CN116396854A - Biochip reader capable of rapidly detecting sterilization effect - Google Patents
Biochip reader capable of rapidly detecting sterilization effect Download PDFInfo
- Publication number
- CN116396854A CN116396854A CN202310401476.5A CN202310401476A CN116396854A CN 116396854 A CN116396854 A CN 116396854A CN 202310401476 A CN202310401476 A CN 202310401476A CN 116396854 A CN116396854 A CN 116396854A
- Authority
- CN
- China
- Prior art keywords
- chip
- reader
- reading
- key
- intelligent controller
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 116
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 95
- 230000000694 effects Effects 0.000 title claims abstract description 78
- 238000000018 DNA microarray Methods 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 12
- 241000233866 Fungi Species 0.000 claims abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims description 33
- 238000005192 partition Methods 0.000 claims description 27
- 230000010365 information processing Effects 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 19
- 238000007789 sealing Methods 0.000 claims description 13
- 230000003287 optical effect Effects 0.000 claims description 11
- 238000012790 confirmation Methods 0.000 claims description 9
- 230000005856 abnormality Effects 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 238000012544 monitoring process Methods 0.000 claims description 7
- 239000004966 Carbon aerogel Substances 0.000 claims description 6
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 6
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 claims description 6
- 239000010703 silicon Substances 0.000 claims description 6
- 229910052710 silicon Inorganic materials 0.000 claims description 6
- 229910052726 zirconium Inorganic materials 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 229920000049 Carbon (fiber) Polymers 0.000 claims description 3
- 239000004917 carbon fiber Substances 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 3
- 229910021389 graphene Inorganic materials 0.000 claims description 3
- 239000004973 liquid crystal related substance Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 238000004092 self-diagnosis Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 43
- 238000011156 evaluation Methods 0.000 abstract description 8
- 238000011161 development Methods 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 14
- 238000011534 incubation Methods 0.000 description 9
- 229910052736 halogen Inorganic materials 0.000 description 4
- 150000002367 halogens Chemical class 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 229910052724 xenon Inorganic materials 0.000 description 4
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical group [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical group [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 3
- 229910052753 mercury Inorganic materials 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012854 evaluation process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K7/00—Methods or arrangements for sensing record carriers, e.g. for reading patterns
- G06K7/10—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation
- G06K7/14—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation using light without selection of wavelength, e.g. sensing reflected white light
- G06K7/1404—Methods for optical code recognition
- G06K7/1408—Methods for optical code recognition the method being specifically adapted for the type of code
- G06K7/1413—1D bar codes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Thermal Sciences (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Artificial Intelligence (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Electromagnetism (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Theoretical Computer Science (AREA)
- Computer Hardware Design (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a biochip reader for rapidly detecting sterilization effect, which not only solves the problem of rapid sterilization effect detection of a plurality of fungus cavity chips and a large number of chips, but also ensures that an operation light source in the whole biochip reading process is selected to be intelligent, dynamic data of fluorescence detection is obtained in real time and data are stored in a database, and provides powerful guarantee for promoting the efficient development of laboratory sterilization effect evaluation work of a rapid detection chip for sterilization effect after sterilization.
Description
Technical Field
The invention belongs to the technical field of biochip monitoring, and particularly relates to a biochip reader for rapidly detecting a sterilization effect.
Background
With the advancement of medical technology, a large number of medical instruments are used in clinical testing and medicine. In order to reduce or avoid medical disputes caused by incomplete disinfection and sterilization, and detection results of ' false negative ' and ' false positive ', national standards of national people's republic of China such as GB/T15981-2021 disinfection instrument disinfection effect evaluation methods are provided for guiding and standardizing medical instrument disinfection effect evaluation. However, due to limitations of the evaluation technique, the conventional evaluation method of the sterilization effect cannot clearly determine whether the sterilization effect is qualified or not and whether the national requirements are met or not. Although the above problems are well solved by the development of a chip for rapid detection of sterilization effect (a chip for rapid detection of sterilization effect, chinese patent application No. 2023103563182) which is compatible with the culture holes of existing biological readers and can realize "sterilization completion", the inquiry of related patents and documents is not performed, and no matched biological chip reader for domestic scholars to study the rapid detection of sterilization effect is found. Meanwhile, the existing biological reader compatible with the chip can only read the capability of a single bacteria tablet cavity chip placed in a single culture hole, but cannot read a plurality of bacteria tablet cavity chips. In addition, the low-end configured fluorescence microscope affects the degree of intelligence in biochip reading, while the high-end configured fluorescence microscope greatly increases the cost of biochip reading.
Therefore, it is very necessary to develop a reader which is suitable in cost and can realize that the sterilization is finished, and the sterilization effect is detected and read by a chip quickly, so that the quick determination of the sterilization effect of the medical instrument each time is realized, the integration and the automation degree of the chip sterilization effect detection are improved, and meanwhile, the problems of false negative and false positive in the sterilization effect evaluation process are solved.
Disclosure of Invention
The invention aims to overcome the defects of the prior reading technology of a medical instrument sterilization effect rapid detection chip, provides a biochip reader for detecting the rapid sterilization effect, can overcome the problems or partially solve the problems, and provides powerful guarantee and cost reduction for effectively developing laboratory sterilization effect evaluation work of the sterilization effect rapid detection chip after sterilization is finished.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
the biochip reader comprises a chip reading cabin and a cabin door connected with the chip reading cabin through a hinge, wherein the chip reading cabin comprises a chip reading platform, a light source, an optical transceiver and a fluorescence detector, the reading platform, the light source, the optical transceiver and the fluorescence detector are respectively and electrically connected with an intelligent controller through connecting wires, and the intelligent controller is electrically connected with an external power supply and a reserve power supply.
Preferably, the chip reading cabin is composed of a first partition board, a second partition board and a third partition board which are parallel from top to bottom.
Preferably, a panel of the intelligent controller is arranged on the first partition board parallel to the chip reading cabin from top to bottom.
Preferably, an intelligent controller is arranged below the first partition plates parallel to the chip reading cabin from top to bottom.
Preferably, a reserve power supply and an external power line storage box are arranged on the second partition plate parallel to the chip reading cabin from top to bottom.
Preferably, a light source and a light source system switching controller are arranged below a second partition plate parallel to the chip reading cabin from top to bottom, and the light source system switching controller are electrically connected with a reserve power supply and an external power supply which are arranged on the second partition plate.
Preferably, the second partition plates parallel to the chip reading cabin from top to bottom are made of a mixed material of graphene and zirconium boride, and the mass content of the zirconium boride in the mixed material is 2% -12%.
Preferably, the light source and the light source system switching controller are electrically connected with an intelligent controller through a connecting wire, and the intelligent controller is electrically connected with an external power supply and a reserve power supply.
In one preferred embodiment, the chip reading cabin is provided with 1-5 kinds of light sources, and the light sources are matched with corresponding light source systems. In one preferred embodiment, the light source of the chip reader is a xenon lamp, and the light source system of the xenon lamp comprises a light filtering device. In some embodiments, the light source of the chip reader is a mercury lamp, and the light source system of the mercury lamp comprises an excitation filter adding system. In some embodiments, the light source of the chip reader is a metal halogen lamp, and the light source system of the metal halogen lamp comprises a built-in intensity design device. In some embodiments, the light source of the chip reader is an LED white light fluorescent light source, and the light source system of the LED white light fluorescent light source includes a filter, an external controller, and SDK software. In some embodiments, the light source of the chip reader is an LED single-wavelength fluorescent light source, and the light source system of the LED single-wavelength fluorescent light source includes a program controller.
In one preferred embodiment, the chip reading cabin is provided with 2-5 kinds of light sources in the above embodiment, the selection of the kinds of the light sources is intelligently matched through the intelligent controller, and the light source system switching controller is used for matching the light source system of the selected light source.
Preferably, a chip reading platform, an optical transceiver, a fluorescence detector and a cabin door switch state sensor are arranged on a third partition plate which is parallel from top to bottom of the chip reading cabin; the chip reading platform is provided with a chip placement sensor, a chip temperature sensor, a chip placement abnormality or culture abnormality alarm device and a heating film; the chip reading platform, the optical transceiver and the fluorescence detector cabin door switch state sensor are all electrically connected with the intelligent controller through connecting wires.
In one preferred embodiment, the chip reading platform can place 1-50 chips. In one embodiment, the number of chips that the chip reading platform can place is 2. In some embodiments, the number of chips that the chip reading platform can place is 3-10, respectively. In some embodiments, the number of chips that the chip reading platform can place is 11-20, respectively. In some embodiments, the number of chips that the chip reading platform can place is 21-30, respectively. In some embodiments, the number of chips that the chip reading platform can place is 31-40, respectively. In some embodiments, the number of chips that the chip reading platform can place is 41-49, respectively.
Preferably, the intelligent controller performs intelligent control through an intelligent control system, the intelligent control system comprises a micro server, a sensing information processing module placed in a chip of a chip reading platform, a sensing information processing module of the temperature of the chip reading platform, an alarm information processing module of a chip reading platform, a sensing information processing module of a cabin door switch state, an information processing module of a light source, an information processing module of an optical transceiver and an information processing module of a fluorescence detector, and the micro server comprises a processor and a database, and the sensing information processing module, the alarm information processing module and the information processing module are electrically connected with the processor.
Preferably, the cabin body is made of a mixed material of carbon fibers and silicon boride, wherein the third partition plate is parallel from top to bottom, and the mass content of the silicon boride in the mixed material is 23% -38%.
Preferably, the reserve power supply can be used for supplying power in the case of power failure and no external power supply.
Preferably, the panel of the intelligent controller comprises a bar code reading head, a liquid crystal display screen, a reader function key, a chip fluorescence detection dynamic data monitoring wireless transmission key, an equipment log key, a fault self-diagnosis key, an equipment remote control key and an equipment start/stop key.
Preferably, the reader function keys of the intelligent controller comprise a chip placement confirmation key, a chip bar code scanning key, a bacterial chip sample bar code scanning key, a cabin door switch state display key, a chip reading temperature compensation function key and a light source intelligent selection key.
Preferably, the chip fluorescence detection dynamic data monitoring wireless transmission key of the intelligent controller comprises a chip fungus sheet cavity fluorescence intensity-time curve recording function key and a chip fungus sheet cavity fluorescence detection image-time recording key.
Further, the chip reading platform is provided with a card seat for fixing the chip.
Preferably, the material of the chip reading platform is porous carbon aerogel material, and the pore diameter of the porous carbon aerogel is between 0.1 and 0.5 micron.
The application method of the biochip reader for rapidly detecting the sterilization effect comprises the following steps: firstly, switching on a power supply, registering a bar code on a chip body and a bar code of a bacterial sample by a bar code reading head on an intelligent controller panel, and pressing a confirmation key on the intelligent controller panel to confirm; after the code scanning registration of the chip is completed, opening a cabin door sealing ring and a cabin door of the chip reader in sequence, and placing the chip to a reading platform of the chip reader to be fixed through a corresponding clamping seat; step three, confirming the position feedback information of the chip in the chip reader through the intelligent controller panel; step four, after the position information of the placed chip in the chip reader is confirmed to be correct, loading and placing the next chip according to the flow from the step one to the step three; resetting a chip reader cabin door and a cabin door sealing ring after all chips to be loaded are placed in the reader; step six, a plurality of fine item parameters such as reading temperature, reading times, reading time and the like are input through an intelligent controller panel, and the parameters can be increased or decreased by pressing upper and lower keys in an operation page during setting. If the modified parameters are to be saved, the confirmation key in the operation page is pressed, the heating film can be started to heat so as to reach the reading temperature required by the chip. Before reading, the chip is first matched with proper light source through fluorescent scanning to the chip and then read. The chip fluorescence detection dynamic data are transmitted to the appointed object in a wireless way through the intelligent controller of the chip reader during reading, and the corresponding data are synchronously stored in the database of the intelligent controller; step seven, after reading, turning off the power supply, opening the cabin door sealing ring, the cabin door and the chip reading platform clamping seat in turn according to actual conditions, taking out the corresponding chips for recycling treatment, and resetting the chip reading platform clamping seat, the cabin door and the cabin door sealing ring in turn; and step eight, the external power line is accommodated in the external power line accommodating box for standby.
After the technical scheme is adopted, compared with the prior art, the invention has the following beneficial effects:
(1) The invention fills the blank of a biochip reader for rapidly detecting the sterilization effect, solves the problem for rapidly detecting the sterilization effect of a plurality of fungus cavity chips and a large number of chips, and provides powerful guarantee for promoting the use of the high-efficiency development of the laboratory sterilization effect evaluation work of rapidly detecting the chip of the sterilization effect after the sterilization is finished;
(2) The invention provides an intelligent control system of a biochip reader for rapidly detecting sterilization effect, which ensures that the operation light source selection in the whole biochip reading process is intelligent, the dynamic data acquisition of fluorescence detection is real-time and the data storage is database, thereby greatly simplifying the work flow of inspection staff.
Drawings
Fig. 1 is a front view of a biochip reader according to example 1 for rapidly detecting sterilization effects.
Fig. 2 is a sectional view of a biochip reader for rapidly detecting sterilization effects according to example 1.
Fig. 3 is a functional schematic diagram of the intelligent control panel of the biochip reader according to example 1 for rapidly detecting sterilization effect.
Description of the embodiments
The invention will be described in detail below with reference to the drawings in connection with embodiments. It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other. For convenience of description, the words "upper", "lower", "left" and "right" are used hereinafter to denote only the directions corresponding to the upper, lower, left, and right directions of the drawings, and do not limit the structure. The examples of the present invention are merely for the purpose of explaining the present invention and are not intended to limit the present invention, and the examples of the present invention are not limited to the examples given in the specification. The specific experimental or operating conditions were not noted in the examples and were made under conventional conditions or under conditions recommended by the material suppliers. Furthermore, it is to be understood that the reference to one or more method steps in this disclosure does not exclude the presence of other method steps before or after the combination step or the insertion of other method steps between these explicitly mentioned steps, unless otherwise indicated; it is also to be understood that the use of a combination connection in accordance with the invention does not exclude the presence of other connections before or after the combination or the insertion of other connections between the two explicitly mentioned connections, unless otherwise indicated. Moreover, unless otherwise indicated, the numbering of the method steps is merely a convenient tool for identifying the method steps and is not intended to limit the order of arrangement of the method steps or to limit the scope of the invention in which the invention may be practiced, as such changes or modifications in their relative relationships may be regarded as within the scope of the invention without substantial modification to the technical matter. In the examples described below, reagents, materials and apparatus used are commercially available unless otherwise specified.
The chip reading cabin body, the cabin door, the first partition board, the second partition board, the third partition board, the intelligent controller panel, the intelligent controller, the reserve power supply, the external power line storage box, the chip reading platform, the light source, the optical transceiver, the fluorescence detector, the chip placement sensor, the chip temperature sensor, the chip placement abnormality or culture abnormality alarm device, the heating film, the cabin door switch state sensor and the cabin door handle component are all universal standard components or components known by a person skilled in the art, and the structure and the principle of the device are all known by the person skilled in the art through technical manuals or through a routine experiment method.
Example 1A biochip reader for rapidly detecting sterilizing Effect
A biochip reader for rapidly detecting sterilization effect is mainly composed of a chip reading cabin body 1 and a cabin door 2 hinged on the cabin body, wherein the cabin body 1 is composed of a first partition board 3, a second partition board 4 and a third partition board 5 which are parallel from top to bottom, as shown in figures 1 and 2. An intelligent controller panel 6 is installed on the first partition plate 3, and an intelligent controller 7 is installed below the first partition plate 3. A reserve power supply 8 and an external power line storage box 9 are arranged on the second partition board, and a light source 10 and a light source system switching controller 11 are arranged below the second partition board;
the third partition board 5 is provided with a chip reading platform 12, an optical transceiver 13, a fluorescence detector 14 and a cabin door switch state sensor 15. The chip reading platform 12, the optical transceiver 13, the fluorescence detector 14 and the cabin door switch state sensor 15 are all electrically connected with the intelligent controller 7 through connecting wires. The chip reading platform 12 is provided with a chip placement sensor 16, a chip temperature sensor 17, a chip placement abnormality or culture abnormality alarm device 18 and a heating film 19. Furthermore, the hatch 2 is provided with a hatch sealing ring 20, a handle 21 and a hinge 22 connected to the hatch 1;
in example 1, the second separator was made of a mixed material of graphene and zirconium boride, in which the mass content of zirconium boride was 8%. The method comprises the steps of carrying out a first treatment on the surface of the
In the embodiment 1, the cabin body is made of a mixed material of carbon fiber and silicon boride, wherein the mixed material is parallel to the third separator from top to bottom, and the mass content of the silicon boride in the mixed material is 30%;
in embodiment 1, the material of the chip reading platform 12 is a porous carbon aerogel material, and the pore diameter of the porous carbon aerogel is between 0.3 micrometers;
in embodiment 1, if the light source 10 is a xenon lamp, the light source system switching controller 11 matches a xenon lamp light source system including a double filter-turning structure, a 1ST filter device, and a 2ND filter device;
in embodiment 1, if the light source 10 is a mercury lamp, the light source system switching controller 11 is matched with a tribute lamp light source system comprising an excitation filter adding system;
in embodiment 1, if the light source 10 is a metal halogen lamp, the light source system switching controller 11 is matched with a metal halogen lamp light source system including a built-in intensity design device;
in embodiment 1, if the light source 10 is an LED white light fluorescent light source, the light source system switching controller 11 is matched with an LED white light fluorescent light source system including a filter, an external controller and SDK software;
in embodiment 1, if the light source 10 is an LED single-wavelength fluorescent light source, the light source system switching controller 11 matches an LED single-wavelength fluorescent light source system including a program controller;
in embodiment 1, as shown in fig. 3, the intelligent controller intelligent control panel 6 includes a barcode reading head 23, a liquid crystal display 24, a reader function key 25, a chip fluorescence detection dynamic data monitoring wireless transmission key 26, a device log key 27, a fault self-diagnosis key 28, a device remote control key 29 and a device start/stop key 30;
the reader function key 25 comprises a chip placement confirmation key 31, a chip bar code scanning key 32, a bacterial sheet sample bar code scanning key 33, a cabin door switch state display key 34, a chip reading temperature compensation function key 35 and a light source intelligent selection key 36;
the chip temperature dynamic data monitoring wireless transfer key 26 comprises a chip temperature-time curve recording function key 37 and a chip temperature-voltage-current-efficiency curve recording key 38.
Example 2 example of Using a biochip reader to rapidly detect sterilizing Effect
The application method of the biochip reader for rapidly detecting the sterilization effect comprises the following steps: taking out an external power line from an external power line storage box 9 to be connected with a power supply, if no external power supply is available, supplying power by adopting a reserve power supply 8, then scanning and registering a bar code on a chip body and a bacterial sample bar code through a bar code reading head 23 on an intelligent controller panel 6, and pressing a confirmation key on the intelligent controller panel 6 of the biochip reader to confirm; step two, after the code scanning registration of the chip is completed, opening the cabin door sealing ring 20 and the cabin door 2 of the biochip reader in sequence, and placing the chip on the chip reading platform 12 of the biochip reader to be fixed through a corresponding clamping seat; step three, confirming the position feedback information of the chip in the chip reading platform 12 through an intelligent controller panel; step four, after the position information of the placed chip in the chip reading platform 12 is confirmed to be correct, loading and placing the next chip according to the flow from the step one to the step two; step five, resetting the biochip reader hatch door 2 and the hatch door sealing ring 20 after all the chips to be loaded are placed in the chip reading platform 12; step six, a plurality of fine item parameters such as reading temperature, reading times and reading time are input through the intelligent controller panel 6, and the parameters are increased and decreased by pressing the upper key and the lower key in the operation page during setting. If the modified parameters are to be saved, the confirmation key in the operation page is pressed. If the chip temperature does not reach the reading temperature, the chip reading temperature compensation function key 35 of the intelligent controller panel 6 can start the heating function of the heating film to heat the chip so as to reach the reading temperature required by the chip. Before reading, firstly, the chip on the chip reading platform 12 is subjected to fluorescent scanning on the photo cavity, the intelligent controller 7 is used for intelligently matching the proper light source 10 and the corresponding light source system 11, and then the chip is read. The chip fluorescence detection dynamic data are wirelessly transmitted to the appointed object through the intelligent controller 7 of the chip reader during reading, and the corresponding data are synchronously stored in the database of the intelligent controller 7; step seven, after reading, turning off the power supply, opening the cabin door sealing ring 20, the cabin door 2 and the chip reading platform clamping seat in turn according to actual conditions, taking out the corresponding chips for recycling treatment, and resetting the chip reading platform clamping seat, the cabin door 2 and the cabin door sealing ring 20 in turn; step eight, the external power line is stored in the external power line storage box 9 for standby.
Example 3 application example of biochip reader for rapidly detecting sterilizing Effect
The biochip reader for rapidly detecting the sterilization effect in example 1 of the present invention performs sterilization effect detection on a single rapidly detecting sterilization effect chip with 20 bacterial chambers carrying 19 hydrogen peroxide plasma tube biological sterilization device sample bacterial chambers and 1 control bacterial chamber, which is cultured by a culture apparatus for detecting sterilization effect by an auxiliary chip (a culture apparatus for detecting sterilization effect by an auxiliary chip, chinese patent application No. 2023103704886), and compares the sterilization effect detection result with the existing 10-hole or 16-hole biochip reader on the market. As a result, the invention improves the efficiency of the detection process and reduces the results of false negative and false positive. The detection time of the sample bacterial pieces of the 380 hydrogen peroxide plasma tube biological sterilization device is saved by at least about 150 minutes compared with the detection time of the existing 10-hole or 16-hole biological readers on the market. In addition, the detection accuracy of the sterilization effect of the 380 cultured bacterial plates is 100%, which is 9.2% higher than that of the 10-hole or 16-hole culture holes of the existing biological reader.
Example 4 application example of biochip reader for rapidly detecting sterilizing Effect
The biochip reader for rapidly detecting the sterilizing effect in example 1 of the present invention performs sterilizing effect detection on a single rapidly detecting sterilizing effect chip with 20 bacterial chambers loaded with 19 pressure steam plasma tube biological sterilizing device sample bacterial chambers and 1 control bacterial chamber, which is cultured by a culture apparatus for detecting sterilizing effect by an auxiliary chip (a culture apparatus for detecting sterilizing effect by an auxiliary chip, chinese patent application No. 2023103704886), and compares the sterilizing effect detection result with the sterilizing effect detection result of the existing 10-hole or 16-hole biochip reader on the market. As a result, the invention improves the efficiency of the detection process and reduces the results of false negative and false positive. The incubation time for 285 pressure steam sterilized sample plaques is at least about 130 minutes less than the incubation time for 10-well or 16-well incubation wells of a commercial existing biological reader. In addition, the detection accuracy of the sterilizing effect of the 285 bacterial sheets is 100%, which is 10.7% higher than that of the 10-hole or 16-hole culture holes of the existing biological reader.
Example 5 application example of biochip reader for rapidly detecting sterilizing Effect
The biochip reader for rapidly detecting the sterilizing effect in example 1 of the present invention performs sterilizing effect detection on a single rapidly detecting sterilizing effect chip with 20 bacterial cells cavities carrying 19 ethylene oxide sterilizing sample bacterial cells and 1 control bacterial cell, which is cultured by a culture apparatus for detecting sterilizing effect by an auxiliary chip (a culture apparatus for detecting sterilizing effect by an auxiliary chip, chinese patent application No. 2023103704886), and compares the sterilizing effect detection result with the sterilizing effect detection result of the existing 10-hole or 16-hole biochip reader on the market. As a result, the invention improves the efficiency of the detection process and reduces the results of false negative and false positive. The incubation time for 190 ethylene oxide sterilized sample plaques is at least about 120 minutes less than the incubation time for 10-well or 16-well incubation wells of a commercial existing biological reader. In addition, the detection accuracy of the sterilization effect of the 190 bacterial sheets is 100%, which is 12.3% higher than that of the 10-hole or 16-hole culture holes of the existing biological reader.
Example 6 application example of biochip reader for rapidly detecting sterilizing Effect
The biochip reader for rapidly detecting the sterilization effect in the example 1 of the present invention performs sterilization effect detection on 30 rapidly detecting sterilization effect chips with 20 bacterial chambers carrying 19 hydrogen peroxide plasma tube biological sterilization device sample bacterial chambers and 1 control bacterial chamber, which are cultured by a culture apparatus for detecting sterilization effect by an auxiliary chip (a culture apparatus for detecting sterilization effect by an auxiliary chip, according to Chinese patent application number 2023103704886), and compares the sterilization effect detection results with the existing 10-hole or 16-hole biochip readers on the market. As a result, the invention improves the efficiency of the detection process and reduces the results of false negative and false positive. The detection time of the sample bacterial pieces of the 570 hydrogen peroxide plasma tube biological sterilization device is saved by at least about 740 minutes compared with the detection time of the existing 10-hole or 16-hole biological readers on the market. In addition, the detection accuracy of the sterilization effect of the cultured 570 bacterial sheets is 100%, which is 10.9% higher than that of the 10-hole or 16-hole culture holes of the existing biological reader.
Example 7 application example of biochip reader for rapidly detecting sterilizing Effect
The biochip reader for rapidly detecting the sterilization effect in the example 1 of the present invention performs sterilization effect detection on 30 rapidly detecting sterilization effect chips with 20 bacterial chambers carrying 19 hydrogen peroxide plasma tube biological sterilization device sample bacterial chambers and 1 control bacterial chamber, which are cultured by a culture apparatus for detecting sterilization effect by an auxiliary chip (a culture apparatus for detecting sterilization effect by an auxiliary chip, according to Chinese patent application number 2023103704886), and compares the sterilization effect detection results with the existing 10-hole or 16-hole biochip readers on the market. As a result, the invention improves the efficiency of the detection process and reduces the results of false negative and false positive. The detection time of the sample bacterial pieces of the 570 hydrogen peroxide plasma tube biological sterilization device is saved by at least about 925 minutes compared with the detection time of the existing 10-hole or 16-hole biological readers on the market. In addition, the detection accuracy of the sterilization effect of the cultured 570 bacterial sheets is 100%, which is 14.1% higher than that of the 10-hole or 16-hole culture holes of the existing biological reader.
Example 8 application example of biochip reader for rapidly detecting sterilizing Effect
The biochip reader for rapidly detecting the sterilization effect in the example 1 of the present invention performs sterilization effect detection on 30 rapidly detecting sterilization effect chips with 20 bacterial chambers carrying 19 hydrogen peroxide plasma tube biological sterilization device sample bacterial chambers and 1 control bacterial chamber, which are cultured by a culture apparatus for detecting sterilization effect by an auxiliary chip (a culture apparatus for detecting sterilization effect by an auxiliary chip, according to Chinese patent application number 2023103704886), and compares the sterilization effect detection results with the existing 10-hole or 16-hole biochip readers on the market. As a result, the invention improves the efficiency of the detection process and reduces the results of false negative and false positive. The incubation time for 570 ethylene oxide sterilized sample plaques is at least about 1110 minutes less than the incubation time for 10-well or 16-well incubation wells of a commercial existing biological reader. In addition, the detection accuracy of the sterilization effect of the cultured 570 bacterial sheets is 100%, which is 16.8% higher than that of the 10-hole or 16-hole culture holes of the existing biological reader.
From the above embodiment, the invention well solves the aging problem for the sterilization effect detection of a plurality of bacteria tablet cavity chips, realizes the rapid detection of the sterilization effect of 'after sterilization and results', greatly promotes the efficient development of the chip laboratory sterilization effect evaluation work, and greatly reduces the working time of inspectors. For clinical treatment, the time for waiting for medical instruments before operation of a patient can be greatly shortened, the accuracy of the starting time of the medical instrument operation is improved, the duration of the medical instrument operation is shortened, the turnover rate between the medical instrument operations is increased, the turnover times of a sickbed of the medical instrument are accelerated, and the turnover rate of the sickbed of the medical instrument is improved. Meanwhile, the sterilization effect of the medical instrument can be determined in a large scale in the shortest time, the inspection period of the sterilization effect of the medical instrument is shortened, unqualified sterilization medical instruments can be recalled in the shortest time, the final sterilization effect is obtained before the medical instrument is used, adverse effects caused by sterilization failure of the medical instrument can be reduced, and further the occurrence of infection is reduced and controlled.
The foregoing examples are set forth in order to provide a more thorough description of the present invention, and are not intended to limit the scope of the invention, since modifications of the present invention, in which equivalents thereof will occur to persons skilled in the art upon reading the present invention, are intended to fall within the scope of the invention as defined by the appended claims.
Claims (8)
1. A biochip reader for rapidly detecting sterilization effect, which is characterized in that: the reader comprises a chip reading cabin body and a cabin door hinged on the chip reading cabin body; the chip reading cabin body consists of a first baffle, a second baffle and a third baffle which are parallel from top to bottom; a panel of the intelligent controller is arranged on the first partition board, and the intelligent controller is arranged below the first partition board; a reserve power supply and an external power supply line storage box are arranged on the second partition board, a light source and a light source system switching controller are arranged below the second partition board, and the light source system switching controller are electrically connected with the reserve power supply and the external power supply; the third partition board is provided with a chip reading platform, an optical transceiver, a fluorescence detector and a cabin door switch state sensor; the chip reading platform is provided with a chip placement sensor, a chip temperature sensor, a chip placement abnormality or culture abnormality alarm device and a heating film; the chip reading platform, the optical transceiver and the fluorescence detector cabin door switch state sensor are all electrically connected with the intelligent controller through connecting wires, and the intelligent controller is electrically connected with an external power supply and a reserve power supply.
2. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the intelligent controller performs intelligent control through an intelligent control system, the intelligent control system comprises a micro server, a sensing information processing module arranged in a chip of a chip reading platform, a sensing information processing module of the chip temperature of the chip reading platform, an alarm information processing module of the chip reading platform, a sensing information processing module of a cabin door switch state, an information processing module of a light source, an information processing module of an optical transceiver and an information processing module of a fluorescence detector, and the micro server comprises a processor and a database, wherein the sensing information processing module, the alarm information processing module and the information processing module are electrically connected with the processor.
3. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the panel of the intelligent controller comprises a bar code reading head, a liquid crystal display screen, a reader function key, a chip fluorescence detection dynamic data monitoring wireless transmission key, an equipment log key, a fault self-diagnosis key, an equipment remote control key and an equipment start/stop key; the reader function keys of the intelligent controller comprise a chip placement confirmation key, a chip bar code scanning key, a bacterial chip sample bar code scanning key, a cabin door switch state display key, a chip reading temperature compensation function key and a light source intelligent selection key; the chip fluorescence detection dynamic data monitoring wireless transmission key of the intelligent controller comprises a chip fungus cavity fluorescence intensity-time curve recording function key and a chip fungus cavity fluorescence detection image-time recording key.
4. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the number of the chips which can be placed on the chip reading platform is 1-50.
5. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the second separator is made of a mixed material of graphene and zirconium boride, and the mass content of the zirconium boride in the mixed material is 2% -12%.
6. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the third separator is made of a mixed material of carbon fibers and silicon boride, and the mass content of the silicon boride in the mixed material is 23% -38%.
7. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the chip reading platform is made of porous carbon aerogel materials, and the pore diameter of the porous carbon aerogel is between 0.1 and 0.5 micron.
8. The biochip reader of claim 1, wherein the biochip reader is configured to rapidly detect sterilization effects: the application method of the biochip reader for rapidly detecting the sterilization effect comprises the following steps: firstly, switching on a power supply, registering a bar code on a chip body and a bar code of a bacterial sample by a bar code reading head on an intelligent controller panel, and pressing a confirmation key on the intelligent controller panel to confirm; after the code scanning registration of the chip is completed, opening a cabin door sealing ring and a cabin door of the chip reader in sequence, and placing the chip to a reading platform of the chip reader to be fixed through a corresponding clamping seat; step three, confirming the position feedback information of the chip in the chip reader through the intelligent controller panel; step four, after the position information of the placed chip in the chip reader is confirmed to be correct, loading and placing the next chip according to the flow from the step one to the step three; resetting a chip reader cabin door and a cabin door sealing ring after all chips to be loaded are placed in the reader; step six, a plurality of fine item parameters such as reading temperature, reading times, reading time and the like are input through an intelligent controller panel, and the parameters can be increased or decreased by pressing upper and lower keys in an operation page during setting. If the modified parameters are to be saved, the confirmation key in the operation page is pressed, the heating film can be started to heat so as to reach the reading temperature required by the chip. Before reading, the chip is first matched with proper light source through fluorescent scanning to the chip and then read. The chip fluorescence detection dynamic data are transmitted to the appointed object in a wireless way through the intelligent controller of the chip reader during reading, and the corresponding data are synchronously stored in the database of the intelligent controller; step seven, after reading, turning off the power supply, opening the cabin door sealing ring, the cabin door and the chip reading platform clamping seat in turn according to actual conditions, taking out the corresponding chips for recycling treatment, and resetting the chip reading platform clamping seat, the cabin door and the cabin door sealing ring in turn; and step eight, the external power line is accommodated in the external power line accommodating box for standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310401476.5A CN116396854A (en) | 2023-04-15 | 2023-04-15 | Biochip reader capable of rapidly detecting sterilization effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310401476.5A CN116396854A (en) | 2023-04-15 | 2023-04-15 | Biochip reader capable of rapidly detecting sterilization effect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116396854A true CN116396854A (en) | 2023-07-07 |
Family
ID=87017722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310401476.5A Pending CN116396854A (en) | 2023-04-15 | 2023-04-15 | Biochip reader capable of rapidly detecting sterilization effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116396854A (en) |
-
2023
- 2023-04-15 CN CN202310401476.5A patent/CN116396854A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9550970B2 (en) | Culture systems, apparatus, and related methods and articles | |
| JP7046845B2 (en) | Modular assay reader device | |
| CN106701566B (en) | A kind of collection nucleic acid extraction expands, is detected on integrated mechanical device | |
| US20210285977A1 (en) | Automated Medical Diagnostic System and Method | |
| US7395172B2 (en) | Analyzer and computer program product | |
| JP2006507837A (en) | Biological growth plate scanner with automatic image processing profile selection | |
| JP5606285B2 (en) | Analysis method and apparatus | |
| CN220503074U (en) | Biochip reader capable of rapidly detecting sterilization effect | |
| CN112300927A (en) | Portable full-automatic micro-fluidic nucleic acid detects all-in-one | |
| JP2020507071A (en) | Portable digital diagnostic device | |
| CN116396854A (en) | Biochip reader capable of rapidly detecting sterilization effect | |
| CN116322999A (en) | Point-of-care diagnostic instrument workflow | |
| CN219972312U (en) | Supplementary chip detects cultivation appearance of sterilization effect | |
| CN103592267A (en) | Optical correction device, biological detection correction system and operation method thereof | |
| WO2018223539A1 (en) | Clinical test information processing system and method | |
| CN116355749A (en) | Supplementary chip detects cultivation appearance of sterilization effect | |
| CN202794198U (en) | Biochemical analysis and diagnosis platform | |
| CN106591106B (en) | A kind of rotating disc type laser scanning reading device | |
| CN111879769A (en) | Device for reading medical detection kit | |
| CN111458493B (en) | Multifunctional biochemical analyzer | |
| CN209432842U (en) | A kind of test response prompting system | |
| CN102183670B (en) | Multiparameter in-vitro immediate automatic detection analytical device and detection method | |
| CN212060292U (en) | Porous microplate application of sample display device | |
| CN210572325U (en) | POCT analysis device | |
| CN221028439U (en) | Portable nucleic acid detecting device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |