CN112358960A - Intelligent automatic microorganism detection equipment and method thereof - Google Patents

Intelligent automatic microorganism detection equipment and method thereof Download PDF

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Publication number
CN112358960A
CN112358960A CN202011307255.4A CN202011307255A CN112358960A CN 112358960 A CN112358960 A CN 112358960A CN 202011307255 A CN202011307255 A CN 202011307255A CN 112358960 A CN112358960 A CN 112358960A
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China
Prior art keywords
light source
source base
specimen
light
sample carrier
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CN202011307255.4A
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Chinese (zh)
Inventor
苗静
齐宝宁
李娟�
徐守竹
周晶
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Shaanxi University of Chinese Medicine
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Shaanxi University of Chinese Medicine
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Priority to CN202011307255.4A priority Critical patent/CN112358960A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses intelligent automatic microorganism detection equipment and a method thereof.A light source reflecting film layer is arranged at the bottom in a specimen carrier light source base, a light strip type light source is arranged on the side edge in the specimen carrier light source base, a Fresnel lens layer is arranged at an opening in the middle of the specimen carrier light source base, a light-transmitting specimen carrier structure is arranged on the upper surface of the Fresnel lens layer, an electronic objective is connected with an electronic objective mounting rack through an electronic objective mounting threaded column, the lower end of the electronic objective mounting rack is detachably connected with the edge of the specimen carrier light source base, and a light-transmitting specimen cover plate is positioned on the upper surface of the light-transmitting specimen carrier structure. The light source and the specimen carrier are designed into a whole, so that the light source can fully penetrate through the specimen, the detection is clearer, the light source is not influenced by ambient light by carrying out closed detection on the objective lens and the specimen, the image presented in any occasion is not subjected to chromatic aberration caused by the influence of the ambient light, and the device is small and exquisite and is convenient to operate.

Description

Intelligent automatic microorganism detection equipment and method thereof
Technical Field
The invention relates to microorganism detection equipment, in particular to intelligent automatic microorganism detection equipment and a method thereof.
Background
The food microorganism inspection method is an essential important component part for food quality management, is a thorough 'prevention-oriented' guideline, can effectively prevent or reduce the occurrence of diseases of food, people and livestock, and guarantees the physical health of people. The food microbiological examination is one of the important indexes for measuring the food sanitation quality and is also one of the scientific bases for judging whether the detected food is eaten or not. Through food microbiological examination, the food processing environment and the food sanitation condition can be judged, the degree of the food polluted by bacteria can be correctly evaluated, and a scientific basis is provided for various sanitation management works. In the prior art, the detection of microorganisms is carried out through a microscope, the operation of the microscope is complex, the microscope is provided with a light source, the equipment is large, and the operation is complex, so that the improvement space exists.
Disclosure of Invention
The present invention is directed to solving the above problems and providing an intelligent and automatic microorganism detection apparatus and a method thereof.
The invention realizes the purpose through the following technical scheme:
the invention comprises a specimen carrier light source base, a light source reflecting film layer, a light belt type light source, a Fresnel lens layer, a light transmitting specimen carrier structure, a light transmitting specimen cover plate, an electronic objective lens mounting frame, an electronic objective lens and an electronic objective lens mounting threaded column, wherein the light source reflecting film layer is arranged at the bottom in the specimen carrier light source base, the light belt type light source is arranged on the side edge in the specimen carrier light source base in a circle, the Fresnel lens layer is arranged at an opening at the middle part of the specimen carrier light source base, the light transmitting specimen carrier structure is arranged on the upper surface of the Fresnel lens layer, the electronic objective lens is arranged at the lower end of the electronic objective lens mounting threaded column, the electronic objective lens mounting threaded column is in threaded connection with the middle part of the upper end of the electronic objective lens mounting frame, and the lower end of the electronic objective lens mounting frame, the light-transmitting specimen cover plate is positioned on the upper surface of the light-transmitting specimen carrier structure.
Further, the shape of printing opacity sample carrier structure is the lid shape, the upper end edge of sample carrier light source base is sunken to the inboard, printing opacity sample carrier structure lid closes the upper end of sample carrier light source base will the upper end of sample carrier light source base, the chenille lens layer the lamp area formula light source with light source reflection of light rete all wraps up in sample carrier light source base.
Preferably, the edge of sample carrier light source base is provided with waterproof sealing washer, waterproof sealing washer is located printing opacity sample carrier structure with between the sample carrier light source base.
As an improvement, the lower end edge of the electronic objective mounting rack is provided with a lower mounting rack magnetic attraction body, the edge of the specimen carrier light source base is provided with an upper mounting rack magnetic attraction body matched with the lower mounting rack magnetic attraction body, and the electronic objective mounting rack and the specimen carrier light source base are connected in a detachable mode through the lower mounting rack magnetic attraction body and the upper mounting rack magnetic attraction body.
As an improvement, the upper end edge of the specimen carrier light source base is provided with a lower cover plate magnetic attraction body, the upper end edge of the light-transmitting specimen cover plate is provided with an upper cover plate magnetic attraction body matched with the lower cover plate magnetic attraction body, and the upper cover plate magnetic attraction body is detachably and fixedly connected with the specimen carrier light source base through the lower cover plate magnetic attraction body and the upper cover plate magnetic attraction body.
The detection method of the intelligent automatic microorganism detection equipment comprises the following steps:
placing a microorganism specimen on the upper surface of a light-transmitting specimen carrier structure, and covering a light-transmitting specimen cover plate;
the light belt type light source is electrified to emit light, the light is reflected to the Fresnel lens layer through the light source reflecting film layer, and the Fresnel lens layer enables the light source to be uniformly scattered to penetrate through the light-transmitting specimen carrier structure and the light-transmitting specimen cover plate;
butting the electronic objective mounting frame with the specimen carrier light source base to enable the microbial specimen to be positioned below the electronic objective;
fourthly, the electronic objective lens is installed with the threaded column through spiral rotation, so that the electronic objective lens can move up and down, and the position of the electronic objective lens is adjusted;
the signal of the electronic objective lens is transmitted to a computer through a data line for detection and recording.
The invention has the beneficial effects that:
the invention is an intelligent automatic microorganism detection device and method, compared with the prior art, the invention has simple structure, convenient use, self-contained light source, and the light source and the specimen carrier are designed into a whole, so that the light source can fully penetrate the specimen, the detection is clearer, the light source is not influenced by the ambient light by carrying out closed detection on the objective and the specimen, the image presented in any occasion can not generate color difference due to the influence of the ambient light, and the device is small and exquisite, is convenient to operate, and has popularization and application values.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Detailed Description
The invention will be further described with reference to the accompanying drawings in which:
as shown in fig. 1: the specimen light source device comprises a specimen carrier light source base 1, a light source reflection film layer 2, a light belt type light source 3, a Fresnel lens layer 4, a light transmitting specimen carrier structure 5, a light transmitting specimen cover plate 6, an electronic objective lens mounting frame 7, an electronic objective lens 8 and an electronic objective lens mounting threaded column 9, wherein the light source reflection film layer 2 is arranged at the bottom in the specimen carrier light source base 1, the light belt type light source 3 is arranged on the side edge in the specimen carrier light source base 1 in a circle, the Fresnel lens layer 4 is arranged at an opening in the middle of the specimen carrier light source base 1, the light transmitting specimen carrier structure 5 is arranged on the upper surface of the Fresnel lens layer 4, the electronic objective lens 8 is arranged at the lower end of the electronic objective lens mounting threaded column 9, the electronic objective lens mounting threaded column 9 is in threaded connection with the middle of the upper end of the electronic objective lens mounting frame 7, and the lower end of the electronic objective lens mounting frame And the light-transmitting specimen cover plate 6 is positioned on the upper surface of the light-transmitting specimen carrier structure 5.
Further, the shape of printing opacity sample carrier structure 5 is the lid shape, the upper end edge of sample carrier light source base 1 is sunken to the inboard, 5 lid of printing opacity sample carrier structure are closed the upper end of sample carrier light source base 1 will the upper end of sample carrier light source base 1 the fresnel lens layer 4 the lamp strip formula light source 3 with light source reflection of light rete 2 all wraps up in sample carrier light source base 1.
Preferably, the edge of specimen carrier light source base 1 is provided with waterproof sealing ring 14, waterproof sealing ring 14 is located printing opacity specimen carrier structure 5 with between the specimen carrier light source base 1.
As an improvement, the lower end edge of the electronic objective mounting rack 7 is provided with a lower mounting rack magnetic attraction body 10, the edge of the specimen carrier light source base 1 is provided with an upper mounting rack magnetic attraction body 11 matched with the lower mounting rack magnetic attraction body 10, and the electronic objective mounting rack 7 is connected with the specimen carrier light source base 1 through the lower mounting rack magnetic attraction body 10 and the upper mounting rack magnetic attraction body 11 in a detachable manner.
As an improvement, the upper end edge of the specimen carrier light source base 1 is provided with a lower cover plate magnetic attraction body 12, the upper end edge of the light-transmitting specimen cover plate 6 is provided with an upper cover plate magnetic attraction body 13 matched with the lower cover plate magnetic attraction body 12, and the upper cover plate magnetic attraction body 13 is detachably and fixedly connected with the specimen carrier light source base 1 through the lower cover plate magnetic attraction body 12 and the upper cover plate magnetic attraction body 13.
The detection method of the intelligent automatic microorganism detection equipment comprises the following steps:
placing a microorganism specimen on the upper surface of a light-transmitting specimen carrier structure 5, and covering a light-transmitting specimen cover plate 6;
the lamp belt type light source 3 is electrified to emit light, the light is reflected to the Fresnel lens layer 4 through the light source reflecting film layer 2, and the Fresnel lens layer 4 uniformly scatters the light source to penetrate through the light-transmitting specimen carrier structure 5 and the light-transmitting specimen cover plate 6;
thirdly, butting the electronic objective mounting rack 7 with the specimen carrier light source base 1 to enable the microbial specimen to be positioned below the electronic objective 8;
fourthly, the electronic objective lens 8 can move up and down by spirally rotating the electronic objective lens mounting threaded column 9, so that the position of the electronic objective lens 8 is adjusted;
the signal of the electronic objective 8 is transmitted to a computer through a data line for detection and recording.
The working principle of the invention is as follows:
the connection between the electronic objective lens mounting threaded column 9 and the electronic objective lens mounting frame 7 can be manual, and the electronic objective lens mounting threaded column 9 can also be driven to rotate by a miniature motor, so that the electronic objective lens 8 can be electrically adjusted to lift, the light-transmitting specimen carrier structure 5 is in a cover shape, parts of an electronic light source, such as the Fresnel lens layer 4, the light strip type light source 3 and the light source reflecting film layer 2, are all coated in the light-transmitting specimen carrier structure 5, and the joint between the light-transmitting specimen carrier structure 5 and the specimen carrier light source base 1 is sealed by a waterproof sealing ring 14, so that when the light-transmitting specimen carrier structure 5 is cleaned, water is prevented from entering and damaging the light source; in addition, adopt upper cover plate magnetism to inhale body 13, lower cover plate magnetism to inhale body 12, lower mounting bracket magnetism to inhale body 10, go up the connected mode of mounting bracket magnetism to inhale body 11 for it is convenient to connect the dismantlement, and firm in connection does benefit to the operation that little biological detection.
The foregoing shows and describes the general principles and features of the present invention, together with the advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (6)

1. An intelligent automatic microorganism detection device, which is characterized in that: comprises a specimen carrier light source base (1), a light source reflecting film layer (2), a light belt type light source (3), a Fresnel lens layer (4), a light transmitting specimen carrier structure (5), a light transmitting specimen cover plate (6), an electronic objective lens mounting frame (7), an electronic objective lens (8) and an electronic objective lens mounting threaded column (9), wherein the light source reflecting film layer (2) is arranged at the bottom in the specimen carrier light source base (1), one light belt type light source (3) is arranged at one side edge in the specimen carrier light source base (1), the Fresnel lens layer (4) is arranged at an opening at the middle part of the specimen carrier light source base (1), the light transmitting specimen carrier structure (5) is arranged on the upper surface of the Fresnel lens layer (4), and the electronic objective lens (8) is arranged at the lower end of the electronic objective lens mounting threaded column (9), electron objective installation screw thread post (9) with the upper end middle part threaded connection of electron objective mounting bracket (7), the lower extreme of electron objective mounting bracket (7) with the connection can be dismantled at the edge of sample carrier light source base (1), printing opacity sample apron (6) are located the upper surface of printing opacity sample carrier structure (5).
2. The intelligent automated microbial detection apparatus of claim 1, wherein: the shape of printing opacity sample carrier structure (5) is the lid shape, the upper end edge of sample carrier light source base (1) is sunken to the inboard, printing opacity sample carrier structure (5) lid closes the upper end of sample carrier light source base (1), will the upper end of sample carrier light source base (1) chenille lens layer (4) lamp belt light source (3) with light source reflection of light rete (2) all cladding in sample carrier light source base (1).
3. The intelligent automated microbial detection apparatus of claim 2, wherein: the edge of sample carrier light source base (1) is provided with waterproof sealing washer (14), waterproof sealing washer (14) are located printing opacity sample carrier structure (5) with between sample carrier light source base (1).
4. The intelligent automated microbial detection apparatus of claim 1, wherein: the lower extreme edge of electron objective mounting bracket (7) is provided with down mounting bracket magnetism and inhales body (10), the edge of sample carrier light source base (1) be provided with last mounting bracket magnetism that lower mounting bracket magnetism is inhaled body (10) and is matchd inhales body (11), electron objective mounting bracket (7) with pass through between sample carrier light source base (1) down mounting bracket magnetism inhale body (10) with go up mounting bracket magnetism and inhale body (11) and can dismantle the connection.
5. The intelligent automated microbial detection apparatus of claim 1, wherein: the utility model discloses a specimen carrier light source base, including sample carrier light source base (1), the upper end edge of sample carrier light source base (1) is provided with down apron magnetism and attracts body (12), the upper end edge of printing opacity sample apron (6) be provided with apron magnetism is inhaled the upper cover plate magnetism that body (12) match down and is inhaled body (13), upper cover plate magnetism inhale body (13) with can dismantle fixed connection through apron magnetism and upper cover plate magnetism between sample carrier light source base (1) magnetism inhale body (12) and upper cover plate magnetism.
6. The intelligent automated microbial detection apparatus detection method of claim 1, comprising the steps of:
firstly, placing a microorganism specimen on the upper surface of a light-transmitting specimen carrier structure (5), and then covering a light-transmitting specimen cover plate (6);
the lamp belt type light source (3) is electrified to emit light, the light is reflected to the Fresnel lens layer (4) through the light source reflecting film layer (2), and the Fresnel lens layer (4) enables the light source to be uniformly scattered to penetrate through the light-transmitting specimen carrier structure (5) and the light-transmitting specimen cover plate (6);
thirdly, butting the electronic objective mounting rack (7) with the specimen carrier light source base (1) to enable the microbial specimen to be positioned below the electronic objective (8);
fourthly, the electronic objective lens is provided with the threaded column (9) through spiral rotation, so that the electronic objective lens (8) can move up and down, and the position of the electronic objective lens (8) is adjusted;
the signal of the electronic objective lens (8) is transmitted to a computer through a data line for detection and recording.
CN202011307255.4A 2020-11-19 2020-11-19 Intelligent automatic microorganism detection equipment and method thereof Pending CN112358960A (en)

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CN202011307255.4A CN112358960A (en) 2020-11-19 2020-11-19 Intelligent automatic microorganism detection equipment and method thereof

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Application Number Priority Date Filing Date Title
CN202011307255.4A CN112358960A (en) 2020-11-19 2020-11-19 Intelligent automatic microorganism detection equipment and method thereof

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CN112358960A true CN112358960A (en) 2021-02-12

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113267121A (en) * 2021-04-22 2021-08-17 西安交通大学医学院第一附属医院 Sample measuring device and method for tumor pathology

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200846640A (en) * 2007-05-31 2008-12-01 Contrel Technology Co Ltd Method of inspecting reflective backlight source and structure thereof
CN201540398U (en) * 2009-04-09 2010-08-04 田月皎 Microexamination device
CN110863032A (en) * 2019-12-05 2020-03-06 广东省生物工程研究所(广州甘蔗糖业研究所) Microorganism detection method
CN210419975U (en) * 2019-03-21 2020-04-28 全美基因(深圳)有限公司 Medical treatment is with biological gene composition analysis appearance

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200846640A (en) * 2007-05-31 2008-12-01 Contrel Technology Co Ltd Method of inspecting reflective backlight source and structure thereof
CN201540398U (en) * 2009-04-09 2010-08-04 田月皎 Microexamination device
CN210419975U (en) * 2019-03-21 2020-04-28 全美基因(深圳)有限公司 Medical treatment is with biological gene composition analysis appearance
CN110863032A (en) * 2019-12-05 2020-03-06 广东省生物工程研究所(广州甘蔗糖业研究所) Microorganism detection method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113267121A (en) * 2021-04-22 2021-08-17 西安交通大学医学院第一附属医院 Sample measuring device and method for tumor pathology

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