CN103598654B - Pollen active probiotic drink and preparation method thereof - Google Patents

Pollen active probiotic drink and preparation method thereof Download PDF

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CN103598654B
CN103598654B CN201310578456.1A CN201310578456A CN103598654B CN 103598654 B CN103598654 B CN 103598654B CN 201310578456 A CN201310578456 A CN 201310578456A CN 103598654 B CN103598654 B CN 103598654B
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pollen
lactobacillus
liquid
active probiotic
preparation
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CN103598654A (en
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裘纪莹
陈蕾蕾
陈相艳
刘孝永
王未名
张奇志
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Institute of Agro Food Science and Technology of Shandong Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25—Honey; Honey substitutes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)

Abstract

The invention provides a pollen active probiotic drink and a preparation method thereof. The pollen active probiotic drink is prepared by the preparation method comprising the following steps: with pollen as a raw material, matching with a proportion of malt sugar and/or galactose; mixing and fermenting by two probiotics including lactobacillus BL1 and lactobacillus BL2, and then blending. By adopting the pollen active probiotic drink, two bacterial strains with strong acid resistance and a plurality of excellent characters are preferred aiming at the characteristics that the pollen raw material is strong in acidity and is not easily utilized by microorganisms. The viable counts of probiotics of the pollen active probiotic drink within 30 days can be kept over 10<11> cfu/L by controlling a fermentation technology; pollen flavonoid glycoside can be biologically converted, so as to obtain a lot of flavonoid aglycones, so that the bioavailability of pollen flavone is improved. Meanwhile, the pollen active probiotic drink contains other pollens and probiotic fermented products, so that the pollen active probiotic drink is abundant in nutrition, thick in smell, and palatable in sour and sweet, and simultaneously has the functions of maintaining beauty and keeping young, boosting immunity, reducing cholesterol, regulating intestinal flora, preventing constipation and the like.

Description

A kind of pollen active probiotic drink and preparation method thereof
Technical field
The present invention relates to a kind of pollen active probiotic drink with health care and preparation method thereof, belong to technical field of health care food.
Background technology
Along with the raising of health of people consciousness, health food more and more causes the concern of people.Edible Bee Pollen is with a long history in China, Bee Pollen is rich in nutriment and the health-care components such as flavones, polysaccharide such as the necessary amino acid of human body, organic acid, unrighted acid, nucleic acid, enzyme, mineral element, vitamin, has high nutrition and health care and medical value to human body.Modern study shows, Bee Pollen has anti-oxidant, antifatigue, anti-ageing, multiple efficacies such as raising immunity, Improving memory etc., and the Bee Pollen product on current health-product market is more single, mostly is the form of pulvis, edible palatability is poor, and the bioavailability of plurality of active ingredients is lower.Therefore, the Bee Pollen product of research and development high technology content, significant.
Probio refers to improves host's microecological balance and plays beneficial effect, reach the active bacteria formulation and metabolite thereof that improve host health level and health status, probio is present in each corner tellurian, and bacterium useful in animal body or fungi mainly contain: lactic acid bacteria, Bifidobacterium, saccharomycete etc.Lactic acid bacteria is important probio, and biodiasmin can secrete the material useful to health when growing, and therefore has more benefit to human body: 1, have the effect promoting gastric secretion, improve appetite, strengthen digestion; Probio can by the lactose in milk and breaks down proteins, makes human body more easy to digest and absorb; 2, safeguard the gut flora ecological balance, form biological barrier, suppress harmful bacteria to the invasion of enteron aisle; 3, the generation of some carcinogen can be reduced, thus have protective effect on cancer risk; 4, the breeding of spoilage organisms in enteron aisle can be suppressed, and weaken the toxin that spoilage organisms produces in enteron aisle; Promote that intestines peristalsis and thalline raised growth change osmotic pressure and prevent constipation by producing a large amount of SCFAs; 5, have the effect reducing cholesterol, the people of suitable especially high fat of blood drinks; 6, improve immune function of human body, lactic acid bacteria can produce the material that some strengthen immunologic function, can improve human immunity, prevent disease.In general, no matter be Post operation, or acute, chronic recover after patient, in order to disease therapy or protect from infection and all once taken or injected a large amount of antibiotic, make Intestinal flora that very large change occur, some useful enteron aisle bacterium are suppressed or kill, and cause flora imbalance.Take a certain amount of probiotic lactic acid bacterium every day, can maintain gut flora balance, regulating intestinal canal profitable strain is to normal level.
At present about the report of Bee Pollen fermented product is as follows.Qiao Lin (2005) has carried out the development of Bee Pollen solid state fermentation nutrition fortifier; by saccharomycete, lactic acid bacteria, bafillus natto, single bacterium, mixed culture solid state fermentation test are carried out to pollen; the suitableeest bacterial strain finally determining Bee Pollen solid state fermentation is bafillus natto and lactobacillus acidophilus, and two bacterium mix in 1: 1 ratio; The suitableeest fermentation mode is shallow-layer aerobic fermentation, and vaccination ways is for first to connect bafillus natto, and the 3d that ferments accesses lactobacillus acidophilus again; Inoculum concentration is 10%, and pollen water content is 35 ~ 40%, and fermentation temperature is 30 DEG C, and fermentation period is 8d, has carried out scale-up simultaneously.The fresh perfume (or spice) of pollen smell after fermentation, sweet and sour taste, in pollen, the viable count of bafillus natto is 4.5 × 10 7cfu/g, the viable count of lactobacillus acidophilus is 2.7 × 10 7cfu/g.Crude protein adds 5.9%, and crude fat reduces 1.57%, and free amino acid adds 0.02%, and total organic acids adds 0.79g/100g, and pH value drops to 4.2, and lactic acid recruitment is maximum reaches 0.20g/100g.Yang Wen superfine (2012) has carried out the process optimization of bee bread source lactobacillus-fermented bee pollen form cole, adopts solid state fermentation, lactobacillus inoculum amount 13%, Monascus fermentation broth addition 10%, amount of water 25%, fermentation temperature 33 DEG C.After fermentation, Bee Pollen palatability is improved, and improves nutritive value.Wang Cuihua etc. (2009) have developed a kind of functional yoghourt containing Bee Pollen, and in yogurt production, with the addition of the Bee Pollen of 0.1% ~ 2%, probio can remain on 10 in 21 days 6~ 10 9cfu/ml.Sun Shiyao (2008), old Hunan (2011) also have studied honey pollen wine separately.
But in above-mentioned research, the general inoculum concentration of Bee Pollen solid state fermentation is large, fermentation time is long, inoculum concentration is more than 10%, and fermentation time also wants about 1 week, and solid state fermentation exists the shortcoming of product quality instability simultaneously, easily the bacteria infection by the impact of environment.No matter be Bee Pollen is added to Yoghourt or be used for wine brewing in liquid state fermentation, general Bee Pollen addition is all fewer, and Bee Pollen consumption is generally no more than 2%.We determine the natural pH of Bee Pollen between 4.5 ~ 5.0, and under the pH value that this is lower, microorganism is easily subject to acid the suppression and is difficult to growth and fermentation.May also this reason just, existingly need larger inoculum concentration and long fermentation time when fermenting in solid state fermentation mode, pH declines also obviously little simultaneously.And Bee Pollen consumption can not be too large during liquid state fermentation, otherwise can growth of microorganism be suppressed and not reach ferment effect.So, select the microbial inoculum being applicable to Bee Pollen fermentation to seem extremely important.
Lactobacillus BL1, BL2 are separated from pickles by applicant Agricultural Products Research Institute, Shandong Academy of Agricultural Sciences of patent of the present invention to identify and the two strain acid resistance lactic acid bacteria (Liu Xiaoyong preserved, Qiu Jiying, Sun Xin, Deng. the separation qualification of two strains of lactic acid bacteria and Mixed culture pre-test thereof in pickles. Shandong agricultural sciences ［ J ］, 2012,44 (9): 85-89.).Above-mentioned article is only reported the separation qualification of above-mentioned two strain bacterial strains.But do not report function and the purposes of this bacterial strain.
Summary of the invention
Instant invention overcomes the deficiency existed in above-mentioned Bee Pollen product sweat, provide a kind of pollen active probiotic drink and preparation method thereof.Bee Pollen is through lactobacillus BL1 and lactobacillus BL2 two kinds of strain excellent Mixed culture, and Main Flavonoids glycosides is changed into corresponding aglycon by part biological---and Kaempferol, improves the bioavailability of Bee Pollen flavones.Simultaneously containing other Bee Pollen functional component a large amount of and probiotics fermention product, have anti-oxidant, antifatigue, anti-ageing, improve immunity, norcholesterol and regulate the functions such as stomach and intestine balance.
An object of the present invention is to provide a kind ofly has anti-oxidant, antifatigue, anti-ageing, the pollen active probiotic drink that improves the functions such as immunity, Improving memory, adjustment stomach and intestine balance; Two of object is to provide the preparation method of this pollen active probiotic drink.
An object of the present invention realizes by following technical measures: a kind of pollen active probiotic drink, is characterized in that, is made up of the raw material of following weight parts: Bee Pollen 3 ~ 10, maltose and/or galactolipin 3 ~ 5, water 100, honey element 0.030 ~ 0.065, acesulfame potassium 0.020 ~ 0.030; With Bee Pollen and maltose and/or galactolipin for raw material, add water and make fermentation medium, ferment than mixes liquid through lactobacillus BL1, lactobacillus BL2 two kinds of probio equal-volumes, after-ripening, and add and form with water-soluble honey element and acesulfame potassium allotment.Twice water total amount in front and back is 100 weight portions, wherein makes the water consumption > 50 parts of fermentation medium; The two preferred weight ratio is 99:1.
Two of object of the present invention realizes by following technical measures:
(1) each raw material is got by above-mentioned weight portion;
(2) preparation of fermentation medium: Bee Pollen removal of impurities also pulverized 40 ~ 80 mesh sieves, and 40 mesh sieves pulverized by maltose and/or galactolipin; By Bee Pollen and maltose and/or galactolipin mixing, slowly add the water of 99 weight portions while stirring, to stir and by gauze or 4 layers of filtered through gauze;
(3) ferment: by fermented and cultured based on 115 DEG C of insulations sterilization in 15 ~ 30 minutes, and be cooled to less than 37 DEG C; Add fermentation medium volume 3 ~ 5%(preferably 4 %) mixed bacteria expand liquid, 30 ~ 37 DEG C of sealing and standing are cultivated 18 ~ 30 hours; Treat that zymotic fluid pH is down to 3.10 ~ 3.30, total acid (in lactic acid) reaches 5 ~ 7 g/L, and total plate count reaches 10 11it is fermentation termination during cfu/L;
Wherein mixed bacteria expands liquid is expand liquid with lactobacillus BL1 bacterial classification expansion liquid and lactobacillus BL2 bacterial classification to add by after equal-volume mixing;
(4) after-ripening and seasoning: the product reaching fermentation termination is left standstill in 4 ~ 8 DEG C and carries out after-ripening in 8 ~ 12 hours, be beneficial to the formation of fragrance; Honey element and acesulfame potassium are used in advance the water-soluble solution of 1 weight portion, 95 DEG C of insulations sterilization in 20 minutes, to join in fermented product in sterile working mode and mixes.
(5) packaging and preservation: sterile filling also in 4 ~ 8 DEG C of circulations, storage, shakes up before drinking.30 days shelf-lifves, pH>=3.0 in the shelf-life, total acid (in lactic acid) 5 ~ 9 g/L, total plate count 10 11cfu/L.
The preparation method that described lactobacillus BL1 bacterial classification expands liquid is:
(1) lactobacillus BL1 probio activation: cultivations of rule on MRS solid medium by lactobacillus BL1, is separated single bacterium colony and transfers MRS slant medium, and 37 DEG C of cultivations 24 ~ 72 hours are for subsequent use;
(2) preparation of lactobacillus BL1 liquid spawn: by inclined plane inoculating in liquid MRS culture medium, cultivates 18 ~ 24 hours, obtains liquid spawn for 37 DEG C;
(3) lactobacillus BL1 bacterial classification expands the preparation of liquid: compound concentration is the Bee Pollen aqueous solution of 3 ~ 5wt%, 115 DEG C of sterilizing 15 ~ 20min, are cooled to normal temperature, with the volume ratio inoculation liquid spawn of 3 ~ 5%, 37 DEG C of quiescent culture 1 ~ 2 day, obtain lactobacillus BL1 bacterial classification and expand liquid.
Described lactobacillus BL2 bacterial classification expands the preparation method of preparation method with lactobacillus BL1 bacterial classification expansion liquid of liquid.
The present invention selects lactobacillus BL1 and lactobacillus BL2 two strain excellents, carries out liquid mixed culture fermentation with higher concentration Bee Pollen for primary raw material, has given full play to the effect of two bacterium in Bee Pollen fermentation.The present invention is shown by research, and lactobacillus BL1 and lactobacillus BL2 acid resistance by force, can utilize the Bee Pollen of higher concentration to carry out quick liquid state fermentation.It is fast that lactobacillus BL2 produces acid, but during single bacterium fermentation Bee Pollen after acidifying relatively more serious, storage life is short, and it is slower than lactobacillus BL2 that lactobacillus BL1 produces acid, and both mixed culture fermentations also control zymotechnique, can ensure that in 30 days, probio number remains on 10 11more than cfu/L.Lactobacillus BL1 has very strong flavonoid glycoside conversion capability, the flavonoid glycoside enriched can be converted into corresponding aglycon in Bee Pollen, thus improves human body to the bioavailability of Bee Pollen.Meanwhile, lactobacillus BL1 and lactobacillus BL2 has antibacterial characteristics, all has certain inhibitory action to food-borne pathogens such as salmonella, Escherichia coli, staphylococcus aureus and vibrio parahemolyticus; Two bacterial strains all have the ability of cholesterol degradation, and the external degrading rate of cholesterol of lactobacillus BL1 and lactobacillus BL2 is respectively 38.53% and 32.09%.
On the whole, pollen active probiotic drink compound provided by the invention also enhances the health care of Bee Pollen and probio, by biological conversion and intensification Bee Pollen anti-oxidant, antifatigue, anti-ageing, improve the multiple efficacies such as immunity, Improving memory, there is again the probio number of high-load, stomach and intestine can be regulated to balance, reduce cholesterol, integrate health care and treat, cater to people's prevention, health care, treat the medical science viewpoint combined with rehabilitation, and the shelf-life reaches 30 days, market prospects are more extensive.
Accompanying drawing explanation
Fig. 1 Bee Pollen probiotics fermention process Main Flavonoids glycosides degraded situation.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described, but be not limited thereto.
Embodiment 1:
Lactobacillus BL1 bacterial classification expands the preparation of liquid;
MRS solid medium is: peptone 5 g, beef extract 5 g, yeast extract 2. 5 g, glucose 2. 5 g, Triammonium citrate 1. 0 g, sodium acetate 1. 56 g, potassium acetate 1. 125 g, sodium hydrogen phosphate 0. 815 g, four water manganese sulfate 0. 125 g, epsom salt 0. 29 g, Tween 80 is 0. 5 ml, agar 10 g, distilled water 500 ml, pH6. 5,121 DEG C of sterilizing 20 min.
MRS slant medium is: with MRS solid medium.
Liquid MRS culture medium is: for removing agar in MRS solid medium component.
(1) probio activation: cultivations of rule on MRS solid medium by lactobacillus BL1, is separated single bacterium colony and transfers MRS slant medium, and 37 DEG C of cultivations 48 hours are for subsequent use;
(2) liquid spawn: by inclined plane inoculating in liquid MRS culture medium, cultivates 20 hours, obtains liquid spawn for 37 DEG C;
(3) bacterial classification expands liquid: the Bee Pollen aqueous solution preparing 3% weight, and 115 DEG C of sterilizing 15min, are cooled to normal temperature, and with the volume ratio inoculation liquid spawn of 4%, 37 DEG C of quiescent culture 1 day, obtain lactobacillus BL1 bacterial classification and expand liquid;
The preparation method that described lactobacillus BL2 bacterial classification expands liquid is the same.
Embodiment 2:
(1) preparation of fermentation medium: Bee Pollen removal of impurities also pulverized 40 mesh sieves, and 40 mesh sieves pulverized by maltose and galactolipin.5kg Bee Pollen, 2kg maltose and 2kg galactolipin are mixed, slowly adds the water of 99kg while stirring, to stir and by gauze or 4 layers of filtered through gauze;
(2) ferment: by fermented and cultured based on 115 DEG C of insulations sterilization in 20 minutes, and be cooled to less than 37 DEG C.The mixed bacteria adding 4% volume of fermentation medium expands liquid, and wherein mixed bacteria expands liquid is add by after the volume ratio mixing of 1:1 with the bacterial classification expansion liquid of lactobacillus BL1 and lactobacillus BL2 two probios.37 DEG C of sealing and standing cultivate 24 hours, treat that zymotic fluid pH is down to 3.10 ~ 3.20, and total acid (in lactic acid) reaches 5 ~ 7 g/L, and total plate count reaches 10 11it is fermentation termination during cfu/L;
(3) after-ripening and seasoning: the product reaching fermentation termination is left standstill in 4 DEG C and carries out after-ripening in 12 hours, be beneficial to the formation of fragrance.The acesulfame potassium of the honey element of 0.065kg and 0.030kg is used in advance the water-soluble solution of 1kg, 95 DEG C of insulations sterilization in 20 minutes, to join in fermented product in sterile working mode and mix;
(4) packaging and preserving: sterile filling 4 DEG C of circulations, to store, shakes up before drinking.30 days shelf-lifves, pH≤3.0 in the shelf-life, total acid (in lactic acid) 5 ~ 9 g/L, total plate count 10 11cfu/L.
Bee Pollen probiotics fermention process Main Flavonoids glycosides degraded situation is shown in Fig. 1 and table 1.
As can be seen from Figure 1: the two-O-β-D-Glucose glycosides of the Main Flavonoids glycosides Kaempferol-3,4'-of Bee Pollen and Kaempferol-3-O-β-D-(2-O-β-D-Glucose base in sweat) content of glucopyranoside significantly declines.As can be seen from Table 1: the Kaempferol content of Bee Pollen after 1 day that ferments increases substantially, and increment is 91%.To sum up illustrate, Bee Pollen is through lactobacillus BL1 and lactobacillus BL2 two kinds of strain excellent Mixed culture, and Main Flavonoids glycosides is changed into corresponding aglycon by part biological---and Kaempferol, improves the bioavailability of Bee Pollen flavones.
Embodiment 3
(1) preparation of fermentation medium: Bee Pollen removal of impurities also pulverized 40 mesh sieves, and 40 mesh sieves pulverized by maltose.By 6kg Bee Pollen and the mixing of 4kg maltose, slowly add the water of 99kg while stirring, stir and use 4 layers of filtered through gauze;
(2) ferment: by fermented and cultured based on 115 DEG C of insulations sterilization in 25 minutes, and be cooled to less than 37 DEG C.The mixed bacteria adding 4% volume of fermentation medium expands liquid, and wherein mixed bacteria expands liquid is add by after the volume ratio mixing of 1:1 with the bacterial classification expansion liquid of lactobacillus BL1 and lactobacillus BL2 two probios.37 DEG C of sealing and standing cultivate 24 hours, treat that zymotic fluid pH is down to 3.10 ~ 3.20, and total acid (in lactic acid) reaches 5 ~ 7 g/L, and total plate count reaches 10 11it is fermentation termination during cfu/L;
(3) after-ripening and seasoning: the product reaching fermentation termination is left standstill in 4 DEG C and carries out after-ripening in 12 hours, be beneficial to the formation of fragrance.The acesulfame potassium of the honey element of 0.05kg and 0.025kg is used in advance the water-soluble solution of 1kg, 95 DEG C of insulations sterilization in 25 minutes, to join in fermented product in sterile working mode and mix;
(4) packaging and preserving: sterile filling 4 DEG C of circulations, to store, shakes up before drinking.30 days shelf-lifves, pH≤3.0 in the shelf-life, total acid (in lactic acid) 5 ~ 9 g/L, total plate count 10 11cfu/L.
Embodiment 4
(1) preparation of fermentation medium: Bee Pollen removal of impurities also pulverized 40 mesh sieves, and 40 mesh sieves pulverized by galactolipin.By 5kg Bee Pollen and the mixing of 3kg galactolipin, slowly add the water of 99kg while stirring, stir and filter with gauze;
(2) ferment: by fermented and cultured based on 115 DEG C of insulations sterilization in 20 minutes, and be cooled to less than 37 DEG C.The mixed bacteria adding 4% volume of fermentation medium expands liquid, and wherein mixed bacteria expands liquid is add by after the volume ratio mixing of 1:1 with the bacterial classification expansion liquid of lactobacillus BL1 and lactobacillus BL2 two probios.37 DEG C of sealing and standing cultivate 24 hours, treat that zymotic fluid pH is down to 3.10 ~ 3.20, and total acid (in lactic acid) reaches 5 ~ 7 g/L, and total plate count reaches 10 11it is fermentation termination during cfu/L;
(3) after-ripening and seasoning: the product reaching fermentation termination is left standstill in 4 DEG C and carries out after-ripening in 12 hours, be beneficial to the formation of fragrance.The acesulfame potassium of the honey element of 0.04kg and 0.025kg is used in advance the water-soluble solution of 1kg, 95 DEG C of insulations sterilization in 20 minutes, to join in fermented product in sterile working mode and mix;
(4) packaging and preserving: sterile filling 4 DEG C of circulations, to store, shakes up before drinking.30 days shelf-lifves, pH≤3.0 in the shelf-life, total acid (in lactic acid) 5 ~ 9 g/L, total plate count 10 11cfu/L.

Claims (6)

1. prepare a method for pollen active probiotic drink, it is characterized in that,
(1) raw material is got by following weight parts: Bee Pollen 3 ~ 10, maltose and/or galactolipin 3 ~ 5, water 100, honey element 0.030 ~ 0.065, acesulfame potassium 0.020 ~ 0.030;
(2) preparation of fermentation medium: add water by after Bee Pollen and maltose and/or galactolipin mixing, stirs and by gauze or 4 layers of filtered through gauze;
(3) ferment: by fermentation medium high temperature sterilization, and be cooled to less than 37 DEG C; Then the mixed bacteria adding fermentation medium volume 3 ~ 5% expands liquid, and 30 ~ 37 DEG C of sealing and standing are cultivated; Treat that zymotic fluid pH is down to 3.10 ~ 3.30, total acid reaches 5 ~ 7 g/L, and total plate count reaches 10 11it is fermentation termination during cfu/L;
Wherein mixed bacteria expands liquid is expand liquid with lactobacillus BL1 bacterial classification expansion liquid and lactobacillus BL2 bacterial classification to add by after equal-volume mixing;
(4) after-ripening and seasoning: the product reaching fermentation termination is left standstill in 4 ~ 8 DEG C and carries out after-ripening in 8 ~ 12 hours; By honey element and acesulfame potassium water-soluble solution in advance, high temperature sterilization, to join in fermented product in sterile working mode and mixes rear sterile filling.
2. prepare the method for pollen active probiotic drink as claimed in claim 1, it is characterized in that, the preparation method that described lactobacillus BL1 bacterial classification expands liquid is:
(1) lactobacillus BL1 probio activation: cultivations of rule on MRS solid medium by lactobacillus BL1, is separated single bacterium colony and transfers MRS slant medium, and 37 DEG C of cultivations 24 ~ 72 hours are for subsequent use;
(2) preparation of lactobacillus BL1 liquid spawn: by inclined plane inoculating in liquid MRS culture medium, cultivates 18 ~ 24 hours, obtains liquid spawn for 37 DEG C;
(3) lactobacillus BL1 bacterial classification expands the preparation of liquid: compound concentration is the Bee Pollen aqueous solution of 3 ~ 5wt%, 115 DEG C of sterilizing 15 ~ 20min, are cooled to normal temperature, with the volume ratio inoculation liquid spawn of 3 ~ 5%, 37 DEG C of quiescent culture 1 ~ 2 day, obtain lactobacillus BL1 bacterial classification and expand liquid;
Described lactobacillus BL2 bacterial classification expands the preparation method of preparation method with lactobacillus BL1 bacterial classification expansion liquid of liquid.
3. prepare the method for pollen active probiotic drink as claimed in claim 1 or 2, it is characterized in that, the inoculation volume ratio that described mixed bacteria expands liquid is 4%.
4. prepare the method for pollen active probiotic drink as claimed in claim 1 or 2, it is characterized in that, described Bee Pollen removal of impurities also pulverized 40 ~ 80 mesh sieves, and 40 mesh sieves pulverized by maltose and/or galactolipin.
5. prepare the method for pollen active probiotic drink as claimed in claim 1 or 2, it is characterized in that, described fermented and cultured carries out high temperature sterilization in 15 ~ 30 minutes based on 115 DEG C of insulations.
6. prepare the method for pollen active probiotic drink as claimed in claim 1 or 2, it is characterized in that, described by after honey element and the dissolving of acesulfame potassium water in advance, within 20 minutes, carry out high temperature sterilization in 95 DEG C of insulations.
CN201310578456.1A 2013-11-19 2013-11-19 Pollen active probiotic drink and preparation method thereof Active CN103598654B (en)

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CN104855763B (en) * 2015-05-20 2018-02-02 浙江亚林生物科技股份有限公司 A kind of method for improving Bee Pollen flavor using probiotics
CN106615629A (en) * 2016-12-23 2017-05-10 江西领药业有限公司 Bee larva probiotic oral liquid for animal healthcare
CN109331045A (en) * 2018-11-23 2019-02-15 福建省微生物研究所 It is a kind of containing Bee Pollen, the probiotic composition of active carbon and preparation method thereof
CN109892660A (en) * 2019-03-04 2019-06-18 赵福振 A kind of production method of biologically active nanometer calcium
CN114431454B (en) * 2021-09-21 2024-06-25 株式会社APlCARE Preparation method of bee pollen ferment
CN115336693A (en) * 2022-08-25 2022-11-15 齐鲁工业大学 Bee pollen fermented sports beverage and preparation method thereof

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