CN116549514A

CN116549514A - Traditional Chinese medicine composition for treating fish enteritis

Info

Publication number: CN116549514A
Application number: CN202310700263.2A
Authority: CN
Inventors: 赵宇杰; 严永; 程安达; 王秀敏; 孙艳霞; 江厚生
Original assignee: Centre Inner Mongolia Technology Co ltd; Beijing Centre Biology Co ltd
Current assignee: Centre Inner Mongolia Technology Co ltd; Beijing Centre Biology Co ltd
Priority date: 2023-06-14
Filing date: 2023-06-14
Publication date: 2023-08-08

Abstract

The invention discloses a Chinese herbal medicine composition for treating fish enteritis, which is prepared from the following traditional Chinese medicinal materials, by weight, 20-30 parts of common andrographis herb, 20-30 parts of Chinese mahonia, 15-25 parts of humifuse euphorbia herb, 15-25 parts of giant knotweed rhizome, 15-25 parts of baical skullcap root, 10-20 parts of astragalus root and 10-20 parts of tree peony bark. The traditional Chinese medicine composition reasonably combines traditional Chinese medicine raw materials, has the functions of clearing heat and drying dampness, detoxifying and stopping diarrhea and inhibiting pathogenic microorganisms, has no toxic or side effect, obvious curative effect, low cost, convenient use and wide market prospect, is used for treating enteritis of aquatic animals, and reduces loss caused by enteritis in the cultivation process.

Description

Traditional Chinese medicine composition for treating fish enteritis

Technical Field

The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a traditional Chinese medicine composition for treating fish enteritis and a preparation method thereof.

Background

Fish growth is closely related to intestinal health. In aquaculture, high-culture-density feeding is extremely easy to cause serious stress of fish, so that the incidence rate of enteritis of the fish is greatly increased, the occurrence of enteritis often causes disturbance of intestinal immune function, and the growth of the fish is reduced. In recent years, as farmers blindly pursue the yield of freshwater fish, the breeding density is improved, and the large-area popularization and application of pellet feed are realized, and the occurrence of fish diseases is relatively high. The most common of these diseases is enteritis, which has been one of the common diseases in pond fish production. The enteritis death rate of grass carp is very high, generally 30% -60%, and can reach more than 95% seriously, if the grass carp cannot be treated in time, a large amount of fish can die, and great economic loss is brought to raisers. According to the pathogenesis, epidemic time, clinical symptoms and the like of grass carp enteritis, the grass carp enteritis can be divided into viral enteritis, bacterial enteritis, mechanical injury enteritis and other types:

(1) Viral enteritis

Etiology and time of epidemic: the pathogen is reovirus. Mainly endangering fingerling in the current year and sometimes grass carp of insufficient age also occurs. The disease has long season of onset, epidemic season at the end of last 6 months to 9 months each year, peak period of onset at 8 months, water temperature of 20-33 ℃ is easy to be popular, water temperature of 25-30 ℃ is most suitable, and the disease is most easy to be suffered when the water quality is deteriorated, the dissolved oxygen in water is low, the water temperature change is large, the transparency of water is low and the oxygen consumption of organic matters is high.

Clinical symptoms and diagnosis: the congestion of body surface and muscle is not obvious, but the congestion of intestinal canal is serious, and the intestine is not provided with food. The intestinal tract is red or purple in all or part, and the mesentery and the wall of the air bladder have punctiform congestion. The disease is obvious in congestion of body surface and muscle parts when the disease occurs simultaneously with the red muscle type and the red fin gill cap type. Taking intestinal mucus for microscopic examination, the intestinal epithelium is peeled off from the lamina propria, and the intestinal epithelium is completely peeled off in severe cases, and the lamina propria and submucosa have massive hemorrhage, so that the intestinal epithelium is swollen. The diagnosis can be confirmed by using an immunological diagnosis method such as an enzyme-linked immunosorbent assay, an immunoperoxidase technique, a staphylococcus protein A synergic agglutination assay and the like.

(2) Bacterial enteritis

Etiology and time of epidemic: the main pathogen is aeromonas hydrophila. Bacterial enteritis is commonly called as intestinal rot, is one of the most serious diseases in grass carp production, can occur from fingerling to adult fish, begins to prevail when the water temperature reaches more than 18 ℃, is in a peak period when the water temperature is 25-30 ℃, is mostly developed in 7-9 months in the year, and is mostly developed in 5-6 months in the 1-year old fish.

Clinical symptoms and diagnosis: the abdomen is enlarged, erythema is usually arranged on two sides, the red swelling and the outward protruding of the anus are purple red, and yellow mucus and bloody pus flow out when the abdomen is lightly pressed. The intestinal cavity is free of food or has a small amount of food at the rear section of the intestine, the intestinal wall is weak in elasticity and is easy to break, and the intestinal mucosa is often ulcerated and inflamed. The intestinal mucus was examined microscopically and denatured, necrotic shed intestinal epithelial cells and small numbers of erythrocytes were seen. The liver, spleen, kidney and heart blood of the diseased fish are inoculated on the R-s selection and identification medium, and if yellow colonies grow, the disease can be diagnosed as bacterial enteritis.

(3) Mechanical injury enteritis

Etiology and time of epidemic: improper feeding or feeding of straw grass, rice, etc. are the main causes of morbidity. Grass carp belongs to stomach-free fish, and is fast to eat and greedy to eat because of large eating amount, after entering summer, the activity and eating ability of the grass carp are increased, a producer pursues the growth speed once, the feeding frequency is increased blindly, the feeding amount is increased, the intestinal tract is excessively full, the digestive function is always in a state of high excitation, mechanical damage of the intestinal tract is caused over time, or the intestinal wall is directly stabbed by fed straw grass or rice, so that the high-temperature season is the high-incidence time of mechanical damage of the intestinal tract of the grass carp.

Clinical symptoms and diagnosis: under the conditions that parasites such as naea, the clarias, and the like on the body surface of the diseased fish are not present, and the external environment is not disturbed, the pool fish is suddenly frightened when feeding, and rapidly flees around or drills into the bottom of the pool, and continuously returns to the food field to frighten and rob to eat when feeding is continued, and the pool is fried for a plurality of times. About one week, the death of fish begins, often with a relatively large early death of the individual. The appearance of the fish in the initial stage of disease is characterized by no abnormal appearance, and along with the further development of the disease, the fish eye balls are obviously sunken, the fish body seriously loses water, and the body color is blackened. Anatomical analysis can lead to redness and inflammation of the anterior segment of the intestinal tract, and congestion or suppuration of the intestinal tract in severe cases.

At present, chemical medicines with stronger irritation are mostly adopted for treating the enteritis of fish, but the unreasonable use of the medicines causes continuous enhancement of the drug resistance of pathogenic bacteria, increased dosage, continuous cure and continuous decline of prevention and treatment effects. Meanwhile, the excessive medicine residue also causes the problems of potential harm to human health, environmental pollution, food safety and the like of aquatic animals. The Chinese herbal medicine has the advantages of naturalness, multifunction, small toxic and side effects and no drug resistance, meets the industry requirements of green cultivation advocated in the current society, and has wide development prospect. Therefore, the development of the medicine which takes the Chinese herbal medicines as the raw materials and is used for treating the bacterial enteritis of the fish has important significance and provides powerful guarantee for the healthy development of the aquaculture industry.

Disclosure of Invention

In a first aspect, the invention provides a traditional Chinese medicine composition for treating fish enteritis, which is prepared from the following traditional Chinese medicinal materials, by weight, 20-30 parts of common andrographis herb, 20-30 parts of Chinese mahonia, 15-25 parts of humifuse euphorbia herb, 15-25 parts of giant knotweed rhizome, 15-25 parts of baical skullcap root, 10-20 parts of astragalus mongholicus and 10-20 parts of cortex moutan.

Preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials, by weight, 20-25 parts of common andrographis herb, 20-25 parts of Chinese mahonia, 15-20 parts of humifuse euphorbia herb, 15-20 parts of giant knotweed, 15-20 parts of baical skullcap root, 10-15 parts of astragalus mongholicus and 10-15 parts of cortex moutan.

Preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials in parts by weight, namely 25 parts of common andrographis herb, 25 parts of Chinese mahonia, 20 parts of humifuse euphorbia herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 10 parts of astragalus mongholicus and 10 parts of cortex moutan.

Further, the fish include, but are not limited to, grass carp, black carp, crucian carp, and the like.

Further, the fish enteritis includes, but is not limited to, viral enteritis, bacterial enteritis, and/or mechanically damaged enteritis.

In one embodiment, bacterial enteritis in grass carp is preferred.

In a second aspect, the invention provides a preparation of a traditional Chinese medicine composition for treating fish enteritis, which is prepared from the following traditional Chinese medicinal materials, by weight, 20-30 parts of common andrographis herb, 20-30 parts of Chinese mahonia, 15-25 parts of humifuse euphorbia herb, 15-25 parts of giant knotweed, 15-25 parts of baical skullcap root, 10-20 parts of astragalus and 10-20 parts of tree peony bark.

Further, the pharmaceutical preparation comprises a pharmaceutical composition for treating fish enteritis and pharmaceutically acceptable auxiliary materials.

Further, the preparation of the traditional Chinese medicine composition can be a paste, a pill, a pellet, a decoction, a granule, a capsule, a powder, a tablet and the like.

In a third aspect, the invention provides an application of the traditional Chinese medicine composition in preparing a medicine for treating fish enteritis.

The medicine properties of the traditional Chinese medicines in the invention are as follows:

andrographis paniculata: bitter taste, cold property, lung meridian, large intestine meridian and bladder meridian entered, and has the effects of clearing heat and detoxicating, cooling blood and detumescence. Has strong capability of clearing away heat and drying dampness, and can be used for treating symptoms caused by exogenous wind-heat; pharmacological studies show that andrographis paniculata contains diterpenoid lactones, flavonoids, polyphenols and the like, wherein the diterpenoid lactones have the highest content and mainly comprise andrographolide, dehydroandrographolide, deoxyandrographolide and the like.

Mahonia fortunei (Mahonia): bitter in flavor, slightly cold in nature, enters liver, stomach and large intestine meridians. Has effects in clearing away heat, eliminating dampness, removing toxic materials, purging pathogenic fire, eliminating dampness, nourishing yin, clearing lung-heat, tonifying deficiency, eliminating phlegm, and relieving cough; can be used for treating hypertension, uremia, heart disease, damp-heat diarrhea, icterohepatitis, conjunctival congestion, insomnia, cough, common cold, fever, bronchitis, acute and chronic tracheitis, and gastritis; pharmacological studies show that the active ingredient of the Chinese mahonia is alkaloid, and has wide pharmacological activity, such as antibacterial, anti-inflammatory, antiviral, anticancer, antiarrhythmic, hypoglycemic and other effects.

Herba Euphorbiae Humifusae: the traditional Chinese medicine composition has the effects of clearing heat and expelling toxin, cooling blood and stopping bleeding, promoting blood circulation and removing blood stasis, promoting diuresis and removing jaundice, promoting lactation and the like, and is mainly used for treating bacillary dysentery, enteritis, traumatic pain, carbuncle and furuncle, bleeding caused by trauma, hematemesis, hemoptysis, increased menstrual flow, hematochezia, jaundice caused by damp-heat, unsmooth milk, bite injury caused by venomous snake and the like clinically at present; pharmacological researches show that the humifuse euphorbia herb contains flavone, sterol, tannin and other components, takes flavone and organic acid substances as main components, takes quercetin as a marking component, has the functions of clearing heat and detoxicating, cooling blood and stopping bleeding, and is mainly used for treating symptoms such as diarrhea, hemoptysis, haematuria, hematochezia, metrorrhagia, sore furuncle, carbuncle and the like.

Giant knotweed rhizome has slightly bitter taste and slightly cold nature, enters liver, gall and lung channels, and has the effects of promoting bile flow, removing jaundice, clearing heat, detoxicating, removing stasis, relieving pain, relieving cough and reducing sputum. Mainly treats the urine stranguria, the intra-abdominal protrusion and the agglomeration are hard like stones and the pain like thorns; pharmacological studies show that giant knotweed contains various active ingredients, mainly emodin, physcion, chrysophanol, polydatin, protocatechuic acid, polysaccharide, amino acid and the like, and the emodin and the polydatin are often used as quality control substances.

Radix Scutellariae Baicalensis: bitter and cold taste, and can be used for reducing the hot air in the organism by entering lung channel, gallbladder channel, spleen channel, large intestine channel and small intestine channel, and has the effects of clearing heat, eliminating dampness, purging pathogenic fire, removing toxic substances, etc.; pharmacological studies show that the main active ingredients of the radix scutellariae are flavonoid compounds, and the flavonoid compounds contain baicalin, baicalein, wogonin, baicalein, beta-sitosterol and the like, and the content of the baicalin is highest.

Radix astragali: slightly warm nature, sweet taste, spleen and lung meridian entered, and has the effects of protecting and consolidating superficial resistance, inducing diuresis to alleviate edema, expelling toxin, promoting granulation, etc. Can be used for treating spontaneous perspiration, night sweat, blood arthralgia, edema, carbuncle, ulcer, and chronic ulcer; pharmacological studies show that the main components of astragalus comprise polysaccharides, saponins, flavonoids and amino groups. The polysaccharide is a main biochemical active component of astragalus and one of main pharmacodynamic components thereof, and related experimental researches prove that the substance can promote humoral immunity and cellular immunity.

Cortex moutan: bitter and pungent taste, slight cold nature, heart, liver and kidney meridian return, has the effects of clearing heat and cooling blood, and activating blood and dissolving stasis, and is mainly used for treating heat entering nutrient blood, epidemic febrile disease, hematemesis and epistaxis, night fever and early cooling, no sweat and bone steaming, amenorrhea and dysmenorrhea, traumatic injury, carbuncle and swelling and sore; pharmacological studies show that the cortex moutan belongs to a stomach-invigorating medicine and has the function of preventing intestinal bleeding.

In the invention, thousands of traditional Chinese medicines are subjected to in-vitro antibacterial screening by utilizing grass carp enteritis pathogenic bacteria, and in-vivo experimental verification is carried out by utilizing a grass carp enteritis model, so that single traditional Chinese medicine with a certain efficacy is screened, and 7 traditional Chinese medicines with better effects are determined by a t-value orthogonal method. And determining the proportion of each traditional Chinese medicine by a uniform design method, and finally obtaining a safe and effective traditional Chinese medicine formula for treating fish enteritis. The traditional Chinese medicine formula is prepared from common andrographis herb, chinese mahonia herb, humifuse euphorbia herb, giant knotweed rhizome, baical skullcap root, membranous milkvetch root and tree peony bark, wherein common andrographis herb has the functions of clearing heat and detoxicating, cooling blood and detumescence; mahonia flower, caulis Mahoniae, and Mahonia flower have effects of clearing heat and eliminating dampness, purging pathogenic fire and removing steaming; herba Euphorbiae Humifusae has effects of clearing heat and removing toxic substances, cooling blood and stopping bleeding, and promoting blood circulation and removing blood stasis; giant knotweed rhizome has the effects of clearing heat and cooling blood, and removing stasis and relieving pain; radix Scutellariae has effects of clearing heat, eliminating dampness, purging pathogenic fire and removing toxic substances; astragalus root, radix astragali benefits the defensive and exterior securing, and expelling toxin and pus; cortex moutan has effects of clearing heat, cooling blood, promoting blood circulation, and removing blood stasis. The seven-ingredient compatibility formula has the effects of clearing heat, drying dampness, detoxifying and stopping dysentery.

The traditional Chinese medicine composition disclosed by the invention takes the effects of clearing heat and drying dampness, and detoxifying and stopping dysentery as treatment rules.

Detailed Description

The present invention will be described in further detail with reference to specific examples, but is not limited thereto. It will be appreciated that modifications and variations will occur to those skilled in the art upon reference to the specification and that such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.

EXAMPLE 1 Chinese medicinal composition

The Chinese medicinal composition is shown in the following table:

table 1 shows various Chinese medicinal compositions

The preparation method comprises the following steps: decocting the above Chinese medicinal materials with 10 times of water for 3 times, each time for 1.5 hr, filtering, mixing filtrates, concentrating to 0.8g crude drug per 1ml, adding ethanol 50ml and propylene glycol 100ml, mixing, refrigerating for 24 hr, filtering, adjusting filtrate with water, and packaging.

Example 2 evaluation of the therapeutic Effect of each of the Chinese medicinal compositions on grass carp enteritis in grass carp enteritis model

1. Purpose of test

And (3) utilizing enteritis pathogenic bacteria to attack toxin, constructing a grass carp enteritis model, and evaluating the treatment effect of each traditional Chinese medicine composition on grass carp enteritis after feeding and administration.

Case characterization of grass carp bacterial enteritis: the diseased fish suffers from anorexia or no ingestion, black body color and slow swimming, and the early dissection can see the congestion and inflammation of the intestinal wall, the thinning of the intestinal wall and the reduction of elasticity; when the internal mucous membrane is seriously fallen off, the intestinal canal has no physical object, the anus is red, the abdomen is lightly pressed, yellow mucous flows out from the anus, and the symptom of purulent white feces or fecal dragging occurs.

2. Test method

2.1 test drug:

each Chinese medicinal composition (see table 1), rhizoma Atractylodis rhizoma Coptidis powder, radix aucklandiae, and rhizoma Atractylodis, and herba Euphorbiae Humifusae powder (herba Euphorbiae Humifusae, radix et rhizoma Rhei, and radix Scutellariae).

Coptis root: cold nature, bitter taste, and heart, spleen, stomach, gallbladder and large intestine channels, has effects of clearing heat, eliminating dampness, purging pathogenic fire, removing toxic substances, and has effects of treating excessive heart fire, diminishing inflammation, relieving pain, and protecting gastric mucosa.

Radix aucklandiae: pungent and bitter in flavor and warm in nature. It enters spleen, stomach, large intestine, triple energizer and gallbladder meridians. Radix aucklandiae has effects of activating qi-flowing, relieving pain, invigorating spleen, and resolving food stagnation. Can be used for treating chest and hypochondrium pain, abdominal distention, diarrhea, dyspepsia, anorexia.

Rhizoma atractylodis: pungent and bitter in flavor, warm in nature, enter spleen and stomach. Has effects of eliminating dampness, invigorating spleen, dispelling pathogenic wind and dispelling cold, and can be used for treating damp obstruction of middle energizer, rheumatalgia, wind cold and dampness exterior syndrome, night blindness and dim eyesight.

Rhubarb: cold in nature, enters spleen and stomach meridians, large intestine meridians, liver meridians and pericardium meridians, and has the effects of cooling blood, removing toxic substances, promoting blood circulation, removing blood stasis, promoting bile flow and removing jaundice.

2.2 test strains:

aeromonas hydrophila (Aeromonas hydrophila, ah) for the test was derived from aquatic animal pathogen library of Shanghai university, strain number: AH10.

2.3 test groups

After temporarily raising grass carp for one week, selecting grass carp with consistent specification, strong physique, active feeding, no disease and no injury for test, randomly dividing 540 grass carp into 19 groups of 3 parallel test cylinders of 10 grass carp per cylinder. Is respectively a blank control group, a toxicity attack control group, a rhizoma atractylodis and rhizoma coptidis powder group the group of the double-yellow humifuse euphorbia herb powder and the experimental group 1-22 groups. Before administration, the test grass carp of each group is detoxified (artificially infected by filling Aeromonas hydrophila bacterial liquid into anus, and bacteria)The concentration of the liquid is 1 multiplied by 10 ⁷ cfu/mL, injection dose of 0.3 mL/tail), the blank group was anal-perfused with an equal amount of sterile physiological saline, 1 time.

2.4 feeding management

The test was carried out in 150L aquariums of the same specification, and each of the Chinese medicinal compositions was added at a feed dosage of 2ml/kg to prepare a medicated bait. Each group is fed with the corresponding bait at a daily feeding rate of 1%, and is fed once every day at 9:00 and 16:00 respectively for 7 days continuously.

2.5 observations index

After 7 days of administration, the grass carp in each jar is fished for body surface and section inspection observation, and is scored for enteritis Disease Activity Index (DAI) (reference DB43T 700-2012, grass carp enteritis disease quarantine technical procedure, song Xuehong, aeromonas hydrophila-induced grass carp intestinal inflammation mechanism study, and Disease Activity Index (DAI) scoring standard of grass carp enteritis is formulated, see table 2 below), and average enteritis Disease Activity Index (DAI) score of the grass carp in each jar is calculated.

TABLE 2 Disease Activity Index (DAI) scoring criteria for grass carp enteritis

3. Experimental results and analysis

The DAI scores of each group are shown in Table 3, the enteritis disease activity index of the grass carp in the blank control group is 0, and the enteritis disease activity index of the grass carp in the attack control group is obviously higher than that of the blank control group (P < 0.05) and is as high as 6.20. The enteritis disease activity index of grass carp in each administration group is significantly higher than that of a blank control group (P < 0.05), but is lower than that of an attack control group (P < 0.05). This result shows that the disease condition of grass carp is improved in each administration group, wherein the sixteen prescription of the traditional Chinese medicine composition has the best effect of only 1.80. According to the result, the optimal composition with priority is determined to be a traditional Chinese medicine composition sixteen, namely 25 parts of common andrographis herb, 25 parts of Chinese mahonia, 20 parts of humifuse euphorbia herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 10 parts of astragalus and 10 parts of tree peony bark.

TABLE 3 DAI scoring results for groups

4. Conclusion of the test

The medicine of the embodiment is added into feed according to recommended dosage, can obviously reduce enteritis symptoms caused by enteritis pathogenic bacteria infection, and has the effect of preventing and treating enteritis. Wherein the Chinese medicinal composition sixteen has the lowest intestinal DAI score of only 1.80, and is recommended to be a preferable optimal formula.

Example 3 test of the efficacy of the Chinese medicinal composition in verifying the extended clinical application of grass carp enteritis

In order to verify the clinical use effect of the traditional Chinese medicine composition, a great number of clinical tests are carried out on the preferred traditional Chinese medicine compositions, and a part of cases are selected and briefly described below.

Case 1

The test was performed on a sixteen traditional Chinese medicine composition fed by mixing baits in a disease-causing pond, and the traditional Chinese medicine composition is provided by Beijing raw Taier technology Co.

Test site: the cultivation area of a certain Beijing cultivation company is 80 mu, and the water depth is 1.8 meters.

Fish body disorder: the ingestion of grass carp is reduced, sporadic death occurs, the grass carp on the edge is fished for cutting, the red swelling of anus and serious ascites can be found, the intestinal tract is reddish, the death condition occurs, and the enteritis of the grass carp caused by aeromonas hydrophila infection is determined through separation and identification.

Treatment protocol: reducing feeding, adding tested Chinese medicinal composition into bait, and adding 200g per 100kg feed

The tested products are used for 1 day and 1 time, 7 days are used continuously, the fish with enteritis symptoms is greatly reduced by sampling anatomical observation on the 5 th day, the fish with enteritis symptoms is not found by sampling anatomical observation on the seventh day, the fish is basically healed, the feeding amount is gradually recovered, and the enteritis symptoms are not recrudesced.

Case 2

The test feeds seventeen traditional Chinese medicine compositions in a disease-causing pond, which is provided by Beijing raw Taier technology Co.

Test site: some fishing ground in Hubei xiantao city has a cultivation area of 200 mu and a water depth of 2.0 meters.

Fish body disorder: the grass carp has poor appetite, dead grass carp is recently found, and a large amount of mucus in the intestinal tract is observed through sampling, so that the grass carp has redness. Congestion, ulceration of the intestinal wall, and greatly reduced ingestion. Is identified as enteritis pathogenic bacteria infection.

Treatment protocol: according to the method, 200g of test product is added into 100kg of feed, and the test product is used for 7 days, the test is carried out on the 4 th day, the whole enteritis symptom is obviously relieved, the test is carried out on the 7 th day, the enteritis symptom is basically disappeared, and the vitality and ingestion are enhanced.

Case 3

The experiment was carried out by feeding eighteen traditional Chinese medicine compositions in a disease-causing pond, and was provided by Beijing raw Taier technology Co.

Test site: the cultivation area of Tianjin certain cultivation company is 65 mu, and the water depth is 1.8 meters.

Fish body disorder: the diseased fish has the disadvantages of decreased appetite, red and swollen anus and enlarged abdomen, and only the second half of the intestinal tract has food through anatomical observation, the intestinal tract is reddened, and more than 200 intestinal tracts die each day. The separated pathogenic bacteria are identified as enteritis caused by aeromonas hydrophila infection.

Treatment protocol: according to the method, 200g of test products are added into 100kg of feed, the death condition is reduced from one hundred of the original feed to more than ten of the current feed after the feed is continuously used for 7 days, the feeding condition is obviously improved, the grass carp is completely recovered from health after the sample taking and the section inspection, and the diseased fish with enteritis symptoms is not found.

Case 4

The nineteen traditional Chinese medicine composition products are fed by mixing baits in a disease-causing pond, and are provided by Beijing raw Taier technology Co.

Test site: a certain culture company in Shanghai has a culture area of 150 mu and a water depth of 2.1 meters.

Fish body disorder: the long-term sporadic death, the recent death number is increased, and the sampling section inspection finds that a large amount of yellow mucus exists in the abdomen, the intestinal congestion and the intestinal wall are light, easy to break, and the ingestion is greatly reduced. Is identified as enteritis pathogenic bacteria infection.

Treatment protocol: 200g of the tested product is added into 100kg of feed, the test is carried out on 7 days, the test is carried out on 5 th days, the enteritis symptoms are obviously relieved, the intestinal canal is full, the test is carried out on 7 th days, the enteritis symptoms are basically disappeared, the vitality of the sick fish is enhanced, and the ingestion is vigorous.

Case 5

The experiment was performed on twenty products of the traditional Chinese medicine composition fed by mixing baits in a disease-causing pond, and the twenty products are provided by Beijing raw Taier technology Co.

Test site: the cultivation area of Jiangsu certain cultivation company is 50 mu, and the water depth is 1.5 meters.

Fish body disorder: the grass carp is decreased in appetite, intestinal congestion is severe in sampling and sectioning, food is not contained in the intestines, the swimming bladder wall is in punctiform congestion, the abdominal cavity is not in yellow mucus, a large number of red blood cells and flaky and exfoliated epithelial-like cells are found in taking intestinal mucus for microscopic examination, and intestinal epithelium is peeled off from the lamina propria. Is identified as viral enteritis.

Treatment protocol: according to the test product of 200g added per 100kg of feed, the test product is continuously used for 7 days, the symptoms of intestinal congestion and bleeding are reduced, the test product is tested on the 7 th day, enteritis is basically recovered to be normal, and vitality and ingestion are normal.

According to a large number of clinical tests, the traditional Chinese medicine composition has a good treatment effect on grass carp enteritis after being continuously used for 7 days, enteritis symptoms can be obviously improved and even healed, and the traditional Chinese medicine composition can be recovered to be normal in a short time, and has a good effect.

In conclusion, the product has ideal effect in preventing and treating enteritis of grass carp, can effectively solve the trouble of aquaculture enterprises and individual households, and reduces the loss of aquaculture fishery.

Claims

1. A traditional Chinese medicine composition for treating fish enteritis is prepared from the following traditional Chinese medicinal materials, by weight, 20-30 parts of common andrographis herb, 20-30 parts of Chinese mahonia, 15-25 parts of humifuse euphorbia herb, 15-25 parts of giant knotweed, 15-25 parts of baical skullcap root, 10-20 parts of astragalus mongholicus and 10-20 parts of cortex moutan.

2. The traditional Chinese medicine composition for treating fish enteritis according to claim 1, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials, by weight, 20-25 parts of common andrographis herb, 20-25 parts of Chinese mahonia, 15-20 parts of humifuse euphorbia herb, 15-20 parts of giant knotweed, 15-20 parts of baical skullcap root, 10-15 parts of astragalus mongholicus and 10-15 parts of cortex moutan.

3. The traditional Chinese medicine composition for treating fish enteritis according to claim 1, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials in parts by weight, 25 parts of common andrographis herb, 25 parts of Chinese mahonia, 20 parts of humifuse euphorbia herb, 15 parts of giant knotweed, 15 parts of baical skullcap root, 10 parts of astragalus mongholicus and 10 parts of cortex moutan.

4. The traditional Chinese medicine composition for treating enteritis of fish according to claim 1, wherein the fish is selected from grass carp, black carp, crucian carp and carp.

5. The traditional Chinese medicine composition for treating fish enteritis according to claim 1, wherein the fish enteritis is selected from one or more of viral enteritis, bacterial enteritis and mechanical injury enteritis.

6. The traditional Chinese medicine composition for treating fish enteritis according to claim 1, wherein the fish enteritis is bacterial enteritis.

7. A preparation of a traditional Chinese medicine composition according to any one of claims 1-3, which is prepared from the following traditional Chinese medicinal materials, by weight, 20-30 parts of common andrographis herb, 20-30 parts of Chinese mahonia, 15-25 parts of humifuse euphorbia herb, 15-25 parts of giant knotweed, 15-25 parts of baical skullcap root, 10-20 parts of astragalus root and 10-20 parts of tree peony bark.

8. The formulation of a traditional Chinese medicine composition according to claim 7, wherein the pharmaceutical formulation comprises a pharmaceutical composition for treating enteritis in fish and pharmaceutically acceptable excipients.

9. The preparation of the traditional Chinese medicine composition according to claim 7, wherein the preparation of the traditional Chinese medicine composition can be ointment, pill, decoction, granule, capsule, powder, tablet and the like.

10. Use of a Chinese medicinal composition according to any one of claims 1-3 in the manufacture of a medicament for the treatment of fish enteritis.