CN112680355B - Extensible structure and universal cancer organoid in-vitro culture device - Google Patents
Extensible structure and universal cancer organoid in-vitro culture device Download PDFInfo
- Publication number
- CN112680355B CN112680355B CN202110051484.2A CN202110051484A CN112680355B CN 112680355 B CN112680355 B CN 112680355B CN 202110051484 A CN202110051484 A CN 202110051484A CN 112680355 B CN112680355 B CN 112680355B
- Authority
- CN
- China
- Prior art keywords
- point plate
- sliding
- plate
- piece
- mounting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention discloses medical equipment, in particular to an expandable structure and universal cancer organ in vitro culture device, wherein a guide rail is provided with an installation part in a sliding manner, one side of the installation part is rotatably provided with a residual gear, and rack plates matched with the residual gear are horizontally arranged on the side walls of a first point plate and a second point plate; the sample applicator also comprises a power component arranged on the mounting component, a sample application component movably arranged on the mounting component and connected with the power component, and a sample supply component; when the residual gear rotates, the toothed part on the residual gear is discontinuously combined with the rack plate on the second point plate to drive the second point plate to perform horizontal movement, the elastic structure drives the first point plate to be lifted to be flush with the second point plate, and the mounting part drives the residual gear to perform discontinuous horizontal movement under the action of the guide rail; the worm drives the worm wheel and the sample application component to move synchronously, the sample application component drops the cancer cell matrigel into the dot pits arranged on the dot plate in an array manner, and the cancer cell matrigel is evenly dropped on the second dot plate and the first dot plate for culture.
Description
Technical Field
The invention relates to a medical apparatus, in particular to an expandable structure and universal cancer organoid in vitro culture device.
Background
In recent years, the in vitro cell culture technology has been developed rapidly, and now the cell culture is not only the traditional plane culture mode, but also the cells can form a three-dimensional structure in vitro through various modes.
The current organ culture technology mainly uses stem cells, induces the stem cells to differentiate into different types of cells in an organ by simulating the environment of human development, and forms the basic structure of the organ. Shearing and digesting the cancer tissue sample, then carrying out heavy suspension treatment on the cancer cells obtained after digestion treatment by using precooled matrigel, carrying out spot plate treatment on the cancer cell suspension, and carrying out culture treatment on the cancer cells subjected to spot plate treatment to finally obtain the cancer organoid.
The point board is handled and is to be about to cancer cell matrix glue and be the matrix dropwise add to the TLC board on, then cultivates it, and current point board means of cultivateing is mostly through manual operation, because the cultivation success rate is not one hundred per cent, consequently need cultivate a plurality ofly, needs great TLC board this moment, requires highly to the stroke of equipment through the machine point sample, consequently can only cultivate by a small amount, is unfavorable for a plurality of organs and cultivates simultaneously.
Disclosure of Invention
Based on the above-mentioned shortcomings in the prior art, the present invention provides an expandable structure and universal cancer organoid in vitro culture device.
The invention overcomes the technical problems by adopting the following technical scheme, and specifically comprises the following steps:
an expandable structure comprises a base, a first point plate and a second point plate, wherein the first point plate and the second point plate are horizontally stacked on the base, a plurality of loop bars which are in sliding fit with the first point plate are vertically fixed on one side of the base, and the second point plate is connected with the first point plate through an elastic sliding assembly;
the rack plate assembly comprises a base, a first point plate and a second point plate, wherein two horizontal guide rails are respectively arranged on two sides of the base, an installation part is arranged on each guide rail in a sliding manner, a residual gear used for driving the installation part to horizontally move relative to the first point plate and/or the second point plate is rotatably arranged on one side of the installation part, and rack plates matched with the residual gears are horizontally arranged on the side walls of the first point plate and the second point plate;
the elastic sliding assembly comprises a sliding structure arranged on the first point plate and an elastic structure connected with the sliding structure and the second point plate, a horizontal caulking groove is formed in the side wall of the back face of the second point plate, and a convex block which is in sliding embedded with the caulking groove is arranged on the inner side of the back face of the lower portion of the mounting piece.
As a further scheme of the invention: the elastic sliding assembly comprises a sliding structure arranged on the first point plate and an elastic structure connecting the sliding structure and the second point plate; the sliding structure comprises a sliding groove horizontally arranged on the side wall of the first point plate and a sliding block arranged on the sliding groove in a sliding manner;
the elastic structure comprises a sleeve seat, an expansion piece and a pressure spring, wherein one end of the sleeve seat is hinged with the second point plate, the expansion piece penetrates through the other end of the sleeve seat and is coaxially and slidably connected with the sleeve seat, and the pressure spring is used for elastically connecting the sleeve seat and the expansion piece;
one end of the telescopic piece, which is far away from the sleeve seat, is hinged with the sliding block.
A universal cancer organoid in vitro culture device, which comprises an expandable structure, a power component arranged on the mounting component, a sample application component movably arranged on the mounting component and connected with the power component, and a sample supply component connected with the power component and the sample application component;
the sample feeding assembly is connected with the residual gear, the sample application assembly reciprocates along a direction which is vertical to the second sample application plate in space, and the power assembly comprises power equipment fixed on the upper part of one side of the mounting piece, a worm which is rotatably arranged on the mounting piece and connected with the output end of the power equipment, and a worm wheel which is rotatably arranged on the mounting piece and meshed with the worm;
the sample application component is connected with the worm, and the sample supply component is connected with the worm wheel.
As a further scheme of the invention: the sample application assembly comprises a movable structure arranged on the mounting part and a pushing structure used for connecting the movable structure and the worm;
the movable structure comprises a plurality of sliding rods which are fixed on the mounting part in equal height and a movable part which is arranged on the sliding rods in a sliding mode, the pushing structure is connected with the movable part, and a dropping head communicated with the sample supply assembly is arranged below the movable part.
As a still further scheme of the invention: the pushing structure comprises a rotating piece fixed at the lower part of the worm, a push rod vertically fixed at the edge of the lower surface of the rotating piece, and two baffle plates fixed on the moving piece in parallel;
and a concave part is formed between the two baffles, and the push rod is clamped in the concave part in a sliding manner.
As a still further scheme of the invention: the sample supply assembly comprises a drawing tube fixed on the mounting part, a conduit connecting the upper part of the drawing tube and the drip emitter, and a sealing element hermetically and movably arranged in the drawing tube;
the sealing element is connected with the worm wheel through a slider-crank structure, a one-way valve is installed at the joint of the guide pipe and the pumping cylinder, and a sampling pipe is installed at the top of the pumping cylinder through another one-way valve.
As a still further scheme of the invention: the crank slider structure comprises a middle rotating wheel rotatably mounted on the mounting piece, a first transmission piece connected with the middle rotating wheel and the worm wheel, and a connecting rod connected with the middle rotating wheel and the sealing piece;
one end of the connecting rod is pivoted with the eccentric part of the middle rotating wheel, the other end of the connecting rod is pivoted with the sealing element, and a through groove for the connecting rod to pass through is formed in one side, close to the middle rotating wheel, of the drawing barrel.
As a still further scheme of the invention: the transfer wheel is connected with the residual gear through a second transmission piece, the upper portion of the sleeve rod is provided with threads, and the threads are connected with a limiting nut.
After adopting the structure, compared with the prior art, the invention has the following advantages: when the residual gear rotates, the toothed part on the residual gear is discontinuously combined with the rack plate on the second point plate to drive the second point plate to discontinuously and horizontally move under the guiding action of the caulking groove and the bump, when the second point plate moves to the stroke end of the second point plate, the first point plate below the second point plate is driven to be lifted to be flush with the second point plate through the elastic structure, the residual gear starts to be combined with the rack plate on the first point plate at the moment, and then the mounting part drives the residual gear to discontinuously and horizontally move under the action of the guide rail, so that the horizontal expansion function is realized;
the worm is driven to rotate when the power equipment works, the worm drives the worm wheel and the sample application component to synchronously move, the sample application component drips cancer cell matrix glue into the pits arranged on the spot plate in an array manner, and the second spot plate or the mounting piece which is in discontinuous activity is matched to uniformly drip cancer cell matrix glue on the second spot plate and the first spot plate for culture.
Drawings
FIG. 1 is a schematic structural diagram of a universal cancer organoid in vitro culture apparatus.
FIG. 2 is a schematic structural diagram of a baffle plate and a movable member in the universal cancer organoid in-vitro culture device.
FIG. 3 is a schematic diagram of the structure of the mounting member, the sliding rod and the bump in the universal cancer organoid in vitro culture device.
Fig. 4 is a partially enlarged schematic view of a portion a of fig. 1.
In the figure: 1-a base; 2-point board number one; 3-point board II; 4-a mounting member; 5-a guide rail; 6-a power plant; 7-a worm; 8-a rotating member; 9-a push rod; 10-a baffle plate; 11-a movable member; 12-a slide bar; 13-a worm gear; 14-a first transmission member; 15-a middle rotating wheel; 16-a connecting rod; 17-a seal; 18-a pump cylinder; 19-a catheter; 20-dropping of a dripper; 21-a second transmission member; 22-a spur gear; 23-a rack plate; 24-a socket; 25-a telescoping member; 26-a pressure spring; 27-a slide block; 28-a chute; 29-a loop bar; 30-bumps.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
In addition, an element of the present invention may be said to be "fixed" or "disposed" to another element, either directly on the other element or with intervening elements present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Referring to fig. 1 to 4, in the embodiment of the present invention, an expandable structure includes a base 1, a first point plate 2 and a second point plate 3 horizontally stacked on the base 1, wherein a plurality of loop bars 29 slidably sleeved with the first point plate 2 are vertically fixed on one side of the base 1, and the second point plate 3 is connected with the first point plate 2 through an elastic sliding assembly;
specifically, two horizontal guide rails 5 are respectively installed on two sides of the base 1, an installation part 4 is slidably arranged on each guide rail 5, a residual gear 22 for driving the installation part 4 to horizontally move relative to the first point plate 2 and/or the second point plate 3 is rotatably installed on one side of the installation part 4, and rack plates 23 matched with the residual gear 22 are horizontally installed on the side walls of the first point plate 2 and the second point plate 3;
in detail, the elastic sliding assembly comprises a sliding structure arranged on the first point plate 2 and an elastic structure connecting the sliding structure and the second point plate 3, a horizontal caulking groove is formed in the side wall of the back surface of the second point plate 3, and a convex block 30 which is in sliding embedding with the caulking groove is arranged on the inner side of the back surface of the lower part of the mounting part 4;
as an explanation of this embodiment, the toothed portion of the edge portion of the residual gear 22 is smooth, the toothed portion of the residual gear is adapted to the rack plate 23, when the residual gear 22 rotates, the toothed portion on the residual gear is intermittently combined with the rack plate 23 on the second point plate 3, the second point plate 3 is driven to intermittently and horizontally move under the guiding action of the caulking groove and the projection 30, when the second point plate 3 moves to the end of the stroke, the first point plate 2 below is driven by the elastic structure to be lifted to be flush with the second point plate 3, at this time, the residual gear 22 starts to be combined with the rack plate 23 on the first point plate 2, and then the mounting member 4 drives the residual gear 22 to intermittently and horizontally move under the action of the guide rail 5, so as to implement the horizontal expansion function.
In order to ensure that the mounting part 4 keeps relatively static with the guide rail 5 when the second point board 3 moves horizontally, a damping sleeve is arranged at the lower part of the mounting part 4 in a penetrating way, the mounting part 4 is connected with the guide rail 5 in a sliding way through the damping sleeve, and the friction force between the guide rail 5 and the mounting part 4 is larger due to the action of the damping sleeve, so that when the toothed part on the residual gear 22 is combined with the rack board 23 on the second point board 3, the mounting part 4 keeps relatively static with the guide rail 5, and the second point board 3 moves horizontally.
In addition, in this embodiment, a plurality of wells for accommodating cancer cell matrigel are dug in an array on the upper surfaces of the first spot plate 2 and the second spot plate 3, and the first spot plate 2 and the second spot plate 3 are TLC plates for culturing organ tissues, and may be silica gel plates.
In one embodiment of the invention, the elastic sliding assembly comprises a sliding structure arranged on the point plate No. 2 and an elastic structure connecting the sliding structure and the point plate No. two 3; the sliding structure comprises a sliding groove 28 horizontally arranged on the side wall of the first point plate 2 and a sliding block 27 arranged on the sliding groove 28 in a sliding manner;
the elastic structure comprises a sleeve seat 24, one end of which is hinged with the second point plate 3, an expansion piece 25, which penetrates through the other end of the sleeve seat 24 and is coaxially and slidably connected with the sleeve seat, and a pressure spring 26, which is used for elastically connecting the sleeve seat 24 and the expansion piece 25;
the end of the telescopic element 25 remote from the sleeve seat 24 is hinged with the sliding block 27.
As an illustration of the embodiment, when the second point plate 3 moves horizontally, the sliding block 27 slides along the sliding groove 28, since the first point plate 2 is restrained by the loop bar 29, and the second point plate 3 is restrained by the projection 30 and the caulking groove, both of them are always kept horizontal, when the sliding block 27 slides to the end point of the sliding groove 28, the toothed part on the residual gear 22 continues to drive the second point plate 3 to move, at this time, the expansion piece 25 extends out of the loop base 24, the compression spring 26 is compressed, and at the moment of dislocation between the second point plate 3 and the first point plate 2, the first point plate 2 is driven by the elasticity of the compression spring 26 to lift to be flush with the second point plate 3.
In an embodiment of the present invention, there is also provided a universal cancer organoid in vitro culture apparatus, comprising the expandable structure of the above embodiment, further comprising a power assembly mounted on the mounting member 4, a sample application assembly movably disposed on the mounting member 4 and connected to the power assembly, and a sample supply assembly connected to the power assembly and the sample application assembly;
the sample feeding assembly is connected with the residual gear 22, the sample application assembly reciprocates along the direction which is vertical to the second sample application plate 3 in space, and the power assembly comprises a power device 6 fixed on the upper part of one side of the mounting piece 4, a worm 7 which is rotatably arranged on the mounting piece 4 and is connected with the output end of the power device 6, and a worm wheel 13 which is rotatably arranged on the mounting piece 4 and is meshed with the worm 7;
the sample application component is connected with the worm 7, and the sample supply component is connected with the worm wheel 13;
as the explanation of the embodiment, when the power device 6 works, the worm 7 is driven to rotate, the rotating worm 7 drives the worm wheel 13 and the sample application component to synchronously move, the sample application component drops the cancer cell matrix glue into the dot pits arranged in the dot plate in an array manner, and the cancer cell matrix glue is evenly dropped on the second dot plate 3 and the first dot plate 2 by matching with the discontinuously movable second dot plate 3 or the mounting piece 4 for culture.
For ease of understanding, the power plant 6 includes, but is not limited to, conventional servomotors, hydraulic motors, pneumatic motors, and the like.
In one embodiment of the invention the print module comprises a movable structure mounted on the mounting 4 and a pushing structure for connecting the movable structure to the worm 7;
the movable structure comprises a plurality of sliding rods 12 which are fixed on the mounting part 4 at equal heights and a movable part 11 which is arranged on the sliding rods 12 in a sliding manner, the pushing structure is connected with the movable part 11, and a dropping head 20 communicated with the sample supply component is arranged below the movable part 11;
as the description of the embodiment, when the worm 7 rotates, the moving part 11 is driven by the pushing structure to reciprocate along the sliding rod 12, and further the dropping head 20 is driven to follow the reciprocating motion, the cancer cell matrix glue is dropped into the pits on the dropping plate, and the cancer cell matrix glue is dropped on the second dropping plate 3 and the first dropping plate 2 in an array manner by matching with the continuously moving second dropping plate 3 or the mounting part 4.
In a further embodiment of the invention, the pushing structure comprises a rotating member 8 fixed at the lower part of the worm 7, a push rod 9 vertically fixed at the edge of the lower surface of the rotating member 8, and two baffle plates 10 fixed in parallel on the movable member 11;
wherein, a concave part is formed between the two baffles 10, and the push rod 9 is clamped in the concave part in a sliding way.
As the worm 7 rotates, the rotating member 8 is driven to rotate, the rotating member 8 drives the push rod 9 to move circularly, and the circularly moving push rod 9 drives the concave portion and the two baffle plates 10 and the movable member 11 to reciprocate along the sliding rod 12.
In yet another embodiment of the present invention, the sample feeding assembly comprises a drawing tube 18 fixed on the mounting member 4, a conduit 19 connecting the upper part of the drawing tube 18 and the drip emitter 20, and a sealing member 17 disposed in the drawing tube 18 in a sealing and movable manner;
the sealing element 17 is connected with the worm wheel 13 through a crank slider structure, a one-way valve is arranged at the joint of the guide pipe 19 and the pumping cylinder 18, and a sampling pipe is arranged at the top of the pumping cylinder 18 through another one-way valve;
as the description of the embodiment, the sampling tube is communicated with the prepared cancer cell matrix gel heavy suspension, the worm wheel 13 is driven to rotate when the worm 7 rotates, the worm wheel 13 drives the sealing member 17 to reciprocate through the crank-slider structure, and therefore, the cancer cell matrix gel is intermittently supplied to the dropping head 20 through the pumping tube 18 and the guide tube 19.
In a further embodiment of the invention, the slider-crank arrangement comprises a central wheel 15 rotatably mounted on the mounting member 4, a first transmission member 14 connecting the central wheel 15 with the worm wheel 13, and a connecting rod 16 connecting the central wheel 15 with the sealing member 17;
one end of the connecting rod 16 is pivoted with the eccentric position of the middle rotating wheel 15, the other end of the connecting rod is pivoted with the sealing element 17, and a through groove for the connecting rod 16 to pass through is formed in one side, close to the middle rotating wheel 15, of the drawing cylinder 18;
as the embodiment is explained, the rotating wheel 15 is driven to rotate by the rotating worm wheel 13 through the first transmission piece 14, the rotating wheel 15 drives the connecting rod 16 to drive the sealing piece 17 to move back and forth along the inner wall of the pumping cylinder 18, and finally, two one-way valves are matched to realize that the pumping cylinder 18 supplies cancer cell matrigel samples into the guide pipe 19 discontinuously.
In another embodiment of the present invention, the middle rotating wheel 15 is connected to the residual gear 22 through a second transmission member 21, the upper portion of the sleeve rod 29 is provided with a thread, and a limit nut is connected to the thread;
when the residual gear 22 drives the rack plate 23 on the second point plate 3 to move to the stroke end, the first point plate 2 is lifted up along the loop bar 29 under the action of the elastic sliding assembly, the lifting height of the first point plate is limited by the limit nut in the lifting process, the first point plate is kept as high as the second point plate 3, when the first point plate 2 and the second point plate 3 are level, the cancer cell matrix glue is uniformly dripped into the first point plate 2 through the horizontal movement of the mounting part 4, and after the first point plate 2 and the second point plate 3 are fully dripped with the cancer cell matrix glue, the lower limit nut can be rotated to take the first point plate 2 and the second point plate out for culture.
The foregoing is merely illustrative of the preferred embodiments of the present invention and is not to be construed as limiting the claims. The present invention is not limited to the above embodiments, and the specific structure thereof is allowed to vary. But all changes which come within the scope of the invention are intended to be embraced therein.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Claims (1)
1. A universal cancer organoid in vitro culture device comprises an expandable structure, wherein the expandable structure comprises a base (1), a first point plate (2) and a second point plate (3) which are horizontally stacked on the base (1), and is characterized in that a plurality of loop bars (29) which are slidably sleeved with the first point plate (2) are vertically fixed on one side of the base (1), and the second point plate (3) is connected with the first point plate (2) through an elastic sliding assembly;
the two sides of the base (1) are respectively provided with a horizontal guide rail (5), the guide rails (5) are provided with mounting pieces (4) in a sliding mode, one side of each mounting piece (4) is rotatably provided with a residual gear (22) used for driving the mounting pieces (4) to horizontally move relative to the first point plate (2) and/or the second point plate (3), and rack plates (23) matched with the residual gears (22) are horizontally arranged on the side walls of the first point plate (2) and the second point plate (3);
the elastic sliding assembly comprises a sliding structure arranged on the first point plate (2) and an elastic structure connecting the sliding structure and the second point plate (3), a horizontal caulking groove is formed in the side wall of the back face of the second point plate (3), and a convex block (30) which is in sliding embedded with the caulking groove is arranged on the inner side of the back face of the lower portion of the mounting piece (4);
the elastic sliding assembly comprises a sliding structure arranged on the first point plate (2) and an elastic structure connecting the sliding structure and the second point plate (3); the sliding structure comprises a sliding groove (28) which is horizontally arranged on the side wall of the first point plate (2) and a sliding block (27) which is arranged on the sliding groove (28) in a sliding manner;
the elastic structure comprises a sleeve seat (24) with one end hinged with the second point plate (3), an expansion piece (25) which penetrates through the other end of the sleeve seat (24) and is coaxially and slidably connected with the sleeve seat, and a pressure spring (26) which is used for elastically connecting the sleeve seat (24) and the expansion piece (25);
one end of the telescopic piece (25) far away from the sleeve seat (24) is hinged with the sliding block (27);
the universal cancer organoid in-vitro culture device also comprises a power component arranged on the mounting member (4), a sample application component movably arranged on the mounting member (4) and connected with the power component, and a sample supply component connected with the power component and the sample application component;
the sample feeding assembly is connected with the residual gear (22), the sample application assembly reciprocates along the direction which is perpendicular to the second sample application plate (3) in space, and the power assembly comprises a power device (6) fixed on the upper part of one side of the mounting piece (4), a worm (7) which is rotatably arranged on the mounting piece (4) and is connected with the output end of the power device (6), and a worm wheel (13) which is rotatably arranged on the mounting piece (4) and is meshed with the worm (7);
the sample application component is connected with the worm (7), and the sample supply component is connected with the worm wheel (13);
the spotting assembly comprises a mobile structure mounted on the mounting (4) and a pushing structure for connecting the mobile structure with the worm (7);
the movable structure comprises a plurality of sliding rods (12) which are fixed on the mounting piece (4) in equal height and a movable piece (11) which is arranged on the sliding rods (12) in a sliding manner, the pushing structure is connected with the movable piece (11), and a dropping head (20) communicated with the sample supply assembly is arranged below the movable piece (11);
the pushing structure comprises a rotating piece (8) fixed at the lower part of the worm (7), a push rod (9) vertically fixed at the edge of the lower surface of the rotating piece (8), and two baffle plates (10) fixed on the moving piece (11) in parallel;
a concave part is formed between the two baffle plates (10), and the push rod (9) is slidably clamped in the concave part;
the sample supply assembly comprises a drawing tube (18) fixed on the mounting part (4), a conduit (19) connecting the upper part of the drawing tube (18) and the drip head (20), and a sealing part (17) which is arranged in the drawing tube (18) in a sealing and movable manner;
the sealing element (17) is connected with the worm wheel (13) through a crank sliding block structure, a one-way valve is installed at the joint of the guide pipe (19) and the pumping cylinder (18), and a sampling pipe is installed at the top of the pumping cylinder (18) through another one-way valve;
the crank slider structure comprises a middle rotating wheel (15) rotatably mounted on the mounting part (4), a first transmission part (14) connecting the middle rotating wheel (15) and the worm wheel (13), and a connecting rod (16) connecting the middle rotating wheel (15) and the sealing part (17);
one end of the connecting rod (16) is pivoted with the eccentric part of the middle rotating wheel (15), the other end of the connecting rod is pivoted with the sealing element (17), and a through groove for the connecting rod (16) to pass through is formed in one side, close to the middle rotating wheel (15), of the drawing barrel (18);
the middle rotating wheel (15) is connected with the residual gear (22) through a second transmission piece (21), the upper portion of the sleeve rod (29) is provided with threads, and the threads are connected with limit nuts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110051484.2A CN112680355B (en) | 2021-01-14 | 2021-01-14 | Extensible structure and universal cancer organoid in-vitro culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110051484.2A CN112680355B (en) | 2021-01-14 | 2021-01-14 | Extensible structure and universal cancer organoid in-vitro culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112680355A CN112680355A (en) | 2021-04-20 |
| CN112680355B true CN112680355B (en) | 2022-04-15 |
Family
ID=75458160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110051484.2A Active CN112680355B (en) | 2021-01-14 | 2021-01-14 | Extensible structure and universal cancer organoid in-vitro culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112680355B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206261833U (en) * | 2016-08-30 | 2017-06-20 | 无锡兰桂医疗股份有限公司 | The moving guide rail group of automatic dispensation apparatus |
| CN110656086A (en) * | 2019-10-11 | 2020-01-07 | 纳肽得(青岛)生物医药有限公司 | In vitro culture method of cancer organoid |
| CN111607495A (en) * | 2020-06-04 | 2020-09-01 | 奥格诺德生物科技(北京)有限公司 | Preparation method of tumor organoid, device and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2018169007A1 (en) * | 2017-03-16 | 2020-02-13 | 株式会社Ｌｓｉメディエンス | Three-dimensional culture of primary cancer cells using tumor tissue |
-
2021
- 2021-01-14 CN CN202110051484.2A patent/CN112680355B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206261833U (en) * | 2016-08-30 | 2017-06-20 | 无锡兰桂医疗股份有限公司 | The moving guide rail group of automatic dispensation apparatus |
| CN110656086A (en) * | 2019-10-11 | 2020-01-07 | 纳肽得(青岛)生物医药有限公司 | In vitro culture method of cancer organoid |
| CN111607495A (en) * | 2020-06-04 | 2020-09-01 | 奥格诺德生物科技(北京)有限公司 | Preparation method of tumor organoid, device and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112680355A (en) | 2021-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20080026465A1 (en) | Cell Culturing Method Using Biomechanical Stimulation Loading And System Therefor | |
| CN103756898A (en) | Three-dimensional stress cell culture device capable of applying dynamic load | |
| CN112680355B (en) | Extensible structure and universal cancer organoid in-vitro culture device | |
| CN113621478A (en) | Nutrient solution timed and equivalent adding equipment suitable for microorganism breeding culture | |
| CN111591940A (en) | Biological cell culture is with automatic device of uncapping | |
| CN112625902A (en) | Bioreactor and have its biological reaction system | |
| CN109009981A (en) | A kind of automatic pressing machine of cardiac resuccitation | |
| CN208374686U (en) | A kind of kit automatic assembly equipment | |
| CN114889919B (en) | Biological bacterial save set | |
| CN115414701B (en) | Biomedical-based biological tissue extraction device and method | |
| CN210367728U (en) | Cell bio-genetic engineering is with bio-pharmaceuticals stem cell reaction equipment | |
| CN213245881U (en) | Efficient and fertilizer-saving plant cultivation vessel | |
| CN212993295U (en) | Culture rack for seedling culture | |
| CN209568108U (en) | Biological culture reaction unit | |
| CN209234508U (en) | A kind of easy-to-dismount mushroom plantation frame | |
| CN208919108U (en) | Ball assembly tooling | |
| CN220389309U (en) | Novel semi-automatic press mounting equipment | |
| CN208308875U (en) | A kind of cell culture apparatus for simulating massage effect | |
| DE10349688A1 (en) | Bioreactor, for tissue engineering and ex situ testing, has a carrier with a biomaterial holder to be moved by a linear drive through a medium to replicate systolic and diastolic pressures | |
| CN218680676U (en) | 16-hole fixed inoculation machine | |
| CN107962370B (en) | Plug assembly for medical machinery production | |
| CN203869974U (en) | Dynamic mechanics experimental device of tissue engineering scaffold | |
| CN116183389B (en) | Crop straw compression intelligent detection test bed | |
| CN110810066A (en) | Novel hierarchical green prickly ash plants device | |
| CN220269139U (en) | Adjusting structure for gas cylinder frame |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |