CN116747151A - Anti-aging composition and preparation method and application thereof - Google Patents
Anti-aging composition and preparation method and application thereof Download PDFInfo
- Publication number
- CN116747151A CN116747151A CN202310855858.5A CN202310855858A CN116747151A CN 116747151 A CN116747151 A CN 116747151A CN 202310855858 A CN202310855858 A CN 202310855858A CN 116747151 A CN116747151 A CN 116747151A
- Authority
- CN
- China
- Prior art keywords
- extract
- aging
- skin
- composition
- composition according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 118
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 81
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 239000000284 extract Substances 0.000 claims abstract description 53
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 43
- 229920001184 polypeptide Polymers 0.000 claims abstract description 36
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 36
- 239000000419 plant extract Substances 0.000 claims abstract description 32
- -1 beta-alanyl hydroxy Chemical group 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- WSGCRSMLXFHGRM-DEVHWETNSA-N (2s)-2-[[(2s)-6-amino-2-[[(2s,3r)-2-[[(2s,3r)-2-[[(2s)-6-amino-2-(hexadecanoylamino)hexanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxybutanoyl]amino]hexanoyl]amino]-3-hydroxypropanoic acid Chemical compound CCCCCCCCCCCCCCCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O WSGCRSMLXFHGRM-DEVHWETNSA-N 0.000 claims abstract description 14
- AJLNZWYOJAWBCR-OOPVGHQCSA-N (4s)-4-acetamido-5-[[(2s)-1-[[(2s)-1-[[(2s)-5-amino-1-[[(2s)-1-[[(2s)-1-amino-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-car Chemical compound OC(=O)CC[C@H](NC(C)=O)C(=C)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(N)=O AJLNZWYOJAWBCR-OOPVGHQCSA-N 0.000 claims abstract description 14
- 229940095094 acetyl hexapeptide-8 Drugs 0.000 claims abstract description 14
- 108010006338 acetyl-glutamyl-glutamyl-methionyl-glutaminyl-arginyl-argininamide Proteins 0.000 claims abstract description 14
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 claims abstract description 8
- 235000008582 Pinus sylvestris Nutrition 0.000 claims abstract description 7
- 239000001839 pinus sylvestris Substances 0.000 claims abstract description 7
- 241000223600 Alternaria Species 0.000 claims abstract 2
- 241000218626 Pinus sylvestris Species 0.000 claims abstract 2
- 239000000306 component Substances 0.000 claims abstract 2
- 238000003756 stirring Methods 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 20
- 238000001914 filtration Methods 0.000 claims description 17
- 238000002156 mixing Methods 0.000 claims description 15
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 9
- 241000206671 Gelidium amansii Species 0.000 claims description 8
- 150000002191 fatty alcohols Chemical class 0.000 claims description 8
- 239000003755 preservative agent Substances 0.000 claims description 8
- 230000002335 preservative effect Effects 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 7
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims 1
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 108010035532 Collagen Proteins 0.000 abstract description 43
- 102000008186 Collagen Human genes 0.000 abstract description 43
- 229920001436 collagen Polymers 0.000 abstract description 43
- 230000009759 skin aging Effects 0.000 abstract description 23
- 230000014509 gene expression Effects 0.000 abstract description 14
- 230000001965 increasing effect Effects 0.000 abstract description 14
- 230000037394 skin elasticity Effects 0.000 abstract description 8
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000003111 delayed effect Effects 0.000 abstract description 3
- 238000009776 industrial production Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 231100000344 non-irritating Toxicity 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 52
- 210000004027 cell Anatomy 0.000 description 40
- 238000012360 testing method Methods 0.000 description 20
- 230000000694 effects Effects 0.000 description 17
- 239000000047 product Substances 0.000 description 11
- 206010051246 Photodermatosis Diseases 0.000 description 10
- 239000002537 cosmetic Substances 0.000 description 10
- 239000000835 fiber Substances 0.000 description 10
- 230000008845 photoaging Effects 0.000 description 10
- 230000002829 reductive effect Effects 0.000 description 10
- 239000004744 fabric Substances 0.000 description 9
- 241001512722 Ecklonia cava Species 0.000 description 8
- 241000206672 Gelidium Species 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 230000008439 repair process Effects 0.000 description 8
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 7
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 7
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 235000019155 vitamin A Nutrition 0.000 description 7
- 239000011719 vitamin A Substances 0.000 description 7
- 229940045997 vitamin a Drugs 0.000 description 7
- 230000032683 aging Effects 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 210000004207 dermis Anatomy 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 230000005855 radiation Effects 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 230000037303 wrinkles Effects 0.000 description 6
- 240000005020 Acaciella glauca Species 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 210000002615 epidermis Anatomy 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 241000113542 Codium tomentosum Species 0.000 description 4
- 241000264279 Sargassum fusiforme Species 0.000 description 4
- 102000004142 Trypsin Human genes 0.000 description 4
- 108090000631 Trypsin Proteins 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000013112 stability test Methods 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 239000012588 trypsin Substances 0.000 description 4
- 229940015975 1,2-hexanediol Drugs 0.000 description 3
- 102000012422 Collagen Type I Human genes 0.000 description 3
- 108010022452 Collagen Type I Proteins 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000195474 Sargassum Species 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 239000000686 essence Substances 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 description 3
- 238000007665 sagging Methods 0.000 description 3
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 3
- ANZUDYZHSVGBRF-UHFFFAOYSA-N 3-ethylnonane-1,2,3-triol Chemical compound CCCCCCC(O)(CC)C(O)CO ANZUDYZHSVGBRF-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 108010014258 Elastin Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001647 drug administration Methods 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 206010033675 panniculitis Diseases 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 229940018958 sargassum fusiforme extract Drugs 0.000 description 2
- 238000013424 sirius red staining Methods 0.000 description 2
- 210000001626 skin fibroblast Anatomy 0.000 description 2
- 230000037393 skin firmness Effects 0.000 description 2
- 210000000434 stratum corneum Anatomy 0.000 description 2
- 210000004304 subcutaneous tissue Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000009056 telangiectasis Diseases 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- NCZPCONIKBICGS-UHFFFAOYSA-N 3-(2-ethylhexoxy)propane-1,2-diol Chemical compound CCCCC(CC)COCC(O)CO NCZPCONIKBICGS-UHFFFAOYSA-N 0.000 description 1
- 241000157280 Aesculus hippocastanum Species 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 244000104272 Bidens pilosa Species 0.000 description 1
- 235000010662 Bidens pilosa Nutrition 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 206010006784 Burning sensation Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
- 108700004714 Gelonium multiflorum GEL Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 208000001126 Keratosis Diseases 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 244000075898 Lantana strigocamara Species 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 235000004347 Perilla Nutrition 0.000 description 1
- 244000124853 Perilla frutescens Species 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010063493 Premature ageing Diseases 0.000 description 1
- 208000032038 Premature aging Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 244000272459 Silybum marianum Species 0.000 description 1
- 235000010841 Silybum marianum Nutrition 0.000 description 1
- 229920002385 Sodium hyaluronate Polymers 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 240000002657 Thymus vulgaris Species 0.000 description 1
- 235000007303 Thymus vulgaris Nutrition 0.000 description 1
- 108010031318 Vitronectin Proteins 0.000 description 1
- 102100035140 Vitronectin Human genes 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 235000014104 aloe vera supplement Nutrition 0.000 description 1
- 231100000360 alopecia Toxicity 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000002469 basement membrane Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 229940059958 centella asiatica extract Drugs 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 229940100524 ethylhexylglycerin Drugs 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 150000002256 galaktoses Chemical class 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036732 histological change Effects 0.000 description 1
- 235000020721 horse chestnut extract Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 125000000468 ketone group Chemical group 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- WXZMFSXDPGVJKK-UHFFFAOYSA-N pentaerythritol Chemical compound OCC(CO)(CO)CO WXZMFSXDPGVJKK-UHFFFAOYSA-N 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000223 polyglycerol Polymers 0.000 description 1
- 210000004896 polypeptide structure Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 230000037390 scarring Effects 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000001585 thymus vulgaris Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9711—Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9717—Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention provides an anti-aging composition, which comprises polypeptide components, plant extracts and water; the polypeptide component comprises beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine, acetyl hexapeptide-8 and palmitoyl pentapeptide-4; the plant extract comprises thallus laminariae extract, cyrtymenia Sparsa extract, pinus sylvestris extract and Alternaria glabra extract. The invention combines the active polypeptide component and the natural plant extract to promote the absorption of the active components in the anti-aging composition for human body and enhance the anti-aging effect; by promoting the generation and expression of collagen, the skin elasticity and compactness are increased, the skin aging is delayed, and the skin aging problem is fundamentally solved. The composition is safe, efficient, green, healthy, good in stability, safe and non-irritating to human bodies, and meets the increasing demands of consumers for treating or caring skin; the preparation method is simple, convenient to operate, easy to meet production conditions and beneficial to industrial production.
Description
Technical Field
The invention relates to the technical field of cosmetics, and in particular relates to an anti-aging composition, a preparation method and application thereof.
Background
The skin is the largest and most important organ of the human body, covering the entire surface of the human body, and is composed of epidermis, dermis and subcutaneous tissue, wherein the epidermis and dermis layers play important roles, such as preventing moisture loss, forming a protective barrier, providing structural support, and the like. However, over time, the expression levels of the epidermis and dermis of the skin change due to factors such as aging, internal and external environments, etc., such as changes in proliferation rate and differentiation of keratinocytes in the epidermis after exposure to ultraviolet radiation for a long period of time, decreased expression of molecules involved in cell adhesion to basal lamina, and decreased skin barrier function; the composition of the extracellular matrix of the dermis layer is also changed in a degenerative way along with the aging process, collagen is greatly lost, the activity of fibroblasts is weakened, the synthesized collagen is gradually reduced, the area of collagen fibers is also reduced, thus leading to loose reticular structure of the dermis layer of the skin, reduced moisture and toughness of skin tissues, and the skin is immediately subjected to the problems of dryness, roughness, wrinkles, slackening, damage and the like, thereby accelerating the skin aging.
In recent years, more and more functional skin care products have been developed to delay skin aging. However, most of the commercially available anti-aging skin care products improve the skin aging state by simply adding exogenous active substances (such as moisturizers, free radical scavengers, sunscreens, etc.), and the actual anti-aging effect is not satisfactory. In addition, the components of the skin care product can only be absorbed by the surface layer of the skin to a great extent, and can not really enter the deep layer of the skin so as to achieve the purposes of permanently updating and supplementing collagen, thus the skin care product can not better play the role of delaying skin aging. Currently, many anti-aging products such as vitamin A, vitronectin and the like are promoted in the market. The glass color has good anti-aging effect, can quickly repair damaged skin cuticle, increase skin elasticity, improve skin fine lines and is popular with people; however, it is expensive and is not suitable for allergic skin people, and allergic reaction is easy to occur in the using process, and uncomfortable symptoms such as skin itch, erythra and the like are caused. The vitamin A has low price, is easy to obtain, has strong antioxidation effect, can resist the generation of skin cell free radicals, reduce the damage of the free radicals to the skin and delay the skin aging; however, the skin care product has great stimulation to the skin, strict requirements on the use times and dosage, and excessive use can easily lead to thinning of skin cuticle and the occurrence of uncomfortable symptoms such as burning sensation, erythema, swelling and the like. Thus, it is found that the above-mentioned compounds do not exhibit an anti-aging effect with a long-lasting, safe and high-efficiency.
However, the polypeptide is expressed almost in cells of a human body as an effective component, and regulates various physiological functions of the human body, and has strong activity, high safety, no side effect on the human body, and has been widely used in cosmetics. Meanwhile, with the progress of society, the development of science and technology and the improvement of living standard, people pursue natural, green, healthy and safe consciousness to be increasingly enhanced, green and functional cosmetics are increasingly popular, natural components are easier to be absorbed by skin, the source is wide, the action mechanism is highly targeted, green and safe, the application of plant extracts in cosmetics is more and more extensive, and the variety of applied plant extracts is more and more.
Chinese patent CN111529427a discloses an active polypeptide compound plant extract anti-aging repair composition and application and essence milk containing the composition, wherein the composition comprises a plant extract and a repair active polypeptide; the repair active polypeptide comprises: 2-8 parts of acetyl hexapeptide-8, 0.5-4 parts of palmitoyl pentapeptide-3 and 0.3-2 parts of pentapeptide-3; the plant extract comprises: 20-200 parts of perilla leaf extract, 18-160 parts of lantana leaf extract and 16-140 parts of sticktight extract, 50-400 parts of European horse chestnut extract, 48-200 parts of thyme extract, 30-360 parts of aloe vera extract, 30-340 parts of centella asiatica extract and 14-120 parts of silybum marianum extract. The polypeptide with the regeneration repair activity is compounded on the basis of the plant extract combination with strong antioxidant activity, so that the skin is repaired, protected and nourished in an omnibearing manner. However, the stability of the composition is to be optimized and the anti-aging effect is limited.
Therefore, it is necessary to combine the active polypeptide with the plant extract to develop an anti-aging composition which is safe, efficient, green, healthy and stable, fundamentally solves the skin aging problem, delays the skin aging, and applies the composition to the technical field of cosmetics so as to meet the increasing demands of consumers for treating or caring skin.
Disclosure of Invention
Aiming at the problems, the invention provides a safe, efficient, green, healthy and stable anti-aging composition, which is prepared by combining active polypeptide components with an anti-aging effect with natural plant extracts, wherein the active polypeptide components and the natural plant extracts act together to promote the absorption of the active components in the anti-aging composition, so that the anti-aging effect of a human body is enhanced; by promoting the generation and expression of collagen, the skin elasticity and compactness are increased, the wrinkles on the skin surface are improved, the skin aging is further delayed, the skin aging problem is fundamentally solved, and the collagen can be used for treating, preventing or repairing skin aging or photoaging.
In a first aspect, the invention provides an anti-aging composition comprising a polypeptide component, a plant extract and water;
the polypeptide component comprises beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine, acetyl hexapeptide-8 and palmitoyl pentapeptide-4;
the plant extract comprises Ecklonia Cava (Ecklonia Cava) extract, cyrtymenia Sparsa (SARGASSUM FUSIFORME) extract, pinus sylvestris (CODIUM TOMENTOSUM) extract, gelidium amansii (Gelidium mcartridge) extract.
The beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine can effectively protect elastin, improve cell activity, repair photodamaged cells, increase skin elasticity, improve compactness and improve roughness. Acetyl hexapeptide-8 is a synthetic peptide consisting of arginine, methionine and acetylated glutamic acid residues. As a high-quality wrinkle-removing component, the composition has excellent wrinkle-removing and anti-aging effects. And palmitoyl pentapeptide-4 has excellent functions of promoting the generation and expression of collagen, increasing the elasticity and the compactness of skin, improving the wrinkles on the surface of the skin, delaying skin aging and playing an anti-aging role.
The inventor finds that when three polypeptide components are used together, the skin tightening effect is excellent, and the skin elasticity can be effectively enhanced. The effect of the three polypeptide components is far better than the effect of the combination of the acetyl hexapeptide-8 and the palmitoyl pentapeptide-4 or the beta-alanyl hydroxyproline diaminobutyric acid benzylamine. The analysis by the inventor is probably due to the fact that the three polypeptide components contain different amino acid polypeptide structures, and the collagen formation promotion under different approaches can be satisfied. However, the content of the beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine cannot be too high, or the solubility of the beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine is easily affected by the generation of a secondary structure, and the polypeptide is easily unstable and decomposed when meeting acid and alkali or metal ions in a solution, for example, the beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine needs to be avoided and is compatible with compounds containing aldehyde groups and ketone groups, metal ions, strong acid, strong alkali and active enzyme substances, or the active ingredients are easily inactivated, and the stability of the composition is easily deteriorated.
Preferably, the anti-aging composition comprises, by mass, 0.001-0.5% of beta-alanyl hydroxyproline diaminobutyric acid benzylamine, 0.001-0.5% of acetyl hexapeptide-8, and 0.001-0.1% of palmitoyl pentapeptide-4; the plant extract comprises Ecklonia Cava (Ecklonia Cava) extract 0.01-3%, cyrtymenia Sparsa (SARGASSUM FUSIFORME) extract 0.01-3%, pinus sylvestris (CODIUM TOMENTOSUM) extract 0.01-2%, and Gelidium (Gelidium marianum) extract 0.01-2%; the balance of water.
The anti-aging composition is compounded by using a plurality of plant extracts, wherein the plant extracts are selected from 4 marine plants, so that the anti-aging composition is strong in vitality and rich in minerals and vitamins; wherein the thallus laminariae extract has antibacterial, antiallergic, antioxidant and antiaging effects; the Sargassum fusiforme extract has antibacterial, antioxidant, antiaging, skin metabolism activating, and skin elasticity improving effects; the extract of Botrytis cinerea has antibacterial, moisturizing and antiaging effects; the gelatine extract has excellent anti-aging effect. Therefore, the three polypeptide components are combined with the 4 plant extracts, and the multiple components are matched to play a role, so that the anti-aging effect is enhanced.
Meanwhile, the inventors have unexpectedly found that when three polypeptide components are used in combination with the plant extract of 4, the stability of the composition can be enhanced, the dosage of the polypeptide components can be increased, the decomposition of the polypeptide components can be reduced, and the skin effect of the composition can be further enhanced. The analysis by the inventors is probably due to the fact that the components of various plant extracts contain components such as polysaccharides, saponins, vitamins, phenols and the like with different structures, and can act together with polypeptide components to promote the osmotic absorption of active components. The Gelidium amansii (Gelidium amansicum) extract contains a large amount of calcium, potassium, sodium and ammonium of polysaccharide sulfate consisting of galactose and dehydrated galactose, has a certain viscosity increasing effect, can improve the viscosity of the composition, enhances the stable dispersion of polypeptide components and small molecular polysaccharide components, and avoids the problems of decomposition, inactivation or aggregation and the like of the composition. However, excessive use of Gelidium amansii (Gelidium amansii) extract can result in a sticky feel during use.
Further preferably, the polypeptide component comprises, by mass, 0.01-0.2% of beta-alanyl hydroxyproline diaminobutyric acid benzylamine, 0.01-0.2% of acetyl hexapeptide-8, 0.005-0.05% of palmitoyl pentapeptide-4;
the plant extract comprises 0.1-1wt% of Ecklonia Cava (Ecklonia Cava) extract, 0.1-1wt% of Cyrtymenia Sparsa (SARGASSUM FUSIFORME) extract, 0.1-1wt% of Pinus sylvestris (CODIUM TOMENTOSUM) extract, and 0.1-1wt% of Gelidium (Gelidium marianum) extract.
Further preferably, the polypeptide component comprises, by mass, 0.05 to 0.1% of beta-alanyl hydroxyproline diaminobutyric acid benzylamine, 0.05 to 0.1% of acetyl hexapeptide-8, 0.01 to 0.03% of palmitoyl pentapeptide-4;
the plant extract comprises 0.2-0.5% of Ecklonia Cava (Ecklonia Cava) extract, 0.5-0.8% of Cyrtymenia Sparsa (SARGASSUM FUSIFORME) extract, 0.5-0.8% of Pinus sylvestris (CODIUM TOMENTOSUM) extract, and 0.2-0.5% of Gelidium (Gelidium marianum) extract.
Preferably, the anti-aging composition further comprises fatty alcohol, preservative and solubilizer.
Further preferably, the anti-aging composition further comprises, in weight percent, 3-10% of fatty alcohol, 1-10% of preservative and 0.1-2% of solubilizer.
Preferably, the fatty alcohol is one or more of butanediol, propylene glycol, isopropanol or polyethylene glycol.
Further preferably, the preservative is one or more of ethylene glycol, glycerol, pentaerythritol, 1, 2-pentanediol, 1, 2-hexanediol and ethylhexyl glycerol.
Further preferably, the preservative is one or more of 1, 2-pentanediol, 1, 2-hexanediol, and ethylhexyl glycerin.
Preferably, the solubilizer is one or two of PPG-13-decyl tetradecyl polyether-24 or polyglycerin fatty acid ester.
In a second aspect, the invention provides a method of preparing an anti-ageing composition comprising the steps of:
(1) Placing the polypeptide component, fatty alcohol and solubilizer in a mixing container, adding water at a first designated temperature, stirring uniformly, and filtering to obtain a first mixture;
(2) Placing the plant extract and the preservative in a mixing container, adding water at a second designated temperature, uniformly stirring, and filtering to obtain a second mixture;
(3) And uniformly mixing the first mixture and the second mixture, stirring, and filtering to obtain the anti-aging composition.
In order to further enhance the stability of the anti-aging composition, the inventors have found that the uniform dispersion of the polypeptide component and the plant extract component in the system can be effectively enhanced by dissolving the polypeptide component into a mixture of a specific mesh number and then adding the component such as the plant extract. Avoiding the problems of agglomeration of polypeptide or micromolecular polysaccharide, precipitation, influence on the stability and the use effect of the composition and the like caused by uneven dispersion.
Preferably, in the steps (1) to (3), the mesh number of the filtration is 200 to 500 mesh.
Further preferably, in the steps (1) to (3), the mesh number of the filtration is one of 200 mesh, 400 mesh and 500 mesh.
Preferably, the first specified temperature in step (1) is 15-40 ℃.
More preferably, the first predetermined temperature in the step (1) is one of 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃ and 40 ℃.
Preferably, the second specified temperature in step (2) is 15-45 ℃.
More preferably, the second predetermined temperature in the step (2) is one of 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃,40 ℃, 45 ℃.
Preferably, the stirring speed in the step (3) is 40-50r/min, and the stirring time is 5-15min.
Further preferably, the stirring speed in the step (3) is one of 40r/min, 45r/min and 50r/min, and the stirring time is one of 5min, 10min and 15min.
In a third aspect, the invention provides the use of an anti-ageing composition in a skin care product.
The anti-aging composition is applied to the technical field of cosmetics, so that the cosmetics have the anti-aging function, can increase the elasticity and compactness of skin, improve wrinkles on the surface of the skin, treat, prevent or repair skin aging or photoaging, delay skin aging, and have simple and convenient use method, and can meet the increasing demands of consumers for treating or caring the skin, thereby better improving the economic value of the anti-aging composition.
Preferably, the use in skin care products includes use in lotions, essences, creams, ointments or masks.
Preferably, the anti-aging composition is added to the skin care product in an amount of 0.5-10wt%.
Further preferably, the anti-aging composition is added to the skin care product in an amount of preferably 0.5 to 10wt%, 1 to 8wt% or 2 to 5wt%. The anti-aging composition can exert better effect by controlling the addition amount of the anti-aging composition, so that the skin care product added with the anti-aging composition has better anti-aging effect.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention combines the active polypeptide component with the anti-aging effect and the plant extract to act synergistically, and promotes the absorption of the active components in the anti-aging composition for human body, thereby enhancing the anti-aging effect; the anti-aging composition is safe, efficient, green, healthy, good in stability, safe and non-irritating to human bodies, can be applied to the field of cosmetics, and meets the increasing demands of consumers for treating or caring skin.
2. The composition disclosed by the invention can promote the generation and expression of collagen, increase the elasticity and compactness of skin, and improve the wrinkles on the surface of the skin, so that the skin aging is delayed, the problem of skin aging can be fundamentally solved, and the composition can be used for treating, preventing or repairing skin aging or photoaging.
3. The preparation method of the composition provided by the invention is simple, convenient to operate, good in stability and easy to meet production conditions, and is beneficial to industrial production.
Drawings
FIG. 1 is a graph showing the effect of the test sample in example 1 on the collagen content;
FIG. 2 is a graph showing the results of the experiment of collagen fibers of HSF cells observed under a 100-fold mirror in example 1;
Detailed Description
In order to facilitate understanding of the invention, the meaning of some terms and expressions used in the present invention is described as follows:
in the present invention, the term "skin" is understood to mean the layers that make up it, from the uppermost or stratum corneum to the lowermost or subcutaneous tissue, both endpoints being included. These layers are composed of different types of cells, such as keratinocytes, fibroblasts, melanocytes, and/or adipocytes, among others. In the present invention, the term "skin" includes the scalp.
The term "treatment" refers to administration of a peptide compound according to the invention to reduce or eliminate a disease or condition, or to reduce or eliminate one or more symptoms associated with such a disease or condition. The term "treating" also encompasses the ability to reduce or eliminate the physiological consequences of the disease or disorder.
The term "preventing" refers to the ability of a peptide compound of the invention to prevent, delay, or hinder the appearance or progression of a disease or disorder prior to its appearance.
The term "repair" refers to the ability of a peptide compound of the invention to improve, alleviate or restore its original shape after the appearance of a disease or condition.
The term "aging" refers to changes experienced by skin with age (natural aging), or by exposure to sunlight (photoaging) or to environmental pollutants such as chemical dirt or pollutants, tobacco smoke, etc., and includes all externally visible and/or perceivable changes by touch, such as, and not limited to: the development of discontinuities in the skin (e.g., wrinkles, fine lines, expression lines, stretch lines, streaks, grooves, irregularities or roughness, increased pore size, loss of moisture, loss of elasticity, loss of firmness, loss of smoothness, loss of deformability, loss of resilience), sagging skin (e.g., sagging cheek, under-eye appearance of bags, or appearance of double chin, etc.), changes in skin color (e.g., scarring, redness, bags, or appearance of hyperpigmented areas such as age spots or freckles, etc.), abnormal differentiation, hyperkeratinization, elastosis, keratosis, alopecia, orange-peel-like skin, loss of collagen structure, and other histological changes in the stratum corneum, dermis, epidermis, vascular system (e.g., appearance of spider veins or telangiectasia), or those tissues adjacent to the skin.
The term "photoaging" refers to premature aging of the skin due to prolonged exposure of the skin to ultraviolet radiation, which exhibits the same physiological characteristics as natural aging, for example and without limitation: relaxation, sagging, color changes or pigmentation irregularities, abnormalities and/or hyper-keratinization.
For a better understanding of the present invention, reference will now be made in detail to the present invention, examples of which are illustrated in the accompanying drawings, however, it being understood that the examples, test examples and the accompanying drawings are for illustrative purposes only and are not intended to limit the scope of the present invention.
Examples
Example 1
The first aspect of the present embodiment provides an anti-aging composition, wherein the formulation of the composition comprises the following components in percentage by mass:
beta-alanyl hydroxyproline diaminobutyric acid benzylamine is purchased from Shenzhen Viqi; acetyl hexapeptide-8 is purchased from Shenzhen Viqi; palmitoyl pentapeptide-4 is purchased from Shenzhen Viqi; PPG-13-decyl tetradecyl polyether-24, available from Shenzhen Viqi; the thallus laminariae extract is purchased from Fangzhou Baifubo; the sargassum fusiforme extract is purchased from Guangzhou Baifubo; the extract of Pinus sylvestris is purchased from Fangzhou Baifubo; the gelonin herb extract was purchased from Guangzhou Baifubo.
The second aspect of the present embodiment provides a method for preparing an anti-aging composition, which specifically comprises the following steps:
(1) Weighing the formula amount of beta-alanyl hydroxyproline diaminobutyric acid benzylamine, acetyl hexapeptide-8, palmitoyl pentapeptide-4, PPG-13-decyl tetradecyl polyether-24 and butanediol, placing the materials in a mixing container, adding 40 ℃ water, stirring uniformly, and filtering with 400-mesh filter cloth to obtain a first mixture;
(2) Weighing thallus laminariae extract, cyrtymenia Sparsa extract, sargassum Bolus extract, gelidium amansii extract, 1,2 pentanediol, and 1,2 hexanediol, placing in a mixing container, adding 30deg.C water, stirring, and filtering with 400 mesh filter cloth to obtain second mixture;
(3) Mixing the first mixture and the second mixture uniformly, stirring for 10min at a rotation speed of 40r/min to form a uniform solution, and filtering with 200 mesh filter cloth to obtain the antiaging composition.
Example 2
The first aspect of the present embodiment provides an anti-aging composition, wherein the formulation of the composition comprises the following components in percentage by mass:
in a second aspect, this example provides a method for preparing an anti-aging composition, the steps being as described in example 1.
Example 3
The first aspect of the present embodiment provides an anti-aging composition, wherein the formulation of the composition comprises the following components in percentage by mass:
in a second aspect, this example provides a method for preparing an anti-aging composition, the steps being as described in example 1.
Example 4
The first aspect of the present embodiment provides an anti-aging composition, wherein the formulation of the composition comprises the following components in percentage by mass:
the second aspect of the present embodiment provides a method for preparing an anti-aging composition, which specifically comprises the following steps:
(1) Weighing the formula amount of beta-alanyl hydroxyproline diaminobutyric acid benzylamine, acetyl hexapeptide-8, palmitoyl pentapeptide-4, PPG-13-decyl tetradecyl polyether-24 and propylene glycol, placing the materials in a mixing container, adding 15 ℃ water, stirring uniformly, and filtering with 400-mesh filter cloth to obtain a first mixture;
(2) Weighing thallus laminariae extract, cyrtymenia Sparsa extract, sargassum Bolus extract, gelidium amansii extract, and 1,2 pentanediol, placing in a mixing container, adding 45deg.C water, stirring, and filtering with 500 mesh filter cloth to obtain second mixture;
(3) Mixing the first mixture and the second mixture uniformly, stirring for 15min at a rotation speed of 50r/min to form a uniform solution, and filtering with 200 mesh filter cloth to obtain the antiaging composition.
Example 5
The first aspect of the present embodiment provides an anti-aging composition, wherein the formulation of the composition comprises the following components in percentage by mass:
the second aspect of the present embodiment provides a method for preparing an anti-aging composition, which specifically comprises the following steps:
(1) Weighing the formula amount of beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine, acetyl hexapeptide-8, palmitoyl pentapeptide-4, polyglycerol fatty acid ester and isopropanol, placing in a mixing container, adding 25 ℃ water, stirring uniformly, and filtering with 200-mesh filter cloth to obtain a first mixture;
(2) Weighing thallus laminariae extract, cyrtymenia Sparsa extract, sargassum extract, gelidium amansii extract, polyalcohol, and ethylhexyl glycerol, placing in a mixing container, adding 15 deg.C water, stirring, and filtering with 200 mesh filter cloth to obtain a second mixture;
(3) Mixing the first mixture and the second mixture uniformly, stirring for 5min at a rotation speed of 45r/min to form a uniform solution, and filtering with 200 mesh filter cloth to obtain the antiaging composition.
Example 6
Preparation of essence containing anti-aging composition
The preparation method comprises the following steps: stirring and heating the purified water to 85 ℃, and preserving heat for 30min; pre-dissolving sodium hyaluronate and xanthan gum in butanediol, adding into water, stirring and dissolving completely; stirring and cooling to 35 ℃, adding the rest ingredients, and stirring uniformly.
Performance testing
1. Collagen content test
1.1 reagents and materials
Fetal bovine serum, DMEM medium, phosphate buffer, trypsin, collagen I ELISA kit, BCA protein kit.
1.2 instruments
An enzyme-labeled instrument, a CO2 incubator and an ultra-clean workbench.
1.3 cell lines
Human Skin Fibroblasts (HSF) were purchased from the national academy of sciences typical culture collection committee Shanghai cell bank.
1.4 sample to be tested
Drug administration group: vitamin A, vitriol, and the composition, the test concentrations of the samples are 40ppm and 80ppm.
Blank control group: PBS.
UV group: UV radiation, PBS was added.
1.5 Experimental methods
Taking HSF cells in logarithmic growth phase, adding 0.25% trypsin digestion solution, digesting to enable adherent cells to fall off, counting 1-4×106 cells/mL, and preparing cell suspension;
the cells were properly diluted and inoculated into 6-well plates at 105/well, and a UV photoaging model was established when the cells were grown to about 80%. 200. Mu.L of PBS was added to the blank, medium was supplemented to 800. Mu.L, and UV irradiation was not performed; the UV group and the administration group were repeatedly washed to colorless by adding an appropriate amount of PBS, adding 200. Mu.L of PBS, and irradiating under an 80mJ/cm2UV lamp with a lamp-to-flask spacing of 15cm. After irradiation, PBS was discarded, the UV group was added to the PBS solution and medium to 800. Mu.L, and the dosing group was added to the medium and the drug was diluted to 800. Mu.L. The blank, UV, and dosing groups continued at 37deg.C, 5% CO 2 Incubate in incubator for 48h.
After the end of the culture, the 1 st well cells were digested and counted and diluted to 0.5X10 6 After cells in the remaining wells were scraped off with cell scrapes and resuspended in 500. Mu.L, 50. Mu.L of ultrasound was applied to all wells for 30s and the total protein was measured by BCA, and the other wells were diluted to a concentration of 0.5X10 s according to the 1 st well protein concentration 6 And each mL. Ultrasonically crushing the cell suspension with the concentration regulated for 30s, centrifuging at 1500xg for 15min, and collecting fine powderThe cell supernatants were sampled and operated according to the collagen I ELISA protocol. The OD of each well was measured sequentially with an microplate reader at 450nm over 15min.
1.6 experimental results
Collagen is the most abundant protein found in connective tissue, and collagenase is synthesized and secreted by fibroblasts, and can degrade collagen in skin to cause skin aging. Therefore, the method can inhibit the expression of collagenase in cells, and improve the content of collagen, and has important effects on preventing aging and increasing skin plumpness and compactness. In the ultraviolet overexposure environment, collagenase and elastase activities are greatly increased, elastin hydrolysis, and collagen synthesis is also inhibited. The experiment adopts a test sample to treat cells after ultraviolet radiation, and detects the content of collagen I in corresponding cells so as to determine whether the composition can promote the generation of collagen.
2. Collagen fiber experiment
2.1 reagents and materials
Fetal bovine serum, DMEM medium, phosphate buffer, trypsin, biological sirius red staining kit.
2.2 instruments
Optical microscope, CO2 incubator, ultra clean bench.
2.3 cell lines
Human Skin Fibroblasts (HSF) were purchased from the national academy of sciences typical culture collection committee Shanghai cell bank.
2.4 sample to be tested
Drug administration group: vitamin A, vitriol, and the composition, the test concentrations of the samples are 40ppm and 80ppm.
Blank control group: PBS.
UV group: UV radiation, PBS was added.
2.5 Experimental methods
Taking HSF cells in logarithmic growth phase, adding 0.25% trypsin digestion solution, digesting to enable adherent cells to fall off, counting 1-4×106 cells/mL, and preparing cell suspension;
the cells were properly diluted and inoculated into 6-well plates at 105/well, and a UV photoaging model was established when the cells were grown to about 80%. 200. Mu.L of PBS was added to the blank, medium was supplemented to 800. Mu.L, and UV irradiation was not performed; the UV group and the administration group were repeatedly washed to colorless by adding an appropriate amount of PBS, adding 200. Mu.L of PBS, and irradiating under an 80mJ/cm2UV lamp with a lamp-to-flask spacing of 15cm. After irradiation, PBS was discarded, the UV group was added to the PBS solution and medium to 800. Mu.L, and the dosing group was added to the medium and the drug was diluted to 800. Mu.L. The blank, UV, and dosing groups were incubated in a 5% CO2 incubator at 37℃for a further 48h.
After the completion of the culture, the cells were fixed with 4% paraformaldehyde for 15min, stained according to the instructions of the sirius red staining kit, and observed under an optical microscope.
2.6 experimental results
The experiment adopts a test sample to treat cells after ultraviolet radiation, detects the density of corresponding cells and the area of collagen fibers in the same unit, and qualitatively analyzes the collagen fibers in the cells to determine whether the composition can promote the expression of collagen.
3. Stability test (determination of stability of polypeptide Components in compositions)
Test conditions: the samples were packed in white polyethylene plastic bottles and placed in a 40℃constant temperature and humidity cabinet, at room temperature (25 ℃) and refrigerated (4-8 ℃) respectively.
Sampling time: samples were taken for measurement at 0, 1,2, 3, and 6 months, respectively.
Inspection item: properties, pH and content
The inspection method comprises the following steps:
the test is based on: the four general rules 9001 of the Chinese pharmacopoeia 2020 edition are guidelines for testing the stability of the raw materials and the preparation.
New Dab is the abbreviation of beta-alanyl hydroxyproline diaminobutyric acid benzylamine.
"YvesXPeP" refers to the designation of the composition of beta-alanyl hydroxypropionyl diaminobutyrate benzylamine, acetyl hexapeptide-8, palmitoyl pentapeptide-4 in each example.
Results of Performance test
Samples of the anti-aging composition of example 1 were subjected to a collagen content test, a collagen fiber test, and a stability test, and the results of the tests are shown in fig. 1, fig. 2, table 1, table 2, and table 3.
1. The effect of the test sample on collagen content is shown in fig. 1.
The results showed that the collagen content of the UV group was greatly reduced compared to the blank group, indicating that the photoaging model was successfully established. Compared with the UV group, the vitamin A in the range of 40-80ppm and 40ppm of the vitriol cause can not increase the content of the collagen in cells after ultraviolet irradiation, the cell shows obvious cytotoxicity, and 80ppm of the vitriol cause can increase the content of the collagen; the composition of the invention can obviously improve the content of collagen at a low concentration of 40ppm and can also obviously improve the content of collagen at a low concentration of 80ppm, promote the expression of collagen and have excellent function of promoting the generation of collagen, which shows that the composition of the invention has better technical effects than vitamin A and vitriol.
From the results, the composition of the invention can increase the collagen content in cells after ultraviolet irradiation, promote collagen generation, thereby increasing skin elasticity and/or skin firmness, and delay skin aging, and can be used for preventing and even treating skin relaxation, and treating, preventing or repairing skin aging or photoaging. Meanwhile, the invention also discloses the combined use of the active polypeptide component and the plant extract, which is safe, efficient, green, healthy, good in stability, free from toxic effect on cells, capable of enhancing the anti-aging effect and applicable to the field of cosmetics.
2. The collagen fiber test can reflect the collagen expression capacity of cells, and the test result is shown in fig. 2.
The cell density of the UV group is reduced compared with that of the blank control group, and the collagen fiber area in the same unit is also reduced; compared with the UV group, the cell density and the collagen fiber area of the vitamin A group are reduced, obvious cytotoxicity is shown, and the vitreous color factor in the range of 40-80ppm and the composition disclosed by the invention can improve the cell density, increase the collagen fiber area in the same unit, promote the collagen expression, improve the symptom of reduced collagen content caused by UV irradiation of cells, and are dose-dependent. Wherein, the composition of the invention has better technical effect than the glass color factor.
From the above, it can be seen that the composition of the present invention can promote collagen expression, thereby increasing skin elasticity and/or skin firmness, and delaying skin aging, and can be used for preventing or even treating skin relaxation, and treating, preventing or repairing skin aging or photoaging. Meanwhile, the invention also discloses the combined use of the active polypeptide component and the plant extract, which is safe, efficient, green, healthy, good in stability, free from toxic effect on cells, capable of enhancing the anti-aging effect and applicable to the field of cosmetics.
3. Stability test results
TABLE 1 accelerated (40 ℃ C.) test results
TABLE 2 results of Normal temperature (25 ℃ C.) test
TABLE 3 test results of refrigeration (4-8deg.C)
From a combination of tables 1-3, it can be seen that the anti-aging composition of the present invention has no significant change in solution properties and pH under the conditions of 40℃acceleration, normal temperature and refrigeration. The total peptide content is reduced with time under the three conditions, but the reduction amplitude is smaller, the total peptide content is below 10%, and the solution content is relatively stable.
The stability test was performed for example 1, and the pH and total peptide results were measured as shown in tables 4 and 5 below.
TABLE 4 Table 4
TABLE 5
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiment and all such alterations and modifications as fall within the scope of the embodiments of the invention.
Finally, it is further noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article or terminal device comprising the element.
The above detailed description of an anti-aging composition, its preparation method and application provided by the present invention, the specific examples are applied herein to illustrate the principles and embodiments of the present invention, the above examples are only used to help understand the method and core idea of the present invention; meanwhile, as those skilled in the art will have variations in the specific embodiments and application scope in accordance with the ideas of the present invention, the present description should not be construed as limiting the present invention in view of the above.
Claims (10)
1. An anti-aging composition, characterized in that the raw materials comprise polypeptide components, plant extracts and water; the polypeptide component comprises beta-alanyl hydroxy prolyl diaminobutyric acid benzylamine, acetyl hexapeptide-8 and palmitoyl pentapeptide-4; the plant extract comprises thallus laminariae extract, cyrtymenia Sparsa extract, pinus sylvestris extract, and Alternaria glabra extract.
2. An anti-aging composition according to claim 1, wherein the anti-aging composition comprises, in mass percent, β -alanyl hydroxyproline diaminobutyric acid benzylamine 0.001-0.5%, acetyl hexapeptide-8.001-0.5%, palmitoyl pentapeptide-4.001-0.1%;
the plant extract comprises 0.01-3% of thallus laminariae extract, 0.01-3% of Cyrtymenia Sparsa extract, 0.01-2% of Phaeophyllum pinnatifida extract, and 0.01-2% of Gelidium amansii extract; the balance of water.
3. An anti-ageing composition according to claim 1, wherein the anti-ageing composition further comprises a fatty alcohol, a preservative, a solubiliser.
4. An anti-ageing composition according to claim 3, wherein the anti-ageing composition further comprises, in weight percent, 3-10% fatty alcohol, 1-10% preservative and 0.1-2% solubiliser.
5. An anti-ageing composition according to claim 4, wherein the fatty alcohol is one or more of butylene glycol, propylene glycol, isopropanol or polyethylene glycol.
6. A method of preparing an anti-ageing composition according to any of claims 1 to 5, comprising the steps of:
(1) Placing the polypeptide component, fatty alcohol and solubilizer in a mixing container, adding water at a first designated temperature, stirring uniformly, and filtering to obtain a first mixture;
(2) Placing the plant extract and the preservative in a mixing container, adding water at a second designated temperature, uniformly stirring, and filtering to obtain a second mixture;
(3) And uniformly mixing the first mixture and the second mixture, stirring, and filtering to obtain the anti-aging composition.
7. The method of claim 6, wherein the first specified temperature in step (1) is 15-40 ℃.
8. The method of preparing an anti-aging composition according to claim 6, wherein the second prescribed temperature in step (2) is 15 to 45 ℃.
9. Use of an anti-ageing composition according to any of claims 1-5 in a skin care product.
10. Use of an anti-ageing composition according to claim 9 in a skin care product, wherein the anti-ageing composition is added to the skin care product in an amount of 0.5-10wt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310855858.5A CN116747151A (en) | 2023-07-12 | 2023-07-12 | Anti-aging composition and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310855858.5A CN116747151A (en) | 2023-07-12 | 2023-07-12 | Anti-aging composition and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116747151A true CN116747151A (en) | 2023-09-15 |
Family
ID=87953260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310855858.5A Pending CN116747151A (en) | 2023-07-12 | 2023-07-12 | Anti-aging composition and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116747151A (en) |
-
2023
- 2023-07-12 CN CN202310855858.5A patent/CN116747151A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105125476B (en) | A kind of anti-wrinkle essence and preparation method thereof | |
| JP5010775B2 (en) | Use of ellagic acid and its derivatives in cosmetics and dermatology | |
| JPH10509735A (en) | Use of vitamin C or a derivative thereof or an analog thereof for promoting skin elastin synthesis | |
| JP2001522791A (en) | Use of D-xylose, its esters, and xylose-containing oligosaccharides for improving the functionality of epidermal cells. | |
| JP2006273857A (en) | Plant-based formulation for improving moisture retention, texture and appearance of skin | |
| CN105663020A (en) | Spraying water with long-acting moisture-preserving capacity and preparation method thereof | |
| CN109350579B (en) | Whitening cosmetic additive, whitening skin-refreshing lotion and preparation method thereof | |
| CN110833516B (en) | Polypeptide composition with moisturizing effect | |
| CN112043619B (en) | Peptide composition with repairing effect and preparation method and application thereof | |
| CN104887542B (en) | A cosmetic composition | |
| JP2018538256A (en) | PALMARIA Synergistic extract of PALMATA and jasmine, compositions containing it and their use | |
| KR20150120426A (en) | Cosmetic composition containing a brown alga extract, a yeast extract and ascorbic acid | |
| CN112137951A (en) | Silicone-oil-free transparent amino acid shampoo capable of strengthening hair and repairing scalp barrier and preparation method thereof | |
| CN112842985A (en) | Moisturizing and repairing composition | |
| CN110721148A (en) | Multidirectional pulling and tightening polypeptide essence and preparation method thereof | |
| CN107365355B (en) | Anti-wrinkle peptide and cosmetic comprising same | |
| CN114712275A (en) | Anti-wrinkle repair composition and preparation method thereof, and cosmetic and preparation method thereof | |
| CN105749333B (en) | A kind of hyaluronic acid medical dressing and preparation method thereof | |
| CN110368347A (en) | Anti-ageing reparation double-layer bi-color essence of one kind and preparation method thereof | |
| KR102606356B1 (en) | Cosmetic composition for regenerating skin and improving skin wrinkle | |
| KR20050110720A (en) | Cosmetic composition for moisturizing effect on the skin | |
| CN116270392B (en) | Composition with moisturizing effect and application thereof | |
| CN111568781A (en) | Skin care gel and preparation method thereof | |
| KR20140145274A (en) | Composition for skin moisturizing containing oxygen dissolving water with a polymer composition | |
| CN110025523A (en) | A kind of moisturizing maintenance composition of the leaf juice containing aloe and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |