CN1167331C - Method for breeding light- and temp.-sensitive nucleus sterile line rice with plant age regulatory fertility - Google Patents
Method for breeding light- and temp.-sensitive nucleus sterile line rice with plant age regulatory fertility Download PDFInfo
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- CN1167331C CN1167331C CNB021151539A CN02115153A CN1167331C CN 1167331 C CN1167331 C CN 1167331C CN B021151539 A CNB021151539 A CN B021151539A CN 02115153 A CN02115153 A CN 02115153A CN 1167331 C CN1167331 C CN 1167331C
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Abstract
The present invention relates to a method for breeding a rice light-temperature sensitive nuclear sterile line with plant age regulatory fertility, which belongs to the field of rice breeding. A rice variety (line) containing plant age regulatory fertility genes is selected to mutually hybridize with the light-temperature sensitive nuclear sterile line, and more plant age regulatory fertility genes are integrally aggregated with light-temperature sensitive nuclear sterile genes by selection to breed a light-temperature sensitive nuclear sterile line with obvious plant age regulatory fertility. The seedling plants of the sterile line has lower sterile light-temperature critical value and longer sterile period and can be used for local safe breeding; simultaneously, because of having higher sterile light-temperature critical value, old-plant age regenerating plants can be normally propagated in local so as to better solve the contradiction between the breeding and the propagation of practical sterile lines, and moreover, the condition limitation of allopatric propagation or cold-penetration propagation is relieved, manpower and material resources are saved, and the safety for utilizing the sterile lines is enhanced. Simultaneously, because in-situ propagation can be carried out for generation addition in the process of breeding, a breeding process is accelerated.
Description
Technical field
The invention belongs to rice breeding technology, particularly the seed selection of rice photo-thermo-sensitive genic male sterile line and rice heterosis utilization.
Technical background
At present, three line method and two is arranged is two kinds of approach of method to rice heterosis utilization.China's three line method utilizes rice heterosis, as far back as last century the seventies just large-area applications in production, it is to utilize cytoplasmic-nuclear male sterility to realize three series mating and by male sterile line and maintenance line outbreeding male-sterile seed, male sterile line with recover system's hybridization production first-filial generation seed and supply production application.Two is that to utilize rice heterosis also be China initiative to method, last century, the nineties began large-area applications on producing, it is to examine sterile new germ plasm by the rice photo-thermo-sensitive that utilizes China's early seventies in last century to find to cultivate photo-thermo sensitive male nuclear sterile rice line, this male sterile line shows the sterile production of hybrid seeds that is used under long light hot environment, performance can be educated and is used for numerous kind under short light low temperature, one is dual-purpose, thereby has simplified the seed production link, has reduced cost; The fertility of photo-thermo-sensitive genic male sterile line is controlled by recessive homozygous gene in addition, normal kind all can make to recover system and its combo, the restriction that is not recovered gene, thereby more than three line method recovery system, combo is free relatively, also is easier to utilize inter-subspecific heterosis by importing wide compatibility gene.Yet the fertility of photo-thermo-sensitive genic male sterile line changes and to be subjected to the particularly influence of temperature condition of light and temperature condition, thereby the safe production of hybrid seeds of male sterile line is a pair of contradiction with good numerous kind, and the sterile light temperature of male sterile line critical value is low, the production of hybrid seeds safe numerous kind difficult.Produce at present the low thermo-sensitive type male sterile line of going up seed selection, because the sterile temporary dividing value is low, thereby the sterile phase is long, and the production of hybrid seeds is safer, but is difficult to numerous kind under field conditions (factors), needs numerous kind of the method for employing sensitive periods cold water string irrigating.This has increased the seed production cost on the one hand, also is subjected to the restriction of cold water resource on the other hand.In addition aspect the seed selection of low thermo-sensitive type male sterile line because this class breeding material can not change under local condition and can educate, thereby breeding material add generation and seed selection is subjected to conditionality, thereby influence breeding scale and progress, hindered breeding process.
Summary of the invention
Purpose one of the present invention is by selecting strain has the low critical light temperature value of obvious regulating and controlling effect age to fertility photo-thermo sensitive male nuclear sterile rice line, utilize the low strain of growing directly from seeds of sterile light temperature critical value to carry out the safe production of hybrid seeds at big ecological space and ecological period, and the characteristics of utilizing old strain regeneration plant in age sterile temporary dividing value to raise greatly can be carried out numerous kind in the same ecological space and the period of the strain safety production of hybrid seeds of growing directly from seeds, thereby the local production of hybrid seeds and numerous kind of contradiction of can not and deposit have been solved, the trouble of having removed relocation propagation from or having needed the cold-penetration condition to breed is for numerous kind of practical photo-thermo-sensitive genic male sterile line finds a new way that cost is low and effective.The 2nd, can be gathered in strain in the new photo-thermo-sensitive genic male sterile line to the fertility controlling gene age by hybridization and selection, and can the sterile light temperature critical value breeding goal of new sterile line is lowlyer fixed, make the new sterile line production of hybrid seeds safer, practicality is stronger, in seed selection adds for process, can utilize simultaneously old strain regeneration strain in age on the spot numerous kind add generation, make seed selection work can make full use of local advantage and accelerate breeding process.
The present invention comes from us and discovers some rice varieties, is to have strain regulatory fertility in age gene with photo-thermo sensitive male nuclear sterile rice line, and its fertility of photo-thermo sensitive male nuclear sterile rice line with these genes changes except that being subjected to the light and temperature condition regulation and control, also is subjected to the strain influence in age.The strain older sterile light temperature of regeneration strain critical value ratio in the age plant height that grows directly from seeds, breed the strain of growing directly from seeds that grows up to behind the planting seed of being received on the regeneration strain and be returned to original low sterile light temperature critical value again, the highly stable heredity of this characteristic energy, and discover that this characteristic is by controlled by multiple genes.
The present invention is achieved in that at first screening has the photo-thermo sensitive male nuclear sterile rice line of strain regulatory fertility in age gene, whether variant by the strain of under same ecotope, relatively growing directly from seeds with the sterile light temperature critical value of old strain regeneration strain in age, if the sterile light temperature critical value of old strain regeneration strain in age, determines then that this photo-thermo sensitive male nuclear sterile rice line has strain regulatory fertility in age gene obviously than the plant height that grows directly from seeds; Again from the rice varieties of different genotype, be with photo-thermo-sensitive genic male sterile line, by with rice varieties, be and do not have the photo-thermo sensitive male nuclear sterile rice line hybridization of strain regulatory fertility in age gene, again from filial generation F
2Select the photoperiod-temperature sensitive genie male-sterile line strain to differentiate whether have strain regulatory fertility in age characteristic in the colony, as have this characteristic, determine that then this rice varieties, cording have strain regulatory fertility in age gene, filter out the different germplasms with strain regulatory fertility in age gene; Select for use thereafter through screening have the germplasm of strain regulatory fertility in age gene and different genotype rice varieties, be or photo-thermo-sensitive genic male sterile line mutual cross mutually that cross method comprises: (1) is selected rice varieties with strain regulatory fertility in age gene for use, is to hybridize with photo-thermo-sensitive genic male sterile line; (2) with photo-thermo sensitive male nuclear sterile rice line with have strain regulatory fertility in age gene rice varieties, be hybridization, then with have strain regulatory fertility in age gene rice varieties, be to make recurrent parent, carry out back cross breeding; (3) select the different genotype photo-thermo sensitive male nuclear sterile rice line phase mutual cross with strain regulatory fertility in age gene or reestablish diplomatic relations for use; (4) select for use the photo-thermo sensitive male nuclear sterile rice line with strain regulatory fertility in age gene and rice varieties with strain regulatory fertility in age gene, be hybridization or reestablish diplomatic relations; The hybridization back is by the separation reorganization of gene, from the offspring, the photoperiod-temperature sensitive genie male-sterile line individual plant with obvious strain regulatory fertility in age characteristic is selected, in the selection in per generation, the strain of growing directly from seeds is an object with the low sterile strain of sterile light temperature critical value, and old strain regeneration strain in age is an object with the high solid strain of sterile light temperature critical value.Selected individual plant is proceeded the choosing of continuous multi-generation individual plant system, after selecting the stable photo-thermo sensitive male nuclear sterile rice line that isozygotys with strain regulatory fertility in age characteristic, identify and analyze the significance of difference by under climatic cabinate and natural environment, the strain of growing directly from seeds of breeding photo-thermo sensitive male nuclear sterile rice line being made sterile light temperature critical value with old strain regeneration strain in age again, the sterile light temperature of old strain regeneration strain in age critical value is than grow directly from seeds plant height and significant difference, determine that then the photo-thermo sensitive male nuclear sterile rice line of breeding has strain regulatory fertility in age characteristic, but the photo-thermo sensitive male nuclear sterile rice line of called after strain regulatory fertility in age.
Main feature of the present invention is, general photo-thermo sensitive male nuclear sterile rice line only has the photoperiod-temperature sensitive genie male-sterile line gene, and the photo-thermo sensitive male nuclear sterile rice line of strain regulatory fertility in age is except that tool photoperiod-temperature sensitive genie male-sterile line gene, also have strain regulatory fertility in age gene concurrently, and emphasize the intensity of strain regulatory fertility in age characteristic, be to assemble more strain regulatory fertility in age gene as far as possible in the photo-thermo-sensitive genic male sterile line of tool strain regulatory fertility in age characteristic, make this characteristic intensity bigger, the sterile temporary dividing value does not differ greatly between homophyletic plant in age, just has more practicality.
Advantage of the present invention and effect: because its sterile light temperature critical value of photo-thermo-sensitive genic male sterile line of tool strain regulatory fertility in age characteristic has the advantages that to be subjected to strain regulation and control in age, thereby can utilize the low strain of growing directly from seeds of sterile light temperature critical value to carry out the safe production of hybrid seeds, can utilize the high old strain regeneration strain in age of sterile light temperature critical value to carry out numerous kind of high yield again.Solved then numerous kind of difficulty of the production of hybrid seeds simultaneously of local same practical male sterile line and numerous kind or production of hybrid seeds safety, the numerous kind of easy production of hybrid seeds contradiction of safety has inadequately then been removed condition restriction and trouble that practical male sterile line needs relocation propagation or cold-penetration to breed from.In new sterile line seed selection process, can strengthen the sterile temporary dividing value gap of grow directly from seeds strain and old strain regeneration strain in age by selection, the breeding goal of the sterile light temperature of the strain of growing directly from seeds critical value is even lower, more help the safe production of hybrid seeds, simultaneously can be on the spot in the seed selection process numerous kind add generation, accelerate breeding process.Thereby the utilization by this invention, can reduce the risk of the numerous production of hybrid seeds of two-line hybrid rice and save the manpower and materials cost of a large amount of numerous production of hybrid seeds and new sterile line seed selection.
Specific embodiment
Embodiment one: account for the combination of reestablishing diplomatic relations from W7415S/C71140//seven mountains, the paddy rice temperature Photosensitive caryon sterile line N9S of obvious strain regulatory fertility in age characteristic is provided in seed selection.W7415S determines that through screening the strain of growing directly from seeds of the indica type photo-thermo sensitive male nuclear sterile rice line that has strain regulatory fertility in age gene, derive from the academy of agricultural sciences, Hubei, the sterile temperature threshold value ratio of its old strain regeneration strain in age is high approximately 1 ℃ from more than 20 photo-thermo sensitive male nuclear sterile rice lines.C71140 determines the rice variety that has strain regulatory fertility in age gene, derive from Taiwan from more than 100 rice varieties, being through screening.It is the rice variety that the academy of agricultural sciences, Guangdong Province breeds that seven mountains account for.Account for 3 parents by multiple cross, from F with W7415S, C71140, seven mountains
2In generation, begin the sterile strain of growing directly from seeds of selecting sterile light temperature critical value low, and selects the high old strain regeneration strain in age of sterile light temperature critical value after staying the regeneration of standing grain head, through continuous 8 generations individual plant select 1 stable homozygous lines that comprehensive proterties is good after being to select, name and be N9S.Place the climatic cabinate warm combined treatment of not sharing the same light to reach and under natural environment, do anniversary fertility observation simultaneously grow directly from seeds strain and the old strain regeneration strain in age of N9S, the sterile temperature threshold value of the strain of growing directly from seeds that identifies N9S is 23 ℃, sooner or later two seasons can the safe production of hybrid seeds in Guangzhou, sterile temperature threshold value 25-26 ℃ of the old strain regeneration strain in age of N9S, but at two season of Guangzhou morning and evening breeding seed.Illustrating that N9S has more strain regulatory fertility in age gene, is the paddy rice temperature Photosensitive caryon sterile line of the obvious strain of tool regulatory fertility in an age characteristic.
Embodiment two: from W7415S/C71140 hybrid combination, the paddy rice low thermo-sensitive type caryon sterile line N26S of obvious strain regulatory fertility in age characteristic is provided in seed selection.W7415S determines that through screening the strain of growing directly from seeds of the indica type photo-thermo sensitive male nuclear sterile rice line that has strain regulatory fertility in age gene, derive from the academy of agricultural sciences, Hubei, the sterile temperature threshold value ratio of its old strain regeneration strain in age is high approximately 1 ℃ from more than 20 photo-thermo sensitive male nuclear sterile rice lines.C71140 determines the rice variety that has strain regulatory fertility in age gene, derive from Taiwan from more than 100 rice varieties, being through screening.With W7415S and C71140 hybridization, from F
2In generation, begin the sterile strain of growing directly from seeds of selecting sterile light temperature critical value low, and selects the high old strain regeneration strain in age of sterile light temperature critical value after staying the regeneration of standing grain head, through continuous 6 generations individual plant select 1 stable homozygous lines that comprehensive proterties is good after being to select, name and be N26S.Place the climatic cabinate warm combined treatment of not sharing the same light to reach and under natural environment, do anniversary fertility observation simultaneously grow directly from seeds strain and the old strain regeneration strain in age of N26S, identify about 23 ℃ of the sterile temperature threshold value of the strain of growing directly from seeds of N26S, sooner or later two seasons can the safe production of hybrid seeds in Guangzhou, about 25 ℃ of the sterile temperature threshold value of old strain regeneration strain in age of N26S, but at two season of Guangzhou morning and evening breeding seed.Illustrating that N26S has more strain regulatory fertility in age gene, is the paddy rice low thermo-sensitive type caryon sterile line of the obvious strain of tool regulatory fertility in an age characteristic.
Claims (3)
1, the photo-thermo sensitive male nuclear sterile rice line selection of strain regulatory fertility in age, it is characterized in that screening photo-thermo sensitive male nuclear sterile rice line with strain regulatory fertility in age gene, whether variant by the strain of under same ecotope, relatively growing directly from seeds with the sterile light temperature critical value of old strain regeneration strain in age, if the sterile light temperature critical value of old strain regeneration strain in age, determines then that this photo-thermo sensitive male nuclear sterile rice line has strain regulatory fertility in age gene obviously than the plant height that grows directly from seeds; Again from the rice varieties of different genotype, be with photo-thermo-sensitive genic male sterile line, by with rice varieties, be and do not have the photo-thermo sensitive male nuclear sterile rice line hybridization of strain regulatory fertility in age gene, again from filial generation F
2Select the photoperiod-temperature sensitive genie male-sterile line strain to differentiate whether have strain regulatory fertility in age characteristic in the colony, as have this characteristic, determine that then this rice varieties, cording have strain regulatory fertility in age gene, filter out the different germplasms with strain regulatory fertility in age gene; Select for use thereafter through screening have the germplasm of strain regulatory fertility in age gene and different genotype rice varieties, be or photo-thermo-sensitive genic male sterile line mutual cross mutually that cross method comprises: (1) is selected rice varieties with strain regulatory fertility in age gene for use, is to hybridize with photo-thermo-sensitive genic male sterile line; (2) with photo-thermo sensitive male nuclear sterile rice line with have strain regulatory fertility in age gene rice varieties, be hybridization, then with have strain regulatory fertility in age gene rice varieties, be to make recurrent parent, carry out back cross breeding; (3) select the different genotype photo-thermo sensitive male nuclear sterile rice line phase mutual cross with strain regulatory fertility in age gene or reestablish diplomatic relations for use; (4) select for use the photo-thermo sensitive male nuclear sterile rice line with strain regulatory fertility in age gene and rice varieties with strain regulatory fertility in age gene, be hybridization or reestablish diplomatic relations; The hybridization back is by the separation reorganization of gene, from the offspring, the photoperiod-temperature sensitive genie male-sterile line individual plant with obvious strain regulatory fertility in age characteristic is selected, in the selection in per generation, the strain of growing directly from seeds is an object with the low sterile strain of sterile light temperature critical value, and old strain regeneration strain in age is an object with the high solid strain of sterile light temperature critical value.Selected individual plant is proceeded the choosing of continuous multi-generation individual plant system, after selecting the stable photo-thermo sensitive male nuclear sterile rice line that isozygotys with strain regulatory fertility in age characteristic, identify and analyze the significance of difference by under climatic cabinate and natural environment, the strain of growing directly from seeds of breeding photo-thermo sensitive male nuclear sterile rice line being made sterile light temperature critical value with old strain regeneration strain in age again, the sterile light temperature of old strain regeneration strain in age critical value is than grow directly from seeds plant height and significant difference, determine that then the photo-thermo sensitive male nuclear sterile rice line of breeding has strain regulatory fertility in age characteristic, but the photo-thermo sensitive male nuclear sterile rice line of called after strain regulatory fertility in age.
2, according to the selection of the described tool strain of claim 1 regulatory fertility in age characteristic water rice photo-thermo-sensitive nucleic male sterile line, the rice photo-thermo-sensitive type caryon sterile line called after N9S that it is characterized in that tool strain regulatory fertility in the age characteristic that filters out, be to utilize the rice variety C71140 of the photo-thermo sensitive male nuclear sterile rice line W7415S of tool strain regulatory fertility in age gene and tool strain regulatory fertility in age gene and rice variety seven mountains to account for to be the parent, come out by the method seed selection of multiple cross.
3, according to the selection of the described tool strain of claim 1 regulatory fertility in age characteristic water rice photo-thermo-sensitive nucleic male sterile line, the paddy rice low thermo-sensitive type caryon sterile line called after N26S that it is characterized in that tool strain regulatory fertility in the age characteristic that filters out, be to utilize the photo-thermo sensitive male nuclear sterile rice line W7415S of tool strain regulatory fertility in age gene and the rice variety C71140 of tool strain regulatory fertility in age gene to be the parent, come out by the method seed selection of single cross.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB021151539A CN1167331C (en) | 2002-04-27 | 2002-04-27 | Method for breeding light- and temp.-sensitive nucleus sterile line rice with plant age regulatory fertility |
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| CNB021151539A CN1167331C (en) | 2002-04-27 | 2002-04-27 | Method for breeding light- and temp.-sensitive nucleus sterile line rice with plant age regulatory fertility |
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| CN1167331C true CN1167331C (en) | 2004-09-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155852A (en) * | 2011-12-13 | 2013-06-19 | 天津天隆种业科技有限公司 | Technical method for cultivating optical thermosensitive genetic male sterile line with low fertility transformation starting point temperature |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100457905C (en) * | 2006-05-24 | 2009-02-04 | 华南农业大学 | Paddy rice hybrid fertility gene and its application |
| CN102884976B (en) * | 2012-08-14 | 2014-02-05 | 武汉大学 | Method for breeding hybrid rice photosensitive (thermosensitive) male sterile line |
| CN110199803B (en) * | 2019-06-25 | 2022-10-25 | 华南农业大学 | Identification method for effective low-temperature accumulation effect of two-line sterile line rice fertility conversion |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155852A (en) * | 2011-12-13 | 2013-06-19 | 天津天隆种业科技有限公司 | Technical method for cultivating optical thermosensitive genetic male sterile line with low fertility transformation starting point temperature |
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