CN116430058B

CN116430058B - Immunoassay equipment for tumor screening

Info

Publication number: CN116430058B
Application number: CN202310704590.5A
Authority: CN
Inventors: 巩赞华; 巩赞斌; 巩赞博; 彭劼; 张金银
Original assignee: Shenzhen Pandao Medical Laboratory; Huachenyang Shenzhen Technology Co ltd
Current assignee: Shenzhen Pandao Medical Laboratory; Huachenyang Shenzhen Technology Co ltd
Priority date: 2023-06-14
Filing date: 2023-06-14
Publication date: 2023-08-18
Anticipated expiration: 2043-06-14
Also published as: CN116430058A

Abstract

The invention relates to the field of medical equipment, and discloses immunity analysis equipment for tumor screening, which comprises a machine shell, wherein a sample injection mechanism is arranged on the machine shell, the sample injection mechanism comprises a mounting table arranged on the side surface of the machine shell, a sample injection port is arranged on the mounting table, a sample injection hole is arranged on the side surface of the machine shell, the lower orifice of the sample injection port is attached to the upper orifice of the sample injection hole, the sample injection mechanism further comprises synchronous belts arranged on the mounting table, two groups of synchronous belts are arranged, the sample injection port is positioned between the two groups of synchronous belts, traction grooves are arranged on the outer surfaces of the synchronous belts in a penetrating way, the traction grooves are in an arc groove shape, a plurality of traction grooves are arranged in an array along the extending direction of the synchronous belts, and when a test tube enters the machine shell through the sample injection port, the outer wall of the test tube is wiped by disinfection and sterilization mediums such as alcohol sprayed on the surface of the synchronous belts in advance, so as to achieve the disinfection and sterilization effects.

Description

Immunoassay equipment for tumor screening

Technical Field

The invention relates to the field of medical equipment, in particular to immunity analysis equipment for tumor screening.

Background

When diagnosing tumor patients, the patients need to be subjected to immune detection, and an immune detection analysis instrument is various detection and analysis devices established by taking immune response specificity as a principle, and can automatically complete a series of operation steps of sample adding, reagent adding, incubation reaction, cleaning, detection and the like, wherein: 1. the sample loading step is to put samples into test tubes, then put multiple groups of test tubes into test tube racks one by one, open a sample cabin of an immunoassay device, put the test tube racks into the sample cabin, close a cabin cover of the sample cabin, push the test tubes in the sample cabin into the device through a pushing structure, and in the process, medical staff take gloves to manually put the test tubes into the test tube racks, so that the fingerprints, sweat stains and the like of the medical staff are left on the outer walls of the test tubes due to incorrect operation, or the glove is slightly damaged, or the sweat stains overflow the glove, and the like, and the test tubes enter the device along with the test tubes, so that pollution is caused to the detection environment in the device; 2. in the detection process, after the probe for sucking the sample is used once, the probe is required to be cleaned once, in the prior art, the probe is generally pulled to stretch into a cleaning medium, such as an aqueous medium, and then the probe is quickly cleaned in a vibration mode and the like, the outer wall of the probe can be effectively cleaned by the cleaning mode, but the cleaning effect of the inner wall of the probe is not as good as that of the outer wall, in addition, after the cleaning is finished, the probe leaves the cleaning medium, a little cleaning medium is remained and attached to the inner and outer sides of the probe, and the accuracy of a subsequent detection result is easily influenced.

Based on the above, the invention provides an immunoassay device for tumor screening.

Disclosure of Invention

In order to solve the problems mentioned in the background above, the present invention provides an immunoassay device for tumor screening.

In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows.

The utility model provides an immunity analysis equipment for tumour screening, which comprises a housing, install the sampling mechanism on the casing, sampling mechanism is including setting up the mount table on the casing side, be provided with the sample inlet on the mount table, the side of casing is provided with the sample inlet, the lower drill way of sample inlet is laminated with the last drill way of sample inlet, sampling mechanism is still including setting up the hold-in range on the mount table, the hold-in range is provided with two sets of and sample inlet is located between two sets of hold-in ranges, the surface of hold-in range runs through and is provided with the traction groove, the traction groove is the arc groove shape, the traction groove is provided with a plurality of along the extending direction array of hold-in range.

Further, be provided with the application of sample device in the casing, the application of sample device includes receiving mechanism and presss from both sides and gets the mechanism, presss from both sides and gets the mechanism including setting up the fixed bolster in the casing and installing the rotatory ring frame in the fixed bolster upper end, the outer wall of rotatory ring frame is provided with and presss from both sides and get the component, presss from both sides and gets the component and be provided with the multiunit along the circumferencial direction array of rotatory ring frame, receiving mechanism and clamp the cooperation of getting the component and receive and press from both sides the test tube and get.

Further, a cleaning mechanism is arranged in the shell and comprises a sampling needle, the sampling needle comprises an outer mounting sleeve, the upper end of the outer mounting sleeve is closed, a sampling tube is mounted inside the sampling needle through an inner support, the sampling tube is coaxial with the outer mounting sleeve, the lower end of the sampling tube extends out of the outer mounting sleeve, a liquid level detection needle is coaxially arranged in the sampling tube, the upper end of the liquid level detection needle extends out of the outer mounting sleeve and is in lifting arrangement, and in an initial state, the lower end of the liquid level detection needle and the lower end of the outer mounting sleeve are positioned on the same horizontal plane;

the outer circular surface of the outer mounting sleeve is provided with a joint, the upper end of the liquid level detection needle is opened, the lower end of the liquid level detection needle is closed, and the outer circular surface is provided with a water outlet hole;

the cleaning mechanism further comprises a switching valve, an air pump, a cleaning medium source, a hot air source and a cold air source, wherein the switching valve is arranged in the extraction state, the cleaning state, the drying state and the cooling state for switching, when the cleaning mechanism is in the extraction state, the switching valve is used for communicating the air pump with the liquid level detection needle, when the cleaning mechanism is in the cleaning state, the switching valve is used for communicating the cleaning medium source with the connector and the liquid level detection needle, when the cleaning mechanism is in the drying state, the switching valve is used for communicating the hot air source with the connector and the liquid level detection needle, and when the cleaning mechanism is in the cooling state, the switching valve is used for communicating the cold air source with the connector and the liquid level detection needle.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, when the test tube enters the shell through the sample inlet, the outer wall of the test tube is wiped by the disinfection and sterilization medium such as alcohol sprayed on the surface of the synchronous belt in advance, so that the disinfection and sterilization effect is achieved, and the problem that fingerprints or sweat stains and the like left on the outer wall of the test tube influence the detection environment when medical staff take the test tube is solved.

2. Based on one, be provided with in the casing and be used for receiving the test tube and press from both sides the application of sample device that gets the test tube and carry out orderly storage, the application of sample device is through the order of motor two, can realize following action: the receiving sleeve receives the test tube, the clamping arms open, the traction test tube moves to between the two groups of clamping arms, the clamping arms fold to clamp the test tube, and the test tube is separated from the receiving sleeve, so that the five transfer actions form a complete clamping action of the test tube, the action and the action are connected smoothly without any pause, the efficiency is high, and the driving mode of a single power source is relatively energy-saving.

3. In the invention, four actions of negative pressure extraction, cleaning, drying and cooling of the sampling needle can be respectively realized through one switching valve, and the negative pressure extraction device has the significance that in the prior art, a person skilled in the art can easily think that a plurality of electromagnetic valves are matched with a plurality of pipelines to realize the same effects, but: on one hand, control switching of a plurality of electromagnetic valves is realized through a preset program, the preset program is complex and tedious, the opening and closing of the electromagnetic valves are not disconnected, the time is delayed, the cleaning efficiency is influenced, and the detection efficiency is further influenced; by contrast, the invention can realize four actions of negative pressure extraction, cleaning, drying and cooling on the sampling needle through a group of switching valves, has no redundant flow track and has higher cleaning efficiency.

Drawings

FIG. 1 is a schematic diagram of the structure of the present invention;

FIG. 2 is an internal schematic diagram of the present invention;

FIG. 3 is a schematic diagram of a sample injection mechanism;

fig. 4 is a top view of the timing belt, the draw trough and the test tube;

FIG. 5 is a schematic diagram of a sample application device;

FIG. 6 is a schematic illustration of the engagement of the receiving mechanism with the gripping member;

FIG. 7 is a second schematic illustration of the engagement of the receiving mechanism with the gripping member;

FIG. 8 is a third schematic illustration of the engagement of the receiving mechanism with the gripping member;

FIG. 9 is a schematic diagram showing the engagement of the receiving mechanism with the gripping member;

FIG. 10 is a partial schematic view of a crank member;

FIG. 11 is a partial schematic view of a crank member II;

fig. 12 is a schematic structural view of the receiving member;

FIG. 13 is a partial schematic view of a receiving member;

FIG. 14 is a schematic view of the structure of the gripping member;

FIG. 15 is a partial schematic view of a gripping member;

FIG. 16 is a schematic diagram of a trigger block;

FIG. 17 is a schematic view of a clip arm;

FIG. 18 is a schematic diagram of a cleaning mechanism I;

FIG. 19 is a second schematic view of a cleaning mechanism;

FIG. 20 is a schematic view of a sampling needle;

FIG. 21 is a schematic diagram of a switching valve;

figure 22 is a cross-sectional view of the valve housing;

fig. 23 is a cross-sectional view of the outer valve sleeve and the inner valve core.

The reference numerals in the drawings are:

100. a housing; 200. a sample injection mechanism; 201. a mounting table; 202. a sample inlet; 203. a synchronous belt; 204. a traction groove; 300. a sample adding device; 301. a fixed bracket; 302. rotating the ring frame; 303. a first motor; 304. a telescopic rod; 400. a receiving mechanism; 401. a mounting bracket; 402. a ring groove; 403. a second motor; 404. swing arms; 405. a traction hole; 406. a slide pin; 407. a first guide rod; 408. a second guide rod; 409. a slide block; 410. a support rod; 411. a first spring; 412. a mounting base; 413. a guide rod III; 414. a pushing block; 415. pushing a frame; 416. a second spring; 417. a third spring; 418. a push rod; 419. a receiving sleeve; 500. a gripping member; 501. a connecting bracket; 502. a guide rod IV; 503. a trigger block; 5031. a cambered surface I; 5032. an inclined plane I; 5033. a transition surface; 5034. plane one; 5035. a cambered surface II; 5036. plane two; 5037. a second inclined plane; 504. a spring IV; 505. a spring V; 506. a chassis; 507. a guide rod V; 508. a spring six; 509. a clamping bracket; 510. a clamp arm; 511. a spring seven; 512. a sliding sleeve; 513. a slide bar; 514. a spring eight; 600. a cleaning mechanism; 601. a sampling needle; 6011. an outer mounting sleeve; 6012. a sampling tube; 6013. a liquid level probe; 6014. a water outlet hole; 6015. a joint; 602. a linear module; 603. a switching valve; 6031. a valve housing; 6032. a nozzle; 6033. a side double groove; 6034. a side single hole; 6035. an outer valve sleeve; 6036. an outer valve hole; 6037. an inner valve core; 6038. an internal valve hole; 604. an air extracting pump; 605. a first connecting pipe; 606. a second connecting pipe; 607. a fourth motor; 700. and a third motor.

Detailed Description

In order to further describe the technical means and effects adopted by the present invention for achieving the intended purpose, the following detailed description will refer to the specific implementation, structure, characteristics and effects according to the present invention with reference to the accompanying drawings and preferred embodiments.

Example 1

As shown in fig. 1 to 4, an immunoassay device for tumor screening includes a housing 100, and a sample injection mechanism 200 is mounted on the housing 100, and is used for drawing a test tube into the housing 100 and wiping and sterilizing the outer wall of the test tube by the sample injection mechanism 200 during the process of drawing the test tube into the housing 100.

As shown in fig. 3, the sample feeding mechanism 200 includes a mounting table 201 disposed on a side surface of the casing 100, a sample feeding port 202 is disposed on the mounting table 201, a sample feeding hole is disposed on a side surface of the casing 100, and a lower hole of the sample feeding port 202 is attached to an upper hole of the sample feeding hole, so that, except for the sample feeding port 202 and the sample feeding hole, no gap related to the entry of the test tube is left, and it should be noted that, in fig. 1, in order to show an internal structure of the casing 100, a glass window right in front of the casing 100 is hidden.

The sample feeding mechanism 200 further includes a synchronous belt 203 disposed on the mounting table 201, where the synchronous belt 203 is implemented in the prior art, and not described in detail, the synchronous belt 203 is provided with two groups, and the sample feeding port 202 is located between the two groups of synchronous belts 203.

The outer surface of the synchronous belt 203 is provided with traction grooves 204 in a penetrating manner, the traction grooves 204 are arc-shaped, and the traction grooves 204 are provided in a plurality along the extending direction of the synchronous belt 203 in an array manner.

When in use, test tubes are firstly placed into two traction grooves 204 which are oppositely arranged in two groups of synchronous belts 203 one by one, and when in placement: the medical staff generally holds the tube orifice of the test tube by the thumb and the index finger, when the test tube is taken in, the distance direction between the thumb and the index finger is parallel to the distance direction between the two groups of synchronous belts 203, namely after the test tube is put in, the part of the test tube held by the thumb and the index finger is positioned right above the traction groove 204;

then, hold-in range 203 operation pulls the test tube to remove, when the test tube removes to sample inlet 202 department, hold-in range 203 pauses, and the test tube drops under the action of gravity, gets into in the casing 100 through sample inlet 202, and the outer wall of test tube can be with traction groove 204 cell wall contact, cleans the test tube outer wall through spraying disinfection medium such as alcohol on the hold-in range 203 surface in advance, plays the disinfection and sterilization effect, so solved "when medical personnel gets the test tube, the fingerprint or sweat stain etc. that leave at the test tube outer wall cause the problem of" to detect the environment.

In the preferred embodiment, as shown in fig. 4, the opposite sides of the two synchronous belts 203 are attached, and the diameter of the traction groove 204 is identical to that of the test tube, which means that the whole outer wall of the test tube is wrapped by the whole hole formed by the two traction grooves 204, so that the position of the test tube when the test tube is put in is not needed to be considered, and when the test tube is inserted into the traction groove 204 and falls from the traction groove 204, all parts of the outer wall of the test tube are wiped by alcohol.

Example two

Based on the test tube entering method of the first embodiment, the sample loading device 300 needs to be disposed in the casing 100, so as to receive test tubes one by one and draw the test tubes into the casing 100.

As shown in fig. 5, the loading device 300 includes a receiving mechanism 400 for receiving test tubes and pulling the test tubes toward the gripping mechanism, and a gripping mechanism for gripping the test tubes and sequentially storing the test tubes.

Receiving mechanism 400:

as shown in fig. 6-9, the receiving mechanism 400 includes a mounting bracket 401 located below the sample inlet 202, and a receiving member located between the mounting bracket 401 and the sample inlet 202, where a crank member is mounted on the mounting bracket 401 for driving the receiving member to move between the sample inlet 202 and the gripping member 500, and the receiving member is used for receiving a test tube.

Specifically, as shown in fig. 10 and 11, the crank member includes a second motor 403 and a crank assembly, where the crank assembly is provided with two groups and is respectively located at two sides of the mounting bracket 401.

The crank assembly comprises a swing arm 404 which is hinged on the mounting bracket 401, a hinge shaft formed at the hinge part is horizontally arranged, a traction hole 405 is formed in the swing arm 404 along the extending direction, and the hinge shaft is driven to rotate by a motor II 403.

The mounting bracket 401 is provided with a ring groove 402 along the axial side of the hinge shaft, the ring groove 402 is formed by two groups of horizontal sections and two groups of vertical sections, the connecting part is rounded, the inner and outer contours of the notch are rectangular, a sliding pin 406 is slidably mounted in the ring groove 402, and one end of the sliding pin 406 penetrates through a traction hole 405.

The side surface of the mounting bracket 401 provided with the ring groove 402 is also provided with a first guide rod 407 which is horizontally arranged and vertical to the hinge shaft, a sliding seat is slidably arranged on the first guide rod 407, a second guide rod 408 which is vertically arranged is arranged on the sliding seat, a sliding block 409 is slidably arranged on the second guide rod 408, and the sliding block 409 is movably connected with the sliding pin 406.

The slide block 409 is provided with a slide hole which is vertically arranged, the slide hole is coaxially sleeved with a support rod 410, a limiting ring is arranged outside the support rod 410, the support rod 410 is supported through the cooperation of the limiting ring and the slide block 409, an external step is arranged at the bottom of the support rod 410, and a first spring 411 positioned between the external step and the slide block 409 is sleeved outside the support rod 410.

The working process of the crank member is shown as follows: the second motor 403 drives the hinge shaft to rotate, the hinge shaft drives the swing arm 404 to rotate together, and in the rotating process, the swing arm 404 pulls the sliding pin 406 to move in the ring groove 402 through the pulling hole 405, the sliding pin 406 moves together with the sliding block 409, and the sliding block 409 moves together with the supporting rod 410.

Specifically, as shown in fig. 12 and 13, the receiving member includes a mounting seat 412, a third guide rod 413 is disposed at the bottom of the mounting seat 412, and the third guide rod 413 is parallel to the first guide rod 407.

The push block 414 is installed at the upper end of the support rod 410, the push block 414 is in sliding connection with the guide rod III 413, a push frame 415 is arranged on the outer sliding sleeve of the guide rod III 413, the push frame 415 is located on one side of the push block 414 facing the clamping mechanism, a second spring 416 located between the push frame 415 and the push block 414 is arranged on the outer sleeve of the guide rod III 413, and the free end of the push frame 415 is located on one side of the mounting seat 412 facing the clamping mechanism.

A third spring 417 is disposed between the mounting seat 412 and the pushing frame 415, and when the pushing frame 415 is stationary and the pushing block 414 approaches the clamping mechanism, the elastic force of the third spring 417 drives the mounting seat 412 to approach the clamping mechanism, and in an initial state, the third spring 417 is in a compressed state.

The side of the push block 414 facing the clamping mechanism is also provided with a push rod 418, the push rod 418 is parallel to the guide rod III 413, and the free end of the push rod 418 is positioned between the two ends of the push frame 415.

The upper end face of the mounting seat 412 is provided with a receiving sleeve 419 which is vertically arranged and is provided with an opening at the upper end, and the cavity bottom of the receiving sleeve 419 is provided with a soft cushion.

The operation of the receiving member is represented by: the support bar 410 moves with the receiving member, and when the receiving sleeve 419 is located right under the sample inlet 202, the test tube falling from the sample inlet 202 is received by the receiving sleeve 419, and the receiving member moves with the test tube.

Clamping mechanism:

as shown in fig. 5, the clamping mechanism comprises a fixed bracket 301 arranged in the casing 100 and a rotary ring frame 302 arranged at the upper end of the fixed bracket 301, wherein the rotary ring frame 302 is in an axial vertical circular ring shape and can be driven to rotate by a motor one 303, the outer wall of the rotary ring frame 302 is provided with clamping members 500, and a plurality of groups of clamping members 500 are arranged in an array along the circumferential direction of the rotary ring frame 302; as shown in fig. 6 to 9, the test tube is stored on the corresponding gripping member 500 by the receiving mechanism 400 being engaged with the gripping member 500.

As shown in fig. 14 and 15, the gripping member 500 includes a connection bracket 501 connected to the rotary ring frame 302, a fourth guide rod 502 is disposed on the connection bracket 501 along the radial direction of the rotary ring frame 302, a trigger block 503 is slidably mounted on the outer portion of the fourth guide rod 502, a fourth spring 504 and a fifth spring 505 are respectively disposed on the outer portion of the fourth guide rod 502 in a sleeved manner, and the fourth spring 504 is close to the rotary ring frame 302.

The connecting bracket 501 is also provided with a guide rod five 507 parallel to the guide rod four 502 in a sliding manner, the guide rod five 507 is positioned on one side of the triggering block 503 facing the rotary ring frame 302, one end of the guide rod five 507 facing the triggering block 503 is provided with an abutting step, and the outer part of the guide rod five 507 is sleeved with a spring six 508 positioned between the abutting step and the connecting bracket 501.

The bottom of the connecting bracket 501 is provided with a bottom bracket 506, one end of the bottom bracket 506 is connected with the trigger block 503, and the other end is positioned on one side of the connecting bracket 501 away from the rotary ring frame 302.

As shown in fig. 15 and 16, the upper end surface of the trigger block 503 is provided with trigger grooves, the trigger grooves are symmetrically provided with two groups, and the symmetry axis is parallel to the guide rod four 502.

The groove wall of the trigger groove along the circumferential direction of the rotary ring frame 302 and close to the symmetry axis is composed of a cambered surface I5031, an inclined surface I5032, a transition surface 5033, a plane I5034, a cambered surface II 5035 and a plane II 5036, wherein:

the first cambered surface 5031 is bent towards the direction deviating from the symmetry axis, the first inclined surface 5032 is positioned on one side of the first cambered surface 5031 deviating from the rotary ring frame 302, the distance between the first inclined surfaces 5032 of the two groups of trigger grooves increases gradually along the direction of the symmetry axis and the direction of the fourth spring 504 pointing to the fifth spring 505, the first plane 5034 is parallel to the symmetry axis, the first plane 5034 is positioned on one side of the first inclined surface 5032 deviating from the first cambered surface 5031, and smooth transition connection is realized between the first plane 5034 and the first inclined surface 5032 through the transition surface 5033.

The second plane 5036 is located at one side of the first arc surface 5031 facing the rotating ring frame 302, the second plane 5036 is parallel to the symmetry axis, smooth transition connection is realized between the second plane 5036 and the first arc surface 5031 through the second arc surface 5035, and the second arc surface 5035 bends towards the symmetry axis.

The trigger groove is axially along the symmetry axis and far away from the groove wall of the rotary ring frame 302 and is set as a second inclined plane 5037, the distance between the second inclined plane 5037 and the rotary ring frame 302 increases gradually from bottom to top along the vertical direction, the second inclined plane 5037 can be a surface which is obliquely arranged, and as shown in fig. 16, the trigger groove is formed by a plurality of surfaces which are obliquely arranged, and the oblique direction can be satisfied.

As shown in fig. 14 and 17, the upper end of the connecting bracket 501 is further provided with a clamping bracket 509, the clamping bracket 509 is slidably provided with a clamping arm 510, the extending direction of the clamping arm 510 is parallel to the extending direction of the guide rod four 502, the sliding direction of the clamping arm 510 is horizontally arranged and perpendicular to the extending direction of the guide rod four 502, two groups of clamping arms 510 are arranged along the sliding direction, the clamping bracket 509 is further provided with a spring seven 511 for driving the two groups of clamping arms 510 to move close to each other, and further, the spring seven 511 is provided with two groups and is respectively positioned on one side of the two groups of clamping arms 510 opposite to each other.

The two groups of clamping arms 510 are provided with clamping grooves on one side opposite to the free ends, and the clamping grooves are arc grooves matched with the test tubes and are used for clamping the test tubes.

The bottom of the clamping arm 510 extends downwards to form a sliding sleeve 512, a sliding rod 513 is arranged in the sliding sleeve 512 in a sliding manner, a spring eight 514 is arranged above the sliding rod 513 in the sliding sleeve 512, and in an initial state, the bottom of the sliding rod 513 extends into the trigger groove and is coaxially arranged in the cambered surface one 5031.

As shown in fig. 5, the first guide rod 407 is disposed along the radial direction of the rotating ring frame 302, when the gripping member 500 rotates until the fourth guide rod 502 is parallel to the first guide rod 407 and the gripping member 500 approaches the receiving mechanism 400, the position of the gripping member 500 is named as a gripping position, the test tube is received by the receiving mechanism 400 and is finally gripped by the gripping member 500 in the gripping position, and then the first motor 303 is started to drive the rotating ring frame 302 to rotate, so that the next group of gripping members 500 moves to the gripping position, thereby repeatedly gripping the test tube.

Spring six 508 has a higher spring rate than spring two 416 and a higher spring rate than spring four 504.

The receiving mechanism 400 cooperates with the clamping member 500 located at the clamping position to realize the clamping process of the test tube, which is specifically expressed as follows:

the second motor 403 is operated, the supporting rod 410 is driven to move along the extending direction of the ring groove 402 by the crank member, the supporting rod 410 moves to integrally move with the receiving member, when the receiving sleeve 419 is positioned right below the sample inlet 202, the movement is stopped, the test tube falling from the sample inlet 202 is received by the receiving sleeve 419, and then the supporting rod 410 continues to move with the receiving member and the test tube, and in the moving process:

firstly, the free end of the pushing frame 415 contacts with the bottom frame 506 and pushes the bottom frame 506 to move, the bottom frame 506 moves together with the trigger block 503, the spring IV 504 is compressed, at this time, the trigger block 503 moves to move along the guiding direction of the guide rod IV 502 to approach the rotating ring frame 302, under the inclined guiding of the inclined plane I5032, the sliding rod 513 is switched from the position of the cambered surface I5031 to the position of the plane I5034, the two groups of sliding rods 513 move away from each other, the two groups of clamping arms 510 move away from each other, the spring seven 511 is compressed, the two groups of clamping arms 510 are expanded, at the same time, the sliding rod 513 moves upwards to retract into the sliding sleeve 512 under the guiding of the inclined plane II 5037, and the spring eight 514 is compressed;

then, when the trigger block 503 contacts with the guide rod five 507, since the elastic coefficient of the spring six 508 is greater than that of the spring two 416, the trigger block 503 is motionless, the push frame 415 is motionless, the support bar 410 moves with the push block 414, the spring two 416 is compressed, meanwhile, the spring three 417 releases the elastic force to enable the mounting base 412 and the test tube to continue to move along with the push block 414, when the test tube is located at the position of the clamping groove on the clamping arm 510, the mounting base 412 contacts with the connecting bracket 501, and the push rod 418 contacts with the bottom frame 506;

then, the support rod 410 continues to move with the push block 414, the push block 414 moves with the push rod 418, the push rod 418 directly pushes the underframe 506 to move, the underframe 506 moves with the trigger block 503, the spring six 508 is compressed, the lower end of the slide rod 513 passes through the inclined plane two 5037 and is positioned on the upper end surface of the trigger block 503 under the inclined guidance of the inclined plane two 5037, at the moment, the restriction of the plane one 5034 is avoided, and the spring seven 511 releases the elastic force to drive the two groups of clamping arms 510 to move close to each other so as to clamp the test tube;

then, the support rod 410 continues to move, at this moment, the support rod 410 is located at the corner of the horizontal section and the vertical section, the horizontal movement is switched from horizontal movement to vertical downward movement, in the switching process, the horizontal partial displacement of the support rod 410 continues to move by pushing the trigger block 503 through the push block 414 and the push rod 418, the movement of the trigger block 503 does not interfere with the clamping of the test tube, the vertical partial displacement of the support rod 410 moves downward with the receiving member, then, after the support rod 410 is switched to vertical downward movement, the vertical downward movement of the support rod 410 still moves downward with the receiving member, the receiving sleeve 419 moves downward, the bottom of the test tube is separated from the receiving sleeve 419, meanwhile, the spring two 416 releases the elastic force to reset the receiving member, meanwhile, the spring four 504 releases the elastic force to the spring six 508 to reset the trigger block 503, and in the resetting process, when the slide rod 513 is located right above the cambered surface one 5031, the spring eight 514 releases the elastic force to reset the lower end of the slide rod 513 into the cambered surface one 5031 again, the clamping member 500 resets and clamps the test tube;

finally, the support rod 410 continues to move, and the receiving sleeve 419 is located right below the sample inlet 202 again, at the same time, the first motor 303 operates to drive the rotating ring frame 302 to rotate, so that the next group of clamping members 500 move to the clamping position, and the process is repeated, so that the next test tube is received and clamped.

In the above process, the following actions can be realized by driving the second motor 403: the receiving sleeve 419 receives the test tube, the clamping arms 510 are opened, the test tube is pulled to move between the two groups of clamping arms 510, the clamping arms 510 are closed to clamp the test tube, the test tube is separated from the receiving sleeve 419, the five transfer actions form a complete clamping action of the test tube, the action and the action are connected smoothly without any pause, the efficiency is high, and the driving mode of a single power source is energy-saving.

Further, as shown in fig. 5, after the sample in the test tube is used, the test tube needs to be discarded, and the clamping member 500 is emptied, so that a discarding hole is further provided at the bottom of the casing 100, when the sample in the test tube is used, the first motor 303 operates the clamping member 500 corresponding to the sample in the test tube to move to the position where the discarding hole is located, so that the test tube is coaxial with the discarding hole, then the telescopic rod 304 radially arranged on the fixed bracket 301 along the rotating ring frame 302 pushes the trigger block 503 to move away from the rotating ring frame 302, and in the moving process, under the guidance of the second cambered surface 5035, the lower end of the sliding rod 513 passes over the second cambered surface 5035 and moves to the second cambered surface 5036, and simultaneously, the two groups of sliding rods 513 move away from each other, so that the two groups of clamping arms 510 move away from each other together, the clamping arms 510 release the clamping of the test tube, the test tube drops down, and the waste box can be arranged below the discarding hole to receive the discarded test tube; the telescopic rod 304 can be realized by the existing electric telescopic rod technical means.

Further, as shown in fig. 12, a two-dimensional code is generally disposed outside the test tube and used for being scanned and identified by the scanner to number the test tube, so as to avoid management confusion of sample detection results in different test tubes, therefore, the receiving sleeve 419 is rotatably mounted on the mounting seat 412, and the mounting seat 412 is further provided with a motor III 700 for driving the receiving sleeve 419 to rotate, so that the two-dimensional code on the test tube is rotated to be opposite to the scanner, and the auxiliary scanner records the numbers of the test tubes.

Example III

As shown in fig. 18 to 23, in the immunoassay process, the probe for sucking the sample needs to be cleaned once after being used once, and based on this, the invention is provided with a cleaning mechanism 600, specifically:

the cleaning mechanism 600 includes a cleaning frame and a sampling needle 601 mounted on the cleaning frame.

As shown in fig. 20, the sampling needle 601 includes an outer mounting sleeve 6011 which is arranged vertically and is arranged on a cleaning frame, the upper end of the outer mounting sleeve 6011 is sealed, a sampling tube 6012 is mounted inside the outer mounting sleeve 6011 through an inner bracket, the sampling tube 6012 is coaxial with the outer mounting sleeve 6011, the lower end of the sampling tube 6012 extends out of the outer mounting sleeve 6011, a liquid level detection needle 6013 is further coaxially arranged in the sampling tube 6012, the upper end of the liquid level detection needle 6013 extends out of the outer mounting sleeve 6011 and is driven to move up and down by a linear module 602 arranged on the cleaning frame, in an initial state, the lower end of the liquid level detection needle 6013 and the lower end of the outer mounting sleeve 6011 are located on the same horizontal plane, the liquid level detection needle 6013 adopts the existing liquid level detection following technology to monitor the liquid level in the sampling tube 6012, the sampling tube 6012 is used for quantitatively sampling samples or reagents, and the liquid level detection needle 6013 detects the liquid level of a sampling result, and further analyzes and detects the accuracy of the quantitative sampling result.

The linear module 602 may be an electric telescopic rod technology or a linear screw rod technology, and in addition, the movement of the sampling needle 601 between the sample, the reagent, the incubation reaction tube and the waste liquid collecting tube may be realized in the prior art, for example, the structure in the existing immunoassay device may be referred to or the three-dimensional moving structure in the 3D printer may be referred to, which is not described in detail.

The outer circular surface of the outer mounting sleeve 6011 is provided with a joint 6015, the upper end of the liquid level detection needle 6013 is opened, the lower end is closed, the outer circular surface is provided with a water outlet hole 6014, and the water outlet hole 6014 is close to the lower end of the liquid level detection needle 6013 and is provided with a plurality of water outlet holes along the circumferential direction array of the liquid level detection needle 6013.

As shown in fig. 19, the cleaning mechanism 600 further includes a switching valve 603.

As shown in fig. 21 to 23, the switching valve 603 includes a valve housing 6031, the valve housing 6031 has a rectangular shape with an inner circumference and an outer circumference, four sides of the valve housing 6031 are respectively provided with a nipple 6032, a cavity wall of the valve housing 6031 is provided with a side single hole 6034 and a side double groove 6033, the side single hole 6034 is communicated with the nipple 6032, and the side double groove 6033 is provided with three and is respectively communicated with the remaining three nipples 6032 in one-to-one correspondence.

An outer valve sleeve 6035 is coaxially rotatably arranged in the valve housing 6031, a mounting groove in the shape of a circular groove is coaxially arranged on the lower end face of the outer valve sleeve 6035, an inner valve core 6037 is coaxially arranged in the mounting groove, and the bottom of the inner valve core 6037 extends out of the valve housing 6031.

The inner valve core 6037 is axially divided into an upper valve section and a lower valve section which are coaxial and unequal in diameter, the outer diameter of the upper valve section is smaller than the outer diameter of the lower valve section, and the mounting groove is axially divided into an upper groove section and a lower groove section which are coaxial and unequal in diameter, and the inner diameter of the upper groove section is smaller than the inner diameter of the lower groove section.

The upper end of the upper valve section, the shaft shoulder of the upper valve section and the lower valve section and the outer circular surface of the lower valve section are all extended to be provided with mounting steps, the inner valve core 6037 is mounted in the mounting groove through the cooperation of the mounting steps and the mounting groove, and the upper groove section and the lower groove section of the mounting groove are separated and are not communicated with each other.

The outer surface of the outer valve sleeve 6035 is radially provided with an outer valve hole 6036, the outer valve hole 6036 is provided with two groups of upper outer valve holes communicated with the upper groove section and lower outer valve holes communicated with the lower groove section, the bottom of the inner valve core 6037 is provided with an inner valve hole 6038, and the inner valve hole 6038 is provided with two groups of inner valve holes communicated with the upper groove section and inner valve holes communicated with the lower groove section.

As shown in fig. 21, the free end of the connector 6032 is provided with four pipes, which are respectively: the end is connected with a water source pipe of a cleaning medium source (such as water), the end is connected with a hot air pipe of a hot air source, the end is connected with a cold air pipe of a cold air source, and the end is connected with an exhaust pipe of an exhaust pump 604, wherein a joint 6032 communicated with the exhaust pipe is communicated with a side single hole 6034.

A first connecting pipe 605 is arranged between the first inner valve hole and the joint 6015, a second connecting pipe 606 is arranged between the second inner valve hole and the liquid level detection needle 6013, and a fourth motor 607 for driving the outer valve sleeve 6035 to rotate is arranged on the valve housing 6031.

Working procedure of example three:

the sampling needle 601 extends into the sample or reagent, and at the same time, the motor IV 607 drives the outer valve sleeve 6035 to rotate, so that the side single hole 6034 is communicated with the lower outer valve hole, and the upper outer valve hole is blocked by the cavity wall of the valve housing 6031;

then, the air pump 604 operates, air in the sampling tube 6012 is extracted through the air extraction tube, the connector 6032, the side single hole 6034, the lower outer valve hole, the lower groove section, the inner valve hole II, the connecting tube II 606 and the water outlet 6014, under the negative pressure effect, a sample or a reagent is accurately extracted into the sampling tube 6012, meanwhile, the linear module 602 drives the liquid level detection needle 6013 to move upwards, the liquid level in the sampling tube 6012 is detected through the liquid level detection needle 6013, the amount of the sample or the reagent in the sampling tube 6012 can be calculated through the detected liquid level height, the purpose of further analyzing and detecting the accuracy of quantitative sampling results is achieved, and the accuracy of an immunoassay process is further improved;

after the sampling is finished, the sampling needle 601 pulls the sampled sample or reagent into the detection tube, and incubation reaction detection is performed.

The sampling needle 601 needs to be cleaned after sampling once, specifically:

firstly, a motor IV 607 drives an outer valve sleeve 6035 to rotate, so that a side double-groove 6033 corresponding to a connector 6032 communicated with a water source pipe is simultaneously communicated with an upper outer valve hole and a lower outer valve hole, a cleaning medium provided by a cleaning medium source sequentially passes through the water source pipe and the connector 6032 to enter the side double-groove 6033, then the cleaning medium is divided into two parts, one part of the cleaning medium sequentially passes through the upper outer valve hole, an upper groove section, an inner valve hole I, a connecting pipe I605 and a connector 6015 to flow into an outer mounting sleeve 6011 and downwards through the outer mounting sleeve 6011, the outer wall of the sampling pipe 6012 is washed and cleaned, the other part of the cleaning medium sequentially passes through a lower outer valve hole, a lower groove section, an inner valve hole II, a connecting pipe II 606 and a water outlet 6014 to flow into the sampling pipe 6012, the cleaning medium flows in a vortex manner in the sampling pipe 6012 under the inclined guide of the water outlet 6014, and the cleaning medium is further arranged into a cleaning arrangement, and the cleaning effect is better;

then, the motor IV 607 drives the outer valve sleeve 6035 to rotate, so that the side double grooves 6033 corresponding to the connecting mouth 6032 communicated with the hot air pipe are simultaneously communicated with the upper outer valve hole and the lower outer valve hole, hot air provided by the hot air source is divided into two parts, and is blown towards the outer wall and the inner wall of the sampling pipe 6012 respectively, and after cleaning, cleaning mediums remained on the outer wall and the inner wall of the sampling pipe 6012 are evaporated, namely the sampling pipe 6012 is dried, so that the residual cleaning mediums do not interfere with the concentration and the amount of reagents sampled next time;

then, the motor four 607 drives the outer valve sleeve 6035 to rotate, so that the side double grooves 6033 corresponding to the connector 6032 communicated with the cold air pipe are simultaneously communicated with the upper outer valve hole and the lower outer valve hole, cold air provided by the cold air source is divided into two parts, and the cold air is blown towards the outer wall and the inner wall of the sampling pipe 6012 respectively to cool the sampling pipe 6012, so that the influence such as damage to a sample or a reagent to be sampled next caused by the high temperature of the dried surface of the sampling pipe 6012 is avoided.

In the above-mentioned cleaning process, can realize respectively carrying out negative pressure extraction, clean, dry, four actions of cooling to sampling needle 601 through a change-over valve 603, its meaning lies in, among the prior art, and what the skilled person thinks easily is through a plurality of solenoid valves and many pipeline cooperation, realizes above-mentioned same effect, but: on one hand, control switching of the plurality of electromagnetic valves is realized through a preset program, the preset program is complex and tedious, the opening and closing of the electromagnetic valves are delayed, cleaning efficiency is affected, and then detection efficiency is affected; in contrast, according to the invention, the sampling needle 601 can be subjected to four actions of negative pressure extraction, cleaning, drying and cooling through a group of switching valves 603, no redundant flow track exists, and the cleaning efficiency is higher.

The present invention is not limited to the above embodiments, but is capable of modification and variation in detail, and other modifications and variations can be made by those skilled in the art without departing from the scope of the present invention.

Claims

1. An immunoassay equipment for tumor screening, includes the casing, its characterized in that: the shell is provided with a sample injection mechanism, the sample injection mechanism comprises a mounting table arranged on the side face of the shell, the mounting table is provided with a sample injection port, the side face of the shell is provided with a sample injection hole, the lower orifice of the sample injection port is attached to the upper orifice of the sample injection hole, the sample injection mechanism further comprises a synchronous belt arranged on the mounting table, the synchronous belt is provided with two groups of sample injection ports and is positioned between the two groups of synchronous belts, the outer surface of the synchronous belt is provided with a traction groove in a shape of an arc groove in a penetrating way, and the traction grooves are arranged in a plurality along the extending direction of the synchronous belt in an array way;

the opposite side surfaces of the two groups of synchronous belts are attached, and the diameter of the traction groove is consistent with the diameter of the test tube;

the machine shell is internally provided with a sample adding device, the sample adding device comprises a receiving mechanism and a clamping mechanism, the clamping mechanism comprises a fixed support arranged in the machine shell and a rotary ring frame arranged at the upper end of the fixed support, the outer wall of the rotary ring frame is provided with clamping members, the clamping members are provided with a plurality of groups along the circumferential direction of the rotary ring frame in an array manner, and the receiving mechanism is matched with the clamping members to receive and clamp test tubes;

the receiving mechanism comprises a mounting bracket positioned below the sample inlet and a receiving member positioned between the mounting bracket and the sample inlet, wherein a crank member is arranged on the mounting bracket and comprises a motor II and a crank assembly, and the crank assembly is provided with two groups and is respectively positioned at two sides of the mounting bracket;

the crank assembly comprises a swing arm which is hinged on the mounting bracket, and a traction hole is formed in the swing arm along the extending direction;

the side surface of the mounting bracket is provided with a ring groove, the inner contour and the outer contour of the notch of the ring groove are rectangular, a sliding pin is slidably arranged in the ring groove, and one end of the sliding pin penetrates through the traction hole;

the side surface of the mounting bracket, provided with the annular groove, is also provided with a guide rod I which is horizontally arranged, a slide seat is slidably arranged on the guide rod I, a guide rod II which is vertically arranged is arranged on the slide seat, a slide block is slidably arranged on the guide rod II, the slide block is movably connected with the slide pin, a slide hole which is vertically arranged is formed in the slide block, and a support rod is arranged in the slide hole;

the receiving component comprises a mounting seat, a guide rod III parallel to the guide rod I is arranged at the bottom of the mounting seat, a push block is arranged at the upper end of the support rod and is in sliding connection with the guide rod III, a push frame is sleeved outside the guide rod III in a sliding manner, the push frame is positioned at one side of the push block facing the clamping mechanism, a spring II positioned between the push frame and the push block is sleeved outside the guide rod III, the free end of the push frame is positioned at one side of the mounting seat facing the clamping mechanism, a spring III is arranged between the mounting seat and the push frame, and in an initial state, the spring III is in a compression state;

the side surface of the push block facing the clamping mechanism is also provided with a push rod, the push rod is parallel to the guide rod III, the free end of the push rod is positioned between the two ends of the push frame, and the upper end surface of the mounting seat is provided with a receiving sleeve which is vertically arranged and is provided with an opening at the upper end;

the clamping component comprises a connecting bracket connected with the rotary ring frame, a guide rod IV is arranged on the connecting bracket along the radial direction of the rotary ring frame, a trigger block is slidably arranged outside the guide rod IV, a spring IV and a spring V which are respectively positioned at two sides of the trigger block are sleeved outside the guide rod IV, the spring IV is close to the rotary ring frame, and the elastic coefficient of the spring VI is larger than that of the spring II and is larger than that of the spring IV;

the connecting support is also provided with a guide rod five which is parallel to the guide rod four in a sliding manner, the guide rod five is positioned on one side of the triggering block, which faces the rotating ring frame, one end of the guide rod five, which faces the triggering block, is provided with a collision step, the outer part of the guide rod five is sleeved with a spring six positioned between the collision step and the connecting support, the bottom of the connecting support is provided with a bottom frame, one end of the bottom frame is connected with the triggering block, and the other end of the bottom frame is positioned on one side of the connecting support, which is away from the rotating ring frame;

the upper end surface of the trigger block is provided with trigger grooves which are symmetrically provided with two groups, and the symmetry axis is parallel to the guide rod IV;

the trigger groove is formed by a first cambered surface, a first inclined surface, a transition surface, a first plane, a second cambered surface and a second plane along the circumferential direction of the rotary ring frame and close to the symmetry axis, the first cambered surface is bent towards the direction deviating from the symmetry axis, the first inclined surface is positioned at one side of the first cambered surface deviating from the rotary ring frame, the distance between the inclined surfaces of the two groups of trigger grooves increases gradually along the axial direction of the symmetry axis and along the direction of the spring four to the spring five, the first plane is parallel to the symmetry axis and is positioned at one side of the first inclined surface deviating from the cambered surface, the first plane and the first inclined surface are connected through the transition surface, the second plane is positioned at one side of the cambered surface facing the rotary ring frame, the second plane is parallel to the symmetry axis, the second plane and the first cambered surface are connected through the second cambered surface, and the second cambered surface is bent towards the symmetry axis;

the trigger groove is axially arranged along the symmetry axis and far away from the groove wall of the rotary ring frame to form a second inclined plane, and the distance between the second inclined plane and the rotary ring frame is gradually increased from bottom to top along the vertical direction;

the upper end of the connecting bracket is also provided with a clamping bracket, the clamping bracket is provided with clamping arms in a sliding manner, the extending direction of the clamping arms is parallel to the extending direction of the guide rod IV, the sliding direction of the clamping arms is horizontally arranged and perpendicular to the extending direction of the guide rod IV, the clamping arms are provided with two groups along the sliding direction, and the clamping bracket is also provided with two groups of springs seven which are respectively positioned on one sides of the two groups of clamping arms opposite to each other;

the bottom of the clamping arm vertically extends downwards to form a sliding sleeve, a sliding rod is arranged in the sliding sleeve in a sliding mode, a spring eight positioned above the sliding rod is further arranged in the sliding sleeve, and in an initial state, the bottom of the sliding rod stretches into the trigger groove and is coaxially positioned in the first cambered surface.

2. The immunoassay device for tumor screening according to claim 1, wherein: the shell is internally provided with a cleaning mechanism, the cleaning mechanism comprises a sampling needle, the sampling needle comprises an outer mounting sleeve, the upper end of the outer mounting sleeve is closed, a sampling tube is mounted inside the sampling needle through an inner support, the sampling tube is coaxial with the outer mounting sleeve, the lower end of the sampling tube extends out of the outer mounting sleeve, a liquid level probe is coaxially arranged in the sampling tube, the upper end of the liquid level probe extends out of the outer mounting sleeve and is arranged in a lifting manner, and in an initial state, the lower end of the liquid level probe and the lower end of the outer mounting sleeve are positioned on the same horizontal plane;

the outer circle surface of the outer installation sleeve is provided with a connector, the upper end opening and the lower end of the liquid level probe are closed, the outer circle surface is provided with a water outlet hole, and the water outlet hole is close to the lower end of the liquid level probe and is provided with a plurality of water outlet holes along the circumferential direction array of the liquid level probe.

3. The immunoassay device for tumor screening according to claim 2, wherein: the cleaning mechanism also comprises a switching valve, an air pump, a cleaning medium source, a hot air source and a cold air source;

the switching valve is arranged in the pumping state, the cleaning state, the drying state and the cooling state, the switching valve is used for communicating the sucking pump with the liquid level probe when the pumping state is adopted, the switching valve is used for communicating the cleaning medium source with the connector and the liquid level probe when the cleaning state is adopted, the switching valve is used for communicating the hot air source with the connector and the liquid level probe when the cooling state is adopted, and the switching valve is used for communicating the cold air source with the connector and the liquid level probe when the cooling state is adopted.