference P > 0.05; ". Times." indicates significant differences, 0.01.ltoreq.P < 0.05: "x" means 0.001: p is more than or equal to 0.01: ", means P < 0.001.

Claims

1. A moisturizing, rejuvenating and anti-aging composition, which is characterized in that the moisturizing, rejuvenating and anti-aging composition at least comprises a lysate of a fermentation product of a saccharomyces cerevisiae and a rice grain extract; the mass ratio of the lysate of the fermentation product of the saccharomyces cerevisiae to the rice grain extract is (0.6-1.9): (0.4-0.9).

2. The moisturizing, skin rejuvenating, anti-aging composition according to claim 1, further comprising one or more combinations of white willow bark extract, japanese evening primrose extract, ginkgo root extract, lycium barbarum extract, golden chamomile extract.

3. The moisturizing, skin rejuvenating, anti-aging composition according to claim 2, wherein the yeast bifidus fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.6-1.9): (0.4-0.9): (0.05-0.16): (0.05-0.15).

4. A moisturizing, skin rejuvenating, anti-aging composition according to claim 3, wherein the yeast bifidus fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.8-1.6): (0.5-0.85): (0.08-0.15): (0.08-0.13).

5. The moisturizing, skin rejuvenating, anti-aging composition according to claim 4, wherein the yeast bifidus fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is (0.8-1.5): (0.6-0.75): (0.12-0.15): (0.08-0.10).

6. The moisturizing, skin rejuvenating, anti-aging composition according to claim 5, wherein the yeast bifidus fermentation product lysate: rice grain extract: white willow bark extract: the weight ratio of the Japanese evening primrose extract is 1.0:0.7:0.15:0.1.

7. the moisturizing, skin rejuvenating and anti-aging composition according to claim 6, wherein the rice grain extract is prepared by the following steps: taking 100g of fermented rice grain raw material, drying in a baking oven at 35 ℃ for 12 hours, taking out, performing ultrasonic extraction by using deionized water with the mass being 2 times, performing ultrasonic extraction for 40 minutes at the ultrasonic temperature of 70 ℃, and filtering to obtain the rice grain extract.

8. A process for preparing a moisturizing, skin rejuvenating, anti-aging composition according to any one of claims 1 to 7, comprising the steps of:

mixing the two-split yeast fermentation product lysate, rice grain extract, white willow bark extract and Japanese evening primrose extract according to weight ratio, loading into a ball milling tank, adding zirconium beads, ball milling for 20min, and taking out to obtain the moisturizing and skin refreshing composition.

9. Use of a moisturizing, rejuvenating, anti-aging composition according to any one of claims 1 to 7, wherein the moisturizing, rejuvenating, anti-aging composition is applicable in skin care products.

10. The use of a moisturizing, skin rejuvenating, anti-aging composition according to claim 9, wherein the skin care product comprises a serum, a cream, an eye mask, a face cleansing, a make-up remover, a shampoo, a body wash, a conditioner, a mask, a foundation, a gel.