CN111919751A - Tissue culture method for murraya paniculata seeds - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
Abstract
The invention discloses a tissue culture method of murraya paniculata seeds, which comprises the following steps of: peeling some murraya paniculata seeds in sequence, spraying 75% alcohol to the peeled murraya paniculata seeds in sequence after peeling, then pouring the alcohol into mercuric chloride solution for 30s of treatment, washing the seeds clean with sterile water after the treatment, inoculating the peeled and sterilized murraya paniculata seeds into an induction culture medium for bud induction culture, and performing proliferation culture: sequentially inoculating the buds germinated in the S1 on a multiplication culture medium A and a multiplication culture medium B, differentiating cluster buds, and culturing strong seedlings: and (3) inoculating the cluster buds differentiated in the step (2) in a strong seedling culture medium for culture. The method for culturing the murraya paniculata seed tissue takes seeds as explants, induces germination and germination, firstly inoculates the seeds to a multiplication culture medium A with high hormone concentration, induces cluster buds, and then inoculates the seeds in a multiplication culture medium B with low hormone concentration, thereby being completely suitable for large-scale industrial production.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to a method for culturing murraya paniculata seed tissue.
Background
The murraya paniculata is a small tree, the murraya paniculata is one of common seasonings in our lives, peculiar smell can be removed, the murraya paniculata can be used as a seasoning and can also be used as an ornamental plant, the murraya paniculata is evergreen in four seasons, flowers can have thick fragrance during blooming, fruits are red and gorgeous, south areas are mostly used as fence materials or are used as ornaments of flower gardens and hotels, and therefore the murraya paniculata can be used as potted plants for indoor appreciation, and meanwhile, the murraya paniculata also has certain medicinal value, so that the murraya paniculata is widely planted, and further, a tissue culture method of murraya paniculata seeds is widely researched;
in recent years, researchers research asexual propagation modes such as cutting seedling raising, grafting seedling raising and the like of murraya paniculata, but the asexual propagation modes are limited by seasons and material resources, the survival rate and the emergence rate of grafted seedlings and cutting seedlings are low, and large-scale breeding cannot be efficiently carried out;
therefore, a method for tissue culture of the seeds of the murraya paniculata is provided so as to solve the problems well.
Disclosure of Invention
The invention aims to provide a tissue culture method for murraya paniculata seeds, which aims to solve the problems that the prior art is limited by seasons and material resources, the survival rate and the emergence rate of grafted seedlings and cutting seedlings are low, large-scale breeding cannot be efficiently carried out, and the prior art cannot be suitable for large-scale forestry production.
In order to achieve the purpose, the invention provides the following technical scheme: a tissue culture method of Murraya paniculata seed comprises the following steps,
s1: and (3) induction culture: peeling off some murraya paniculata seeds in sequence, spraying 75% alcohol to the peeled murraya paniculata seeds in sequence after peeling, then pouring the alcohol into mercuric chloride solution for treatment for 30s, washing the seeds clean by sterile water after the treatment is finished, and then inoculating the peeled and sterilized murraya paniculata seeds into an induction culture medium for bud induction culture;
s2: and (3) proliferation culture: sequentially inoculating the buds germinated in the S1 on a multiplication culture medium A and a multiplication culture medium B, and differentiating cluster buds;
s3: strong seedling culture: inoculating the cluster buds differentiated in the step (2) in a strong seedling culture medium for culture, and giving certain nutrient components to the cluster buds through the strong seedling culture medium to ensure that the cluster buds grow into strong seedlings;
s4: rooting culture: firstly placing a rooted tissue culture seedling in a greenhouse for hardening the seedling for a period of time, then opening a cover to take the seedling, cleaning the strong seedling, then treating the strong seedling with 1000 times of carbendazim or thiophanate methyl for a period of time, then placing the treated strong seedling in a matrix for cultivation, cutting multiple buds of the strong seedling into single plants, inoculating the single plants into a rooting culture medium for cultivation, continuing cultivation after rooting, obtaining a complete plant with roots after inoculating the complete plant with roots onto the rooting culture medium for 25 days, obtaining cinnamomum kanehirae seedling when the rooted seedling grows to 5-8 cm and the number of the roots is more than 3, and then transplanting the cinnamomum kanehirae seedling outdoors for cultivation.
Preferably, the volume fraction of the alcohol in the S1 is 75%, the treatment time is 30S, the mercury bichloride treatment time is 5-6 min, the treatment time of the S1 is 20-30S, and 70-75% of the alcohol has strong permeability.
Preferably, the cotyledon of the murraya paniculata seeds subjected to induction culture in the step S1 is germinated, the leaves are unfolded, and the period of sprouting is 7-10 days after the seeds are inoculated to the induction culture medium, and the seeds can be inoculated to the proliferation culture medium in the step S2 after the sprouts sprout.
Preferably, the enrichment medium A in S2 comprises an MS medium, 6-BA2.0mg/L, IBA0.5mg/L, sucrose 30g/L, carrageenan 6.5g/L and a pH value of 5.8, the enrichment medium B comprises an MS medium, 6-BA1.5mg/L, IBA0.05mg/L, sucrose 30g/L, agar 6.5g/L and a pH value of 5.8, all culture conditions in S1, S2 and S3 are illumination for 10-12 hours every day, and the temperature is 25-30 ℃.
Preferably, the culture time of the proliferation culture medium A in S2 is 40-50 days (generation 2).
Preferably, the culture time of the proliferation medium B in S2 is 180-200 days (9-10 generations).
Preferably, the induction medium in S1 includes: MS culture medium, 6-BA2.0mg/L, IBA0.5mg/L, active carbon 0.5g/L, cane sugar 30g/L, carrageenan 6.5g/L, pH value 5.8.
Preferably, the strong seedling culture medium in S3 comprises: MS culture medium, peptone 1.0mg/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
Preferably, the rooting medium in S4 comprises: 1/2MS culture medium, IBA1.0mg/L, active carbon 1.5g/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
Preferably, the hardening-seedling days in the S4 are 10-15 days, strong seedlings in the S4 are treated by 1000 times of carbendazim or thiophanate methyl for 10-15min, and the matrix in the S4 is peat soil and perlite according to the volume ratio of 2: 1 mixing the components.
Preferably, all the culture conditions in S1, S2 and S3 are light for 10-12 h every day and the temperature is 25-30 ℃.
Compared with the prior art, the invention has the beneficial effects that: the tissue culture method of the murraya paniculata seeds comprises the following steps:
(1) the invention takes seeds as explants to induce germination, firstly inoculates the seeds to a multiplication culture medium A with high hormone concentration to induce cluster buds, then inoculates the seeds in a multiplication culture medium B with low hormone concentration to differentiate a large number of normal cluster buds, the monthly propagation coefficient reaches 3 to 4 times, and the invention is completely suitable for large-scale industrial production so as to be convenient for well performing seed tissue culture;
(2) the method effectively solves the problem of low cuttage efficiency and low propagation efficiency of the seedlings of the murraya paniculata;
(3) the invention can rapidly produce seedlings in batches and provides sufficient materials for the production of traditional Chinese medicinal materials and the processing of medicaments.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: a tissue culture method of Murraya paniculata seed comprises the following steps,
s1: and (3) induction culture: peeling off some murraya paniculata seeds in sequence, spraying 75% alcohol to the peeled murraya paniculata seeds in sequence after peeling, then pouring the alcohol into mercuric chloride solution for treatment for 30s, washing the seeds clean by sterile water after the treatment is finished, and then inoculating the peeled and sterilized murraya paniculata seeds into an induction culture medium for bud induction culture;
s2: and (3) proliferation culture: sequentially inoculating the buds germinated in the S1 on a multiplication culture medium A and a multiplication culture medium B, and differentiating cluster buds;
s3: strong seedling culture: inoculating the cluster buds differentiated in the step (2) in a strong seedling culture medium for culture, and giving certain nutrient components to the cluster buds through the strong seedling culture medium to ensure that the cluster buds grow into strong seedlings;
s4: rooting culture: firstly placing a rooted tissue culture seedling in a greenhouse for hardening the seedling for a period of time, then opening a cover to take the seedling, cleaning the strong seedling, then treating the strong seedling with 1000 times of carbendazim or thiophanate methyl for a period of time, then placing the treated strong seedling in a matrix for cultivation, cutting multiple buds of the strong seedling into single plants, inoculating the single plants into a rooting culture medium for cultivation, continuing cultivation after rooting, obtaining a complete plant with roots after inoculating the complete plant with roots onto the rooting culture medium for 25 days, obtaining cinnamomum kanehirae seedling when the rooted seedling grows to 5-8 cm and the number of the roots is more than 3, and then transplanting the cinnamomum kanehirae seedling outdoors for cultivation.
Experimental research shows that after the axillary buds are cultured for a period of time by the enrichment medium A, the axillary buds grow quickly, a large number of differentiated buds can be cultured in the enrichment medium B for several generations, the hormone concentration is reduced, normal growth of buds is facilitated, and the monthly propagation coefficient reaches 3-4 times.
The invention establishes a set of efficient tissue culture method for the seeds of the murraya paniculata for the first time, and lays a foundation for the production of the murraya paniculata seedlings with high medicinal value.
The volume fraction of the alcohol in the S1 is 75%, the treatment time is 30S, the mercuric chloride treatment time is 5-6 min, the treatment time of the S1 is 20-30S, and 70-75% of the alcohol has strong permeability.
And (3) performing induction culture in S1 to sprout cotyledons of the murraya paniculata seeds, then expanding leaves, and inoculating the cotyledons to an induction culture medium for 7-10 days to sprout new sprouts, and then inoculating the germinated new sprouts to a proliferation culture medium in S2.
The enrichment culture medium A in S2 comprises an MS culture medium, 6-BA2.0mg/L, IBA0.5mg/L, 30g/L of sucrose, 6.5g/L of carrageenan and 5.8 of pH value, the enrichment culture medium B comprises an MS culture medium, 6-BA1.5mg/L, 0.05mg/L of IBA0, 30g/L of sucrose, 6.5g/L of agar and 5.8 of pH value, all culture conditions in S1, S2 and S3 are all illumination for 10-12 hours every day, and the temperature is 25-30 ℃.
The growth medium A in S2 was cultured for 40-50 days (passage 2).
The culture time of the proliferation culture medium B in S2 is 180-200 days (9-10 generations).
The induction medium in S1 includes: MS culture medium, 6-BA2.0mg/L, IBA0.5mg/L, active carbon 0.5g/L, cane sugar 30g/L, carrageenan 6.5g/L, pH value 5.8.
The strong seedling culture medium in the S3 comprises: MS culture medium, peptone 1.0mg/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
The rooting medium in S4 comprises: 1/2MS culture medium, IBA1.0mg/L, active carbon 1.5g/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
The hardening-seedling days in the S4 are 10-15 days, strong seedlings in the S4 are treated by 1000 times of carbendazim or thiophanate methyl for 10-15min, and the matrix in the S4 is peat soil and perlite according to the volume ratio of 2: 1 mixing the components.
All the culture conditions in S1, S2 and S3 are illumination for 10-12 h every day at 25-30 ℃.
Example 1
Influence of different induction media on induction of murraya paniculata seeds:
the induction culture medium consists of a basic culture medium and a hormone combination, wherein the basic culture medium comprises the following components: MS culture medium, 30g/L of cane sugar, 6.5g/L of carrageenan and 5.8 of pH value.
30 seeds were inoculated for each treatment, the experiment was repeated 3 times, and the germination time, average induction rate and average induced bud number were counted 25 days after explant induction culture.
TABLE 1 influence of different hormone ratios on the induction of seed germination
As can be seen from Table 1, different concentrations of hormones have an effect on the average germination time and the average induction rate. When the 6-BA is 2.0mg/L and the IBA is 0.5mg/L, the inductivity is 100 percent in a short time (8-10 days).
Example 2
Influence of different proliferation media on proliferation culture of murraya paniculata:
the buds induced in the example 1 are inoculated into a proliferation culture medium (the specific formula of the proliferation culture medium is shown in table 3) for proliferation culture, the culture illumination intensity is 2000-3000 lux, and the illumination time is 12 hours/day.
The proliferation culture medium consists of a basic culture medium and a hormone combination, wherein the basic culture medium comprises the following components: MS culture medium, 30g/L of cane sugar, 6.5g/L of carrageenan and 5.8 of pH value.
30 shoots were inoculated per treatment and the experiment was repeated 3 times, and for each shoot proliferation culture generation (i.e., every 25 days), the corresponding proliferation factor and shoot growth were counted.
TABLE 2 Effect of different hormone ratios on Cluster bud proliferation
As can be seen from Table 2, the medium hormone concentrations varied with time. The concentration of 6-BA is lower than 1.5mg/ml, the growth condition of adventitious buds is better, the multiplication coefficient is lower, if only one multiplication culture medium is adopted in the multiplication culture process, the first two generations of seedlings are strong and strong, a large number of slave buds are generated, but the later generations of buds are micro-vitrified, and a large number of seedlings die, or the first two generations of seedlings are general in growth vigor, slow in cluster bud induction, weak in total bud body and low in multiplication coefficient, and the two culture media can not achieve the optimal effect of the multiplication culture of the cinnamomum kanehirae, so that the optimal scheme for the multiplication of the murraya paniculata in the test comprises the steps of firstly inoculating the buds into a basic culture medium + BA2.5+ IBA0.5 culture medium to culture for 40-0 day, and after the buds are induced to grow, inoculating into the basic culture medium 6-BA1.5+ IBA0.5 to culture, and thus not only improving the multiplication rate, but also enabling the normal growth of
Example 3
Influence of different rooting media on the rooting of the murraya paniculata seedlings:
after culturing the cluster buds in a strong seedling culture medium for 20-25 days, cutting the cluster buds into single plants, inoculating the single plants to a rooting culture medium (the specific formula of the rooting culture medium is shown in table 3) for rooting culture, wherein the culture illumination intensity is 3000-3500 lux, the illumination time is 12 hours/day, each culture medium is inoculated with 10 seedlings, the test is repeated for 3 times, and after rooting culture for 25 days, the rooting rate, the average number of roots per plant and the growth vigor of the plants are counted.
The rooting culture medium consists of a basic culture medium and a hormone composition, wherein the basic culture medium comprises the following components: 1/2MS culture medium, active carbon 1.5g/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
TABLE 3 influence of different hormone ratios on rooting of Murraya paniculata tissue culture seedlings
As can be seen from Table 3, when the concentration is higher than 1.5mg/L, the Murraya paniculata is not favorable for rooting, the comprehensive comparison of all indexes is used for analysis, IBA1.5mg/L is selected as an optimal rooting culture medium, the rooting rate under the combination is 98.6%, and the average number of roots per plant is 3.32.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A method for culturing Murraya paniculata seed tissues is characterized by comprising the following steps:
s1: and (3) induction culture: peeling off some murraya paniculata seeds in sequence, spraying 75% alcohol to the peeled murraya paniculata seeds in sequence after peeling, then pouring the alcohol into mercuric chloride solution for treatment for 30s, washing the seeds clean by sterile water after the treatment is finished, and then inoculating the peeled and sterilized murraya paniculata seeds into an induction culture medium for bud induction culture;
s2: and (3) proliferation culture: sequentially inoculating the buds germinated in the S1 on a multiplication culture medium A and a multiplication culture medium B, and differentiating cluster buds;
s3: strong seedling culture: inoculating the cluster buds differentiated in the step (2) in a strong seedling culture medium for culture, and giving certain nutrient components to the cluster buds through the strong seedling culture medium to ensure that the cluster buds grow into strong seedlings;
s4: rooting culture: firstly placing a rooted tissue culture seedling in a greenhouse for hardening the seedling for a period of time, then opening a cover to take the seedling, cleaning the strong seedling, then treating the strong seedling with 1000 times of carbendazim or thiophanate methyl for a period of time, then placing the treated strong seedling in a matrix for cultivation, cutting multiple buds of the strong seedling into single plants, inoculating the single plants into a rooting culture medium for cultivation, continuing cultivation after rooting, obtaining a complete plant with roots after inoculating the complete plant with roots onto the rooting culture medium for 25 days, obtaining cinnamomum kanehirae seedling when the rooted seedling grows to 5-8 cm and the number of the roots is more than 3, and then transplanting the cinnamomum kanehirae seedling outdoors for cultivation.
2. The method for tissue culture of seeds of groundsel of claim 1, wherein: the volume fraction of the alcohol in the S1 is 75%, the treatment time is 30S, the mercury bichloride treatment time is 5-6 min, the treatment time of the S1 is 20-30S, and 70-75% of the alcohol has strong permeability.
3. The method for tissue culture of seeds of groundsel of claim 1, wherein: and (3) the cotyledon of the murraya paniculata seeds is subjected to induction culture in the S1, the leaves are unfolded, and the time for sprouting is 7-10 days after the seeds are inoculated to an induction culture medium, and the seeds can be inoculated to a proliferation culture medium in the S2 after sprouting.
4. The method for tissue culture of seeds of groundsel of claim 1, wherein: the enrichment medium A in the S2 comprises an MS medium, 6-BA2.0mg/L, IBA0.5mg/L, 30g/L of cane sugar, 6.5g/L of carrageenan and 5.8 of pH value, the enrichment medium B comprises an MS medium, 6-BA1.5mg/L, IBA0.05mg/L, 30g/L of cane sugar, 6.5g/L of agar and 5.8 of pH value, all culture conditions in the S1, the S2 and the S3 are all illumination for 10-12 hours every day, and the temperature is 25-30 ℃.
5. The method for tissue culture of seeds of groundsel of claim 1, wherein: the culture time of the proliferation culture medium A in the S2 is 40-50 days (2 generations).
6. The method for tissue culture of seeds of groundsel of claim 1, wherein: the culture time of the proliferation culture medium B in the S2 is 180-200 days (9-10 generations).
7. The method for tissue culture of seeds of groundsel of claim 1, wherein: the induction medium in S1 includes: MS culture medium, 6-BA2.0mg/L, IBA0.5mg/L, active carbon 0.5g/L, cane sugar 30g/L, carrageenan 6.5g/L, pH value 5.8.
8. The method for tissue culture of seeds of groundsel of claim 1, wherein: the strong seedling culture medium in the S3 comprises: MS culture medium, peptone 1.0mg/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
9. The method for tissue culture of seeds of groundsel of claim 1, wherein: the rooting medium in S4 comprises: 1/2MS culture medium, IBA1.0mg/L, active carbon 1.5g/L, sucrose 30g/L, carrageenan 6.5g/L, pH 5.8.
10. The method for tissue culture of seeds of groundsel of claim 1, wherein: the hardening-seedling days in the S4 are 10-15 days, strong seedlings in the S4 are treated by 1000 times of carbendazim or thiophanate methyl for 10-15min, and the matrix in the S4 is peat soil and perlite according to the volume ratio of 2: 1 mixing the components.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102227976A (en) * | 2011-04-11 | 2011-11-02 | 广西壮族自治区林业科学研究院 | Litsea cubeba pers asexual quick propagation and seedling breeding method |
| US20150252380A1 (en) * | 2003-05-14 | 2015-09-10 | Integrated Plant Genetics, Inc. | Use of bacteriophage outer membrane breaching proteins expressed in plants for the control of gram-negative bacteria |
| CN106358819A (en) * | 2016-08-25 | 2017-02-01 | 合肥市田然农业科技园有限公司 | Chrysanthemum cuttage substrate |
| CN106717974A (en) * | 2017-01-05 | 2017-05-31 | 华润三九医药股份有限公司 | A kind of cuttage and seedling culture method of murraya paniculataJack |
| CN106718890A (en) * | 2016-12-02 | 2017-05-31 | 河池学院 | A kind of method for tissue culture suitable for Chinese herbal medicine murraya paniculataJack seedling |
| CN108770688A (en) * | 2018-04-26 | 2018-11-09 | 华润三九医药股份有限公司 | A kind of rapid propagation method of murraya paniculataJack |
| US20190343122A1 (en) * | 2016-05-27 | 2019-11-14 | Provivi, Inc. | Improved insect control strategies utilizing pheromones and rnai |
| CN110720393A (en) * | 2019-11-01 | 2020-01-24 | 中国计量大学 | Method for tissue culture and rapid propagation of ficus microcarpa |
-
2020
- 2020-08-31 CN CN202010896435.4A patent/CN111919751B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150252380A1 (en) * | 2003-05-14 | 2015-09-10 | Integrated Plant Genetics, Inc. | Use of bacteriophage outer membrane breaching proteins expressed in plants for the control of gram-negative bacteria |
| CN102227976A (en) * | 2011-04-11 | 2011-11-02 | 广西壮族自治区林业科学研究院 | Litsea cubeba pers asexual quick propagation and seedling breeding method |
| US20190343122A1 (en) * | 2016-05-27 | 2019-11-14 | Provivi, Inc. | Improved insect control strategies utilizing pheromones and rnai |
| CN106358819A (en) * | 2016-08-25 | 2017-02-01 | 合肥市田然农业科技园有限公司 | Chrysanthemum cuttage substrate |
| CN106718890A (en) * | 2016-12-02 | 2017-05-31 | 河池学院 | A kind of method for tissue culture suitable for Chinese herbal medicine murraya paniculataJack seedling |
| CN106717974A (en) * | 2017-01-05 | 2017-05-31 | 华润三九医药股份有限公司 | A kind of cuttage and seedling culture method of murraya paniculataJack |
| CN108770688A (en) * | 2018-04-26 | 2018-11-09 | 华润三九医药股份有限公司 | A kind of rapid propagation method of murraya paniculataJack |
| CN110720393A (en) * | 2019-11-01 | 2020-01-24 | 中国计量大学 | Method for tissue culture and rapid propagation of ficus microcarpa |
Non-Patent Citations (2)
| Title |
|---|
| 蒋建林等: ""四数九里香种子自然萌发和离体萌发条件筛选"", 《经济林研究》 * |
| 蒋烨: ""千里香的组织培养研究"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
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