CN116698710A - Flow cytometer - Google Patents
Flow cytometer Download PDFInfo
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- CN116698710A CN116698710A CN202310868071.2A CN202310868071A CN116698710A CN 116698710 A CN116698710 A CN 116698710A CN 202310868071 A CN202310868071 A CN 202310868071A CN 116698710 A CN116698710 A CN 116698710A
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- 238000001514 detection method Methods 0.000 claims abstract description 108
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 238000005070 sampling Methods 0.000 claims description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 57
- 210000001503 joint Anatomy 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 29
- 238000003825 pressing Methods 0.000 claims description 19
- 238000004891 communication Methods 0.000 claims description 17
- 238000004140 cleaning Methods 0.000 claims description 15
- 230000005611 electricity Effects 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 abstract description 2
- 239000007921 spray Substances 0.000 description 14
- 239000012530 fluid Substances 0.000 description 12
- 238000005406 washing Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The application relates to the field of cell detection technology, and discloses a flow cytometer, which comprises a detection box, an air supply box and a sample injection mechanism, wherein the sample injection mechanism comprises a pushing component and a placing box for containing a sample tube, the placing box is slidingly connected to the detection box, the pushing component is connected with the placing box, and the pushing component is used for driving the placing box to slide into the detection box; the placing box is communicated with the air supply box, and the air supply box is used for supplying constant temperature and humidity air into the placing box. The application can detect the sample cells in the sample tube under the condition of not influencing the detection environment in the detection box, ensures the accuracy of the detection result of the cell sample, does not need to regulate and control the environment of a laboratory, reduces the expenditure of detection cost, can directly use the flow cytometer in other places, and improves the applicability of the flow cytometer.
Description
Technical Field
The application relates to the field of cell detection technology, in particular to a flow cytometer.
Background
Flow cytometry is a device that automatically analyzes and sorts cells. The flow cytometer mainly comprises a liquid flow system, a laser source, an optical system, a detection mechanism, a computer and an analysis system; and extracting and mixing sheath fluid and the cell sample by using a liquid flow system, detecting the cell sample by using a detection mechanism, transmitting detected information to a computer and an analysis system, and obtaining a detection result of the cell sample by using the computer and the analysis system.
When the flow cytometer is used for detecting cells, the environment in the laboratory is required to be adjusted first, so that the environment temperature and the relative humidity in the laboratory are kept within a specified range, the flow cytometer is ensured to operate under specific environment conditions, and the accuracy of test results is ensured.
For the related art, the inventor finds that when the flow cytometer is used to detect the cells, the detection needs to be performed in a laboratory under the regulation of a specific environment, so that the use range of the flow cytometer is smaller, the defect of smaller applicability of the flow cytometer exists, and the regulation of the environment of the whole laboratory consumes larger power resources, so that the detection cost is increased.
Disclosure of Invention
In order to alleviate the problems of low applicability and easy increase of detection cost of the flow cytometer, the application provides a flow cytometer.
The application provides a flow cytometer, which adopts the following technical scheme:
the flow cytometer comprises a detection box, an air supply box and a sample injection mechanism, wherein the sample injection mechanism comprises a pushing component and a placement box for containing a sample tube, the placement box is slidably connected to the detection box, the pushing component is connected with the placement box, and the pushing component is used for driving the placement box to slide into the detection box; the placing box is communicated with the air supply box, and the air supply box is used for supplying constant temperature and humidity air into the placing box.
Through adopting above-mentioned technical scheme, with detection case and air feed case intercommunication, utilize the air feed case to the interior constant temperature and humidity air of feeding of detection case, guarantee that the air environment in the detection case is in steady state, then place one of them and place the incasement with the sample pipe that holds the cell sample, then utilize the air feed case to place the incasement and supply constant temperature and humidity air, restart the promotion subassembly, utilize the promotion subassembly drive to place the case of placing the sample pipe and remove to detection incasement portion, then can detect the sample cell in the sample pipe under the circumstances that does not influence detection case internal detection environment, guarantee the accuracy of cell sample testing result, need not to regulate and control the environment of laboratory moreover, reduce the expenditure of testing cost, can use the flow cytometer directly in other places simultaneously, improve the suitability of flow cytometer.
Preferably, the sample feeding mechanism further comprises a support plate, the support plate is arranged on the detection box in a penetrating mode, the support plate is in the detection box in a sliding mode, the pushing assembly is connected with the support plate to drive the support plate to slide in a reciprocating mode, two placing boxes are arranged, the two placing boxes are fixedly connected to the support plate, the two placing boxes alternately enter the detection box under the driving of the support plate, and the two placing boxes are communicated with the air supply box.
Through adopting above-mentioned technical scheme, set up two and place the case on the supporting plate, when detecting the cell sample, place the cell sample at first in one of them and place the incasement, then utilize the air feed case to adjust the back to the air environment of placing the incasement, promote the subassembly start and drive and place the detection incasement portion that the case got into and detect the in-process to the cell sample in the sample pipe, another place the outside that the case just is located the detection case, at this moment, the staff can place new cell sample to another place the incasement, when detecting the sample cell of placing the incasement to one of them, can take out the sample pipe of another place the incasement simultaneously, and place new sample pipe, improve the detection efficiency of cell sample.
Preferably, the detection box is internally provided with a liquid flow mechanism, the liquid flow mechanism comprises a spray head, a sheath liquid supply assembly and a sample extraction assembly, the spray head is fixedly connected in the detection box, the sheath liquid supply assembly is connected on the detection box, the sheath liquid supply assembly is used for supplying sheath liquid to the spray head, the sample extraction assembly comprises a sampling needle, a sampling tube and an electric pushing cylinder, the electric pushing cylinder is fixedly connected in the detection box, the sampling needle is fixedly connected on a piston rod of the electric pushing cylinder, the electric pushing cylinder drives the sampling needle to lift so as to enable the sampling needle to be inserted into the sampling tube, the sampling tube is communicated with the sampling needle, and one end, far away from the sampling needle, of the sampling tube is communicated with the spray head.
Through adopting above-mentioned technical scheme, when detecting the cell sample, utilize sheath liquid supply subassembly to supply sheath liquid in to the shower nozzle, the piston rod of electricity push away the jar simultaneously starts drive sampling needle and inserts in the sampling tube, makes the sample draw to the cell sample, can supply the cell sample to the shower nozzle in with sheath liquid mix, then spout from the shower nozzle, can detect the cell sample.
Preferably, the air feed box is the constant temperature incubator, be provided with air feed subassembly and two sets of communication subassembly on the air feed box, two sets of communication subassembly and two place the case one-to-one setting, every group communication subassembly all includes communicating pipe and automatically controlled valve, the one end of communicating pipe with inside the air feed box, the other end of communicating pipe rather than corresponding place the case intercommunication, the setting of automatically controlled valve is in on the communicating pipe, automatically controlled valve is used for right opening and close of communicating pipe is controlled, the air feed subassembly be used for with air feed in the air feed box in the communicating pipe.
Through adopting above-mentioned technical scheme, regard constant temperature incubator as the air feed case, utilize the self-regulation ability of constant temperature incubator to air to guarantee the stability of air supply incasement air environment, after placing the case with the cell sample in, start the air feed subassembly, open automatically controlled valve simultaneously, can utilize the air feed subassembly to supply into the air of air feed incasement through corresponding communicating pipe and place the incasement, realize placing the regulation of incasement air environment, after placing the incasement and removing to detecting the incasement portion, place case and detecting incasement portion intercommunication, continue to supply air to placing the incasement and can realize the regulation to detecting incasement air environment.
Preferably, each of the top parts of the placing boxes is provided with a sampling port, the placing boxes are provided with plugging pieces, each plugging piece comprises a first cylinder and a plugging plate, the first cylinders are fixedly connected to the placing boxes, the plugging plates are in sealing sliding connection in the placing boxes, piston rods of the first cylinders are fixedly connected with the plugging plates, and the first cylinders drive the plugging plates to slide so as to control the opening and closing of the sampling ports.
Through adopting above-mentioned technical scheme, after placing the case mouth with the air feed in the air feed case, start with first cylinder, utilize first cylinder drive shutoff board to place the sampling port shutoff that the case top was offered, can avoid placing the constant temperature and humidity air in the case and take place the exchange with outside air, then the drive is placed the case and is got into after detecting the incasement portion, the piston rod shrink of first cylinder can drive and make first cylinder drive shutoff board rebound to make the sampling port open, thereby can sample the cell sample of placing the incasement and detect.
Preferably, the detection box is provided with a cleaning mechanism, the cleaning mechanism comprises a water storage bottle, a water outlet pipe, a butt joint pipe, a water pressing component and a power component, the water storage bottle is fixedly connected to the detection box, the butt joint pipe is slidably connected to the detection box, the butt joint pipe is communicated with the water storage bottle through the water outlet pipe, the water pressing component is connected to the water storage bottle, the water pressing component is used for pressing cleaning water in the water storage bottle into the butt joint pipe, the power component is connected to the detection box, and the power component is connected with the butt joint pipe to drive the butt joint pipe to slide.
Through adopting above-mentioned technical scheme, when accomplishing one kind of cell sample detection, need detect another kind of cell sample, start power component, utilize power component drive butt joint pipe to remove, make the butt joint pipe remove under the sample needle, then start the electric jar that pushes away, make the electric jar drive sample needle insert in the butt joint pipe, then make the sealed butt joint of sample needle and butt joint pipe, then the pressurized-water subassembly is pressed the washing water in the water storage bottle into the butt joint pipe through the outlet pipe, then in the rethread sample needle gets into the wash pipe, realize the washing to sample needle and sampling tube, improve the abluent convenience to sample needle and sampling tube, then detect another kind of cell sample again, guarantee the accuracy to follow-up cell sample testing result.
Preferably, the water pressing assembly comprises an air supply pipe, a control valve and a first vent pipe, wherein the air supply pipe is communicated with the inside of the air supply box, one end, far away from the air supply box, of the air supply pipe is communicated with the control valve, the first vent pipe is communicated with an air outlet of the control valve, and one end, far away from the control valve, of the first vent pipe is communicated with the inside of the water storage bottle from the top of the water storage bottle.
Through adopting above-mentioned technical scheme, when wasing the sampling tube, close two automatically controlled valves, open the control valve, then will supply the air in the gas tank and get into first breather pipe through the air supply pipe, then get into the water storage bottle in to can exert pressure the wash water in the water storage bottle, in will wash the water pressure sampling tube, solid washing to the sampling tube.
Preferably, the control valve is a three-way valve, the first vent pipe is communicated with one air outlet of the control valve, the other air outlet of the control valve is communicated with a second vent pipe, and one end, away from the control valve, of the second vent pipe is communicated with the butt joint pipe.
By adopting the technical scheme, the air supply pipe is communicated with the first vent pipe through the control valve by utilizing the control valve, so that the air in the air supply box can be pressed into the water storage bottle, and the cleaning of the sampling pipe is realized; after the sampling tube is cleaned, the air supply tube is communicated with the second vent tube through the control valve, and gas in the air supply box can be directly supplied into the second vent tube, so that the gas in the air supply tube can quickly enter the butt joint tube, then quickly passes through the sampling tube, and residual moisture in the sampling tube and the spray head is blown off and dried by utilizing air flow, so that the cleaning and drying speed of the sampling tube is improved.
Preferably, the power assembly comprises a second motor and a supporting plate, the second motor is fixedly connected in the detection box, the supporting plate is fixedly connected to a main shaft of the second motor, and the butt joint pipe is fixedly connected to the supporting plate.
Through adopting above-mentioned technical scheme, with butt joint pipe fixed connection in the backup pad, utilize second motor drive backup pad rotation can adjust the position of butt joint pipe, improve the convenience of butt joint pipe position adjustment.
Preferably, each of the placement boxes is provided with a first one-way valve, and the first one-way valve enables air in the placement box to flow in a one-way manner outwards from the interior of the placement box.
Through adopting above-mentioned technical scheme, utilize the setting of first check valve, put into the box of placing with the cell sample after, close the sampling port, then will supply the air feed in the air feed case again and place the case in, place the incasement original air and discharge and place outside the case through first check valve, avoid placing the incasement air and adjust the back, outside air gets into again in sampling port department and places incasement portion's possibility.
In summary, the application at least comprises the following beneficial technical effects:
1. through the communication of the detection box and the air supply box, the constant temperature and constant humidity air is supplied into the detection box by the air supply box, the sample cells in the sample tube are detected under the condition that the detection environment in the detection box is not influenced, the accuracy of the detection result of the cell sample is ensured, the environment of a laboratory is not required to be regulated and controlled, the expenditure of the detection cost is reduced, the flow cytometer can be directly used in other places, and the applicability of the flow cytometer is improved;
2. by arranging two placing boxes on the supporting plate, when the sample cells in one placing box are detected, the sample tubes in the other placing box can be simultaneously taken out, new sample tubes are placed, and the detection efficiency of the cell samples is improved;
3. through setting up in the detection box and to take over, detect one of them cell sample and accomplish, need detect when another kind of cell sample, remove the butt joint pipe to the below of sampling needle, then make the sealed butt joint of sampling needle and butt joint pipe, the pressurized-water subassembly is pressed the washing water in the water storage bottle into the butt joint pipe through the outlet pipe, then in the rethread sampling needle gets into the washing pipe, realize the washing to sampling needle and sampling tube, improve the abluent convenience to sampling needle and sampling tube, then detect another kind of cell sample again, guarantee the accuracy to follow-up cell sample testing result.
Drawings
FIG. 1 is a schematic overall structure of an embodiment of the present application;
FIG. 2 is a schematic cross-sectional view of a detection box according to an embodiment of the present application;
FIG. 3 is a schematic view of a fluid flow mechanism in accordance with an embodiment of the present application;
FIG. 4 is a schematic view of a feeding mechanism according to an embodiment of the present application;
FIG. 5 is a schematic view of the structure of the placement box in the embodiment of the present application;
FIG. 6 is a schematic view of a gas supply assembly in accordance with an embodiment of the present application;
fig. 7 is a schematic view of a cleaning mechanism according to an embodiment of the present application.
Reference numerals: 100. a detection box; 200. a gas supply tank; 210. a gas supply assembly; 211. a second cylinder; 212. a lower pressing plate; 213. a second one-way valve; 300. a liquid flow mechanism; 310. a spray head; 320. a sheath fluid supply assembly; 321. a sheath liquid barrel; 322. a sheath fluid tube; 323. a first transfer pump; 330. a sample extraction assembly; 331. an electric pushing cylinder; 332. a sampling needle; 333. a sampling tube; 334. a second transfer pump; 400. a sample injection mechanism; 410. a bearing plate; 420. placing a box; 421. a sampling port; 422. a first one-way valve; 430. a pushing assembly; 431. a first motor; 432. a gear; 433. a rack; 440. a communication assembly; 441. a communicating pipe; 442. an electric control valve; 450. a blocking member; 451. a first cylinder; 452. a plugging plate; 460. a third motor; 461. a tray; 500. a cleaning mechanism; 510. a water storage bottle; 520. a water outlet pipe; 530. a butt joint pipe; 540. a power assembly; 541. a second motor; 542. a support plate; 550. a water pressing assembly; 551. an air supply pipe; 552. a control valve; 553. a first vent pipe; 554. and a second vent pipe.
Detailed Description
The application is described in further detail below with reference to fig. 1-7.
The embodiment of the application discloses a flow cytometer.
Referring to fig. 1 and 2, a flow cytometer includes a detection box 100 and an air supply box 200, and a sample tube containing a cell sample is placed inside the detection box 100. A flow mechanism 300 is mounted in the detection chamber 100, and the flow mechanism 300 is used for extracting a cell sample in a sample tube. A detection mechanism for detecting a cell sample is mounted in the detection box 100. The air supply box 200 is communicated with the inside of the detection box 100, and the air supply box 200 can supply constant temperature and humidity air into the detection box 100. When the cell sample is detected, the constant temperature and constant humidity air is supplied into the detection box 100 by the air supply box 200 by utilizing the closed arrangement of the detection box 100, so that the detection box 100 is internally kept with a proper detection environment, the accuracy of the detection result of the cell sample is further ensured, the environment of a laboratory is not required to be regulated and controlled, the expenditure of the experiment cost is reduced, the flow cytometer can be directly used in other places, and the applicability of the flow cytometer is improved.
Referring to fig. 2 and 3, the fluid flow mechanism 300 includes a spray head 310, and the spray head 310 is fixedly connected to the inside of the detection chamber 100. The sheath liquid supply assembly 320 is arranged in the detection box 100, the sheath liquid supply assembly 320 comprises a sheath liquid barrel 321, the sheath liquid barrel 321 is fixed on one side of the detection box 100, sheath liquid is contained in the sheath liquid barrel 321, a sheath liquid pipe 322 is communicated with the sheath liquid barrel 321, and one end, far away from the sheath liquid barrel 321, of the sheath liquid pipe 322 is communicated with the inside of the spray head 310; the sheath fluid pipe 322 is provided with a first delivery pump 323, and the sheath fluid in the sheath fluid barrel 321 enters the spray head 310 through the sheath fluid pipe 322 under the driving of the first delivery pump 323.
Install sample extraction subassembly 330 in the detection case 100, sample extraction subassembly 330 includes electricity pushes away jar 331, electricity pushes away jar 331 fixed connection is inside detection case 100, electricity pushes away jar 331 vertical setting, the piston rod fixedly connected with of electricity pushes away jar 331 slides the piece, slide the piece and follow vertical direction sliding connection inside detection case 100, fixed connection sample needle 332 on the piece slides, the top intercommunication of sample needle 332 has sampling tube 333, sampling tube 333 is kept away from the one end and the inside intercommunication of shower nozzle 310 of sample needle 332, install the second delivery pump 334 on the sampling tube 333. When sampling a cell sample, the electric push cylinder 331 is started first, the electric push cylinder 331 drives the sampling needle 332 to move downwards, the sampling needle 332 is inserted into the sample tube, then the second transfer pump 334 is started, the sample of the sample tube can be pumped into the spray head 310 through the sampling tube 333, meanwhile, the first transfer pump 323 pumps sheath fluid, the sheath fluid is supplied into the spray head 310, the sample and the sheath fluid are mixed and then flow out from the spray head 310, and then the detection mechanism is utilized for detection.
Referring to fig. 1, 2 and 4, in order to reduce the influence on the air environment in the detection box 100 when the sample tube is replaced, the sample injection mechanism 400 is mounted on the detection box 100, the sample injection mechanism 400 includes a support plate 410 penetrating through the detection box 100, the support plate 410 is slidably connected to the detection box 100, two placing boxes 420 are fixedly connected to the support plate 410, the two placing boxes 420 are arranged side by side along the sliding direction of the support plate 410, and when one of the placing boxes 420 slides into the detection box 100, the other placing box 420 is positioned outside the detection box 100. The air supply box 200 is provided with two groups of communication components 440, the two groups of communication components 440 are arranged in one-to-one correspondence with the two placing boxes 420, and the placing boxes 420 are communicated with the air supply box 200 through the corresponding communication components 440. The detection box 100 is mounted with a pushing assembly 430, and the pushing assembly 430 is used for driving the support plate 410 to reciprocate.
Referring to fig. 4, the pushing assembly 430 includes a first motor 431 fixedly connected in the detection box 100, a first gear 432 is coaxially and fixedly connected to a spindle of the first motor 431, a first rack 433 is fixedly connected to a bottom of the support plate 410, a length direction of the first rack 433 is parallel to a length direction of the support plate 410, and the first gear 432 is engaged with the first rack 433.
Referring to fig. 1, 2 and 5, each of the communication assemblies 440 includes a communication pipe 441, one end of the communication pipe 441 is communicated with the inside of the placement tank 420, the other end of the communication pipe 441 is communicated with the corresponding gas supply tank 200, and an electronic control valve 442 is mounted on the communication pipe 441, and the electronic control valve 442 is used for controlling the opening and closing of the communication pipe 441.
Referring to fig. 1, 2 and 5, the sampling port 421 is formed at the top of each placement box 420, the placement boxes 420 are provided with the plugging members 450, the plugging members 450 comprise first air cylinders 451 fixedly connected to the placement boxes 420, piston rods of the first air cylinders 451 are fixedly connected with plugging plates 452, the plugging plates 452 are in sealing sliding connection with the top wall of the placement boxes 420, and the plugging plates 452 are used for controlling the opening or closing of the sampling ports 421. The first check valve 422 is fixedly connected to the body of the placement box 420, the first check valve 422 is communicated with the interior of the placement box 420, and air can only flow outwards from the interior of the placement box 420 through the first check valve 422. When a cell sample is detected, firstly, a sample tube is placed in the placement box 420, then the air supply box 200 supplies constant temperature and humidity air into the placement box 420, after the air environment in the placement box 420 reaches detection conditions, the first motor 431 is started, the main shaft of the first motor 431 rotates to drive the gear 432 which is fixedly connected with the main shaft coaxially to rotate, so that the support plate 410 can be driven to slide under the cooperation of the rack 433, the support plate 410 drives the placement box 420 to move into the detection box 100, then the piston rod of the first cylinder 451 contracts to enable the sampling port 421 to be opened, then the sample cells in the sample tube can be detected under the condition that the detection environment in the detection box 100 is not influenced, at the moment, the other placement box 420 moves out of the detection box 100 under the driving of the support plate 410, and when the sample cells in one placement box 420 are detected, the sample tube in the other placement box 420 can be simultaneously taken out, the direction is novel, and the detection efficiency of the cell sample is improved.
Referring to fig. 1 and 6, the air supply tank 200 is a constant temperature incubator, and a inspector can adjust the environment in the air supply tank 200 according to the need. The air supply assembly 210 is installed on the air supply box 200, the air supply assembly 210 comprises a second air cylinder 211 fixedly connected to the top of the air supply box 200, the second air cylinder 211 is vertically arranged, a piston rod of the second air cylinder 211 penetrates through the top wall of the placement box 420 and then is fixedly connected with a lower pressing plate 212, the lower pressing plate 212 is in sealing sliding connection with the inside of the air supply box 200, a second one-way valve 213 is fixedly connected to the side wall of the air supply box 200, the second one-way valve 213 is communicated with the inside of the air supply box 200, and air can flow from the external environment to the inside of the air supply box 200 only through the second one-way valve 213. The lower pressure plate 212 is driven to move upwards by the second air cylinder 211, so that air in the external environment can enter the air supply box 200, then the air supply box 200 regulates and controls the air entering the air supply box according to set parameters, when the air in the air supply box 200 needs to be introduced into the placement box 420 or the detection box 100, the second air cylinder 211 is started, the corresponding electric control valve 442 is opened, the lower pressure plate 212 is driven to move downwards by the second air cylinder 211, and the air in the air supply box 200 can enter the air supply box 200 through the communicating pipe 441, so that the convenience of regulating and controlling the environment in the detection box is improved.
Referring to fig. 5, in order to place a plurality of sample tubes in the placement boxes 420 at a time, a third motor 460 is fixedly connected in each placement box 420, a spindle of the third motor 460 is vertically arranged, a bearing plate 461 is coaxially and fixedly connected on the spindle of the third motor 460, a plurality of bearing ports are formed in the bearing plate 461, the bearing ports are arranged at intervals by taking a rotation axis of the bearing plate 461 as a circle center, and one sample tube can be placed in each bearing port. The setting of a plurality of bearing mouths is utilized, improves the volume of placing of sample pipe, and when detecting the cell sample in the sample pipe, utilizes third motor 460 drive carrier disk 461 to rotate, can make the sample pipe on a plurality of bearing mouths remove the below to sampling needle 332 one by one to the realization is sample one by one to a plurality of sample pipes.
Referring to fig. 3, 6 and 7, the detection box 100 is provided with a cleaning mechanism 500, the cleaning mechanism 500 comprises a water storage bottle 510 fixedly connected to one side of the detection box 100, a water outlet pipe 520 is communicated with the water storage bottle 510, the water outlet pipe 520 is located at a position close to the bottom of the water storage bottle 510, one end of the water outlet pipe 520, far away from the water storage bottle 510, stretches into the detection box 100 and then is communicated with a butt joint pipe 530, the butt joint pipe 530 is vertically arranged, the butt joint pipe 530 is in sliding connection with the inside of the detection box 100, a power assembly 540 is installed in the detection box 100, and the power assembly 540 is used for driving the butt joint pipe 530 to slide. The air supply tank 200 is provided with the water pressing assembly 550, the water pressing assembly 550 comprises an air supply pipe 551 communicated with the air supply tank 200, one end of the air supply pipe 551, far away from the air supply tank 200, is communicated with a control valve 552, the control valve 552 is a three-way valve, an air inlet of the control valve 552 is communicated with the air supply pipe 551, one air outlet of the control valve 552 is communicated with a first air pipe 553, and one end, far away from the control valve 552, of the first air pipe 553 extends into the water storage bottle 510 from the top end of the water storage bottle 510 and is communicated with the water storage bottle 510. The other air outlet of the control valve 552 is communicated with a second vent pipe 554, and one end of the second vent pipe 554 away from the control valve 552 is communicated with the inside of the butt joint pipe 530.
Referring to fig. 6 and 7, the power assembly 540 includes a second motor 541 fixedly coupled to the inside of the detection box 100, a main shaft of the second motor 541 is vertically disposed, a support plate 542 is fixedly coupled to the main shaft of the second motor 541, the support plate 542 is horizontally disposed, the support plate 542 horizontally rotates with an axis of the main shaft of the second motor 541 as a center of a circle, and the docking tube 530 is fixedly coupled to the support plate 542. When one cell sample is detected, the electric control valves 442 on the two communicating pipes 441 are closed, then the control valves 552 are controlled to be opened, the air supply pipe 551 is communicated with the first ventilating pipe 553 through the control valves 552, meanwhile, the second motor 541 is started, the main shaft of the second motor 541 rotates to drive the supporting plate 542 to horizontally rotate, the butt joint pipe 530 is aligned with the sampling needle 332, then the electric push cylinder 331 is started, the sampling needle 332 is driven to be inserted into the butt joint pipe 530, the sampling needle 332 is sealed and butted with the butt joint pipe 530, then the second cylinder 211 drives the lower pressing plate 212 to move downwards, so that the lower pressing plate 212 can press the air in the air supply box 200 into the water storage bottle 510, and then the cleaning water in the water storage bottle 510 is pressed into the butt joint pipe 530 through the water outlet pipe 520, and then can enter the sampling pipe 333 through the sampling needle 332, so that the inside cleaning of the sampling pipe 333 and the spray nozzle 310 is realized; after the sampling tube 333 and the nozzle 310 are cleaned, the control valve 552 is adjusted to enable the air supply tube 551 to be communicated with the second air vent tube 554, then the pressing plate 212 is continuously pressed down, so that air can be supplied to the sampling tube 333, residual moisture in the sampling tube 333 and the nozzle 310 is blown off and dried by utilizing air flow, the cleaning and drying speed of the sampling tube 333 is improved, and the detection efficiency of the cell sample is improved.
The implementation principle of the flow cytometer of the embodiment of the application is as follows: when detecting a cell sample, first air cylinder 451 is started first, sampling port 421 on placing box 420 is opened, then the sample to be detected is placed into placing box 420 by staff, then sampling port 421 is closed, then second air cylinder 211, second air cylinder 211 drives down platen 212, air in air feed box 200 is fed into placing box 420 through communicating pipe 441 by lower platen 212, air environment in placing box 420 is consistent with air environment in detecting box 100, then first motor 431 is started, supporting plate 410 is driven by first motor 431 to move, placing box 420 with sample tube is moved into detecting box 100, sampling port 421 is opened again, then sampling needle 332 can detect the cell sample in the sample tube, at this time, another placing box 420 is moved out from detecting box 100 under the driving of supporting plate 410, when detecting the cell sample in one of placing boxes 420, the sample cell in another placing box 420 can be simultaneously taken out, and the new sample tube is directed, and the detecting efficiency of the cell sample tube is improved.
The above embodiments are not intended to limit the scope of the present application, so: all equivalent changes in structure, shape and principle of the application should be covered in the scope of protection of the application.
Claims (10)
1. A flow cytometer, characterized in that: the device comprises a detection box (100), an air supply box (200) and a sample injection mechanism (400), wherein the sample injection mechanism (400) comprises a pushing assembly (430) and a placement box (420) for containing a sample tube, the placement box (420) is slidably connected to the detection box (100), the pushing assembly (430) is connected with the placement box (420), and the pushing assembly (430) is used for driving the placement box (420) to slide into the detection box (100); the placement box (420) is communicated with the air supply box (200), and the air supply box (200) is used for supplying constant temperature and humidity air into the placement box (420).
2. A flow cytometer as described in claim 1, wherein: sample introduction mechanism (400) still includes carrier plate (410), carrier plate (410) wear to establish on detection case (100), carrier plate (410) sliding connection is in detection case (100), push assembly (430) with carrier plate (410) are connected in order to drive carrier plate (410) reciprocal sliding, place case (420) are provided with two, two place case (420) all fixed connection are in on carrier plate (410), two place case (420) are in the drive of carrier plate (410) is gone into alternately in detection case (100), two place case (420) all with air feed case (200) intercommunication.
3. A flow cytometer as described in claim 2, wherein: be provided with liquid flow mechanism (300) in detection case (100), liquid flow mechanism (300) include shower nozzle (310), sheath liquid supply module (320) and sample extraction module (330), shower nozzle (310) fixed connection is in detection case (100), sheath liquid supply module (320) are connected on detection case (100), sheath liquid supply module (320) are used for to shower nozzle (310) supply sheath liquid, sample extraction module (330) include sample needle (332), sampling tube (333) and electricity push away jar (331), electricity push away jar (331) fixed connection is in detection case (100), sample needle (332) fixed connection is in on the piston rod of electricity push away jar (331), electricity push away jar (331) drive sample needle (332) go up and down so that sample needle (332) inserts in the sample, sampling tube (333) with sample needle (332) intercommunication, sample tube (333) keep away from sample needle (332) one end with shower nozzle (310) intercommunication.
4. A flow cytometer as described in claim 3, wherein: the air feed box (200) is the constant temperature incubator, be provided with air feed subassembly (210) and two sets of communication subassembly (440) on air feed box (200), two sets of communication subassembly (440) and two place case (420) one-to-one setting, every group communication subassembly (440) all include communicating pipe (441) and automatically controlled valve (442), one end of communicating pipe (441) with inside air feed box (200), the other end of communicating pipe (441) rather than corresponding place case (420) intercommunication, the setting of automatically controlled valve (442) is in communicating pipe (441), automatically controlled valve (442) are used for controlling opening and shutting of communicating pipe (441), air feed subassembly (210) be used for with air feed in air feed box (200) in communicating pipe (441).
5. The flow cytometer of claim 4, wherein: every sample connection (421) has all been seted up at the top of placing case (420), be provided with shutoff piece (450) on placing case (420), shutoff piece (450) all include first cylinder (451) and shutoff board (452), first cylinder (451) fixed connection place on case (420), shutoff board (452) sealing sliding connection place in case (420), the piston rod of first cylinder (451) with shutoff board (452) fixed connection, first cylinder (451) drive shutoff board (452) slide in order to right opening and closing of sample connection (421).
6. The flow cytometer of claim 4, wherein: the detection box (100) is provided with a cleaning mechanism (500), the cleaning mechanism (500) comprises a water storage bottle (510), a water outlet pipe (520), a butt joint pipe (530), a water pressing component (550) and a power component (540), the water storage bottle (510) is fixedly connected to the detection box (100), the butt joint pipe (530) is slidably connected to the detection box (100), the butt joint pipe (530) is communicated with the water storage bottle (510) through the water outlet pipe (520), the water pressing component (550) is connected to the Chu Shuiping (510), the water pressing component (550) is used for pressing cleaning water in the water storage bottle (510) into the butt joint pipe (530), the power component (540) is connected to the detection box (100), and the power component (540) is connected to the butt joint pipe (530) to drive the butt joint pipe (530) to slide.
7. A flow cytometer as described in claim 6, wherein: the water pressing assembly (550) comprises an air supply pipe (551), a control valve (552) and a first vent pipe (553), the air supply pipe (551) is communicated with the inside of the air supply box (200), the air supply pipe (551) is far away from one end of the air supply box (200) and is communicated with the control valve (552), the first vent pipe (553) is communicated with an air outlet of the control valve (552), and one end of the first vent pipe (553) far away from the control valve (552) is led into the inside of the water storage bottle (510) from the top of the water storage bottle (510).
8. A flow cytometer as described in claim 7, wherein: the control valve (552) is a three-way valve, the first vent pipe (553) is communicated with one air outlet of the control valve (552), the other air outlet of the control valve (552) is communicated with a second vent pipe (554), and one end, far away from the control valve (552), of the second vent pipe (554) is communicated with the butt joint pipe (530).
9. A flow cytometer as described in claim 6, wherein: the power assembly (540) comprises a second motor (541) and a support plate (542), the second motor (541) is fixedly connected in the detection box (100), the support plate (542) is fixedly connected to a main shaft of the second motor (541), and the butt joint pipe (530) is fixedly connected to the support plate (542).
10. A flow cytometer as described in claim 3, wherein: a first one-way valve (422) is arranged on each placement box (420), and the first one-way valve (422) enables air in the placement boxes (420) to flow outwards from the interiors of the placement boxes (420) in a one-way mode.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310868071.2A CN116698710A (en) | 2023-07-14 | 2023-07-14 | Flow cytometer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310868071.2A CN116698710A (en) | 2023-07-14 | 2023-07-14 | Flow cytometer |
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| CN116698710A true CN116698710A (en) | 2023-09-05 |
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