CN116554959A - Method for extracting high-quality euphausia superba oil from frozen euphausia superba - Google Patents
Method for extracting high-quality euphausia superba oil from frozen euphausia superba Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 43
- 241000239370 Euphausia superba Species 0.000 title description 25
- 229940106134 krill oil Drugs 0.000 claims abstract description 44
- 241000239366 Euphausiacea Species 0.000 claims abstract description 39
- 238000002386 leaching Methods 0.000 claims abstract description 31
- 238000000605 extraction Methods 0.000 claims abstract description 28
- 239000002904 solvent Substances 0.000 claims abstract description 28
- 238000000926 separation method Methods 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 24
- 238000011282 treatment Methods 0.000 claims description 20
- 239000011343 solid material Substances 0.000 claims description 16
- 238000010257 thawing Methods 0.000 claims description 12
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- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000011261 inert gas Substances 0.000 claims description 4
- 239000002798 polar solvent Substances 0.000 claims description 4
- 239000003208 petroleum Substances 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 abstract description 15
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- 230000000052 comparative effect Effects 0.000 description 15
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- 239000012535 impurity Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 3
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
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- 238000002137 ultrasound extraction Methods 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000239368 Euphausia Species 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 241000324401 Superba Species 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
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- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000007358 intestinal barrier function Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/14—Production of inert gas mixtures; Use of inert gases in general
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for extracting high-quality antarctic krill oil from frozen antarctic krill, belonging to the technical field of ocean functional food processing. The method adopts the direct extraction of the solvent, so as to quickly inactivate endogenous enzymes in the raw materials, avoid the hydrolysis of lipid and avoid the acid value of the final antarctic krill oil product to be too high; the influence of high moisture content of the frozen antarctic krill raw material on desolventizing of the leaching solution is solved through a membrane separation technology, and the method is simple and convenient, ensures the final antarctic krill oil product yield and avoids the excessively high peroxide value. The invention finally improves the yield and quality of the antarctic krill oil product through a multi-level optimization process. According to the invention, the frozen antarctic krill is taken as a raw material to directly extract oil, and the frozen antarctic krill is not prepared into powder and then extracted with oil, so that the cost is greatly saved and the resource waste is avoided. The method is an effective supplement for the existing production mode of the antarctic krill oil, and has important significance for improving the comprehensive high-value utilization degree of antarctic krill resources, especially frozen antarctic krill resources.
Description
Technical Field
The invention belongs to the technical field of ocean functional food processing, and particularly relates to a method for extracting high-quality antarctic krill oil from frozen antarctic krill.
Background
Euphausia superbaEuphausia superba) The method is a small crustacean zooplankton living in the Antarctic sea area, the biomass reaches 6.5 hundred million tons to 10 hundred million tons, and the method is a single type of trappable biological resource which is extremely abundant in resources and low in development and utilization degree and exists in the world at present. The antarctic krill has the nutrition characteristics of high lipid content and rich phospholipid polyunsaturated fatty acid, astaxanthin and other active lipid components, and is currently developed for the high-value utilization of antarctic krill resources at home and abroad by focusing on the production of lipid products such as antarctic krill oil. Compared with traditional marine oil products such as fish oil, the antarctic krill oil can play a better role in preventing and treating cardiovascular and cerebrovascular diseases, improving senile degenerative diseases, improving diabetes and complications, relieving sports fatigue, maintaining intestinal barrier function and the like, and has rapid market development.
The production modes of antarctic krill oil mainly comprise two modes: firstly, processing the fresh captured euphausia superba into euphausia superba powder by ship and then producing land-based euphausia superba oil, and secondly, directly extracting the euphausia superba oil by adopting frozen euphausia superba. Production modes for extracting antarctic krill oil by using antarctic krill powder have been developed to be mature and applied industrially, and the extraction of antarctic krill oil by using frozen antarctic krill has been slow to develop due to the defects of the prior art. However, on one hand, most of the captured antarctic krill still need to be transported back to land in the form of frozen antarctic krill for reuse at present due to the limitation of the processing capacity of the fishing processing ship; on the other hand, the whole demand of the consumer market cannot be met by only extracting and producing the antarctic krill oil by taking antarctic krill powder as a raw material.
Therefore, there is great interest in establishing a method for perfecting extraction of antarctic krill oil using frozen antarctic krill.
Disclosure of Invention
The invention aims to provide a method for extracting high-quality antarctic krill oil from frozen antarctic krill, which overcomes the defects of the prior art.
Because of the high moisture content (about 80% by weight) of frozen antarctic krill, the existing method for extracting and producing antarctic krill oil by using the same as a raw material still has a plurality of problems, such as: (1) If the frozen euphausia superba is processed into euphausia superba powder, and then the euphausia superba oil is obtained by solvent extraction, hot processing procedures such as cooking, drying and the like are needed, and problems such as material loss, lipid hydrolysis and/or oxidation and the like are easy to occur, so that the yield and quality of the final euphausia superba oil are affected; (2) If the raw materials are dehydrated by adopting physical modes such as squeezing and the like, the loss of the materials is easy to cause, and the lipid hydrolysis reaction is easy to be caused because endogenous enzymes are not inactivated, so that the finally obtained antarctic krill oil has high acid value; (3) The polar solvent is directly adopted to leach and freeze the euphausia superba, so that the problems caused by the hot processing procedures such as steaming, drying and the like can be avoided, endogenous enzymes can be denatured and deactivated to avoid lipid hydrolysis, but the water contained in the raw materials can be carried out along with leaching liquor during leaching, so that the subsequent desolventizing time is long, the bumping phenomenon is easy to occur, and in addition, the entrained water-soluble substances such as salt, protein, polysaccharide and the like also need to be removed.
According to the invention, the frozen antarctic krill is taken as a raw material to directly extract oil, the frozen antarctic krill is not made into powder, and the problems of material loss, lipid quality degradation and the like which are easily caused by processing procedures such as cooking, squeezing, drying and the like are avoided, but because the water content of the material is high, the water content can be brought out along with leaching liquor to cause adverse effects on subsequent desolventizing during direct leaching, and in addition, the entrained water-soluble impurities also need to be removed.
Aiming at the problems, the invention considers a membrane separation treatment method, mainly carries out interception treatment on the primary leaching extract 1, removes moisture and impurities and obtains an extract 2; leaching the solid material 1 to obtain an extracting solution 3; and combining the extracting solution 2 and the extracting solution 3 to obtain an extracting solution 4, and desolventizing to obtain the antarctic krill oil.
The invention adopts the following specific technical scheme:
a method for extracting high-quality antarctic krill oil from frozen antarctic krill comprises the following steps:
(1) After thawing the frozen antarctic krill raw material, carrying out solvent 1 leaching treatment, and carrying out solid-liquid separation after leaching to obtain an extracting solution 1 and a solid material 1;
(2) Performing membrane separation treatment on the extracting solution 1 to obtain extracting solution 2;
(3) Leaching the solid material 1 by using a solvent 2, and carrying out solid-liquid separation after leaching to obtain an extracting solution 3;
(4) And combining the extracting solution 2 and the extracting solution 3 to obtain an extracting solution 4, and desolventizing to obtain the antarctic krill oil.
Further, in the step (1), the thawing condition is: thawing still water, wherein the temperature of the still water is 10-15 ℃, and the thawing time is 20-60 min; the leaching conditions of the solvent 1 are as follows: the feed-liquid ratio is 1:3-1:15, the extraction temperature is 20-60 ℃, and the extraction time is 0.5-6 h; the solvent 1 is one or more of polar solvents, preferably ethanol solution with mass fraction of more than 95%.
Further, in the step (2), the membrane separation treatment conditions are as follows: the treatment pressure is 0.8 MPa to 1.4 MPa, the cycle treatment times are 3 times to 5 times, and the molecular weight cut-off range is 200 Da to 2000 Da.
Further, in the step (3), the solvent 2 leaching conditions are as follows: the feed-liquid ratio is 1:3-1:15, the extraction temperature is 20-60 ℃, the extraction time is 0.5-6 h, and the extraction times are 2-4 times; the solvent 2 is one or more of ethanol, n-hexane, petroleum ether, ethyl acetate, isopropanol and butanol.
Further, in the step (3), the solvent 2 may be used to pulp the solid material 1 in advance by using a refiner.
Further, in the step (3), the solvent 2 may be extracted with an inert gas; the inert gas is nitrogen.
Further, in the step (3), ultrasonic assistance may be used for leaching the solvent 2; the ultrasonic conditions are as follows: the ultrasonic power is 100-600W, the ultrasonic temperature is 20-40 ℃, and the ultrasonic time is 5-30 min.
Further, in the step (4), the desolventizing condition is: the temperature is 25-50 ℃ and the vacuum degree is-0.05 MPa to-0.1 MPa.
Further, in the step (4), the extracting solution 4 may be subjected to centrifugation before the desolventizing treatment, so as to further remove insoluble impurities; the centrifugation conditions are as follows: the rotating speed is 7500 r/min-15000 r/min, and the time is 5 min-15 min.
Compared with the prior art, the invention has the advantages that:
the method adopts the direct leaching of the solvent, so as to quickly denature and inactivate endogenous enzymes in the raw materials, avoid the hydrolysis and the destruction of beneficial lipid components such as polyunsaturated fatty acid phospholipids, astaxanthin esters and the like, and avoid the acid value of the final antarctic krill oil product to be too high; the method does not need to inactivate endogenous enzymes by adopting a cooking heating mode used in the prior art, and avoids material loss caused by cooking; the influence of high moisture content of the frozen antarctic krill raw material on desolventizing of leaching liquid is solved through a membrane separation technology, the leaching liquid is simple and convenient, the raw material is not required to be dehydrated in a squeezing mode, a drying mode and the like used in the prior art, the material loss caused by squeezing is avoided, the lipid oxidation caused by drying is avoided, a multi-level optimization process is adopted, and finally the yield and the quality of antarctic krill oil products are improved.
According to the invention, before extraction treatment, the solid materials are homogenized so as to enable the materials to fully contact with the leaching solvent, ultrasonic auxiliary extraction can be adopted in the extraction process, the extraction time is shortened, the dissolution efficiency is improved, and the yield of the final antarctic krill oil product is further ensured. In addition, inert gas is introduced in the extraction process to protect, so that the polyunsaturated fatty acid, astaxanthin and other lipids are prevented from being oxidized, and the quality of the final antarctic krill oil product is further ensured.
According to the invention, the production process of the antarctic krill oil is optimized, the frozen antarctic krill is taken as a raw material to directly extract oil, the frozen antarctic krill is not prepared into powder and then extracted with oil, the cost is greatly saved, and the resource waste is avoided. The method is an effective supplement for the existing production mode of the antarctic krill oil, and has important significance for improving the comprehensive high-value utilization degree of antarctic krill resources, especially frozen antarctic krill resources.
Drawings
Fig. 1 is a process flow diagram of the present invention.
Detailed Description
The technical scheme of the invention is further described and illustrated below by combining with the embodiment.
Example 1
A method for extracting high-quality antarctic krill oil from frozen antarctic krill, as shown in fig. 1, comprising the following steps:
(1) Thawing 500 g frozen Euphausia superba in still water at 15 ℃ for 30 min, leaching with ethanol solution with mass fraction of 95% or more according to a feed-liquid ratio of 1:3 and an extraction temperature of 20 ℃ for 4.5 h, and performing solid-liquid separation to obtain extract 1 and solid material 1;
(2) Performing membrane separation treatment on the extracting solution 1 under the pressure of 1.2 MPa, circularly treating for 3 times, and intercepting and recovering components with the molecular weight range of 200 Da to 2000 Da to obtain extracting solution 2;
(3) Extracting solid material 1 with ethanol at 20deg.C for 4.5 h at a ratio of 1:3 for 3 times, mixing extractive solutions, and performing solid-liquid separation to obtain extractive solution 3;
(4) Mixing the above extractive solutions 2 and 3 to obtain extractive solution 4, and desolventizing at 45deg.C under vacuum degree of-0.05 MPa to obtain Euphausia superba oil.
Example 2
A method for extracting high-quality antarctic krill oil from frozen antarctic krill comprises the following steps:
(1) Thawing 10 kg frozen Euphausia superba in still water at 15 ℃ for 60 min, leaching with ethanol solution with mass fraction of 95% or more according to a feed-liquid ratio of 1:3 and an extraction temperature of 20 ℃ for 4.5 h, and performing solid-liquid separation to obtain extract 1 and solid material 1;
(2) Performing membrane separation treatment on the extracting solution 1 under the pressure of 1.2 MPa, circularly treating for 3 times, and intercepting and recovering components with the molecular weight range of 200 Da to 2000 Da to obtain extracting solution 2;
(3) Extracting solid material 1 with ethanol at 20deg.C for 4.5 h at a ratio of 1:3 for 3 times, mixing extractive solutions, and performing solid-liquid separation to obtain extractive solution 3;
(4) Mixing the above extractive solutions 2 and 3 to obtain extractive solution 4, and desolventizing at 45deg.C under vacuum degree of-0.05 MPa to obtain Euphausia superba oil.
Example 3
A method for extracting high-quality antarctic krill oil from frozen antarctic krill comprises the following steps:
(1) Thawing 500 g frozen Euphausia superba in still water at 15 ℃ for 30 min, leaching with ethanol solution with mass fraction of 95% or more according to a feed-liquid ratio of 1:3 and an extraction temperature of 20 ℃ for 4.5 h, and performing solid-liquid separation to obtain extract 1 and solid material 1;
(2) Performing membrane separation treatment on the extracting solution 1 under the pressure of 1.2 MPa, circularly treating for 3 times, and intercepting and recovering components with the molecular weight range of 200 Da to 2000 Da to obtain extracting solution 2;
(3) Homogenizing a solid material 1 by a homogenizer, adding ethanol according to a feed-liquid ratio of 1:3, performing ultrasonic treatment for 15 min under the conditions of nitrogen protection, ultrasonic power of 300W and ultrasonic temperature of 20 ℃, performing leaching under the condition of nitrogen protection according to the conditions of extraction temperature of 20 ℃ and extraction time of 2 h, repeatedly extracting for 3 times, and combining the extracting solutions to perform solid-liquid separation to obtain an extracting solution 3;
(4) Mixing the above extractive solutions 2 and 3 to obtain extractive solution 4, centrifuging at 12000/r/min for 10 min, and desolventizing supernatant at 45deg.C under vacuum degree of-0.05 MPa to obtain Euphausia superba oil.
Example 4
A method for extracting high-quality antarctic krill oil from frozen antarctic krill comprises the following steps:
(1) Thawing 10 kg frozen Euphausia superba in still water at 15 ℃ for 60 min, leaching with ethanol solution with mass fraction of 95% or more according to a feed-liquid ratio of 1:3 and an extraction temperature of 20 ℃ for 4.5 h, and performing solid-liquid separation to obtain extract 1 and solid material 1;
(2) Performing membrane separation treatment on the extracting solution 1 under the pressure of 1.2 MPa, circularly treating for 3 times, and intercepting and recovering components with the molecular weight range of 200 Da to 2000 Da to obtain extracting solution 2;
(3) Homogenizing a solid material 1 by a homogenizer, adding ethanol according to a feed-liquid ratio of 1:3, performing ultrasonic treatment for 15 min under the conditions of nitrogen protection, ultrasonic power of 300W and ultrasonic temperature of 20 ℃, performing leaching under the condition of nitrogen protection according to the conditions of extraction temperature of 20 ℃ and extraction time of 2 h, repeatedly extracting for 3 times, and combining the extracting solutions to perform solid-liquid separation to obtain an extracting solution 3;
(4) Mixing the above extractive solutions 2 and 3 to obtain extractive solution 4, centrifuging at 12000/r/min for 10 min, and desolventizing supernatant at 45deg.C under vacuum degree of-0.05 MPa to obtain Euphausia superba oil.
In another embodiment, the solvent 1 may be an ethanol solution with a mass fraction of 95% or more, or one or more of polar solvents may be selected and mixed; the solvent 2 is one or more of ethanol, n-hexane, petroleum ether, ethyl acetate, isopropanol and butanol. The solvent is selected as long as the solvent meets the purpose of the invention, and belongs to equivalent substitution, and the solvent is within the protection scope of the invention.
Comparative example 1:
an extraction method of antarctic krill oil comprises the following steps: 500 The g frozen euphausia superba is directly prepared into powder by vacuum freeze drying, and then the total lipid is extracted by adopting a Folch method to obtain euphausia superba oil.
Comparative example 2:
the comparative example adopts the technical scheme of patent 'method for extracting krill oil with high phospholipid content from fresh antarctic krill' (patent number ZL 201410777763.7), and takes 500 g frozen antarctic krill to obtain antarctic krill oil.
Comparative example 3:
the comparative example adopts the technical scheme of patent 'method for extracting high-quality shrimp oil from antarctic krill and preparing defatted krill protein powder' (patent number ZL 201110447702.0) to obtain antarctic krill oil by taking 500 g frozen antarctic krill.
The antarctic krill oil obtained in the above examples and comparative examples was subjected to yield and quality measurements, the results of which are shown in the following table:
table 1 comparative examples and comparative examples of Antarctic krill oil yield
As can be seen from Table 1, the yield of the extraction of the antarctic krill oil from the frozen antarctic krill powder obtained in comparative example 1 is highest by directly adopting vacuum freeze-drying, but the method has high energy consumption and the used leaching solvents, namely methanol and chloroform, have high toxicity, are only suitable for preparing small samples through laboratory scientific research, and are not suitable for large-scale production. The yields of the examples 1 and 3 are also higher and approach to the comparative example 1, and the examples 2 and 4 are obtained by extracting the raw materials amplified 20 times on the basis of the examples 1 and 3, and the yields are close to the yields of the examples 1 and 3, so that the preparation method provided by the invention can be applied to the mass production of the antarctic krill oil from the aspect of practical application feasibility; and in the embodiments 3 and 4, the treatments of material homogenization, nitrogen protection, ultrasonic extraction and the like are added on the basis of the embodiments 1 and 2, so that the yield of the antarctic krill oil can be further improved. The antarctic krill oil prepared in comparative examples 2 and 3 has lower yield, which is related to material loss caused by the procedures of cooking, squeezing and the like involved in the corresponding technical scheme, and the preparation method provided by the invention effectively avoids the problems.
Table 2 quality comparison of antarctic krill oil prepared in examples and comparative examples
As can be seen from table 2, the comparison of examples 1-4 and comparative example 1 yields a final product of example 1-4 with quality of antarctic krill oil that is close to the original lipid quality of the frozen antarctic krill material and significantly better than comparative example 2. And in the embodiments 3 and 4, the treatments of material homogenization, nitrogen protection, ultrasonic extraction and the like are added on the basis of the embodiments 1 and 2, so that the quality of the antarctic krill oil can be further ensured.
By comparing the examples 1-4 with the comparative examples 2-3, the technical scheme of the invention can effectively avoid the problems of material loss, lipid hydrolysis, lipid oxidation and the like which are easily caused by the processing procedures of cooking, squeezing, drying and the like in the prior art, can effectively remove water and water-soluble impurities, and ensures the yield of the final euphausia superba oil product and improves the quality.
The present invention has been described in detail with reference to the above embodiments, and the functions and actions of the features in the present invention will be described in order to help those skilled in the art to fully understand the technical solution of the present invention and reproduce it.
Finally, although the description has been described in terms of embodiments, not every embodiment is intended to include only a single embodiment, and such description is for clarity only, as one skilled in the art will recognize that the embodiments of the disclosure may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (10)
1. A method for extracting high-quality antarctic krill oil from frozen antarctic krill, which is characterized by comprising the following steps:
(1) After thawing the frozen antarctic krill raw material, carrying out solvent 1 leaching treatment, and carrying out solid-liquid separation after leaching to obtain an extracting solution 1 and a solid material 1;
(2) Performing membrane separation treatment on the extracting solution 1 to obtain extracting solution 2;
(3) Leaching the solid material 1 by using a solvent 2, and carrying out solid-liquid separation after leaching to obtain an extracting solution 3;
(4) And combining the extracting solution 2 and the extracting solution 3 to obtain an extracting solution 4, and desolventizing to obtain the antarctic krill oil.
2. The method of claim 1, wherein in step (1), the thawing conditions are: thawing still water, wherein the temperature of the still water is 10-15 ℃, and the thawing time is 20-60 min; the conditions for leaching the solvent 1 are as follows: the feed-liquid ratio is 1:3-1:15, the extraction temperature is 20-60 ℃, and the extraction time is 0.5-6 h; the solvent 1 is one or more of polar solvents.
3. The method according to claim 2, wherein in the step (1), the solvent 1 is an ethanol solution having a mass fraction of 95% or more.
4. The method of claim 1, wherein in step (2), the membrane separation process conditions are: the treatment pressure is 0.8 MPa to 1.4 MPa, the cycle treatment times are 3 times to 5 times, and the molecular weight cut-off range is 200 Da to 2000 Da.
5. The method of claim 1, wherein in step (3), the solvent 2 leaching conditions are: the feed-liquid ratio is 1:3-1:15, the extraction temperature is 20-60 ℃, the extraction time is 0.5-6 h, and the extraction times are 2-4 times; the solvent 2 is one or more of ethanol, n-hexane, petroleum ether, ethyl acetate, isopropanol and butanol.
6. The method of claim 1, wherein in step (3), the solvent 2 is used to pulp the solid material 1 in advance by using a refiner.
7. The method of claim 1, wherein in step (3), the solvent 2 is extracted with an inert gas.
8. The method of claim 1, wherein in step (3), the solvent 2 is leached with ultrasonic assistance; the ultrasonic conditions are as follows: the ultrasonic power is 100-600W, the ultrasonic temperature is 20-40 ℃, and the ultrasonic time is 5-30 min.
9. The method of claim 1, wherein in step (4), the desolventizing conditions are: the temperature is 25-50 ℃ and the vacuum degree is-0.05 MPa to-0.1 MPa.
10. The method according to claim 1, wherein in the step (4), the extract 4 is subjected to centrifugation before the desolventizing treatment; the centrifugation conditions are as follows: the rotating speed is 7500 r/min-15000 r/min, and the time is 5 min-15 min.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104479850A (en) * | 2014-12-15 | 2015-04-01 | 中国水产科学研究院黄海水产研究所 | Method for extracting krill oil with high phospholipid content from fresh Antarctic krill |
| CN107760439A (en) * | 2017-11-01 | 2018-03-06 | 浙江海洋大学 | A kind of method that ultrasonic assistant ethanol extraction prepares antarctic krill oil |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104479850A (en) * | 2014-12-15 | 2015-04-01 | 中国水产科学研究院黄海水产研究所 | Method for extracting krill oil with high phospholipid content from fresh Antarctic krill |
| CN107760439A (en) * | 2017-11-01 | 2018-03-06 | 浙江海洋大学 | A kind of method that ultrasonic assistant ethanol extraction prepares antarctic krill oil |
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| 孙甜甜: "高品质南极磷虾虾油工业化生产技术研究", 《中国优秀硕士学位论文全文数据库电子期刊》, pages 52 * |
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