CN104479859A - Preparation method of roe extract - Google Patents
Preparation method of roe extract Download PDFInfo
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- CN104479859A CN104479859A CN201410725549.7A CN201410725549A CN104479859A CN 104479859 A CN104479859 A CN 104479859A CN 201410725549 A CN201410725549 A CN 201410725549A CN 104479859 A CN104479859 A CN 104479859A
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- fish
- egg
- ovum
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention provides a preparation method of a roe extract. The preparation method comprises the following steps: directly performing static digestion on roes after freeze-drying, and then removing a solvent from the extracting solution to obtain the roe extract. According to the preparation method provided by the invention, dried roes do not need to be crushed or (and) puffed and granulated, so that a process is simple, dust pollution in a production process is prevented, and the quality of the roe extract can be improved.
Description
Technical field
The present invention relates to field of marine biotechnology, particularly relate to a kind of preparation method of fish-egg extract.
Background technology
Fish-egg is one of domestic and international dark ace-high cuisines, it contains rich in protein, lipid, VITAMIN and mineral substance, and the main component of its extract is the lipid composition such as triglyceride level (13.1%-56.9%), phosphatide (30.2%-80.3%) and liposoluble vitamin.In the triglyceride level of fish-egg extract, DHA content is 3.2%-15.3%, and its chemical structure is identical with common DHA product (fish oil, micro-algae oil).The phosphatide contained in fish-egg extract is the special phosphatide be combined with ester bond with DHA, its DHA content is 21.4%-37.2%, be different from the physical mixture of DHA and soybean (or yolk) phosphatide, this kind of phosphatide has the dual physiological function of DHA and phosphatide, its lysophospholipid generating free fatty acids at human body and be rich in DHA under the effect of pancreas Phospholipid hydrolase, the latter enters small intestinal cell by cytolemma, be combined with serum albumin afterwards, be transported to the organs such as brain and efficiently combine with it.Research finds, in fish-egg extract, the efficiency digested and assimilated and be combined with brain of DHA is far above common DHA product, and therefore fish-egg extract can be used as the large good function composition supplementing brain and neurocyte DHA.
The preparation method of usual fish-egg extract is by fish-egg crushed after being dried, utilizes organic solvent or supercutical fluid to extract as extraction agent, then removes extraction agent and obtain fish-egg extract, and the difficult point of the method is the globule size controlling fish-egg pulverizing.Grinding particle size too conference causes the lipid composition loose contact in extraction agent and fish-egg, thus makes the yield of extract very low; Grinding particle size is too small, easily causes the oxidation of transport and storage fish-egg lipid composition, and easily causes flying upward of fish-egg powder in transport and leaching process, causes the dust pollution of environment.
Patent application 201410089039.5 discloses a kind of method of Extractive technology phosphatide from aquatic animal ovum, to mix with starch after the fish-egg of drying being pulverized in its leaching process and carry out expanded granulation, carry out the ethanol extraction that lixiviate and solvent removal obtain aquatic animal ovum afterwards.Although the method obtains very high extraction yield, but still fish-egg need be pulverized, and owing to adding starch, causes the utility value of the alcohol-insoluble substance extracting rear rich in proteins greatly to reduce.
Summary of the invention
The invention provides a kind of preparation method of fish-egg extract, to solve the problems of the technologies described above, it directly carries out static lixiviate by after fish-egg lyophilize, then obtains fish-egg extract by after extracting solution desolvation.
Further, described method comprises:
The first step, by freezing fish-egg in-10 DEG C ~-50 DEG C, under 10Pa ~ 200Pa condition lyophilize to water content less than 10%;
Second step, be placed in by dry for the first step gained fish-egg and extract still, 90% ~ 100% ethanol adding 3 ~ 15 times of dry fish-egg weight carries out static lixiviate, and its extraction temperature is 10 DEG C ~ 50 DEG C, extraction time 4-16 hour;
3rd step, by extracting solution 40 DEG C ~ 80 DEG C, carry out underpressure distillation desolvation under the condition of-0.070Mpa ~-0.095Mpa after obtain fish-egg extract.
Described fish-egg is the ovum deriving from marine fishes, includes but not limited to squid ovum, catfish ovum, cod roe, flying fish ovum, salmon roe and SHISHAMO ovum.
Under normal circumstances, freezing fish-egg is after ordinary method drying (as warm air drying), the surface of fish-egg there will be obvious sclerosis on the one hand, on the other hand, fish-egg volume has and significantly reduces, and forms fine and close fish-egg particle, when preparing the ethanol extraction of fish-egg, must be pulverized, to ensure that ethanol and the lipid composition in fish-egg are able to fully contact, thus be obtained higher extraction yield.
Freeze Drying Technique is frozen into solid-state at a lower temperature by wet stock or solution, then makes moisture wherein be directly sublimed into gaseous state without liquid state under vacuo, finally make the dry technology of material dewatering.Drying due to fish-egg completes in the frozen state, compared with other drying meanss, physical structure and the molecule structure change of material are minimum, the weave construction of fish-egg and mode of appearance are preserved preferably, and in vacuum lyophilization process, there is not surface hardening problem in fish-egg, its inside can form the spongy of porous again, for the derect seething of fish-egg lipid provides a great convenience.
Fish-egg is after lyophilize, mode of appearance when can it be kept preferably freezing on the one hand, the expanded granulating working procedure adopted in patent application 201410089039.5 can be omitted, on the other hand, fish-egg is in freezing dry process, all the time under the state being in low temperature and anaerobic, the how unsaturated oxidation at lipid acid in fish-egg lipid can be reduced, improve the quality level of extract.
Beneficial effect
The preparation method of a kind of fish-egg extract provided by the invention, without the need to dried fish-egg is carried out pulverization process or (with) expanded granulation, technique is simple, avoids the dust pollution in production process, and can improve the quality of product.
Embodiment
The invention provides a kind of preparation method of fish-egg extract, it directly carries out static lixiviate by after fish-egg lyophilize, then obtains fish-egg extract by after extracting solution desolvation.Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how utilisation technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1
Take 1000g frozen squid ovum in-28 DEG C, lyophilize 24h under 50Pa condition, obtain the dry squid ovum 233.5g that water content is 4%, being placed on 3L extracts in still, add 1.8kg95% ethanol and make its complete submergence squid ovum, static lixiviate released vat liquor after 6 hours, by vat liquor underpressure distillation (60 DEG C ,-0.09Mpa), obtain squid Egg extracts 110.3g.
Embodiment 2
Take the freezing catfish ovum of 1000g in-30 DEG C, lyophilize 36h under 43Pa condition, obtain the dry catfish ovum 315.0g that water content is 2%, being placed on 5L extracts in still, add 3kg dehydrated alcohol and make its complete submergence catfish ovum, static lixiviate released vat liquor after 12 hours, by vat liquor underpressure distillation (60 DEG C ,-0.09Mpa), obtain catfish Egg extracts 45.9g.
Comparative example 1
Take after 1000g frozen squid ovum naturally thaws under room temperature condition, warm air drying (80 DEG C, 12 hours) obtain dry catfish ovum 234.1g, being crushed to granularity 100-300 order is placed in 3L extraction still, add 1.8kg 95% ethanol, stir extraction and after 6 hours, filter the vat liquor obtaining clarifying, obtain catfish extract 111.2g by after vat liquor underpressure distillation (60 DEG C ,-0.09Mpa).
Comparative example 2
Take after the freezing catfish ovum of 1000g naturally thaws under room temperature condition, warm air drying (80 DEG C, 12 hours) obtain dry catfish ovum 312.5g, being crushed to granularity 100-300 order is placed in 5L extraction still, add 3kg dehydrated alcohol, stir extraction and after 12 hours, filter the vat liquor obtaining clarifying, obtain catfish extract 46.2g by after vat liquor underpressure distillation (60 DEG C ,-0.09Mpa).
Measure phospholipids content (GB/T 5537), peroxide value (GB/T 5538) and acid number (GB/T 5530) in above-described embodiment and comparative example gained fish-egg extract, measurement result is as shown in table 1:
Phospholipids content and peroxide value in table 1 fish-egg extract
More known by embodiment and comparative example, comparative example adopts the method for warm air drying-pulverizing-extraction to prepare fish-egg extract, although time of drying, short and extraction yield was a little more than embodiment, but the peroxide value in embodiment product and acid number are starkly lower than comparative example, therefore method provided by the invention may be used for preparing high-quality fish-egg extract.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (2)
1. a preparation method for fish-egg extract, is characterized in that, described method comprises:
The first step, by freezing fish-egg in-10 DEG C ~-50 DEG C, under 10Pa ~ 200Pa condition lyophilize to water content less than 10%;
Second step, be placed in by dry for the first step gained fish-egg and extract still, 90% ~ 100% ethanol adding 3 ~ 15 times of dry fish-egg weight carries out static lixiviate, and its extraction temperature is 10 DEG C ~ 50 DEG C, extraction time 4-16 hour;
3rd step, by extracting solution 40 DEG C ~ 80 DEG C, carry out underpressure distillation desolvation under the condition of-0.070Mpa ~-0.095Mpa after obtain fish-egg extract.
2. the preparation method of a kind of fish-egg extract as claimed in claim 1, it is characterized in that, described fish-egg is the ovum deriving from marine fishes, includes but not limited to squid ovum, catfish ovum, cod roe, flying fish ovum, salmon roe and SHISHAMO ovum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410725549.7A CN104479859A (en) | 2014-11-27 | 2014-11-27 | Preparation method of roe extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410725549.7A CN104479859A (en) | 2014-11-27 | 2014-11-27 | Preparation method of roe extract |
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| CN104479859A true CN104479859A (en) | 2015-04-01 |
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| CN201410725549.7A Pending CN104479859A (en) | 2014-11-27 | 2014-11-27 | Preparation method of roe extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489833A (en) * | 2016-10-31 | 2017-03-15 | 威海深蓝奇迹生物科技有限公司 | Feed addictive and its feed and method rich in DHA phosphatide |
| CN109805156A (en) * | 2019-03-18 | 2019-05-28 | 威海深蓝奇迹生物科技有限公司 | A kind of preparation method of the yolk powder of DHA containing phosphatide type |
| CN110664724A (en) * | 2019-11-21 | 2020-01-10 | 广州美颂化妆品有限公司 | Skin care product raw material containing roe extract for moisturizing, removing wrinkles and removing red blood streak and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548782A (en) * | 2008-04-01 | 2009-10-07 | 浙江中医药大学 | Method for extracting fish oil by utilizing mackerel heads or squid viscera |
| CN102492538A (en) * | 2011-11-14 | 2012-06-13 | 辽宁省大连海洋渔业集团公司 | Method for extracting Antarctic krill oil with high phosphatide content from Antarctic krill |
| CN103864841A (en) * | 2014-03-12 | 2014-06-18 | 威海博宇食品有限公司 | Method for extracting and refining phospholipid from egg of aquatic animal |
-
2014
- 2014-11-27 CN CN201410725549.7A patent/CN104479859A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548782A (en) * | 2008-04-01 | 2009-10-07 | 浙江中医药大学 | Method for extracting fish oil by utilizing mackerel heads or squid viscera |
| CN102492538A (en) * | 2011-11-14 | 2012-06-13 | 辽宁省大连海洋渔业集团公司 | Method for extracting Antarctic krill oil with high phosphatide content from Antarctic krill |
| CN103864841A (en) * | 2014-03-12 | 2014-06-18 | 威海博宇食品有限公司 | Method for extracting and refining phospholipid from egg of aquatic animal |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489833A (en) * | 2016-10-31 | 2017-03-15 | 威海深蓝奇迹生物科技有限公司 | Feed addictive and its feed and method rich in DHA phosphatide |
| CN106489833B (en) * | 2016-10-31 | 2018-03-27 | 威海深蓝奇迹生物科技有限公司 | Feed addictive and its feed and method rich in DHA phosphatide |
| CN109805156A (en) * | 2019-03-18 | 2019-05-28 | 威海深蓝奇迹生物科技有限公司 | A kind of preparation method of the yolk powder of DHA containing phosphatide type |
| CN110664724A (en) * | 2019-11-21 | 2020-01-10 | 广州美颂化妆品有限公司 | Skin care product raw material containing roe extract for moisturizing, removing wrinkles and removing red blood streak and preparation method thereof |
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