CN116328602A - Fermentation device for microbial cultivation - Google Patents
Fermentation device for microbial cultivation Download PDFInfo
- Publication number
- CN116328602A CN116328602A CN202310220712.3A CN202310220712A CN116328602A CN 116328602 A CN116328602 A CN 116328602A CN 202310220712 A CN202310220712 A CN 202310220712A CN 116328602 A CN116328602 A CN 116328602A
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- distinguishing
- stirring
- control
- connecting rod
- gear
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- 238000000855 fermentation Methods 0.000 title claims abstract description 21
- 230000004151 fermentation Effects 0.000 title claims abstract description 21
- 230000000813 microbial effect Effects 0.000 title claims description 5
- 238000003756 stirring Methods 0.000 claims abstract description 132
- 244000005700 microbiome Species 0.000 claims abstract description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 abstract description 24
- 239000000463 material Substances 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract description 2
- 150000002500 ions Chemical class 0.000 abstract description 2
- 239000013049 sediment Substances 0.000 abstract description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 21
- 239000000126 substance Substances 0.000 description 12
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 11
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 11
- 229910052791 calcium Inorganic materials 0.000 description 11
- 239000011575 calcium Substances 0.000 description 11
- 229910052742 iron Inorganic materials 0.000 description 11
- 229910052749 magnesium Inorganic materials 0.000 description 11
- 239000011777 magnesium Substances 0.000 description 11
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 10
- 229940085991 phosphate ion Drugs 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 239000002738 chelating agent Substances 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000011229 interlayer Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- MIDXCONKKJTLDX-UHFFFAOYSA-N 3,5-dimethylcyclopentane-1,2-dione Chemical compound CC1CC(C)C(=O)C1=O MIDXCONKKJTLDX-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000002337 glycosamines Chemical class 0.000 description 1
- -1 iron ions Chemical class 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/40—Mixers with shaking, oscillating, or vibrating mechanisms with an axially oscillating rotary stirrer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
- B01F31/441—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing a rectilinear reciprocating movement
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/70—Drives therefor, e.g. crank mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
- B01F35/32—Driving arrangements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The utility model discloses a fermentation device for culturing microorganisms, which relates to the technical field of microorganism culture and comprises a bottom plate, a distinguishing component, a control component and a stirring component, wherein materials containing different ions can be respectively sterilized by arranging the distinguishing component, so that sediment is prevented from being formed under the high-temperature condition to further influence the microorganism culture; the control component is arranged to adjust the pressure and the flow rate of the gas when the sterilizing gas is sterilized, so that the quality and the efficiency of the sterilization are ensured; through setting up stirring subassembly can carry out quick abundant mixing after multiple material disinfection, guarantee that the material after the disinfection does not receive secondary pollution.
Description
Technical Field
The utility model relates to the technical field of microorganism culture, in particular to a fermentation device for microorganism culture.
Background
To obtain a pure culture of microorganisms, contamination by bacteria must be avoided, so that the equipment and workplace used are disinfected and sterilized. The culture medium is subjected to severe sterilization. The culture medium is sterilized by high pressure steam, and the culture medium is maintained at 121.3 ℃ for 15-30 min at 1.05kg/cm < 2 >. For sterilization. During the high-pressure steam sterilization process, certain thermolabile substances are destroyed by long-time high temperature, such as amino sugar and caramel are formed by sugar substances, so that the sugar-containing culture medium is usually sterilized at the temperature of 112.6 ℃ and 0.56kg/cm < 2 >, and certain culture media with high sugar requirements can be subjected to filtration sterilization or intermittent sterilization firstly and then mixed with other sterilized components; the high temperature can also cause carbonate to combine with certain cations (especially calcium, magnesium and iron ions) to form insoluble complexes to generate precipitation, thus affecting the culture of microorganisms; therefore, in preparing a synthetic medium for observing and quantitatively determining the growth condition of microorganisms, it is necessary to sterilize components containing calcium, magnesium, iron, etc. with phosphates, carbonates, respectively, and then mix them to avoid formation of precipitates; in the prior art, chelating agents are mostly added to avoid precipitation, but the chelating agents are generally harmful to skin, and after long-term use, the chelating agents can cause irritation to eyes and respiratory systems to cause asthma; in addition, the pressure and the flow rate of the sterilizing gas cannot be regulated during high-temperature sterilization in the prior art, and various different substances cannot be sterilized at the same time.
The utility model patent with publication number of CN210340917U discloses a fermentation device for microorganism strain culture, which comprises a tank body, wherein a feeding pipe, a driving device and a pressure regulating device are arranged at the top of the tank body, an annular spray pipe, a stirring shaft and stirring blades are arranged in the tank body, a plurality of spray holes are uniformly formed in the annular spray pipe, a heat source inlet pipe, a heat source outlet pipe and a liquid inlet pipe are respectively arranged on the side wall of the tank body, the heat source inlet pipe and the heat source outlet pipe are respectively communicated with a heating interlayer, the pressure regulating device is used for regulating the internal pressure of the tank body, and a discharge pipe is arranged at the bottom of the tank body. The utility model utilizes the cooperation of the heat source, the heating interlayer and the heating unit to keep the heating interlayer and the fermentation environment in the tank body constant temperature, and simultaneously can reduce the consumption of the heat source; meanwhile, the internal environment of the tank body is pressurized through the pressure regulating device to realize a constant-temperature and high-pressure fermentation environment, but the prior art fails to solve the problem that the components containing calcium, magnesium and iron plasma and the components containing phosphate plasma are required to be sterilized respectively during high-temperature sterilization, fails to solve the problem that chelating agents are required to be added to avoid precipitation, and fails to solve the problem that the pressure and the flow rate of sterilizing gas cannot be regulated during high-temperature sterilization.
Disclosure of Invention
Aiming at the technical problems, the utility model adopts the following technical scheme: the fermentation device for microbial cultivation comprises a bottom plate, a distinguishing component, a control component and a stirring component, wherein the control component is symmetrically arranged on two sides of the stirring component; the distinguishing assembly comprises distinguishing ratchets symmetrically arranged on two sides of the stirring assembly, a distinguishing rotating shaft is fixedly arranged on the distinguishing ratchets, a distinguishing gear ring is rotatably arranged on the distinguishing rotating shaft, and a plurality of distinguishing movable sheets are movably arranged on the distinguishing gear ring; the distinguishing movable piece is in sliding fit with the distinguishing ratchet wheel; the distinguishing gear ring is meshed with a distinguishing rack arranged on the distinguishing fixed frame in a sliding manner, the distinguishing rack is in sliding fit with a distinguishing sliding rod arranged on the distinguishing fixed frame in a sliding manner, the distinguishing sliding rod is in sliding fit with a distinguishing toggle rod arranged on the distinguishing fixed frame in a rotating manner, and a distinguishing reset spring I is movably arranged between the distinguishing sliding rod and the distinguishing fixed frame; the distinguishing rack is characterized in that a distinguishing placing box is fixedly arranged at one end of the distinguishing rack, a distinguishing reset spring II is movably arranged between the distinguishing rack and the distinguishing fixing frame, a distinguishing rotating frame is rotatably arranged on the distinguishing placing box, and a distinguishing cover plate is movably arranged on the distinguishing rotating frame.
Further, the distinguishing placement box is connected to the control assembly through a guide pipe, the control assembly comprises a control pressurizing cavity connected to the other side of the guide pipe, a control rack I is slidably arranged in the control pressurizing cavity, and the control rack I is meshed with a control meshing half gear movably arranged on the control fixing support; the control meshing half gear is rotatably provided with a first control connecting rod, the first control connecting rod is rotatably provided with a control fixing plate, and the control fixing plate is fixedly arranged on the distinguishing rotating shaft.
Further, the control meshing semi-gear is meshed with a second control rack which is slidably arranged in the control fixing support, one side of the second control rack is rotatably provided with a control handle, the control handle is in threaded fit with the control fixing support, one side of the control pressurizing cavity is fixedly provided with a control sterilizing tank, and the control sterilizing tank is fixedly arranged on the bottom plate.
Further, the stirring assembly is fixedly arranged on the bottom plate and comprises a stirring fixing plate fixedly arranged on the bottom plate, a stirring gear II and a stirring gear I are rotatably arranged on the stirring fixing plate, a stirring sheet is slidably arranged on the stirring gear II, and a stirring bevel gear is rotatably arranged on the stirring sheet.
Further, the stirring helical gear is meshed with a stirring gear III which is rotatably arranged on the stirring fixed disc, a stirring connecting rod I is fixedly arranged on the stirring gear III, a stirring connecting rod is rotatably arranged on the stirring connecting rod I, and the stirring connecting rod is rotatably arranged on a stirring connecting rod II.
Further, the stirring connecting rod II is fixedly arranged on the stirring worm wheel, the stirring worm wheel is rotationally arranged on the stirring fixed disc, the stirring worm wheel is meshed with the stirring worm fixedly arranged on the stirring gear I, and the stirring gear I is fixedly arranged on an output shaft of the stirring motor.
Further, the distinguishing poking rod is rotatably provided with a distinguishing pulling connecting rod, the distinguishing pulling connecting rod is rotatably arranged on the distinguishing connecting rod, the distinguishing connecting rod is rotatably arranged on the fermentation container, and the distinguishing rotating frame is in sliding fit with the distinguishing mixing pipe fixedly arranged on the bottom plate.
Further, the distinguishing connecting rod is in sliding fit with the distinguishing placement box.
Compared with the prior art, the utility model has the beneficial effects that: 1. according to the utility model, the material containing different ions can be disinfected respectively by arranging the distinguishing component, so that the problem that sediment is formed under the high temperature condition to influence the culture of microorganisms is avoided; 2. the control component is arranged to adjust the pressure and the flow rate of the gas when the sterilizing gas is sterilized, so that the quality and the efficiency of the sterilization are ensured; 3. through setting up stirring subassembly can carry out quick abundant mixing after multiple material disinfection, guarantee that the material after the disinfection does not receive secondary pollution.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model.
Fig. 2 is a schematic diagram of the whole structure of the present utility model in front view.
FIG. 3 is a schematic side view of the overall structure of the present utility model.
FIG. 4 is a schematic diagram illustrating a partial structure of a distinguishing component according to the present utility model.
FIG. 5 is a schematic diagram showing a partial structure of a distinguishing component according to the present utility model.
FIG. 6 is a schematic diagram of a distinguishing component according to the present utility model.
Fig. 7 is a schematic diagram of a partial structure of a control assembly according to the present utility model.
Fig. 8 is a schematic diagram of a partial structure of a control assembly according to the present utility model.
Fig. 9 is a partial cross-sectional view of a control assembly of the present utility model.
FIG. 10 is a schematic view of a part of the structure of the stirring assembly of the present utility model.
Reference numerals: 1-distinguishing components; 2-a control assembly; 3-a stirring assembly; 4-a bottom plate; 101-distinguishing gear rings; 102-differentiating ratchet; 103-distinguishing the movable sheet; 104-distinguishing racks; 105-distinguish the mount; 106-distinguishing a first reset spring; 107-distinguishing toggle bars; 108-distinguishing the sliding rod; 109-distinguish the second return spring; 110-distinguish placement boxes; 111-dividing the cover plate; 112-distinguish turret; 113-distinguishing the rotation axis; 114-distinguish pull links; 115-distinguish linkage; 116-distinguishing mixing tubes; 201-a control fixing plate; 202-controlling a disinfection tank; 203-control rack one; 204-control meshing half-gears; 205-control link one; 206-controlling the fixed bracket; 207-control rack two; 208-control handle; 209-controlling the pressurizing chamber; 301-a stirring motor; 302, stirring a fixed disc; 303-stirring gear one; 304-stirring gear II; 305-stirring helical gear; 306-stirring gear III; 307-stirring linkage; 308-stirring the first connecting rod; 309-stirring link two; 310-stirring worm wheel; 311-stirring worm; 312-stirring sheet.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10, a fermentation device for culturing microorganisms comprises a distinguishing component 1, a control component 2, a stirring component 3 and a bottom plate 4; the control components 2 are symmetrically arranged at two sides of the stirring component 3; the distinguishing assembly 1 comprises distinguishing ratchet wheels 102 symmetrically arranged at two sides of the stirring assembly 3, a distinguishing rotating shaft 113 is fixedly arranged on the distinguishing ratchet wheels 102, a distinguishing gear ring 101 is rotatably arranged on the distinguishing rotating shaft 113, and a plurality of distinguishing movable sheets 103 are movably arranged on the distinguishing gear ring 101; the distinguishing movable piece 103 is in sliding fit with the distinguishing ratchet 102; the distinguishing gear ring 101 is meshed with a distinguishing rack 104 which is arranged on a distinguishing fixed frame 105 in a sliding manner, the distinguishing rack 104 is in sliding fit with a distinguishing slide rod 108 which is arranged on the distinguishing fixed frame 105 in a sliding manner, the distinguishing slide rod 108 is in sliding fit with a distinguishing toggle rod 107 which is arranged on the distinguishing fixed frame 105 in a rotating manner, and a distinguishing reset spring I106 is movably arranged between the distinguishing slide rod 108 and the distinguishing fixed frame 105; a distinguishing placing box 110 is fixedly arranged at one end of the distinguishing rack 104, a distinguishing reset spring II 109 is movably arranged between the distinguishing rack 104 and the distinguishing fixing frame 105, a distinguishing rotating frame 112 is rotatably arranged on the distinguishing placing box 110, and a distinguishing cover plate 111 is movably arranged on the distinguishing rotating frame 112.
The distinguishing poking rod 107 is rotatably provided with a distinguishing pulling connecting rod 114, the distinguishing pulling connecting rod 114 is rotatably arranged on a distinguishing connecting rod 115, the distinguishing connecting rod 115 is rotatably arranged on the fermentation container, and the distinguishing rotating frame 112 is in sliding fit with a distinguishing mixing pipe 116 fixedly arranged on the bottom plate 4; the differentiation link 115 is in sliding engagement with the differentiation placement box 110.
The differential placement box 110 is connected to the control assembly 2 through a conduit, the control assembly 2 comprises a control pressurizing cavity 209 connected to the other side of the conduit, a first control rack 203 is slidably arranged in the control pressurizing cavity 209, and the first control rack 203 is meshed with a control meshing half gear 204 movably arranged on a control fixing bracket 206; a first control connecting rod 205 is rotatably arranged on the control meshing half gear 204, a control fixing plate 201 is rotatably arranged on the first control connecting rod 205, and the control fixing plate 201 is fixedly arranged on the distinguishing rotating shaft 113; the control meshing half gear 204 is meshed with a second control rack 207 which is slidably arranged in the control fixing support 206, one side of the second control rack 207 is rotatably provided with a control handle 208, the control handle 208 is in threaded fit with the control fixing support 206, one side of the control pressurizing cavity 209 is fixedly provided with a control sterilizing tank 202, and the control sterilizing tank 202 is fixedly arranged on the bottom plate 4.
The stirring assembly 3 is fixedly arranged on the bottom plate 4, the stirring assembly 3 comprises a stirring fixed disc 302 fixedly arranged on the bottom plate 4, a stirring gear II 304 and a stirring gear I303 are rotatably arranged on the stirring fixed disc 302, a stirring sheet 312 is slidably arranged on the stirring gear II 304, and a stirring bevel gear 305 is rotatably arranged on the stirring sheet 312; the stirring helical gear 305 is meshed with a stirring gear III 306 which is rotatably arranged on the stirring fixed disk 302, a stirring connecting rod I308 is fixedly arranged on the stirring gear III 306, a stirring connecting rod 307 is rotatably arranged on the stirring connecting rod I308, and the stirring connecting rod 307 is rotatably arranged on a stirring connecting rod II 309; the stirring link II 309 is fixedly arranged on a stirring worm wheel 310, the stirring worm wheel 310 is rotatably arranged on a stirring fixed disk 302, the stirring worm wheel 310 is meshed with a stirring worm 311 fixedly arranged on a stirring gear I303, and the stirring gear I303 is fixedly arranged on an output shaft of the stirring motor 301.
The utility model discloses a fermentation device for microbial cultivation, which has the working principle that: the substances of the components containing calcium, magnesium, iron, etc. and the components containing phosphate ions are respectively injected into the separate placement boxes 110 symmetrically disposed along the stirring member 3, after the two substances are injected to a predetermined weight.
The distinguishing poking rod 107 on one side of the stirring assembly 3 is manually poked to drive the distinguishing sliding rod 108 to enable the distinguishing sliding rod 108 to be separated from the distinguishing rack 104, the distinguishing rack 104 is pulled by the distinguishing placement box 110 to enable the distinguishing gear ring 101 to rotate to drive the distinguishing ratchet 102, the distinguishing rotating shaft 113 and the control fixing plate 201, the control connecting rod I205 is pulled when the control fixing plate 201 rotates, the control connecting rod I205 enables the control meshing half gear 204 to rotate, the control handle 208 is manually adjusted to enable the control rack II 207 to adjust the position of the control meshing half gear 204, and then the sliding distance of the control rack I203 in the control pressurizing cavity 209 is controlled to realize pressure control of sterilizing gas; controlling the conduit on the pressurizing cavity 209 to introduce the sterilizing gas into the distinguishing placement box 110 with the calcium, magnesium and iron plasma components on one side, and sterilizing substances with the calcium, magnesium and iron plasma components in the distinguishing placement box 110; meanwhile, when the distinguishing and placing box 110 filled with the calcium, magnesium and iron plasma components moves to a preset position, the distinguishing rotating frame 112 is in sliding fit with the distinguishing and mixing tube 116, so that the distinguishing rotating frame 112 drives the distinguishing cover plate 111, the distinguishing cover plate 111 is far away from the distinguishing and placing box 110, substances of the calcium, magnesium and iron plasma components in the distinguishing and placing box 110 are poured into the distinguishing and mixing tube 116, and the substances of the calcium, magnesium and iron plasma components enter the stirring container through the guide tube.
When the distinguishing box 110 containing the calcium, magnesium and iron plasma components touches the distinguishing connecting rod 115, the distinguishing connecting rod 115 pulls the distinguishing pulling connecting rod 114, the distinguishing pulling connecting rod 114 pulls the distinguishing poking rod 107 with the other side injected with the phosphate ion component, so that the distinguishing poking rod 107 on the side containing the phosphate ion component drives the distinguishing sliding rod 108 to separate from the distinguishing rack 104, the distinguishing rack 104 rotates the distinguishing gear ring 101 under the pulling of the distinguishing box 110 so as to drive the distinguishing ratchet 102, the distinguishing rotating shaft 113 and the control fixing plate 201, the control connecting rod one 205 is pulled when the control fixing plate 201 rotates, the control connecting rod one 205 rotates the control meshing half gear 204, the control handle 208 is manually adjusted so that the control rack two 207 adjusts the position of the control meshing half gear 204, and then the sliding distance of the control rack one 203 in the control pressurizing cavity 209 is controlled to realize the pressure control of the sterilizing gas; controlling the conduit on the pressurizing chamber 209 to introduce the sterilizing gas into the distinguishing box 110 at the side containing the phosphate ion component, and sterilizing the substance containing the phosphate ion component in the distinguishing box 110; meanwhile, when the distinguishing and placing box 110 containing phosphate ion components moves to a preset position, the distinguishing rotating frame 112 is in sliding fit with the distinguishing and mixing pipe 116, so that the distinguishing rotating frame 112 drives the distinguishing cover plate 111, the distinguishing cover plate 111 is far away from the distinguishing and placing box 110, substances containing phosphate ion components in the distinguishing and placing box 110 are poured into the distinguishing and mixing pipe 116, and the substances containing phosphate ion components enter the stirring container through the guide pipe.
When the substances containing calcium, magnesium, iron and phosphate ion are sterilized and enter the stirring container, the stirring motor 301 is started to drive the stirring gear I303, the stirring gear II 304 and the stirring worm 311, so that the stirring sheet 312 rotates and stirs, and meanwhile, the stirring worm 311 drives the stirring worm wheel 310, the stirring connecting rod II 309, the stirring connecting rod 307, the stirring connecting rod I308 and the stirring gear III 306 to enable the stirring sheet 312 to move up and down and stir more uniformly.
Claims (8)
1. Fermentation device for microbial cultivation, comprising a bottom plate (4), characterized in that: the stirring device further comprises a distinguishing component (1), a control component (2) and a stirring component (3), wherein the control component (2) is symmetrically arranged at two sides of the stirring component (3); the distinguishing assembly (1) comprises distinguishing ratchets (102) symmetrically arranged on two sides of the stirring assembly (3), a distinguishing rotating shaft (113) is fixedly arranged on the distinguishing ratchets (102), a distinguishing gear ring (101) is rotatably arranged on the distinguishing rotating shaft (113), and a plurality of distinguishing movable sheets (103) are movably arranged on the distinguishing gear ring (101); the distinguishing movable piece (103) is in sliding fit with the distinguishing ratchet wheel (102); the distinguishing gear ring (101) is meshed with a distinguishing rack (104) arranged on the distinguishing fixed frame (105) in a sliding manner, the distinguishing rack (104) is in sliding fit with a distinguishing sliding rod (108) arranged on the distinguishing fixed frame (105) in a sliding manner, the distinguishing sliding rod (108) is in sliding fit with a distinguishing toggle rod (107) arranged on the distinguishing fixed frame (105) in a rotating manner, and a distinguishing reset spring I (106) is movably arranged between the distinguishing sliding rod (108) and the distinguishing fixed frame (105); the distinguishing rack (104) one end is fixedly provided with a distinguishing placing box (110), a distinguishing reset spring II (109) is movably arranged between the distinguishing rack (104) and the distinguishing fixing frame (105), a distinguishing rotating frame (112) is rotatably arranged on the distinguishing placing box (110), and a distinguishing cover plate (111) is movably arranged on the distinguishing rotating frame (112).
2. A fermentation device for use in the cultivation of microorganisms according to claim 1, wherein: the distinguishing placement box (110) is connected to the control assembly (2) through a conduit, the control assembly (2) comprises a control pressurizing cavity (209) connected to the other side of the conduit, a control rack I (203) is slidably arranged in the control pressurizing cavity (209), and the control rack I (203) is meshed with a control meshing half gear (204) movably arranged on the control fixing support (206); the control meshing half gear (204) is rotatably provided with a first control connecting rod (205), the first control connecting rod (205) is rotatably provided with a control fixing plate (201), and the control fixing plate (201) is fixedly arranged on the distinguishing rotating shaft (113).
3. A fermentation device for use in the cultivation of microorganisms according to claim 2, wherein: the control meshing semi-gear (204) is meshed with a control rack II (207) which is arranged in a control fixing support (206) in a sliding mode, a control handle (208) is arranged on one side of the control rack II (207) in a rotating mode, the control handle (208) is in threaded fit with the control fixing support (206), a control disinfection tank (202) is fixedly arranged on one side of a control pressurizing cavity (209), and the control disinfection tank (202) is fixedly arranged on a bottom plate (4).
4. A fermentation device for use in the cultivation of microorganisms according to claim 3, wherein: the stirring device is characterized in that a stirring assembly (3) is fixedly arranged on the bottom plate (4), the stirring assembly (3) comprises a stirring fixing plate (302) fixedly arranged on the bottom plate (4), a stirring gear II (304) and a stirring gear I (303) are rotatably arranged on the stirring fixing plate (302), a stirring piece (312) is slidably arranged on the stirring gear II (304), and a stirring bevel gear (305) is rotatably arranged on the stirring piece (312).
5. A fermentation device for the cultivation of microorganisms according to claim 4, wherein: the stirring helical gear (305) is meshed with a stirring gear III (306) which is rotatably arranged on the stirring fixed disc (302), a stirring connecting rod I (308) is fixedly arranged on the stirring gear III (306), a stirring connecting rod (307) is rotatably arranged on the stirring connecting rod I (308), and the stirring connecting rod (307) is rotatably arranged on a stirring connecting rod II (309).
6. A fermentation device for use in the cultivation of microorganisms according to claim 5, wherein: the stirring connection rod II (309) is fixedly arranged on the stirring worm wheel (310), the stirring worm wheel (310) is rotatably arranged on the stirring fixing disc (302), the stirring worm wheel (310) is meshed with the stirring worm (311) fixedly arranged on the stirring gear I (303), and the stirring gear I (303) is fixedly arranged on an output shaft of the stirring motor (301).
7. A fermentation device for use in the cultivation of microorganisms according to claim 1, wherein: the distinguishing toggle rod (107) is rotatably provided with a distinguishing pulling connecting rod (114), the distinguishing pulling connecting rod (114) is rotatably arranged on a distinguishing connecting rod (115), the distinguishing connecting rod (115) is rotatably arranged on a fermentation container, and the distinguishing rotating frame (112) is in sliding fit with a distinguishing mixing pipe (116) fixedly arranged on the bottom plate (4).
8. A fermentation device for use in the cultivation of microorganisms according to claim 7, wherein: the distinguishing connecting rod (115) is in sliding fit with the distinguishing placement box (110).
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| CN202310220712.3A CN116328602A (en) | 2023-03-09 | 2023-03-09 | Fermentation device for microbial cultivation |
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| CN202310220712.3A CN116328602A (en) | 2023-03-09 | 2023-03-09 | Fermentation device for microbial cultivation |
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