CN116590314A - Gene for improving salt and alkali tolerance of wheat and application thereof - Google Patents
Gene for improving salt and alkali tolerance of wheat and application thereof Download PDFInfo
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- 150000003839 salts Chemical class 0.000 title abstract description 17
- 241000196324 Embryophyta Species 0.000 claims abstract description 33
- 239000002773 nucleotide Substances 0.000 claims abstract description 5
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 5
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- 230000035784 germination Effects 0.000 claims description 8
- 239000013604 expression vector Substances 0.000 claims description 3
- 230000007226 seed germination Effects 0.000 abstract description 4
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- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8267—Seed dormancy, germination or sprouting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
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Abstract
The invention discloses a gene for improving the salt and alkali tolerance of wheat and application thereof, wherein the gene has a nucleotide sequence shown as SEQ ID NO. 3. The invention provides a gene derived from wheat, which can improve the saline-alkali tolerance of plants, and the embodiment of the invention proves that the seed germination rate of plants transformed with the gene is obviously improved in a saline-alkali environment, thus providing a new effective choice for improving the saline-alkali tolerance of plants and having good application prospect.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a gene for improving the saline-alkali tolerance of wheat and application thereof.
Background
Soil salinization is a major problem facing the world, and has a great influence on the growth, yield and quality of crops, and the sustainable development of modern agriculture is greatly limited. Improving the saline-alkali soil, cultivating the saline-alkali resistant variety and enhancing the saline-alkali resistance are effective measures for fully utilizing the saline-alkali soil. The improvement of the saline-alkali soil comprises a physical measure improvement method, a hydraulic engineering measure improvement method, a chemical improvement method, a biological improvement method and the like. However, various comprehensive treatment technologies have advantages and disadvantages, such as high cost and weak sustainability of a physical method; the chemical method has soil secondary pollution and certain limitation; the repairing time period required by the biological method is long, etc. In addition to comprehensive treatment by using traditional physical, chemical, biological and other measures, the application of the latest molecular biology method for improving crop tolerance through genetic engineering is one of the most economical and effective methods.
The saline-alkali soil contains excessive NaCl and Na 2 SO 4 、Na 2 CO 3 And NaHCO 3 And the like. The toxicity of saline-alkali soil to plants mainly comprises compound toxicity caused by the interaction of salt stress and high pH stress. The main damage caused by saline-alkali stress to plants is represented in three aspects: firstly, a great amount of metal ions (mainly Na) in cytoplasm are accumulated, so that the ion balance in cells is destroyed, the physiological and biochemical metabolic processes in cells are inhibited, the photosynthesis capacity of plants is reduced, and finally the plants die due to carbon starvation; secondly, the saline-alkali soil is a hypertonic environment, and can prevent plant roots from absorbing moisture, so that plants die due to drought; thirdly, the pH value of the saline-alkali soil is higher, so that the plant body is unbalanced with the acid and alkali in the external environment, and thenDestroying the structure of the cell membrane, causing the extravasation of intracellular lysates and death of the plant. Thus, saline-alkali stressed plants on the one hand reduce ion accumulation in the cytoplasm and on the other hand produce certain specific products such as proteins, amino acids, saccharides and the like through the accumulation process to enhance the osmotic pressure of cells, prevent the water loss of cells and stabilize the structures of plasma membranes and enzymes.
The Chinese patent CN101962656B discloses the application of the TT1 gene in improving the salt and alkali tolerance of plants, and experiments prove that the seed germination rate of plants transformed with the TT1 gene is obviously improved in a salt and alkali environment, the proline content in the plants after growth is also improved, and the TT1 gene can be proved to be capable of effectively improving the salt and alkali tolerance of the plants.
Chinese patent application No. CN114711130A discloses a method for improving salt and alkali resistance of wheat by using Quinoa Polysaccharide (QPS) solution to treat wheat seeds or wheat seedlings growing in a salt environment or an alkali environment, soaking the wheat seeds, and spraying leaves or root irrigation to the wheat seedlings. After the wheat seeds or wheat seedlings growing in salt stress or alkali stress are treated by the QPS solution, the inhibition of the salt/alkali stress on the growth of the wheat seeds and the wheat seedlings is relieved, the germination rate and the germination potential of the wheat seeds in a salt/alkali environment are improved, the root length, the bud length, the dry weight and the fresh weight of the wheat seedlings and the wheat seeds are obviously improved, and various indexes of the wheat growth in a normal environment can be achieved.
Although the prior art has reported some methods for improving the saline-alkali tolerance of plants, how to further excavate more genes for improving the saline-alkali tolerance of wheat is still a technical problem to be solved by the person skilled in the art.
Disclosure of Invention
Based on the reasons, the invention provides a gene for improving the salt and alkali tolerance of wheat and application thereof.
Specifically, in order to achieve the purpose of the present invention, the present invention adopts the following technical scheme:
the invention relates to a gene for improving the salt and alkali tolerance of wheat, which is characterized by having a nucleotide sequence shown as SEQ ID NO. 3.
Another aspect of the invention relates to a plant expression vector comprising the above gene sequence.
The invention also relates to application of the gene in improving the saline-alkali tolerance of plants.
In a preferred embodiment of the present invention, the plant includes, but is not limited to, wheat, arabidopsis, etc., and the enhancing saline-alkali tolerance of the plant includes, but is not limited to, enhancing germination rate of the seed.
The beneficial effects of the invention are as follows:
the invention provides a gene derived from wheat, which can improve the saline-alkali tolerance of plants, and experiments prove that the seed germination rate of plants transformed with the gene is obviously improved in a saline-alkali environment, so that new effective selection is provided for improving the saline-alkali tolerance of plants, and the application prospect is good.
Drawings
FIG. 1 is a diagram showing agarose electrophoresis detection of a target gene;
FIG. 2 is a diagram showing agarose electrophoresis detection of plasmid DNA containing a target gene;
FIG. 3 is a graph showing experimental results of the effect of NaCl at different concentrations on the germination rate of Arabidopsis seeds.
Detailed Description
For a further understanding of the present invention, the technical aspects of the present invention will be clearly and fully described below in connection with the following examples, and it is apparent that the described examples are only some, but not all, examples of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Unless otherwise specified, all reagents involved in the examples of the present invention are commercially available products and are commercially available.
Example 1: gene acquisition and analysis
(1) The spike cDNA of Jimai 22, which grows at the highest speed in the experimental base of Shiqiangcun in the development area of the coastal economic technology of Weifang, shandong province, is used as a template, and the following primers are used for PCR amplification.
Upstream primer (SEQ ID NO: 1): 5'-caaagaaagctcgaccgtgtc-3' the number of the individual pieces of the plastic,
downstream primer (SEQ ID NO: 2): 5'-catgcaattttctacttctgtcg-3'.
(2) The PCR products were subjected to gel electrophoresis on 1% agarose gel, the electrophoresis results are shown in FIG. 1, in which lane 1 is the amplified product and M is the DNA Marker.
(3) And (3) performing gel cutting recovery on the target strip, cloning the target strip into a PT19-T vector, and sequencing to obtain the complete gene, wherein the nucleotide sequence is shown as SEQ ID NO.3, and the total length of the gene sequence is 1606bp.
Example 2: obtaining transgenic Arabidopsis plants and seeds
(1) According to SEQ ID NO:3, performing PCR amplification by using the scion cDNA of Jimai 22 as a template
Upstream primer (SEQ ID NO: 4): 5'-taatctctagaggcgcagaggtacctcaacctc-3' (containing the restriction enzyme XbaI site),
downstream primer (SEQ ID NO: 5): 5'-atgcgagctctccacgtcaacgcgcacgtcctg-3' (containing the restriction endonuclease Sac1 site).
(2) The PCR product was purified (see Qiagen PCR product purification kit) and then digested with XbaI and Sac1, recovered in a gel, and ligated with vector pBI121 (ligation site: xbaI and Sac 1) to give a DNA fragment comprising the sequence of SEQ ID NO:3, and an over-expression recombinant plasmid. The sequence comprising SEQ ID NO:3, transferring the over-expression recombinant plasmid into agrobacterium, and transforming arabidopsis by using an inflorescence dip-dyeing method.
(3) Picking up a nucleic acid sequence comprising SEQ ID NO:3, inoculating agrobacterium of the over-expression recombinant plasmid into LB liquid culture medium containing 20mg/L Str,50mg/LKan and 40mg/L Rif, collecting thalli after shaking at 28 ℃ for overnight, and re-suspending the thalli in MS liquid culture medium containing 0.01% of surfactant silwet-77 until the OD600 = 0.4-0.6, shaking at 28 ℃ for 1-2h, and standing for later use.
(4) Inflorescences grown from arabidopsis thaliana cultivated for 60 days are cut off and purified by a method comprising the steps of: 3, soaking inflorescences in agrobacterium solution of the over-expression recombinant plasmid for 2 minutes, then carrying out dark culture for 48 hours, transferring the arabidopsis seedlings after the dark culture into a normal illumination environment for growth, and obtaining the pods grown out later as transgenic T0 generation seeds.
(5) Identification of transgenes: planting the harvested seeds, taking a few leaves after the seeds grow to 50 days, and carrying out PCR detection by adopting the following primers
Upstream primer (SEQ ID NO: 6): 5'-tcgtcgccggcttcctgctcgtg-3'
Downstream primer (SEQ ID NO: 7): 5'-tacacaccaccttgccgttgtc-3'
Then agarose electrophoresis is carried out to detect whether a target band appears, if so, the target gene is transferred into Arabidopsis. The detection result is shown in fig. 2:
1-12 lanes are transgenic arabidopsis genome DNA, and M lanes are DNA containing SEQ ID NO:3, the target band of the DNA to be detected is consistent with the band of the over-expressed plasmid DNA, and the target band is expressed as SEQ ID NO:3 nucleotide over-expression transgenic positive plants and seeds thereof are prepared.
(6) After the transgenic positive plants are mature, seeds are collected for standby. Similarly, SEQ ID NO:3, preparing an Arabidopsis plant with over-expressed sequence and preparing seeds for later use.
Example 3: effect of different concentrations of NaCl on Arabidopsis seed germination Rate
The salt of saline-alkali soil is usually NaCl, na 2 SO、Na 2 CO and NaHCO 3 And the like, salt stress can cause water potential reduction and ion stress caused by Na ion increase, and the absorption of K ions, ca ions and other nutrients by plants is influenced, so that the plants are damaged. Thus, the present invention employs NaCl to simulate salt stress conditions.
The prepared MS culture medium is respectively added with different amounts of NaCl before sterilization, so that the final concentration of NaCl is respectively 0mmol/L (control group), 50mmol/L, 100mmol/L, 150mmol/L, 200mmol/L, 250mmol/L and 300mmol/L, and the culture medium is packaged in culture dishes after high-pressure steam sterilization. After the culture medium is solidified, 2mL of sterile water is used for suspending the seeds, the seeds are transferred into the culture medium, after the seeds are uniformly sown, the excessive sterile water is removed, a dish cover is opened, the dish cover is placed in a sterile environment for 1h until the surface is dried, the dish cover is sealed, then the dish cover is placed into a culture room (22 ℃ C. With illumination intensity of 6000-8000 lx,16h/8h light-dark period and relative humidity of 70%) for culture, 3 repetitions are carried out on each treatment, the germination number is counted after 7 days, and the average value is obtained.
The experimental results are shown in FIG. 3, and the experimental results show that the non-transgenic Arabidopsis seeds are sensitive to the change of the concentration of NaCl and basically do not sprout even at 250mM and 300 mM; the transgenic arabidopsis seeds have better tolerance to NaCl, and the final germination rate of the seeds is higher at the concentrations of 50mM, 100mM and 150mM, and even at the concentrations of 200mM, 250mM and 300mM, the germination rate still remains certain, and is obviously higher than that of non-transgenic seeds.
The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations to the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.
Claims (5)
1. A gene, characterized in that the gene has a nucleotide sequence shown in SEQ ID No. 3.
2. A plant expression vector comprising the gene sequence of claim 1.
3. Use of the gene of claim 1 or the plant expression vector of claim 2 for increasing the saline-alkali tolerance of a plant.
4. The use according to claim 3, wherein the plant is wheat or arabidopsis.
5. The use of claim 4, wherein said increasing the saline-alkali tolerance of the plant comprises increasing the germination rate of the seed.
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| CN202310844473.9A CN116590314B (en) | 2023-07-11 | 2023-07-11 | Gene for improving salt and alkali tolerance of wheat and application thereof |
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| CN202310844473.9A CN116590314B (en) | 2023-07-11 | 2023-07-11 | Gene for improving salt and alkali tolerance of wheat and application thereof |
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| CN116590314B CN116590314B (en) | 2023-09-29 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101509006A (en) * | 2009-03-19 | 2009-08-19 | 河南大学 | Uses of arabidopsis thaliana gene AIK1 in regulating and controlling vegetation growth, and in intimidation resistance aspect |
| CN101962656A (en) * | 2009-07-23 | 2011-02-02 | 四川贝安迪生物基因工程有限公司 | Application of TT1 gene in improving saline alkali tolerance of plants |
| CN102021179A (en) * | 2009-11-25 | 2011-04-20 | 北京未名凯拓作物设计中心有限公司 | Application of rice gene KT484 to improvement of stress tolerance performance of plants |
| CN102584961A (en) * | 2012-02-28 | 2012-07-18 | 中国农业科学院作物科学研究所 | Anti-insect protein Cry1A.401 and expression vector and application thereof |
| CN104561053A (en) * | 2015-01-07 | 2015-04-29 | 山东大学 | Wheat salt-tolerant gene TaBASS2 and application thereof |
| CN107058348A (en) * | 2016-12-13 | 2017-08-18 | 江苏省农业科学院 | A kind of wheat cdna for improving plant scab resistance and its application |
| US20230193307A1 (en) * | 2020-02-05 | 2023-06-22 | Agrisea Corporation | Methods and compositions for production of saline tolerant plants |
-
2023
- 2023-07-11 CN CN202310844473.9A patent/CN116590314B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101509006A (en) * | 2009-03-19 | 2009-08-19 | 河南大学 | Uses of arabidopsis thaliana gene AIK1 in regulating and controlling vegetation growth, and in intimidation resistance aspect |
| CN101962656A (en) * | 2009-07-23 | 2011-02-02 | 四川贝安迪生物基因工程有限公司 | Application of TT1 gene in improving saline alkali tolerance of plants |
| CN102021179A (en) * | 2009-11-25 | 2011-04-20 | 北京未名凯拓作物设计中心有限公司 | Application of rice gene KT484 to improvement of stress tolerance performance of plants |
| CN102584961A (en) * | 2012-02-28 | 2012-07-18 | 中国农业科学院作物科学研究所 | Anti-insect protein Cry1A.401 and expression vector and application thereof |
| CN104561053A (en) * | 2015-01-07 | 2015-04-29 | 山东大学 | Wheat salt-tolerant gene TaBASS2 and application thereof |
| CN107058348A (en) * | 2016-12-13 | 2017-08-18 | 江苏省农业科学院 | A kind of wheat cdna for improving plant scab resistance and its application |
| US20230193307A1 (en) * | 2020-02-05 | 2023-06-22 | Agrisea Corporation | Methods and compositions for production of saline tolerant plants |
Non-Patent Citations (1)
| Title |
|---|
| ZHENWEI YAN等: "MPK3/6-induced degradation of ARR1/10/12 promotes salt tolerance in Arabidopsis", 《EMBO REP .》, vol. 22, no. 10, pages 52457 * |
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| CN116590314B (en) | 2023-09-29 |
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