CN116725015A - Application of exogenous choline aspartic acid in improving corn yield - Google Patents
Application of exogenous choline aspartic acid in improving corn yield Download PDFInfo
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- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 title claims abstract description 103
- 235000003704 aspartic acid Nutrition 0.000 title claims abstract description 91
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 title claims abstract description 91
- 229960001231 choline Drugs 0.000 title claims abstract description 86
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 title claims abstract description 83
- 240000008042 Zea mays Species 0.000 title claims abstract description 58
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 58
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 54
- 235000005822 corn Nutrition 0.000 title claims abstract description 54
- HMBHAQMOBKLWRX-UHFFFAOYSA-N 2,3-dihydro-1,4-benzodioxine-3-carboxylic acid Chemical compound C1=CC=C2OC(C(=O)O)COC2=C1 HMBHAQMOBKLWRX-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229940075419 choline hydroxide Drugs 0.000 claims abstract description 25
- 239000007864 aqueous solution Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 11
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- 239000007952 growth promoter Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 13
- 230000001965 increasing effect Effects 0.000 claims description 11
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- 241001057636 Dracaena deremensis Species 0.000 claims description 4
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- 150000001875 compounds Chemical class 0.000 abstract description 10
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- 229940009098 aspartate Drugs 0.000 description 8
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 6
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- YNWVFADWVLCOPU-MDWZMJQESA-N (1E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol Chemical compound C1=NC=NN1/C(C(O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1 YNWVFADWVLCOPU-MDWZMJQESA-N 0.000 description 1
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 1
- PWVXXGRKLHYWKM-UHFFFAOYSA-N 5-[2-(benzenesulfonyl)ethyl]-3-[(1-methylpyrrolidin-2-yl)methyl]-1h-indole Chemical compound CN1CCCC1CC(C1=C2)=CNC1=CC=C2CCS(=O)(=O)C1=CC=CC=C1 PWVXXGRKLHYWKM-UHFFFAOYSA-N 0.000 description 1
- 239000005985 Paclobutrazol Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
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- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
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- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 230000002786 root growth Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C213/00—Preparation of compounds containing amino and hydroxy, amino and etherified hydroxy or amino and esterified hydroxy groups bound to the same carbon skeleton
- C07C213/08—Preparation of compounds containing amino and hydroxy, amino and etherified hydroxy or amino and esterified hydroxy groups bound to the same carbon skeleton by reactions not involving the formation of amino groups, hydroxy groups or etherified or esterified hydroxy groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/14—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof
- C07C227/18—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof by reactions involving amino or carboxyl groups, e.g. hydrolysis of esters or amides, by formation of halides, salts or esters
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application belongs to the technical field of plant growth promoters, and particularly relates to application of exogenous choline aspartic acid in improving corn yield, wherein choline aspartic acid is prepared according to the following steps: mixing the aqueous solution of the choline hydroxide with the aspartic acid at normal temperature, then carrying out shaking table reaction for 48-72h at the rotating speed of 180-220r/min, and removing water after the reaction is completed to obtain choline aspartic acid; the application explores the application of exogenous choline aspartic acid as a growth promoter or a synergist in improving the corn yield, and the application uses the choline aspartic acid which is synthesized autonomously as a material, and discovers that the compound is sprayed in the early stage of corn grouting, so that the increase of the corn cob grain weight can be obviously promoted, and the purposes of promoting the corn growth and improving the corn yield are achieved.
Description
Technical Field
The application belongs to the technical field of plant growth promoters, and particularly relates to application of exogenous choline aspartic acid in improving corn yield.
Background
Choline is a small molecular substance widely existing in animal and plant cells, and participates in the construction of biological cell membranes and other structures, and is also an important micronutrient. Choline is nontoxic and biodegradable, and is an excellent cation selector for synthesizing green compounds.
Corn is widely planted worldwide as an important grain and feed crop. In recent years, with the development of related industries such as animal husbandry and processing industry, the demand of corn as main feed and industrial raw materials is continuously increasing. Drought and water shortage are main limiting factors for restricting the production of corns in China, and trigger different changes of crops through various reactions such as morphology, physiology, biochemistry and the like, so that metabolism and physiology of the corns are damaged, and the yield level is reduced. The reasonable use of exogenous plant growth substances is an important means for regulating and improving the growth and development conditions of crops and improving stress resistance.
At present, although various chemical regulating agents such as jade gold, chlormequat chloride, uniconazole (paclobutrazol) and the like commonly exist in the corn production, the agents have a relatively common problem in the use process, namely the agents are mostly growth inhibition type plant growth regulators, and the action mechanism of the agents is generally to control vigorous growth or overgrowth of plants, promote root growth and seedling strengthening, properly reduce plant height, increase stem thickness, improve lodging resistance and yield and the like. Therefore, the method is more suitable for controlling the plant vigorous under the condition of good water and fertilizer conditions, prevents greedy, overgrowth, lodging and the like, and is not fully suitable under the conditions of poor production environment such as drought, barren soil and the like. Moreover, the use of the compound has relatively high technical requirements on operators, if the concentration is too high or too low, the use period is incorrect, and the like, so that the compound cannot normally function in actual use, even plays an opposite role, seriously hurts the use enthusiasm of manufacturers, and limits the application of the regulator.
Disclosure of Invention
Aiming at the defects of the prior art, the application aims to provide the application of exogenous choline aspartic acid in improving the corn yield, and the application of exogenous choline aspartic acid as a growth promoter or synergist in improving the corn yield is explored by taking the problems that the effect is not obvious, the technical requirement is higher and the like possibly existing in the use process of a growth inhibition type regulator in the current corn production into consideration. The application uses choline aspartic acid which is synthesized autonomously as a material, and discovers that the compound is sprayed in the early stage of corn grouting, so that the increase of corn ear grain weight can be obviously promoted, and the purposes of promoting corn growth and improving corn yield are achieved.
In order to solve the technical problems, the application adopts the following technical scheme:
use of exogenous choline aspartic acid in increasing corn yield, wherein the choline aspartic acid has a structure as shown in formula (I):
preferably, the choline aspartic acid is prepared according to the following steps:
mixing the aqueous solution of the choline hydroxide with the aspartic acid at normal temperature, and then carrying out shaking table reaction for 48-72h to remove water to obtain choline aspartic acid.
Preferably, the molar ratio of choline hydroxide to aspartic acid is 1:1.
Preferably, the mixing mode is as follows: aspartic acid was added dropwise to the aqueous choline hydroxide solution with stirring and mixed.
Preferably, the rotation speed of the shaking table is 180-220r/min.
Preferably, the choline aspartic acid is used for preparing a corn growth promoter or synergist.
Preferably, the application method comprises the following steps: in the early stage of corn grouting, choline aspartic acid is used for carrying out spraying treatment on corn plants, the spraying concentration is 50-100mg/L, and the spraying dosage is 20-40L/mu.
Compared with the prior art, the application has the following beneficial effects:
1. choline aspartic acid ([ Chl ] [ Asp ]) is a novel choline compound synthesized by taking choline as a cation and aspartic acid as an anion, and the promotion effect of the choline compound on crop yield is not reported before. The research adopts a foliar spraying method, the effect of adding choline aspartic acid with low concentration (50-100 mg/L) in corn cultivation is examined for the first time, and the result shows that choline aspartic acid can effectively promote the increase of the grain weight of the ear and improve the yield, and the research provides a new technical means for the development and the application of choline aspartic acid as a corn yield-increasing promoter.
2. The action mechanism of choline aspartic acid as an accelerator or synergist is as follows: through our study, the promotion effect of choline aspartic acid spraying on corn yield may have the following two mechanisms:
(1) Choline aspartic acid improves the nutrition level of corn in the form of a micro-fertilizer, promotes the accumulation of plant biomass, and further improves the grain weight and yield of the corn;
(2) The research of the application shows that exogenous addition of choline aspartic acid can significantly improve the photosynthesis of corn leaves, so that the exogenous addition of choline aspartic acid can improve the corn yield by increasing the photosynthesis capacity.
3. The choline aspartic acid synthesized by the research aims to effectively combine the physiological functions of choline and aspartic acid, and the action mechanism of the choline aspartic acid is different from that of a growth inhibition type regulator, and the choline aspartic acid is used as a trace fertilizer or a physiologically active substance, so that the biomass and the yield of a single plant are improved by directly improving the growth condition of the plant, and the problems that the growth inhibition type regulator may have poor actual use effect, higher technical requirements and the like are avoided from the action mechanism. Meanwhile, the synthesis method of choline aspartic acid is simple and convenient to operate, the used reagents are common reagents which are easy to obtain, the synthesis and preparation cost is low, raw materials and products choline aspartic acid used in the synthesis process are green compounds with extremely low toxicity, no by-product is produced in the synthesis process, and the synthesis method has the characteristic of environmental friendliness. Therefore, the compound can provide technical support for the development and application of novel efficient corn yield promoters.
Drawings
FIG. 1 is a physical diagram of choline aspartic acid obtained in example 1 of the present application;
FIG. 2 is a hydrogen spectrum of choline aspartic acid obtained in example 1 of the present application;
FIG. 3 is a diagram of corn plants in the experimental group and the control group according to the application.
Detailed Description
The following detailed description of specific embodiments of the application is, but it should be understood that the application is not limited to specific embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application. The experimental methods described in the examples of the present application are conventional methods unless otherwise specified.
Example 1
The preparation method of choline aspartic acid comprises the following steps:
choline aspartic acid is synthesized by using two compounds of choline hydroxide aqueous solution and aspartic acid, wherein the volume ratio of choline hydroxide to water in the choline hydroxide aqueous solution is 46:100, the synthesis method is mainly middle legal, and comprises the following specific steps:
mixing the aqueous solution of choline hydroxide with aspartic acid according to a molar ratio of 1:1 at normal temperature, dropwise adding and mixing the aqueous solution of choline hydroxide with aspartic acid in a gradually small amount, dropwise adding the aspartic acid into the aqueous solution of choline hydroxide, performing the whole process on a magnetic stirrer, then reacting for 48 hours on a shaking table, wherein the temperature of the shaking table is 18 ℃, the rotating speed is 200r/min, and removing water at 55 ℃ by using a rotary evaporator after the reaction is completed, thus obtaining choline aspartic acid.
Example 2
The preparation method of choline aspartic acid comprises the following steps:
choline aspartic acid is synthesized by using two compounds of choline hydroxide aqueous solution and aspartic acid, wherein the volume ratio of choline hydroxide to water in the choline hydroxide aqueous solution is 40:100, the synthesis method is mainly middle legal, and comprises the following specific steps:
mixing the aqueous solution of choline hydroxide with aspartic acid according to a molar ratio of 1:1 at normal temperature, dropwise adding and mixing the aqueous solution of choline hydroxide in a gradually small amount, dropwise adding the aspartic acid into the aqueous solution of choline hydroxide, performing the whole process on a magnetic stirrer, then reacting for 72 hours on a shaking table at a temperature of 25 ℃ at a rotating speed of 220r/min, and removing water at a temperature of 55 ℃ after the reaction is completed by using a rotary evaporator to obtain choline aspartic acid.
Example 3
The preparation method of choline aspartic acid comprises the following steps:
choline aspartic acid is synthesized by using two compounds of choline hydroxide aqueous solution and aspartic acid, wherein the volume ratio of choline hydroxide to water in the choline hydroxide aqueous solution is 50:100, the synthesis method is mainly middle legal, and comprises the following specific steps:
mixing the aqueous solution of choline hydroxide with aspartic acid according to a molar ratio of 1:1 at normal temperature, dropwise adding and mixing the aqueous solution of choline hydroxide with aspartic acid in a gradually small amount, dropwise adding the aspartic acid into the aqueous solution of choline hydroxide, performing the whole process on a magnetic stirrer, then reacting for 60 hours on a shaking table, wherein the temperature of the shaking table is 35 ℃, the rotating speed is 180r/min, and removing water at 55 ℃ by using a rotary evaporator after the reaction is completed, thus obtaining choline aspartic acid.
1. Structural analysis
The choline aspartic acid of examples 1-3 of the present application was prepared in high purity, and the choline aspartic acid yields were all greater than 95%, and were liquid at room temperature, light yellow to light brown (fig. 1). The nuclear magnetic resonance spectrum is adopted to carry out structural identification on choline aspartic acid prepared in the example 1 (figure 2), and the purity of the synthesized choline aspartic acid is higher than 95% as can be seen from the integral proportion of hydrogen atoms in the hydrogen spectrum, so that the structural correctness of synthesis is verified.
TABLE 1 choline aspartic acid nuclear magnetic resonance spectrum identification results
2. Analysis of the Effect of choline aspartic acid spray on corn yield
(1) The research method comprises the following steps:
the test was conducted for two years, 6-10 months 2021 and 6-10 months 2022, respectively, on a Cao Xinzhuang test farm from the university of agriculture and forestry, northwest, located in Yang Ling demonstration area of salty yang, shaanxi. The basic nutrients of the soil in the test land are as follows: soil pH 8.26, organic matter 15.98g/kg, quick-acting phosphorus 28.67mg/kg, quick-acting potassium 170mg/kg, total nitrogen 1.01g/kg, and test material of corn Zhengdan 958 (Beijing De agricultural industry).
Spraying conditions: the corn plants are sprayed with exogenous choline aspartic acid at the early stage of corn grouting (experimental group), the spraying concentration is 100mg/L, the spraying dosage is 30L/mu, clear water is sprayed as a control group (CK), and the cell area is 27m 2 And 3 times of repetition are carried out each time of treatment, the planting density is 4500 plants/mu, the conventional field management is carried out, the cells are arranged randomly, and the management measures are consistent. Sampling in the mature period of corn, oven drying to measure biomass of each part, and measuring indexes such as spike weight by using a weighing method.
(2) Study results:
tables 2 and 3 show the effect of Ji Waiyuan choline aspartate on corn yield and biomass for two year 2021-2022 corn growth. Compared with the control, in 2021, the biomass of the corn single plant treated by choline aspartic acid ([ Chl ] [ Asp ]) is improved by 14.8%, the grain weight of the corn single plant is improved by 13.3%, and the yield is improved by 14%. In 2022, the biomass of corn single plant treated by choline aspartic acid ([ Chl ] [ Asp) is improved by 15.6%, the grain weight is improved by 15.5%, and the yield is improved by 15.3%. The result shows that the exogenous choline aspartic acid treatment is beneficial to improving the corn ear grain weight and increasing the yield, and simultaneously compared with the control stem biomass, the leaf biomass and the like, the choline aspartic acid treatment obviously increases, so that the yield increasing action mechanism of the choline aspartic acid can be related to promoting the growth of plant stems, leaves and the like, improving the plant single plant biomass, further improving the corn ear grain weight, the yield and the like.
TABLE 2 influence of exogenous choline aspartate on corn biomass for 2021-2022 years
Note that: data in the tables are presented as mean ± standard deviation (n=3), "x" indicates that there was a significant difference (p < 0.05) between the experimental and control groups. The following is the same.
TABLE 3 influence of exogenous choline aspartic acid on ear grain weight and yield in 2021-2022 years
The physical diagram is shown in FIG. 3, and is consistent with the conclusions in tables 2 and 3.
(3) Analysis of physiological mechanisms
To examine the molecular mechanism of choline aspartic acid to regulate corn growth, we performed transcriptome differential gene expression analysis on exogenously added choline aspartic acid ([ Chl ] [ Asp ]) leaves and control (no addition) leaves using the method of hydroponic nutrient addition. The results show that the addition of low concentration choline aspartate (20 mg/L) to the hydroponic nutrient solution caused a significant change in the expression profile of the genes associated with maize leaf photosynthesis and chlorophyll synthesis compared to the leaves of the control (CK, no choline aspartate).
The results of the studies are shown in tables 4 and 5, and in the chlorophyll metabolic pathway, compared with the control, the expression of all 15 genes related to chlorophyll synthesis under the treatment of choline aspartic acid is significantly up-regulated, and the choline aspartic acid significantly promotes the chlorophyll anabolic pathway (table 4). In the photosynthesis pathway, compared with the control, 15 out of the 17 differential genes in choline aspartate treatment were up-regulated and only 2 were down-regulated, wherein most of the genes encoding the photosystem response center protein were up-regulated, and it was seen that choline aspartate had a significant promotion effect on the expression of the genes related to photosynthesis (table 5). Based on the above analysis, the mechanism of action of exogenous choline amino acids on regulation of maize growth may be related to its promotion of photosynthesis and chlorophyll synthesis pathways.
TABLE 4 differential expression of genes for chlorophyll metabolic pathways in maize leaves for choline aspartate treatment compared to controls
TABLE 5 differential expression of genes for maize leaf photosynthesis pathways for choline aspartate treatment compared to control
Various modifications and alterations of this application may be made by those skilled in the art without departing from the spirit and scope of this application. Thus, it is intended that the present application also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (7)
1. The application of exogenous choline aspartic acid in improving corn yield is characterized in that the choline aspartic acid has a structure shown in a formula (I):
2. use of exogenous choline aspartic acid according to claim 1 for increasing corn yield, wherein the choline aspartic acid is prepared according to the following steps:
mixing the aqueous solution of the choline hydroxide with the aspartic acid at normal temperature, and then carrying out shaking table reaction for 48-72h to remove water to obtain choline aspartic acid.
3. Use of exogenous choline aspartic acid according to claim 2 for increasing corn yield, wherein the molar ratio of choline hydroxide to aspartic acid is 1:1.
4. Use of exogenous choline aspartic acid according to claim 2 for increasing corn yield, wherein the mixing is by: aspartic acid was added dropwise to the aqueous choline hydroxide solution with stirring and mixed.
5. The use of exogenous choline aspartic acid according to claim 2 for increasing corn yield, wherein the rotation speed of the shaker is 180-220r/min.
6. The use of exogenous choline aspartic acid according to claim 1 for increasing corn yield, wherein the choline aspartic acid is used to prepare a corn growth promoter or booster.
7. The use of exogenous choline aspartic acid according to claim 6 for increasing corn yield, wherein the use method is: in the early stage of corn grouting, choline aspartic acid is used for carrying out spraying treatment on corn plants, the spraying concentration is 50-100mg/L, and the spraying dosage is 20-40L/mu.
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