CN1331344A - Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid - Google Patents
Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid Download PDFInfo
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- CN1331344A CN1331344A CN00116699A CN00116699A CN1331344A CN 1331344 A CN1331344 A CN 1331344A CN 00116699 A CN00116699 A CN 00116699A CN 00116699 A CN00116699 A CN 00116699A CN 1331344 A CN1331344 A CN 1331344A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
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Abstract
A process for preparing natural beta-carotin by cyclic use of waste fermented, liquid includes slant culture, seed culture and fermentation, and features that in the fermentation step, the waste fermented liquid after the mycelia have been filtered out is used to replace clean water to prepare culture medium. Its advantages are environment protection and high effect.
Description
The present invention relates to a kind of technology of preparing natural beta-carotin by fermentation, relate to the novel process that a kind of recycle fermented waste fluid is produced natural beta-carotin or rather.
In the prior art, " biotechnology " 1998 the 8th the 5th phases of volume have been reported a kind of method of utilizing gourmet powder waste water to cultivate Bacillus thuringiensis, the waste water that this method utilizes the monosodium glutamate industrial fermentation to produce is raw material, preparation culture medium culturing Bacillus thuringiensis, determined optimum culture medium prescription, illustrated with gourmet powder waste water and cultivate Bacillus thuringiensis and then produce B
tBiological pesticide is feasible technically.But this technology also rests on laboratory stage at present.
" food and fermentation industries " the 24th the 5th phase of volume has been reported a kind of method of utilizing monosodium glutamate concentrating spent liquor production of protein feed by solid fermentation, test is found, but solid fermentation is produced animal feeding-stuff containing somatic protein after being concentrated into 38% gourmet powder waste water and partial supplementary material being mixed, confirm through the animal rearing test, tropina can replace bean-dregs feed, be suitable for raising livestock and poultry, economic benefit is obvious.
The waste liquid amount of fermentative Production natural beta-carotin generation now generally accounts for 75～80% of fermented liquid total amount, main more residual thalline do not have the nutritive substance that utilized in the waste liquid, wherein total reducing sugar is 0.9～3.6%, reducing sugar is 0.01～0.65%, amino nitrogen also has some micro-manganese metals etc. at 0.20～0.65mg/mL.Fermented waste fluid has features such as concentration height, colourity are big, solid content height, wherein COD
CrValue is up to about 17～220,000 mg/L, and suspended solids (SS) amount is very high, is milk sap, and colourity reaches 40000 times, is the organic waste water of high density, can not directly discharge, and must pass through and could discharge after environmental protection treatment reach national environmental standard.At present the organic waste water resolution recommended of country cuts both ways, though the treatment effect that has is better, complex process invest too greatly, and application economy does not pass a test on the suitability for industrialized production, has restricted the popularization of technology.Therefore, seeking the recycle of fermented waste fluid, realize the zero release of fermented waste fluid, is one of preferred approach that solves organic waste water improvement.
The purpose of this invention is to provide a kind of recycle fermented waste fluid and produce the novel process of natural beta-carotin, the fermented waste fluid of this technology by the recycle producing beta-carotene by fermentation is as the raw material of preparation substratum, reduced the treatment capacity of organic waste water, reduce facility investment, had good economic benefit and social benefit.
The present invention realizes like this.A kind of recycle fermented waste fluid of the present invention is produced the novel process of natural beta-carotin, the production stage of this production technique mainly comprises slant culture, seed culture, fermentation procedure, it is characterized in that described fermentation procedure is to utilize the mycelial waste liquid preparation of fermentation back filtering substratum to come preparing natural beta-carotin by fermentation.
The waste liquid preparation substratum of described utilization fermentation after-filtration comes preparing natural beta-carotin by fermentation, exactly preceding one time fermentation after-filtration is gone out mycelial waste collection and get up to prepare new fermented liquid, wherein the add-on of carbon source can be done suitable adjustment according to total reducing sugar amount residual in the waste liquid, and other nutritive ingredient can remain unchanged.
The bacterial classification of fermentation adopts produces the best bacterial strain of suitability for industrialized production---the blakeslea trispora (Blakeslea trispora) that natural beta-carotin is generally acknowledged in the world, wherein the slant culture of bacterial classification and seed culture adopt common cultural method, can adopt the cultivation respectively or the mixed culture of positive and negative bacterial strain.Promptly insert fermentation procedure after seed culture finishes and carry out fermentation culture.
Novel process of the present invention, described fermention medium consist of starch 5～8% (weight), dregs of rice cake powder 1.2～2.8% (weight), vegetables oil 5.1～7.0% (weight), potassium primary phosphate 0.04～0.09% (weight), nonionogenic tenside alkylphenol polyoxyethylene 0.05～0.20% (weight), vitamins B
13～10ppm, manganous sulfate 0.01～0.06% (weight), the mycelial waste liquid of back filtering that will ferment again mixes with said components, and insufficient amount of liquid adds clear water to be supplied, and promptly can be used as the substratum of preparing natural beta-carotin by fermentation after stirring.
Novel process of the present invention, described fermentation culture conditions are 26～28 ℃ of culture temperature, incubation time 120～168 hours.
The present invention compared with prior art has the following advantages:
One, the present invention is owing to adopt the recycle fermented waste fluid to produce the novel process of natural beta-carotin, solved the environmental protection treatment problem of the high concentrated organic waste liquid of preparing natural beta-carotin by fermentation generation, be the friendly process of a kind of fermented waste fluid near zero release, economic and social benefit is obvious.
Two, novel process of the present invention has been saved the expensive environmental protection treatment expense of fermented waste fluid and numerous and diverse treatment process, has saved the investment of a large amount of three wastes treatment facilities, has avoided the pollution of fermented waste fluid to environment.
Three, novel process of the present invention confirms through test of many times, and the fermented waste fluid preparation substratum of recycle preparing natural beta-carotin by fermentation is to the not influence of fermentation level of β-Hu Luobusu.During as fermented waste fluid recycle the 6th time, the fermentation level of β-Hu Luobusu is 2.307g/L, and contrast (preparing substratum with clear water) fermentation level is 2.350g/L; During fermented waste fluid recycle the 8th time, the fermentation level of β-Hu Luobusu is 2.436g/L, and the control fermentation level is 2.150g/L.
Further set forth the present invention below by detailed description, but embodiment not a limitation of the present invention to the specific embodiment of the invention.
Embodiment 1:
Fermented waste fluid after fermentation is filtered is for the first time substituted the liquid of clear water as the preparation substratum, toward wherein adding starch 5% (weight), dregs of rice cake powder 1.4% (weight), vegetables oil 6.6% (weight), potassium primary phosphate 0.06% (weight), Triton (Triton) 0.12% (weight), vitamins B
15ppm, manganous sulfate 0.05% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 120 hours fermented liquid, analytical results is as follows: thalline content 18.33%, content beta-carotene 12.097mg/g in the thalline, content beta-carotene 2.218g/L in the fermented liquid.
Comparative Examples 1:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 1.It is as follows to analyze the fermented liquid result: thalline content 16.55%, content beta-carotene 11.430mg/g in the thalline, content beta-carotene 1.892g/L in the fermented liquid.
Embodiment 2:
Except that fermented incubation time is 168 hours, other such as fermentative medium formula and compound method, access bacterial classification amount and culture condition with embodiment 1.It is as follows to analyze the fermented liquid result: thalline content 18.26%, content beta-carotene 12.458mg/g in the thalline, content beta-carotene 2.274g/L in the fermented liquid.
Comparative Examples 2:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 2.It is as follows to analyze the fermented liquid result: thalline content 17.43%, content beta-carotene 11.219mg/g in the thalline, content beta-carotene 1.955g/L in the fermented liquid.
Embodiment 3:
Fermented waste fluid with circulation the 5th substitutes the liquid of clear water as the preparation substratum, toward wherein adding starch 8% (weight), dregs of rice cake powder 1.2% (weight), vegetables oil 7.0% (weight), potassium primary phosphate 0.04% (weight), Triton (Triton) 0.05% (weight), vitamins B
13ppm, manganous sulfate 0.03% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 144 hours fermented liquid, analytical results is as follows: thalline content 16.84%, content beta-carotene 12.664mg/g in the thalline, content beta-carotene 2.132g/L in the fermented liquid.
Comparative Examples 3:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 3.It is as follows to analyze the fermented liquid result: thalline content 16.74%, content beta-carotene 12.718mg/g in the thalline, content beta-carotene 2.129g/L in the fermented liquid.
Embodiment 4:
Substitute the liquid of clear water with the 8th time the fermented waste fluid of circulating, toward wherein adding starch 7% (weight), dregs of rice cake powder 2.8% (weight), vegetables oil 5.1% (weight), potassium primary phosphate 0.09% (weight), Triton (Triton) 0.20% (weight), vitamins B as the preparation substratum
110ppm, manganous sulfate 0.01% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 144 hours fermented liquid, analytical results is as follows: thalline content 15.06%, content beta-carotene 15.642mg/g in the thalline, content beta-carotene 2.355g/L in the fermented liquid.
Comparative Examples 4:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 4.It is as follows to analyze the fermented liquid result: thalline content 15.39%, content beta-carotene 10.424mg/g in the thalline, content beta-carotene 1.604g/L in the fermented liquid.
Embodiment 5:
Substitute the liquid of clear water with the 10th time the fermented waste fluid of circulating, toward wherein adding starch 6% (weight), dregs of rice cake powder 2.1% (weight), vegetables oil 6.0% (weight), potassium primary phosphate 0.06% (weight), Triton (Triton) 0.12% (weight), vitamins B as the preparation substratum
16ppm, manganous sulfate 0.06% (weight) when amount of liquid is not enough, adds clear water and supplies amount of liquid, and regulating the pH value is 7.0.The positive and negative bacterium mixed seeds of blakeslea trispora (Blakesleatrispora) liquid is inserted in the fermention medium sterilization back that is mixed with, about 27 ℃, rotating speed be after the top fermentation of 230rpm shaking table is cultivated 168 hours fermented liquid, analytical results is as follows: thalline content 14.95%, content beta-carotene 16.470mg/g in the thalline, content beta-carotene 2.462g/L in the fermented liquid.
Comparative Examples 5:
Except that the preparation fermention medium substitutes the fermented waste fluid with clear water, other such as culture medium prescription, insert bacterial classification amount and culture condition with embodiment 5.It is as follows to analyze the fermented liquid result: thalline content 14.75%, content beta-carotene 11.255mg/g in the thalline, content beta-carotene 1.660g/L in the fermented liquid.
Claims (3)
1, a kind of recycle fermented waste fluid is produced the novel process of natural beta-carotin, its production stage mainly comprises slant culture, seed culture, fermentation procedure, it is characterized in that described fermentation procedure is to utilize the mycelial waste liquid preparation of fermentation back filtering substratum to come preparing natural beta-carotin by fermentation.
2, novel process as claimed in claim 1 is characterized in that described fermention medium consists of starch 5～8% (weight), dregs of rice cake powder 1.2～2.8% (weight), vegetables oil 5.1～7.0% (weight), potassium primary phosphate 0.04～0.09% (weight), nonionogenic tenside alkylphenol polyoxyethylene 0.05～0.20% (weight), vitamins B
13～10ppm, manganous sulfate 0.01～0.06% (weight), the mycelial waste liquid of back filtering that will ferment again mixes with said components, and insufficient amount of liquid adds clear water to be supplied, and promptly can be used as the substratum of preparing natural beta-carotin by fermentation after stirring.
3, novel process as claimed in claim 1, the condition that it is characterized in that described fermentation culture are 26～28 ℃ of culture temperature, incubation time 120～168 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB001166999A CN1163618C (en) | 2000-06-22 | 2000-06-22 | Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid |
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| CNB001166999A CN1163618C (en) | 2000-06-22 | 2000-06-22 | Process for preparing natural beta-carotin by cyclic utilization of fermented waste liquid |
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| CN1331344A true CN1331344A (en) | 2002-01-16 |
| CN1163618C CN1163618C (en) | 2004-08-25 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478405A (en) * | 2013-06-13 | 2014-01-01 | 河南科技大学 | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste |
| CN104131044A (en) * | 2014-06-13 | 2014-11-05 | 顾慧雅 | Method for recycling and utilizing biological substances after microbial oil extraction to produce unsaturated fatty acids |
| CN104694607A (en) * | 2015-03-12 | 2015-06-10 | 中国药科大学 | Method for preparing beta-carotin by solid fermentation |
| CN105368909A (en) * | 2015-12-15 | 2016-03-02 | 天津北洋百川生物技术有限公司 | Method for increasing yield of beta-carotene |
| CN107674897A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in bata-carotene zymotic fluid |
| CN111362445A (en) * | 2020-04-29 | 2020-07-03 | 吉林中粮生化有限公司 | Method and system for treating polyhydroxyalkanoate fermentation liquor by using adsorbent and application of obtained fermentation waste liquor |
| CN117487868A (en) * | 2023-12-28 | 2024-02-02 | 保龄宝生物股份有限公司 | Method for co-producing 2' -fucosyllactose by using D-psicose fermentation supernatant |
-
2000
- 2000-06-22 CN CNB001166999A patent/CN1163618C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478405A (en) * | 2013-06-13 | 2014-01-01 | 河南科技大学 | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste |
| CN103478405B (en) * | 2013-06-13 | 2015-02-25 | 河南科技大学 | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste |
| CN104131044A (en) * | 2014-06-13 | 2014-11-05 | 顾慧雅 | Method for recycling and utilizing biological substances after microbial oil extraction to produce unsaturated fatty acids |
| CN104694607A (en) * | 2015-03-12 | 2015-06-10 | 中国药科大学 | Method for preparing beta-carotin by solid fermentation |
| CN104694607B (en) * | 2015-03-12 | 2018-05-25 | 中国药科大学 | A kind of method that solid state fermentation prepares beta carotene |
| CN105368909A (en) * | 2015-12-15 | 2016-03-02 | 天津北洋百川生物技术有限公司 | Method for increasing yield of beta-carotene |
| CN107674897A (en) * | 2016-09-30 | 2018-02-09 | 青岛大学 | One kind is by method for extracting proteins in bata-carotene zymotic fluid |
| CN111362445A (en) * | 2020-04-29 | 2020-07-03 | 吉林中粮生化有限公司 | Method and system for treating polyhydroxyalkanoate fermentation liquor by using adsorbent and application of obtained fermentation waste liquor |
| CN117487868A (en) * | 2023-12-28 | 2024-02-02 | 保龄宝生物股份有限公司 | Method for co-producing 2' -fucosyllactose by using D-psicose fermentation supernatant |
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| Publication number | Publication date |
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| CN1163618C (en) | 2004-08-25 |
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Granted publication date: 20040825 |