CN116491599A - Composite microbial agent and application thereof in pig feed - Google Patents

Composite microbial agent and application thereof in pig feed Download PDF

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Publication number
CN116491599A
CN116491599A CN202310758619.8A CN202310758619A CN116491599A CN 116491599 A CN116491599 A CN 116491599A CN 202310758619 A CN202310758619 A CN 202310758619A CN 116491599 A CN116491599 A CN 116491599A
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liquid
composite microbial
stirring
sodium alginate
mixed
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王渊
唐光富
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Yunnan Datianong Taibiao Agricultural Technology Co ltd
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Yunnan Datianong Taibiao Agricultural Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention relates to a compound microbial inoculum and application thereof in pig feed, which can obviously inhibit the growth of salmonella and improve the growth performance of feed pigs by adding lactobacillus, saccharomycetes, bifidobacterium, nicotinic acid and soybean isoflavone into the pig feed, and belongs to the technical field of pig feed. The preparation method of the composite microbial inoculum comprises the following steps: 1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution; 2) Centrifuging the activated probiotic liquid, removing supernatant, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A; 3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B; 4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging and cleaning to obtain the composite microbial inoculum.

Description

Composite microbial agent and application thereof in pig feed
Technical Field
The invention belongs to the technical field of pig feeds, and relates to a composite microbial inoculum and application thereof in pig feeds.
Background
Pig feed is the first link of the food safety chain from farm to dining table, and contamination of pig feed with pathogenic bacteria can cause human food-borne diseases. The way of polluting the feed by pathogenic bacteria is different, and salmonella in the pig feed mainly has two sources: firstly, the raw material itself carries salmonella, and secondly, is exogenous. Salmonella is a pathogen that seriously threatens the life health of humans and animals, and swine feed contaminated with salmonella is often the source of infection for humans and animals.
The probiotics preparation can be used as a pig feed additive, can safely enter digestive organs such as pig intestinal tracts and the like through an animal feeding mode, and provides a healthy internal environment for the growth and development of organisms. However, the existing probiotics preparation is only used for promoting the raised pigs to improve the meat quality, flavor and taste, or promoting the rapid growth or skeleton enlargement of the raised pigs, and the pig feed formula is not improved against salmonella.
Disclosure of Invention
The invention aims to provide a composite microbial inoculum and application thereof in pig feed, which can obviously inhibit the growth of salmonella and improve the growth performance of feed pigs by adding lactobacillus, saccharomycetes, bifidobacteria, nicotinic acid and soybean isoflavone.
The aim of the invention can be achieved by the following technical scheme:
the preparation method of the composite microbial inoculum comprises the following steps:
1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution;
2) Centrifuging the activated probiotic liquid and removing the supernatant to obtain bacterial mud, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A;
3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B;
4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging and cleaning to obtain the composite microbial inoculum.
From the scheme, lactobacillus and bifidobacterium can inhibit the growth of salmonella through mechanisms such as competitive displacement; the nicotinic acid has an adjusting effect on the intestinal flora of pigs, so that the number of salmonella in the intestinal tract is reduced; soy isoflavones are able to inhibit salmonella growth in the pig gut by binding to estrogen receptors.
As a preferable technical scheme of the invention, in the step 1), the ultrasonic dispersion time is 20-30min, and the mass concentration of the sodium alginate solution is 3-5%.
As a preferable technical scheme of the invention, in the step 2), the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 30-40min at a rotation speed of 300-500 r/min; the stirring condition is that stirring is carried out for 40-60min at the rotating speed of 600-800 r/min.
As a preferred technical scheme of the invention, in the step 2), the probiotic liquid is obtained by inoculating lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid on a mixed fermentation liquid culture medium for culture, wherein the inoculation ratio of the lactobacillus strain culture liquid, the saccharomycete strain culture liquid and the bifidobacterium strain culture liquid is 1:2-5:3-8; the lactobacillus strain culture solution is inoculated to the mixed fermentation liquid culture medium in an inoculum size of 1-3 percent, wherein the proportions are all volume ratios.
As a preferable technical scheme of the invention, in the step 3), the mass ratio of the calcium chloride to the hydroxyl chitosan to the deionized water is 30-50:5-8:55-78; the ultrasonic dispersion time is 10-20min.
As a preferred technical scheme of the invention, in the steps 2) -3), the mass ratio of the probiotic liquid to the sodium alginate solution to the nicotinic acid to the soybean isoflavone to the mixed liquid B is 2-4:8-15:0.1-0.2:0.2-0.4:3-4.
As a preferable technical scheme of the invention, in the step 4), the low-temperature mixing condition is that the treatment is carried out for 24 hours at the temperature of 10-20 ℃, and the washing condition is that the washing is carried out by using normal saline.
The composite microbial inoculum prepared by the scheme of the invention can be applied to pig feed.
The invention discloses application of a composite microbial inoculum in pig feed, wherein the mass ratio of the composite microbial inoculum to the pig feed is 0.2-0.5:100.
the invention has the beneficial effects that:
according to the scheme, the pig feed containing lactobacillus, saccharomycetes, bifidobacteria, nicotinic acid and soybean isoflavone can remarkably inhibit the growth of salmonella, and meanwhile, the growth performance of feed pigs is improved; lactobacillus can inhibit salmonella growth by competitive exclusion and production of antibacterial substances. The saccharomycete can promote pig intestinal microorganism balance and strengthen immunity, so as to reduce the risk of salmonella infection. The bifidobacterium can promote nutrient absorption and digestion and improve the growth performance of pigs. Nicotinic acid and soy isoflavone help to improve the immunity and digestion and absorption of pigs.
Detailed Description
In order to further describe the technical means and effects adopted by the present invention for achieving the intended purpose, the following detailed description is given below with reference to the embodiments, structures, features and effects according to the present invention.
Example 1
The preparation method of the composite microbial inoculum comprises the following steps:
1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution; wherein the ultrasonic dispersion time is 20min, and the mass concentration of the sodium alginate solution is 3%;
2) Centrifuging the activated probiotic liquid and removing the supernatant to obtain bacterial mud, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A; wherein the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 30min at a rotation speed of 300 r/min; the stirring condition is that stirring is carried out for 40min at the rotating speed of 600 r/min;
the probiotic liquid is obtained by inoculating lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid on a mixed fermentation liquid culture medium for culture, wherein the inoculation proportion of the lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid is 1:2:3, a step of; inoculating lactobacillus strain culture solution into the mixed fermentation liquid culture medium with an inoculum size of 1%;
3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B; wherein, the mass ratio of the calcium chloride to the hydroxyl chitosan to the deionized water is 30:5:55; the ultrasonic dispersion time is 10min; wherein, the mass ratio of the probiotic liquid to the sodium alginate solution to the nicotinic acid to the soybean isoflavone to the mixed liquid B is 2:8:0.1:0.2:3, a step of;
4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging, and cleaning to obtain a composite microbial inoculum; wherein the low-temperature mixing condition is that the treatment is carried out for 24 hours at the temperature of 10 ℃, and the cleaning condition is that the washing is carried out by adopting normal saline.
The mass ratio of the composite microbial inoculum to the pig feed is 0.2:100.
example 2
The preparation method of the composite microbial inoculum comprises the following steps:
1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution; wherein the ultrasonic dispersion time is 23min, and the mass concentration of the sodium alginate solution is 3.5%;
2) Centrifuging the activated probiotic liquid and removing the supernatant to obtain bacterial mud, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A; wherein the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 33min at a rotation speed of 380 r/min; the stirring condition is that stirring is carried out for 46min at the rotating speed of 680 r/min;
the probiotic liquid is obtained by inoculating lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid on a mixed fermentation liquid culture medium for culture, wherein the inoculation proportion of the lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid is 1:3:4.2; inoculating lactobacillus strain culture solution into the mixed fermentation liquid culture medium with an inoculum size of 1.3%;
3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B; wherein, the mass ratio of the calcium chloride to the hydroxyl chitosan to the deionized water is 36:6.2:60; the ultrasonic dispersion time is 13min; wherein, the mass ratio of the probiotic liquid to the sodium alginate solution to the nicotinic acid to the soybean isoflavone to the mixed liquid B is 2.8:12:0.13:0.28:3.3;
4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging, and cleaning to obtain a composite microbial inoculum; wherein the low-temperature mixing condition is that the treatment is carried out for 24 hours at the temperature of 13 ℃, and the cleaning condition is that the washing is carried out by adopting normal saline.
The mass ratio of the composite microbial inoculum to the pig feed is 0.3:100.
example 3
The preparation method of the composite microbial inoculum comprises the following steps:
1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution; wherein the ultrasonic dispersion time is 25min, and the mass concentration of the sodium alginate solution is 4%;
2) Centrifuging the activated probiotic liquid and removing the supernatant to obtain bacterial mud, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A; wherein the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 38min at a rotation speed of 400 r/min; the stirring condition is that stirring is carried out for 52min at the rotating speed of 720 r/min;
the probiotic liquid is obtained by inoculating lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid on a mixed fermentation liquid culture medium for culture, wherein the inoculation proportion of the lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid is 1:4:6, preparing a base material; inoculating lactobacillus strain culture solution into mixed fermentation liquid culture medium with inoculum size of 2.2%;
3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B; wherein, the mass ratio of the calcium chloride to the hydroxyl chitosan to the deionized water is 45:7:72; the ultrasonic dispersion time is 18min; wherein, the mass ratio of the probiotic liquid to the sodium alginate solution to the nicotinic acid to the soybean isoflavone to the mixed liquid B is 3.5:14:0.16:0.34:3.8;
4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging, and cleaning to obtain a composite microbial inoculum; wherein the low-temperature mixing condition is that the treatment is carried out for 24 hours at the temperature of 18 ℃, and the cleaning condition is that the washing is carried out by adopting normal saline.
The mass ratio of the composite microbial inoculum to the pig feed is 0.4:100.
example 4
The preparation method of the composite microbial inoculum comprises the following steps:
1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution; wherein the ultrasonic dispersion time is 30min, and the mass concentration of the sodium alginate solution is 5%;
2) Centrifuging the activated probiotic liquid and removing the supernatant to obtain bacterial mud, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A; wherein the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 40min at a rotation speed of 500 r/min; the stirring condition is that stirring is carried out for 60min at the rotating speed of 800 r/min;
the probiotic liquid is obtained by inoculating lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid on a mixed fermentation liquid culture medium for culture, wherein the inoculation proportion of the lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid is 1:5:8, 8; inoculating lactobacillus strain culture solution into the mixed fermentation liquid culture medium with an inoculum size of 3%;
3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B; wherein, the mass ratio of the calcium chloride to the hydroxyl chitosan to the deionized water is 30-50:5-8:78; the ultrasonic dispersion time is 20min; wherein, the mass ratio of the probiotic liquid to the sodium alginate solution to the nicotinic acid to the soybean isoflavone to the mixed liquid B is 4:15:0.2:0.4:4, a step of;
4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging, and cleaning to obtain a composite microbial inoculum; wherein the low-temperature mixing condition is that the treatment is carried out for 24 hours at the temperature of 20 ℃, and the cleaning condition is that the washing is carried out by adopting normal saline.
The mass ratio of the composite microbial inoculum to the pig feed is 0.5:100.
comparative example 1
Step 2), taking activated probiotic liquid, centrifuging and removing supernatant to obtain bacterial mud, adding prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding soybean isoflavone and stirring uniformly to obtain mixed liquid A; wherein the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 38min at a rotation speed of 400 r/min; the stirring condition is that stirring is carried out for 52min at the rotating speed of 720 r/min;
in comparison with example 3, the difference is that comparative example 1 does not use nicotinic acid, and the remaining components, preparation steps and parameters are identical.
Comparative example 2
Step 2), taking activated probiotic liquid, centrifuging and removing supernatant to obtain bacterial mud, adding prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and stirring uniformly to obtain mixed liquid A; wherein the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 38min at a rotation speed of 400 r/min; the stirring condition is that stirring is carried out for 52min at the rotating speed of 720 r/min;
in comparison with example 3, comparative example 2 was different in that soy isoflavone was not used, and the remaining components, preparation steps and parameters were identical.
Comparative examples 3 to 5
The difference from example 3 is that the inoculation ratios of the lactobacillus species culture solution, the yeast species culture solution and the bifidobacterium species culture solution of comparative examples 3 to 5 are shown in Table 1, and the remaining components, the preparation steps and the parameters are the same.
TABLE 1
Comparative example 6
In comparison with example 3, comparative example 6 does not use hydroxychitosan, and the remaining components, preparation steps and parameters are identical.
The following tests were performed on examples 1-4 and comparative examples 1-6:
the 60 piglets were randomly divided into 10 groups of 6 piglets, and each group was fed with the feeds prepared in examples 1-4 and comparative examples 1-6, respectively, with a test period of 100d.
Growth performance test
The pigs were weighed on empty stomach in each repeat basis on day 07:00 of start and end of test (12 h of cut before weighing). After the test, the feed intake of each repetition of the control group and the test group was counted, and the average daily gain and the number of piglets per weight ratio were calculated, and the obtained results are shown in Table 2.
Bacteriostasis test
10 parts of a certain pig feed was taken, 1kg of each part was weighed, and 0.4wt% of the pig feed additives obtained in examples 1 to 4 and comparative examples 1 to 6 were added, respectively, and 1.004kg of the same pig feed was taken as a control group.
The number of viable bacteria was counted by taking 30 culture dishes each having salmonella grown thereon. The 30 dishes were divided into 10 groups, and equal amounts of the complex microbial agents of examples 1 to 4 and comparative examples 1 to 6 and the pig feed of the control group were added to the 10 groups, respectively. After 15 days, the number of viable bacteria in the culture dish was counted again, and the antibacterial rate was calculated, and the results are shown in table 2.
TABLE 2
As can be seen from the test results in Table 2, the compound bacteria prepared in examples 1-4 are added into pig feed, compared with comparative examples 1-6, and the inhibition effect on the growth of raised pigs and salmonella is significantly better than that of comparative examples 1-6; according to the scheme, the pig feed containing lactobacillus, saccharomycetes, bifidobacteria, nicotinic acid and soybean isoflavone can remarkably inhibit the growth of salmonella, and meanwhile, the growth performance of feed pigs is improved; lactobacillus can inhibit salmonella growth by competitive exclusion and production of antibacterial substances. The saccharomycete can promote pig intestinal microorganism balance and strengthen immunity, so as to reduce the risk of salmonella infection. The bifidobacterium can promote nutrient absorption and digestion and improve the growth performance of pigs. Nicotinic acid and soy isoflavone help to improve the immunity and digestion and absorption of pigs.
The present invention is not limited to the above embodiments, but is capable of modification and variation in detail, and other modifications and variations can be made by those skilled in the art without departing from the scope of the present invention.

Claims (9)

1. The preparation method of the composite microbial inoculum is characterized by comprising the following steps of:
1) Adding sodium alginate into deionized water, and performing ultrasonic dispersion to obtain sodium alginate solution;
2) Centrifuging the activated probiotic liquid and removing the supernatant to obtain bacterial mud, adding the prepared sodium alginate solution into the bacterial mud, stirring and mixing uniformly, adding nicotinic acid and soybean isoflavone, and stirring uniformly to obtain a mixed liquid A;
3) Dissolving calcium chloride and hydroxyl chitosan in deionized water, and performing ultrasonic dispersion to obtain a mixed solution B;
4) Slowly dripping the mixed solution A into the mixed solution B, mixing at low temperature, centrifuging and cleaning to obtain the composite microbial inoculum.
2. The composite microbial agent of claim 1, wherein: in the step 1), the ultrasonic dispersion time is 20-30min, and the mass concentration of the sodium alginate solution is 3-5%.
3. The composite microbial agent of claim 1, wherein: in the step 2), the centrifugation condition is centrifugation for 10min at a rotation speed of 5000r/min, and the stirring and mixing condition is stirring for 30-40min at a rotation speed of 300-500 r/min; the stirring condition is that stirring is carried out for 40-60min at the rotating speed of 600-800 r/min.
4. The composite microbial agent of claim 1, wherein: in the step 2), the probiotic liquid is obtained by inoculating lactobacillus strain culture liquid, saccharomycete strain culture liquid and bifidobacterium strain culture liquid on a mixed fermentation liquid culture medium for culture, wherein the inoculation proportion of the lactobacillus strain culture liquid, the saccharomycete strain culture liquid and the bifidobacterium strain culture liquid is 1:2-5:3-8; the lactobacillus strain culture solution is inoculated on the mixed fermentation liquid culture medium with an inoculum size of 1-3 percent.
5. The composite microbial agent of claim 1, wherein: in the step 3), the mass ratio of the calcium chloride to the hydroxyl chitosan to the deionized water is 30-50:5-8:55-78; the ultrasonic dispersion time is 10-20min.
6. The composite microbial agent of claim 1, wherein: in the steps 2) -3), the mass ratio of the probiotic liquid to the sodium alginate solution to the nicotinic acid to the soybean isoflavone to the mixed liquid B is 2-4:8-15:0.1-0.2:0.2-0.4:3-4.
7. The composite microbial agent of claim 1, wherein: in the step 4), the low-temperature mixing condition is that the treatment is carried out for 24 hours at the temperature of 10-20 ℃, and the washing condition is that the washing is carried out by using normal saline.
8. Use of a composite microbial agent according to any one of claims 1 to 7, characterized in that: the composite microbial inoculum is applied to pig feed.
9. The use of a composite microbial agent according to claim 8, wherein: the mass ratio of the composite microbial inoculum to the pig feed is 0.2-0.5:100.
CN202310758619.8A 2023-06-26 2023-06-26 Composite microbial agent and application thereof in pig feed Pending CN116491599A (en)

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