CN116555000B

CN116555000B - Salmonella pathogen separation and purification equipment

Info

Publication number: CN116555000B
Application number: CN202310840706.8A
Authority: CN
Inventors: 李彦; 段笑笑; 付红蕾; 邴啟政; 马帅; 张严琦
Original assignee: Qingdao Animal Disease Control Center
Current assignee: Qingdao Animal Disease Control Center
Priority date: 2023-07-11
Filing date: 2023-07-11
Publication date: 2023-09-12
Anticipated expiration: 2043-07-11
Also published as: CN116555000A

Abstract

The invention provides salmonella pathogen separation and purification equipment, which relates to the technical field of salmonella detection and comprises a base, wherein the front side of the base is a picking and placing position, the left side of the base is a constant temperature culturing position, the rear side of the base is a selecting position, the right side of the base is a cleaning and sterilizing position, a mixing position is arranged between the picking and placing position and the constant temperature culturing position, a suction position is arranged between the constant temperature culturing position and the selecting position, an inoculation position is arranged between the selecting position and the cleaning and sterilizing position, a rotatable tray is mounted on the base, a placing opening for placing a vessel is circumferentially arranged on the tray, and an L-shaped top plate is fixedly connected above the base. The device can automatically complete the processes of mean mixing, bacteria increasing culture, suction, heat preservation culture, selection, inoculation and subsequent cleaning and sterilization by only arranging corresponding samples and various culture mediums on a tray according to time, and accurately identifying salmonella by adopting image identification equipment for purification culture.

Description

Salmonella pathogen separation and purification equipment

Technical Field

The invention relates to the technical field of salmonella detection, in particular to salmonella pathogen separation and purification equipment.

Background

Salmonella is a member of the genus Salmonella of the family Enterobacteriaceae, and is a class of gram-negative enterobacteria that are conditionally intracellular parasitic. The main pathogenic bacteria in cases of paratyphoid of pigs are salmonella choleraesuis and salmonella typhi. In addition, salmonella typhimurium, salmonella dublin, salmonella enteritidis, and the like also often cause this disease. Can cause salmonellosis of various animals, and the salmonella is a pathogenic bacterium with extremely wide natural distribution, and can be separated from almost all vertebrates and even insects.

Salmonella of domestic animals and birds mostly show enteritis and septicemia, and at the same time, salmonella of animals can infect human beings through food, and is a main bacterium causing human food poisoning, and human non-typhoid salmonella is the most common food-borne disease worldwide. Therefore, it is important to understand the clinical prevalence of salmonella at present, and when salmonella is studied, it is first required to separate and purify salmonella from a sample.

At present, separation and purification are required to be carried out in a sterile laboratory, a large number of tools and equipment are required to be prepared, the steps of homogenizing mixing, sucking, inoculating, selecting and the like of samples are all required to be carried out manually, the operation is complicated, especially, in the selection link, most of salmonella or similar colonies are selected from a culture medium by naked eyes, the shape, color and other parameters of the colonies are generally required to be observed, and the mistakes are easy to select.

Disclosure of Invention

Aiming at the problems, the invention provides salmonella pathogen separation and purification equipment, which can automatically complete the processes of mean mixing, enrichment culture, suction, heat preservation culture, selection, inoculation and subsequent cleaning and sterilization by only arranging corresponding samples and various culture mediums on a tray according to time, and accurately identifying salmonella by adopting image identification equipment for purification culture.

In order to solve the problems, the invention adopts the following technical scheme:

the utility model provides a salmonella pathogen separation purification equipment, includes the base, the front side of base is for getting to put the position, and the left side is for the constant temperature and cultivates the position, and the rear side is for selecting the position of disinfecting, it is mixed the position to get to put between position and the constant temperature and cultivate the position, it is to absorb the position to cultivate the position with selecting between the position to constant temperature, it is inoculation position to select between the position and the position of disinfecting to wash, install rotatable tray on the base, tray circumference is equipped with the mouth of placing that is used for placing the household utensils, the top fixedly connected with L type roof of base, rotatable carousel is installed to the below of L type roof, the top of carousel corresponds mixed position, absorbs position and inoculation position department equal fixed mounting have first electric telescopic handle, and three correspond mixed position, absorb position and inoculation position department the flexible end of first electric telescopic handle is installed respectively and is absorbed subassembly and is fixed a position inoculation subassembly, be equipped with a plurality of arc mouths on the carousel circumference, image recognition equipment that corresponds the arc mouthful is installed at the top of L type roof.

Preferably, the base is connected with a lower driving shaft in a rotating way, the upper end of the lower driving shaft is fixedly connected with the center of the tray, the top of the L-shaped top plate is connected with an upper driving shaft in a rotating way, and the lower end of the upper driving shaft is fixedly connected with the center of the turntable.

Preferably, the base is rotatably connected with a rotating shaft at a constant temperature culturing position, the upper end of the rotating shaft is fixedly connected with a material tray positioned between the turntable and the tray, the constant temperature culturing position is also provided with an arc-shaped heat preservation cover covering the left half material tray, the inner wall of the arc-shaped heat preservation cover is provided with an arc-shaped heating plate, the lower driving shaft, the upper driving shaft and the rotating shaft are driven by corresponding first motors, and the constant temperature culturing position is also provided with a loading and unloading mechanism.

Preferably, the feeding and discharging mechanism comprises a plurality of annular electromagnets fixedly installed at the bottom of the material tray in the circumferential direction, an iron ring matched with the annular electromagnets is sleeved at the rear part of the dish, a second electric telescopic rod is fixedly connected to the position, at the constant temperature, of the base, and a supporting plate capable of jacking the dish is fixedly connected to the telescopic end of the second electric telescopic rod.

Preferably, the mixing assembly and the positioning inoculation assembly comprise a second motor fixedly installed at the telescopic end of the corresponding first electric telescopic rod, the mixing assembly further comprises a stirring rod fixedly installed at the output end of the corresponding second motor, and stirring blades are installed at the bottom of the stirring rod.

Preferably, the positioning inoculation assembly comprises an electric sliding rail fixedly arranged at the output end of the corresponding second motor, and an inoculation ring is slidably arranged at the bottom of the electric sliding rail.

Preferably, the suction assembly comprises a liquid suction pipe fixedly connected to the telescopic end corresponding to the first electric telescopic rod, a piston is elastically connected in the liquid suction pipe through a spring, and a liquid suction head is arranged at the bottom of the liquid suction pipe.

Preferably, the top lateral wall fixedly connected with annular plate of L type roof, be located on the annular plate and mix the position, absorb the position, location inoculation position and wash the position department of disinfecting and all install electrically conductive seat, three electrically operated telescopic handle's top all fixed mounting have with electrically conductive seat complex electrically conductive head, second motor, electronic slide rail and spring all with the electrically conductive head electric connection of corresponding position.

Preferably, the controller is installed at the top of L type roof, image recognition device includes camera and treater, the top at L type roof is installed to the camera, treater and controller electric connection, the electrically conductive seat electric connection of controller and everywhere.

Preferably, the cleaning box is installed to the cleaning position department that disinfects, the both sides of cleaning box all are equipped with the opening that supplies carousel and tray to pass, the washing tank internal fixation has the washing tank that is located between tray and the carousel, but the pump of pump sterilization liquid is installed to the bottom of cleaning box, be connected with the drain pipe between the play water end of pump and washing tank, the stoving pipe is still installed to the cleaning box, the top of stoving pipe is equipped with a plurality of apopores, the top of cleaning box is equipped with the household utensils slot, household utensils slot internal rotation is connected with the wash pipe, the lateral wall and the top of wash pipe all are equipped with the brush roller, the third motor is installed at the interior top of cleaning box, through a pair of gear engagement between the output and the wash pipe of third motor, the lateral wall of drain pipe still is connected with the branch pipe, the end of branch pipe passes through rotary joint with the wash pipe lower extreme and docks, installs the valve in the branch pipe.

The beneficial effects of the invention are as follows:

(1) Through setting up to get and put the position, mix the position, cultivate the position with constant temperature, absorb the position, select the position, inoculate and wash the position of disinfecting, rethread lower drive shaft and last drive shaft drive tray and carousel rotation respectively, only need place the household utensils of loading sample or culture medium on the tray according to time respectively, can be automatic carry out mean mixing, increase fungus cultivation, absorb, keep warm cultivation, select, inoculate and follow-up washing sterilization process.

(2) Through installing mixing assembly, load sample and increase bacterial liquid and place in placing the mouth of placing of tray in the household utensils, when turning to the mixed position, start the first electric telescopic handle that corresponds the position and drive the puddler and descend to the household utensils in, start corresponding second motor, can drive the stirring leaf and rotate and accomplish the homogeneity mixing process.

(3) Through installing charging tray, arc heat preservation cover and last unloading mechanism, the sample after the homogeneity changes to the constant temperature and cultivates the position, perhaps when selectively separating and cultivate and inoculating and cultivate, also can change to the constant temperature and cultivate the position, only need start second electric telescopic handle and drive the layer board and shift up, and then hold in the palm the household utensils of waiting to cultivate and rise, the iron ring of this household utensils mouth corresponds the electromagnetic iron of position with the charging tray bottom and inhale, in the rethread charging tray changes the arc heat preservation cover, realizes the heat preservation and cultivates the process through inside arc hot plate.

(4) Through installing and absorbing the subassembly, the household utensils after increasing the fungus change to the position of absorbing, only need start the first electric telescopic link of corresponding position, drive the pipette insert in the household utensils, the controller control spring circular telegram shrink drives the piston and moves up, and then in inhaling the pipette with the fungus liquid through the imbibition head, change bismuth sulfite agar culture dish to the position of absorbing again, drip fungus liquid on the culture dish, realize automatic absorption and the drip process.

(5) Through installing and selecting the position, adopt image recognition equipment, utilize image recognition technique, connect the database, discern colony form, color isoparametric in the bismuth sulfite agar culture dish, compare with the salmonella parameter in the database, and then the accurate colony of selecting salmonella to send colony position information to the controller, inoculate the subassembly by the location of controller control and carry out the location.

(6) Through installing location inoculation subassembly, receive the signal of controller, start the first electric telescopic link of corresponding position, drive the inoculating loop and descend, rethread starts the second motor that corresponds and electric slide rail and accurately remove the inoculating loop to the position of salmonella bacterial colony, scrape the bacterial colony through the inoculating loop, later turn to the location inoculation position with new culture medium, can accomplish follow-up purification culture process.

(7) Through installing the washing case, the inoculating loop, the liquid suction pipe and the mixing assembly need wash after using and disinfect, only need to turn over the three to the washing incasement, be located the washing tank top, control first electric telescopic handle again and start, can insert the sterilizing liquid of washing tank with inoculating loop respectively, liquid suction pipe and mixing assembly, accomplish the washing sterilization process, realize quick drying process through the hot-blast realization that the stoving pipe blows off afterwards, the household utensils can be taken off and place in the household utensils slot, start the third motor, drive the wash pipe through a pair of gear and brush roller and rotate, accomplish the scrubbing process to the household utensils inner wall, open the valve simultaneously, the water pump is with the sterilizing liquid get into the wash pipe through the branch pipe in, the washing process of disinfecting is realized to the blowout afterwards.

In summary, the invention integrates the processes of mean mixing, bacteria increasing culture, suction, heat preservation culture, selection, inoculation, subsequent cleaning and sterilization and the like into an integrated device, and the integrated device can be used for realizing the functions by changing the original process of using a plurality of independent devices into the process of using the integrated separation and purification device, thereby reducing the occupied space of the device, simultaneously greatly freeing the operation difficulty of staff, improving the working efficiency, and reducing the problems of sample pollution and the like possibly caused by manual operation.

Drawings

FIG. 1 is a top view of a salmonella pathogen separation and purification apparatus of the present invention;

FIG. 2 is a top view of a tray according to the present invention;

FIG. 3 is a top view of a base according to the present invention;

FIG. 4 is a bottom view of a turntable according to the present invention;

FIG. 5 is a top view of a turntable according to the present invention;

FIG. 6 is a side view of a turntable according to the present invention;

FIG. 7 is a schematic view of the internal structure of the cleaning tank according to the present invention;

fig. 8 is a top view of a vessel according to the present invention.

In the figure: 1. a base; 2. a tray; 3. a placement port; 4. a turntable; 5. an arc-shaped opening; 6. an L-shaped top plate; 7. a controller; 8. an image recognition device; 9. a conductive base; 10. an annular plate; 11. a cleaning box; 12. a brush roller; 13. a vessel slot; 14. a material tray; 15. an arc-shaped heat-preserving cover; 16. a vessel; 17. an iron ring; 18. a first electric telescopic rod; 19. a conductive head; 20. an upper drive shaft; 21. a lower drive shaft; 22. a supporting plate; 23. a second electric telescopic rod; 24. a rotating shaft; 25. an arc-shaped heating plate; 26. an annular electromagnet; 27. a second motor; 28. an electric slide rail; 29. an inoculating loop; 30. a stirring rod; 31. a pipette; 32. a spring; 33. a piston; 34. a liquid suction head; 35. an opening; 36. a third motor; 37. a gear; 38. a branch pipe; 39. a cleaning tank; 40. a liquid outlet pipe; 41. a water pump; 42. a flushing pipe; 43. and (5) drying the tube.

Detailed Description

In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit or scope of the invention, which is therefore not limited to the specific embodiments disclosed below.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Referring to fig. 1-8, a salmonella pathogen separation and purification device comprises a base 1, a picking and placing position is arranged on the front side of the base 1, a constant temperature culturing position is arranged on the left side, a selecting position is arranged on the rear side, a cleaning and sterilizing position is arranged on the right side, a mixing position is arranged between the picking and placing position and the constant temperature culturing position, a sucking position is arranged between the constant temperature culturing position and the selecting position, an inoculating position is arranged between the selecting position and the cleaning and sterilizing position, a rotatable tray 2 is arranged on the base 1, a placing opening 3 for placing a vessel 16 is arranged on the circumference of the tray 2, an L-shaped top plate 6 is fixedly connected with the upper side of the base 1, a rotatable turntable 4 is arranged below the L-shaped top plate 6, a lower driving shaft 21 is rotatably connected on the base 1, the upper end of the lower driving shaft 21 is fixedly connected with the center of the tray 2, the top of the L-shaped top plate 6 is rotatably connected with an upper driving shaft 20, the lower end of the upper driving shaft 20 is fixedly connected with the center of the turntable 4, a first motor can drive the upper driving shaft 20 and the lower driving shaft 21 to rotate positively and negatively, the tray 2 and the turntable 4 rotate, and the tray 16 is only required to be placed on the tray 2 automatically according to time to the placing opening 3 for placing a sample or the vessel 16 for placing the medium, mixing, the bacteria can be mixed, the bacteria can be heated, the bacteria can be automatically heated, the cultured and sterilized, the bacteria can be heated, the bacteria can be cleaned and sterilized, and cultured and heated.

The top of carousel 4 corresponds mixed position, absorb position and inoculation position department all fixed mounting have first electric telescopic handle 18, three are corresponding mixed position, absorb position and inoculation position department first electric telescopic handle 18's flexible end is installed respectively and is mixed the subassembly, absorb subassembly and location inoculation subassembly, carousel 4 circumference is equipped with the image recognition equipment 8 that corresponds arc mouth 5 is installed at the top of a plurality of arc mouths 5,L type roof 6, image recognition equipment 8 includes the camera and the treater, the camera is installed at the top of L type roof 6, treater and controller 7 electric connection, shoot the bismuth sulfite agar culture dish surface colony through arc mouth 5, because the form of salmonella colony in the bismuth sulfite agar culture dish is circular, the surface is smooth, the edge is neat, the colour has black metal luster, can link to network and download the data of the form colour of salmonella and various fungus crowd, the picture is input in the database, the camera can be directly perceived and the focus is shot out many pictures, the picture is uploaded to the treater, image processing and image analysis module are included in the treater, carry out noise removal by image processing module, the conventional filter, the picture is carried out, the position is carried out to the picture is accurate to the picture, the position is picked up to the position by the picture analysis module, carry out the position accuracy is compared with the picture, the position is picked out the position accuracy and is good at the position is compared with the position is located the module, and is located the position and is carried out the position accurately and is located to the position and is located.

In order to further improve the accuracy of identification, the image identification device can adopt a microorganism microscopic image identification technology, and microscopic pictures can be shot through an electron microscope, so that the microorganism shape can be directly identified accurately.

Further, the rotating shaft 24 is rotatably connected to the base 1 at a constant temperature culturing position, the upper end of the rotating shaft 24 is fixedly connected with the material tray 14 located between the turntable 4 and the tray 2, the arc-shaped heat preservation cover 15 covering the left half material tray 14 is further installed at the constant temperature culturing position, the arc-shaped heating plate 25 is installed on the inner wall of the arc-shaped heat preservation cover 15, the lower driving shaft 21, the upper driving shaft 20 and the rotating shaft 24 are driven by corresponding first motors, the feeding and discharging mechanism is further installed at the constant temperature culturing position, the feeding and discharging mechanism comprises a plurality of annular electromagnets 26 fixedly installed at the bottom of the material tray 14 in the circumferential direction, an iron ring 17 matched with the annular electromagnets 26 is sleeved at the rear of the material tray 16, a second electric telescopic rod 23 is fixedly connected to the base 1 at the constant temperature culturing position, the telescopic end of the second electric telescopic rod 23 is fixedly connected with a supporting plate 22 capable of jacking the material tray 16, and a homogenized sample is transferred to the constant temperature culturing position or can be transferred to the constant temperature culturing position for constant temperature culturing during selective separation culturing and inoculation culturing.

Specifically, the mixing assembly and the positioning inoculation assembly both comprise a second motor 27 fixedly installed at the telescopic end corresponding to the first electric telescopic rod 18, the mixing assembly further comprises a stirring rod 30 fixedly installed at the output end corresponding to the second motor 27, stirring blades are installed at the bottom of the stirring rod 30, when the sample vessel 16 rotates to a mixing position, the first electric telescopic rod 18 at the corresponding position is started to drive the stirring rod 30 to descend into the vessel 16, the corresponding second motor 27 is started, and the stirring blades can be driven to rotate to complete the homogeneous mixing process.

Specifically, the location inoculation subassembly includes fixed mounting at the electronic slide rail 28 that corresponds the second motor 27 output, the bottom slidable mounting of electronic slide rail 28 has inoculation ring 29, receive the signal of controller 7, start the first electric telescopic handle 18 of corresponding position, drive inoculation ring 29 decline, rethread starts corresponding second motor 27 and electronic slide rail 28 and rotates inoculation ring 29 circumference and radial movement, accurate removal is to the position of salmonella bacterial colony, scrape the bacterial colony of getting the salmonella through inoculation ring 29, later rotate new culture dish to the location inoculation position and carry out the marking inoculation.

Specifically, the sucking component comprises a liquid suction pipe 31 fixedly connected to the telescopic end of the corresponding first electric telescopic rod 18, a piston 33 is elastically connected to the liquid suction pipe 31 through a spring 32, and a liquid suction head 34 is arranged at the bottom of the liquid suction pipe 31, so that automatic sucking and dropping processes are realized.

Further, the top lateral wall fixedly connected with annular plate 10 of L type roof 6, be located on the annular plate 10 and mix the position, absorb the position, location inoculation position and wash the position department of disinfecting and all install electrically conductive seat 9, the top of three first electric telescopic handle 18 all fixed mounting have with electrically conductive seat 9 complex electrically conductive head 19, the controller 7 is installed at the top of L type roof 6 to second motor 27, electric slide rail 28 and spring 32 all with the electrically conductive head 19 electric connection of corresponding position, controller 7 and electrically conductive seat 9 electric connection everywhere, electrically conductive head 19 and electrically conductive seat 9 are last all to include many solitary electrically conductive ends, solitary electrically conductive end respectively electric connection corresponds first electric telescopic handle 18, second motor 27, spring 32 and electric slide rail 28, realize solitary control.

In addition, install the washing case 11 in washing the position department of disinfecting, the both sides of washing case 11 all are equipped with the opening 35 that supplies carousel 4 and tray 2 to pass, the washing tank 39 that is located between tray 2 and carousel 4 is fixedly connected with in the washing case 11, the water pump 41 that can pump into the sterilizing fluid is installed to the bottom of washing case 11, be connected with drain pipe 40 between the water outlet of water pump 41 and washing tank 39, still install drying tube 43 in the washing case 11, the top of drying tube 43 is equipped with a plurality of apopores, the top of washing case 11 is equipped with household utensils slot 13, household utensils slot 13 internal rotation is connected with wash pipe 42, the lateral wall and the top of wash pipe 42 all are equipped with brush roller 12, the interior top of washing case 11 is installed third motor 36, the output of third motor 36 meshes through a pair of gear 37 with wash pipe 42 between, the lateral wall of drain pipe 40 still is connected with branch pipe 38, the end and the wash pipe 42 lower extreme butt joint through rotary joint, install the valve in branch pipe 38, can realize to inoculation ring 29, the cleaning of mixing components and the process of drying.

The sample is loaded and the bacteria increasing liquid is placed in the vessel 16, then the vessel 16 is placed in the placing port 3 of the tray 2 at the taking and placing position, the tray 2 is driven to rotate by the driving of the first motor and the lower driving shaft 21, when the sample vessel 16 is rotated to the mixing position, the first electric telescopic rod 18 at the corresponding position is started to drive the stirring rod 30 to descend into the vessel 16, the corresponding second motor 27 is started, and the stirring blade can be driven to rotate to complete the homogeneous mixing process.

The homogenized sample is transferred to a constant temperature culturing position, and then the second electric telescopic rod 23 is started to drive the supporting plate 22 to move upwards, so that the vessel 16 to be cultured is supported to ascend, the iron ring 17 at the mouth of the vessel is magnetically attracted with the annular electromagnet 26 at the corresponding position at the bottom of the material tray 14, the material tray 14 is driven to rotate by the rotating shaft 24, the vessel 16 is transferred to the arc-shaped heat preservation cover 15, the heat preservation culturing process is realized by the inner arc-shaped heating plate 25, and a plurality of vessels 16 can be accommodated in the arc-shaped heat preservation cover 15 at one time.

The vessel 16 after bacteria increase is rotated to the sucking position, only the first electric telescopic rod 18 at the corresponding position is started to drive the liquid suction pipe 31 to be inserted into the vessel 16, the controller 7 controls the electrifying of the conductive seat 9 at the position, the spring 32 is electrified to shrink to drive the piston 33 to move upwards, then bacteria liquid is sucked into the liquid suction pipe 31 through the liquid suction head 34, then the bismuth sulfite agar culture dish is rotated to the sucking position, the spring 32 is powered off, the piston 33 moves downwards, the bacteria liquid is dripped onto the culture dish, and the automatic sucking and dripping processes are realized.

The drip-fed bismuth sulfite agar culture dish is transferred to a constant temperature culture position, the constant temperature culture is carried out in an arc-shaped heat preservation cover 15, the culture is transferred to a selection position, an image recognition device 8 is adopted, bacterial colonies on the surface of the bismuth sulfite agar culture dish are shot through an arc-shaped opening 5, the overall form of the bacterial colonies of salmonella in the bismuth sulfite agar culture dish is circular, smooth in surface and neat in edge, the color has black metallic luster, the data of the morphological colors of salmonella and various bacterial groups can be downloaded in a networking manner, the data are recorded into a database, a plurality of pictures can be intuitively and focused and shot by a camera, the pictures are uploaded to a processor, the processor comprises an image processing module and an image analysis module, the image processing module performs conventional filtering, denoising, area division and enhancement preprocessing on the images, the image analysis module extracts bacterial colony characteristics of each area in the pictures, the color is recognized, the salmonella bacterial colony is compared with salmonella parameter in the database, the salmonella bacterial colony is accurately selected, the processor is electrically connected with a positioning module, picture information of the recognized salmonella is transmitted to the positioning module, a digital coordinate system is established by the positioning module, the positioning module is used for accurately controlling the positioning of the salmonella bacterial colonies, and the positioning module is inoculated with a positioning assembly 7, and the positioning assembly is used for controlling the positioning assembly.

In order to further improve the accuracy of identification, the image identification device can adopt a microorganism microscopic image identification technology, a microscopic picture can be shot through an electron microscope, the shape of the microorganism is directly identified to accurately identify, and the characteristic value of the microorganism image is extracted as follows: 5 texture features (contrast, second moment, correlation of pixels to gray scale, inverse moment, entropy) based on gray co-occurrence matrix; the geometric features are 3 (circumference, area and circularity), and the salmonella is finally and rapidly identified by processing and analyzing the image by utilizing the existing mature identification technology.

The signal of the controller 7 is received, the first electric telescopic rod 18 at the corresponding position is started to drive the inoculating loop 29 to descend, the inoculating loop 29 is started to rotate circumferentially and move radially through the corresponding second motor 27 and the electric sliding rail 28, the inoculating loop 29 is accurately moved to the position of salmonella colonies, the salmonella colonies are scraped through the inoculating loop 29, then a new culture dish is transferred to a positioning inoculating position, and the inoculating dish is transferred to a constant-temperature culturing position for constant-temperature culturing after inoculation, so that the purification culturing process is realized.

The inoculating loop 29, the liquid suction pipe 31 and the mixing component are required to be cleaned and sterilized after being used, the three components are only required to be rotated into the cleaning tank 11 and positioned above the cleaning tank 39, the first electric telescopic rod 18 is controlled to be started again, the inoculating loop 29, the liquid suction pipe 31 and the mixing component can be respectively inserted into sterilizing liquid in the cleaning tank 39 to complete the cleaning and sterilizing process, then hot air blown out through the drying pipe 43 realizes the quick drying process, the vessel 16 is placed in the vessel slot 13 in a detachable mode, the third motor 36 is started, the flushing pipe 42 and the brush roller 12 are driven to rotate through the pair of gears 37 to complete the brushing process of the inner wall of the vessel 16, meanwhile, the valve is opened, the sterilizing liquid is fed into the flushing pipe 42 through the branch pipe 38 by the water pump 41, and then the sterilizing and cleaning process is realized through ejection.

The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims

1. The utility model provides a salmonella pathogen separation purification equipment, includes base (1), its characterized in that, the front side of base (1) is for getting and puts the position, and the left side is for the constant temperature cultivation position, and the rear side is for choosing the position that disinfects, get and put and be mixed between the constant temperature cultivation position and the position is cultivateed to the constant temperature, be the position of absorbing between the position is chosen to constant temperature cultivation position, choose between position and the position that disinfects to wash and be inoculation position, install rotatable tray (2) on base (1), be equipped with on tray (2) circumference and be used for placing mouth (3) of household utensils (16), rotatable carousel (4) are installed to the top of L type roof (6), the top of carousel (4) corresponds mixed position, absorbs position and inoculation position department and all fixed mounting have first electric telescopic handle (18), and three correspond mixed subassembly, absorb position department and first electric telescopic handle (18) the end is installed respectively and is connected with top (21) of carousel (1) and is equipped with rotatory top (4) of type roof (1), the top (2) is connected with top (21) and is equipped with arc-shaped top (1) and is equipped with the arc-shaped and is connected with top (21) of arc-shaped roof (5), the top of L type roof (6) rotates and is connected with drive shaft (20), the lower extreme and carousel (4) center fixed connection of last drive shaft (20), it is connected with pivot (24) to cultivate the department rotation in constant temperature on base (1), the upper end fixedly connected with of pivot (24) is located charging tray (14) between carousel (4) and tray (2), the arc heat preservation cover (15) of half charging tray (14) in cover are still installed to constant temperature cultivation department, arc hot plate (25) are installed to the inner wall of arc heat preservation cover (15), first motor drive that corresponds is located in lower drive shaft (21), last drive shaft (20) and pivot (24), the constant temperature is cultivateed department and is still installed feed mechanism, last feed mechanism includes a plurality of annular electromagnets (26) of circumference fixed mounting in charging tray (14) bottom, the cover is equipped with iron ring (17) with annular electromagnets (26) complex behind the ware of ware (16), the ware that is located the constant temperature cultivation department on base (1) and is located electric motor fixed connection second telescopic link (23), telescopic link (22) with flexible layer board (23).

2. The salmonella pathogen separation and purification apparatus of claim 1, wherein the mixing assembly and the positioning inoculation assembly each comprise a second motor (27) fixedly mounted at a telescoping end corresponding to the first motor telescoping rod (18), the mixing assembly further comprises a stirring rod (30) fixedly mounted at an output end corresponding to the second motor (27), and stirring blades are mounted at the bottom of the stirring rod (30).

3. The salmonella pathogen separation and purification apparatus of claim 2, wherein the positioning inoculation assembly comprises an electric slide rail (28) fixedly mounted at the output end of the corresponding second motor (27), and an inoculation ring (29) is slidably mounted at the bottom of the electric slide rail (28).

4. A salmonella pathogen separation and purification apparatus according to claim 3, wherein the suction assembly comprises a pipette (31) fixedly connected to the telescopic end of the corresponding first electric telescopic rod (18), a piston (33) is elastically connected to the pipette (31) through a spring (32), and a pipette head (34) is provided at the bottom of the pipette (31).

5. The salmonella pathogen separation and purification device of claim 4, wherein the top side wall of the L-shaped top plate (6) is fixedly connected with an annular plate (10), conductive seats (9) are respectively arranged on the annular plate (10) at a mixing position, a sucking position, a positioning inoculation position and a cleaning sterilization position, conductive heads (19) matched with the conductive seats (9) are respectively and fixedly arranged on the tops of the three first electric telescopic rods (18), and the second motor (27), the electric sliding rail (28) and the springs (32) are respectively and electrically connected with the conductive heads (19) at corresponding positions.

6. The salmonella pathogen separation and purification apparatus of claim 5, wherein the controller (7) is mounted on top of the L-shaped top plate (6), the image recognition apparatus (8) comprises a camera and a processor, the camera is mounted on top of the L-shaped top plate (6), the processor is electrically connected with the controller (7), and the controller (7) is electrically connected with the conductive seats (9) around.

7. The salmonella pathogen separation and purification apparatus of claim 6, wherein the cleaning and sterilizing place is provided with a cleaning tank (11), two sides of the cleaning tank (11) are provided with openings (35) for passing through the turntable (4) and the tray (2), a cleaning tank (39) between the tray (2) and the turntable (4) is fixedly connected in the cleaning tank (11), a water pump (41) capable of pumping sterilizing liquid is installed at the bottom of the cleaning tank (11), a liquid outlet pipe (40) is connected between the water outlet end of the water pump (41) and the cleaning tank (39), a drying pipe (43) is also installed in the cleaning tank (11), the top of the drying pipe (43) is provided with a plurality of air outlet holes, the top of the cleaning tank (11) is provided with a vessel slot (13), a flushing pipe (42) is rotationally connected in the vessel slot (13), the side wall and the top of the flushing pipe (42) are provided with a brush roller (12), a third motor (11) is provided with a liquid outlet pipe (40) connected with a third side wall (36) through a rotary joint (38) between the third motor (36) and the third side wall (38), a valve is arranged in the branch pipe (38).