CN106591106B - A kind of rotating disc type laser scanning reading device - Google Patents
A kind of rotating disc type laser scanning reading device Download PDFInfo
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- CN106591106B CN106591106B CN201611198072.7A CN201611198072A CN106591106B CN 106591106 B CN106591106 B CN 106591106B CN 201611198072 A CN201611198072 A CN 201611198072A CN 106591106 B CN106591106 B CN 106591106B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
The present invention relates to a kind of rotating disc type laser scanning reading devices.It include: cartridge；Cartridge heater: motor driving, heater the inside are embedded with magnet；Motor control mechanism: the pressing plate shaft right part of screw rod right part, stretching box body that box body is stretched out in cartridge is connected with motor, by motor control cartridge sampling probe transverse direction and moves up and down；Integrated circuit board；Barcode reader；Scanner: including high speed rotation platform, laser emitter and photomultiplier tube, high-speed motion, laser emitter, photomultiplier tube are oppositely arranged the chip of cartridge bottom on the rotating pan.All reagents needed for nucleic acid extraction of the present invention, amplification and detection are previously placed in cartridge, avoid the chance of pollution；The PCR product of high concentration is closed in inside cartridge, and pollution sources have also obtained effective control.Set molecule diagnoses nucleic acid extraction, amplification, detection and scanning in one, realizes automation, closure.
Description
(1) technical field
The invention belongs to biomedical devices technical field, in particular to a kind of rotating disc type laser scanning reading device.
(2) background technique
There are three key steps altogether for molecular diagnosis: nucleic acid extraction, amplification and detection.
The necessity of amplification: the purpose of amplification is to increase signal-to-noise ratio.All diagnosis are all to seek optimal noise
Than infectious diseases is especially true.Such as when the infection of blood source, may there was only 20 bacteriums inside one milliliter of blood of patient, and it is same
As soon as then having million leucocytes inside sample milliliter blood, the nucleic acid and other macromoleculars inside these leucocytes become detection
Noise increases signal-to-noise ratio so the purpose of amplification is exactly to expand signal.It does not expand, is difficult to required for obtaining diagnosis special
Property and sensibility.
Automation is needed for market: the management of clinical labororatory and operator are not the doctors in universities and colleges and R&D institution
Master, their typically no solid Protocols in Molecular Biologies are basic, if therefore thinking to promote a molecular diagnosis skill on a large scale
Art, the degree of automation of the technology have to height, are just avoided that mistake in this way, improve diagnosis efficiency.Real-time PCR is actually
Have been completed automatic amplification and detection, still, multiplex amplification is not well solved with the problem of detection.
Closed system is required by market: it is so-called not close, open pipe lid is also exactly needed after PCR amplification, highly concentrated
The PCR product of degree, which is taken out, carries out next step reaction.This open program easily causes laboratory amplification product pollution,
Lead to the result of false positive.All people for being PCR both know about, and pollution will occur sooner or later, and once occurring very
Hardly possible removal, impassable difficulty is brought to diagnostic test.
In order to avoid pollution, the even hospital in very base, it is also desirable to molecular diagnostic laboratories be configured with multiple
Separate chamber: sample extracts, and reaction is established, and amplification and detection etc. will separate, and avoids pollution.
The each step of molecular diagnosis has many methods, and each method has different reagents that can be selected, each
Method has some producers to produce different self-reacting devices again, therefore, is how to the challenge of clinical use person
These reagents and instrument are selected and integrate, so that each step has the result of optimization.
The solution of one optimal molecular diagnosis should be: extract, amplification, detection, the Trinity；Entirely certainly
It is dynamic, totally-enclosed, anti-pollution, triathlon.
On November 19th, 2014 discloses a kind of automatic detection reagent cartridge, the automatic detection reagent cartridge include screw rod,
Screw rod knob, connector, sampling probe, negative pressure generator, compression bar needle, pressing plate, pressing plate knob, injection port, sample cell, agent bin,
Hybridize slot, nucleic acid extraction, amplification and detection process are all completed in tray interior, and whole process closing does not contact with the external world, solves reality
The technical issues of testing room and clinical PCR pollution.Although disclosing the automatic detection reagent cartridge and external mechanical part cooperation makes
With external machinery is controlled by control system, and instruction can be inputted in control system, sets according to detecting step and takes liquid interval
And reagent etc. in which agent bin is taken, but how the cartridge is used cooperatively with external mechanical part, how to pass through control
The step of external mechanical basis of system control is set takes reagent, how to carry out nucleic acid extraction, amplification and subsequent PCR amplification and produces
How object is reacted with the chip at cartridge bottom, how to be tested and analyzed, how data result reads etc. all without reference to for this
For the technical staff of field, these be all it is unclear, i.e., automatic detection reagent cartridge can not accomplish really to promote and apply.
The solution of one optimal molecular diagnosis should be just: extract, amplification, detection, the Trinity；Entirely certainly
It is dynamic, totally-enclosed, anti-pollution, triathlon.The practicability and maturity for measuring a technology platform also will see whether it completes
This Trinity, the perfect integration of triathlon, and present invention provides so a kind of equipment.The equipment also by with it is primary
Property cartridge match to avoid pollution: because PCR reacts and in producer has just set up (reagent is previously placed in cartridge),
Also the chance of pollution is avoided；In addition, the PCR product of high concentration is closed in inside cartridge, pollution sources have also been obtained effectively
Control.It solves the problems, such as that pollution not only allows user to trust, also solves shortage to basic hospital, capital cost is excessively high
Problem.
(3) summary of the invention
In order to compensate for the shortcomings of the prior art, the present invention provides a kind of rotating disc type laser scanning reading devices, extract, expand
Increase, detection, the Trinity；Automatically, totally-enclosed, anti-pollution, triathlon.
The present invention is achieved through the following technical solutions:
A kind of rotating disc type laser scanning reading device, is characterized in that and includes:
Cartridge: including box body, being arranged injection port on the top surface of box body, sealing cover, box body bottom difference are arranged on injection port
Sample cell, agent bin, hybridization slot are set, and chip is arranged in hybridization trench bottom, and having on the chip can be with PCR product specific hybrid
Detection probe, wherein sample cell is oppositely arranged with injection port, and screw rod, sampling probe are respectively set in box body, and the screw rod is left and right
Both ends are rotatablely connected through bearing three, bearing four and box body corresponding side respectively, and screw rod is horizontally disposed, and the right end of screw rod stretches out box body,
Connector is set on screw rod, which is connected with screw rod by screw thread, sampling probe accommodating hole is provided on the top surface of connector,
The central axis of sampling probe accommodating hole and the central axis of screw rod, the first spring of sampling probe accommodating hole bottom fixed setting, first
Spring and sampling probe accommodating hole axis are arranged, and the first spring is upwardly extended by sampling probe accommodating hole bottom and stretches out sampling probe accommodating
Hole, sampling probe are set in the first spring, and sampling probe is connected to by pipeline with negative pressure generator, negative pressure generator include shell,
Piston and piston rod, upper cover of piston rod have second spring, and the free end of piston rod is fixedly connected with the left end of compression bar needle, compression bar needle
Right end stretches out box body, is used for propelling piston bar, is provided with pressing plate above sampling probe, pressing plate include pressing plate ontology and with pressing plate sheet
The pressing plate shaft of body connection, wherein pressing plate shaft is arranged in parallel with screw rod, and the left end of pressing plate shaft is connected by bearing one and box body rotation
It connects, the right end of pressing plate shaft is rotatablely connected by bearing two and box body；
Cartridge heater: motor driving, heater the inside are embedded with magnet；
Motor control mechanism: the screw rod right part of box body is stretched out in cartridge, stretches out the pressing plate shaft right part of box body and horse
Up to be connected: by motor control cartridge sampling probe transverse direction and move up and down；
Barcode reader: cartridge is inserted into instrument, carries out identifying processing to cartridge by barcode reader, analysis result is straight
It connects and is aggregated into computer.
The rotating disc type laser scanning reading device, rotating disc type laser scanning reading device can handle 1-4 card simultaneously
Box, each cartridge processing unit is independent work；
Scanner: including high speed rotation platform, laser emitter and photomultiplier tube, the chip of cartridge bottom is in rotating disk
Upper high-speed motion, several cartridge positions are arranged above high speed rotation platform, and laser emitter and photomultiplier tube are located at high speed and revolve
Turn the lower section of platform, laser emitter, photomultiplier tube are oppositely arranged.
The rotating disc type laser scanning reading device, there are also waste liquid tanks for box body bottom.Nucleic acid extraction, amplification, detection institute
All reagents needed are respectively placed in reagent cabin, and the open end in reagent cabin is closed by masking foil.
Sample cell, reagent cabin, waste liquid tank, the top setting porous plate for hybridizing slot, the left and right end of porous plate is respectively and in box body
Wall fixed setting, hole on porous plate and sample cell, reagent cabin, waste liquid tank, the open end for hybridizing slot respectively correspond.
Inboard wall of cartridge is equipped with the pressing plate limit plate for limiting pressing plate shaft rotation angle, and pressing plate limit plate is located under pressing plate ontology
Side, the central axis of pressing plate limit plate are vertical with pressing plate shaft.
Bearing three, four installation place of bearing are respectively mounted gasket.
The invention has the advantages that: all reagents needed for nucleic acid extraction of the present invention, amplification, detection to be previously placed in cartridge,
Avoid the chance of pollution；In addition, the PCR product of high concentration is closed in inside cartridge, pollution sources have also obtained effective control.
It solves the problems, such as that pollution not only allows user to trust, also solves shortage, the excessively high problem of capital cost to basic hospital.
Set molecule diagnoses nucleic acid extraction, amplification and detection and scanning in one, realizes automation, closure, device is easy to use.
(4) Detailed description of the invention
The present invention will be further described below with reference to the drawings.
Attached drawing 1 is the structural schematic diagram of cartridge of the present invention；
Attached drawing 2 is the positional diagram of pressing plate of the present invention and pressing plate limit plate；
Attached drawing 3 is the positional diagram of screw rod of the present invention and connector；
Attached drawing 4 is the structural schematic diagram of rotating disc type laser scanning reading device of the present invention；
Attached drawing 5 is the structural schematic diagram of scanner of the present invention,
In figure, 1 box body, 11 injection ports, 2 pressing plate shafts, 21 bearings one, 23 bearings two, 24 pressing plate limit plates, 25 pressing plate sheets
Body, 26 pressing plate shafts, 3 sampling probes, 31 pistons, 32 negative pressure generators, 33 second springs, 34 compression bar needles, 35 first springs, 36 pipes
Road, 4 screw rods, 41 bearings three, 42 bearings four, 44 connectors, 5 sample cells, 61 agent bins one, 62 agent bins two, 63 agent bins three
, 64 agent bins four, 65 agent bins five, 66 agent bins six, 67 agent bins seven, 68 waste liquid tanks, 7 hybridization slots, 71 glass-chips, 8
Tinfoil paper, 9 porous plates, 10 cartridge heaters, 12 integrated circuit boards, 13 barcode readers, 14 high speed rotation platforms, 15 laser hair
Emitter, 16 photomultiplier tubes, 17 cartridge positions, 18 motor control mechanism.
(5) specific embodiment
The present embodiment rotating disc type laser scanning reading device includes cartridge, high speed rotation platform 14, laser emitter 15, light
Electric multiplier tube 16, cartridge position 17.
Cartridge includes box body 1, and injection port 11 is arranged on the top surface of box body 1, sealing cover, 1 bottom of box body is arranged on injection port 11
Sample cell 5, agent bin 61-67, hybridization slot 7, waste liquid tank 68 is respectively set in portion, and agent bin 61-67 is seven, respectively agent bin
One 61, agent bin 2 62, agent bin 3 63, agent bin 4 64, agent bin 5 65, agent bin 6 66, agent bin seven
67 ；Reagent needed for being placed with nucleic acid extraction, PCR amplification and hybridization check in seven agent bin 61-67 respectively, and seven reagents
The oral area of storehouse 61-67 is closed by tinfoil paper 8.Hybridize 7 bottom of slot and glass-chip 71 is set, there is energy and PCR on the glass-chip 71
The detection probe of product specificities hybridization, PCR product have fluorescent marker (being placed on reverse primer), so hybridization is completed
The identification of specific target spot.Agent bin 1, agent bin 2 62, agent bin 3 63, agent bin 4 64, agent bin five
65, agent bin 6 66, agent bin 7 67, waste liquid tank 68, sample cell 5, hybridization 7 top of slot are provided with porous plate 9, porous
Plate 9 is fixedly connected with 1 inner wall of box body, the hole on porous plate 9 respectively correspond agent bin 1, agent bin 2 62,
Agent bin 3 63, agent bin 4 64, agent bin 5 65, agent bin 6 66, agent bin 7 67, waste liquid tank 68, sample cell
5, hybridize the open end of slot 7.Wherein, sample cell 5 is oppositely arranged with injection port 11.Screw rod 4, sampling probe are respectively set in box body 1
3, the left and right both ends of the screw rod 4 are rotatablely connected through bearing 3 41, bearing 4 42 and 1 corresponding side of box body respectively, and 4 level of screw rod is set
It sets, the right end of screw rod 4 stretches out box body 1, and connector 44 is arranged on screw rod 4, which is connected with screw rod 4 by screw thread, is turned
Screw rod 4, connector 44 and 4 relative motion of screw rod are moved, is provided with sampling probe accommodating hole 45, sampling probe on the top surface of connector 44
The first spring is fixedly installed in the central axis of accommodating hole 45 and the central axis of screw rod 4,45 bottom of sampling probe accommodating hole
35, the first spring 35 is arranged with 45 axis of sampling probe accommodating hole, the first spring 35 from 45 bottom of sampling probe accommodating hole to
It is upper extend simultaneously stretch out sampling probe accommodating hole 45, sampling probe 3 is set in the first spring 35, rotary screw 4, sampling probe 3 with
Connector 44 agent bin, sample cell 5, waste liquid tank 68, hybridization slot 7 between move, sampling probe 3 pass through pipeline 36
It is connected to negative pressure generator 32, negative pressure generator 32 includes that shell, piston 31 and piston rod, upper cover of piston rod have second
Spring 33, the free end of piston rod are fixedly connected with the left end of compression bar needle 34, and the right end of compression bar needle 34 stretches out box body 1, uses
In propelling piston bar, when not pushing compression bar needle 34, second spring 33 pops into position compression bar needle 34, sampling probe 3
Top is provided with pressing plate 2, and pressing plate 2 includes pressing plate ontology 25 and the pressing plate shaft 26 connecting with pressing plate ontology 25, wherein
Pressing plate shaft 26 is arranged in parallel with screw rod 4, and the left end of pressing plate shaft 26 is rotatablely connected by bearing 1 and box body 1,
The right end of pressing plate shaft 26 is rotatablely connected by bearing 2 23 and box body 1, and the right end of pressing plate shaft 26 stretches out box body 1.Pressure
Plate ontology 25 for pressing down on sampling probe 3, rotary pressure plate axis 26, press downwards with dynamic pressure plate ontology 25 by pressing plate shaft 26
Sampling probe 3 is pressed, not when rotary pressure plate axis 26, the first spring 35 pops into position pressing plate ontology 25.1 inner wall of box body is also
It is fixedly installed the pressing plate limit plate 24 that angle is rotated for limiting pressing plate shaft 26, pressing plate limit plate 24 is located at pressing plate ontology
25 lower sections, the central axis of pressing plate limit plate 24 is vertical with pressing plate shaft 26, when pressing plate shaft 26 goes to certain angle, pressing plate
25 contact with pressing plate limit plate 24, and pressing plate shaft 26 cannot continue to rotate, for the ease of taking the reagent in agent bin 61-67,
Pressing plate limit plate 24 limits the rotation angle of pressing plate shaft 26 less than 150 °.
Above-mentioned cartridge is actually progress inside a laboratory technician " clone " to this disposable plastic casing
The operation of Routine Test Lab liquid.The reagent of all reactions is all just put into the bottom of cartridge in manufacturer in advance, does nucleic acid extraction
Magnetic bead liquid be stored in cartridge bottom in advance.After amplified reaction, automatic liquid relief robs a reaction product and is all put into hybridization slot 7
There are printed glass-chip 71 in the inside, hybridization 7 bottom of slot, there is the detection probe of energy and PCR product specific hybrid above, because
Fluorescent marker (being placed on reversed general primer) is had for PCR product, so the Multiple recognition of specific target spot is completed in hybridization.
Hybridization solution, dcq buffer liquid etc. all have previously been stored in cartridge bottom, used liquid, the liquid containing high concentration PCR product
It is all stored in the waste liquid tank of cartridge bottom.Once injection port 11 is added in sample, the mouth is then closed, entire sample extracts,
Amplification and detection process are all completed inside this cartridge, whole process closing, and extraneous contact of getting along well will not generate pollution.
The heater of 10: three independent controls of cartridge heater does PCR reaction.Magnet is also embedded inside heater, institute
It can also be solved inside cartridge by the nucleic acid extraction operation based on magnetic bead.Heater also provides constant temperature for hybridization, controls
The specificity of hybridization reaction.
Motor control mechanism 18: 4 right part of screw rod of box body 1 is stretched out in cartridge, stretches out 26 right part of pressing plate shaft of box body 1
It is connected with motor: by 3 transverse direction of motor control cartridge sampling probe and moves up and down.
Power supply control and external information interface.
Integrated circuit board 12: all automation movements of control.
Barcode reader 13: cartridge is inserted into instrument, carries out identifying processing, analysis knot to cartridge by barcode reader 13
Fruit is directly aggregated into computer, directly can issue doctor in a manner of e-mail or short message.
Every instrument can handle simultaneously four samples (upper machine at random, do not have to equal full four together on).At each cartridge
Managing unit is all independent work, and different units can run the cartridge of different content, sample and different diseases to different patients
Carry out molecular diagnosis.If clinic needs while handling more samples, 12 processors that can connect are simultaneously to 48
A sample is handled.
Extraction, amplification and miscellaneous are had been completed by collection nucleic acid extraction, the cartridge that expands, be detected on integrated mechanical device
It hands over and programs, the final taches such as cleaning is exactly to read detection data.Laboratory technician only need cartridge from collection nucleic acid extraction, amplification,
It is detected on taking-up (cartridge after treatment instrument and software have prompt) in integrated mechanical device and is put into laser scanning reading dress
The inside is set, scanning in 1-5 minutes can carry out full-automatic reading data to 1-4 cartridge.The scanner includes high speed rotation
Platform 14, laser emitter 15 and photomultiplier tube 16, high-speed motion, high speed rotation are flat on the rotating pan for the chip of cartridge bottom
4 cartridge positions 17 are arranged in platform 14 above, and 17 cross section of cartridge position is quadrant.Laser emitter 15 and photomultiplier transit
Pipe 16 is located at the lower section of high speed rotation platform 14, and laser emitter 15, photomultiplier tube 16 are oppositely arranged.The chip of cartridge bottom
High-speed motion on the rotating pan, laser as long as simple scanning once as long as can be scanned reading to four cartridges.
Claims (7)
1. a kind of rotating disc type laser scanning reading device, it is characterised in that: include:
Cartridge: including box body, injection port is set on the top surface of box body, sealing cover is set on injection port, box body bottom is respectively set
Chip is arranged in sample cell, agent bin, hybridization slot, hybridization trench bottom, and having on the chip can be with the detection of PCR product specific hybrid
Probe, wherein sample cell is oppositely arranged with injection port, and screw rod, sampling probe, the left and right both ends of screw rod are respectively set in box body
It is rotatablely connected respectively through bearing three, bearing four and box body corresponding side, screw rod is horizontally disposed, and the right end of screw rod stretches out box body, screw rod
Upper setting connector, the connector are connected with screw rod by screw thread, and sampling probe accommodating hole is provided on the top surface of connector, sampling
The first spring, the first spring is fixedly installed in the central axis of needle accommodating hole and the central axis of screw rod, sampling probe accommodating hole bottom
It is arranged with sampling probe accommodating hole axis, the first spring is upwardly extended by sampling probe accommodating hole bottom and is stretched out sampling probe accommodating hole,
Sampling probe is set in the first spring, and sampling probe is connected to by pipeline with negative pressure generator, and negative pressure generator includes shell, piston
And piston rod, upper cover of piston rod have second spring, the free end of piston rod is fixedly connected with the left end of compression bar needle, the right end of compression bar needle
Box body is stretched out, propelling piston bar is used for, pressing plate is provided with above sampling probe, pressing plate includes pressing plate ontology and connects with pressing plate ontology
The pressing plate shaft connect, wherein pressing plate shaft is arranged in parallel with screw rod, and the left end of pressing plate shaft is rotatablely connected by bearing one and box body, pressure
The right end of board shaft is rotatablely connected by bearing two and box body；
Cartridge heater: motor driving, heater the inside are embedded with magnet；
Motor control mechanism: stretch out in cartridge the screw rod right part of box body, stretch out the pressing plate shaft right part of box body with motor phase
Even, it by motor control cartridge sampling probe transverse direction and moves up and down；
Barcode reader: cartridge is inserted into instrument, carries out identifying processing to cartridge by barcode reader, analysis result is directly converged
Always arrive computer；
Scanner: including high speed rotation platform, laser emitter and photomultiplier tube, the chip of cartridge bottom is high on the rotating pan
Speed moves, and it is flat positioned at high speed rotation that several cartridge positions, laser emitter and photomultiplier tube are arranged above high speed rotation platform
The lower section of platform, laser emitter, photomultiplier tube are oppositely arranged.
2. rotating disc type laser scanning reading device according to claim 1, it is characterised in that: rotating disc type laser scanning is read
Device handles 1-4 cartridge simultaneously, and each cartridge processing unit is independent work.
3. rotating disc type laser scanning reading device according to claim 2, it is characterised in that: there are also waste liquids for box body bottom
Slot.
4. rotating disc type laser scanning reading device according to claim 3, it is characterised in that: the open end in reagent cabin is by tin
Foil paper closing.
5. rotating disc type laser scanning reading device according to claim 1 to 4, it is characterised in that: sample cell, reagent
Cabin, waste liquid tank, the top setting porous plate for hybridizing slot, the left and right end of porous plate is fixedly installed with inboard wall of cartridge respectively, on porous plate
Hole and sample cell, reagent cabin, waste liquid tank, the open end for hybridizing slot respectively correspond.
6. rotating disc type laser scanning reading device according to claim 5, it is characterised in that: inboard wall of cartridge, which is equipped with, limits pressure
Board shaft rotates the pressing plate limit plate of angle, and pressing plate limit plate is located at below pressing plate ontology, the central axis and pressing plate of pressing plate limit plate
Axis is vertical.
7. rotating disc type laser scanning reading device according to claim 6, it is characterised in that: bearing three, bearing four are installed
Place is respectively mounted gasket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611198072.7A CN106591106B (en) | 2016-12-22 | 2016-12-22 | A kind of rotating disc type laser scanning reading device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611198072.7A CN106591106B (en) | 2016-12-22 | 2016-12-22 | A kind of rotating disc type laser scanning reading device |
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| CN106591106A CN106591106A (en) | 2017-04-26 |
| CN106591106B true CN106591106B (en) | 2019-08-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611198072.7A Active CN106591106B (en) | 2016-12-22 | 2016-12-22 | A kind of rotating disc type laser scanning reading device |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115216397A (en) * | 2022-09-01 | 2022-10-21 | 深圳零一生命科技有限责任公司 | Full-automatic nucleic acid extraction amplification diagnosis all-in-one |
| CN219266126U (en) * | 2022-12-14 | 2023-06-27 | 广东九纵智能科技有限公司 | Visual inspection universal platform |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102445557A (en) * | 2011-09-27 | 2012-05-09 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immunity analyzer and detection method thereof |
| CN104152349A (en) * | 2014-07-29 | 2014-11-19 | 山东艾克韦生物技术有限公司 | Automatic detection reagent cassette |
| CN105873681A (en) * | 2013-11-18 | 2016-08-17 | 尹特根埃克斯有限公司 | Cartridges and instruments for sample analysis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8287820B2 (en) * | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102445557A (en) * | 2011-09-27 | 2012-05-09 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immunity analyzer and detection method thereof |
| CN105873681A (en) * | 2013-11-18 | 2016-08-17 | 尹特根埃克斯有限公司 | Cartridges and instruments for sample analysis |
| CN104152349A (en) * | 2014-07-29 | 2014-11-19 | 山东艾克韦生物技术有限公司 | Automatic detection reagent cassette |
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| CN106591106A (en) | 2017-04-26 |
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