CN212008253U

CN212008253U - Full-automatic flow cytometry

Info

Publication number: CN212008253U
Application number: CN202020671934.9U
Authority: CN
Inventors: 夏兆麟
Original assignee: Zhengzhou Qianmai Beikang Medical Laboratory Co ltd
Current assignee: Zhengzhou Qianmai Beikang Medical Laboratory Co ltd
Priority date: 2020-04-27
Filing date: 2020-04-27
Publication date: 2020-11-24
Anticipated expiration: 2030-04-27

Abstract

The utility model discloses a full-automatic flow cytometry relates to cell analysis equipment. The scheme is provided aiming at the problem that the analysis machine head and the cell sample need to be at different environmental temperatures in the prior art. A first air pump and a second air pump are arranged above the inspection bin, the first air pump is communicated with the cavity through a first air inlet pipe and an air suction pipe respectively, and a refrigerating mechanism is connected to the first air inlet pipe in series; an insulating layer is arranged in the cavity; the heat insulation layer surrounds the periphery of the analysis machine head, and a gap is reserved between the heat insulation layer and the analysis machine head; the lower end of the heat-insulating layer is provided with a through hole for exposing the probe head to the cavity body by the analyzer head; a second air inlet pipe is arranged in a gap between the heat preservation layer and the analysis machine head and communicated with a second air pump, and an exhaust pipe is further arranged and communicated with the atmosphere. The advantage is that the cell sample and the analysis head can be maintained at their respective required ambient temperatures by the first and second air pumps, thereby improving the accuracy of the analytical detection.

Description

Full-automatic flow cytometry

Technical Field

The utility model relates to a cell analysis equipment especially relates to full-automatic flow cytometry.

Background

With the development of science and technology, fully automatic flow cytometry is widely used in laboratories. The analyzer head in the analyzer belongs to a precision instrument, the working temperature cannot be too low, and the cell sample to be analyzed often needs to be kept in a low-temperature state. The test chamber in the analyzer is difficult to meet the temperature requirements of the two, so that the analysis result has larger deviation.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a full-automatic flow cytometry appearance realizes temperature regulation's in the inspection storehouse function and improves the analysis result.

The utility model discloses a full-automatic flow cytometry, including the inspection storehouse, be equipped with the cavity that is used for placing the cell sample in the inspection storehouse, the inspection storehouse still is equipped with the door that supplies the cell sample to pass in and out the cavity, is equipped with the analysis aircraft nose on the upper portion of cavity; a first air pump and a second air pump are arranged above the inspection bin, the first air pump is respectively communicated with the cavity through a first air inlet pipe and an air suction pipe, and a refrigerating mechanism is connected to the first air inlet pipe in series; an insulating layer is arranged in the cavity; the heat insulation layer surrounds the periphery of the analysis machine head, and a gap is reserved between the heat insulation layer and the analysis machine head; the lower end of the heat-insulating layer is provided with a through hole for exposing the probe head to the cavity body by the analyzer head; and a second air inlet pipe is arranged in a gap between the heat preservation layer and the analysis machine head and is communicated with the second air pump, and an exhaust pipe is also arranged and is communicated with the atmosphere.

The refrigerating mechanism is a water bath heat exchange mechanism.

The air suction pipe is communicated with one side of the cavity far away from the bin gate, and the first air inlet pipe is communicated with one side of the cavity near the bin gate.

And a pressure release valve is also arranged at the upper part of the cavity.

Full-automatic flow cytometry appearance, its advantage lie in, the second air pump can external heating installation or room temperature gas, send out the air that is higher than the inside temperature of cavity to the space that the heat preservation encircleed, make the analysis aircraft nose keep working under relatively higher temperature. And then the first air pump circulates cold air in the cavity to ensure that the cell sample can obtain relatively low temperature to prevent denaturation. Therefore, the cell sample and the analysis machine head can control the respective environmental temperature through the corresponding air pump, so that the analysis and detection precision is improved.

Drawings

Fig. 1 is the structure schematic diagram of the full-automatic flow cytometer of the present invention checks the bin.

Reference numerals: 10-inspection bin, 11-cavity, 12-pressure release valve and 13-bin gate; 20-analyzer head, 21-insulating layer; 31-a first air pump, 32-a second air pump, 33-a refrigeration mechanism; 41-first inlet pipe, 42-second inlet pipe, 43-suction pipe and 44-exhaust pipe.

Detailed Description

As shown in FIG. 1, full-automatic flow cytometry, including inspection storehouse 10, be equipped with the cavity 11 that is used for placing the cell sample in inspection storehouse 10, inspection storehouse 10 still is equipped with the door 13 that supplies the cell sample to advance cavity 11, is equipped with analysis aircraft nose 20 on the upper portion of cavity 11. A first air pump 31 and a second air pump 32 are arranged above the inspection chamber 10, the first air pump 31 is respectively communicated with the cavity 11 through a first air inlet pipe 41 and an air suction pipe 43, and the first air inlet pipe 41 is also connected with a refrigerating mechanism 33 in series. The cavity 11 is internally provided with a heat preservation layer 21. The insulating layer 21 surrounds the periphery of the analysis head 20, and a gap is reserved between the insulating layer and the analysis head 20. The lower end of the insulating layer 21 is provided with a through hole for exposing the probe head 20 to the cavity 11. A second air inlet pipe 42 is arranged in the gap between the heat preservation layer 21 and the analysis head 20 and is communicated with the second air pump 32, and an exhaust pipe 44 is also arranged and is communicated with the atmosphere. The first air pump 31 and the second air pump 32 are both fixed with the upper end surface of the inspection chamber 10 through shock-proof pads. The upper end of the heat preservation layer 21 is hermetically connected with the upper end face of the cavity 11, a temperature partition plate is arranged at the lower end of the heat preservation layer 21, and the through hole is formed in the middle of the temperature partition plate. The analyzer head 20 is fixed on the lower end face of the cavity 11 through a mounting seat and is located in a space surrounded by the insulating layer 21.

The refrigerating mechanism 33 is a water bath heat exchange mechanism. The pipeline vibration can be avoided to the utmost extent, and heat exchange efficiency is enough to satisfy the experimental requirement, and it is very convenient that the laboratory environment provides the water-bath heating moreover.

The air suction pipe 43 is communicated with one side of the cavity 11 far away from the door 13, and the first air inlet pipe 41 is communicated with one side of the cavity 11 near the door 13. During the cool air circulation, a temperature difference region is necessarily formed in the cavity 11. The position of the first air inlet pipe 41 communicated with the cavity 11 is close to the bin door 13 as much as possible, so that the original temperature environment can be recovered to the maximum extent after the bin door is opened and then closed.

And a pressure release valve 12 is also arranged at the upper part of the cavity 11. Prevent the occurrence of accidents caused by the increase of the pressure formed in the cavity 11 by the circulation of the cold air.

Full-automatic flow cytometer's theory of operation as follows, at first with the gas in the first intake pipe 41 of the mode cooling of refrigeration mechanism 33 through the water bath. Cold gas is then circulated within the chamber 11 by means of the first air pump 31 to try to meet the temperature requirements of the cell sample. Meanwhile, air with the temperature higher than that of the cavity 11 is blown into the area surrounded by the heat preservation layer 21 through natural air suction of the laboratory environment or an external heating pipeline through the second air pump 32, so that the analysis machine head 20 is prevented from being greatly cooled. Under the condition that the corresponding temperatures inside and outside the heat preservation layer 21 respectively meet the test requirements, the bin door 13 can be opened to put in a cell sample for detection operation.

Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes are intended to fall within the scope of the claims.

Claims

1. The full-automatic flow cytometry comprises a detection bin (10), wherein a cavity (11) for placing a cell sample is arranged in the detection bin (10), the detection bin (10) is also provided with a bin gate (13) for the cell sample to enter and exit the cavity (11), and the upper part of the cavity (11) is provided with an analysis machine head (20); the air pump device is characterized in that a first air pump (31) and a second air pump (32) are arranged above the inspection bin (10), the first air pump (31) is communicated with the cavity (11) through a first air inlet pipe (41) and an air suction pipe (43), and the first air inlet pipe (41) is also connected with a refrigerating mechanism (33) in series; an insulating layer (21) is arranged in the cavity (11); the heat insulation layer (21) surrounds the periphery of the analysis machine head (20), and a gap is reserved between the heat insulation layer and the analysis machine head (20); the lower end of the heat-insulating layer (21) is provided with a through hole for exposing the probe of the analysis head (20) to the cavity (11); a second air inlet pipe (42) is arranged in a gap between the heat preservation layer (21) and the analysis machine head (20) and communicated with the second air pump (32), and an exhaust pipe (44) is arranged and communicated with the atmosphere.

2. The fully automated flow cytometer as described in claim 1, wherein said cooling mechanism (33) is a water bath heat exchanger.

3. The fully automatic flow cytometer as described in claim 1, wherein the air suction tube (43) is connected to the side of the chamber (11) away from the door (13), and the first air inlet tube (41) is connected to the side of the chamber (11) close to the door (13).

4. A fully automatic flow cytometer as described in claim 1, wherein said chamber (11) is further provided with a pressure relief valve (12) at the upper portion thereof.