CN115975790A - Antigen expanding culture preserving device and method thereof - Google Patents

Abstract

The invention provides an antigen expansion and storage device and a method thereof, which belong to the field of antigen expansion and storage technology. The antigen expansion and storage device includes a cabinet, a storage mechanism and a cap screwing mechanism. The cabinet has an accommodating cavity; the The storage mechanism is arranged in the accommodating cavity, the storage mechanism includes a storage box, the storage box has an accommodating cavity and an inner groove, the accommodating cavity is used for installing cold and heat regulating equipment, and the inner groove There are storage cylinders distributed along the circumferential direction inside, each of which is equipped with a storage bucket, and a first constant temperature cavity is formed between the storage bucket and the storage cylinder; The cold/hot air is input into the cavity, so that the cold/hot air surrounds the surface of the preservation bucket, and at the same time enters the second constant temperature cavity through the first side wall forming a mesh, so that part of the cold/hot air is concentrated in the second constant temperature cavity, The cold/hot air can flow in the second constant temperature cavity and surround the expansion test tubes to keep the constant temperature of the expansion test tubes.

Description

Antigen expansion and storage device and method thereof

technical field

The invention belongs to the technical field of antigen expansion and cultivation, and in particular relates to an antigen expansion and storage device and a method thereof.

Background technique

Expansion culture technology is often used in the process of antigen expansion. The use of high-density fermentation culture technology increases the expression of antigens, and at the same time ensures that the antigens are expressed in a soluble form, which reduces production costs and improves the quality of the final antigen. .

During the antigen expansion process, there are high requirements for the temperature environment of the expansion equipment. Although the current expansion device has a strong cooling and heating effect, it has a relatively wide space, which makes the air have better fluidity, resulting in the expansion device not being able to achieve a constant temperature effect, which is not conducive to the antigen. preservation.

Contents of the invention

The embodiment of the present invention provides an antigen expansion and storage device and its method, which aims to solve the problem that the existing expansion equipment is difficult to achieve constant temperature.

In view of the problems referred to above, the technical solution proposed by the present invention is:

In the first aspect, the present invention provides an antigen expansion and preservation device, comprising:

a cabinet, the cabinet has an accommodating cavity;

A storage mechanism, the storage mechanism is arranged in the storage cavity, the storage mechanism includes a storage box, the storage box has a storage cavity and an inner groove, and the storage cavity is used for installing cold and heat adjustment equipment, There are storage tubes distributed along the circumferential direction in the inner groove, each of the storage tubes is provided with a storage bucket, and a first constant temperature cavity is formed between the storage buckets and the storage tubes, and each of the storage tubes Shelves can be placed in the storage bucket, and when the storage rack is filled with expansion test tubes, a second constant temperature cavity is formed between a plurality of expansion test tubes and the storage bucket, and the second constant temperature cavity and The first constant temperature cavity is communicated, and each of the preservation barrels is closed by an upper cover;

Wherein, the preservation bucket has a first side wall, and the first side wall is mesh-shaped;

A cap screwing mechanism, the cap screwing mechanism is arranged along the circumferential direction of the preservation box.

As a preferred technical solution of the present invention, the accommodating chamber is provided with a rotating plate rotatable by a rotating shaft, and both the storage mechanism and the cap screwing mechanism are arranged on the rotating plate.

As a preferred technical solution of the present invention, a transmission part is connected to the rotating shaft, and the transmission part includes two runners and a transmission toothed belt, and the two runners are connected through the transmission toothed belt, one of which The rotating wheel is sheathed on the rotating shaft, and the other rotating wheel is in transmission connection with the first driving motor.

As a preferred technical solution of the present invention, the accommodating cavity is provided with an injection assembly on the opposite surface of the inner groove, the injection assembly includes a moving part and the injection part, and the injection part and the moving Component connections.

As a preferred technical solution of the present invention, the moving part includes a first guide rail, a first slider is provided on the first guide rail, a connection block is provided on the first slider, and a connection block is connected to the connection block. There is a screw connected to the output of the second drive motor.

As a preferred technical solution of the present invention, the injection part includes a delivery box and the delivery needle, the delivery box is connected to the connection block, and a plurality of delivery needles are distributed in a circular array on the delivery box.

As a preferred technical solution of the present invention, the cap screwing mechanism includes a back plate, a support plate, a lifting assembly, a driver, a crank, a mounting plate and a cap screw assembly, and a direction changing groove is opened on the back plate, and the back plate There are support plates in a linear array on the board, the lifting components are arranged between the support plates, the telescopic end of the driver is connected with the lifting components, and one end of the crank can be movably connected to the In the direction changing slot, the mounting plate is connected to the other end of the crank, and the screw cap assembly is mounted on the mounting plate.

As a preferred technical solution of the present invention, the support plates arranged longitudinally form a group, the lifting assembly includes a second guide rail, a second slider and a moving plate, and the support plates of each group are provided with the The second guide rails, each of the second guide rails is provided with the second sliders, and the moving plate is arranged between the two second sliders.

As a preferred technical solution of the present invention, the cap screw assembly includes a fourth drive motor, a transmission shaft and a cap screw cylinder, the fourth drive motor is installed on the mounting plate, and one end of the drive shaft runs through The mounting plate is also connected to the output end of the fourth driving motor, and the cap screwing cylinder is arranged at the other end of the transmission shaft.

In another aspect, the present invention provides a method for antigen expansion and preservation, comprising the following steps:

S1, after placing the expansion test tubes on the shelf, a second constant temperature cavity is formed between the multiple expansion test tubes and the preservation bucket;

S2, after placing each preservation barrel in the preservation barrel, a first constant temperature cavity is formed between the preservation barrel and the preservation barrel, and the upper cover and the preservation barrel are closed by a cap screwing mechanism;

S3, the cold/heat regulating device inputs cold/hot air into the first constant temperature cavity, so that the cold/hot air surrounds the surface of the preservation bucket, and at the same time enters the second constant temperature cavity through the mesh-shaped first side wall, so that part of the cold /The hot air is concentrated in the second constant temperature cavity and surrounds between the expansion test tubes, so that the expansion test tubes can be kept at a constant temperature.

Compared with the prior art, the beneficial effects of the present invention are:

(1) After the rack is filled with expansion test tubes, a second constant temperature cavity is formed between the multiple expansion test tubes and the preservation bucket. After each preservation barrel is placed in the preservation barrel, a first constant temperature cavity is formed between the preservation barrel and the preservation barrel, and the upper cover and the preservation barrel are closed by the screw cap mechanism, so that the upper cover and the preservation barrel form a narrow seal space; at the same time, the cold and heat regulating equipment inputs cold/hot air into the first constant temperature cavity, so that the cold/hot air surrounds the surface of the preservation barrel, and at the same time enters the second constant temperature cavity through the first side wall forming a mesh, so that Part of the cold/hot air is concentrated in the second constant temperature cavity, allowing the cold/hot air to flow in the second constant temperature cavity and surround each expansion test tube to keep the temperature of each expansion test tube.

(2) The cap screwing mechanism includes a back plate, a support plate, a lifting assembly, a driver, a crank, a mounting plate and a screw cap assembly. The cap screwing mechanism can make the upper cover open and close with the preservation barrel, and then cannot be operated, which reduces labor intensity and provides convenience for the preservation of the expansion test tube.

The above description is only an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention, it can be implemented according to the contents of the description, and in order to make the above and other purposes, features and advantages of the present invention more obvious and understandable , the specific embodiments of the present invention are enumerated below.

Description of drawings

Figure 1 is a perspective view of an antigen expansion and preservation device disclosed in the present invention;

Fig. 2 is a top sectional view of an antigen expansion and preservation device disclosed in the present invention;

Fig. 3 is a structural schematic diagram of a transmission part of an antigen expansion and storage device disclosed in the present invention;

Fig. 4 is a structural schematic diagram of a preservation box of an antigen expansion and preservation device disclosed in the present invention;

Fig. 5 is a partial enlarged view of place A in Fig. 4;

Fig. 6 is a main sectional view of a storage box of an antigen expansion and storage device disclosed in the present invention;

Fig. 7 is a schematic structural view of a shelf of an antigen expansion and storage device disclosed in the present invention;

Fig. 8 is a schematic structural view of an injection component of an antigen expansion and storage device disclosed in the present invention;

Fig. 9 is a structural schematic diagram of a cap screwing mechanism of an antigen expansion and preservation device disclosed in the present invention;

Fig. 10 is a flow chart of an antigen expansion and preservation method disclosed in the present invention.

Explanation of reference signs: 100, cabinet; 101, double door; 102, operation surface; 110, accommodation chamber; 111, rotating shaft; 112, rolling groove; 120, rotating plate; 121, connection hole; 130, first driving motor ; 140, transmission part; 141, transmission wheel; 142, transmission toothed belt; 150, injection assembly; 151, moving part; 1511, first guide rail; 1512, first slider; , the second drive motor; 152, the injection part; 1521, the delivery box; 1522, the delivery needle; 160, the support rod; 161, the ball; 200, the preservation mechanism; 210, the preservation box; Groove; 213, connection cavity; 220, preservation cylinder; 221, first constant temperature cavity; 222, first protrusion; 230, preservation bucket; 231, second constant temperature cavity; 232, first side wall; 233, Upper cover; 2321, second protrusion; 240, shelf; 241, storage hole; 250, rotating component; 251, first gear; 252, second gear; 253, third drive motor; 300, cap screwing mechanism; 310, backboard; 311, change direction slot; 320, support plate; 330, lifting assembly; 331, second guide rail; 332, second slider; 333, moving plate; 3331, through hole; 340, driver; 350, Crank; 351, limit plate; 360, mounting plate; 370, screw cap assembly; 371, fourth drive motor; 372, transmission shaft; 373, screw cap cylinder.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments It is some embodiments of the present invention, but not all of them. Based on the implementation manners in the present invention, all other implementation manners obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the invention. Based on the implementation manners in the present invention, all other implementation manners obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

It should be noted that like numerals and letters denote similar items in the following figures, therefore, once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", " Orientation indicated by rear, left, right, vertical, horizontal, top, bottom, inside, outside, clockwise, counterclockwise, etc. The positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, Therefore, it should not be construed as limiting the invention.

In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

Embodiment one

Referring to the accompanying drawings 1 to 9, the present invention provides a technical solution: an antigen expansion and storage device, including a cabinet 100, a storage mechanism 200 and a capping mechanism 300, the storage mechanism 200 can be located in the cabinet 100, and can be used for storage The mechanism 200 provides a storage space; the storage mechanism 200 is used to provide a constant temperature storage space for the expansion test tube, to ensure that the antigen is always at a suitable temperature state, and to avoid the failure of the expansion; The barrel 230 is opened and closed, thereby eliminating the need for manual operation, reducing labor intensity, and providing convenience for the preservation of the expansion test tube.

In this embodiment, the cabinet 100 has an accommodating cavity 110, and the storage mechanism 200 is arranged in the accommodating cavity 110. The storage mechanism 200 includes a storage box 210, and the storage box 210 has an accommodating cavity 211 and an inner groove 212. The accommodating cavity 211 is used to install the cooling and heating adjustment equipment. There are storage tubes 220 distributed along the circumferential direction in the inner groove 212. Each storage tube 220 is provided with a storage bucket 230, and a second storage bucket 230 is formed between the storage buckets 230 and the storage tubes 220. A constant temperature cavity 221, each storage barrel 230 can place a shelf 240, when the shelf 240 is filled with expansion test tubes, a second constant temperature cavity 231 is formed between a plurality of expansion test tubes and the preservation barrel 230 , the second constant temperature cavity 231 communicates with the first constant temperature cavity 221, and each preservation barrel 230 is closed by an upper cover 233, and the upper cover 233 is threadedly connected with the preservation barrel 230; wherein, the preservation barrel 230 has a first side wall 232, The first side wall 232 is net-shaped; the cap screwing mechanism 300 is arranged along the circumferential direction of the storage box 210 .

Exemplarily, after the storage rack 240 is filled with expansion test tubes, a second constant temperature cavity 231 is formed between the plurality of expansion test tubes and the preservation bucket 230 . After each preservation barrel 230 is placed in the preservation barrel 220, a first constant temperature cavity 221 is formed between the preservation barrel 230 and the preservation barrel 220, and the upper cover 233 and the preservation barrel 220 are covered by the screw cap mechanism 300, so that the upper The lid 233 and the preservation barrel 230 form a narrow closed space (i.e. the second constant temperature cavity 231); at the same time, the cooling and heating equipment inputs cold/hot air into the first constant temperature cavity 221, so that the cold/hot air surrounds the preservation barrel 230. At the same time, it enters the second constant temperature cavity 231 through the first side wall 232 forming a mesh, so that part of the cold/hot air is concentrated in the second constant temperature cavity 231, and the cold/hot air can be used in the second constant temperature cavity 231 It flows and surrounds each expansion test tube to keep each expansion test tube at a constant temperature.

In an optional embodiment, the bottom of each storage tube 220 is respectively provided with an air intake hole and an exhaust hole, and the cooling and heating adjustment device communicates with the air intake hole and the exhaust hole respectively through two conduits. On the one hand, the cold/hot air circulation in the storage cylinder 220 and the storage bucket 230 can be realized; on the other hand, the circulation of the cold/hot air can keep the pressure of the storage cylinder 220 and the storage bucket 230 in balance with the outside.

In an optional embodiment, the cabinet 100 is provided with a hinged double door 101 , and the gaps between the double door 101 and the cabinet 100 can be sealed by sealing strips, so that the accommodating cavity 110 can also form a sealed space. Furthermore, the air flow in the interior is reduced, which helps the preservation box 210 to maintain a constant temperature.

In an optional embodiment, the cabinet 100 has a working surface. The cabinet 100 not only provides the storage function, but also can realize the function of the test platform.

In an optional embodiment, the accommodating chamber 110 is provided with a rotating plate 120 rotatable through a rotating shaft 111 , and the storage mechanism 200 and the cap screwing mechanism 300 are both disposed on the rotating plate 120 . The rotating shaft 111 provides the function of a shaft for the rotating plate 120. The two ends of the rotating shaft 111 are respectively connected to the top and the bottom of the cabinet 100. When the rotating plate 120 and the rotating shaft 111 are fixed, the rotating plate 120 can rotate clockwise.

Exemplarily, when the rotating shaft 111 is driven, the rotating shaft 111 drives the rotating plate 120 to rotate in an arc toward the outside of the cabinet 100, and the storage box 210 can be located outside the cabinet 100 for the staff to inject antigens into the expansion test tubes, etc. substance. After the injection is completed, the rotating shaft 111 can drive the rotating plate 120 to return to the initial position again.

In an optional embodiment, the transmission component 140 may be a transmission between gear sets. In the embodiment of the present invention, the rotating shaft 111 is connected with a transmission part 140, and the transmission part 140 includes two runners and a transmission toothed belt 142, and the two runners are connected by transmission through the transmission toothed belt 142, and one of the runners is sheathed On the rotating shaft 111 , another rotating wheel is in drive connection with the first driving motor 130 . The transmission part 140 can drive the rotation shaft 111 to rotate, so as to rotate the rotating plate 120 in or out.

Exemplarily, after the first driving motor 130 is started, it drives one of its wheels to rotate, and through the action of the transmission toothed belt 142 , the two wheels can be rotated together, thereby driving the rotating shaft 111 .

In an optional embodiment, the accommodating cavity 110 is located on the opposite surface of the inner groove 212 to provide an injection assembly 150 , the injection assembly 150 includes a moving part 151 and an injection part 152 , and the injection part 152 is connected to the moving part 151 . The injection component 150 can inject biological culture substances into the expansion test tube without opening the double door 101, so that the growth of the antigen has enough nutrients.

In an optional embodiment, the moving part 151 includes a first guide rail 1511, a first slider 1512 is arranged on the first guide rail 1511, a connecting block 1513 is arranged on the first slider 1512, and a screw rod is connected to the connecting block 1513 1514 , the screw 1514 is connected to the output end of the second drive motor 1515 . Driven by the second drive motor 1515 , the first slider 1512 moves along the first guide rail 1511 , so that the position of the injecting component 152 can be adjusted.

Exemplarily, the center of the connection block 1513 has a screw hole, and the screw rod 1514 is threadedly connected with the screw hole. After the second driving motor 1515 is powered on, the screw rod 1514 is driven to rotate, so that the connecting block 1513 drives the first sliding block 1512 to move along the first guide rail 1511 .

In an optional embodiment, the injection part 152 includes a delivery box 1521 and delivery needles 1522, the delivery box 1521 is connected to the connection block 1513, and a plurality of delivery needles 1522 are distributed in a circular array on the delivery box 1521. Part of the nutrient solution can be stored in the delivery box 1521. When the nutrient solution needs to be injected, the valve between the delivery needle 1522 and the delivery box 1521 can be opened, and the nutrient solution can be injected into the culture test tube through the delivery needle 1522.

In an optional embodiment, the rotating plate 120 is provided with a connecting hole 121, and the connecting hole 121 has a through support rod 160, the supporting rod 160 is fixed to the rotating plate 120, and both ends of the supporting rod 160 are provided with balls 161, The top and the bottom of the accommodating cavity 110 are provided with rolling grooves 112 for the balls 161 to move in an arc. The support rod 160 is used to provide support for the rotating plate 120 during the rotation process of the rotating plate 120, so as to ensure that the rotating plate 120 can be rotated.

Exemplarily, the diameter of the rolling groove 112 is slightly smaller than that of the ball 161 , so that the rolling groove 112 just clamps a third of the ball 161 . When the rotating plate 120 is rotated, the rolling groove 112 not only restricts the ball 161 , but also enables the support rod 160 to move in an arc around the rolling groove 112 through the ball 161 .

In an optional embodiment, the inner wall of the preservation barrel 230 is provided with opposite first protrusions 222 , and the preservation barrel 230 is provided with a first groove matching the first protrusions 222 . The combined effect of the first groove and the first protrusion 222 can prevent the preservation barrel 230 from being driven to rotate during the process of opening and closing the upper cover 233 by the tightening device.

In an optional embodiment, a plurality of storage holes 241 are distributed in a circular array on the storage rack 240 . The storage hole 241 can be used for the expansion test tube to be inserted into the preservation barrel 230 , and the edge portion of the storage hole 241 can provide support for the expansion test tube, so that the expansion test tube and the preservation barrel 230 form a second constant temperature cavity 231 .

In an optional embodiment, a rotating part 250 is provided between the preservation barrel 230 and the rotating plate 120, the rotating part 250 includes a first gear 251, a second gear 252 and a third driving motor 253, and the first gear 251 is fixed on On the rotating plate 120 , the second gear 252 meshes with the first gear 251 , the output end of the third driving motor 253 is connected with the second gear 252 , and the third driving motor 253 is installed on the preservation barrel 230 . The third driving motor 253 drives the second gear 252 to rotate, and can transmit to the first gear 251 to drive the preservation bucket 230 to move in a circumferential direction.

Exemplarily, during the process of injecting the nutrient solution, the storage cylinder 220 to be injected is first rotated to the cap screwing mechanism 300 to uncap through the rotating member 250, and the rotating member 250 rotates the storage barrel 230 again so that the storage cylinder 220 to be injected is located at The nutrient solution is injected at the injection part 250; and after completion, the rotating part 250 finally rotates the preservation barrel 230 to rotate to the cap screwing mechanism 300 for capping.

In an optional embodiment, the preservation bucket 230 is provided with a connection chamber 213, and the rotating plate 120 can be bearing-connected to the connection chamber 213 through a support column. It can make the preservation barrel 230 rotate more smoothly.

In an optional embodiment, the cap screwing mechanism 300 includes a back plate 310, a support plate 320, a lift assembly 330, a driver 340, a crank 350, a mounting plate 360, and a cap screw assembly 370, and a direction change slot is opened on the back plate 310 311, the back plate 310 is provided with a linear array of support plates 320, the lift assembly 330 is arranged between each support plate 320, the telescopic end of the driver 340 is connected with the lift assembly 330, and one end of the crank 350 can be movably connected in the direction change In the slot 311 , the mounting plate 360 is connected to the other end of the crank 350 , and the screw cap assembly 370 is mounted on the mounting plate 360 . The crank 350 is in a zigzag shape. In the embodiment of the present invention, the driver 340 is one of an air cylinder, a hydraulic cylinder or an electric push rod. The driver 340 pushes the lifting assembly 330 to make the mounting plate 360 linearly move along the height of the back plate 310 , and the crank 350 drives the mounting plate 360 to be parallel or perpendicular to the storage box 210 as the path of the direction changing groove 311 changes. Furthermore, when the mounting plate 360 moves upwards, a rotating space can be provided for the preservation bucket 230; when the mounting plate 360 moves downwards, the delivery needle 1522 can be inserted into the culture test tube to inject the nutrient solution.

Exemplarily, the driver 340 pushes the lifting assembly 330 to drive the mounting plate 360 to move upwards, and the crank 350 rotates with the change of the direction changing groove 311, so that the mounting plate 360 is perpendicular to the storage box 210, and the screw cover assembly 370 is connected to the storage box 210. keep parallel. In the same way, the driver 340 pushes the lifting assembly 330 to drive the mounting plate 360 to move downward, and the crank 350 rotates with the change of the direction changing groove 311, so that the mounting plate 360 is parallel to the storage box 210, and the screw cover assembly 370 is aligned with the storage box 210. Keep it vertical. And as the mounting plate 360 continues to descend, the delivery needle 1522 can be slowly inserted into the culture test tube.

In an optional embodiment, the moving plate 333 is provided with a through hole 3331 through which the crank 350 can pass. The crank 350 can freely rotate in the through hole 3331 , so that the crank 350 can drive the rotation of the mounting plate 360 .

In an optional embodiment, the end of the crank 350 close to the direction changing groove 311 clamps the back plate 310 through two limiting plates 351 , and there is a slight gap between the limiting plates 351 and the back plate 310 . When the crank 350 is moved, the limiting plate 351 plays a role of limiting the crank 350 to prevent the crank 350 from breaking away from the direction changing groove 311 .

In an optional embodiment, the support plates 320 arranged longitudinally form a group, and the lifting assembly 330 includes a second guide rail 331, a second slider 332 and a moving plate 333, and a second The guide rails 331 , each second guide rail 331 is provided with a second slider 332 , and the moving plate 333 is arranged between the two second sliders 332 . After the moving plate 333 is driven, the two second sliders 332 can move longitudinally along the length direction of the second guide rail 331 .

In an optional embodiment, the cap screw assembly 370 includes a fourth drive motor 371, a transmission shaft 372 and a cap screw cylinder 373, the fourth drive motor 371 is installed on the mounting plate 360, and one end of the drive shaft 372 passes through the mounting plate 360 and is connected with the output end of the fourth driving motor 371, and the cap screwing cylinder 373 is arranged on the other end of the transmission shaft 372. The fourth driving motor 371 can drive the transmission shaft 372 to rotate after starting, so that the cap screwing cylinder 373 can be driven by the transmission shaft 372, and the cap screwing cylinder 373 can realize the opening and closing of the upper cover 233.

In an optional embodiment, the upper cover 233 has an opposite second protrusion 2321 , and the cap screwing cylinder 373 has a second groove (not shown in the figure) matching the second protrusion 2321 . Therefore, during the process of applying force to the upper cover 233 by the cap screwing cylinder 373 , the cooperation of the second groove and the second protrusion 2321 facilitates the opening and closing of the upper cover 233 .

Embodiment two

Referring to Figure 10, an embodiment of the present invention further provides an antigen expansion and preservation method, which includes the following steps:

S1, after the storage rack 240 is filled with expansion test tubes, a second constant temperature cavity 231 is formed between the plurality of expansion test tubes and the preservation bucket 230;

S2, after placing each preservation barrel 230 in the preservation barrel 220, a first constant temperature cavity 221 is formed between the preservation barrel 230 and the preservation barrel 220, and the upper cover 233 and the preservation barrel 220 are covered by the cap screwing mechanism 300;

S3, the cold/heat regulating device inputs cold/hot air into the first constant temperature cavity 221, so that the cold/hot air surrounds the surface of the preservation bucket 230, and at the same time enters the second constant temperature cavity 231 through the first side wall 232 forming a mesh, Part of the cold/hot air is concentrated in the second constant temperature cavity 231 and surrounds each expansion test tube, so that each expansion test tube can be kept at a constant temperature.

It should be noted that the models and specifications of the first driving motor 130, the second driving motor 1515, the third driving motor 253, the fourth driving motor 371 and the driver 340 need to be selected according to the actual specifications of the device, etc. The calculation method adopts the existing technology in the field, so it will not be described in detail.

The power supply and principle of the first driving motor 130 , the second driving motor 1515 , the third driving motor 253 , the fourth driving motor 371 and the driver 340 are clear to those skilled in the art, and will not be described in detail here.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (10)

1. An antigen expanding culture preserving device, which is characterized by comprising:

a cabinet having an accommodation chamber;

the preservation mechanism is arranged in the containing cavity and comprises a preservation box, the preservation box is provided with a containing cavity and an inner groove, the containing cavity is used for installing cold and heat adjusting equipment, preservation cylinders distributed along the circumferential direction are arranged in the inner groove, a preservation barrel is arranged in each preservation cylinder, a first constant-temperature cavity is formed between each preservation barrel and each preservation cylinder, a storage rack can be placed in each preservation barrel, after the storage rack is filled with the expansion culture test tubes, a second constant-temperature cavity is formed between each expansion culture test tube and each preservation cylinder and is communicated with the first constant-temperature cavity, and each preservation cylinder is covered by an upper cover;

wherein the preservation barrel is provided with a first side wall which is net-shaped;

the screwing-cover mechanism is arranged along the circumferential direction of the storage box.

2. The antigen expanding culture preserving device according to claim 1, wherein the accommodating cavity is provided with a rotating plate which can rotate through a rotating shaft, and the preserving mechanism and the screwing mechanism are both arranged on the rotating plate.

3. The antigen expanding culture and preservation device according to claim 2, wherein the rotating shaft is connected with a transmission component, the transmission component comprises two rotating wheels and a transmission toothed belt, the two rotating wheels are in transmission connection through the transmission toothed belt, one rotating wheel is sleeved on the rotating shaft, and the other rotating wheel is in transmission connection with the first driving motor.

4. The antigen expanding culture preservation device according to claim 1, wherein an injection assembly is arranged on a face of the accommodating cavity opposite to the inner groove, the injection assembly comprises a moving part and the injection part, and the injection part is connected with the moving part.

5. The antigen expanding culture and preservation device according to claim 4, wherein the moving member comprises a first guide rail, the first guide rail is provided with a first sliding block, the first sliding block is provided with a connecting block, the connecting block is connected with a screw rod, and the screw rod is connected with an output end of the second driving motor.

6. The antigen expanding culture and preservation device according to claim 5, wherein the injection component comprises a delivery box and the delivery needles, the delivery box is connected with the connecting block, and a plurality of delivery needles are distributed on the delivery box in a circular array.

7. The antigen expanding culture preservation device according to claim 1, wherein the screw cap mechanism comprises a back plate, support plates, lifting components, a driver, a crank, a mounting plate and a screw cap component, the back plate is provided with turning grooves, the back plate is provided with the support plates in a linear array, the lifting components are arranged between the support plates, the telescopic end of the driver is connected with the lifting components, one end of the crank is movably connected in the turning grooves in a penetrating manner, the mounting plate is connected to the other end of the crank, and the screw cap component is mounted on the mounting plate.

8. The antigen expanding culture and preservation device according to claim 7, wherein the support plates are arranged in a group, the lifting assembly comprises second guide rails, second sliding blocks and a moving plate, the second guide rails are arranged between the support plates in each group, the second sliding blocks are arranged on the second guide rails, and the moving plate is arranged between the second sliding blocks.

9. The antigen expanding culture preserving device according to claim 8, wherein the screw cap assembly comprises a fourth driving motor, a transmission shaft and a screw cap cylinder, the fourth driving motor is mounted on the mounting plate, one end of the transmission shaft penetrates through the mounting plate and is connected with an output end of the fourth driving motor, and the screw cap cylinder is arranged at the other end of the transmission shaft.

10. An antigen expanding culture preserving method applied to an antigen expanding culture preserving device of claims 1-9, which is characterized by comprising the following steps:

s1, placing a plurality of expanding culture test tubes on a storage rack, and forming second constant-temperature cavities between the plurality of expanding culture test tubes and a storage barrel;

s2, after each preservation barrel is placed in the preservation barrel, a first constant-temperature cavity is formed between the preservation barrel and the preservation barrel, and the upper cover and the preservation barrel are covered by a cover screwing mechanism;

and S3, the cold/hot air is input into the first constant temperature cavity by the cold/hot adjusting equipment so as to surround the cold/hot air on the surface of the storage barrel, and simultaneously enters the second constant temperature cavity through the first side wall which forms a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity and surrounds among the expansion culture test tubes, and the expansion culture test tubes can be kept at constant temperature.

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