CN1163618C - Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid - Google Patents
Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid Download PDFInfo
- Publication number
- CN1163618C CN1163618C CNB001166999A CN00116699A CN1163618C CN 1163618 C CN1163618 C CN 1163618C CN B001166999 A CNB001166999 A CN B001166999A CN 00116699 A CN00116699 A CN 00116699A CN 1163618 C CN1163618 C CN 1163618C
- Authority
- CN
- China
- Prior art keywords
- fermentation
- weight
- waste liquid
- carotin
- beta
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Abstract
The present invention relates to a new technology for preparing natural beta-carotin by cyclic utilization of fermented waste liquid. The production steps comprise a slant culture working procedure, a seed culture working procedure and a ferment working procedure. The new technology is characterized in that in the ferment working procedure, mycelium is filtered from the waste liquid after fermentation, and then, the waste liquid is used for replacing clear water for preparing a culture medium for fermentation to produce beta-carotene. The new technology solves the problem of environment protection processing of high concentration organic waste liquid produced in the fermentation production, and a large quantity of investment of three-waste processing equipment is saved. The miscellaneous processing technology and expensive handling cost are eliminated. The present invention is a green technology, and the economical and social benefit is obvious. Experiments prove that the cyclic utilization of fermented waste liquid has no influence to the fermentation level of beta-carotene.
Description
The present invention relates to a kind of technology of preparing natural beta-carotin by fermentation, relate to the novel process that a kind of recycle fermented waste fluid is produced natural beta-carotin or rather.
In the prior art, " biotechnology " 1998 the 8th the 5th phases of volume have been reported a kind of method of utilizing gourmet powder waste water to cultivate Bacillus thuringiensis, the waste water that this method utilizes the monosodium glutamate industrial fermentation to produce is raw material, preparation culture medium culturing Bacillus thuringiensis, determined optimum culture medium prescription, illustrated with gourmet powder waste water and cultivate Bacillus thuringiensis and then produce B
tBiological pesticide is feasible technically.But this technology also rests on laboratory stage at present.
" food and fermentation industries " the 24th the 5th phase of volume has been reported a kind of method of utilizing monosodium glutamate concentrating spent liquor production of protein feed by solid fermentation, test is found, but solid fermentation is produced animal feeding-stuff containing somatic protein after being concentrated into 38% gourmet powder waste water and partial supplementary material being mixed, confirm through the animal rearing test, tropina can replace bean-dregs feed, be suitable for raising livestock and poultry, economic benefit is obvious.
The waste liquid amount of fermentative Production natural beta-carotin generation now generally accounts for 75～80% of fermented liquid total amount, main more residual thalline do not have the nutritive substance that utilized in the waste liquid, wherein total reducing sugar is 0.9～3.6%, reducing sugar is 0.01～0.65%, amino nitrogen also has some micro-manganese metals etc. at 0.20～0.65mg/mL.Fermented waste fluid has features such as concentration height, colourity are big, solid content height, wherein COD
CrValue is up to about 17～220,000 mg/L, and suspended solids (SS) amount is very high, is milk sap, and colourity reaches 40000 times, is the organic waste water of high density, can not directly discharge, and must pass through and could discharge after environmental protection treatment reach national environmental standard.At present the organic waste water resolution recommended of country cuts both ways, though the treatment effect that has is better, complex process invest too greatly, and application economy does not pass a test on the suitability for industrialized production, has restricted the popularization of technology.Therefore, seeking the recycle of fermented waste fluid, realize the zero release of fermented waste fluid, is one of preferred approach that solves organic waste water improvement.
The purpose of this invention is to provide a kind of recycle fermented waste fluid and produce the novel process of natural beta-carotin, the fermented waste fluid of this technology by the recycle producing beta-carotene by fermentation is as the raw material of preparation substratum, reduced the treatment capacity of organic waste water, reduce facility investment, had good economic benefit and social benefit.
The present invention realizes like this.A kind of recycle fermented waste fluid of the present invention is produced the novel process of natural beta-carotin, the production stage of this production technique mainly comprises employing blakeslea trispora (Blakeslea trispora) through slant culture, seed culture, fermentation procedure, and described fermentation procedure is to utilize the mycelial waste liquid preparation of fermentation back filtering substratum to come preparing natural beta-carotin by fermentation.
The waste liquid preparation substratum of described utilization fermentation after-filtration comes preparing natural beta-carotin by fermentation, exactly preceding one time fermentation after-filtration is gone out mycelial waste collection and get up to prepare new fermented liquid, wherein the add-on of carbon source can be done suitable adjustment according to total reducing sugar amount residual in the waste liquid, and other nutritive ingredient can remain unchanged.
The bacterial classification of fermentation adopts produces the best bacterial strain of suitability for industrialized production---the blakeslea trispora (Blakeslea trispora) that natural beta-carotin is generally acknowledged in the world, wherein the slant culture of bacterial classification and seed culture adopt common cultural method, can adopt the cultivation respectively or the mixed culture of positive and negative bacterial strain.Promptly insert fermentation procedure after seed culture finishes and carry out fermentation culture.
Novel process of the present invention, described fermention medium consist of starch 5～8% (weight), dregs of rice cake powder 1.2～2.8% (weight), vegetables oil 5.1～7.0% (weight), potassium primary phosphate 0.04～0.09% (weight), nonionogenic tenside alkylphenol polyoxyethylene 0.05～0.20% (weight), vitamins B
13～10ppm, manganous sulfate 0.01～0.06% (weight), the mycelial waste liquid of back filtering that will ferment again mixes with said components, and insufficient amount of liquid adds clear water to be supplied, and promptly can be used as the substratum of preparing natural beta-carotin by fermentation after stirring.
Novel process of the present invention, described fermentation culture conditions are 26～28 ℃ of culture temperature, incubation time 120～168 hours.
The present invention compared with prior art has the following advantages:
One, the present invention is owing to adopt the recycle fermented waste fluid to produce the novel process of natural beta-carotin, solved the environmental protection treatment problem of the high concentrated organic waste liquid of preparing natural beta-carotin by fermentation generation, be the friendly process of a kind of fermented waste fluid near zero release, economic and social benefit is obvious.
Two, novel process of the present invention has been saved the expensive environmental protection treatment expense of fermented waste fluid and numerous and diverse treatment process, has saved the investment of a large amount of three wastes treatment facilities, has avoided the pollution of fermented waste fluid to environment.
Three, novel process of the present invention confirms through test of many times, and the fermented waste fluid preparation substratum of recycle preparing natural beta-carotin by fermentation is to the not influence of fermentation level of β-Hu Luobusu.During as fermented waste fluid recycle the 6th time, the fermentation level of β-Hu Luobusu is 2.307g/L, and contrast (preparing substratum with clear water) fermentation level is 2.350g/L; During fermented waste fluid recycle the 8th time, the fermentation level of β-Hu Luobusu is 2.436g/L, and the control fermentation level is 2.150g/L.
Further set forth the present invention below by detailed description, but embodiment not a limitation of the present invention to the specific embodiment of the invention.
Embodiment 1:
Fermented waste fluid after fermentation is filtered is for the first time substituted the liquid of clear water as the preparation substratum, toward wherein adding starch 5% (weight), dregs of rice cake powder 1.4% (weight), vegetables oil 6.6% (weight), potassium primary phosphate 0.06% (weight), Triton (Triton) 0.12% (weight), vitamins B
15ppm, manganous sulfate 0.05% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 120 hours fermented liquid, analytical results is as follows: thalline content 18.33%, content beta-carotene 12.097mg/g in the thalline, content beta-carotene 2.218g/L in the fermented liquid.
Comparative Examples 1:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 1.It is as follows to analyze the fermented liquid result: thalline content 16.55%, content beta-carotene 11.430mg/g in the thalline, content beta-carotene 1.892g/L in the fermented liquid.
Embodiment 2:
Except that fermented incubation time is 168 hours, other such as fermentative medium formula and compound method, access bacterial classification amount and culture condition with embodiment 1.It is as follows to analyze the fermented liquid result: thalline content 18.26%, content beta-carotene 12.458mg/g in the thalline, content beta-carotene 2.274g/L in the fermented liquid.
Comparative Examples 2:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 2.It is as follows to analyze the fermented liquid result: thalline content 17.43%, content beta-carotene 11.219mg/g in the thalline, content beta-carotene 1.955g/L in the fermented liquid.
Embodiment 3:
Fermented waste fluid with circulation the 5th substitutes the liquid of clear water as the preparation substratum, toward wherein adding starch 8% (weight), dregs of rice cake powder 1.2% (weight), vegetables oil 7.0% (weight), potassium primary phosphate 0.04% (weight), Triton (Triton) 0.05% (weight), vitamins B
13ppm, manganous sulfate 0.03% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 144 hours fermented liquid, analytical results is as follows: thalline content 16.84%, content beta-carotene 12.664mg/g in the thalline, content beta-carotene 2.132g/L in the fermented liquid.
Comparative Examples 3:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 3.It is as follows to analyze the fermented liquid result: thalline content 16.74%, content beta-carotene 12.718mg/g in the thalline, content beta-carotene 2.129g/L in the fermented liquid.
Embodiment 4:
Substitute the liquid of clear water with the 8th time the fermented waste fluid of circulating, toward wherein adding starch 7% (weight), dregs of rice cake powder 2.8% (weight), vegetables oil 5.1% (weight), potassium primary phosphate 0.09% (weight), Triton (Triton) 0.20% (weight), vitamins B as the preparation substratum
110ppm, manganous sulfate 0.01% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 144 hours fermented liquid, analytical results is as follows: thalline content 15.06%, content beta-carotene 15.642mg/g in the thalline, content beta-carotene 2.355g/L in the fermented liquid.
Comparative Examples 4:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 4.It is as follows to analyze the fermented liquid result: thalline content 15.39%, content beta-carotene 10.424mg/g in the thalline, content beta-carotene 1.604g/L in the fermented liquid.
Embodiment 5:
Substitute the liquid of clear water with the 10th time the fermented waste fluid of circulating, toward wherein adding starch 6% (weight), dregs of rice cake powder 2.1% (weight), vegetables oil 6.0% (weight), potassium primary phosphate 0.06% (weight), Triton (Triton) 0.12% (weight), vitamins B as the preparation substratum
16ppm, manganous sulfate 0.06% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 168 hours fermented liquid, analytical results is as follows: thalline content 14.95%, content beta-carotene 16.470mg/g in the thalline, content beta-carotene 2.462g/L in the fermented liquid.
Comparative Examples 5:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 5.It is as follows to analyze the fermented liquid result: thalline content 14.75%, content beta-carotene 11.255mg/g in the thalline, content beta-carotene 1.660g/L in the fermented liquid.
Claims (2)
1, a kind of recycle fermented waste fluid is produced the novel process of natural beta-carotin, its production stage comprises that mainly employing blakeslea trispora (Blakeslea trispora) is through slant culture, seed culture, fermentation procedure, it is characterized in that described fermentation procedure is to utilize the mycelial waste liquid preparation of fermentation back filtering substratum to come preparing natural beta-carotin by fermentation, described fermention medium consists of starch 5～8% (weight), dregs of rice cake powder 1.2～2.8% (weight), vegetables oil 5.1～7.0% (weight), potassium primary phosphate 0.04～0.09% (weight), nonionogenic tenside alkylphenol polyoxyethylene 0.05～0.20% (weight), vitamins B
13～10ppm, manganous sulfate 0.01～0.06% (weight), the mycelial waste liquid of back filtering that will ferment again mixes with said components, and insufficient amount of liquid adds clear water to be supplied, and promptly can be used as the substratum of preparing natural beta-carotin by fermentation after stirring.
2, novel process as claimed in claim 1, the condition that it is characterized in that described fermentation culture are 26～28 ℃ of culture temperature, incubation time 120～168 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001166999A CN1163618C (en) | 2000-06-22 | 2000-06-22 | Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001166999A CN1163618C (en) | 2000-06-22 | 2000-06-22 | Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1331344A CN1331344A (en) | 2002-01-16 |
| CN1163618C true CN1163618C (en) | 2004-08-25 |
Family
ID=4586101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001166999A Expired - Fee Related CN1163618C (en) | 2000-06-22 | 2000-06-22 | Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1163618C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478405B (en) * | 2013-06-13 | 2015-02-25 | 河南科技大学 | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste |
| CN104131044B (en) * | 2014-06-13 | 2016-09-28 | 顾慧雅 | A kind of recycle the biomass after microorganism carries oil to the method producing unsaturated fatty acid |
| CN104694607B (en) * | 2015-03-12 | 2018-05-25 | 中国药科大学 | A kind of method that solid state fermentation prepares beta carotene |
| CN105368909A (en) * | 2015-12-15 | 2016-03-02 | 天津北洋百川生物技术有限公司 | Method for increasing yield of beta-carotene |
| CN107674897A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in bata-carotene zymotic fluid |
| CN111362445B (en) * | 2020-04-29 | 2021-01-05 | 吉林中粮生化有限公司 | Method and system for treating polyhydroxyalkanoate fermentation liquor by using adsorbent and application of obtained fermentation waste liquor |
| CN117487868A (en) * | 2023-12-28 | 2024-02-02 | 保龄宝生物股份有限公司 | Method for co-producing 2' -fucosyllactose by using D-psicose fermentation supernatant |
-
2000
- 2000-06-22 CN CNB001166999A patent/CN1163618C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1331344A (en) | 2002-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5464539A (en) | Process for the production of hydrogen by microorganisms | |
| Friel et al. | Production of a liquid inoculum/spawn of Agaricus bisporus | |
| CN1163618C (en) | Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid | |
| CN108298701A (en) | A kind of fermentation waste water processing method of low biodegradability after Anaerobic Treatment | |
| CN102250805A (en) | High-density culture method of composite microbial agent for papermaking wastewater treatment | |
| CN1063788C (en) | Fermenting production process of cordyceps fungus | |
| CN1916155A (en) | Method for preparing fermention medium, and producing biological pesticide by using wastewater from fermentation | |
| CN107760605A (en) | A kind of research method that tofu wastewater is purified using microdisk electrode | |
| CN109266578B (en) | Escherichia coli ACThr1032 and application thereof in fermentation production of L-threonine | |
| CN1054398C (en) | Bacillus capable of producing super oxide dismutase and its production method | |
| CN110791462A (en) | Bacillus subtilis and application thereof in fermentation production of adenosine | |
| Hamer | Lysis and “cryptic” growth in wastewater and sludge treatment processes | |
| CN1153823A (en) | Fermentative production of vitamin B6 | |
| CN1693450A (en) | Formulating and manufacturing method of culture medium for aerobic high temperature compost fermenation function bacteria | |
| CN1524961A (en) | Microorganism continuous catalysis method for producing acrylamide | |
| CN1120664C (en) | Process for preparing microbe insecticide with high-concentration waste organic water in fermentation industry | |
| CN1035681C (en) | Process for treating waste liquor of distiller's grain of alcohol | |
| CN1304583C (en) | Method for preparing dihydroxy acetone | |
| CN1017350B (en) | Production method of long chain alpha omega-dicarboxylic acid using microbe to ferment normal paraffin hydrocarbon | |
| CN1124349C (en) | Process for preparing arabitol by transforming glucose with yeast cells | |
| CN1023409C (en) | Nutritive liquid | |
| CN1053697C (en) | Fermentation method for producing D-ribose novel strain, and method for prepn. of D-ribose using said strain | |
| CN1290778C (en) | Culture method of microorganism for treating high temperature wastewater | |
| CN85100412A (en) | Produce the method for fixed glucose isomerase and be used for the production method of high fructose syrup with thermoactinomyces | |
| JPH08256782A (en) | Production of bacterium flocculant by fermentation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20040825 |