CN115975790A - Antigen expanding culture preserving device and method thereof - Google Patents

Abstract

The invention provides an antigen expanding culture preserving device and a method thereof, belonging to the technical field of antigen expanding culture, the antigen expanding culture preserving device comprises a cabinet, a preserving mechanism and a screw capping mechanism, wherein the cabinet is provided with an accommodating cavity; the preservation mechanism is arranged in the accommodating cavity and comprises a preservation box, the preservation box is provided with an accommodating cavity and an inner groove, the accommodating cavity is used for mounting cold and heat adjusting equipment, preservation cylinders distributed along the circumferential direction are arranged in the inner groove, a preservation barrel is arranged in each preservation cylinder, and a first constant temperature cavity is formed between the preservation barrels and the preservation cylinders; the cold/hot air is input into the first constant temperature cavity by the cold/hot air adjusting device, so that the cold/hot air surrounds the surface of the storage barrel, meanwhile, enters the second constant temperature cavity through the first side wall which is formed into a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity, the cold/hot air can flow in the second constant temperature cavity and surround the expansion culture test tubes, and the expansion culture test tubes can be kept at constant temperature.

Description

Antigen expanding culture preserving device and method thereof

Technical Field

The invention belongs to the technical field of antigen expanding culture, and particularly relates to an antigen expanding culture preserving device and an antigen expanding culture preserving method.

Background

The amplification culture technology is often used in the amplification culture process of the antigen, the high-density fermentation culture technology is used to improve the expression quantity of the antigen, and simultaneously, the antigen is ensured to be expressed in a soluble form, so that the effects of reducing the production cost and improving the final antigen quality are achieved.

In the process of antigen expanding culture, the temperature environment of the expanding culture device has higher requirements. Although the existing expanding culture device has stronger refrigerating and heating effects, the existing expanding culture device has wider space, so that the air has better fluidity, the constant-temperature effect cannot be well realized by the expanding culture device, and the preservation of antigens is not facilitated.

Disclosure of Invention

The embodiment of the invention provides an antigen expanding culture preserving device and a method thereof, aiming at solving the problem that the existing expanding culture device is difficult to realize constant temperature.

In view of the above problems, the technical solution proposed by the present invention is:

in a first aspect, the present invention provides an antigen expanding culture and preservation device, comprising:

a cabinet having an accommodating chamber;

the preservation mechanism is arranged in the containing cavity and comprises a preservation box, the preservation box is provided with a containing cavity and an inner groove, the containing cavity is used for installing cold and heat adjusting equipment, preservation cylinders distributed along the circumferential direction are arranged in the inner groove, a preservation barrel is arranged in each preservation cylinder, the preservation barrels and the preservation cylinders form a first constant temperature cavity, a storage rack can be placed in each preservation cylinder, after the storage rack is filled with the expansion culture test tubes, a second constant temperature cavity is formed between each expansion culture test tube and each preservation cylinder and is communicated with the first constant temperature cavity, and each preservation cylinder is covered by an upper cover;

the preservation barrel is provided with a first side wall, and the first side wall is net-shaped;

the screwing-cover mechanism is arranged along the circumferential direction of the storage box.

As a preferred technical scheme of the invention, the accommodating cavity is provided with a rotating plate which can rotate through a rotating shaft, and the preservation mechanism and the screw capping mechanism are both arranged on the rotating plate.

As a preferable technical scheme of the present invention, the rotating shaft is connected with a transmission component, the transmission component includes two rotating wheels and a transmission toothed belt, the two rotating wheels are in transmission connection through the transmission toothed belt, one of the rotating wheels is sleeved on the rotating shaft, and the other rotating wheel is in transmission connection with the first driving motor.

As a preferable technical solution of the present invention, an injection assembly is disposed on a surface of the accommodating cavity opposite to the inner groove, the injection assembly includes a moving part and the injection part, and the injection part is connected to the moving part.

As a preferable technical solution of the present invention, the moving part includes a first guide rail, a first slider is disposed on the first guide rail, a connecting block is disposed on the first slider, a screw is connected to the connecting block, and the screw is connected to an output end of the second driving motor.

As a preferable technical scheme of the invention, the injection component comprises a conveying box and the conveying needles, the conveying box is connected with the connecting block, and a plurality of conveying needles are distributed on the conveying box in a circular array.

As a preferred technical scheme of the present invention, the cap screwing mechanism includes a back plate, support plates, a lifting assembly, a driver, a crank, a mounting plate, and a cap screwing assembly, wherein a turning groove is formed in the back plate, the support plates are arranged in a linear array on the back plate, the lifting assembly is arranged between the support plates, a telescopic end of the driver is connected to the lifting assembly, one end of the crank is movably connected in the turning groove in a penetrating manner, the mounting plate is connected to the other end of the crank, and the cap screwing assembly is mounted on the mounting plate.

As a preferred technical solution of the present invention, the support plates are longitudinally arranged in a group, the lifting assembly includes second guide rails, second sliders and a moving plate, the second guide rails are disposed between each group of the support plates, the second sliders are disposed on each second guide rail, and the moving plate is disposed between the two second sliders.

As a preferable technical solution of the present invention, the capping assembly includes a fourth driving motor, a transmission shaft, and a capping barrel, the fourth driving motor is mounted on the mounting plate, one end of the transmission shaft penetrates through the mounting plate and is connected to an output end of the fourth driving motor, and the capping barrel is disposed at the other end of the transmission shaft.

In another aspect, the present invention provides an antigen expanding culture preservation method, comprising the following steps:

s1, placing a rack with expansion culture test tubes, and forming second constant-temperature cavities between a plurality of expansion culture test tubes and a preservation barrel;

s2, after each preservation barrel is placed in the preservation barrel, a first constant-temperature cavity is formed between the preservation barrel and the preservation barrel, and the upper cover and the preservation barrel are covered by a cover screwing mechanism;

and S3, the cold/hot air is input into the first constant temperature cavity by the cold/hot adjusting equipment so as to surround the cold/hot air on the surface of the storage barrel, and simultaneously enters the second constant temperature cavity through the first side wall which forms a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity and surrounds among the expansion culture test tubes, and the expansion culture test tubes can be kept at constant temperature.

Compared with the prior art, the invention has the beneficial effects that:

(1) After the rack is filled with the expanding culture test tubes, a plurality of expanding culture test tubes and the preservation barrel form a second constant-temperature cavity. After each preservation barrel is arranged in the preservation barrel, a first constant-temperature cavity is formed between the preservation barrel and the preservation barrel, and the upper cover and the preservation barrel are covered by a cover screwing mechanism so as to form a narrow closed space; meanwhile, the cold/hot air is input into the first constant temperature cavity by the cold/hot air adjusting device so that the cold/hot air surrounds the surface of the storage barrel, enters the second constant temperature cavity through the first side wall which is formed into a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity, can flow in the second constant temperature cavity and surrounds among the expanding culture test tubes, and can keep the temperature of the expanding culture test tubes constant.

(2) The screw capping mechanism comprises a back plate, a support plate, a lifting assembly, a driver, a crank, a mounting plate and a screw capping assembly. The screw capping mechanism can enable the upper cover to be opened and closed with the preservation barrel, so that the operation cannot be performed, the labor intensity is reduced, and convenience is provided for preservation of the expanding culture test tube.

The foregoing description is only an overview of the technical solutions of the present invention, and the embodiments of the present invention are described below in order to make the technical means of the present invention more clearly understood and to make the above and other objects, features, and advantages of the present invention more clearly understandable.

Drawings

FIG. 1 is a perspective view of an antigen expanding culture and preservation device disclosed in the present invention;

FIG. 2 is a sectional top view of an antigen expanding culture and preservation apparatus disclosed in the present invention;

FIG. 3 is a schematic structural diagram of a transmission part of the antigen culture and amplification preservation device disclosed by the invention;

FIG. 4 is a schematic view of the storage box of the antigen expanding culture and preservation device disclosed in the present invention;

FIG. 5 is an enlarged view of a portion of FIG. 4 at A;

FIG. 6 is a sectional view of the storage container of the antigen expanding and preserving apparatus of the present invention;

FIG. 7 is a schematic structural diagram of a rack of the antigen expanding culture and preservation apparatus disclosed in the present invention;

FIG. 8 is a schematic view of the injection assembly of an antigen expanding and preserving apparatus disclosed in the present invention;

FIG. 9 is a schematic structural diagram of a screw cap mechanism of the antigen expanding culture preservation device disclosed by the invention;

FIG. 10 is a flow chart of a method for expanding culture and preservation of antigens disclosed by the invention.

Description of the reference numerals: 100. a cabinet; 101. double doors are opened; 102. an operation surface; 110. an accommodating cavity; 111. a rotating shaft; 112. rolling a groove; 120. rotating the plate; 121. connecting holes; 130. a first drive motor; 140. a transmission member; 141. a driving wheel; 142. a drive toothed belt; 150. an injection assembly; 151. a moving member; 1511. a first guide rail; 1512. a first slider; 1513. connecting blocks; 1514. a screw; 1515. a second drive motor; 152. an injection member; 1521. a delivery box; 1522. a delivery needle; 160. a support bar; 161. a ball bearing; 200. a preservation institution; 210. a storage box; 211. an accommodating cavity; 212. an inner groove; 213. a connecting cavity; 220. a preservation cylinder; 221. a first constant temperature cavity; 222. a first protrusion; 230. a preservation barrel; 231. a second constant temperature cavity; 232. a first side wall; 233. an upper cover; 2321. a second protrusion; 240. a rack; 241. a placing hole; 250. a rotating member; 251. a first gear; 252. a second gear; 253. a third drive motor; 300. a cap screwing mechanism; 310. a back plate; 311. a turning groove; 320. a support plate; 330. a lifting assembly; 331. a second guide rail; 332. a second slider; 333. moving the plate; 3331. a through hole; 340. a driver; 350. a crank; 351. a limiting plate; 360. mounting a plate; 370. a screw cap assembly; 371. a fourth drive motor; 372. a drive shaft; 373. and (5) screwing the cover cylinder.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

Example one

Referring to the attached drawings 1-9, the invention provides a technical scheme: an antigen expanding culture preserving device comprises a cabinet 100, a preserving mechanism 200 and a screw cover mechanism 300, wherein the preserving mechanism 200 can be positioned in the cabinet 100 and can provide a storing space for the preserving mechanism 200; the preservation mechanism 200 is used for providing a constant-temperature preservation space for the expanding culture test tube, so that the antigen is always in a proper temperature state, and the expanding culture failure is avoided; and the screw capping mechanism 300 can make the upper cover 233 open and close with the preservation barrel 230, so that manual operation is not needed, the labor intensity is reduced, and convenience is provided for preservation of the expanded culture test tube.

In this embodiment, the cabinet 100 has an accommodating chamber 110, the preservation mechanism 200 is disposed in the accommodating chamber 110, the preservation mechanism 200 includes a preservation box 210, the preservation box 210 has an accommodating chamber 211 and an inner groove 212, the accommodating chamber 211 is used for installing a cooling and heating adjustment device, the inner groove 212 has preservation barrels 220 distributed along the circumferential direction, each preservation barrel 220 is provided with a preservation barrel 230, a first constant temperature cavity 221 is formed between the preservation barrel 230 and the preservation barrel 220, each preservation barrel 230 can be provided with a shelf 240, when the shelf 240 is filled with the expanded culture test tubes, a second constant temperature cavity 231 is formed between the expanded culture test tubes and the preservation barrel 230, the second constant temperature cavity 231 is communicated with the first constant temperature cavity 221, each preservation barrel 230 is covered by an upper cover 233, and the upper cover 233 is in threaded connection with the preservation barrel 230; wherein, the preservation barrel 230 has a first side wall 232, the first side wall 232 is a net shape; the screw-capping mechanism 300 is arranged in the circumferential direction of the storage container 210.

Illustratively, after the rack 240 is filled with the expanding culture tubes, a second constant temperature cavity 231 is formed between the expanding culture tubes and the preservation barrel 230. After each preservation barrel 230 is placed in the preservation barrel 220, a first constant temperature cavity 221 is formed between the preservation barrel 230 and the preservation barrel 220, and the upper cover 233 and the preservation barrel 220 are covered by the cover screwing mechanism 300, so that a narrow closed space (namely, a second constant temperature cavity 231) is formed between the upper cover 233 and the preservation barrel 230; meanwhile, the cold/hot air is input into the first constant temperature cavity 221 by the cold/hot air adjusting device, so that the cold/hot air surrounds the surface of the storage barrel 230, and simultaneously enters the second constant temperature cavity 231 through the first side wall 232 which is formed into a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity 231, the cold/hot air can flow in the second constant temperature cavity 231 and surround between the propagation test tubes, and the propagation test tubes can be kept at a constant temperature.

In an alternative embodiment, the bottom of each storage cylinder 220 is opened with an air inlet and an air outlet, and the cooling and heating adjusting device is communicated with the air inlet and the air outlet through two conduits. On the one hand, the circulation of cold/hot air in the preservation cylinder 220 and the preservation cylinder 230 can be realized; on the other hand, the circulation of the cold/hot air enables the pressure of the preservation canister 220 and the preservation canister 230 to be balanced with the outside.

In an alternative embodiment, the cabinet 100 is provided with a hinged double door 101, and gaps between the double door 101 and the cabinet 100 can be sealed by sealing strips, so that the accommodating cavity 110 can also form a sealed space. Thereby reducing the air flow therein and helping the storage box 210 to maintain a constant temperature.

In an alternative embodiment, cabinet 100 has a work surface thereon. Cabinet 100 not only provides the preservation function, but also may implement the functionality of a testing platform.

In an alternative embodiment, the receiving chamber 110 is provided with a rotating plate 120 rotatable by a rotating shaft 111, and the preservation mechanism 200 and the screw capping mechanism 300 are both provided on the rotating plate 120. The rotating shaft 111 provides a shaft function for the rotating plate 120, two ends of the rotating shaft 111 are respectively rotatably connected with the top and the bottom of the cabinet 100, and the rotating plate 120 can rotate clockwise under the condition that the rotating plate 120 and the rotating shaft 111 are fixed.

For example, after the rotating shaft 111 is driven, the rotating shaft 111 drives the rotating plate 120 to rotate in an arc shape toward the outside of the cabinet 100, so that the storage box 210 can be located outside the cabinet 100, and the staff can inject the substances such as antigens into the expanding culture tube. When the injection is completed, the rotation shaft 111 can drive the rotation plate 120 to return to the initial position again.

In an alternative embodiment, the transmission member 140 may be a transmission between gear sets. In the embodiment of the present invention, the rotating shaft 111 is connected to a transmission member 140, the transmission member 140 includes two rotating wheels and a transmission toothed belt 142, the two rotating wheels are in transmission connection with each other through the transmission toothed belt 142, one rotating wheel is sleeved on the rotating shaft 111, and the other rotating wheel is in transmission connection with the first driving motor 130. The transmission member 140 can drive the rotation shaft 111 to rotate so as to screw in or screw out the rotation plate 120.

For example, when the first driving motor 130 is activated, it drives one of the wheels to rotate, and the two wheels can rotate together by the action of the driving toothed belt 142, so as to drive the rotating shaft 111.

In an alternative embodiment, the receiving cavity 110 is provided with an injection assembly 150 at a side opposite to the inner recess 212, the injection assembly 150 includes a moving part 151 and an injection part 152, and the injection part 152 is connected to the moving part 151. The injection assembly 150 allows for the injection of biological growth material into the expanded culture tube without opening the double door 101 to provide sufficient nutrients for antigen growth.

In an alternative embodiment, the moving member 151 includes a first guide rail 1511, a first sliding block 1512 is disposed on the first guide rail 1511, a connection block 1513 is disposed on the first sliding block 1512, a screw 1514 is connected to the connection block 1513, and the screw 1514 is connected to an output end of the second driving motor 1515. The second driving motor 1515 is driven to move the first block 1512 along the first guide 1511, so that the position of the injection member 152 can be adjusted.

Illustratively, the center of the connection block 1513 has a threaded bore, and the screw 1514 is threadedly coupled to the threaded bore. When the second driving motor 1515 is powered on, it drives the screw 1514 to rotate, so that the connection block 1513 drives the first slide 1512 to move along the first guide rail 1511.

In an alternative embodiment, the injection member 152 includes a delivery box 1521 and a delivery needle 1522, the delivery box 1521 is connected to the connection block 1513, and a plurality of delivery needles 1522 are distributed on the delivery box 1521 in a circular array. Can store partial nutrient solution in the transfer case 1521, when needing to pour into the nutrient solution, can open the valve between transfer needle 1522 and the transfer case 1521, inject the nutrient solution into the culture test tube through transfer needle 1522.

In an alternative embodiment, the rotating plate 120 has a connecting hole 121, a supporting rod 160 is disposed in the connecting hole 121, the supporting rod 160 is fixed to the rotating plate 120, two ends of the supporting rod 160 are provided with balls 161, and the top and the bottom of the accommodating chamber 110 are both provided with rolling grooves 112 for allowing the balls 161 to move in an arc shape. The support rod 160 is used for providing a supporting function for the rotating plate 120 in the rotating process of the rotating plate 120, and ensuring that the rotating plate 120 can be rotated.

Illustratively, the diameter of the roller slot 112 is slightly smaller than the diameter of the ball 161 so that the roller slot 112 just captures a portion of the ball 161 in triplicate. When the rotating plate 120 is rotated, the rolling groove 112 not only limits the ball 161, but also enables the support rod 160 to move in an arc around the rolling groove 112 through the ball 161.

In an alternative embodiment, the inner wall of the preservation barrel 230 is provided with a first protrusion 222, and the preservation barrel 230 is provided with a first groove matched with the first protrusion 222. The first recess and the first protrusion 222 cooperate to prevent the storage container 230 from being rotated during the process of opening and closing the upper lid 233 by the tightening device.

In an alternative embodiment, the supporter 240 has a plurality of supporter holes 241 distributed in a circular array. Should put thing hole 241 and can supply to expand to cultivate the test tube and insert and preserve in bucket 230, put the marginal part in thing hole 241 simultaneously and can provide the supporting role for expanding to cultivate the test tube to make expand to cultivate the test tube and preserve bucket 230 and form second constant temperature cavity 231.

In an alternative embodiment, a rotating member 250 is disposed between the storage tub 230 and the rotating plate 120, the rotating member 250 includes a first gear 251, a second gear 252, and a third driving motor 253, the first gear 251 is fixed on the rotating plate 120, the second gear 252 is engaged with the first gear 251, an output end of the third driving motor 253 is connected with the second gear 252, and the third driving motor 253 is mounted on the storage tub 230. The third driving motor 253 drives the second gear 252 to rotate, and can drive the first gear 251 to drive the preservation barrel 230 to move circumferentially.

Illustratively, during the process of injecting the nutrient solution, the preservation cylinder 220 to be injected is firstly rotated to the screw capping mechanism 300 by the rotating component 250 to be uncapped, and the rotating component 250 rotates the preservation cylinder 230 again to enable the preservation cylinder 220 to be injected to be located at the injecting component 250 to be injected with the nutrient solution; and after completion, the rotating member 250 finally rotates the preservation barrel 230 to screw-cap the preservation barrel at the screw-cap mechanism 300.

In an alternative embodiment, the storage tub 230 is provided with a connection chamber 213, and the rotating plate 120 may be bearing-connected to the connection chamber 213 through a support column. The preservation barrel 230 can be rotated more smoothly.

In an alternative embodiment, the screw capping mechanism 300 includes a back plate 310, supporting plates 320, a lifting assembly 330, a driver 340, a crank 350, a mounting plate 360 and a screw capping assembly 370, wherein a turning groove 311 is formed on the back plate 310, the linear array of supporting plates 320 is formed on the back plate 310, the lifting assembly 330 is disposed between the supporting plates 320, a telescopic end of the driver 340 is connected to the lifting assembly 330, one end of the crank 350 is movably inserted into the turning groove 311, the mounting plate 360 is connected to the other end of the crank 350, and the screw capping assembly 370 is mounted on the mounting plate 360. The crank 350 is in a zigzag shape. In an embodiment of the present invention, the driver 340 is one of an air cylinder, a hydraulic cylinder, or an electric push rod. The driver 340 pushes the lifting assembly 330 to make the mounting plate 360 move linearly along the height of the back plate 310, and the crank 350 drives the mounting plate 360 to be parallel or perpendicular to the storage box 210 along the path of the direction-changing groove 311. Thereby providing a rotating space for the storage bucket 230 when the mounting plate 360 is moved upward; when the mounting plate 360 moves downward, the feeding needle 1522 can be inserted into the culture tube and the nutrient solution can be injected.

Illustratively, the driver 340 pushes the lifting assembly 330 to move the mounting plate 360 upward, and the crank 350 rotates along with the change of the direction-changing slot 311, so that the mounting plate 360 is perpendicular to the storage box 210, and the screw-on cover assembly 370 is parallel to the storage box 210. Similarly, the driver 340 pushes the lifting assembly 330 to drive the mounting plate 360 to move downwards, and the crank 350 rotates along with the change of the direction-changing groove 311, so that the mounting plate 360 is parallel to the storage box 210, and the screw-on cover assembly 370 is perpendicular to the storage box 210. With continued lowering of the mounting plate 360, the delivery needle 1522 may be slowly inserted into the culture tube.

In an alternative embodiment, the moving plate 333 is formed with a through hole 3331 through which the crank 350 passes. The crank 350 is free to spin within the through-hole 3331 such that the crank 350 can rotate the mounting plate 360.

In an alternative embodiment, the end of the crank 350 near the direction-changing groove 311 clamps the back plate 310 through two limiting plates 351, and a slight gap is formed between the limiting plates 351 and the back plate 310. When the crank 350 is moved, the limiting plate 351 limits the crank 350, and prevents the crank 350 from separating from the direction-changing groove 311.

In an alternative embodiment, the supporting plates 320 are arranged in a group, the lifting assembly 330 includes second guide rails 331, second sliders 332 and a moving plate 333, the second guide rails 331 are disposed between the supporting plates 320, the second sliders 332 are disposed on each of the second guide rails 331, and the moving plate 333 is disposed between the two second sliders 332. When the moving plate 333 is driven, the two second sliders 332 can move longitudinally along the length direction of the second rail 331.

In an alternative embodiment, the screw capping assembly 370 includes a fourth driving motor 371, a driving shaft 372 and a screw capping cylinder 373, the fourth driving motor 371 is mounted on the mounting plate 360, one end of the driving shaft 372 penetrates the mounting plate 360 and is connected to an output end of the fourth driving motor 371, and the screw capping cylinder 373 is provided at the other end of the driving shaft 372. The fourth driving motor 371 can drive the transmission shaft 372 to rotate after being started, so that the screwing cover cylinder 373 can be transmitted by the transmission shaft 372, and the screwing cover cylinder 373 can be opened and closed on the upper cover 233.

In an alternative embodiment, the upper cover 233 has a second protrusion 2321 opposite to the first protrusion, and the screw-on cylinder 373 has a second groove (not shown) corresponding to the second protrusion 2321. Therefore, during the process of applying force to the upper cover 233 by screwing the cover cylinder 373, the second groove and the second protrusion 2321 work together to facilitate the opening and closing of the upper cover 233.

Example two

Referring to fig. 10, another antigen expanding culture preservation method provided in the embodiment of the present invention includes the following steps:

s1, after placing the rack 240 with the expanding culture test tubes, a second constant temperature cavity 231 is formed between the plurality of expanding culture test tubes and the preservation barrel 230;

s2, after each preservation barrel 230 is placed in the preservation barrel 220, a first constant-temperature cavity 221 is formed between the preservation barrel 230 and the preservation barrel 220, and the upper cover 233 and the preservation barrel 220 are covered by the cover screwing mechanism 300;

and S3, the cold and hot adjusting device inputs cold/hot air to the first constant temperature cavity 221 so that the cold/hot air surrounds the surface of the storage barrel 230 and enters the second constant temperature cavity 231 through the first side wall 232 which is formed into a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity 231 and surrounds among the expanding culture test tubes, and the expanding culture test tubes can be kept at a constant temperature.

It should be noted that the model specifications of the first driving motor 130, the second driving motor 1515, the third driving motor 253, the fourth driving motor 371, and the driver 340 need to be determined by type selection according to the actual specification of the device, and the specific type selection calculation method adopts the prior art in the field, and therefore, detailed description is omitted.

The power supply of the first, second, third, fourth and driver 130, 1515, 253, 371 and 340 and their principles will be apparent to those skilled in the art and will not be described in detail herein.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. An antigen expanding culture preserving device, which is characterized by comprising:

a cabinet having an accommodation chamber;

the preservation mechanism is arranged in the containing cavity and comprises a preservation box, the preservation box is provided with a containing cavity and an inner groove, the containing cavity is used for installing cold and heat adjusting equipment, preservation cylinders distributed along the circumferential direction are arranged in the inner groove, a preservation barrel is arranged in each preservation cylinder, a first constant-temperature cavity is formed between each preservation barrel and each preservation cylinder, a storage rack can be placed in each preservation barrel, after the storage rack is filled with the expansion culture test tubes, a second constant-temperature cavity is formed between each expansion culture test tube and each preservation cylinder and is communicated with the first constant-temperature cavity, and each preservation cylinder is covered by an upper cover;

wherein the preservation barrel is provided with a first side wall which is net-shaped;

the screwing-cover mechanism is arranged along the circumferential direction of the storage box.

2. The antigen expanding culture preserving device according to claim 1, wherein the accommodating cavity is provided with a rotating plate which can rotate through a rotating shaft, and the preserving mechanism and the screwing mechanism are both arranged on the rotating plate.

3. The antigen expanding culture and preservation device according to claim 2, wherein the rotating shaft is connected with a transmission component, the transmission component comprises two rotating wheels and a transmission toothed belt, the two rotating wheels are in transmission connection through the transmission toothed belt, one rotating wheel is sleeved on the rotating shaft, and the other rotating wheel is in transmission connection with the first driving motor.

4. The antigen expanding culture preservation device according to claim 1, wherein an injection assembly is arranged on a face of the accommodating cavity opposite to the inner groove, the injection assembly comprises a moving part and the injection part, and the injection part is connected with the moving part.

5. The antigen expanding culture and preservation device according to claim 4, wherein the moving member comprises a first guide rail, the first guide rail is provided with a first sliding block, the first sliding block is provided with a connecting block, the connecting block is connected with a screw rod, and the screw rod is connected with an output end of the second driving motor.

6. The antigen expanding culture and preservation device according to claim 5, wherein the injection component comprises a delivery box and the delivery needles, the delivery box is connected with the connecting block, and a plurality of delivery needles are distributed on the delivery box in a circular array.

7. The antigen expanding culture preservation device according to claim 1, wherein the screw cap mechanism comprises a back plate, support plates, lifting components, a driver, a crank, a mounting plate and a screw cap component, the back plate is provided with turning grooves, the back plate is provided with the support plates in a linear array, the lifting components are arranged between the support plates, the telescopic end of the driver is connected with the lifting components, one end of the crank is movably connected in the turning grooves in a penetrating manner, the mounting plate is connected to the other end of the crank, and the screw cap component is mounted on the mounting plate.

8. The antigen expanding culture and preservation device according to claim 7, wherein the support plates are arranged in a group, the lifting assembly comprises second guide rails, second sliding blocks and a moving plate, the second guide rails are arranged between the support plates in each group, the second sliding blocks are arranged on the second guide rails, and the moving plate is arranged between the second sliding blocks.

9. The antigen expanding culture preserving device according to claim 8, wherein the screw cap assembly comprises a fourth driving motor, a transmission shaft and a screw cap cylinder, the fourth driving motor is mounted on the mounting plate, one end of the transmission shaft penetrates through the mounting plate and is connected with an output end of the fourth driving motor, and the screw cap cylinder is arranged at the other end of the transmission shaft.

10. An antigen expanding culture preserving method applied to an antigen expanding culture preserving device of claims 1-9, which is characterized by comprising the following steps:

s1, placing a plurality of expanding culture test tubes on a storage rack, and forming second constant-temperature cavities between the plurality of expanding culture test tubes and a storage barrel;

s2, after each preservation barrel is placed in the preservation barrel, a first constant-temperature cavity is formed between the preservation barrel and the preservation barrel, and the upper cover and the preservation barrel are covered by a cover screwing mechanism;

and S3, the cold/hot air is input into the first constant temperature cavity by the cold/hot adjusting equipment so as to surround the cold/hot air on the surface of the storage barrel, and simultaneously enters the second constant temperature cavity through the first side wall which forms a net shape, so that part of the cold/hot air is concentrated in the second constant temperature cavity and surrounds among the expansion culture test tubes, and the expansion culture test tubes can be kept at constant temperature.

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