CN116369309B - Stem cell low-temperature preservation device - Google Patents
Stem cell low-temperature preservation device Download PDFInfo
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- CN116369309B CN116369309B CN202310629636.1A CN202310629636A CN116369309B CN 116369309 B CN116369309 B CN 116369309B CN 202310629636 A CN202310629636 A CN 202310629636A CN 116369309 B CN116369309 B CN 116369309B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 45
- 238000004321 preservation Methods 0.000 title abstract description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 336
- 239000007788 liquid Substances 0.000 claims abstract description 196
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 168
- 238000007789 sealing Methods 0.000 claims description 22
- 230000007246 mechanism Effects 0.000 claims description 14
- 238000005138 cryopreservation Methods 0.000 claims description 8
- 230000008676 import Effects 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 230000003028 elevating effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Life Sciences & Earth Sciences (AREA)
- Thermal Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a low-temperature storage device for stem cells, which relates to the field of local preservation of human tissues and comprises a liquid nitrogen tank shell, a liquid nitrogen tank liner and a lofting frame arranged in the liquid nitrogen tank liner, wherein drawers for independently storing a plurality of groups of stem cells are arranged at the lofting frame, an inlet is formed in the top end of the liquid nitrogen tank shell, and a small-caliber inlet is formed at the liquid nitrogen tank.
Description
Technical Field
The invention relates to the field of local preservation of human tissues, in particular to a stem cell low-temperature preservation device.
Background
In order to maintain the activity of stem cells, the stem cells need to be stored in a low-temperature environment when the stem cells are stored, the existing stem cells are mainly stored in a liquid nitrogen tank, liquid nitrogen is added into the liquid nitrogen tank, then the stem cells are placed in a lofting frame, and the lofting frame is placed in the liquid nitrogen tank with the low-temperature inside.
When the staff places the lofting frame that has deposited stem cells inside the liquid nitrogen container or will deposit the lofting frame of stem cells and follow the inside taking out of liquid nitrogen container, the liquid nitrogen of liquid nitrogen container inside can flow in the outside environment through the jar mouth at liquid nitrogen container top, causes a large amount of losses of liquid nitrogen to reveal, and the staff is when taking the lofting frame, and the liquid nitrogen of low temperature can cause the discomfort for the staff.
The main reason for the problem is that when a worker places a lofting frame for storing stem cells, a sealing cover and the like at the top tank opening of the liquid nitrogen tank are required to be opened, the caliber of the tank opening of the liquid nitrogen tank is large, and when the liquid nitrogen tank is in a naked state, liquid nitrogen in the liquid nitrogen tank can rapidly flow out of an external environment through the tank opening, so that loss of the liquid nitrogen is caused;
in addition, because the liquid nitrogen temperature inside the liquid nitrogen tank is lower, and the temperature of external environment is greater than the temperature inside the liquid nitrogen tank for convection current takes place for the inside air with the external world of liquid nitrogen tank, the inside of liquid nitrogen tank can exist the air in the more external environment, leads to the inside environmental stability of liquid nitrogen tank to be destroyed, very easily leads to stem cell to preserve inefficacy, to above-mentioned condition, we propose a stem cell low temperature preservation device.
Disclosure of Invention
Aiming at the defects of the prior art, the invention discloses a low-temperature stem cell preservation device, which aims to solve the problems that when a lofting frame for storing stem cells is placed in a liquid nitrogen tank or taken out from the inside of the liquid nitrogen tank in the background art, liquid nitrogen in the liquid nitrogen tank flows into an external environment through a tank opening at the top of the liquid nitrogen tank, so that a large amount of liquid nitrogen is lost and leaked, and when a worker takes the lofting frame, the low-temperature liquid nitrogen can cause discomfort to the worker.
In order to achieve the above purpose, the invention is realized by the following technical scheme: a stem cell cryopreservation apparatus comprising:
the device comprises a liquid nitrogen tank shell, a liquid nitrogen tank liner and a lofting frame arranged in the liquid nitrogen tank liner, wherein drawers for independently storing a plurality of groups of stem cells are arranged at the lofting frame, and an inlet for taking and putting the lofting frame is formed in the top end of the liquid nitrogen tank shell;
the liquid nitrogen replenishing bottle is arranged at one side of the liquid nitrogen tank shell, and a liquid replenishing pipe for liquid nitrogen replenishing is arranged between the liquid nitrogen replenishing bottle and the liquid nitrogen tank shell;
the lifting device is arranged in the inner container of the liquid nitrogen tank, and can reduce the loss of liquid nitrogen in the inner container of the liquid nitrogen tank in the process of taking and placing the lofting frame;
the lifting device comprises a placing seat, a supporting plate and an electric telescopic column, wherein a plurality of groups of placing seats for placing the lofting frame are arranged in the liquid nitrogen tank inner container, the supporting plates are arranged at the positions of the placing seats in a sliding mode respectively, the upper surfaces of the supporting plates are propped against the lower surfaces of the lofting frames, the electric telescopic columns are arranged at the inner bottoms of the placing seats, and the top ends of the electric telescopic columns are connected with the lower surfaces of the supporting plates.
Preferably, the liquid nitrogen tank shell and the liquid nitrogen tank inner container are provided with rotating mechanisms for rotating and aligning the positions between the plurality of groups of placing seats and the inlets, the rotating mechanisms comprise connecting rods and connecting frames, the connecting rods are rotatably arranged at the liquid nitrogen tank shell, one ends of the connecting rods extending to the inside of the liquid nitrogen tank inner container are provided with the connecting frames, and the plurality of groups of placing seats are connected with the connecting frames.
Preferably, the connecting rod is located the one end surface on liquid nitrogen container casing upper portion and is provided with the locating plate corresponding with the multiunit seat position of placing, multiunit the upper surface of locating plate all is provided with the label that is used for carrying out the mark to stem cell type.
Preferably, the upper surface of liquid nitrogen container casing is provided with the fixed shell, and the one end of fixed shell cup joints the surface at the connecting rod, and the multiunit the one end upper surface of locating plate all is provided with a set of locating screw, the constant head tank that is used for locating screw location grafting is seted up to the one end upper surface that the fixed shell corresponds with the locating screw.
Preferably, the inside of fixed shell is provided with the fixed establishment who is used for fixing connecting rod and link, fixed establishment includes electric telescopic handle, tooth piece and gear, the gear sets up the surface at the connecting rod, the inside of fixed shell is provided with electric telescopic handle, electric telescopic handle's one end is provided with the tooth piece, the one end of tooth piece meshes with the surface of gear.
Preferably, a control switch for driving the electric telescopic rod to stretch is arranged in the positioning groove, the bottom end of the positioning screw rod corresponds to the control switch, a driver controlled by the control switch is arranged at one end, away from the tooth block, of the electric telescopic rod, and the driver is arranged in the fixing shell.
Preferably, limiting plates are arranged on two sides of the supporting plate, limiting grooves used for limiting sliding of the limiting plates are formed on two sides of the placing seat, thread grooves are formed in the upper surface of the supporting plate, a fixing screw in threaded connection with the thread grooves is rotationally arranged at the lofting frame, and a sealing ring is arranged at one end, close to the inlet, of the placing seat.
Preferably, a sealing cover is arranged outside the inlet, and a sealing liner is arranged between the sealing cover and the inlet.
Preferably, an inner cavity for storing liquid nitrogen is arranged between the liquid nitrogen tank shell and the liquid nitrogen tank liner, a master controller is arranged at the top end of the liquid nitrogen tank shell, a liquid level detector is arranged at the master controller, and one end, far away from the master controller, of the liquid level detector extends to the inner portion of the inner cavity.
Preferably, a control valve for controlling liquid nitrogen replenishment is arranged at the top end of the liquid nitrogen replenishment bottle, and one end of the liquid replenishment pipe extends to the inner part of the inner cavity.
The invention discloses a low-temperature stem cell preservation device, which has the following beneficial effects:
according to the invention, the small-caliber inlet is formed in the liquid nitrogen tank, and the lifting mechanism is arranged in the liquid nitrogen tank liner, so that when the storage rack storing stem cells is taken or placed, the storage rack storing stem cells can be assisted by the lifting mechanism, in the process, the supporting plate and the sealing ring at the lifting mechanism can seal the inlet in the process of taking or installing the storage rack, on one hand, liquid nitrogen in the liquid nitrogen tank is effectively reduced from flowing into the external environment, and on the other hand, mediums in the external environment are prevented from entering the liquid nitrogen tank, so that the storage environment of the stem cells is effectively protected.
According to the invention, the rotating mechanism is arranged at the liquid nitrogen tank, and can drive the multiple groups of placing seats to rotate in a positioning way, so that when the storage rack is taken or placed, the corresponding group of placing seats can be precisely rotated to the inlet, the storage rack can be stably placed at the group of placing seats, and only one group of placing seats are rotated to the inlet under one-time rotation operation, so that the exposed caliber of the tank opening can be effectively reduced, and further the loss of liquid nitrogen in the liquid nitrogen tank is reduced.
When the liquid nitrogen filled in the inner cavity is gradually consumed, namely the liquid nitrogen level in the inner cavity gradually drops and reaches the lowest limit value of the liquid level detector, the liquid level detector can transmit the information to the master controller, and after receiving the information, the master controller can control the control valve at the liquid nitrogen replenishing bottle to be opened, so that the liquid nitrogen in the liquid nitrogen replenishing bottle is replenished into the inner cavity through the liquid replenishing pipe, the liquid nitrogen amount in the liquid nitrogen tank is always sufficient, and the long-term low-temperature storage requirement of stem cells is met.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a cross-sectional view of the shell and liner of the liquid nitrogen tank of the present invention;
FIG. 3 is an enlarged view of the structure of FIG. 2A in accordance with the present invention;
FIG. 4 is an exploded view of the inlet, bladder and seal cover configuration of the present invention;
FIG. 5 is a sectional view of the internal structure of the liquid nitrogen tank of the invention;
FIG. 6 is a schematic diagram of the structure of the loft frame of the present invention;
FIG. 7 is a schematic view of the structure of the placement base and the connecting frame of the present invention;
FIG. 8 is an enlarged view of the structure of FIG. 7B in accordance with the present invention;
FIG. 9 is an exploded view of the construction of the loft stand, support plate and set screw of the present invention;
FIG. 10 is a schematic view of a connecting rod and positioning plate structure of the present invention;
FIG. 11 is a sectional view showing the internal structure of the fixing case of the present invention;
fig. 12 is an enlarged view of the structure of fig. 11C according to the present invention.
In the figure: 1. a liquid nitrogen tank shell; 101. a liquid nitrogen tank liner; 102. an inner cavity; 103. an inlet; 104. sealing the liner; 105. sealing cover; 2. a liquid nitrogen replenishing bottle; 201. a fluid supplementing pipe; 202. a control valve; 203. a master controller; 204. a liquid level detector; 3. a lofting frame; 301. a drawer; 302. a fixed screw; 401. a placement seat; 402. a support plate; 403. an electric telescopic column; 404. a limiting plate; 405. a limit groove; 406. a thread groove; 407. a seal ring; 501. a connecting rod; 502. a connecting frame; 503. a positioning plate; 504. positioning a screw; 505. a fixed case; 506. a positioning groove; 601. a control switch; 602. a driver; 603. an electric telescopic rod; 604. tooth blocks; 605. a gear; 7. and (5) a label.
Detailed Description
The embodiment of the invention discloses a low-temperature stem cell preservation device, as shown in fig. 1-12, in order to make the purposes, technical schemes and advantages of the embodiment of the invention more clear, the technical schemes of the embodiment of the invention are clearly and completely described below by way of example with reference to the accompanying drawings in the invention.
Embodiment one:
as shown in fig. 1 to 12, a stem cell cryopreservation apparatus includes: the liquid nitrogen tank comprises a liquid nitrogen tank shell 1, a liquid nitrogen tank liner 101 and a lofting frame 3 arranged inside the liquid nitrogen tank liner 101, wherein a drawer 301 for independently storing a plurality of groups of stem cells is arranged at the lofting frame 3, an inlet 103 for taking and placing the lofting frame 3 is formed in the top end of the liquid nitrogen tank shell 1, a sealing cover 105 is arranged outside the inlet 103, a sealing liner 104 is arranged between the sealing cover 105 and the inlet 103, and the liquid nitrogen tank consists of the liquid nitrogen tank shell 1, the liquid nitrogen tank liner 101, an inner cavity 102, the inlet 103, the sealing liner 104 and the sealing cover 105.
The liquid nitrogen replenishing bottle 2 is arranged on one side of the liquid nitrogen tank shell 1, a liquid replenishing pipe 201 for liquid nitrogen replenishing is arranged between the liquid nitrogen replenishing bottle 2 and the liquid nitrogen tank shell 1, an inner cavity 102 for liquid nitrogen storage is arranged between the liquid nitrogen tank shell 1 and the liquid nitrogen tank inner container 101, a control valve 202 for liquid nitrogen replenishing control is arranged at the top end of the liquid nitrogen replenishing bottle 2, and one end of the liquid replenishing pipe 201 extends to the inner portion of the inner cavity 102.
The top of liquid nitrogen container casing 1 is provided with total controller 203, and total controller 203 department is provided with liquid level detector 204, and the one end that total controller 203 was kept away from to liquid level detector 204 extends to the inside of inner chamber 102, and total controller 203 can control liquid level detector 204 and control valve 202.
The inside of liquid nitrogen container inner bag 101 is provided with elevating gear, elevating gear can be at the in-process of getting the lofting frame 3 put the loss of liquid nitrogen container inner bag 101 inside liquid nitrogen, elevating gear is including placing seat 401, backup pad 402 and electric telescopic column 403, the bore of placing seat 401 is identical with the bore of import 103, the inside of liquid nitrogen container inner bag 101 is provided with the seat 401 that the multiunit was used for lofting frame 3 to place, multiunit backup pad 402 slides the setting respectively in seat 401 department is placed to the multiunit, and the upper surface of backup pad 402 offsets with the lower surface of lofting frame 3, the inner bottom of multiunit seat 401 all is provided with electric telescopic column 403, and the top of electric telescopic column 403 is connected with the lower surface of backup pad 402, through the electric telescopic column 403 that sets up, backup pad 402 that can promote to place seat 401 department carries out the upper and lower slip, when backup pad 402 moves to the highest spacing department of placing seat 401, backup pad 402 and the seal ring 407 that places the top of seat 401 can keep up import 103, be in the state between the two parts environment about backup pad 402, effectively reduce the loss of liquid nitrogen container inside.
The liquid nitrogen tank shell 1 and the liquid nitrogen tank inner container 101 are provided with rotating mechanisms for adjusting the positions of the plurality of groups of placing seats 401 so as to align the positions between the placing seats 401 and the inlets 103, each rotating mechanism comprises a connecting rod 501 and a connecting frame 502, the connecting rod 501 is rotatably arranged at the liquid nitrogen tank shell 1, one end of the connecting rod 501 extending into the liquid nitrogen tank inner container 101 is provided with the connecting frame 502, and the plurality of groups of placing seats 401 are connected with the connecting frame 502;
the connecting rod 501 is located the one end surface on liquid nitrogen container casing 1 upper portion and is provided with the locating plate 503 corresponding with multiunit seat 401 position, multiunit locating plate 503's upper surface all is provided with the label 7 that is used for carrying out the mark to stem cell type, a set of locating plate 503 corresponds with the setting position of a set of lofting frame 3, through the label 7 that locating plate 503 department set up, can be convenient fix a position the save position of different types stem cell, and when rotating a set of seat 401 to import 103 department, because seat 401 is connected through connecting rod 501 with locating plate 503, can rotate locating plate 503 to earlier with fixed shell 505 set up a position corresponding of constant head tank 506, thereby can make to place between lofting frame 3 and the import 103 of seat 401 department align accurately.
The upper surface of liquid nitrogen container casing 1 is provided with fixed shell 505, and the one end of fixed shell 505 cup joints the surface at connecting rod 501, and the one end upper surface of multiunit locating plate 503 all is provided with a set of locating screw 504, and fixed shell 505 and locating screw 504 corresponding one end upper surface have been seted up and are used for locating screw 504 location grafting's constant head tank 506, and the one end that fixed shell 505 seted up constant head tank 506 is just corresponding with the position of import 103.
The fixing mechanism for fixing the connecting rod 501 and the connecting frame 502 is arranged inside the fixing shell 505, the fixing mechanism comprises an electric telescopic rod 603, a tooth block 604 and a gear 605, the gear 605 is arranged on the outer surface of the connecting rod 501, the electric telescopic rod 603 is arranged inside the fixing shell 505, one end of the electric telescopic rod 603 is provided with the tooth block 604, the extendable length of the electric telescopic rod 603 is fixed, when the extendable length of the electric telescopic rod 603 extends to the maximum length, the tooth block 604 is meshed with the gear 605 at the moment, one end of the tooth block 604 is meshed with the outer surface of the gear 605,
the inside of constant head tank 506 is provided with the control switch 601 that is used for driving electric telescopic handle 603 to stretch out and draw back, correspond between the bottom of positioning screw 504 and the control switch 601, the one end that electric telescopic handle 603 kept away from tooth piece 604 is provided with the driver 602 of control switch 601 control, driver 602 sets up in the inside of fixed shell 505, after the one end grafting of positioning screw 504 is in the inside of positioning slot 506, can press the control switch 601 that positioning slot 506 inside set up, make control switch 601 open, after the one end of positioning screw 504 is removed from the inside of positioning slot 506, control switch 601 resets, control switch 601 closes, and then make the driver 602 that is controlled by control switch 601 closed, electric telescopic handle 603 contracts under the control of driver 602 this moment, make tooth piece 604 and the gear 605 between meshing of electric telescopic handle 603 one end, and then make the connecting rod 501 that is provided with gear 605 be in the active state, so that continuous rotation connecting rod 501 adjusts the position of other places 401.
Limiting plates 404 are arranged on two sides of the supporting plate 402, limiting grooves 405 for limiting sliding of the limiting plates 404 are formed on two sides of the placing seat 401, screw grooves 406 are formed on the upper surface of the supporting plate 402, a fixing screw 302 in threaded connection with the screw grooves 406 is rotatably arranged at the lofting frame 3, a sealing ring 407 is arranged at one end, close to the inlet 103, of the placing seat 401, and the inlet 103 can be further sealed through the sealing effect of the sealing ring 407.
In summary, before the stem cell cryopreservation apparatus is used, the master controller 203 is firstly controlled, so that the master controller 203 opens the control valve 202 disposed at the top of the liquid nitrogen replenishment bottle 2, so that the liquid nitrogen replenishment bottle 2, the liquid replenishing pipe 201, and the inner cavity 102 between the liquid nitrogen tank shell 1 and the liquid nitrogen tank inner container 101 are in a communication state, so that liquid nitrogen in the liquid nitrogen replenishment bottle 2 enters the inner cavity 102 along the liquid replenishing pipe 201, and in this process, the liquid level detector 204 located in the inner cavity 102 can monitor the replenishment amount of the liquid nitrogen in real time.
Further, after the interior of the inner cavity 102 is filled with suitable liquid nitrogen, that is, when the liquid nitrogen amount reaches the maximum limit value of the liquid level detector 204, the liquid level detector 204 may transmit the information to the master controller 203 connected with the liquid level detector, so that the master controller 203 controls the control valve 202 to be closed, and further the inner cavity 102 between the liquid nitrogen replenishing bottle 2, the liquid replenishing pipe 201 and the liquid nitrogen tank shell 1 and the liquid nitrogen tank liner 101 is in a non-communication state, so as to complete the operation of replenishing the liquid nitrogen into the inner cavity 102.
Further, after the liquid nitrogen is supplied into the inner cavity 102, the liquid nitrogen tank shell 1 can avoid the low temperature loss of the liquid nitrogen, and the low temperature of the liquid nitrogen can penetrate into the inner container 101 of the liquid nitrogen tank, so that the inner container 101 of the liquid nitrogen tank is in a low temperature state.
Further, after the inside of the liquid nitrogen tank is in a low temperature state, the connecting rod 501 at the top end of the liquid nitrogen tank shell 1 can be rotated at this time, that is, the locating plate 503 arranged at the top end of the connecting rod 501 is held, the locating screw 504 arranged at the locating plate 503 is rotated to a position corresponding to the locating slot 506 arranged at the fixing shell 505, that is, at this time, the group of placing seats 401 at the connecting frame 502 is rotated to a position corresponding to the inlet 103, and then the locating screw 504 arranged at the locating plate 503 can be rotated, so that one end of the locating screw 504 moves to the inside of the locating slot 506.
Further, after one end of the positioning screw 504 moves to the inside of the positioning groove 506, at this time, one end of the positioning screw 504 contacts with the control switch 601 disposed inside the positioning groove 506, and under the pressure of the positioning screw 504, the control switch 601 is turned on, after the control switch 601 is turned on, the driver 602 controlled by the control switch 601 is turned on, and then the driver 602 drives the electric telescopic rod 603 disposed at the driver 602 to extend, so that under the pushing of the electric telescopic rod 603, the tooth block 604 disposed at one end of the electric telescopic rod 603 moves to the gear 605 disposed near the connecting rod 501, and after the tooth block 604 is meshed with the gear 605, the connecting rod 501, the connecting frame 502 and the plurality of groups of placement seats 401 are fixed.
Further, after the fixing of the placement seat 401 is completed, the sealing cover 105 and the sealing liner 104 disposed at the inlet 103 can be opened successively at this time, so that the inlet 103 and a group of placement seats 401 corresponding to the group of inlets 103 are exposed, and at this time, the electric telescopic column 403 disposed at the bottom of the placement seat 401 can be started by the remote control device, so that the supporting plate 402 disposed at the top end of the electric telescopic column 403 moves upwards in a limited manner along the placement seat 401 under the pushing action of the electric telescopic column 403.
Further, when the support plate 402 moves to a position close to the inlet 103, the seal ring 407 arranged at the top end of the support plate 402 and the placing seat 401 can isolate the internal and external environments of the inlet 103, so that loss of liquid nitrogen in the liquid nitrogen tank liner 101 is reduced, then the lofting frame 3 with stem cells placed in the internal drawer 301 can be placed at the position of the support plate 402, and then the fixing screw 302 arranged at the top end of the lofting frame 3 can be rotated, so that one end of the fixing screw 302 is in threaded connection with the inside of the thread groove 406 formed in the surface of the support plate 402, and therefore the lofting frame 3 and the support plate 402 are connected and fixed.
Further, after the connection between the lofting frame 3 and the supporting plate 402 is completed, the electric telescopic column 403 arranged at the bottom of the placement seat 401 can be started again through the remote control device, so that under the action of the electric telescopic column 403, the supporting plate 402 arranged at the top end of the electric telescopic column 403 and the lofting frame 3 arranged at the supporting plate 402 move downwards along the placement seat 401, and after the lofting frame 3 moves completely to the inside of the placement seat 401, the remote control device can be closed, so that the electric telescopic column 403 stops shrinking.
In addition, when the electric telescopic column 403 is started to drive the support plate 402 to move, the limiting plates 404 arranged on two sides of the support plate 402 can slide in a limiting manner along the limiting grooves 405 formed on the side surface of the placement seat 401, so that the moving track of the support plate 402 is stable.
Further, the above operations may be repeated, and the sets of placing holders 401, on which the lofting holders 3 are not placed, are rotated to positions corresponding to the inlets 103, respectively, so that the lofting holders 3, on which the stem cells are placed, are placed inside the liquid nitrogen tank liner 101 through the placing holders 401 to preserve the plurality of sets of stem cells at a low temperature, then the sealing liner 104 is placed at the inlets 103 again, and then the sealing cover 105 is covered at the inlets 103 provided with the sealing liner 104, thereby increasing the tightness at the inlets 103.
Further, the label 7 may be attached to the upper surface of the positioning plate 503, so that different stem cells may be labeled, and when the stem cells are taken in the later stage, the stem cells may be located and found conveniently according to the label 7.
Finally, when the liquid nitrogen filled in the inner cavity 102 is gradually consumed, that is, the liquid level of the liquid nitrogen in the inner cavity 102 gradually decreases and reaches the lowest limit value of the liquid level detector 204, the liquid level detector 204 can transmit the information to the master controller 203, after receiving the information, the master controller 203 can control the control valve 202 at the liquid nitrogen replenishing bottle 2 to be opened, so that the liquid nitrogen in the liquid nitrogen replenishing bottle 2 is replenished into the inner cavity 102 through the liquid replenishing pipe 201, the liquid nitrogen amount in the liquid nitrogen tank is always sufficient, and the long-term low-temperature storage requirement of stem cells is met.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A stem cell cryopreservation apparatus, characterized in that: comprising the following steps:
the device comprises a liquid nitrogen tank shell (1), a liquid nitrogen tank liner (101) and a lofting frame (3) arranged inside the liquid nitrogen tank liner (101), wherein drawers (301) for independently storing a plurality of groups of stem cells are arranged at the lofting frame (3), and an inlet (103) for taking and putting the lofting frame (3) is formed in the top end of the liquid nitrogen tank shell (1);
the liquid nitrogen replenishing bottle (2) is arranged at one side of the liquid nitrogen tank shell (1), and a liquid replenishing pipe (201) for liquid nitrogen replenishing is arranged between the liquid nitrogen replenishing bottle (2) and the liquid nitrogen tank shell (1);
the lifting device is arranged in the liquid nitrogen tank liner (101), and can reduce the loss of liquid nitrogen in the liquid nitrogen tank liner (101) in the process of taking and placing the lofting frame (3); the lifting device comprises a placement seat (401), a support plate (402) and electric telescopic columns (403), wherein a plurality of groups of placement seats (401) used for placing the lofting frame (3) are arranged in the liquid nitrogen tank liner (101), the plurality of groups of support plates (402) are respectively arranged at the positions of the plurality of groups of placement seats (401) in a sliding mode, the upper surfaces of the support plates (402) are propped against the lower surfaces of the lofting frame (3), the electric telescopic columns (403) are arranged at the inner bottoms of the plurality of groups of placement seats (401), and the top ends of the electric telescopic columns (403) are connected with the lower surfaces of the support plates (402);
the liquid nitrogen tank comprises a liquid nitrogen tank body (1) and a liquid nitrogen tank liner (101), wherein a rotating mechanism is arranged at the liquid nitrogen tank body (1) and used for rotationally aligning the positions between a plurality of groups of placing seats (401) and an inlet (103), the rotating mechanism comprises a connecting rod (501) and a connecting frame (502), the connecting rod (501) is rotationally arranged at the liquid nitrogen tank body (1), the connecting frame (502) is arranged at one end of the connecting rod (501) extending to the inside of the liquid nitrogen tank liner (101), and a plurality of groups of placing seats (401) are connected with the connecting frame (502);
the outer surface of one end of the connecting rod (501) positioned at the upper part of the liquid nitrogen tank shell (1) is provided with positioning plates (503) corresponding to the positions of a plurality of groups of placing seats (401), and the upper surfaces of the groups of positioning plates (503) are provided with labels (7) for marking stem cell types;
the upper surface of the liquid nitrogen tank shell (1) is provided with a fixed shell (505), one end of the fixed shell (505) is sleeved on the outer surface of the connecting rod (501), one end upper surfaces of the positioning plates (503) are provided with a group of positioning screws (504), and one end upper surface of the fixed shell (505) corresponding to the positioning screws (504) is provided with a positioning groove (506) for positioning and inserting the positioning screws (504);
the fixing mechanism for fixing the connecting rod (501) and the connecting frame (502) is arranged in the fixing shell (505), the fixing mechanism comprises an electric telescopic rod (603), a tooth block (604) and a gear (605), the gear (605) is arranged on the outer surface of the connecting rod (501), the electric telescopic rod (603) is arranged in the fixing shell (505), a tooth block (604) is arranged at one end of the electric telescopic rod (603), and one end of the tooth block (604) is meshed with the outer surface of the gear (605);
the inside of constant head tank (506) is provided with control switch (601) that are used for driving electric telescopic handle (603) and carry out the flexible, correspond between the bottom of positioning screw (504) and control switch (601), one end that tooth piece (604) was kept away from to electric telescopic handle (603) is provided with driver (602) of control switch (601) control, driver (602) set up in the inside of fixed shell (505).
2. The cryopreservation apparatus for stem cells of claim 1, wherein: limiting plates (404) are arranged on two sides of the supporting plate (402), limiting grooves (405) used for limiting the sliding of the limiting plates (404) are formed on two sides of the placing seat (401), screw grooves (406) are formed in the upper surface of the supporting plate (402), fixing screws (302) in threaded connection with the screw grooves (406) are rotatably arranged at the lofting frame (3), and sealing rings (407) are arranged at one ends, close to the inlets (103), of the placing seat (401).
3. The cryopreservation apparatus for stem cells of claim 1, wherein: the outside of import (103) is provided with sealed lid (105), be provided with sealed courage (104) between sealed lid (105) and import (103).
4. The cryopreservation apparatus for stem cells of claim 1, wherein: an inner cavity (102) for storing liquid nitrogen is arranged between the liquid nitrogen tank shell (1) and the liquid nitrogen tank inner container (101), a main controller (203) is arranged at the top end of the liquid nitrogen tank shell (1), a liquid level detector (204) is arranged at the main controller (203), and one end, far away from the main controller (203), of the liquid level detector (204) extends to the inner portion of the inner cavity (102).
5. The cryopreservation apparatus for stem cells of claim 1, wherein: the top of the liquid nitrogen replenishing bottle (2) is provided with a control valve (202) for liquid nitrogen replenishing control, and one end of the liquid replenishing pipe (201) extends to the inside of the inner cavity (102).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310629636.1A CN116369309B (en) | 2023-05-31 | 2023-05-31 | Stem cell low-temperature preservation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310629636.1A CN116369309B (en) | 2023-05-31 | 2023-05-31 | Stem cell low-temperature preservation device |
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| CN116369309A CN116369309A (en) | 2023-07-04 |
| CN116369309B true CN116369309B (en) | 2023-08-11 |
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| CN202310629636.1A Active CN116369309B (en) | 2023-05-31 | 2023-05-31 | Stem cell low-temperature preservation device |
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|---|---|
| CN116369309A (en) | 2023-07-04 |
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