CN114306194A - Freckle-removing cosmetic composition containing umbilical cord blood extract, freckle-removing cream and preparation method thereof - Google Patents
Freckle-removing cosmetic composition containing umbilical cord blood extract, freckle-removing cream and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of cosmetics, and particularly discloses a freckle-removing cosmetic composition containing an umbilical cord blood extract, a freckle-removing cream and a preparation method of the freckle-removing cream. The freckle-removing cosmetic composition comprises the following raw materials in parts by weight: 5-15 parts of umbilical cord extract, 1-10 parts of hydrolyzed placenta extract, 0.5-1.5 parts of arbutin, 4-10 parts of pomegranate exosome and 2-6 parts of giant knotweed exosome. According to the invention, five components of the umbilical cord extract, the hydrolyzed placenta extract, arbutin, the pomegranate exosome and the giant knotweed exosome are reasonably matched, so that the prepared freckle-removing cosmetic composition can play a role in synergy, can achieve strong freckle-removing and whitening effects, is mild and non-irritant, and has high product safety.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a freckle-removing cosmetic composition containing an umbilical cord blood extract, freckle-removing cream and a preparation method thereof.
Background
Mottle refers to a spot of a different color from the surrounding. Including freckle, black spot, chloasma and senile plaque, and belongs to skin diseases with dyschromatosis. A common facial brown or melanotic and damaged skin disease caused by the increase of skin melanin is frequently generated at cheek and forehead parts, aggravates after solarization, is commonly seen in women, and is related to pregnancy and long-term menstrual disorder.
Various methods for inhibiting the production of melanin, such as inhibiting the activity of tyrosinase, scavenging active oxygen or free radicals, reducing ultraviolet irradiation, preventing the occurrence of oxidation reaction, etc., are effective methods for reducing the formation of melanin. At present, people can add various whitening and freckle-removing components into cosmetics to achieve the effect of reducing or eliminating freckles. The commonly used whitening and freckle-removing components comprise arbutin and derivatives thereof, fruit acids, vitamin C and derivatives thereof and the like, but the whitening and freckle-removing components have stronger irritation and are easy to interfere with the normal physiological metabolism function of the skin.
Broxmeyer first proved in 1988 that cord blood is rich in hematopoietic stem cells, which are a cell population with self-renewal, high proliferation and multi-directional differentiation potential, and are "progenitor cells" and "universal cells" forming various tissues and organs of human body. Can be divided into embryonic stem cells and adult stem cells according to the development stage of the cell, and can be divided into totipotent stem cells, pluripotent stem cells and unipotent stem cells according to the size of differentiation potential. The stem cells not only can generate or secrete a large amount of bioactive factors, but also contain rich bioactive substances, and the bioactive factors or substances of the stem cells can effectively regulate and control the cell signal conduction of organisms and activate the stem cells of the human bodies, so that cells with body injury, pathological changes and aging can be physiologically repaired or replaced.
Therefore, the bioactive factors generated by the secretion of the stem cells or bioactive substances in the stem cells are utilized to activate the stem cells of the organism, and then the cells with body injury, pathological changes and aging are physiologically repaired or replaced by the stem cells, so that the stem cells have wide application prospects in the fields of disease prevention and treatment, health care and beauty.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a freckle-removing cosmetic composition containing an umbilical cord blood extract, a freckle-removing cream and a preparation method thereof. The composition has the advantages of low irritation, good speckle fading effect, high safety and the like.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a freckle-removing cosmetic composition containing an umbilical cord blood extract, which comprises the following raw materials in parts by weight:
5-15 parts of umbilical cord extract, 1-10 parts of hydrolyzed placenta extract, 0.5-1.5 parts of arbutin, 4-10 parts of pomegranate exosome and 2-6 parts of giant knotweed exosome.
The freckle-removing cosmetic composition disclosed by the invention is prepared by compounding the plant exosomes and the traditional whitening components, so that the problems of poor safety and high irritation of the traditional whitening and freckle-removing active components are solved, the heat resistance, oxidation resistance and ultraviolet resistance of the whitening and freckle-removing active components can be improved, and the whitening and freckle-removing cosmetic composition is safer, more stable and more efficient than other whitening and freckle-removing components.
Arbutin can inhibit tyrosinase activity, thereby inhibiting melanin production. In the invention, the umbilical cord extract, the hydrolyzed placenta extract, the arbutin, the pomegranate exosome and the giant knotweed exosome are reasonably matched to play a role in synergy, so that the effects of stronger freckle removing and whitening can be achieved, and the patch is mild and has no stimulation.
As a preferred embodiment of the spot-removing cosmetic composition, the spot-removing cosmetic composition comprises the following raw materials in parts by weight:
5-12 parts of umbilical cord extract, 1-8 parts of hydrolyzed placenta extract, 0.5-1.2 parts of arbutin, 4-8 parts of pomegranate exosome and 2-5 parts of giant knotweed exosome.
When the raw materials are in the parts by weight, the prepared freckle-removing cosmetic composition has a better freckle-removing and whitening effect, and the problems of poor safety, high irritation and the like of an arbutin component are better solved, so that the comprehensive performance is strong.
More preferably, the freckle-removing cosmetic composition comprises the following raw materials in parts by weight:
8 parts of umbilical cord extract, 5 parts of hydrolyzed placenta extract, 1 part of arbutin, 6 parts of pomegranate exosome and 4 parts of giant knotweed exosome.
As a preferred embodiment of the spot-removing cosmetic composition of the present invention, the preparation method of the pomegranate exosome or the giant knotweed exosome comprises the following steps:
1) grinding pomegranate or giant knotweed rhizome, adding PBS buffer solution, filtering, centrifuging, and taking supernatant;
2) and (4) carrying out ultracentrifugation on the obtained supernatant, taking the precipitate, carrying out heavy suspension on the precipitate by using a buffer solution, and taking the precipitate.
The prepared pomegranate exosome, the prepared giant knotweed exosome and arbutin are compounded according to a specific proportion, so that the irritation of arbutin can be reduced, and the phenomenon of skin allergy can be reduced.
As a preferred embodiment of the spot-removing cosmetic composition according to the present invention, the rotation speed of the centrifugation in the step 1) is 8000rpm to 9500 rpm; the rotating speed of the centrifugation in the step 1) is 8500rpm-10000 rpm.
The invention also provides application of the freckle-removing cosmetic composition in preparation of water, milk, essence and cream.
In addition, the invention also provides a freckle-removing cream which comprises the following components in percentage by weight:
the freckle-removing cosmetic composition comprises 1-3% of the freckle-removing cosmetic composition, 0.2-1% of a skin conditioner, 5-15% of a humectant, 0.2-1% of an emulsifier, 0.5-1% of an antioxidant and the balance of water.
The freckle-removing cream prepared by the invention has strong whitening and freckle-removing effects, is safe, has no stimulation, has no cytotoxicity, can perform all-around whitening and freckle removal from the whole process of melanin generation and metabolism, and is not easy to rebound.
As a preferred embodiment of the spot removing cream of the present invention, the skin conditioner is at least one of dipotassium glycyrrhizinate and allantoin; the humectant is hydrogenated polyisobutene; the emulsifier is candelilla wax or cetostearyl alcohol; the antioxidant is p-hydroxyacetophenone.
The invention also provides a preparation method of the freckle removing cream, which comprises the following steps:
s1, mixing the humectant, the emulsifier, the antioxidant and water to obtain a water-phase material;
s2, cooling the water phase material, adding the freckle-removing cosmetic composition and the skin conditioner, mixing, and cooling to obtain the freckle-removing cream.
Compared with the prior art, the invention has the following beneficial effects:
the umbilical cord extract, the hydrolyzed placenta extract, the arbutin, the pomegranate exosome and the giant knotweed exosome are reasonably matched, so that the prepared freckle-removing cosmetic composition can play a role in synergy, can achieve strong freckle-removing and whitening effects, and is mild and non-irritant; meanwhile, the pomegranate exosome, the giant knotweed exosome and the arbutin are compounded, so that the problems of poor safety, high irritation and the like of the arbutin component can be solved, the irritation of the composition to the skin is reduced, and the safety of the product is improved.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
In the following examples and comparative examples, the experimental methods used were conventional ones unless otherwise specified, and the materials, reagents and the like used were commercially available ones unless otherwise specified.
The animal umbilical cord extract is derived from Shenzhen RigJi Biotech limited, CAS number is 98654-16-1; the hydrolyzed placenta extract is obtained from Hangzhou Beirong Biotech limited.
In the following examples and comparative examples, arbutin, skin conditioner, moisturizer, emulsifier, antioxidant are all the same commercial products.
The preparation method of the pomegranate exosome and the giant knotweed exosome comprises the following steps: grinding pomegranate or giant knotweed rhizome by a tissue grinder, dissolving with PBS (phosphate buffer solution) in a ratio of 1:100, filtering by gauze, centrifuging at 9000rpm for 60min, collecting supernatant, transferring the collected supernatant to an ultracentrifuge tube, accurately balancing under the condition that the error is less than 0.001g, putting the ultracentrifuge tube into a precooled ultracentrifuge, and centrifuging at the temperature of 4 ℃ and the temperature of 9500 Xg for 70 min. The supernatant was discarded, the pellet was retained and resuspended in PBS, and after equilibration, centrifuged again at 9500 Xg for 70min at 4 ℃. After completion, the pellet was collected, resuspended in a small amount of PBS, and stored in an ultra-low temperature refrigerator.
Example 1 Spot-removing cosmetic composition
A freckle-removing cosmetic composition is composed of the following raw materials: 5 parts of umbilical cord extract, 1 part of hydrolyzed placenta extract, 0.5 part of arbutin, 4 parts of pomegranate exosome and 2 parts of giant knotweed exosome.
Example 2 Spot-removing cosmetic composition
A freckle-removing cosmetic composition is composed of the following raw materials: 12 parts of umbilical cord extract, 8 parts of hydrolyzed placenta extract, 1.2 parts of arbutin, 8 parts of pomegranate exosome and 5 parts of giant knotweed exosome.
Example 3 Spot-removing cosmetic composition
A freckle-removing cosmetic composition is composed of the following raw materials: 15 parts of umbilical cord extract, 10 parts of hydrolyzed placenta extract, 1.5 parts of arbutin, 10 parts of pomegranate exosome and 6 parts of giant knotweed exosome.
Example 4 Spot-removing cosmetic composition
A freckle-removing cosmetic composition is composed of the following raw materials: 8 parts of umbilical cord extract, 5 parts of hydrolyzed placenta extract, 1 part of arbutin, 6 parts of pomegranate exosome and 4 parts of giant knotweed exosome.
Example 5 Spot-removing cream
The freckle-removing cream comprises the following components in percentage by weight:
the freckle-removing cosmetic composition prepared in example 1 comprises 2% of dipotassium glycyrrhizinate, 12% of hydrogenated polyisobutene, 0.6% of candelilla wax, 0.5% of p-hydroxyacetophenone and the balance of water.
The preparation method of the freckle removing cream comprises the following steps:
s1, mixing the hydrogenated polyisobutene, candelilla wax, p-hydroxyacetophenone and water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material; preserving the heat of the water-phase material for 20 minutes for sterilization, starting cooling water, and continuously stirring the water-phase material while the temperature of the cooling water is 25 ℃;
s2, cooling the water phase material to 45 ℃, adding the freckle-removing cosmetic composition prepared in the embodiment 1 and dipotassium glycyrrhizinate, mixing, and cooling to obtain the freckle-removing cream.
Example 6 Spot removing cream
The freckle-removing cream comprises the following components in percentage by weight:
the freckle-removing cosmetic composition prepared in example 2%, dipotassium glycyrrhizinate 0.5%, hydrogenated polyisobutene 12%, candelilla wax 0.6%, p-hydroxyacetophenone 0.5%, and the balance of water.
The preparation method of the freckle removing cream comprises the following steps:
s1, mixing the hydrogenated polyisobutene, candelilla wax, p-hydroxyacetophenone and water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material; preserving the heat of the water-phase material for 20 minutes for sterilization, starting cooling water, and continuously stirring the water-phase material while the temperature of the cooling water is 25 ℃;
s2, cooling the water phase material to 45 ℃, adding the freckle-removing cosmetic composition prepared in the embodiment 2 and the dipotassium glycyrrhizinate, mixing, and cooling to obtain the freckle-removing cream.
Example 7 Spot removing cream
The freckle-removing cream comprises the following components in percentage by weight:
the spot-removing cosmetic composition prepared in example 3 includes 2% of dipotassium glycyrrhizinate, 12% of hydrogenated polyisobutene, 0.6% of candelilla wax, 0.5% of p-hydroxyacetophenone, and the balance of water.
The preparation method of the freckle removing cream comprises the following steps:
s1, mixing the hydrogenated polyisobutene, candelilla wax, p-hydroxyacetophenone and water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material; preserving the heat of the water-phase material for 20 minutes for sterilization, starting cooling water, and continuously stirring the water-phase material while the temperature of the cooling water is 25 ℃;
s2, cooling the water phase material to 45 ℃, adding the freckle-removing cosmetic composition prepared in the embodiment 3 and the dipotassium glycyrrhizinate, mixing, and cooling to obtain the freckle-removing cream.
Example 8 Spot removing cream
The freckle-removing cream comprises the following components in percentage by weight:
the spot-removing cosmetic composition prepared in example 4 comprises 2% of dipotassium glycyrrhizinate, 12% of hydrogenated polyisobutene, 0.6% of candelilla wax, 0.5% of p-hydroxyacetophenone, and the balance of water.
The preparation method of the freckle removing cream comprises the following steps:
s1, mixing the hydrogenated polyisobutene, candelilla wax, p-hydroxyacetophenone and water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material; preserving the heat of the water-phase material for 20 minutes for sterilization, starting cooling water, and continuously stirring the water-phase material while the temperature of the cooling water is 25 ℃;
s2, cooling the water phase material to 45 ℃, adding the freckle-removing cosmetic composition prepared in the embodiment 4 and the dipotassium glycyrrhizinate, mixing, and cooling to obtain the freckle-removing cream.
Example 9 Spot removing cream
The freckle-removing cream comprises the following components in percentage by weight:
the spot-removing cosmetic composition prepared in example 3 includes 1% of dipotassium glycyrrhizinate, 5% of hydrogenated polyisobutene, 0.2% of candelilla wax, 0.8% of p-hydroxyacetophenone, and the balance of water.
The preparation method of the freckle removing cream comprises the following steps:
s1, mixing the hydrogenated polyisobutene, candelilla wax, p-hydroxyacetophenone and water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material; preserving the heat of the water-phase material for 20 minutes for sterilization, starting cooling water, and continuously stirring the water-phase material while the temperature of the cooling water is 25 ℃;
s2, cooling the water phase material to 45 ℃, adding the freckle-removing cosmetic composition prepared in the embodiment 3 and the dipotassium glycyrrhizinate, mixing, and cooling to obtain the freckle-removing cream.
Example 10 Spot-removing cream
The freckle-removing cream comprises the following components in percentage by weight:
the spot-removing cosmetic composition prepared in example 3%, dipotassium glycyrrhizinate 1%, hydrogenated polyisobutene 15%, candelilla wax 1%, p-hydroxyacetophenone 1%, and the balance of water.
The preparation method of the freckle removing cream comprises the following steps:
s1, mixing the hydrogenated polyisobutene, candelilla wax, p-hydroxyacetophenone and water, heating to 80-85 ℃, and continuously stirring to fully and uniformly stir the components to obtain a water-phase material; preserving the heat of the water-phase material for 20 minutes for sterilization, starting cooling water, and continuously stirring the water-phase material while the temperature of the cooling water is 25 ℃;
s2, cooling the water phase material to 45 ℃, adding the freckle-removing cosmetic composition prepared in the embodiment 3 and the dipotassium glycyrrhizinate, mixing, and cooling to obtain the freckle-removing cream.
Comparative example 1
Compared with the example 7, the difference is that the composition does not contain pomegranate exosome and giant knotweed exosome, and the rest raw materials and content are the same as the example 7.
Comparative example 2
Compared with example 7, the difference is that the composition does not contain pomegranate exosome, and the rest raw materials and the parts by weight are the same as example 7.
Comparative example 3
Compared with the example 7, the difference is that the composition does not contain the polygonum cuspidatum exosome, and the rest raw materials and content are the same as the example 7.
Comparative example 4
Compared with example 7, the difference is that the weight part of the red pomegranate exosome in the composition is 15 parts, and the rest raw materials and the content are the same as those in example 7.
Comparative example 5
The difference from example 7 is only that no umbilical cord extract is contained, and the remaining raw materials and contents are the same as those of example 7.
Test example I, safety test
The skin irritation of the products prepared in examples 5-10 and comparative examples 1-5 was tested according to the skin irritation/corrosion test method of the cosmetic safety technical Specification 2015 edition, 8 New Zealand rabbits were taken from each group, the hairs on the backs of the New Zealand rabbits were scraped off, and after the spot-removing cream was applied to the test skin of the New Zealand rabbits several times, the test results are shown in Table 1 below.
The skin irritation intensity is evaluated by an integral mean value, and the reference indexes are as follows: the integral mean value is 0-0.5 (less than or equal to 0.5) and has no stimulation; slightly stimulating the integration mean value of 0.5-2.0 (less than or equal to 2.0); stimulating in an integral average value of 2.0-6.0 (less than or equal to 6.0); the integral mean value is 6.0-8.0 (less than or equal to 8.0) and strong in irritation.
TABLE 1
As can be seen from the data in table 1, the spot removing cream prepared in examples 5 to 10 was non-irritant to the skin irritation intensity in the multiple skin irritation tests according to the criteria judged by the skin irritation/corrosion test method of the cosmetic safety technical code 2015 edition. The freckle removing cream prepared in the comparative example 1 does not contain pomegranate exosome and giant knotweed exosome at the same time, and the freckle removing cream has strong irritation to skin, so that the compounding of the pomegranate exosome and the giant knotweed exosome can regulate the irritation of arbutin to skin, and reduce the phenomena of skin allergy and the like.
The spot removing cream prepared in the comparative examples 2-3 lacks pomegranate exosomes or polygonum cuspidatum exosomes and has small irritation to skin, which shows that the synergistic effect of the pomegranate exosomes and the polygonum cuspidatum exosomes can improve the problems of poor safety and large irritation of the traditional whitening and spot removing active ingredients.
Test example two, evaluation of human body irritation
The freckle-removing cream prepared in the examples 5-10 and the comparative examples 1-3 is used for a human body irritation test, and the specific experimental method is as follows:
1) selecting 90 subjects with obvious facial stains, dividing the subjects into 8 groups, and 10 subjects in each group, wherein the subjects clean the whole face;
2) the face of 8 groups of subjects was tested separately for facial mottle, one for each of examples 5-10 and comparative examples 1-3;
3) using for 15 minutes, and self-detecting heat sensation, stabbing pain sensation and redness;
4) the number of people with product discomfort was compared as shown in table 2 below.
TABLE 2
As can be seen from the data in Table 2, the spot removing cream prepared in examples 5-10 has no irritation to skin, mild performance and high product safety.
The freckle removing cream prepared in the comparative example 1 does not contain pomegranate exosome and giant knotweed exosome at the same time, has stronger stimulation to skin, and the skin of part of subjects is accompanied with symptoms of heat sensation, stabbing pain, redness and the like.
The freckle removing cream prepared by the comparative examples 2-3 lacks pomegranate exosomes or polygonum cuspidatum exosomes and has small irritation to skin.
Test example three, patch test
The test method comprises the following steps: a patch test method for detecting the safety of a human body adopts a 48h closed patch test.
20 mu g of each of the freckle-removing creams prepared in examples 5 to 10 was weighed into a freckle tester, and a blank control group was set, wherein the control group was blank (containing no substance). 30 healthy volunteers (students at school) are selected, the age is 20-28 years, and no allergy history (the inclusion and exclusion standards of 2007 cosmetic hygiene standards) exists. After cleaning the back of a subject, the spot tester added with the spot removing cream is pasted on the selected position of the back by using a non-irritating adhesive tape, and the pasted spot tester is lightly pressed by fingers to be uniformly pasted on the skin for 48 hours. The examinee is ordered to keep the spot-pasting part dry within 48h, and violent exercise, scratching the spot-pasting part, long-time sunlight irradiation and the like are avoided. And after 48h, removing the tester and marking, judging under sufficient light after the constant pressure marks disappear after 0min, and performing revisit observation within 72h and 96h respectively.
Table 3 shows the skin adverse reaction grading criteria.
TABLE 3
TABLE 4 adverse skin reaction results
As shown in the experimental data in Table 4, the freckle-removing cream prepared in the examples 5-10 has no serious adverse reaction, and 30 subjects have no positive reaction, and the result judges that the freckle-removing cream prepared in the examples 5-10 has no adverse reaction on human bodies.
Table 4 shows that the umbilical cord extract, the hydrolyzed placenta extract, arbutin, the pomegranate exosomes and the giant knotweed exosomes are reasonably compounded, so that irritation to skin is not easily generated, and the safety of the product is high.
Test example four measurement of color of skin spots
The purpose of the test is as follows: to verify the spot-lightening effect of the spot-removing cream of the present invention, examples 5 to 10 and comparative examples 1 to 5 were tested.
The test method comprises the following steps: the samples of examples 5 to 10, comparative examples 1 to 5 and blank examples were used on the pigmented spots of human faces, and the blank example was carried out without any treatment, and 100 subjects used one sample per 10 subjects and tested for L (lightness) and b (melanin) on the pigmented spots of skin using a CK-PMA6CL400 skin tester, skin moisture using a skin moisture tester CM825, and tested for L, b, skin moisture and skin wrinkles before use and 30 days, respectively, and the measured L value, b value and average value of skin moisture of each sample are shown in Table 5.
Δ L ═ L (after 1 month of the test on the blank or example product) -L (before the start of the test).
Δ b ═ b (before the start of the test) -b (after 1 month of the test on the blank or on the product of the examples).
And subtracting the first detection data from the second detection data of the skin moisture to obtain the change condition of the skin moisture content, wherein the larger the data is, the higher the skin moisture content is.
TABLE 5
| Group/effect value | ΔL | Δb | Change of moisture content |
| Example 5 | 25.69 | 30.75 | 7.07％ |
| Example 6 | 26.12 | 30.96 | 7.09％ |
| Example 7 | 28.73 | 35.01 | 7.16％ |
| Example 8 | 24.59 | 28.71 | 6.55％ |
| Example 9 | 27.89 | 34.54 | 7.01％ |
| Example 10 | 27.92 | 34.67 | 6.99％ |
| Comparative example 1 | 15.89 | 19.21 | 6.02％ |
| Comparative example 2 | 18.46 | 20.37 | 6.35％ |
| Comparative example 3 | 19.85 | 21.13 | 6.47％ |
| Comparative example 4 | 21.81 | 23.89 | 6.53％ |
| Comparative example 5 | 23.42 | 26.36 | 6.86％ |
| Blank group | 3.32 | 4.15 | 2.46％ |
As can be seen from the data in table 5, the spot removing cream prepared in examples 5 to 10 has a good spot lightening effect, reduces melanin more, and whitens the skin with a good effect.
The data show that the pomegranate exosomes and the giant knotweed exosomes are added into the freckle removing cream to achieve the effect of fading freckles, and the pomegranate exosomes and the giant knotweed exosomes are matched with the umbilical cord extract, the hydrolyzed placenta extract and the arbutin according to a specific proportion to achieve a synergistic effect, so that the freckle removing and whitening effects of the freckle removing cream are better.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. A freckle-removing cosmetic composition containing an umbilical cord blood extract is characterized by comprising the following raw materials in parts by weight:
5-15 parts of umbilical cord extract, 1-10 parts of hydrolyzed placenta extract, 0.5-1.5 parts of arbutin, 4-10 parts of pomegranate exosome and 2-6 parts of giant knotweed exosome.
2. The spot-removing cosmetic composition according to claim 1, which comprises the following raw materials in parts by weight:
5-12 parts of umbilical cord extract, 1-8 parts of hydrolyzed placenta extract, 0.5-1.2 parts of arbutin, 4-8 parts of pomegranate exosome and 2-5 parts of giant knotweed exosome.
3. The spot-removing cosmetic composition according to claim 2, which comprises the following raw materials in parts by weight:
8 parts of umbilical cord extract, 5 parts of hydrolyzed placenta extract, 1 part of arbutin, 6 parts of pomegranate exosome and 4 parts of giant knotweed exosome.
4. The spot-removing cosmetic composition according to claim 1, wherein the preparation method of pomegranate exosome or giant knotweed exosome comprises the following steps:
1) grinding pomegranate or giant knotweed rhizome, adding PBS buffer solution, filtering, centrifuging, and taking supernatant;
2) and (4) carrying out ultracentrifugation on the obtained supernatant, taking the precipitate, carrying out heavy suspension on the precipitate by using a buffer solution, and taking the precipitate.
5. The spot-removing cosmetic composition according to claim 4, wherein the rotation speed of the centrifugation in the step 1) is 8000rpm to 9500 rpm; the rotating speed of the centrifugation in the step 1) is 8500rpm-10000 rpm.
6. Use of the spot-removing cosmetic composition according to any one of claims 1 to 5 for preparing water, milk, essence, cream.
7. The freckle-removing cream is characterized by comprising the following components in percentage by weight:
the spot-removing cosmetic composition according to any one of claims 1 to 6, 1 to 3% of a skin conditioner, 5 to 15% of a humectant, 0.2 to 1% of an emulsifier, 0.5 to 1% of an antioxidant, and the balance of water.
8. The spot removing cream of claim 7 wherein the skin conditioning agent is at least one of dipotassium glycyrrhizinate and allantoin; the humectant is hydrogenated polyisobutene; the emulsifier is candelilla wax or cetostearyl alcohol; the antioxidant is p-hydroxyacetophenone.
9. A method of preparing a spot removing cream according to claim 7, comprising the steps of:
s1, mixing the humectant, the emulsifier, the antioxidant and water to obtain a water-phase material;
s2, cooling the water phase material, adding the freckle-removing cosmetic composition and the skin conditioner according to any one of claims 1 to 6, and cooling to obtain the freckle-removing cream.
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| CN103320490A (en) * | 2013-05-13 | 2013-09-25 | 程树军 | Screening method of safety and efficacy of skin antioxidants through use of a plurality of normal human skin cells |
| CN107213084A (en) * | 2017-05-27 | 2017-09-29 | 广州市千邦化妆品有限公司 | A kind of whitening spot-eliminating composition and cosmetics |
| CN110960478A (en) * | 2019-12-04 | 2020-04-07 | 广州博科健康科技集团有限公司 | Freckle-removing cream containing umbilical cord extract and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320490A (en) * | 2013-05-13 | 2013-09-25 | 程树军 | Screening method of safety and efficacy of skin antioxidants through use of a plurality of normal human skin cells |
| CN107213084A (en) * | 2017-05-27 | 2017-09-29 | 广州市千邦化妆品有限公司 | A kind of whitening spot-eliminating composition and cosmetics |
| CN110960478A (en) * | 2019-12-04 | 2020-04-07 | 广州博科健康科技集团有限公司 | Freckle-removing cream containing umbilical cord extract and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115161256A (en) * | 2022-06-20 | 2022-10-11 | 广州远想生物科技股份有限公司 | Giant knotweed suspension cell, exosome thereof, preparation method and application thereof |
| CN115161256B (en) * | 2022-06-20 | 2024-03-12 | 广州远想生物科技股份有限公司 | Polygonum cuspidatum suspension cells, exosomes thereof, and preparation method and application thereof |
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