CN116491575A - Feed additive for promoting absorption by utilizing vitamins and beneficial bacteria and preparation method thereof - Google Patents
Feed additive for promoting absorption by utilizing vitamins and beneficial bacteria and preparation method thereof Download PDFInfo
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- CN116491575A CN116491575A CN202310671342.5A CN202310671342A CN116491575A CN 116491575 A CN116491575 A CN 116491575A CN 202310671342 A CN202310671342 A CN 202310671342A CN 116491575 A CN116491575 A CN 116491575A
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- China
- Prior art keywords
- vitamin
- feed additive
- preparing
- bagasse
- clostridium butyricum
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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- A—HUMAN NECESSITIES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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Abstract
The invention discloses a feed additive for promoting absorption by utilizing vitamins and beneficial bacteria and a preparation method thereof, wherein the feed additive is prepared from the following raw materials in parts by weight: vitamin A, vitamin B2, vitamin D3, clostridium butyricum and the like, and the microorganisms are embedded to obtain powder with raised surface fibers. The preparation method comprises the following steps of (1) preparing plant fiber powder; (2) alkali washing; (3) activating and culturing strains; (4) strain attachment culture; (5) embedding; (6) freeze-drying and pulverizing. The intestinal flora is improved by improving the absorption of food in a way of improving the intestinal flora, on one hand, beneficial bacteria stay in the intestinal tract for a longer time, so that the beneficial microorganisms are recovered from a dormant state, auxiliary materials provide proper nutrition conditions and environment, and are amplified in proper intestinal environment to replace the beneficial bacteria and inhibit the growth of mixed bacteria, thereby improving the intestinal flora.
Description
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a feed additive for promoting absorption by utilizing vitamins and beneficial bacteria and a preparation method thereof.
Background
The antibiotics are used as feed additives for improving the growth efficiency of livestock and poultry, promote the development of the breeding industry, but are unreasonably and uncontrollably used for a long time, so that multi-drug resistant bacteria are generated.
At present, certain achievements are achieved in the aspect of researching a replacement antibody product, antibiotics can be replaced to a certain extent by probiotics, traditional Chinese medicine extracts, antibacterial peptides, enzyme preparations and the like, probiotics refer to active microorganisms which are planted in an animal body and are beneficial to the body, and the intestinal environment can be optimized by improving the immunity response capability to enhance the disease resistance of the body or by inhibiting the growth of harmful flora and regulating the composition of intestinal flora.
The spot inspection shows that the marked strains and the living bacteria content of various probiotic products are very different, which is one of the reasons for the quality difference of the probiotic products, so that the preparation process of the existing probiotic preparation is continuously improved, and the living bacteria content and the stability of the probiotic preparation are improved. The single tibody product has no obvious effect and can not meet the requirements of the breeding industry, and the composite probiotics can be used together or scientifically matched with other preparations, such as traditional Chinese medicine extracts and the like, so that the composite probiotics can exert the maximum effect.
The bulletin of the Ministry 194 clearly indicates that antibiotics which can play a role in promoting the growth of animals are forbidden to be used in animal feed, the cultivation resistance reduction is realized gradually, the feed is forbidden, and the product is free of resistance. The microbial improvement of the gastrointestinal flora of livestock is beneficial to improving the absorption capacity of livestock corresponding to the feed, but the feed additive is decomposed when passing through gastric acid environment and intestinal environment, the microbial deactivation is carried out, and meanwhile, the stay time period of the microbial in the intestinal tract is insufficient to improve the species of the gastrointestinal flora.
Disclosure of Invention
The invention aims to provide a feed additive which utilizes vitamins and beneficial bacteria to promote absorption, improves the activity of microorganisms after the feed additive is adhered and cultured, improves the adhesion of the microorganisms in intestinal tracts, slows down the moving speed, finishes recovery in the intestinal tracts to the maximum extent and improves the gastrointestinal flora.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the feed additive for promoting absorption by utilizing vitamins and beneficial bacteria is prepared from the following raw materials in parts by weight: vitamin A, vitamin B2, vitamin D3, clostridium butyricum, glucose, anhydrous sodium sulfate, plant fibers, other microorganism components and auxiliary materials, and microorganisms are embedded to obtain powder with raised surface fibers.
Further, 1000 g: vitamin A > 15000001U and vitamin B 2 > 2000mg, vitamin D 3 More than or equal to 400000IU, clostridium butyricum more than or equal to 1.0X10 7 CFU/g。
The preparation method of the feed additive for promoting absorption by utilizing vitamins and beneficial bacteria comprises the following steps,
(1) Preparing plant fiber powder: preparing bagasse as a raw material, degumming the bagasse, drying the bagasse to a water content of 30-40%, freezing and storing the bagasse for 150-200h at a temperature of-10 ℃, and grinding the bagasse by external force to obtain fiber powder with 200-300 meshes, so that a large number of cracks are generated among fibers;
(2) Alkali washing: washing mechanically degummed bagasse with alkali solution to prepare NaOH solution with concentration of 20g/L, mixing the fiber powder (g) obtained in step (1) with NaOH (cm) 3 ) Soaking the solution according to the proportion of 1:10, heating and preserving heat at 50 ℃ for 1h, washing with clear water after finishing, sterilizing with high-temperature steam and softening fiber at 80-100 ℃ for 20-30min to obtain softened fiber;
(3) Activating and culturing strains: preparing a liquid culture medium, wherein the liquid culture medium comprises the following components in parts by weight (g/L): 8-10 parts of glucose, 15-25 parts of corn starch, 0.2-0.3 part of NaCl, 2.0-2.2 parts of clostridium butyricum, 0.5-0.8 part of lactobacillus, 0.3-0.5 part of beer yeast powder and 30-38 ℃ for anaerobic culture for 48 hours to obtain the clostridium butyricum with the viable count of 8 multiplied by 10 8 CFU/mL bacterial liquid;
(4) And (3) strain attachment culture: mixing bran and softened fibers obtained in the step (2) according to a ratio of 3:1, adding 40-50% of bacterial liquid obtained in the step (3) by weight percent, fully stirring and uniformly mixing to obtain slurry, carrying out anaerobic culture for 20-25h at a temperature of 30-38 ℃, carrying out attachment culture by a shaking table, and adding vitamin A, vitamin B2, vitamin D3, glucose and anhydrous sodium sulphate after completion of the attachment culture, and uniformly mixing to obtain paste;
(5) Embedding: dissolving the embedding material in 70 ℃ warm water to prepare uniform suspension, loading the suspension into a sprayer, spraying the embedding material into the paste obtained in the step (4) while stirring, and fully stirring.
(6) Freeze drying and pulverizing: adding a freeze-drying protective agent, namely respectively preparing solution with concentration of 10% by using inulin, PEG6000, skimmed milk powder and glycerol, adding the solution as the freeze-drying protective agent into the embedded paste obtained in the step (5), uniformly mixing the materials according to the ratio of 1:2, spreading the materials after uniformly mixing, and performing vacuum freeze-drying under the following conditions: pre-freezing at-70deg.C for 2 hr, freezing at-50deg.C under vacuum degree of 10Pa for 20 hr, repeating for 3 times, and grinding and pulverizing into powder.
Clostridium butyricum is a gram-positive anaerobic bacterium belonging to the genus clostridium of the family armillariaceae. Clostridium butyricum has stronger resistance to the external environment, can secrete amylase in animal intestinal tracts, decompose carbohydrates to generate butyric acid, inhibit pathogenic bacteria such as staphylococcus aureus and the growth of harmful microorganisms, can improve the immunity and antioxidation of animal organisms, has development prospects in the aspects of animal nutrition utilization and pathogen inhibition, and has research value in animal husbandry and food industry.
Under in-vivo conditions, clostridium butyricum can resist gastric acid and digestive juice, and clostridium butyricum and preparations thereof are stable at room temperature and are easy to store and transport; promoting nutrition metabolism, inhibiting in vivo pathogenic bacteria, regulating intestinal microorganism balance, enhancing animal immunity, and enhancing antioxidant capacity. Clostridium butyricum not only can inhibit the massive proliferation of pathogenic bacteria in the intestinal tract, but also can promote the proliferation and development of beneficial flora, improve the unbalanced condition of the flora in the intestinal tract and reduce enterotoxins, and clostridium butyricum can inhibit the production of toxic proteins by closely contacting clostridium difficile.
Clostridium butyricum has the properties of acid resistance and high temperature resistance, has a good growth inhibition effect on pathogens in intestinal tracts, such as escherichia coli, shigella and salmonella, can obviously promote the growth and development of beneficial bacteria in the intestinal tracts, and can obviously increase the viable count of lactobacillus acidophilus, streptococcus faecalis and bifidobacteria by a sample extracted from fermentation liquor of clostridium butyricum. The probiotics characteristics of clostridium butyricum enable the clostridium butyricum to be applied to animal feed additives to improve animal nutrition conditions and improve feed utilization rate.
The clostridium butyricum can also produce and secrete short-chain fatty acids, the short-chain fatty acids can be directly absorbed in the gastrointestinal tract of animals, so that the peristalsis of the intestinal tract is accelerated, and the clostridium butyricum can secrete amino acids, vitamin K, amylase, pectinase and other substances, so that the proliferation of probiotic bacteria in the intestinal tract is facilitated.
The invention has the following beneficial effects:
1. supplement multiple vitamins, reduce stress reaction, and improve immunity.
2. Improving reproductive capacity of female livestock and fowl, increasing farrowing rate, survival rate, fertilization rate, hatching rate, and improving sperm activity of breeding livestock and fowl.
3. Improving eggshell quality, reducing soft shell egg, thin shell egg and sand shell egg occurrence, increasing eggshell glossiness, and prolonging egg laying peak.
4. Improving the glossiness of the livestock and poultry fur and improving the quality of the fur; reduce the occurrence of pig, cattle and sheep hoof cracking.
5. Adjusting the structure of flora in the intestinal tracts of livestock and poultry, promoting the growth of beneficial bacteria, inhibiting the growth of harmful bacteria, reducing the occurrence of intestinal diseases and reducing the occurrence of constipation.
6. The thickness of the intestinal mucosa is cultivated, the intestinal mucosa has certain repairing and regeneration promoting effects on the damaged intestinal mucosa, the ingestion and the digestion and the absorption of livestock are improved, the feces are fine and smooth, the feed utilization rate is improved, and the growth speed of the livestock is improved.
7. Improving lactation ability of lactating animals and enriching milk nutrition structure.
The usage amount is as follows: the product is 500g mixed with 2000kg of water or 1000kg of mixed material, and can be eaten or drunk freely. The long-term use has better effect. The preparation can be used in a doubling amount during the onset period to reduce stress reaction. Is favorable for recovery of illness.
Drawings
In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings used for describing the embodiments will be briefly described below.
Fig. 1: schematic representation of bagasse fibers of the present invention in microscopic state before and after treatment.
Fig. 2: the plant fiber schematic diagram of the particle surface softening under the microscopic state of the feed powder.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
The feed additive for promoting absorption by utilizing vitamins and beneficial bacteria is prepared from the following raw materials in parts by weight: vitamin A, vitamin B2, vitamin D3, clostridium butyricum, glucose, anhydrous sodium sulfate, plant fibers, other microorganism components and auxiliary materials, and microorganisms are embedded to obtain powder with raised surface fibers.
1000 g: vitamin A > 15000001U and vitamin B 2 > 2000mg, vitamin D 3 More than or equal to 400000IU, clostridium butyricum more than or equal to 1.0X10 7 CFU/g。
The preparation method of the feed additive for promoting absorption by using vitamins and beneficial bacteria comprises the following steps of,
as shown in fig. 1-2: (1) preparing plant fiber powder: preparing bagasse as a raw material, degumming the bagasse, drying the bagasse to a water content of 30-40%, freezing and storing the bagasse for 150-200h at a temperature of-10 ℃, and grinding the bagasse by external force to obtain fiber powder with 200-300 meshes, so that a large number of cracks are generated among fibers; the cellulose molecular chain in the raw hemp polymer is sheared for a plurality of times, so that brittle colloid in bagasse is broken and peeled off, and fibers are separated from colloid. The individual microscopic lengths under microscopic observation are 0.1-0.2mm. The natural cold energy generated under the low temperature condition carries out long-term freezing treatment on the fibrilia, on one hand, bacteria propagated in the natural condition are utilized to decompose colloid in the fibrilia, and on the other hand, micro cracks are generated among different components by cold and hot impact caused by temperature difference change, so that the cellulose and non-cellulose in the fibrilia generate structural damage, and further treatment is facilitated.
(2) Alkali washing: washing mechanically degummed bagasse with alkali solution to prepare NaOH solution with concentration of 20g/L, mixing the fiber powder (g) obtained in step (1) with NaOH (cm) 3 ) Soaking the solution according to the proportion of 1:10, heating and preserving heat at 50 ℃ for 1h, washing with clear water after finishing, sterilizing with high-temperature steam and softening fiber at 80-100 ℃ for 20-30min to obtain softened fiber; by utilizing the difference of the thermal expansion coefficients of the fiber and the components and the matrix, the fiber is placed in a freezing environment, the relative displacement is generated between the fiber and the components, the surface of the fiber is bent to a certain extent, the surface area of the cellulose is increased, the surface roughness is improved by alkali washing, the interface bonding of the matrix is facilitated, and the interface bonding performance of the fiber and the adhesive force between the matrix are improved.
(3) Activating and culturing strains: liquid culture is configuredA base comprising the following components (g/L): glucose 8-10, corn starch 15-25, naCl0.2-0.3, clostridium butyricum bacterial agent 2.0-2.2, lactobacillus bacterial agent 0.5-0.8, beer yeast powder 0.3-0.5, anaerobic culture at 30-38deg.C for 48 hr to obtain clostridium butyricum viable count of 8X10 8 CFU/mL bacterial liquid; carbon dioxide is an important condition in the culturing process of clostridium butyricum, and in an anaerobic environment, if the culturing condition does not contain carbon dioxide, the growth of clostridium butyricum is delayed or even stopped; carbon dioxide can be generated in the growth process of the lactobacillus brevis, which is favorable for starting the growth of clostridium butyricum, so that the co-culture of clostridium butyricum and lactobacillus brevis is feasible, which also indicates that clostridium butyricum can be used for preparing a composite preparation with other strains, and the clostridium butyricum and clostridium butyricum can be applied to the aspects of medicines, feeds and the like.
Lactic acid bacteria not only improve intestinal flora, but also are beneficial to flora in liquid feed, and are used for reducing degradation of microorganisms on feed in terms of animal nutrition, and bad effects of poor feed sanitation are mainly caused by metabolites of the microorganisms, and the microorganisms utilize nutrients in the feed, so that the nutritional value of the feed is reduced. The rapid propagation of lactic acid bacteria produces a large amount of lactic acid, which lowers the pH of the feed, whereas acidification of the feed is beneficial for the distribution of microorganisms in the feeding system and the animal intestinal tract, and competition of lactic acid bacteria with harmful microorganisms inhibits their propagation.
(4) And (3) strain attachment culture: mixing bran and softened fibers obtained in the step (2) according to a ratio of 3:1, adding 40-50% of bacterial liquid obtained in the step (3) by weight percent, fully stirring and uniformly mixing to obtain slurry, carrying out anaerobic culture for 20-25h at a temperature of 30-38 ℃, carrying out attachment culture by a shaking table, and adding vitamin A, vitamin B2, vitamin D3, glucose and anhydrous sodium sulphate after completion of the attachment culture, and uniformly mixing to obtain paste; the bran has the highest water absorption rate, the bran is rich in protein, polysaccharide and vitamin, and the deep processing and utilization of the bran are getting more and more attention.
(5) Embedding: dissolving the embedding material in 70 ℃ warm water to prepare uniform suspension, loading the suspension into a sprayer, spraying the embedding material into the paste obtained in the step (4) while stirring, and fully stirring. The embedding material is acacia or gelatin.
(6) Freeze drying and pulverizing: preparing a freeze-drying protective agent, adding the freeze-drying protective agent into the embedded paste obtained in the step (5), uniformly mixing according to the ratio of 1:2, and spreading the paste after uniformly mixing, wherein the vacuum freeze-drying conditions are as follows: pre-freezing at-70deg.C for 2 hr, freezing at-50deg.C under vacuum degree of 10Pa for 20 hr, repeating for 3 times, and grinding and pulverizing into powder. Freeze-drying is a two-stage process in which the cells are first frozen rapidly at very low temperatures and then chilled water is removed by a slow evaporation process at low pressure and temperature, which removes most of the water from around and within the cells, leaving the microorganisms dormant.
And (3) preparing a solution with the concentration of 10% from inulin, PEG6000, skimmed milk powder and glycerol as the lyoprotectant in the step (6).
The main beneficial components of the feed additive for the gastrointestinal tract are vitamin A, vitamin B2, vitamin D3 and clostridium butyricum, wherein the clostridium butyricum proliferates and permeates on the bran in an adhesion culture mode. Meanwhile, bran is taken as basic glycogen, when the powder is prepared, bagasse fibers are added, the bagasse fibers contain high plant fibers and have strong toughness, fiber components with colloid removed from the surfaces of the fibers are obtained through extraction of the bagasse fibers, the surfaces of the fibers are in a concave-convex incomplete state through microscopic observation, the adhesion is improved, the combination property is improved conveniently, the bran and the bran can be combined effectively, the bran and the bagasse fibers are embedded by an embedding agent and ground into powder, the powder particles are not easy to loosen and are in finer particles, meanwhile, the surfaces are provided with the plant fibers, when the bran and the bagasse fibers are used for additives, the surface friction is improved well, the bran and the bran are not easy to be subjected to intestinal peristalsis, and the bran are discharged rapidly, so that the time of the additives staying in intestinal tracts is prolonged.
The invention promotes the absorption of food by improving the intestinal flora, so as to improve the intestinal flora, on one hand, beneficial bacteria stay in the intestinal tract for a longer time, the beneficial microorganisms are revived from a dormant state, auxiliary materials provide proper nutrition conditions and environment, and the auxiliary materials are amplified in proper intestinal environment to complete the replacement of the beneficial bacteria and inhibit the growth of mixed bacteria, so that the gastrointestinal flora is improved.
In order to ensure that clostridium butyricum stays in the intestinal tract for a longer time, the clostridium butyricum is amplified, subjected to adhesion culture and embedded to finally form powder, wherein the powder is granular, and plant fibers and softened plant fibers invisible to naked eyes are arranged on the surface of the powder, so that the adhesion force can be improved, the moving speed of an additive in the intestinal tract can be slowed down, and the intestinal flora can be improved.
And finally, pulverizing by a freeze-drying mode, wherein the freeze-drying mode can furthest maintain the activity of microorganisms on one hand, and simultaneously enables the microorganisms to enter a dormant state, so that the recovery in an intestinal environment is facilitated, and meanwhile, a cavity effect is easy to form by the freeze-drying mode, so that the pulverization and the grinding are facilitated, and the damage to fibers on the surfaces of particles due to the mechanical grinding effect is prevented.
Test detection effect: the chicken feed is fed to chickens, and anatomical analysis shows that the intestinal villi height of the chickens in the clostridium butyricum added group is obviously higher than that in the control group, the daily gain of the chickens is also obviously higher than that in the control group, and meanwhile, the morbidity of the clostridium butyricum added group is also lower, so that the antibiotic requirement is indirectly reduced.
According to research, the clostridium butyricum is added into the feed for the broiler chickens, so that the pH value in the cecum of the broiler chickens can be improved, the length of the cecum is shortened, the alkaline phosphatase activity in the jejunum of the broiler chickens is improved, the content of short-chain fatty acid in the cecum is increased, and the clostridium butyricum has positive influence on the intestinal health of the broiler chickens.
Clostridium butyricum can help the breeding ducks to absorb various elements such as calcium, iron, phosphorus, copper, zinc, manganese and the like in the feed through endocrine vitamins and enzymes in the intestinal tracts of the breeding ducks, promote sexual development and maturation of the breeding ducks, improve reproductive capacity of the breeding ducks, and improve semen quality of the breeding ducks and fertilization rate of female ducks. The clostridium butyricum preparation can achieve the purpose of preventing and treating partial diseases by regulating the balance of intestinal flora, can also reduce the medicine residue in animals, reduce the double infection rate, promote the animals to absorb proteins, increase the protein nitrogen content in blood, reduce cholesterol, produce beneficial substances such as clostridium butyricum, butyric acid and the like, promote the nutrition absorption and metabolism of animals, maintain the operation of organisms and improve the quality of animal products.
The feed additive disclosed by the invention can be added into broiler feed in a proper amount to enhance the activity of broiler lipoprotein lipase, enhance the expression of fatty acid binding protein in jejunum, and remarkably increase the contents of intramuscular dodecapentenoic acid and unsaturated fatty acid, so that clostridium butyricum can be proved to be capable of improving the quality of animal meat products.
In the actual use process, the effect on fattening pigs and sows is found to be good, and the provided softening fiber can improve dietary fiber and improve meat compactness; improving sow reproductive function.
The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention.
Claims (4)
1. The feed additive for promoting absorption by utilizing vitamins and beneficial bacteria is characterized in that:
the composite material is prepared from the following raw materials in parts by weight: vitamin A, vitamin B2, vitamin D3, clostridium butyricum, glucose, anhydrous sodium sulfate, plant fibers, other microorganism components and auxiliary materials, and microorganisms are embedded to obtain powder with raised surface fibers.
2. The feed additive for promoting absorption using vitamins and beneficial bacteria according to claim 1, wherein: 1000 g: vitamin A > 15000001U and vitamin B 2 > 2000mg, vitamin D 3 More than or equal to 400000IU, clostridium butyricum more than or equal to 1.0X10 7 CFU/g。
3. A method for preparing a feed additive for promoting absorption by vitamins and beneficial bacteria, which is used for preparing the feed additive for promoting gastrointestinal absorption according to claim 1, and is characterized in that: comprises the following steps of the method,
(1) Preparing plant fiber powder: preparing bagasse as a raw material, degumming the bagasse, drying the bagasse to a water content of 30-40%, freezing and storing the bagasse for 150-200h at a temperature of-10 ℃, and grinding the bagasse by external force to obtain fiber powder with 200-300 meshes, so that a large number of cracks are generated among fibers;
(2) Alkali washing: washing mechanically degummed bagasse with alkali solution to prepare NaOH solution with concentration of 20g/L, mixing the fiber powder (g) obtained in step (1) with NaOH (cm) 3 ) Soaking the solution according to the proportion of 1:10, heating and preserving heat at 50 ℃ for 1h, washing with clear water after finishing, sterilizing with high-temperature steam and softening fiber at 80-100 ℃ for 20-30min to obtain softened fiber;
(3) Activating and culturing strains: preparing a liquid culture medium, wherein the liquid culture medium comprises the following components in parts by weight (g/L): 8-10 parts of glucose, 15-25 parts of corn starch, 0.2-0.3 part of NaCl, 2.0-2.2 parts of clostridium butyricum, 0.5-0.8 part of lactobacillus, 0.3-0.5 part of beer yeast powder and 30-38 ℃ for anaerobic culture for 48 hours to obtain the clostridium butyricum with the viable count of 8 multiplied by 10 8 CFU/mL bacterial liquid;
(4) And (3) strain attachment culture: mixing bran and softened fibers obtained in the step (2) according to a ratio of 3:1, adding 40-50% of bacterial liquid obtained in the step (3) by weight percent, fully stirring and uniformly mixing to obtain slurry, carrying out anaerobic culture for 20-25h at a temperature of 30-38 ℃, carrying out attachment culture by a shaking table, and adding vitamin A, vitamin B2, vitamin D3, glucose and anhydrous sodium sulphate after completion of the attachment culture, and uniformly mixing to obtain paste;
(5) Embedding: dissolving the embedding material in 70 ℃ warm water to prepare uniform suspension, loading the suspension into a sprayer, spraying the paste obtained in the step (4) into the embedding material while stirring, and fully stirring;
(6) Freeze drying and pulverizing: preparing a freeze-drying protective agent, adding the freeze-drying protective agent into the embedded paste obtained in the step (5), uniformly mixing according to the ratio of 1:2, and spreading the paste after uniformly mixing, wherein the vacuum freeze-drying conditions are as follows: pre-freezing at-70deg.C for 2 hr, freezing at-50deg.C under vacuum degree of 10Pa for 20 hr, repeating for 3 times, and grinding and pulverizing into powder.
4. A method of preparing a feed additive for promoting absorption using vitamins and beneficial bacteria according to claim 3, characterized in that: and (3) preparing a solution with the concentration of 10% from inulin, PEG6000, skimmed milk powder and glycerol as the lyoprotectant in the step (6).
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| CN117562189A (en) * | 2024-01-15 | 2024-02-20 | 中国农业大学 | Application of vitamin K in preparing feed for regulating intestinal flora of broiler chickens |
| CN117562189B (en) * | 2024-01-15 | 2024-04-05 | 中国农业大学 | Application of vitamin K in preparing feed for regulating intestinal flora of broiler chickens |
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