CN100457905C - Paddy rice hybrid fertility gene and its application - Google Patents
Paddy rice hybrid fertility gene and its application Download PDFInfo
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Abstract
The invention relates to the separation cloning and application of paddy hybrid gene Sb. It includes the paddy hybrid gene DNA sequence, the coding protein, and the DNA sequence carrier, and the host cell. The invention also relates to the method and application of the cultivation the compatible series by using the series and the molecule mark and the application to identify the gene.
Description
Technical field
The present invention relates to the genetically engineered field, be specifically related to a kind of separating clone and application in the paddy rice cross breeding breeding thereof of paddy rice hybrid fertility gene.
Background technology
Plant hybridizes by the hybridization between different varieties.Hybrid has advantage at aspects such as growth potential, adaptability, output, is called hybrid vigour.Utilize hybrid vigour can improve output, quality and the resistance of farm crop.The long-grained nonglutinous rice (indica) and japonica rice (japonica) inter subspecific hybrid of cultivated rice (Oryza sativa L.) have powerful hybrid vigour, and yield potential is very big.On the other hand,, can import cultivated rice, cultivate good new variety being present in some the useful proterties of wild-rice such as disease-resistant, pest-resistant, high yield etc. by hybridization of cultivated rice and wild-rice and breeding method such as backcross.But there is the low or sterile problem of fertility in the hybrid that Hybrids of Indica and Japonica and cultivated rice and wild-rice hybridization produce, is called hybrid dysgenesis (hybrid sterility).Described hybrid dysgenesis is one of isolated mechanism of reproduction that produces in the Oryza species atomization, belongs to behind the zygote reproduction and isolates (postzygotic isolation).The hybrid dysgenesis phenomenon has seriously hindered the utilization of rice heterosis and the useful gene of wild-rice.
Found that the locus more than 20 is relevant with paddy rice hybrid fertility; In these hybrid fertile gene seats, some is to cause megagamete sterile, as S5, and S7, S8, S9, S15, S17, some is to cause microgamete (pollen) sterile, as Sa, Sb, Sc, Sd, Se, (Kinoshita 1995, Rice Genet.Newsl., 12:9-15 for Sf; Ikehashi etc. 1988, Japan J.Breed.38:283-291; Ikehashi etc. 1996, Rice Genet III, pp404-408; Wang etc. 1996, Theor Appl Genet 92:183-190; Wang etc. 1998, Rice Genet Newsl15:151-154; Zhang Guiquan etc. 1989, rice in China science 3:97-101; Zhang Guiquan etc. 1993, Acta Genetica Sinica 20:222-228; Zhang Guiquan etc. 1993, Acta Genetica Sinica 20:541-551; Zhang Guiquan etc. 1994, Acta Genetica Sinica 21:34-41).These genes can have variation differentiation in various degree in different varieties or strain or wild-rice material, produce multiple multiple allelomorphos.According to the paddy rice subspecies type of carrying these multiple allelomorphoss and to the effect of hybrid fertile, they are divided into indica type (S
i), round-grained rice type (S
j), affinity type (S
n) or other transitional type.Described gene symbol S represents hybrid sterility (Sterility), S5, and Sa, Sb etc. refer to specific hybrid fertile gene seat,
i,
j,
nRepresent the multiple allelomorphos of indica type (indica), round-grained rice type (japonica) and neutral type (neutral) respectively, as Sb
i, Sb
j, or Sb
nNeutral type is also referred to as affinity type (compatibility) or wide affinity type (wide compatibility).
In paddy rice hybrid, not affine mutual work often takes place in the bigger hybrid fertile gene of differentiation degree under heterozygous state, cause carrying a certain allelic microgamete (or megagamete) grow undesired.This phenomenon is also referred to as the allelotrope selectivity of hybrid and kills the gamete effect.But these genes under homozygotic state (as the parent of isozygotying) do not influence the normal development of gamete.The genetic behavior of most of hybrid dysgenesis locus meets the pattern (Ikehashi etc. 1986, IRRI (ed) Rice Genetics:pp119-130) of " single seat position sporinite-gametophyte is done mutually ".For example, with carrying Sb respectively
jb
jAnd Sb
iSb
iGenotypic paddy rice japonica rice and near isogenic line hybridization, the genotype of the first generation of hybrid of generation (F1) is Sb
iSb
jIn the microgamete of its generation, carry Sb
iAllelicly normally can educate, and carry Sb
jAllelic most of microgamete heteroplasia (sterile) causes F2 for carrying Sb in the colony
jSb
jThe ratio of genotypic plant is seriously on the low side, i.e. substantial deviation Sb
iSb
i: Sb
iSb
j: Sb
jSb
j=1: 2: 1 law of segregation.But, with carrying affinity type allelotrope Sb
nSb
nThe parent with carry Sb
iSb
iOr Sb
jSb
j(genotype of F1 is Sb in allelic parental rice hybridization
nSb
iOr Sb
nSb
j), F2 meets 1: 2: 1 law of segregation for 3 kinds of genotypic separation in the colony, and promptly the allelotrope of this heterozygosis does not produce selectivity and kills gamete effect, Sb
nTo indica type and round-grained rice type allelotrope all is affine.
The heterozygosis S at different seats
iS
jGene can produce selectivity respectively and kill the gamete effect, therefore has the heterozygosis S at a plurality of seats in hybrid
iS
jCan produce the effect that adds up during gene, make hybrid dysgenesis even more serious.Otherwise,, just can improve the fertility of paddy rice hybrid if make hybrid strain carry affinity type allelotrope or phase isoallele at S locus as much as possible.For example, use wide affine system (kind) to be parent and long-grained nonglutinous rice or japonica rice hybridization in the present paddy rice cross breeding breeding usually, the fertility of its hybrid is improved to some extent.Described wide affine strain (kind) is meant at a plurality of hybrid dysgenesis locus carries the allelic strain of affinity type (kind).But available wide affine strain (kind) is less, and majority belongs to the intermediate type of the non-round-grained rice of non-Xian, and hybrid vigour is strong inadequately, and its economical character can not satisfy the diversified requirement of modern rice breeding.
Reported at present the chromosomal localization of some paddy rice hybrid fertility genes, but the separated clone's of paddy rice hybrid fertility gene report was not arranged before the present patent application proposes as yet, the molecular basis of hybrid dysgenesis or affine (can educate) is not clear.Sb, or be named as f5, be one of gene that influences rice hybrid pollen fertility, it has been located in the 5th karyomit(e) (Chuxiong, the village etc. 2002, Acta Genetica Sinica 29:700-705; Li Wentao etc. 2002, Botany Gazette 44:463-467; Li Wentao etc. 2006, Science Bulletin 51:404-408; Wang etc. 2006, Theor.Appl.Genet.112:382-387).In order in breeding of hybridized rice, to overcome the hybrid sterility problem effectively, the present invention has obtained a plurality of allelotrope of paddy rice hybrid fertility gene Sb by map based cloning (map-based cloning) technology, comprise affinity type allelotrope, verified the function of this gene by transgenic experiments, and proposed by the crossbreed method of the affine system of fertility of genetic engineering technique or hybridization-back cross breeding.
Summary of the invention
(1) goal of the invention
The objective of the invention is separating clone paddy rice hybrid fertility gene and the dna fragmentation that comprises the promotor of regulating and control this gene.
Second purpose of the present invention provides the coded protein of above-mentioned hybrid fertile gene.
The 3rd purpose of the present invention provides the application of above-mentioned hybrid fertile gene in the affine system of the fertility that crossbreeds.
A further object of the present invention provides molecule marker or its close linkage mark and the application in the paddy rice cross breeding breeding thereof that above-mentioned hybrid fertile gene produces.
(2) technical scheme
The present invention relates to separate and use a kind of dna fragmentation that comprises hybrid fertile gene Sb, this fragment is given between rice varieties (being) or the microgamete of the hybrid that paddy rice and wild-rice intermolecular hybrid produce has normal fertility.
The present invention utilizes the map based cloning method to clone the Sb gene, and its technological step is as follows:
1, the chain location of the screening of polymorphic molecular marker and Sb gene
At present Sb is positioned approximate region (Chuxiong, the village etc. 2002, Acta Genetica Sinica 29:700-705) on paddy rice the 5th karyomit(e).According to the data (http://www.ncbi.nlm.nih.gov) in disclosed Public Rice Genome Sequence Data storehouse, in this regional development a plurality of molecule markers.Pass through linkage analysis, find 6 and Sb close linkage or common isolating polymorphism mark, and the interval of Sb Fine Mapping at a 5kb.
2, dna sequence analysis
According to the data (http://www.ncbi.nlm.nih.gov) in disclosed Public Rice Genome Sequence Data storehouse, in localized 5kb interval, has only the gene of 1 prediction.
3, the functional verification of Sb gene
With PCR method from 1 affine strain WS59 amplification affinity type allelotrope Sb
n, it comprises promoter region, coding region and downstream sequence, makes up the Plant Transformation expression vector.Transform japonica rice T65 with the soil agrobacterium tumefaciens-mediated transformation, again with near isogenic line E2 hybridization, the F1 that hybridizes, its genotype is Sb
iSb
j/ Sb
n-, Sb wherein
iSb
jBe the endogenous Sb genotype of hybrid, Sb
nBe the transgenosis that imports.Import Sb
nThe fertile flower powder rate of hybrid plant improve, among its self progeny F2, contain Sb
jSb
jThe ratio of genotypic plant improves greatly, shows most of Sb
jThe fertility restorer of type microgamete is normal.These results have proved Sb
nAllelotrope has the biological function that overcomes hybrid dysgenesis.
4, the sequencing comparative analysis of Sb multiple allelomorphos
Amplify the Sb gene fragment with round pcr from a plurality of indica type, round-grained rice types and affinity type kind (being), measure its sequence after being cloned into plasmid vector.Wherein the nucleotide sequence of 3 affinity type genes is shown in sequence table SEQ ID NO:1, SEQ ID NO:3 and SEQ ID NO:5.They are encoded respectively and have the amino acid polypeptide sequence shown in SEQ ID NO:2, SEQ ID NO:4 and the SEQ ID NO:6, and its biological function is the pollen fertility of controlling plant hybrid.These multiple allelomorphoss are all encoded and are contained 662 amino acid whose protein, do not have the conserved domain of known functional protein matter, do not find the gene of homology with it and identical function.The variation of several bases is arranged in the coding region between the described multiple allelomorphos, and cause several amino acid whose variations (table 2).Not affine or affine mutual work takes place in the microgamete that these variations have caused containing the hybrid of various combination heterozygous genes.
According to Sb gene order information provided by the invention, those skilled in the art can easily obtain identical with Sb or are equal to the gene of Sb by following or other genetic engineering technique, and perhaps its function is equivalent to the subfragment of sequence shown in SEQ ID NO:1 or SEQ ID NO:3 or the SEQ ID NO:5: genomic library or cDNA library that (1) classifies the probe Screening of Rice as with Sb gene or homologous fragment or its other adjacent genome sequence obtain; (2) design oligonucleotides primer (containing degenerated primers) increases from the genome of cultivated rice or wild-rice or cDNA through polymerase chain reaction (PCR) and to obtain; (3) modifying transformation with gene engineering method on the basis of Sb gene order obtains; (5) method with chemosynthesis obtains.The described gene that is equal to or is equivalent to Sb, be meant that its encoded protein matter sequence compares the difference of having only one or several amino-acid residue with the amino acid residue sequence shown in SEQ ID NO:2, SEQID NO:4 or the SEQ ID NO:6, comprise replacement, the disappearance of amino-acid residue or add.And these difference do not cause its function and the function of Sb gene provided by the invention that in essence different are arranged.Especially, shown in embodiment provided by the invention, the Sb gene in Different Rice Varieties or strain and the different wild seed rice can exist variation in various degree, i.e. multiple allelomorphos.These multiple allelomorphoss can easily obtain with aforesaid method according to information disclosed by the invention.
The present invention includes such protein sequence, the aminoacid sequence shown in itself and SEQ ID NO:2, SEQ ID NO:4 or the SEQ ID NO:6 has about at least 70% or higher homology, and fertility that can the controlling plant hybrid.
The present invention also comprises the host cell that contains described nucleotide sequence or its segmental cloning vector or expression vector, contain described carrier, contains described nucleotide sequence or its segmental plant transformed cell and transgenic plant.
Hybrid fertile gene provided by the invention has important use and is worth.One of application is the affine system of fertility that crossbreeds.Therefore the present invention also provides the method for cultivating the affine system of paddy rice hybrid fertility by genetic engineering technique or the hybridization-method of backcrossing, and comprise the affine system of paddy rice hybrid fertility and other rice varieties or the incross of further using acquisition, cultivate the affine system of hybrid fertile that makes new advances.Of the present invention gene constructed in plant conversion carrier, can do suitably to modify to described gene or its regulating and controlling sequence, also can before its transcription initiation codon, replace the original promotor of described gene, make it to reach expression effect equal or that strengthen with other promotor.
The Another application of hybrid fertile gene provided by the invention is to produce specific molecule marker or its close linkage mark according to described gene order information, includes but not limited to SNP (mononucleotide polymorphic), InDel (it is polymorphic to insert disappearance), RFLP (restriction enzyme length is polymorphic), CAP (the cutting amplified fragments is polymorphic).Can identify the genotype of cultivated rice or wild-rice or their filial generation colony plant with these marks, use the molecular marker assisted selection breeding, thereby improve the efficiency of selection of breeding.
(3) beneficial effect
According to gene provided by the invention, can be with any plant conversion carrier or method for transformation with described affinity type (Sb
n) allelotrope importing japonica rice or rice variety or strain, the affine system of hybrid fertile of acquisition energy expressing said gene; Or be parent and other rice varieties or incross with the transformed plant that obtains, the affine system of hybrid fertile that cultivation makes new advances.Also can be with carrying Sb
nAllelic cultivated rice or wild-rice material are donor parents, and other japonica rice or rice variety (being) are recurrent parent, hybridize and backcross, and utilize the specific molecular marker that produces according to the present invention to advance screening, cultivate with Sb
nSubstitute original Sb
iOr Sb
jThe affine system of new hybrid fertile.Obtain the affine system of hybrid fertile and other japonica rice or long-grained nonglutinous rice or wild-rice parent hybridization with method of the present invention, can produce the normal hybrid of fertility, help new breed breeding or produce cross-fertilize seed.On the other hand, produce the genotype that molecule marker can be identified paddy rice or wild-rice or filial generation plant, be used for the molecular marker assisted selection breeding, thereby improve the efficiency of selection of breeding with the present invention
Description of drawings
The hereditary pattern of Fig. 1, paddy rice Sb gene and genotype thereof are to the effect of pollen fertility.(A) japonica rice T65 (Sb
jSb
j) the normal fertile flower powder that produces; (B) near isogenic line E2 (Sb
iSb
i) the normal fertile flower powder that produces; (C) the hybrid F of T65 and E2 hybridization
1(Sb
iSb
j) pollen that produces, wherein about 50% pollen sterility (dyeing of arrow indication is more shallow, the pollen that diameter is less); (D-F) F of T65 * E2 hybrid
2In the plant, ratio of different genotype plant and pollen fertility thereof.According to the evaluation with linked marker, F
2Sb in the colony
jSb
jThe genotype plant is seriously on the low side, and F is described
1In the pollen that plant produces, most of Sb
jGenotypic pollen sterility;
The chromosome linkage location of Fig. 2, paddy rice Sb gene, clone, functional analysis and with the crossbreed synoptic diagram of the affine system, method of fertility of transgenic technology;
Fig. 3, with round pcr from different rice material amplification Sb gene fragment.M is a molecular weight marker;
Fig. 4, the expression of usefulness reverse transcription PCR technology for detection Sb gene in young fringe;
The structure of Fig. 5, (A) Sb gene coding region, box indicating exon, the start-stop position in numeral exons coding district (contain and end password).(B) Sb of WS59
nThe allelotrope amino acid sequence coded;
Fig. 6, from the Sb that contains of the affine WS59 of being amplification
nAllelic dna fragmentation is cloned into plant conversion carrier pCAMBIA1300.1-3 is the electrophorogram with 3 clones of BamHI/HindIII double digestion.M is a molecular weight marker;
Fig. 7, with affinity type gene Sb
nImport T65 and obtain the T0 transformant, hybridize the Sb that produces with E2 again
iSb
j/--pollen (the arrow indication is sterile pollen) and the Sb of genotype hybrid (A)
iSb
j/ Sb
nThe pollen of-genotype hybrid (B-D) (major part normally can be educated);
Fig. 8, usefulness single nucleotide polymorphism (SNP) mark 07-81 identify the genotype of parent and hybrid plant (1-6).
Embodiment
Following examples further specify content of the present invention.
Embodiment 1: the screening of polymorphic molecular marker and the Fine Mapping of Sb
Known Sb is positioned at the approximate region (Chuxiong, the village etc. 2002, Acta Genetica Sinica 29:700-705) on paddy rice the 5th karyomit(e).The present invention further clones the Sb gene with map based cloning method as shown in Figure 2.At first use in the japonica rice variety platform 65 (to be called for short T65, to have genotype Sb
jSb
j) and its near isogenic line E2 (Sb
iSb
i) hybridization, set up a F
2For segregating population.The near isogenic line E2 that the present invention is used is except Sb
iOutside the allelotrope, the nuclear gene group background strain similar to T65, so the male infertility of their hybrid only is subjected to Sb
iSb
jHeterozygous genes is done the influence of effect mutually.Use 1%I
2-KI solution-dyed is identified the form of pollen, has determined the F of strain more than 6000
2The pollen fertility phenotype of plant promptly can be educated phenotype or half sterile phenotype (Fig. 1), entirely with these F
2Plant is used for the Fine Mapping of Sb.
According to the data (http://www.ncbi.nlm.nih.gov) in disclosed Public Rice Genome Sequence Data storehouse, in this regional development a plurality of polymorphic molecular markers, as shown in table 1.
The recombination event that molecule marker and target are recovered between the locus is many more, represent that both genetic distances are far away more, otherwise its genetic distance is near more.Utilize the method and the above-mentioned F of strain more than 6000 of this genetic mapping
2Plant has made up the genetic linkage map (Fig. 2) of a Sb gene.It is to have only 1 recombination event between 07-78 and 07-83 and the Sb respectively that 2 marks are arranged among the figure, and mark 07-81 and Sb gene to be zero reorganization promptly be divided into from.Physical distance between 07-78 and the 07-83 is about 5kb.Described locating area be positioned at disclosed paddy rice bacterial artificial chromosome (bacterial artificial chromosome, BAC) P0008A07 (
Http:// www.ncbi.nlm.nih.gov) about 78-83kb place.
Table 1 is with the Sb close linkage or be total to isolating polymorphic molecular marker
07-55 in the table 1 and 07-130 are insertion/disappearance (InDel) mark; All the other are single nucleotide polymorphism (SNP) mark.The SNP detection method of PCR-based is: add the 1ul paddy DNA in 20ul PCR reaction solution, with the primer " F " shown in the table 1 and " R " amplification 33 circulations (94 ℃ of 30s, 63 ℃ of 1min, 72 ℃ of 1min).Get the template that 0.5ul PCR product is the 2nd PCR then, each sample 2 PCR reaction of preparation (20ul), promptly use primer " F " and " R-i " to be the specific reaction of raq gene type, with primer " F " and " R-j " is the genotypic specific reaction of T65,8-10 circulation (94 ℃ of 30s increase, 58 ℃ of 1min, 72 ℃ of 1min).Detect the PCR product with 1% sepharose.
The clone of embodiment 2:Sb and sequential analysis
According to the disclosed paddy rice of GenBank (japonica rice) genomic dna sequence and RiceGAAS software (
Http:// ricegaas.dna.affrc.go.jp) analyze, in localized 5kb zone, only there is the gene of 1 prediction.1 662 amino acid whose protein of this genes encoding does not have the conserved domain of known functional protein matter.The database contrast (
Http:// www.ncbi.nlm.nih.gov, blastn, blastp) do not find the gene of homology with it and identical function therefore to the present invention is to provide a new functional gene.
From 3 affine systems (WS59, Dular, 02428), near isogenic line (E2), rice variety (93-11), and japonica rice variety (T65, Japan is fine) extracts total DNA.DNA of plants trace extraction method is: get the about 0.2g of blade, put into the centrifuge tube of 2ml, add liquid nitrogen, with Phillips screwdriver blade is stampped powdered, add 0.8ml extracting solution (0.5MNaCl, 0.1M Tris-HCl (pH8.0), 0.05M EDTA, 1.25%SDS) shake up, place 30min for 65 ℃.Add 0.8ml chloroform/primary isoamyl alcohol/ethanol (76: 4: 20), concussion 5min, the centrifugal 5min of 12000rpm.Take out supernatant liquor, add 2 times of volume ethanol deposit D NA, the centrifugal 5min of 12000rpm removes supernatant.With 0.5ml 70% ethanol rinsing precipitation, air-dry, be dissolved in 0.1mlTE solution.Use primer 5 '-CATAC
GGATCCCATACATACCAATACAAACC-3 ' (underscore is BamHI) and 5 '-GTTATG
AAGCTTGACAATGGACCTGACATG-3 ' (underscore is HindIII) amplifies Sb gene fragment (Fig. 3) with round pcr from these paddy DNAs.High-fidelity LA-Taq enzyme (Bao Bio-Engineering Company), 30 circulations of increasing (94 ℃ of 30s, 62 ℃ of 1min, 72 ℃ of 5min) are used in this PCR reaction.After cutting with the BamHI/HindIII enzyme, connect and be cloned into plasmid vector pUC18.Through sequencing analysis, find to have in the coding region between these multiple allelomorphoss the difference of base, and cause 2-12 amino acid whose difference (table 2).
To the expression (Fig. 4) of Sb gene in young fringe tissue, and confirmed the coding region structure of this gene (Fig. 5 A) with primer 5 '-CCGTCTACGACCGGCGGT-3 ' and 5 '-CCTAAACAGCACACTTACAGA-3 ' reverse transcription PCR technology for detection by measuring its cDNA sequence.Affine is the Sb of WS59
nThe allelotrope amino acid sequence coded is shown in Fig. 5 B.
The allelic aminoacid sequence difference of the Sb of many rice varieties of table 2 (being).
Embodiment 3: genetic transformation is identified the function and the application thereof of target gene
To be cloned into double base conversion carrier pCAMBIA1300 (Australian CAMBIA company) from the dna fragmentation (Fig. 3) of the affine WS59 of being amplification, obtain called after pCAMBIA1300-Sb
nExpression vector plasmid (Fig. 6).This fragment comprises Sb
nCoding region and upstream transcription control region and downstream sequence.With electrization this expression vector is imported soil agrobacterium tumefaciens (Agrobacterium tumefaciens) bacterial strain EHA105 commonly used.Transform T65 by agrobacterium tumefaciens-mediated transformation (Hiei etc., Plant are J.6:271-282), obtain T0 for transfer-gen plant (Sb
jSb
j/ Sb
n-), Sb wherein
jSb
jBe native gene, Sb
nFor transforming the gene that imports, Sb
n-be expressed as the transgenosis hemizygote.With transformed plant and E2 hybridization, the hybrid F of generation
1Hybrid has 2 kinds of genotype, i.e. Sb
iSb
j/--(there is not Sb
n) and Sb
iSb
j/ Sb
n-(there is Sb
nHemizygote).
Hybrid F
1Get pollen during heading with 1% potassiumiodide (I
2-KI) dyeing microscopic examination.At Sb
iSb
j/--genotypic hybrid, its fertile flower powder rate is about 50% (Fig. 7 A).Be divided into 3 kinds of genotypic numbers analyzing 434 F2 plant from mark 07-81 with Sb, find Sb
iSb
i, Sb
iSb
j, and Sb
jSb
jThe genotype plant is respectively 205 strains (47.2%), and 218 strains (50.2%) and 11 strains (2.5%) show most Sb
jThe type microgamete is sterile, Sb
jAllelotrope mainly is delivered to the offspring by megagamete.Import Sb and contain
nHybrid F
1(Sb
iSb
j/ Sb
n-) fertile flower powder rate bring up to about 80% (Fig. 7 B-D).Analyze 507 F
23 kinds of genotypic numbers of plant, Sb
iSb
i, Sb
iSb
j, and Sb
jSb
jThe genotype individuality is respectively 158 strains (31.1%), 248 strains (48.9%) and 101 strains (20.0%), Sb
jSb
jThe individual ratio of genotype increases substantially, and shows Sb
jThe fertility of type microgamete obtains to recover.These results have proved Sb
nAllelotrope has the Sb that recovers hybrid
jGenotype is recovered the biological function of fertility.
With affinity type allelotrope Sb
nImport in the japonica rice, and contain indica type allelotrope Sb
iIncross, can obtain the normal hybrid of male fertile.This transgenosis evidence, Sb provided by the invention
nGene is the paddy rice hybrid fertility compatibility gene that function is arranged, and can cultivate the affine system of hybridization fertility by transgenosis and selfing purifying.According to the present invention, similarly also can be with Sb
nGene imports in the long-grained nonglutinous rice, obtains the affine system of hybrid fertile.Also can be with carrying Sb
nAllelic cultivated rice or wild-rice material are donor parents, and other japonica rice or rice variety (being) are recurrent parent, hybridize and backcross, and utilize the specific molecular marker that produces according to the present invention to advance screening, cultivate with Sb
nSubstitute original Sb
iOr Sb
jThe affine system of new hybrid fertile.The affine system of hybrid fertile and other japonica rice or rice variety (being) or wild-rice hybridization and selection with method of the present invention obtains can produce the higher hybrid of fertility, help the new kind of seed selection or produce cross-fertilize seed.
The application of embodiment 4:Sb gene order in the molecular marker assisted selection breeding
Utilize sequence information provided by the invention can produce the allele specific oligonucleotide molecule marker of Sb, be used to identify the plant that has the range gene type on the Sb site, can in the molecular marker assisted selection breeding process, be applied.For example, the mark 07-81 shown in the table 1 results from the Sb gene order, can be used for identifying the genotype (Fig. 8) of each hybrid plant.
Sequence table
<110〉Agricultural University Of South China
<120〉a kind of paddy rice hybrid fertility gene and application thereof
<130>
<160>6
<170>PatentIn?version?3.3
<210>1
<211>4200
<212>DNA
<213〉Oryza rice strain WS59
<220>
<221>CDS
<222>(1292)..(1861)
<220>
<221>CDS
<222>(1996)..(3004)
<220>
<221>CDS
<222>(3090)..(3499)
<400>1
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tcttttcaag?agtaaactgc?acaaacagtc?cctaaacttg?tgatggtgta?tcacataggt 180
ccctaaactc?tcaaaatgta?ttttttgtgt?tcccgaactt?gtctagggtg?tcgtataggt 240
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aaagaatttt?aagagtttag?ggatatagat?gatatactac?gcatgtgcac?ttcactcgtt 600
ttgcaatgtt?gcaattgatt?ttccttttta?tgtttcacgt?gctggggtct?tggtggtgtt 660
gcaacaagta?ttgttgactg?ttgcatctgt?tagaaatatg?catatgcatg?tttcatcgag 720
tgaggaacta?tttgaaacag?ctagcggggt?ttgaaatttc?gtttcaaaat?ttccgaaatt 780
tcagtaatct?catatatagc?ccgaaatttt?cggttttttg?caaatcttta?tgaatttggt 840
caaattttat?tcaaattcat?tcaaaatcag?tcaaaatgtt?aaataatttc?ggccaaaaaa 900
aaaagaaatt?cccgaaattt?ctgtgccccc?ggcagaaata?gcaatttcga?aatttaaaac 960
tgtgagctag?ccaaggaaat?taattaagca?caaacagcgc?agcttagcga?agtgagtgag 1020
tgaaggagtg?cgcgcatcgc?ttcgcctgga?gagaccacct?tcttctccaa?tccccagttt 1080
tctccccttg?ctgcttttgc?ccgcccccct?ctccccccca?aaaaaaacca?aaaaaaaaaa 1140
aagaagaaga?agaagaagaa?gaagaaagga?agaagaaaac?ctagctagtg?tgaatcgcga 1200
gcacttaccg?atgcttcgaa?gggcgacggc?tgcggtggcc?ggccgccgtc?tacgaccggc 1260
ggtgggcctc?tcgccgtcgg?ccccggcgag?a?atg?gtg?atg?ggt?tgc?acc?cac 1312
Met?Val?Met?Gly?Cys?Thr?His
1 5
cag?cag?ctc?gcc?gac?cca?tgg?gca?tgg?gca?gcc?cgc?gag?gaa?ggt?ggc 1360
Gln?Gln?Leu?Ala?Asp?Pro?Trp?Ala?Trp?Ala?Ala?Arg?Glu?Glu?Gly?Gly
10 15 20
cgt?gtc?gcc?ggc?agc?tcc?ctg?cca?cgg?cgg?ccg?acc?cct?acc?acc?gcc 1408
Arg?Val?Ala?Gly?Ser?Ser?Leu?Pro?Arg?Arg?Pro?Thr?Pro?Thr?Thr?Ala
25 30 35
atc?ggc?cgg?gtc?gcc?gcc?ggg?cgc?cgc?cca?cct?gca?ccg?gca?act?ggc 1456
Ile?Gly?Arg?Val?Ala?Ala?Gly?Arg?Arg?Pro?Pro?Ala?Pro?Ala?Thr?Gly
40 45 50 55
cca?ccg?cag?ggg?acg?ccc?gcc?atg?tcg?ctg?gca?cca?cca?cca?cca?aag 1504
Pro?Pro?Gln?Gly?Thr?Pro?Ala?Met?Ser?Leu?Ala?Pro?Pro?Pro?Pro?Lys
60 65 70
gcc?tcg?cct?tcc?gcc?gcc?cgc?ctg?cag?aag?ccg?gca?gct?tcc?tgc?cac 1552
Ala?Ser?Pro?Ser?Ala?Ala?Arg?Leu?Gln?Lys?Pro?Ala?Ala?Ser?Cys?His
75 80 85
ggc?ggc?caa?cca?aag?gcc?tcg?cct?tgg?gcc?ggg?tcg?ccg?ccg?gga?gca 1600
Gly?Gly?Gln?Pro?Lys?Ala?Ser?Pro?Trp?Ala?Gly?Ser?Pro?Pro?Gly?Ala
90 95 100
gcc?acc?cac?ctg?cag?cag?ccg?tcg?cgg?atg?aag?ctc?tct?ccg?tcg?ccg 1648
Ala?Thr?His?Leu?Gln?Gln?Pro?Ser?Arg?Met?Lys?Leu?Ser?Pro?Ser?Pro
105 110 115
gca?cct?ggc?gcc?caa?cac?ctg?cca?ccg?gca?aag?ccc?tcg?cct?ttg?gcc 1696
Ala?Pro?Gly?Ala?Gln?His?Leu?Pro?Pro?Ala?Lys?Pro?Ser?Pro?Leu?Ala
120 125 130 135
ggg?tcg?ccg?ccg?gga?gca?gcc?acc?cac?ctg?cag?cag?ccg?gcg?gcg?agg 1744
Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His?Leu?Gln?Gln?Pro?Ala?Ala?Arg
140 145 150
atg?ctg?gcc?tcg?tcg?ccg?gcg?aag?agg?gtg?ctc?tca?gtc?ttc?tca?cca 1792
Met?Leu?Ala?Ser?Ser?Pro?Ala?Lys?Arg?Val?Leu?Ser?Val?Phe?Ser?Pro
155 160 165
tca?gcg?gcc?ggg?tcg?ctg?cgg?agg?gaa?cgt?aac?cat?ctg?ccg?cca?ttg 1840
Ser?Ala?Ala?Gly?Ser?Leu?Arg?Arg?Glu?Arg?Asn?His?Leu?Pro?Pro?Leu
170 175 180
gcc?agc?tgc?ttc?tgc?ttg?cgg?gtgggtaact?ccaaattcca?aatttaggtc 1891
Ala?Ser?Cys?Phe?Cys?Leu?Arg
185 190
caattaaagg?acccccaatt?tcctcgcttg?tagctagtct?tatcttattt?gggtcatgta 1951
ttcattgact?gatgatttgg?gtcgtgtata?tattgtgttt?gcag?cgg?ctg?atg?agt 2007
Arg?Leu?Met?Ser
acc?gga?cta?cct?caa?att?cca?agt?ccc?cca?gca?aca?ctg?atg?aga?cct 2055
Thr?Gly?Leu?Pro?Gln?Ile?Pro?Ser?Pro?Pro?Ala?Thr?Leu?Met?Arg?Pro
195 200 205 210
tgg?tgt?aat?gag?gat?gag?cag?gtg?cct?caa?cta?acc?cct?ttt?aag?atc 2103
Trp?Cys?Asn?Glu?Asp?Glu?Gln?Val?Pro?Gln?Leu?Thr?Pro?Phe?Lys?Ile
215 220 225
agt?cat?ggt?gtc?ttg?gat?aca?atg?atg?aag?cag?tat?gag?gaa?gac?tac 2151
Ser?His?Gly?Val?Leu?Asp?Thr?Met?Met?Lys?Gln?Tyr?Glu?Glu?Asp?Tyr
230 235 240
aag?tat?ctt?gtg?gag?gag?ggg?ctg?gat?ctt?gaa?gag?atg?aag?aaa?ctt 2199
Lys?Tyr?Leu?Val?Glu?Glu?Gly?Leu?Asp?Leu?Glu?Glu?Met?Lys?Lys?Leu
245 250 255
tac?cat?gag?ctg?gtg?cgt?ccc?gtt?tta?gtt?gca?agc?tgt?ctg?att?ctt 2247
Tyr?His?Glu?Leu?Val?Arg?Pro?Val?Leu?Val?Ala?Ser?Cys?Leu?Ile?Leu
260 265 270
gaa?cat?cac?att?gtc?agt?cct?gaa?acc?aaa?gcg?cta?gat?cag?aaa?ttc 2295
Glu?His?His?Ile?Val?Ser?Pro?Glu?Thr?Lys?Ala?Leu?Asp?Gln?Lys?Phe
275 280 285 290
aaa?gac?aag?ttc?ggg?aaa?ccc?cca?atc?tat?ctc?tca?gct?ctt?gat?ata 2343
Lys?Asp?Lys?Phe?Gly?Lys?Pro?Pro?Ile?Tyr?Leu?Ser?Ala?Leu?Asp?Ile
295 300 305
atg?aag?acc?ttg?att?gct?atg?gag?ccg?tcg?aga?tgg?agt?tac?ctt?atg 2391
Met?Lys?Thr?Leu?Ile?Ala?Met?Glu?Pro?Ser?Arg?Trp?Ser?Tyr?Leu?Met
310 315 320
gat?ttc?tta?aga?gag?aaa?cgt?ata?ttg?gtc?act?gag?aat?tgg?gaa?gtt 2439
Asp?Phe?Leu?Arg?Glu?Lys?Arg?Ile?Leu?Val?Thr?Glu?Asn?Trp?Glu?Val
325 330 335
tca?ctg?ggt?tcc?atc?gag?tgc?atc?atg?cac?gac?gac?agc?att?agt?ttt 2487
Ser?Leu?Gly?Ser?Ile?Glu?Cys?Ile?Met?His?Asp?Asp?Ser?Ile?Ser?Phe
340 345 350
atg?atg?aat?ggc?cgg?gtc?gtt?ttc?aaa?ggg?caa?tct?gat?ggt?agg?tat 2535
Met?Met?Asn?Gly?Arg?Val?Val?Phe?Lys?Gly?Gln?Ser?Asp?Gly?Arg?Tyr
355 360 365 370
aac?cat?ggg?act?ggt?aca?gcc?gat?ctt?tgc?aca?gcg?att?act?ggt?gca 2583
Asn?His?Gly?Thr?Gly?Thr?Ala?Asp?Leu?Cys?Thr?Ala?Ile?Thr?Gly?Ala
375 380 385
gct?aca?gat?atc?gtt?gat?tgt?gcc?atc?tac?cga?gag?gtc?cct?tgc?aat 2631
Ala?Thr?Asp?Ile?Val?Asp?Cys?Ala?Ile?Tyr?Arg?Glu?Val?Pro?Cys?Asn
390 395 400
tct?cca?acg?cac?gct?gag?ttg?ctt?gga?atg?tat?gtt?cta?gaa?aga?agg 2679
Ser?Pro?Thr?His?Ala?Glu?Leu?Leu?Gly?Met?Tyr?Val?Leu?Glu?Arg?Arg
405 410 415
gca?ata?tcg?ctg?aaa?ata?ctg?ttg?ttt?gat?gtg?aaa?acc?gac?aac?gct 2727
Ala?Ile?Ser?Leu?Lys?Ile?Leu?Leu?Phe?Asp?Val?Lys?Thr?Asp?Asn?Ala
420 425 430
ttt?gtc?agt?gaa?aca?gtc?aga?gat?atg?ttt?cct?att?act?cca?aac?acc 2775
Phe?Val?Ser?Glu?Thr?Val?Arg?Asp?Met?Phe?Pro?Ile?Thr?Pro?Asn?Thr
435 440 445 450
act?gag?aag?gat?ctg?tgc?caa?gtg?ttg?aga?agt?atg?aaa?gtc?tat?ttc 2823
Thr?Glu?Lys?Asp?Leu?Cys?Gln?Val?Leu?Arg?Ser?Met?Lys?Val?Tyr?Phe
455 460 465
gaa?cac?ttc?aat?tgt?cgg?tgt?gaa?cct?cgg?gag?aaa?ctt?gag?cta?gtg 2871
Glu?His?Phe?Asn?Cys?Arg?Cys?Glu?Pro?Arg?Glu?Lys?Leu?Glu?Leu?Val
470 475 480
gat?agt?tta?atg?aaa?atg?aag?gat?aat?gag?ttg?aca?atg?gaa?tca?atc 2919
Asp?Ser?Leu?Met?Lys?Met?Lys?Asp?Asn?Glu?Leu?Thr?Met?Glu?Ser?Ile
485 490 495
aag?gat?aaa?tgg?gct?cat?tcc?ctt?ctg?aga?ttg?cct?gtc?ttc?agg?gct 2967
Lys?Asp?Lys?Trp?Ala?His?Ser?Leu?Leu?Arg?Leu?Pro?Val?Phe?Arg?Ala
500 505 510
cat?cag?cca?acc?aag?acc?ata?aga?aag?gac?tat?ata?a?gtaagtagtt 3014
His?Gln?Pro?Thr?Lys?Thr?Ile?Arg?Lys?Asp?Tyr?Ile
515 520 525
aatcttctga?gtacattgac?tttgattttt?ttttcttttt?tcttttaacc?catcggttta 3074
atttttatat?tgcag?ac aag?gct?cca?act?gtt?ggt?aca?gta?gct?ttc?cat 3124
Asn?Lys?Ala?Pro?Thr?Val?Gly?Thr?Val?Ala?Phe?His
530 535
ggg?caa?tac?aaa?att?gtc?gca?aag?ggg?gat?aca?gca?aaa?acg?gag?gct 3172
Gly?Gln?Tyr?Lys?Ile?Val?Ala?Lys?Gly?Asp?Thr?Ala?Lys?Thr?Glu?Ala
540 545 550
gtt?gtg?aac?ttg?gtg?cta?tcg?ctt?cag?cca?atg?gtt?gct?tca?gtt?ttc 3220
Val?Val?Asn?Leu?Val?Leu?Ser?Leu?Gln?Pro?Met?Val?Ala?Ser?Val?Phe
555 560 565 570
gtg?gga?aat?cac?atc?tca?gca?atc?tca?gtc?aag?gaa?gag?ata?gaa?aag 3268
Val?Gly?Asn?His?Ile?Ser?Ala?Ile?Ser?Val?Lys?Glu?Glu?Ile?Glu?Lys
575 580 585
ttt?gtc?aag?ggg?tat?tta?ctg?gac?atc?aag?gag?gct?aag?gga?gca?tat 3316
Phe?Val?Lys?Gly?Tyr?Leu?Leu?Asp?Ile?Lys?Glu?Ala?Lys?Gly?Ala?Tyr
590 595 600
gtt?gtt?atc?atg?aaa?ttt?ata?gaa?act?ccc?aat?gca?ggt?ggt?aaa?ata 3364
Val?Val?IIe?Met?Lys?Phe?Ile?Glu?Thr?Pro?Asn?Ala?Gly?Gly?Lys?Ile
605 610 615
tta?gat?gtc?ttg?ttt?aat?act?gaa?gtc?ggt?gag?gtg?tac?aag?cag?aca 3412
Leu?Asp?Val?Leu?Phe?Asn?Thr?Glu?Val?Gly?Glu?Val?Tyr?Lys?Gln?Thr
620 625 630
gca?aac?tcg?gtc?tgc?gtc?aaa?cta?ctg?gac?aag?aat?gat?gga?act?act 3460
Ala?Asn?Ser?Val?Cys?Val?Lys?Leu?Leu?Asp?Lys?Asn?Asp?Gly?Thr?Thr
635 640 645 650
cca?gag?cta?agt?cca?gca?tgt?ctt?ctt?att?ttc?ggt?tgagtcacaa 3506
Pro?Glu?Leu?Ser?Pro?Ala?Cys?Leu?Leu?Ile?Phe?Gly
655 660
agggtcaaac?tagtagtttc?ttggcatgtg?ccttctgtaa?gtgtgctgtt?taggtggctg 3566
gagggccctt?tttggttagt?agtctagacc?ctaacaacat?gttattactt?atgctgctgt 3626
tatgcaccct?gtgcagcacg?cttctgaatt?gcaacttctt?ttcaagctga?atttatctgc 3686
gagtgttaac?cctattttgt?gatggtcata?tatgtttatg?gatggagctt?caagctgggg 3746
catctgcagg?gatctgagta?ccagaactga?agttctttgt?gaaagaagtt?gttttgcttg 3806
tagatggcaa?tttccatgca?gtctcatctg?tacaaccccc?aaaaagcatc?attctgtttc 3866
agctacgagc?tctgtgtttg?tgcttttgtt?tttcttcttg?tttaccccag?gatgggatgg 3926
caatggtagg?gaatgctggt?gtatctagac?cgtgtttgtg?cttttgtttt?tcttcttgtt 3986
taccccagga?tgggacggca?atggtaggga?atgctggtgt?atctagacca?aggtttacaa 4046
actgcacggt?gatcacgcag?ttatcgtgat?tatcacgtgc?aacaaccttc?tcagttttaa 4106
aaccacgtta?tatctccacg?gtaaccttgt?ggttttcatg?ataaccgtga?taaccatcaa 4166
acggtgaggt?ggtggtaacc?caccccaaac?gatt 4200
<210>2
<211>662
<212>PRT
<213〉Oryza rice strain WS59
<400>2
Met?Val?Met?Gly?Cys?Thr?His?Gln?Gln?Leu?Ala?Asp?Pro?Trp?Ala?Trp
1 5 10 15
Ala?Ala?Arg?Glu?Glu?Gly?Gly?Arg?Val?Ala?Gly?Ser?Ser?Leu?Pro?Arg
20 25 30
Arg?Pro?Thr?Pro?Thr?Thr?Ala?Ile?Gly?Arg?Val?Ala?Ala?Gly?Arg?Arg
35 40 45
Pro?Pro?Ala?Pro?Ala?Thr?Gly?Pro?Pro?Gln?Gly?Thr?Pro?Ala?Met?Ser
50 55 60
Leu?Ala?Pro?Pro?Pro?Pro?Lys?Ala?Ser?Pro?Ser?Ala?Ala?Arg?Leu?Gln
65 70 75 80
Lys?Pro?Ala?Ala?Ser?Cys?His?Gly?Gly?Gln?Pro?Lys?Ala?Ser?Pro?Trp
85 90 95
Ala?Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His?Leu?Gln?Gln?Pro?Ser?Arg
100 105 110
Met?Lys?Leu?Ser?Pro?Ser?Pro?Ala?Pro?Gly?Ala?Gln?His?Leu?Pro?Pro
115 120 125
Ala?Lys?Pro?Ser?Pro?Leu?Ala?Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His
130 135 140
Leu?Gln?Gln?Pro?Ala?Ala?Arg?Met?Leu?Ala?Ser?Ser?Pro?Ala?Lys?Arg
145 150 155 160
Val?Leu?Ser?Val?Phe?Ser?Pro?Ser?Ala?Ala?Gly?Ser?Leu?Arg?Arg?Glu
165 170 175
Arg?Asn?His?Leu?Pro?Pro?Leu?Ala?Ser?Cys?Phe?Cys?Leu?Arg?Arg?Leu
180 185 190
Met?Ser?Thr?Gly?Leu?Pro?Gln?Ile?Pro?Ser?Pro?Pro?Ala?Thr?Leu?Met
195 200 205
Arg?Pro?Trp?Cys?Asn?Glu?Asp?Glu?Gln?Val?Pro?Gln?Leu?Thr?Pro?Phe
210 215 220
Lys?Ile?Ser?His?Gly?Val?Leu?Asp?Thr?Met?Met?Lys?Gln?Tyr?Glu?Glu
225 230 235 240
Asp?Tyr?Lys?Tyr?Leu?Val?Glu?Glu?Gly?Leu?Asp?Leu?Glu?Glu?Met?Lys
245 250 255
Lys?Leu?Tyr?His?Glu?Leu?Val?Arg?Pro?Val?Leu?Val?Ala?Ser?Cys?Leu
260 265 270
Ile?Leu?Glu?His?His?Ile?Val?Ser?Pro?Glu?Thr?Lys?Ala?Leu?Asp?Gln
275 280 285
Lys?Phe?Lys?Asp?Lys?Phe?Gly?Lys?Pro?Pro?Ile?Tyr?Leu?Ser?Ala?Leu
290 295 300
Asp?Ile?Met?Lys?Thr?Leu?Ile?Ala?Met?Glu?Pro?Ser?Arg?Trp?Ser?Tyr
305 310 315 320
Leu?Met?Asp?Phe?Leu?Arg?Glu?Lys?Arg?Ile?Leu?Val?Thr?Glu?Asn?Trp
325 330 335
Glu?Val?Ser?Leu?Gly?Ser?Ile?Glu?Cys?Ile?Met?His?Asp?Asp?Ser?Ile
340 345 350
Ser?Phe?Met?Met?Asn?Gly?Arg?Val?Val?Phe?Lys?Gly?Gln?Ser?Asp?Gly
355 360 365
Arg?Tyr?Asn?His?Gly?Thr?Gly?Thr?Ala?Asp?Leu?Cys?Thr?Ala?Ile?Thr
370 375 380
Gly?Ala?Ala?Thr?Asp?Ile?Val?Asp?Cys?Ala?Ile?Tyr?Arg?Glu?Val?Pro
385 390 395 400
Cys?Asn?Ser?Pro?Thr?His?Ala?Glu?Leu?Leu?Gly?Met?Tyr?Val?Leu?Glu
405 410 415
Arg?Arg?Ala?Ile?Ser?Leu?Lys?Ile?Leu?Leu?Phe?Asp?Val?Lys?Thr?Asp
420 425 430
Asn?Ala?Phe?Val?Ser?Glu?Thr?Val?Arg?Asp?Met?Phe?Pro?Ile?Thr?Pro
435 440 445
Asn?Thr?Thr?Glu?Lys?Asp?Leu?Cys?Gln?Val?Leu?Arg?Ser?Met?Lys?Val
450 455 460
Tyr?Phe?Glu?His?Phe?Asn?Cys?Arg?Cys?Glu?Pro?Arg?Glu?Lys?Leu?Glu
465 470 475 480
Leu?Val?Asp?Ser?Leu?Met?Lys?Met?Lys?Asp?Asn?Glu?Leu?Thr?Met?Glu
485 490 495
Ser?Ile?Lys?Asp?Lys?Trp?Ala?His?Ser?Leu?Leu?Arg?Leu?Pro?Val?Phe
500 505 510
Arg?Ala?His?Gln?Pro?Thr?Lys?Thr?Ile?Arg?Lys?Asp?Tyr?Ile?Asn?Lys
515 520 525
Ala?Pro?Thr?Val?Gly?Thr?Val?Ala?Phe?His?Gly?Gln?Tyr?Lys?Ile?Val
530 535 540
Ala?Lys?Gly?Asp?Thr?Ala?Lys?Thr?Glu?Ala?Val?Val?Asn?Leu?Val?Leu
545 550 555 560
Ser?Leu?Gln?Pro?Met?Val?Ala?Ser?Val?Phe?Val?Gly?Asn?His?Ile?Ser
565 570 575
Ala?Ile?Ser?Val?Lys?Glu?Glu?Ile?Glu?Lys?Phe?Val?Lys?Gly?Tyr?Leu
580 585 590
Leu?Asp?Ile?Lys?Glu?Ala?Lys?Gly?Ala?Tyr?Val?Val?Ile?Met?Lys?Phe
595 600 605
Ile?Glu?Thr?Pro?Asn?Ala?Gly?Gly?Lys?Ile?Leu?Asp?Val?Leu?Phe?Asn
610 615 620
Thr?Glu?Val?Gly?Glu?Val?Tyr?Lys?Gln?Thr?Ala?Asn?Ser?Val?Cys?Val
625 630 635 640
Lys?Leu?Leu?Asp?Lys?Asn?Asp?Gly?Thr?Thr?Pro?Glu?Leu?Ser?Pro?Ala
645 650 655
Cys?Leu?Leu?Ile?Phe?Gly
660
<210>3
<211>4200
<212>DNA
<213〉Oryza rice strain Dular
<220>
<221>CDS
<222>(1293)..(1862)
<220>
<221>CDS
<222>(1997)..(3005)
<220>
<221>CDS
<222>(3091)..(3500)
<400>3
aaaatcatcc?tgttgagggt?gttgtagcaa?attttatctt?tttttcatac?atgcaatttc 60
ataaattttt?ataaactgta?atttttttaa?tgtttaggaa?ggagtagtct?ctttgtgttt 120
tcctttcaag?agtaaactgc?acagacagtc?cctaaacttg?tgatggtgta?tcacataggt 180
ccctaaactc?tcaaaatgta?ttttttgtgt?tcccgaactt?gtctagggtg?tcgtataggt 240
ccaaacaagc?gctaatccga?cccatgtgtt?gacttgacat?gtcacaatgg?tttctacatg 300
ttggtgggac?ccacatgtca?atcacataaa?aaaattgaag?gataaaggag?acaaaaatgt 360
ttttttttaa?ttttgaaaaa?aaaagagagg?agagagagag?atgagaaaaa?gatgttttta 420
aatttttatt?ctatgtgtgt?ttgataagtg?ggtcccacta?acacgtaggt?gccactatga 480
tattgtcatg?tcagtgtaca?aacctacatg?acagtcaaga?taagtttgga?gacaagaaga 540
aaagaatttt?aagagtttag?ggatatagat?gatatactac?gcatgtgcac?ttcactcgtt 600
ttgcaatgtt?gcaattgatt?ttccttttta?tgtttcacgt?gctggggtct?tggtggtgtt 660
gcaacaagta?ttgttgactg?ttgcatctgt?tagaaatatg?catatgcatg?tttcatcgag 720
tgaggaacta?tttgaaacag?ctagcggggt?ttgaaatttc?gtttcaaaat?ttccgaaatt 780
tcagtaatct?catatatggc?ccgaaatttt?cggttttttg?caaatcttta?tgaatttggt 840
caaattttat?tcaaattcat?tcaaaatcag?tcaaaatgtt?aaataatttc?ggccaaaaaa 900
aaaaagaaat?tcccgaaatt?tctgtgcccc?cggcagaaat?agcaatttcg?aaatttaaaa 960
ctgtgagcta?gccaaggaaa?ttaattaagc?acaaacagcg?cagcttagcg?aagtgagtga 1020
gtgaaggagt?gcgcgcatcg?cttcgcctgg?agagaccacc?ttcttctcca?atccccagtt 1080
ttctcccctt?gctgcttttg?cccgcccccc?tctccccccc?aaaaaaaacc?aaaaaaaaaa 1140
aaagaagaag?aagaagaaga?agaagaaagg?aagaagaaaa?cctagctagt?gtgaatcgcg 1200
agcacttacc?gatgcttcga?agggcgacgg?ctgcggtggc?cggccgccgt?ctacgaccgg 1260
cggtgggcct?ctcgccgtcg?gccccggcga?ga?atg?gtg?atg?ggt?tgc?acc?cac 1313
Met?Val?Met?Gly?Cys?Thr?His
1 5
cag?cag?ctc?gcc?gac?cca?tgg?gca?tgg?gca?gcc?cgc?gag?gaa?ggt?ggc 1361
Gln?Gln?Leu?Ala?Asp?Pro?Trp?Ala?Trp?Ala?Ala?Arg?Glu?Glu?Gly?Gly
10 15 20
cgt?gtc?gcc?ggc?agc?tcc?ctg?cca?cgg?cgg?ccg?acc?cct?acc?acc?gcc 1409
Arg?Val?Ala?Gly?Ser?Ser?Leu?Pro?Arg?Arg?Pro?Thr?Pro?Thr?Thr?Ala
25 30 35
atc?ggc?cgg?gtc?gcc?gcc?ggg?cgc?cgc?cca?cct?gca?ccg?gca?act?ggc 1457
Ile?Gly?Arg?Val?Ala?Ala?Gly?Arg?Arg?Pro?Pro?Ala?Pro?Ala?Thr?Gly
40 45 50 55
cca?ccg?cag?ggg?acg?ccc?gcc?atg?tcg?ctg?gca?cca?cca?cca?cca?aag 1505
Pro?Pro?Gln?Gly?Thr?Pro?Ala?Met?Ser?Leu?Ala?Pro?Pro?Pro?Pro?Lys
60 65 70
gcc?tcg?cct?tcc?gcc?gcc?cgc?ctg?cag?aag?ccg?gca?gct?tcc?tgc?cac 1553
Ala?Ser?Pro?Ser?Ala?Ala?Arg?Leu?Gln?Lys?Pro?Ala?Ala?Ser?Cys?His
75 80 85
ggc?ggc?caa?cca?aag?gcc?tcg?cct?tgg?gcc?ggg?tcg?cca?ccg?gga?gca 1601
Gly?Gly?Gln?Pro?Lys?Ala?Ser?Pro?Trp?Ala?Gly?Ser?Pro?Pro?Gly?Ala
90 95 100
gcc?acc?cac?ctg?cag?cag?ccg?tcg?cgg?atg?aag?ctc?tct?ccg?tcg?ccg 1649
Ala?Thr?His?Leu?Gln?Gln?Pro?Ser?Arg?Met?Lys?Leu?Ser?Pro?Ser?Pro
105 110 115
gca?cct?ggc?gcc?caa?cac?ctg?cca?ccg?gca?aag?ccc?tcg?cct?ttg?gcc 1697
Ala?Pro?Gly?Ala?Gln?His?Leu?Pro?Pro?Ala?Lys?Pro?Ser?Pro?Leu?Ala
120 125 130 135
ggg?tcg?ccg?ccg?gga?gca?gcc?acc?cac?ctg?cag?cag?ccg?gcg?gcg?agg 1745
Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His?Leu?Gln?Gln?Pro?Ala?Ala?Arg
140 145 150
atg?ctg?gcc?tcg?tcg?ccg?gcg?aag?agg?gtg?ctc?tca?gtc?ttc?tca?cca 1793
Met?Leu?Ala?Ser?Ser?Pro?Ala?Lys?Arg?Val?Leu?Ser?Val?Phe?Ser?Pro
155 160 165
tca?gcg?gcc?ggg?tcg?ctg?cgg?agg?gaa?cgt?aac?cat?ctg?ccg?cca?ttg 1841
Ser?Ala?Ala?Gly?Ser?Leu?Arg?Arg?Glu?Arg?Asn?His?Leu?Pro?Pro?Leu
170 175 180
gcc?agc?tgc?ttc?tgc?ttg?cgg?gtgggtaact?ccaaattcca?aatttaggtc 1892
Ala?Ser?Cys?Phe?Cys?Leu?Arg
185 190
caattaaagg?acccccaatt?tcctcgcttg?tagctagtct?tatcttattt?gggtcatgta 1952
ttcattgact?gatgatttgg?gtcgtgtata?tattgtgttt?gcag?cgg?ctg?atg?agt 2008
Arg?Leu?Met?Ser
acc?gga?cta?cct?caa?att?cca?agt?ccc?cca?gca?aca?ctg?atg?aga?cct 2056
Thr?Gly?Leu?Pro?Gln?Ile?Pro?Ser?Pro?Pro?Ala?Thr?Leu?Met?Arg?Pro
195 200 205 210
tgg?tgt?aat?gag?gat?gag?cag?gtg?cct?caa?cta?acc?cct?ttt?aag?atc 2104
Trp?Cys?Asn?Glu?Asp?Glu?Gln?Val?Pro?Gln?Leu?Thr?Pro?Phe?Lys?Ile
215 220 225
agt?cat?ggt?gtc?tcg?gat?aca?atg?atg?aag?cag?tat?gag?gaa?gac?tac 2152
Ser?His?Gly?Val?Ser?Asp?Thr?Met?Met?Lys?Gln?Tyr?Glu?Glu?Asp?Tyr
230 235 240
aag?tat?ctt?gtg?gag?gag?ggg?ctg?gat?ctt?gaa?gag?atg?aag?aaa?ctt 2200
Lys?Tyr?Leu?Val?Glu?Glu?Gly?Leu?Asp?Leu?Glu?Glu?Met?Lys?Lys?Leu
245 250 255
tac?cat?gag?ctg?gtg?cgt?ccc?gtt?tta?gtt?gca?agc?tgt?ctg?att?ctt 2248
Tyr?His?Glu?Leu?Val?Arg?Pro?Val?Leu?Val?Ala?Ser?Cys?Leu?Ile?Leu
260 265 270
gaa?cat?cac?att?gtc?agt?cct?gaa?acc?aaa?gcg?cta?gat?cag?aaa?ttc 2296
Glu?His?His?Ile?Val?Ser?Pro?Glu?Thr?Lys?Ala?Leu?Asp?Gln?Lys?Phe
275 280 285 290
aaa?gac?aag?ttc?ggg?aaa?ccc?cca?atc?tat?ctc?tca?gct?ctt?gat?ata 2344
Lys?Asp?Lys?Phe?Gly?Lys?Pro?Pro?Ile?Tyr?Leu?Ser?Ala?Leu?Asp?Ile
295 300 305
atg?aag?acc?ttg?att?gct?atg?gag?ccg?tcg?aga?tgg?agt?tac?ctt?atg 2392
Met?Lys?Thr?Leu?Ile?Ala?Met?Glu?Pro?Ser?Arg?Trp?Ser?Tyr?Leu?Met
310 315 320
gat?ttc?tta?aga?gag?aaa?cgt?ata?ttg?gtc?act?gag?aat?tgg?gaa?gtt 2440
Asp?Phe?Leu?Arg?Glu?Lys?Arg?Ile?Leu?Val?Thr?Glu?Asn?Trp?Glu?Val
325 330 335
tca?ctg?ggt?tcc?atc?gag?tgc?atc?atg?cac?gac?gac?agc?att?agt?ttt 2488
Ser?Leu?Gly?Ser?Ile?Glu?Cys?Ile?Met?His?Asp?Asp?Ser?Ile?Ser?Phe
340 345 350
atg?atg?aat?ggc?cgg?gtc?gtt?ttc?aaa?ggg?caa?tct?gat?ggt?agg?tat 2536
Met?Met?Asn?Gly?Arg?Val?Val?Phe?Lys?Gly?Gln?Ser?Asp?Gly?Arg?Tyr
355 360 365 370
aac?cat?ggg?act?ggt?aca?gcc?gat?ctt?tgc?aca?acg?att?act?ggt?gta 2584
Asn?His?Gly?Thr?Gly?Thr?Ala?Asp?Leu?Cys?Thr?Thr?Ile?Thr?Gly?Val
375 380 385
gct?aca?gat?atc?gtt?gat?tgt?gcc?atc?tac?cga?gag?gtc?cct?tgc?aat 2632
Ala?Thr?Asp?Ile?Val?Asp?Cys?Ala?Ile?Tyr?Arg?Glu?Val?Pro?Cys?Asn
390 395 400
tct?cca?acg?cac?gct?gag?ttg?ctt?gga?atg?tat?gtt?cta?gaa?aga?agg 2680
Ser?Pro?Thr?His?Ala?Glu?Leu?Leu?Gly?Met?Tyr?Val?Leu?Glu?Arg?Arg
405 410 415
gca?ata?tcg?ctg?aaa?ata?ctg?ttg?ttt?gat?gtg?aaa?acc?gac?aac?gct 2728
Ala?Ile?Ser?Leu?Lys?Ile?Leu?Leu?Phe?Asp?Val?Lys?Thr?Asp?Asn?Ala
420 425 430
ttt?gtc?agt?gaa?aca?gtc?aga?gat?atg?ttt?cct?att?act?cca?aac?act 2776
Phe?Val?Ser?Glu?Thr?Val?Arg?Asp?Met?Phe?Pro?Ile?Thr?Pro?Asn?Thr
435 440 445 450
act?gag?aag?gat?ctg?tgc?caa?gtg?ttg?aga?agt?atg?aaa?gtc?tat?ttc 2824
Thr?Glu?Lys?Asp?Leu?Cys?Gln?Val?Leu?Arg?Ser?Met?Lys?Val?Tyr?Phe
455 460 465
gaa?cac?ttc?aat?tgt?cgg?tgt?gaa?cct?cgg?gag?aaa?ctt?gag?cta?gtg 2872
Glu?His?Phe?Asn?Cys?Arg?Cys?Glu?Pro?Arg?Glu?Lys?Leu?Glu?Leu?Val
470 475 480
gat?agt?tta?atg?aaa?atg?aag?gat?aat?gag?ttg?aca?atg?gaa?tca?atc 2920
Asp?Ser?Leu?Met?Lys?Met?Lys?Asp?Asn?Glu?Leu?Thr?Met?Glu?Ser?Ile
485 490 495
aag?gat?aaa?tgg?gct?cat?tac?ctt?ctg?aga?ttg?cct?gtc?ttc?agg?gct 2968
Lys?Asp?Lys?Trp?Ala?His?Tyr?Leu?Leu?Arg?Leu?Pro?Val?Phe?Arg?Ala
500 505 510
cat?cag?cca?acc?aag?acc?ata?aga?aag?gac?tat?ata?a?gtaagtagtt 3015
His?Gln?Pro?Thr?Lys?Thr?Ile?Arg?Lys?Asp?Tyr?Ile
515 520 525
aatcttctga?gtacattgac?tttgattttt?ttttcttttt?tcttttaacc?catcggttta 3075
atttttatat?tgcag?ac aag?gct?cca?act?gtt?ggt?aca?gta?gct?ttc?cat 3125
Asn?Lys?Ala?Pro?Thr?Val?Gly?Thr?Val?Ala?Phe?His
530 535
ggg?caa?tac?aaa?att?gtc?gca?aag?agg?gat?aca?gca?aaa?acg?gag?gct 3173
Gly?Gln?Tyr?Lys?Ile?Val?Ala?Lys?Arg?Asp?Thr?Ala?Lys?Thr?Glu?Ala
540 545 550
gtt?gtg?aac?ttg?gtg?cta?tcg?ctt?cag?cca?atg?gtt?gct?tca?gtt?ttc 3221
Val?Val?Asn?Leu?Val?Leu?Ser?Leu?Gln?Pro?Met?Val?Ala?Ser?Val?Phe
555 560 565 570
gtg?gga?aat?cac?atc?tca?gca?atc?tca?gtc?aag?gaa?gag?att?gaa?aag 3269
Val?Gly?Asn?His?Ile?Ser?Ala?Ile?Ser?Val?Lys?Glu?Glu?Ile?Glu?Lys
575 580 585
ttt?gtc?aag?ggg?tat?tta?ctg?gac?atc?aag?gag?gct?aag?gga?gca?tat 3317
Phe?Val?Lys?Gly?Tyr?Leu?Leu?Asp?Ile?Lys?Glu?Ala?Lys?Gly?Ala?Tyr
590 595 600
gtt?gtt?atc?atg?aaa?ttt?ata?gaa?act?ccc?aat?gca?ggt?ggt?aaa?ata 3365
Val?Val?Ile?Met?Lys?Phe?Ile?Glu?Thr?Pro?Asn?Ala?Gly?Gly?Lys?Ile
605 610 615
tta?gat?gtc?ttg?ttt?aat?act?gaa?gtc?ggt?gag?gtg?tac?aag?cag?aca 3413
Leu?Asp?Val?Leu?Phe?Asn?Thr?Glu?Val?Gly?Glu?Val?Tyr?Lys?Gln?Thr
620 625 630
gca?aac?tcg?gtc?tgc?gtc?aaa?cta?ctg?gac?aag?aat?gat?gga?act?act 3461
Ala?Asn?Ser?Val?Cys?Val?Lys?Leu?Leu?Asp?Lys?Asn?Asp?Gly?Thr?Thr
635 640 645 650
cca?gag?cta?agt?cca?gca?tgt?ctt?cgt?att?ttc?ggt?tgagtcacaa 3507
Pro?Glu?Leu?Ser?Pro?Ala?Cys?Leu?Arg?Ile?Phe?Gly
655 660
agggtcaaac?tagtagtttc?ttggcatgtg?ccttctgtaa?gtgtgctgtt?taggtggctg 3567
gagggccctt?tttggttagt?agtctagacc?ctaacaacat?gttattactt?atgctgctgt 3627
tatgcaccct?gtgcagcacg?cttctgaatt?gcaacttctt?ttcaagctga?atttatctgc 3687
gagtgttaac?cctattttgt?gatggtcata?tatgtttatg?gatggagctt?caagctgggg 3747
catctgcagg?gatctgagta?ccagaactga?agttctttgt?gaaagaagtt?gttttgcttg 3807
tagatggcaa?tttccatgca?gtctcatctg?tacaaccccc?aaaaagcatc?attctgtttc 3867
agctacgagc?tctgtgtttg?tgcttttgtt?tttcttcttg?tttaccccag?gatgggatgg 3927
caatggtagg?gaatgctggt?gtatctagac?cgtgtttgtg?cttttgtttt?tcttcttgtt 3987
taccccagga?tgggacggca?atggtaggga?atgctggtgt?atctagacca?aggtttacaa 4047
actgcacggt?gatcacgcag?ttatcgtgat?tatcacgtgc?aacaaccttc?ttaaccttgt 4107
ggttttcatg?ataaccgtga?taaccatcaa?acggtgaggt?ggtggtaacc?caccccaaac 4167
gattcggtga?accttgatct?agacaggtga?tct 4200
<210>4
<211>662
<212>PRT
<213〉Oryza rice strain Dular
<400>4
Met?Val?Met?Gly?Cys?Thr?His?Gln?Gln?Leu?Ala?Asp?Pro?Trp?Ala?Trp
1 5 10 15
Ala?Ala?Arg?Glu?Glu?Gly?Gly?Arg?Val?Ala?Gly?Ser?Ser?Leu?Pro?Arg
20 25 30
Arg?Pro?Thr?Pro?Thr?Thr?Ala?Ile?Gly?Arg?Val?Ala?Ala?Gly?Arg?Arg
35 40 45
Pro?Pro?Ala?Pro?Ala?Thr?Gly?Pro?Pro?Gln?Gly?Thr?Pro?Ala?Met?Ser
50 55 60
Leu?Ala?Pro?Pro?Pro?Pro?Lys?Ala?Ser?Pro?Ser?Ala?Ala?Arg?Leu?Gln
65 70 75 80
Lys?Pro?Ala?Ala?Ser?Cys?His?Gly?Gly?Gln?Pro?Lys?Ala?Ser?Pro?Trp
85 90 95
Ala?Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His?Leu?Gln?Gln?Pro?Ser?Arg
100 105 110
Met?Lys?Leu?Ser?Pro?Ser?Pro?Ala?Pro?Gly?Ala?Gln?His?Leu?Pro?Pro
115 120 125
Ala?Lys?Pro?Ser?Pro?Leu?Ala?Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His
130 135 140
Leu?Gln?Gln?Pro?Ala?Ala?Arg?Met?Leu?Ala?Ser?Ser?Pro?Ala?Lys?Arg
145 150 155 160
Val?Leu?Ser?Val?Phe?Ser?Pro?Ser?Ala?Ala?Gly?Ser?Leu?Arg?Arg?Glu
165 170 175
Arg?Asn?His?Leu?Pro?Pro?Leu?Ala?Ser?Cys?Phe?Cys?Leu?Arg?Arg?Leu
180 185 190
Met?Ser?Thr?Gly?Leu?Pro?Gln?Ile?Pro?Ser?Pro?Pro?Ala?Thr?Leu?Met
195 200 205
Arg?Pro?Trp?Cys?Asn?Glu?Asp?Glu?Gln?Val?Pro?Gln?Leu?Thr?Pro?Phe
210 215 220
Lys?Ile?Ser?His?Gly?Val?Ser?Asp?Thr?Met?Met?Lys?Gln?Tyr?Glu?Glu
225 230 235 240
Asp?Tyr?Lys?Tyr?Leu?Val?Glu?Glu?Gly?Leu?Asp?Leu?Glu?Glu?Met?Lys
245 250 255
Lys?Leu?Tyr?His?Glu?Leu?Val?Arg?Pro?Val?Leu?Val?Ala?Ser?Cys?Leu
260 265 270
Ile?Leu?Glu?His?His?Ile?Val?Ser?Pro?Glu?Thr?Lys?Ala?Leu?Asp?Gln
275 280 285
Lys?Phe?Lys?Asp?Lys?Phe?Gly?Lys?Pro?Pro?Ile?Tyr?Leu?Ser?Ala?Leu
290 295 300
Asp?Ile?Met?Lys?Thr?Leu?Ile?Ala?Met?Glu?Pro?Ser?Arg?Trp?Ser?Tyr
305 310 315 320
Leu?Met?Asp?Phe?Leu?Arg?Glu?Lys?Arg?Ile?Leu?Val?Thr?Glu?Asn?Trp
325 330 335
Glu?Val?Ser?Leu?Gly?Ser?Ile?Glu?Cys?Ile?Met?His?Asp?Asp?Ser?Ile
340 345 350
Ser?Phe?Met?Met?Asn?Gly?Arg?Val?Val?Phe?Lys?Gly?Gln?Ser?Asp?Gly
355 360 365
Arg?Tyr?Asn?His?Gly?Thr?Gly?Thr?Ala?Asp?Leu?Cys?Thr?Thr?Ile?Thr
370 375 380
Gly?Val?Ala?Thr?Asp?Ile?Val?Asp?Cys?Ala?Ile?Tyr?Arg?Glu?Val?Pro
385 390 395 400
Cys?Asn?Ser?Pro?Thr?His?Ala?Glu?Leu?Leu?Gly?Met?Tyr?Val?Leu?Glu
405 410 415
Arg?Arg?Ala?Ile?Ser?Leu?Lys?Ile?Leu?Leu?Phe?Asp?Val?Lys?Thr?Asp
420 425 430
Asn?Ala?Phe?Val?Ser?Glu?Thr?Val?Arg?Asp?Met?Phe?Pro?Ile?Thr?Pro
435 440 445
Asn?Thr?Thr?Glu?Lys?Asp?Leu?Cys?Gln?Val?Leu?Arg?Ser?Met?Lys?Val
450 455 460
Tyr?Phe?Glu?His?Phe?Asn?Cys?Arg?Cys?Glu?Pro?Arg?Glu?Lys?Leu?Glu
465 470 475 480
Leu?Val?Asp?Ser?Leu?Met?Lys?Met?Lys?Asp?Asn?Glu?Leu?Thr?Met?Glu
485 490 495
Ser?Ile?Lys?Asp?Lys?Trp?Ala?His?Tyr?Leu?Leu?Arg?Leu?Pro?Val?Phe
500 505 510
Arg?Ala?His?Gln?Pro?Thr?Lys?Thr?Ile?Arg?Lys?Asp?Tyr?Ile?Asn?Lys
515 520 525
Ala?Pro?Thr?Val?Gly?Thr?Val?Ala?Phe?His?Gly?Gln?Tyr?Lys?Ile?Val
530 535 540
Ala?Lys?Arg?Asp?Thr?Ala?Lys?Thr?Glu?Ala?Val?Val?Asn?Leu?Val?Leu
545 550 555 560
Ser?Leu?Gln?Pro?Met?Val?Ala?Ser?Val?Phe?Val?Gly?Asn?His?Ile?Ser
565 570 575
Ala?Ile?Ser?Val?Lys?Glu?Glu?Ile?Glu?Lys?Phe?Val?Lys?Gly?Tyr?Leu
580 585 590
Leu?Asp?Ile?Lys?Glu?Ala?Lys?Gly?Ala?Tyr?Val?Val?Ile?Met?Lys?Phe
595 600 605
Ile?Glu?Thr?Pro?Asn?Ala?Gly?Gly?Lys?Ile?Leu?Asp?Val?Leu?Phe?Asn
610 615 620
Thr?Glu?Val?Gly?Glu?Val?Tyr?Lys?Gln?Thr?Ala?Asn?Ser?Val?Cys?Val
625 630 635 640
Lys?Leu?Leu?Asp?Lys?Asn?Asp?Gly?Thr?Thr?Pro?Glu?Leu?Ser?Pro?Ala
645 650 655
Cys?Leu?Arg?Ile?Phe?Gly
660
<210>5
<211>4200
<212>DNA
<213〉the Oryza rice strain 02428
<220>
<221>CDS
<222>(1293)..(1862)
<220>
<221>CDS
<222>(1997)..(3005)
<220>
<221>CDS
<222>(3091)..(3500)
<400>5
aaaatcatcc?tgttgagggt?gttgtagcaa?attttatctt?tttttcatac?atgcaatttc 60
ataaattttt?ataaactgta?atttttttaa?tgtttaggaa?ggagtagtct?ctttgtgttt 120
tcctttcaag?agtaaactgc?acagacagtc?cctaaacttg?tgatggtgta?tcacataggt 180
ccctaaactc?tcaaaatgta?ttttttgtgt?tcccgaactt?gtctagggtg?tcgtataggt 240
ccaaacaagc?gctaatccga?cccatgtgtt?gacttgacat?gtcacaatgg?tttctacatg 300
ttggtgggac?ccacatgtca?atcacataaa?aaaattgaag?gataaaggag?acaaaaatgt 360
ttttttttaa?ttttgaaaaa?aaaagagagg?agagagagag?atgagaaaaa?gatgttttta 420
aatttttatt?ctatgtgtgt?ttgataagtg?ggtcccacta?acacgtaggt?gccactatga 480
tattgtcatg?tcagtgtaca?aacctacatg?acagtcaaga?taagtttgga?gacaagaaga 540
aaagaatttt?aagagtttag?ggatatagat?gatatactac?gcatgtgcac?ttcactcgtt 600
ttgcaatgtt?gcaattgatt?ttccttttta?tgtttcacgt?gctggggtct?tggtggtgtt 660
gcaacaagta?ttgttgactg?ttgcatctgt?tagaaatatg?catatgcatg?tttcatcgag 720
tgaggaacta?tttgaaacag?ctagcggggt?ttgaaatttc?gtttcaaaat?ttccgaaatt 780
tcagtaatct?catatatggc?ccgaaatttt?cggttttttg?caaatcttta?tgaatttggt 840
caaattttat?tcaaattcat?tcaaaatcag?tcaaaatgtt?aaataatttc?ggccaaaaaa 900
aaaaagaaat?tcccgaaatt?tctgtgcccc?cggcagaaat?agcaatttcg?aaatttaaaa 960
ctgtgagcta?gccaaggaaa?ttaattaagc?acaaacagcg?cagcttagcg?aagtgagtga 1020
gtgaaggagt?gcgcgcatcg?cttcgcctgg?agagaccacc?ttcttctcca?atccccagtt 1080
ttctcccctt?gctgcttttg?cccgcccccc?tctccccccc?aaaaaaaacc?aaaaaaaaaa 1140
aaagaagaag?aagaagaaga?agaagaaagg?aagaagaaaa?cctagctagt?gtgaatcgcg 1200
agcacttacc?gatgcttcga?agggcgacgg?ctgcggtggc?cggccgccgt?ctacgaccgg 1260
cggtgggcct?ctcgccgtcg?gccccggcga?ga?atg?gtg?atg?ggt?tgc?acc?cac 1313
Met?Val?Met?Gly?Cys?Thr?His
1 5
cag?cag?ctc?gcc?gac?cca?tgg?gca?tgg?gca?gcc?cgc?gag?gaa?ggt?ggc 1361
Gln?Gln?Leu?Ala?Asp?Pro?Trp?Ala?Trp?Ala?Ala?Arg?Glu?Glu?Gly?Gly
10 15 20
cgt?gtc?gcc?ggc?agc?tcc?ctg?cca?cgg?cgg?ccg?acc?cct?acc?acc?gcc 1409
Arg?Val?Ala?Gly?Ser?Ser?Leu?Pro?Arg?Arg?Pro?Thr?Pro?Thr?Thr?Ala
25 30 35
atc?ggc?cgg?gtc?gcc?gcc?ggg?cgc?cgc?cca?cct?gca?ccg?gca?act?ggc 1457
Ile?Gly?Arg?Val?Ala?Ala?Gly?Arg?Arg?Pro?Pro?Ala?Pro?Ala?Thr?Gly
40 45 50 55
cca?ccg?cag?ggg?acg?ccc?gcc?atg?tcg?ctg?gca?cca?cca?cca?cca?aag 1505
Pro?Pro?Gln?Gly?Thr?Pro?Ala?Met?Ser?Leu?Ala?Pro?Pro?Pro?Pro?Lys
60 65 70
gcc?tcg?cct?tcc?gcc?gcc?cgc?ctg?cag?aag?ccg?gca?gct?tcc?tgc?cac 1553
Ala?Ser?Pro?Ser?Ala?Ala?Arg?Leu?Gln?Lys?Pro?Ala?Ala?Ser?Cys?His
75 80 85
ggc?ggc?caa?cca?aag?gcc?tcg?cct?tgg?gcc?ggg?tcg?ccg?ccg?gga?gca 1601
Gly?Gly?Gln?Pro?Lys?Ala?Ser?Pro?Trp?Ala?Gly?Ser?Pro?Pro?Gly?Ala
90 95 100
gcc?acc?cac?ctg?cag?cag?ccg?tcg?cgg?atg?aag?ctc?tct?ccg?tcg?ccg 1649
Ala?Thr?His?Leu?Gln?Gln?Pro?Ser?Arg?Met?Lys?Leu?Ser?Pro?Ser?Pro
105 110 115
gca?cct?ggc?gcc?caa?cac?ctg?cca?ccg?gca?aag?ccc?tcg?cct?ttg?gcc 1697
Ala?Pro?Gly?Ala?Gln?His?Leu?Pro?Pro?Ala?Lys?Pro?Ser?Pro?Leu?Ala
120 125 130 135
ggg?tcg?ccg?ccg?gga?gca?gcc?acc?cac?ctg?cag?cag?ccg?gcg?gcg?agg 1745
Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His?Leu?Gln?Gln?Pro?Ala?Ala?Arg
140 145 150
atg?ctg?gcc?tcg?tcg?ccg?gcg?aag?agg?gtg?ctc?tca?gtc?ttc?tca?cca 1793
Met?Leu?Ala?Ser?Ser?Pro?Ala?Lys?Arg?Val?Leu?Ser?Val?Phe?Ser?Pro
155 160 165
tca?gcg?gcc?ggg?tcg?ctg?cgg?agg?gaa?cgt?aac?cat?cta?ccg?cca?ttg 1841
Ser?Ala?Ala?Gly?Ser?Leu?Arg?Arg?Glu?Arg?Asn?His?Leu?Pro?Pro?Leu
170 175 180
gcc?agc?tgc?ttc?tgc?ttg?cgg?gtgggtaact?ccaaattcca?aatttaggtc 1892
Ala?Ser?Cys?Phe?Cys?Leu?Arg
185 190
caattaaagg?acccccaatt?tcctcgcttg?tagctagtct?tatcttattt?gggtcatgta 1952
ttcattgact?gatgatttgg?gtcgtgtata?tattgtgttt?gcag?cgg?ctg?atg?agt 2008
Arg?Leu?Met?Ser
acc?gga?cta?cct?caa?att?cca?agt?ccc?cca?gca?aca?ctg?atg?aga?cct 2056
Thr?Gly?Leu?Pro?Gln?Ile?Pro?Ser?Pro?Pro?Ala?Thr?Leu?Met?Arg?Pro
195 200 205 210
tgg?tgt?aat?gag?gat?gag?cag?gtg?cct?caa?cta?acc?cct?ttt?aag?atc 2104
Trp?Cys?Asn?Glu?Asp?Glu?Gln?Val?Pro?Gln?Leu?Thr?Pro?Phe?Lys?Ile
215 220 225
agt?cat?ggt?gtc?tcg?gat?aca?atg?atg?aag?cag?tat?gag?gaa?gac?tac 2152
Ser?His?Gly?Val?Ser?Asp?Thr?Met?Met?Lys?Gln?Tyr?Glu?Glu?Asp?Tyr
230 235 240
aag?tat?ctt?gtg?gag?gag?ggg?ctg?gat?ctt?gaa?gag?atg?aag?aaa?ctt 2200
Lys?Tyr?Leu?Val?Glu?Glu?Gly?Leu?Asp?Leu?Glu?Glu?Met?Lys?Lys?Leu
245 250 255
tac?cat?gag?ctg?gtg?cgt?ccc?gtt?tta?gtt?gca?agc?tgt?ctg?att?ctt 2248
Tyr?His?Glu?Leu?Val?Arg?Pro?Val?Leu?Val?Ala?Ser?Cys?Leu?Ile?Leu
260 265 270
gaa?cat?cac?att?gtc?agt?cct?gaa?acc?aaa?gcg?cta?gat?cag?aaa?tcc 2296
Glu?His?His?Ile?Val?Ser?Pro?Glu?Thr?Lys?Ala?Leu?Asp?Gln?Lys?Ser
275 280 285 290
aaa?gac?aag?ttc?ggg?aaa?ccc?cca?atc?tat?ctc?tca?gct?ctt?gat?ata 2344
Lys?Asp?Lys?Phe?Gly?Lys?Pro?Pro?Ile?Tyr?Leu?Ser?Ala?Leu?Asp?Ile
295 300 305
atg?aag?acc?ttg?att?gct?atg?gag?ccg?tcg?aga?tgg?agt?tac?ctt?atg 2392
Met?Lys?Thr?Leu?Ile?Ala?Met?Glu?Pro?Ser?Arg?Trp?Ser?Tyr?Leu?Met
310 315 320
gat?ttc?tta?aga?gag?aaa?cgt?ata?ttg?gtc?act?gag?aat?tgg?gaa?gtt 2440
Asp?Phe?Leu?Arg?Glu?Lys?Arg?Ile?Leu?Val?Thr?Glu?Asn?Trp?Glu?Val
325 330 335
tca?ctg?ggt?tcc?atc?gag?tgc?atc?atg?cac?gac?gac?agc?att?agt?ttt 2488
Ser?Leu?Gly?Ser?Ile?Glu?Cys?Ile?Met?His?Asp?Asp?Ser?Ile?Ser?Phe
340 345 350
atg?atg?aat?ggc?cgg?gtc?gtt?ttc?aaa?ggg?caa?tct?gat?ggt?agg?tat 2536
Met?Met?Asn?Gly?Arg?Val?Val?Phe?Lys?Gly?Gln?Ser?Asp?Gly?Arg?Tyr
355 360 365 370
aac?cat?ggg?act?ggt?aca?gcc?gat?ctt?tgc?aca?gcg?att?act?ggt?gaa 2584
Asn?His?Gly?Thr?Gly?Thr?Ala?Asp?Leu?Cys?Thr?Ala?Ile?Thr?Gly?Glu
375 380 385
gct?aca?gat?atc?gtt?gat?tgt?gcc?atc?tac?cga?gag?gtc?cct?tgc?aat 2632
Ala?Thr?Asp?Ile?Val?Asp?Cys?Ala?Ile?Tyr?Arg?Glu?Val?Pro?Cys?Asn
390 395 400
tct?cca?acg?cac?gct?gag?ttg?ctt?gga?atg?tat?gtt?cta?gaa?aga?agg 2680
Ser?Pro?Thr?His?Ala?Glu?Leu?Leu?Gly?Met?Tyr?Val?Leu?Glu?Arg?Arg
405 410 415
gca?ata?tcg?ctg?aaa?ata?ctg?ttg?ttt?gat?gtg?aaa?acc?gac?aac?gct 2728
Ala?Ile?Ser?Leu?Lys?Ile?Leu?Leu?Phe?Asp?Val?Lys?Thr?Asp?Asn?Ala
420 425 430
ttt?gtc?agt?gaa?aca?gtc?aga?gat?atg?ttt?cct?att?act?cca?aac?act 2776
Phe?Val?Ser?Glu?Thr?Val?Arg?Asp?Met?Phe?Pro?Ile?Thr?Pro?Asn?Thr
435 440 445 450
act?gag?aag?gat?ctg?tgc?caa?gtg?ttg?aga?agt?atg?aaa?gtc?tat?ttc 2824
Thr?Glu?Lys?Asp?Leu?Cys?Gln?Val?Leu?Arg?Ser?Met?Lys?Val?Tyr?Phe
455 460 465
gaa?cac?ttc?aat?tgt?cgg?tgt?gaa?cct?cgg?gag?aaa?ctt?gag?cta?gtg 2872
Glu?His?Phe?Asn?Cys?Arg?Cys?Glu?Pro?Arg?Glu?Lys?Leu?Glu?Leu?Val
470 475 480
gat?agt?tta?atg?aaa?atg?aag?gat?aat?gag?atg?aca?atg?gaa?tca?atc 2920
Asp?Ser?Leu?Met?Lys?Met?Lys?Asp?Asn?Glu?Met?Thr?Met?Glu?Ser?Ile
485 490 495
aag?gat?aaa?tgg?gct?cat?tac?ctt?ctg?aga?ttg?cct?gtc?ttc?agg?gct 2968
Lys?Asp?Lys?Trp?Ala?His?Tyr?Leu?Leu?Arg?Leu?Pro?Val?Phe?Arg?Ala
500 505 510
cat?cag?cca?acc?aag?acc?ata?aga?aag?gac?tat?ata?a?gtaagtagtt 3015
His?Gln?Pro?Thr?Lys?Thr?Ile?Arg?Lys?Asp?Tyr?Ile
515 520 525
aatcttctga?gtacattgac?tttgattttt?ttttcttttt?tcttttaacc?catcggttta 3075
atttttatat?tgcag?ac aag?gct?cca?act?gtt?ggt?aca?gta?gct?ttc?cat 3125
Asn?Lys?Ala?Pro?Thr?Val?Gly?Thr?Val?Ala?Phe?His
530 535
ggg?caa?tac?aaa?att?gtc?gca?aag?ggg?gat?aca?gca?aaa?acg?gag?gct 3173
Gly?Gln?Tyr?Lys?Ile?Val?Ala?Lys?Gly?Asp?Thr?Ala?Lys?Thr?Glu?Ala
540 545 550
gtt?gtg?aac?ttg?gtg?cta?tcg?ctt?cag?cca?atg?gtt?gct?tca?gtt?ttc 3221
Val?Val?Asn?Leu?Val?Leu?Ser?Leu?Gln?Pro?Met?Val?Ala?Ser?Val?Phe
555 560 565 570
gtg?gga?aat?cac?atc?tca?gca?atc?tca?gtc?aag?gaa?gag?ata?gaa?aag 3269
Val?Gly?Asn?His?Ile?Ser?Ala?Ile?Ser?Val?Lys?Glu?Glu?Ile?Glu?Lys
575 580 585
ttt?gtc?aag?ggg?tat?tta?ctg?gac?atc?aag?gag?gct?aag?gga?gca?tat 3317
Phe?Val?Lys?Gly?Tyr?Leu?Leu?Asp?Ile?Lys?Glu?Ala?Lys?Gly?Ala?Tyr
590 595 600
gtt?gtt?gtc?atg?aaa?ttt?ata?gaa?act?ccc?aat?gca?ggt?ggt?aaa?ata 3365
Val?Val?Val?Met?Lys?Phe?Ile?Glu?Thr?Pro?Asn?Ala?Gly?Gly?Lys?Ile
605 610 615
tta?gat?gtc?ttg?ttt?aat?gct?gaa?gtc?ggt?gag?gtg?tac?aag?cag?aca 3413
Leu?Asp?Val?Leu?Phe?Asn?Ala?Glu?Val?Gly?Glu?Val?Tyr?Lys?Gln?Thr
620 625 630
gca?aac?tcg?gtc?tgc?gtc?aaa?cta?ctg?gac?aag?aat?gat?gga?act?act 3461
Ala?Asn?Ser?Val?Cys?Val?Lys?Leu?Leu?Asp?Lys?Asn?Asp?Gly?Thr?Thr
635 640 645 650
cca?gag?cta?agt?cca?gca?tgt?ctt?ctt?att?ttc?ggt?taagtcacaa 3507
Pro?Glu?Leu?Ser?Pro?Ala?Cys?Leu?Leu?Ile?Phe?Gly
655 660
agggtcaaac?tagtagtttc?ttggcatgtg?ccttctgtaa?gtgtgctgtt?taggtggctg 3567
gagggccctt?tttggttagt?agtctagacc?ctaacaacat?gttattactt?atgctgctgt 3627
tatgcaccct?gtgcagcacg?cttctgaatt?gcaacttctt?ttcaagctga?atttatctgc 3687
gagtgttaac?cctattttgt?gatggtcata?tatgtttatg?gatggagctt?caagctgggg 3747
catctgcagg?gatctgagta?ccagaactga?agttctttgt?gaaagaagtt?gttttgcttg 3807
tagatggcaa?tttccatgca?gtctcatctg?tacaaccccc?aaaaagcatc?attctgtttc 3867
agctacgagc?tctgtgtttg?tgcttttgtt?tttcttcttg?tttaccccag?gatgggatgg 3927
caatggtagg?gaatgctggt?gtatctagac?cgtgtttgtg?cttttgtttt?tcttcttgtt 3987
taccccagga?tgggacggca?atggtaggga?atgctggtgt?atctagacca?aggtttacaa 4047
actgcacggt?gatcacgcag?ttatcgtgat?tatcacgtgc?aacaaccttc?ttaaccttgt 4107
ggttttcatg?ataaccgtga?taaccatcaa?acggtgaggt?ggtggtaacc?caccccaaac 4167
gattcggtga?accttgatct?agacaggtga?tct 4200
<210>6
<211>662
<212>PRT
<213〉the Oryza rice strain 02428
<400>6
Met?Val?Met?Gly?Cys?Thr?His?Gln?Gln?Leu?Ala?Asp?Pro?Trp?Ala?Trp
1 5 10 15
Ala?Ala?Arg?Glu?Glu?Gly?Gly?Arg?Val?Ala?Gly?Ser?Ser?Leu?Pro?Arg
20 25 30
Arg?Pro?Thr?Pro?Thr?Thr?Ala?Ile?Gly?Arg?Val?Ala?Ala?Gly?Arg?Arg
35 40 45
Pro?Pro?Ala?Pro?Ala?Thr?Gly?Pro?Pro?Gln?Gly?Thr?Pro?Ala?Met?Ser
50 55 60
Leu?Ala?Pro?Pro?Pro?Pro?Lys?Ala?Ser?Pro?Ser?Ala?Ala?Arg?Leu?Gln
65 70 75 80
Lys?Pro?Ala?Ala?Ser?Cys?His?Gly?Gly?Gln?Pro?Lys?Ala?Ser?Pro?Trp
85 90 95
Ala?Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His?Leu?Gln?Gln?Pro?Ser?Arg
100 105 110
Met?Lys?Leu?Ser?Pro?Ser?Pro?Ala?Pro?Gly?Ala?Gln?His?Leu?Pro?Pro
115 120 125
Ala?Lys?Pro?Ser?Pro?Leu?Ala?Gly?Ser?Pro?Pro?Gly?Ala?Ala?Thr?His
130 135 140
Leu?Gln?Gln?Pro?Ala?Ala?Arg?Met?Leu?Ala?Ser?Ser?Pro?Ala?Lys?Arg
145 150 155 160
Val?Leu?Ser?Val?Phe?Ser?Pro?Ser?Ala?Ala?Gly?Ser?Leu?Arg?Arg?Glu
165 170 175
Arg?Asn?His?Leu?Pro?Pro?Leu?Ala?Ser?Cys?Phe?Cys?Leu?Arg?Arg?Leu
180 185 190
Met?Ser?Thr?Gly?Leu?Pro?Gln?Ile?Pro?Ser?Pro?Pro?Ala?Thr?Leu?Met
195 200 205
Arg?Pro?Trp?Cys?Asn?Glu?Asp?Glu?Gln?Val?Pro?Gln?Leu?Thr?Pro?Phe
210 215 220
Lys?Ile?Ser?His?Gly?Val?Ser?Asp?Thr?Met?Met?Lys?Gln?Tyr?Glu?Glu
225 230 235 240
Asp?Tyr?Lys?Tyr?Leu?Val?Glu?Glu?Gly?Leu?Asp?Leu?Glu?Glu?Met?Lys
245 250 255
Lys?Leu?Tyr?His?Glu?Leu?Val?Arg?Pro?Val?Leu?Val?Ala?Ser?Cys?Leu
260 265 270
Ile?Leu?Glu?His?His?Ile?Val?Ser?Pro?Glu?Thr?Lys?Ala?Leu?Asp?Gln
275 280 285
Lys?Ser?Lys?Asp?Lys?Phe?Gly?Lys?Pro?Pro?Ile?Tyr?Leu?Ser?Ala?Leu
290 295 300
Asp?Ile?Met?Lys?Thr?Leu?Ile?Ala?Met?Glu?Pro?Ser?Arg?Trp?Ser?Tyr
305 310 315 320
Leu?Met?Asp?Phe?Leu?Arg?Glu?Lys?Arg?Ile?Leu?Val?Thr?Glu?Asn?Trp
325 330 335
Glu?Val?Ser?Leu?Gly?Ser?Ile?Glu?Cys?Ile?Met?His?Asp?Asp?Ser?Ile
340 345 350
Ser?Phe?Met?Met?Asn?Gly?Arg?Val?Val?Phe?Lys?Gly?Gln?Ser?Asp?Gly
355 360 365
Arg?Tyr?Asn?His?Gly?Thr?Gly?Thr?Ala?Asp?Leu?Cys?Thr?Ala?Ile?Thr
370 375 380
Gly?Glu?Ala?Thr?Asp?Ile?Val?Asp?Cys?Ala?Ile?Tyr?Arg?Glu?Val?Pro
385 390 395 400
Cys?Asn?Ser?Pro?Thr?His?Ala?Glu?Leu?Leu?Gly?Met?Tyr?Val?Leu?Glu
405 410 415
Arg?Arg?Ala?Ile?Ser?Leu?Lys?Ile?Leu?Leu?Phe?Asp?Val?Lys?Thr?Asp
420 425 430
Asn?Ala?Phe?Val?Ser?Glu?Thr?Val?Arg?Asp?Met?Phe?Pro?Ile?Thr?Pro
435 440 445
Asn?Thr?Thr?Glu?Lys?Asp?Leu?Cys?Gln?Val?Leu?Arg?Ser?Met?Lys?Val
450 455 460
Tyr?Phe?Glu?His?Phe?Asn?Cys?Arg?Cys?Glu?Pro?Arg?Glu?Lys?Leu?Glu
465 470 475 480
Leu?Val?Asp?Ser?Leu?Met?Lys?Met?Lys?Asp?Asn?Glu?MetThr?Met?Glu
485 490 495
Ser?Ile?Lys?Asp?Lys?Trp?Ala?His?Tyr?Leu?Leu?Arg?Leu?Pro?Val?Phe
500 505 510
Arg?Ala?His?Gln?Pro?Thr?Lys?Thr?Ile?Arg?Lys?Asp?Tyr?Ile?Asn?Lys
515 520 525
Ala?Pro?Thr?Val?Gly?Thr?Val?Ala?Phe?His?Gly?Gln?Tyr?Lys?Ile?Val
530 535 540
Ala?Lys?Gly?Asp?Thr?Ala?Lys?Thr?Glu?Ala?Val?Val?Asn?Leu?Val?Leu
545 550 555 560
Ser?Leu?Gln?Pro?Met?Val?Ala?Ser?Val?Phe?Val?Gly?Asn?His?Ile?Ser
565 570 575
Ala?Ile?Ser?Val?Lys?Glu?Glu?Ile?Glu?Lys?Phe?Val?Lys?Gly?Tyr?Leu
580 585 590
Leu?Asp?Ile?Lys?Glu?Ala?Lys?Gly?Ala?Tyr?Val?Val?Val?Met?Lys?Phe
595 600 605
Ile?Glu?Thr?Pro?Asn?Ala?Gly?Gly?Lys?Ile?Leu?Asp?Val?Leu?Phe?Asn
610 615 620
Ala?Glu?Val?Gly?Glu?Val?Tyr?Lys?Gln?Thr?Ala?Asn?Ser?Val?Cys?Val
625 630 635 640
Lys?Leu?Leu?Asp?Lys?Asn?Asp?Gly?Thr?Thr?Pro?Glu?Leu?Ser?Pro?Ala
645 650 655
Cys?Leu?Leu?Ile?Phe?Gly
660
Claims (7)
1, a kind of paddy rice hybrid fertility gene, the sequence of its proteins encoded is: SEQ ID NO:2 or SEQ ID NO:4 or SEQ ID NO:6.
2, gene according to claim 1, its nucleotides sequence are classified SEQ ID NO:1 or SEQ ID NO:3 or SEQ ID NO:5 as.
3, a kind of carrier that contains claim 1 or 2 described genes.
4, a kind of by the described carrier transformed host cells of claim 3.
5. a method of cultivating the affine system of paddy rice hybrid fertility is characterized in that: with claim 1 or 2 described genes or the described carrier rice transformation of claim 3, obtain the affine system of transgenic paddy rice hybrid fertile; Or select to cultivate the affine system of paddy rice hybrid fertility of containing claim 1 or 2 described genes with hybridizing-backcross method and molecule marker.
6. molecule marker or its close linkage mark that produces according to the nucleotide sequence of claim 1 or 2 described genes.
7. the application of the described molecule marker of claim 6 in the paddy rice cross breeding breeding.
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CN100457905C true CN100457905C (en) | 2009-02-04 |
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CN114085847B (en) * | 2021-12-22 | 2022-08-30 | 云南农业大学 | Rice OsSUT3 gene mutant and molecular identification method and application thereof |
CN116606916B (en) * | 2023-06-30 | 2023-11-28 | 河北师范大学 | Method for identifying, screening or controlling wheat spike number per spike based on SNP locus |
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CN1401219A (en) * | 2002-04-27 | 2003-03-12 | 华南农业大学 | Method for breeding light- and temp.-sensitive nucleus sterile line rice with plant age regulatory fertility |
US20050048482A1 (en) * | 2000-08-17 | 2005-03-03 | Toshiyuki Komori | Method of estimating the genotype of fertility recovery locus to rice bt type male fertility cytoplasm |
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US20050048482A1 (en) * | 2000-08-17 | 2005-03-03 | Toshiyuki Komori | Method of estimating the genotype of fertility recovery locus to rice bt type male fertility cytoplasm |
CN1401219A (en) * | 2002-04-27 | 2003-03-12 | 华南农业大学 | Method for breeding light- and temp.-sensitive nucleus sterile line rice with plant age regulatory fertility |
Non-Patent Citations (2)
Title |
---|
用RAPD标记对栽培稻F1花粉不育基因座S-b定位. 庄楚雄等.遗传学报,第29卷第8期. 2002 |
用RAPD标记对栽培稻F1花粉不育基因座S-b定位. 庄楚雄等.遗传学报,第29卷第8期. 2002 * |
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