CN115725385A - Salmonella pathogen separation and purification equipment - Google Patents
Salmonella pathogen separation and purification equipment Download PDFInfo
- Publication number
- CN115725385A CN115725385A CN202211546772.6A CN202211546772A CN115725385A CN 115725385 A CN115725385 A CN 115725385A CN 202211546772 A CN202211546772 A CN 202211546772A CN 115725385 A CN115725385 A CN 115725385A
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- box body
- wall
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- mixing tank
- scraping
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- 238000000746 purification Methods 0.000 title claims abstract description 28
- 241000607142 Salmonella Species 0.000 title claims abstract description 24
- 238000000926 separation method Methods 0.000 title claims abstract description 24
- 244000052769 pathogen Species 0.000 title claims abstract description 17
- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 17
- 238000007790 scraping Methods 0.000 claims abstract description 34
- 241001465754 Metazoa Species 0.000 claims abstract description 14
- 210000001519 tissue Anatomy 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 9
- 239000007853 buffer solution Substances 0.000 claims abstract description 8
- 210000004400 mucous membrane Anatomy 0.000 claims abstract description 5
- 210000004347 intestinal mucosa Anatomy 0.000 claims description 12
- 230000006835 compression Effects 0.000 claims description 8
- 238000007906 compression Methods 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 210000001072 colon Anatomy 0.000 description 21
- 229920001817 Agar Polymers 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 241000588921 Enterobacteriaceae Species 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses salmonella pathogen separation and purification equipment, which comprises a box body, a supporting mechanism, a scraping mechanism, a mixing tank, a culture tank and an absorbing mechanism, wherein the supporting mechanism is arranged on a supporting platform and used for propping open animal tissues, the scraping mechanism is used for scraping mucous membranes of the animal tissues, the mixing tank is used for bearing PBS buffer solution, the culture tank is used for bearing TTB enriched solution, the absorbing mechanism is used for absorbing mixed solution in the mixing tank, a first driving mechanism for driving the scraping mechanism to move from the supporting mechanism to the mixing tank is arranged on the inner wall of the box body, and a second driving mechanism for driving the absorbing mechanism to move from the mixing tank to the culture tank is also arranged on the inner wall of the box body; when carrying out the separation and purification experiment, the animal tissue cover with the intercepting struts on supporting mechanism, scrapes through scraping the mechanism and gets partly with the mucous membrane of animal tissue, and first actuating mechanism drive is scraped and is got the mechanism and remove to the mixing tank in, and the mixed liquid that second actuating mechanism drive absorption mechanism absorbed in the mixing tank removes cultivates in the culture tank, realizes the autosegregation purification of salmonella.
Description
Technical Field
The invention relates to the technical field of soil microorganisms, in particular to salmonella pathogen separation and purification equipment.
Background
Salmonella is a member of the genus salmonella of the family enterobacteriaceae, a class of gram-negative enterobacteriaceae that are conditionally intracellular parasitic. In the drug resistance detection of salmonella, colon contents of sick pigs are taken to separate and purify bacteria.
The separation and purification process is as follows: directly shearing a section of animal tissue of about 10cm, such as colon, putting the animal tissue into a sterile plastic bag and bringing the animal tissue back to a laboratory, scraping the mucous membrane of the animal tissue, such as intestinal mucous membrane, in 2h by using a sterile surgical blade, putting the animal tissue into autoclaved PBS buffer solution and uniformly mixing, sucking 10 microliter of the mixed solution by using a sterile suction head and inoculating the mixed solution into 4ml LTTB enriched solution, culturing for 12-16 h at 37 ℃ at 200r/min, then streaking and inoculating the 1-ring culture solution by using an inoculating loop to a Mackanka agar culture medium, and inoculating the suspicious single colony to another Mackanka agar culture medium for purification. The existing operation process is carried out manually, and the manual intervention can cause sample pollution and influence the precision of an experimental result.
Disclosure of Invention
The invention aims to solve the problems and provides salmonella pathogen separation and purification equipment which can realize automatic separation and purification of salmonella, reduce artificial participation and prevent sample pollution.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a salmonella pathogen separation and purification device comprises a box body, a supporting mechanism, a scraping mechanism, a mixing tank, a culture tank and an absorbing mechanism, wherein the supporting mechanism is arranged on a supporting platform and used for propping open animal tissues, the scraping mechanism is used for scraping intestinal mucosa in the animal tissues, the mixing tank is used for bearing PBS buffer solution, the culture tank is used for bearing TTB enriched solution, and the absorbing mechanism is used for absorbing mixed solution in the mixing tank;
the supporting mechanism comprises first sleeves which are symmetrically arranged and fixed at the bottom of the box body, gaps are arranged among the first sleeves which are symmetrically arranged, and openings are formed in the first sleeves;
the scraping mechanism comprises a scraper matched with the opening and used for scraping intestinal mucosa and a first motor driving the scraper to rotate;
the first driving mechanism comprises a first moving plate which is arranged on the inner wall of the box body and moves left and right, and a first lifting frame which is arranged on the first moving plate and moves on the first moving plate, wherein a first guide rail for guiding the moving track of the first lifting frame is arranged on the inner wall of the box body;
the suction mechanism comprises an aseptic suction head, the second driving mechanism comprises a second movable plate and a second lifting frame, the second movable plate is arranged on the inner wall of the box body and moves left and right, the second lifting frame is arranged on the second movable plate and moves on the second movable plate, the aseptic suction head is fixed on the second lifting frame, a second guide rail guiding the moving track of the second lifting frame is arranged on the inner wall of the box body, and a third driving mechanism driving a piston rod of the aseptic suction head is arranged on the second lifting frame.
Further, a second sleeve is arranged at two ends of the output shaft of the first motor, the scraper is located in the second sleeve, a compression spring is arranged between one end of the inner side of the scraper and the inner wall of the second sleeve, and a positioning mechanism is arranged between the scraper and the second sleeve.
Further, positioning mechanism is including setting up the connecting rod on the second sleeve, rotate between inboard one end of connecting rod and the second sleeve and be connected, connecting rod outside one end is equipped with first locating head, the scraper upper end be equipped with first locating head complex second locating head, be equipped with the horizontal pole on the connecting rod.
Furthermore, a first guide rod is arranged on the inner wall of the box body, a guide sleeve matched with the first guide rod is arranged at the rear end of the first movable plate, a first lead screw in threaded connection with the first movable plate is further arranged on the inner wall of the box body, and a second motor for driving the first lead screw to rotate is arranged on the outer side of the box body.
Further, the first guide rail includes a horizontal portion in the middle and arc portions at both sides of the horizontal portion.
Furthermore, a first guide wheel matched with the first guide rail is arranged at the rear end of the first lifting frame.
Furthermore, a second guide rod is arranged on the inner wall of the box body, a guide sleeve matched with the first guide rod is arranged at the rear end of the second moving plate, a second lead screw in threaded connection with the second moving plate is further arranged on the inner wall of the box body, and a third motor for driving the second lead screw to rotate is arranged on the outer side of the box body.
Further, the second guide rail includes a horizontal portion in the middle and arc portions at both sides of the horizontal portion.
Furthermore, a first guide wheel matched with the second guide rail is arranged at the rear end of the second lifting frame.
Furthermore, the third driving mechanism comprises a fourth motor installed on the second lifting frame, a cylindrical head is arranged on an output shaft of the fourth motor, a spiral guide groove is formed in the outer cylindrical surface of the cylindrical head, a third guide wheel is arranged at the upper end of the piston rod of the sterile suction head, and the second guide rail is matched with the guide groove.
The invention has the beneficial effects that:
1. the colon scraping and taking device comprises a box body, a supporting mechanism, a scraping and taking mechanism, a mixing tank, a culture tank and an absorbing mechanism, wherein the supporting mechanism is arranged on a supporting platform and used for stretching the colon, the scraping and taking mechanism is used for scraping intestinal mucosa in the colon, the mixing tank is used for bearing PBS buffer solution, the culture tank is used for bearing TTB enriched solution, the absorbing mechanism is used for absorbing mixed solution in the mixing tank, a first driving mechanism for driving the scraping and taking mechanism to move from the supporting mechanism to the mixing tank is arranged on the inner wall of the box body, and a second driving mechanism for driving the absorbing mechanism to move from the mixing tank to the culture tank is also arranged on the inner wall of the box body; when a separation and purification experiment is carried out, the intercepted colon sleeve is stretched on a supporting mechanism, a part of intestinal mucosa on the inner wall of the colon is scraped by a scraping mechanism, the scraping mechanism is moved into a mixing tank under the action of a first driving mechanism, the scraped intestinal mucosa is mixed with PBS buffer solution in the mixing tank, then a second driving mechanism drives an absorbing mechanism to absorb mixed solution in the mixing tank and drives the absorbing mechanism to move into a culture tank for culture, after the culture is finished, inoculating 1-ring culture solution from an inoculating ring fishing ring is streaked and inoculated into a Mackanka agar culture medium, and a suspected single bacterial colony is inoculated into another Mackanka agar culture medium for purification. The automatic separation and purification of salmonella are realized, the manual participation is reduced, the sample pollution is prevented, and the experiment precision is improved.
2. The positioning mechanism comprises a connecting rod arranged on a second sleeve, one end of the inner side of the connecting rod is rotatably connected with the second sleeve, a first positioning head is arranged at one end of the outer side of the connecting rod, a second positioning head matched with the first positioning head is arranged at the upper end of the scraper, and a cross rod is arranged on the connecting rod. Before the scraper enters the colon, the scraper overcomes the elastic force of the compression spring and is fixed in the second sleeve through the connecting rod, so that the cutter can enter the colon conveniently. When the scraper completely penetrates into the colon, the cross rod is in contact with the upper end of the second sleeve, the connecting rod rotates, the first positioning head is separated from the second positioning head, and the compression spring pushes out the scraper to be in contact with the inner wall of the colon.
3. The first guide rail comprises a horizontal part in the middle and arc parts positioned on two sides of the horizontal part, and the rear end of the first lifting frame is provided with a first guide wheel matched with the first guide rail. Through the moving track of the first lifting frame guided by the first guide rail, one drive can be realized, the first lifting frame is driven to lift without independent drive, and the cost is reduced.
Drawings
In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a first schematic structural view of a scraping mechanism according to the present invention;
FIG. 4 is a second schematic structural view of the scraping mechanism of the present invention;
FIG. 5 is a third schematic view of the scraping mechanism of the present invention;
FIG. 6 is a cross-sectional view of the doctor blade of the present invention;
FIG. 7 is a schematic view of the sucking mechanism of the present invention;
fig. 8 is a front view of the suction mechanism of the present invention.
In the figure: the device comprises a box body 1, a mixing tank 2, a culture tank 3, a first sleeve 4, a gap 5, an opening 6, a scraper 7, a first motor 8, a first moving plate 9, a first lifting frame 10, a first guide rail 11, a sterile suction head 12, a second moving plate 13, a second lifting frame 14, a second guide rail 15, a second sleeve 16, a compression spring 17, a connecting rod 18, a first positioning head 19, a second positioning head 20, a cross rod 21, a first guide rod 22, a first lead screw 23, a second motor 24, a first guide wheel 25, a second guide rod 26, a second lead screw 27, a third motor 28, a fourth motor 29, a cylindrical head 30, a guide groove 31 and a third guide wheel 32.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiment of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 and fig. 2, a salmonella pathogen separation and purification device comprises a box body 1, a supporting mechanism arranged on the supporting platform 1 for supporting the colon, a scraping mechanism for scraping off intestinal mucosa in the colon, a mixing tank 2 for carrying PBS buffer solution, an incubation tank 3 for carrying TTB enriched solution, and an absorbing mechanism for absorbing mixed solution in the mixing tank 2, wherein a first driving mechanism for driving the scraping mechanism to move from the supporting mechanism to the mixing tank 2 is arranged on the inner wall of the box body 1, and a second driving mechanism for driving the absorbing mechanism to move from the mixing tank 2 to the incubation tank 3 is also arranged on the inner wall of the box body 1;
when a separation and purification experiment is carried out, the intercepted colon sleeve is stretched on a supporting mechanism, a part of intestinal mucosa on the inner wall of the colon is scraped through a scraping mechanism, the scraping mechanism is moved into a mixing tank 2 under the action of a first driving mechanism, the scraped intestinal mucosa is mixed with PBS buffer solution in the mixing tank 2, then a second driving mechanism drives the sucking mechanism to suck mixed solution in the mixing tank 2 and drives the sucking mechanism to move into a culture tank 3 for culture, after the culture is finished, 1-ring culture solution is obtained through inoculating fishing, streaked and inoculated in a Mackanka agar culture medium, and suspected single colony is inoculated in another Mackanka agar culture medium for purification. The automatic separation and purification of salmonella are realized, the manual participation is reduced, the sample pollution is prevented, and the experiment precision is improved.
The animal tissue in this example is illustrated by the colon.
As shown in fig. 3 and 4, the supporting mechanism includes first sleeves 4 symmetrically fixed at the bottom of the box body 1, gaps 5 are provided between the first sleeves 4 symmetrically arranged, and openings 6 are provided on the first sleeves 4; the colon is propped open by the first sleeve 4, so that the intestinal mucosa is conveniently scraped by a scraper.
As shown in fig. 3 and 4, the scraping mechanism comprises a scraper 7 which is matched with the opening 6 for scraping the intestinal mucosa and a first motor 8 which drives the scraper 7 to rotate; the scraper 7 penetrates into the opening positions of the two first sleeves 4 through the gap 5 to prevent interference between the sleeves and the scraper, and then the first motor 8 drives the scraper 7 to rotate, so that the intestinal mucosa is scraped.
As shown in fig. 1, the first driving mechanism includes a first moving plate 9 disposed on the inner wall of the box body 1 and moving left and right, and a first lifting frame 10 disposed on the first moving plate 9 and moving on the first moving plate 9, the first lifting frame 10 is connected with the first moving plate 9 in a sliding manner through a guide rail and slider pair, and a first guide rail 11 guiding the moving track of the first lifting frame 10 is disposed on the inner wall of the box body 1;
as shown in fig. 2 and 7, the suction mechanism includes a sterile suction head 12, the second driving mechanism includes a second moving plate 13 disposed on the inner wall of the box 1 and moving left and right, and a second lifting frame 14 disposed on the second moving plate 13 and moving on the second moving plate 13, the second lifting frame 14 is connected with the second moving plate 13 in a sliding manner through a guide rail and slider pair, the sterile suction head 12 is fixed on the second lifting frame 14, a second guide rail 15 guiding the moving track of the second lifting frame 14 is disposed on the inner wall of the box 1, and a third driving mechanism for driving a piston rod of the sterile suction head 12 is disposed on the second lifting frame 14.
As shown in fig. 6, two ends of the output shaft of the first motor 8 are provided with a second sleeve 16, the scraper 7 is located in the second sleeve 16, a compression spring 17 is arranged between one end of the inner side of the scraper 7 and the inner wall of the second sleeve 16, and a positioning mechanism is arranged between the scraper 7 and the second sleeve 16.
As shown in fig. 6, the positioning mechanism includes a connecting rod 18 disposed on the second sleeve 16, one end of the inner side of the connecting rod 18 is rotatably connected to the second sleeve 16, a first positioning head 19 is disposed at one end of the outer side of the connecting rod 18, a second positioning head 20 matched with the first positioning head 19 is disposed at the upper end of the scraper 7, and a cross rod 21 is disposed on the connecting rod 18. Before the spatula 7 reaches the colon, the spatula 7 overcomes the elastic force of the compression spring 17 and the spatula 7 is fixed in the second sleeve 16 by the connecting rod 18, so that the knife can be conveniently inserted into the colon. When the scraper 7 completely penetrates into the colon, the cross rod 21 is contacted with the upper end of the second sleeve 16, the connecting rod 18 rotates, the first positioning head 19 is separated from the second positioning head 20, and the compression spring 17 pushes the scraper 7 out to be contacted with the inner wall of the colon.
As shown in fig. 1 and 2, a first guide rod 22 is disposed on an inner wall of the box body 1, a guide sleeve matched with the first guide rod 22 is disposed at a rear end of the first moving plate 9, a first lead screw 23 in threaded connection with the first moving plate 9 is further disposed on the inner wall of the box body 1, two ends of the first lead screw 23 are rotatably connected with the inner wall of the box body 1, a nut in threaded connection with the first lead screw 23 is disposed at the rear end of the first moving plate 9, a second motor 24 for driving the first lead screw 23 to rotate is disposed on an outer side of the box body 1, and a lead screw nut driving manner is the prior art and is not described herein in any more detail.
As shown in fig. 2 and 5, the first guide rail 11 includes a horizontal portion in the middle and arc portions at both sides of the horizontal portion, the left side arc portion having a bottom corresponding to the support mechanism and the right side arc portion having a bottom corresponding to the mixing tub 2. The rear end of the first lifting frame 10 is provided with a first guide wheel 25 matched with the first guide rail 11. Through the moving track of the first guide rail guiding first lifting frame, one driving can be realized, independent driving is not needed, the first lifting frame is driven to lift, and the cost is reduced.
As shown in fig. 2, a second guide rod 26 is arranged on the inner wall of the box body 1, a guide sleeve matched with the first guide rod 22 is arranged at the rear end of the second moving plate 13, a second lead screw 27 in threaded connection with the second moving plate 13 is further arranged on the inner wall of the box body 1, two ends of the second lead screw 27 are rotatably connected with the inner wall of the box body 1, a nut in threaded connection with the second lead screw 27 is arranged at the rear end of the second moving plate 13, a third motor 28 for driving the second lead screw 27 to rotate is arranged on the outer side of the box body 1, and the lead screw nut driving mode is the prior art and is not described in detail herein.
As shown in FIG. 2, the second guide rail 15 includes a horizontal portion in the middle and arc-shaped portions at both sides of the horizontal portion, the left side arc-shaped portion having a bottom corresponding to the mixing tank 2 and the right side arc-shaped portion having a bottom corresponding to the culture tank 3. And a first guide wheel matched with the second guide rail 15 is arranged at the rear end of the second lifting frame 14. Through the moving track of second crane of second guide rail 15 direction, a drive can, need not independent drive second crane lift, reduce cost.
As shown in fig. 8, the third driving mechanism includes a fourth motor 29 installed on the second lifting frame 14, a cylinder head 30 is installed on an output shaft of the fourth motor 29, a spiral guide groove 31 is installed on an outer cylindrical surface of the cylinder head 30, a third guide wheel 32 is installed at the upper end of a piston rod of the sterile suction head 12, the second guide rail 15 is matched with the guide groove 31, the cylinder head 30 is driven to rotate by the motor, and the lifting of the piston rod of the sterile suction head 12 is realized under the action of the third guide wheel 32 and the guide groove 31.
In the description of the present invention, it should be noted that the terms "left", "right", "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; they may be mechanically or electrically connected, directly or indirectly through intervening media, or may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Claims (10)
1. The salmonella pathogen separation and purification equipment is characterized by comprising a box body (1), a supporting mechanism, a scraping mechanism, a mixing tank (2), a culture tank (3) and an absorbing mechanism, wherein the supporting mechanism is arranged on a supporting platform (1) and used for propping up animal tissues, the scraping mechanism is used for scraping mucous membranes of the animal tissues, the mixing tank (2) is used for bearing PBS buffer solution, the culture tank (3) is used for bearing TTB enriched solution, the absorbing mechanism is used for absorbing the mixed solution in the mixing tank (2), a first driving mechanism for driving the scraping mechanism to move to the mixing tank (2) from the supporting mechanism is arranged on the inner wall of the box body (1), and a second driving mechanism for driving the absorbing mechanism to move to the culture tank (3) from the mixing tank (2) is also arranged on the inner wall of the box body (1);
the supporting mechanism comprises first sleeves (4) which are symmetrically arranged and fixed at the bottom of the box body (1), gaps (5) are arranged between the symmetrically arranged first sleeves (4), and openings (6) are arranged on the first sleeves (4);
the scraping mechanism comprises a scraper (7) matched with the opening (6) and used for scraping intestinal mucosa and a first motor (8) driving the scraper (7) to rotate;
the first driving mechanism comprises a first moving plate (9) which is arranged on the inner wall of the box body (1) and moves left and right, and a first lifting frame (10) which is arranged on the first moving plate (9) and moves on the first moving plate (9), wherein a first guide rail (11) for guiding the moving track of the first lifting frame (10) is arranged on the inner wall of the box body (1);
the suction means includes aseptic suction head (12), second actuating mechanism is including setting up second movable plate (13) and setting second crane (14) that move on second movable plate (13) and moving on second movable plate (13) about on box (1) inner wall, aseptic suction head (12) are fixed on second crane (14), be equipped with second guide rail (15) that direction second crane (14) removed the orbit on box (1) inner wall, be equipped with the third actuating mechanism of drive aseptic suction head (12) piston rod on second crane (14).
2. The salmonella pathogen separation and purification device according to claim 1, wherein a second sleeve (16) is arranged at two ends of the output shaft of the first motor (8), the scraper (7) is positioned in the second sleeve (16), a compression spring (17) is arranged between one end of the inner side of the scraper (7) and the inner wall of the second sleeve (16), and a positioning mechanism is arranged between the scraper (7) and the second sleeve (16).
3. The salmonella pathogen separation and purification device according to claim 2, wherein the positioning mechanism comprises a connecting rod (18) arranged on the second sleeve (16), one end of the inner side of the connecting rod (18) is rotatably connected with the second sleeve (16), one end of the outer side of the connecting rod (18) is provided with a first positioning head (19), the upper end of the scraper (7) is provided with a second positioning head (20) matched with the first positioning head (19), and the connecting rod (18) is provided with a cross rod (21).
4. The salmonella pathogen separation and purification equipment according to claim 1, wherein a first guide rod (22) is arranged on the inner wall of the box body (1), a guide sleeve matched with the first guide rod (22) is arranged at the rear end of the first moving plate (9), a first lead screw (23) in threaded connection with the first moving plate (9) is further arranged on the inner wall of the box body (1), and a second motor (24) for driving the first lead screw (23) to rotate is arranged outside the box body (1).
5. The salmonella pathogen separation and purification apparatus of claim 1, wherein the first guide rail (11) comprises a horizontal portion in the middle and arc-shaped portions at both sides of the horizontal portion.
6. The salmonella pathogen separation and purification apparatus according to claim 1, wherein the first crane (10) is provided at a rear end thereof with a first guide wheel (25) engaged with the first guide rail (11).
7. The salmonella pathogen separation and purification device according to claim 1, wherein a second guide rod (26) is arranged on the inner wall of the box body (1), a guide sleeve matched with the first guide rod (22) is arranged at the rear end of the second moving plate (13), a second lead screw (27) in threaded connection with the second moving plate (13) is further arranged on the inner wall of the box body (1), and a third motor (28) for driving the second lead screw (27) to rotate is arranged outside the box body (1).
8. The salmonella pathogen separation and purification apparatus according to claim 1, wherein the second guide rail (15) comprises a horizontal portion at the center and arc portions at both sides of the horizontal portion.
9. The salmonella pathogen separation and purification apparatus according to claim 1, wherein the second crane (14) is provided at the rear end thereof with a first guide wheel engaged with the second guide rail (15).
10. The salmonella pathogen separation and purification equipment according to claim 1, wherein the third driving mechanism comprises a fourth motor (29) installed on the second lifting frame (14), a cylindrical head (30) is arranged on an output shaft of the fourth motor (29), a spiral guide groove (31) is formed in an outer cylindrical surface of the cylindrical head (30), a third guide wheel (32) is arranged at the upper end of a piston rod of the sterile suction head (12), and the second guide rail (15) is matched with the guide groove (31).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211546772.6A CN115725385A (en) | 2022-12-05 | 2022-12-05 | Salmonella pathogen separation and purification equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211546772.6A CN115725385A (en) | 2022-12-05 | 2022-12-05 | Salmonella pathogen separation and purification equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115725385A true CN115725385A (en) | 2023-03-03 |
Family
ID=85300078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211546772.6A Withdrawn CN115725385A (en) | 2022-12-05 | 2022-12-05 | Salmonella pathogen separation and purification equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115725385A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116555000A (en) * | 2023-07-11 | 2023-08-08 | 青岛市动物疫病预防控制中心 | Salmonella pathogen separation and purification equipment |
-
2022
- 2022-12-05 CN CN202211546772.6A patent/CN115725385A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116555000A (en) * | 2023-07-11 | 2023-08-08 | 青岛市动物疫病预防控制中心 | Salmonella pathogen separation and purification equipment |
| CN116555000B (en) * | 2023-07-11 | 2023-09-12 | 青岛市动物疫病预防控制中心 | Salmonella pathogen separation and purification equipment |
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