CN116724871A - Method for improving seedling quality of aromatic tree - Google Patents

Method for improving seedling quality of aromatic tree Download PDF

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Publication number
CN116724871A
CN116724871A CN202310922321.6A CN202310922321A CN116724871A CN 116724871 A CN116724871 A CN 116724871A CN 202310922321 A CN202310922321 A CN 202310922321A CN 116724871 A CN116724871 A CN 116724871A
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China
Prior art keywords
seedlings
shaking
tree
culture
quality
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CN202310922321.6A
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Chinese (zh)
Inventor
郝向春
周帅
徐瑢
韩丽君
栗永红
孙剑
陈天成
翟瑜
陈思
任达
段福军
吴建华
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Shanxi Academy Of Forestry And Grassland Sciences
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Shanxi Academy Of Forestry And Grassland Sciences
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Priority to CN202310922321.6A priority Critical patent/CN116724871A/en
Publication of CN116724871A publication Critical patent/CN116724871A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a method for improving the seedling quality of a tree. Belonging to the technical field of plant cultivation. According to the method, greenhouse culture and shaking culture are combined, biomass distribution in the growth process of the seedlings of the aromatic tree is effectively regulated and controlled, the ratio of ground diameter to plant height is remarkably improved, the ratio of the thickness of stems to the underground biomass is improved, the effects of strengthening seedlings and improving the quality of seedlings are achieved, and technical support is provided for propagation and large-scale utilization of the population of the aromatic tree.

Description

Method for improving seedling quality of aromatic tree
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for improving the seedling raising quality of a strand-shaped tree.
Background
The Litsea coreana (Cercidiphyllum japonicum) is a deciduous tree of Litsea of Litseae, and is mainly distributed in southwest part of Shanxi, henan, shaanxi, gansu, anhui, zhejiang, jiangxi, hubei and Sichuan of China. The aromatic tree is a single plant of the wiggles of the third-stage ancient tropical plant and is an ancient original woody plant. The tree has unique taxonomic status in plant groups, and can provide important scientific value for the origin and phylogenetic development of the belonging plants. The fructus Litseae can be used as medicine, bark and leaf can be used for preparing tannin extract, and leaf can be used for preparing flavoring enhancer. In addition, the wood grain of the continuous aromatic tree is straight, the structure is fine, the continuous aromatic tree is not only a rare and precious wood species, but also an important coinage tree species, the tree pose is graceful, the leaf shape is peculiar, the Ji Xiangbian is rich, and the continuous aromatic tree has higher ornamental value in gardens.
The natural updating is difficult, the resources are rare, the natural updating is endangered, the natural updating is listed in Chinese rare endangered plant directory, chinese plant red book and national important protection wild plant directory, and the natural updating is a national second important protection wild plant species. For many years, scientific researchers try to artificially propagate the aromatic tree in various modes such as cuttage, tissue culture, seed sowing and the like, but due to fewer nutrient substances carried by seeds and severe requirements of seedlings on the cultivation habitat conditions, the nursery yield of the seedlings is lower, and the nursery quality is poor. Therefore, strong seedling cultivation becomes a technical bottleneck for propagation and scale utilization of the strand of the tree.
At the present stage, the seedling strengthening technology of the strand-leaf tree is mainly based on methods of fertilizing and strengthening seedlings, adjusting growth hormone and spraying biological agents to strengthen seedlings, but long-term excessive fertilization can cause soil acidification, salinization and unbalance of soil microorganisms; improper use of auxins can affect the growth and development of the strand, even resulting in malformation of plants; biological agents are of a wide variety, and improper use of the type and concentration of the agent can cause poor plant growth or adverse effects. In addition, the reagents used in these techniques inevitably pollute the environment and increase carbon emissions during the preparation and use.
Therefore, how to provide a safer, efficient and environment-friendly method for improving the seedling quality of the tree, which is a technical problem to be solved by the technicians in the field.
Disclosure of Invention
In view of the above, the invention provides a method for improving the seedling raising quality of the aromatic tree, which combines greenhouse culture and shaking culture, effectively regulates and controls biomass distribution in the growth process of the aromatic tree seedling, obviously improves the ratio of ground diameter to plant height, improves the stem robustness and the ratio of underground biomass, and achieves the effects of strengthening seedlings and improving the quality of the seedlings.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for improving the quality of seedlings of Litsea Coreana comprises placing the seedlings of Litsea Coreana on a shaking table, shaking at uniform speed, and culturing to obtain high-quality seedlings of Litsea Coreana.
The method for improving the seedling quality of the strand-shaped tree comprises the following steps:
(1) Matrix pretreatment and seed sowing: sterilizing the matrix for raising seedlings of the tree, filling the matrix into a seedling raising container, watering fully, and sowing into germinated seeds;
(2) Seed pre-culture: placing the sown seedling raising container in a greenhouse, and culturing under full illumination until the leaves of the seedlings grow to 4-5 pairs to obtain pre-cultured seedlings;
(3) Culturing seedlings by shaking: the culture conditions are kept unchanged, the pre-cultured seedlings are placed on the shaking table after sunset, the seedlings are cultured in a shaking mode at a constant speed, and the shaking table is closed after sunset, so that the quality of the seedlings can be improved.
Preferably, the preparation method of the strongpoint-tree seedling substrate in the step (1) comprises the following steps: perlite, vermiculite, peat: the coconut husk is prepared by mixing according to the volume ratio of 1:1:2:2; the sowing depth of the seeds is 0.5-1mm.
Preferably, the greenhouse full-illumination culture conditions in the step (2) are as follows: the temperature in the daytime is 25-30 ℃, the air humidity is 50-80%, the temperature in the night is 15-18 ℃ and the air humidity is 50-80%.
Further preferably, the greenhouse full-illumination culture conditions in the step (2) are as follows: the temperature of the air is 28 ℃ in the daytime, the air humidity is 55%, the temperature of the air is 17 ℃ at night and the air humidity is 75%.
Preferably, the shaking culture time in the step (3) is as follows: 3-10 months, 5-6d per week, 8-10h per day; the shaking culture speed is as follows: 60-90rpm.
Further preferably, the shaking culture speed in the step (3) is: 80rpm.
Compared with the prior art, the invention has the following beneficial effects:
it is generally believed that the quality of seedlings grown in the field is better than that of seedlings grown in the greenhouse, particularly in terms of the degree of stem thickening, root system development, etc., which is largely caused by the lack of blowing of seedlings in the greenhouse. On the other hand, greenhouse seedling culture has great advantages in the aspects of growth environment control, seedling growth speed and the like, and is incomparable with field seedling culture. In view of the above, the invention combines greenhouse culture and shaking culture, not only can mechanically shake seedlings, but also can effectively regulate and control the seedling raising environment to avoid excessive water loss of the seedlings caused by excessive wind speed, and the like, thereby realizing effective regulation and control of biomass distribution in the growth of the seedlings of the Chinese parsley, remarkably improving the ratio of ground diameter to plant height, improving the thickness of stems and the occupation ratio of underground biomass, and achieving the effects of strengthening seedlings and improving the quality of the seedlings.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
Fig. 1 is a diagram of: a test group, a control group 1 and a control group 2 are arranged from left to right in sequence;
fig. 2 is a diagram of: test group, control group 1 and control group 2 are shown in this order from left to right.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Influence of night shaking on growth traits of seedlings of the Litsea japonica
Taking 100 seedlings (average plant height 12cm, average ground diameter 1.4mm, average 4 pairs of leaves for each plant) of the Litsea coreana with consistent growth vigor, placing the seedlings in a full-illumination greenhouse, and carrying out adaptive cultivation for 1 week, wherein the cultivation conditions of the full-illumination greenhouse are as follows: day 28 ℃, air humidity 55%; the night temperature is 17 ℃, and the air humidity is 75%. After the end of the acclimation, the seedlings were equally divided into test and control groups 1, and the conditions of the test and control groups 1 were as follows:
test group: after sunset, placing the seedlings of the Chinese juniper and the seedling raising container on a shaking seedling bed at 80rpm, shaking at a uniform speed, and closing a shaking table after the sun rises the next day;
control group 1: not placed on shake Miao Chuangshang at night;
the environmental condition settings of both groups during the trial were consistent with the acclimation period and the trial lasted 3 months. After the test is finished, 2 treated plant heights, ground diameters, the ratio of the ground diameters to the plant heights, the relative chlorophyll content of the leaves and the net photosynthetic rate are counted, the ratio of the underground biomass to the above-ground biomass after drying, the specific surface area and the strong seedling index are counted, and the experimental results are shown in tables 1-3 and figures 1-2.
TABLE 1 influence of night shaking on the growth traits of seedlings of Styrax
Height of plant (cm) Ground diameter (cm) Ground diameter/plant height
Test group 28.35±2.90 0.41±0.02 0.014±0.001
Control group 1 39.49±4.01* 0.42±0.02 0.011±0.001**
Note that: * Control group 1 significantly different from the test group, meaning control group 1 significantly different from the test group.
TABLE 2 influence of night shaking on photosynthetic traits of seedlings of Styrax
Leaf relative chlorophyll content Net photosynthetic rate (. Mu. Mol.m) -2 ·s -1 )
Test group 32.42±2.18 8.96±1.64
Control group 1 30.38±2.58 9.52±1.21
TABLE 3 influence of night shaking on the biomass index of seedlings of Styrax
Underground biomass/above-ground biomass Specific leaf area (cm) 2 ·mg -1 ) Index of strong seedlings
Test group 0.32±0.04 0.47±0.03 401.65±66.01
Control group 1 0.28±0.05** 0.46±0.03 427.69±141.69
Note that: * Control group 1 was shown to be very significantly different from the test group.
Analysis of results: the ratio of the ground diameter to the plant height of the seedlings of the Chinese juniper, the ratio of the underground biomass and other numerical values are obviously higher than those of a control group, and photosynthesis related indexes, leaf area and seedling strengthening indexes are not obviously different, so that the distribution of the biomass of the seedlings of the Chinese juniper can be influenced by shaking, and more energy is used for improving the thickness of stems and the biomass of the underground part.
Example 2
Influence of night shaking and wind blowing on growth traits of seedlings of the Litsea japonica
100 seedlings of the Litsea coreana (average plant height 12cm, average ground diameter 1.4mm, average 4 pairs of leaves for each plant) with consistent growth vigor are taken and placed in a full-illumination greenhouse for adaptive cultivation for 1 week. The culture conditions of the full-illumination greenhouse are as follows: day 28 ℃, air humidity 55%; the night temperature is 17 ℃, and the air humidity is 75%. After the end of the adaptation culture, seedlings were equally divided into 2 groups, the specific conditions were as follows:
test group: after sunset, the seedlings of the mangosteen and the seedling raising container are placed on a shaking seedling bed at 80rpm, the seedlings are shaken at a constant speed, and after sunrise is carried out the next day, the shaking table is closed.
Control group 2: the device is placed at night to shake Miao Chuangshang, and a fan is adopted to create a wind blowing effect, and the wind speed is set to be 3.0m/s.
The environmental condition settings of both groups during the trial were consistent with the acclimation period and the trial lasted 3 months. After the test, 2 treated plant heights, ground diameters, ratios of ground diameters to plant heights, relative chlorophyll content of leaves, net photosynthetic rate are counted, and the ratio of underground biomass to above-ground biomass, specific surface area and strong seedling index after drying are obtained, and experimental results are shown in tables 4-5 and attached figures 1-2.
TABLE 4 influence of night shaking and wind blowing on growth traits of seedlings of Litsea Coreana
Height of plant (cm) Ground diameter (cm) Ground diameter/plant height
Test group 28.35±2.90 0.41±0.02 0.014±0.002
Control group 2 23.38±2.77** 0.37±0.02** 0.014±0.002
Note that: * Control group 2 was shown to be very significantly different from the test group.
TABLE 5 influence of night shaking and wind blowing on photosynthetic traits of seedlings of Litsea Coreana
Leaf relative chlorophyll content Net photosynthetic rate (. Mu. Mol.m) -2 ·s -1 )
Test group 32.42±2.18 8.96±1.64
Control group 2 30.49±2.93 5.7±0.73**
Note that: * Control group 2 was shown to be very significantly different from the test group.
TABLE 6 influence of night shaking and wind blowing on the biomass index of seedlings of Litsea Coreana
Underground biomass/above-ground biomass Specific leaf area (cm) 2 ·mg -1 ) Index of strong seedlings
Test group 0.32±0.04 0.47±0.03 401.65±66.01
Control group 2 0.31±0.05 0.42±0.04** 285.01±88.16**
Note that: * Control group 2 was shown to be very significantly different from the test group.
Analysis of results: the method has the advantages that the method only adopts the siamesed tree seedlings which shake at night, and is superior to the treatment of shaking+wind blowing in the indexes of plant height, ground diameter, net photosynthetic rate, specific surface area, strong seedling index and the like, so that the growth quantity, photosynthetic rate and seedling quality of the siamesed tree seedlings can be improved only by adopting a shaking mode.
Example 3
Exploring the influence of different shaking speeds on the growth characteristics of the seedlings of the Litsea japonica
60 seedlings of the Litsea coreana (average plant height 12cm, average ground diameter 1.4mm, average 4 pairs of leaves for each plant) with consistent growth vigor are taken and placed in a full-illumination greenhouse for adaptive cultivation for 1 week. The culture conditions of the full-illumination greenhouse are as follows: day 28 ℃, air humidity 55%; the night temperature is 17 ℃, and the air humidity is 75%. After the end of the acclimatization, seedlings were equally divided into 3 groups.
Test group: after sunset, placing the seedlings of the Chinese juniper and the seedling raising container on a shaking seedling bed at 80rpm, shaking at a constant speed, and closing the shaking table after sunrise for the next day;
control group 3: similar to the test group, only the shaker speed was varied, set at 40rpm;
control group 4: similar to the test group, only the shaker speed was varied, set at 120rpm;
the environmental condition settings of the three groups during the trial were consistent with the acclimation period and the trial continued for 2 weeks. After the test is finished, the 3 treated plant heights, the ground diameters and the ratio of the ground diameters to the plant heights are counted, and the test results are shown in Table 7;
TABLE 7 influence of shaking speed on the growth traits of seedlings of Styrax
Height of plant (cm) Ground diameter (cm) Ground diameter/plant height
Test group 29.61±3.27 0.45±0.15 0.015±0.002
Control group 3 35.74±2.54* 0.42±0.02 0.012±0.001*
Control group 4 30.68±4.67 0.38±0.11 0.012±0.001*
Note that: * Indicating that the control group was significantly different from the test group.
Analysis of results: it is observed that the seedlings of the control group 3 have a significant difference from the test group in the ratio of ground diameter to plant height, while the root systems of all plants of the control group 4 are exposed because most of matrix is thrown out, the growth of the seedlings is seriously affected, and the ratio of ground diameter to plant height is also significantly lower than that of the test group.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (7)

1. A method for improving the seedling quality of the tree is characterized in that the tree seedlings are placed on a shaking table for uniform shaking culture, and the tree seedlings with high quality are obtained after the culture is finished.
2. The method for improving the seedling raising quality of the tree seedlings according to claim 1, comprising the following steps:
(1) Matrix pretreatment and seed sowing: sterilizing the matrix for raising seedlings of the tree, filling the matrix into a seedling raising container, watering fully, and sowing into germinated seeds;
(2) Seed pre-culture: placing the sown seedling raising container in a greenhouse, and culturing under full illumination until the leaves of the seedlings grow to 4-5 pairs to obtain pre-cultured seedlings;
(3) Culturing seedlings by shaking: the culture conditions are kept unchanged, the pre-cultured seedlings are placed on the shaking table after sunset, the seedlings are cultured in a shaking mode at a constant speed, and the shaking table is closed after sunset, so that the quality of the seedlings can be improved.
3. The method according to claim 2, wherein the method for preparing the aromatic tree seedling substrate in step (1) comprises the following steps: perlite, vermiculite, peat: the coconut husk is prepared by mixing according to the volume ratio of 1:1:2:2; the sowing depth of the seeds is 0.5-1mm.
4. The method according to claim 2, wherein the greenhouse plenoptic culture conditions of step (2) are as follows: the temperature in the daytime is 25-30 ℃, the air humidity is 50-80%, the temperature in the night is 15-18 ℃ and the air humidity is 50-80%.
5. The method according to claim 2, wherein the greenhouse plenoptic culture conditions of step (2) are as follows: the temperature of the air is 28 ℃ in the daytime, the air humidity is 55%, the temperature of the air is 17 ℃ at night and the air humidity is 75%.
6. The method of claim 2, wherein the shaking culture time of step (3) is: 3-10 months, 5-6d per week, 8-10h per day; the shaking culture speed is as follows: 60-90rpm.
7. The method of claim 2, wherein the shaking culture speed of step (3) is: 80rpm.
CN202310922321.6A 2023-07-26 2023-07-26 Method for improving seedling quality of aromatic tree Pending CN116724871A (en)

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CN202310922321.6A CN116724871A (en) 2023-07-26 2023-07-26 Method for improving seedling quality of aromatic tree

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Application Number Priority Date Filing Date Title
CN202310922321.6A CN116724871A (en) 2023-07-26 2023-07-26 Method for improving seedling quality of aromatic tree

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