CN114317228B - Stem cell culture device and culture method thereof - Google Patents
Stem cell culture device and culture method thereof Download PDFInfo
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- CN114317228B CN114317228B CN202111430491.XA CN202111430491A CN114317228B CN 114317228 B CN114317228 B CN 114317228B CN 202111430491 A CN202111430491 A CN 202111430491A CN 114317228 B CN114317228 B CN 114317228B
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Abstract
The invention provides a stem cell culture device, which comprises a machine body, wherein a culture cavity is formed in the machine body and is communicated with the top wall of the machine body through a liquid feeding channel; cultivate the chamber roof rigid coupling and have L shaped plate and two pH value automatically regulated boxes that the structure is the same, L shaped plate and cultivate the chamber right wall and enclose into the transmission chamber, are equipped with the oxygen and supply the pipeline in the transmission chamber, and the oxygen is supplied pipeline and organism right wall connection, and oxygen and is supplied pipeline one end and extend to the organism outside. The invention can accurately regulate the pH value of the mixed liquid of the stem cells and the culture medium.
Description
Technical Field
The invention relates to the technical field of stem cell culture, in particular to a stem cell culture device and a culture method thereof.
Background
The stem cells are multipotential cells with self-replication ability, can be differentiated into various functional cells under certain conditions, are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are divided into three categories according to the development potentiality of the stem cells; the stem cell is an insufficiently differentiated and immature cell, has the potential function of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field.
The stem cell perfusion culture is that after stem cells and culture medium are added into a reactor together, part of the conditioned culture medium is continuously taken out in the processes of cell growth and product formation, and simultaneously, new culture medium is continuously perfused.
The current stem cell culture perfusion device cannot conveniently and accurately adjust the pH value of a culture medium and stem cells.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides a stem cell culture apparatus, which is implemented by adopting the following technical scheme:
a stem cell culture device comprises a machine body, wherein a culture cavity is formed in the machine body and is communicated with the top wall of the machine body through a liquid feeding channel, the culture cavity is communicated with the bottom wall of the machine body through a liquid outlet channel, and an electromagnetic valve is arranged in the liquid outlet channel;
cultivate the chamber roof rigid coupling and have L shaped plate and two pH value automatically regulated boxes that the structure is the same, L shaped plate and cultivate the chamber right wall and enclose into the transmission chamber, are equipped with oxygen supplementary pipeline in the transmission chamber, and oxygen supplementary pipeline and organism right wall are connected, and oxygen supplementary pipeline one end extends to the organism outside.
Beneficially, a liquid storage cavity is formed in the automatic pH value adjusting box body, the lower wall of the liquid storage cavity is communicated with the bottom wall of the automatic pH value adjusting box body through a liquid outlet hole, a first transmission groove and a second transmission groove are formed in the side wall of the automatic pH value adjusting box body, and the first transmission groove penetrates through the liquid outlet hole;
a first transmission strip is arranged in the first transmission groove, a connecting hole is formed in the first transmission strip, one end of the first transmission strip is connected with the end part of the first transmission groove through a first elastic piece, the other end of the first transmission strip is in a curved surface shape, and one end of the first transmission strip in the curved surface shape extends to the outside of the pH value automatic adjusting box body;
a second transmission strip is arranged in the second transmission groove, a first transmission block is arranged at one end of the second transmission strip, the first transmission block is hemispherical, a pressing strip is arranged at the other end of the second transmission strip, one end of the second transmission strip, which is provided with the pressing strip, is connected with the end part of the second transmission groove through a second elastic piece, a rubber head dropper is further arranged in the pH value automatic adjusting box body, a rubber cap of the rubber head dropper extends into the second transmission groove, the side wall of the rubber cap abuts against the end part of the second transmission groove, and a liquid outlet of the rubber head dropper extends to the bottom of the pH value automatic adjusting box body;
the inner frame of the culture cavity is provided with a bearing plate, the bearing plate is provided with two transmission mechanisms, each transmission mechanism comprises a motor, a rotary disc, a first transmission plate and a second transmission plate, the motors are fixedly connected to the bearing plate, the rotary disc is fixedly connected to a power output shaft of the motors, the first transmission plate and the second transmission plate are respectively fixedly connected to the top of the rotary disc, the first transmission plate and the second transmission plate are abutted, one side wall of the first transmission plate is a first inclined surface, one side wall of the second transmission plate is a second inclined surface, the other side wall of the second transmission plate is a third inclined surface, more than two notches are formed in the top of the second transmission plate, the rear wall of the culture cavity is slidably connected with two sliding mechanisms, each sliding mechanism comprises a sliding plate and a third elastic part, the upper end of the sliding plate is connected with the top wall of the culture cavity through the third elastic part, the side wall of the sliding plate is fixedly connected with a second transmission block, the second transmission block is hemispherical, and rollers are arranged at the bottom of the sliding plate.
Beneficially, the oxygen supply pipeline includes first pipeline and second pipeline, first pipeline rigid coupling in organism right side wall, first pipeline left end is the fourth inclined plane, and the second pipeline right-hand member extends to the organism outside, and first pipeline inner wall rigid coupling has the ultraviolet ray disinfection lamp, and the transmission chamber is put on the shelf and is equipped with the rotation axis, and the second pipeline rotates with the rotation axis to be connected, and second pipeline left end rigid coupling has the gasbag, gasbag diapire rigid coupling in the top of L shaped plate horizontal segment, second pipeline right-hand member are the fifth inclined plane, and fourth inclined plane and fifth inclined plane right-hand member offset.
Beneficially, the rear wall of the transmission cavity is fixedly connected with a rectangular ring, the inner wall of the rectangular ring is connected with a sliding block in a sliding mode, a first transmission rod and a second transmission rod are connected to the sliding block in a rotating mode, the lower end of the first transmission rod is hinged to the top of the second pipeline, the lower end of the second transmission rod is connected with a baffle in a rotating mode, and the lower end of the baffle extends into the first pipeline.
Beneficially, be equipped with processing module in the organism, cultivate the chamber bottom and be equipped with the pH value apparatus, cultivate the chamber top rigid coupling and have oxygen concentration sensor, the motor, solenoid valve, pH value apparatus and oxygen concentration sensor are connected with processing module electricity respectively.
Advantageously, the top of the body is provided with an openable and closable sealing cover which covers the upper end of the filling channel.
The invention also provides a culture method for culturing stem cells by using the stem cell culture device, which comprises the following steps:
the method comprises the following steps: adding stem cells and culture medium from the priming channel into the culture chamber;
step two: stirring the stem cells and the culture medium in the culture cavity to form a mixed solution;
step three: adjusting the pH value of the mixed solution through the two automatic pH value adjusting box bodies;
step four: oxygen is blown into the oxygen supplementing pipeline through an external oxygen generating device, so that a proper amount of oxygen is provided for stem cell culture in the culture cavity.
The invention has the following beneficial effects:
according to the invention, the pH value of the mixed liquid is automatically detected by the pH value tester, and the two motors are controlled by the processing module to control the rotating disc to rotate by different angles, so that the two pH value automatic adjusting box bodies perform coarse adjustment and accurate adjustment on the pH value of the mixed liquid, the pH value of the mixed liquid is controlled within a proper range, a proper acid-base environment is provided for stem cell culture, and the culture efficiency is improved; through oxygen concentration sensor automatic identification cultivates the oxygen concentration of intracavity, the ultraviolet ray disinfection lamp carries out the disinfection and sterilization preliminary treatment for the oxygen that will get into the cultivation chamber, and the oxygen that has disinfected in automatic messenger second pipeline and the gasbag supplyes the cultivation chamber through the gasbag and supplies stem cell to grow and use, and makes the baffle reciprocate through first transfer line, slider, second transfer line are automatic, realizes blockking that non-sterile oxygen directly gets into to cultivate the chamber and pollute stem cell.
Drawings
The invention is further illustrated by means of the attached drawings, but the embodiments in the drawings do not constitute any limitation to the invention, and for a person skilled in the art, other drawings can be obtained on the basis of the following drawings without inventive effort.
FIG. 1 is a schematic view showing the structure of a stem cell culture apparatus according to the present invention;
FIG. 2 is an enlarged view of the invention at A in FIG. 1;
fig. 3 is an enlarged view of the invention at B in fig. 1.
Reference numerals are as follows: the machine body 1, the culture cavity 2, the liquid feeding channel 3, the liquid outlet channel 4, the electromagnetic valve 5, the transmission cavity 6, the L-shaped plate 7, the pH value automatic adjusting box body 8, the liquid storage cavity 81, the liquid outlet hole 82, the first transmission groove 83, the second transmission groove 84, the second transmission strip 85, the first transmission block 851, the pressing strip 852, the second elastic element 853, the first transmission strip 86, the connection hole 861, the first elastic element 862, the rubber head dropper 87, the rubber cap 871, the liquid outlet 872, the support plate 9, the motor 10, the power output shaft 11, the rotary disk 12, the first transmission plate 13, the first inclined surface 131, the second transmission plate 14, the second inclined surface 141, the third inclined surface 142, the notch 143, the sliding plate 15, the second transmission block 151, the roller 152, the third elastic element 16, the first pipeline 17, the ultraviolet disinfection lamp 171, the fourth inclined surface 173, the second pipeline 18, the rotating shaft 181, the first transmission rod 182, the fifth inclined surface 183, the air bag 19 and the sliding block 20, the device comprises a rectangular loop 21, a second transmission rod 22, a baffle plate 23, a sealing cover 24, a pH value tester 25 and an oxygen concentration sensor 26.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, "first," "second," "third," and "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be further noted that, unless otherwise specifically stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, integrally connected, mechanically connected, electrically connected, directly connected, connected through an intermediate medium, or connected through the insides of two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
As shown in fig. 1-3, a stem cell culture device comprises a body 1, a culture chamber 2 is arranged in the body 1, the culture chamber 2 is communicated with the top wall of the body 1 through a liquid feeding channel 3, the culture chamber 2 is communicated with the bottom wall of the body 1 through a liquid outlet channel 4, and an electromagnetic valve 5 is arranged in the liquid outlet channel 4;
cultivate 2 roof rigid couplings in chamber and have L shaped plate 7 and two pH value automatically regulated boxes 8 that the structure is the same, L shaped plate 7 and cultivate 2 right walls in chamber and enclose into transmission chamber 6, are equipped with the oxygen in the transmission chamber 6 and supply the pipeline, and oxygen supplies pipeline and organism 1 right wall connection, and oxygen supplies pipeline one end and extends to organism 1 outside.
The solenoid valve 5 is an automated basic element for controlling fluid, and belongs to an actuator, the solenoid valve 5 is not limited to hydraulic or pneumatic, and the solenoid valve 5 is generally used in an industrial control system to adjust the direction, flow rate, speed, on-off and other parameters of a medium.
According to an optional embodiment of the present invention, a liquid storage cavity 81 is formed in the automatic pH adjusting box 8, a lower wall of the liquid storage cavity 81 is communicated with a bottom wall of the automatic pH adjusting box 8 through a liquid outlet 82, a first transmission groove 83 and a second transmission groove 84 are formed in a side wall of the automatic pH adjusting box 8, and the first transmission groove 83 penetrates through the liquid outlet 82;
a first transmission bar 86 is arranged in the first transmission groove 83, a connecting hole 861 is formed in the first transmission bar 86, one end of the first transmission bar 86 is connected with the end of the first transmission groove 83 through a first elastic piece 862, the other end of the first transmission bar 86 is curved, and one end of the first transmission bar 86, which is curved, extends to the outside of the pH value automatic adjusting box body 8;
a second transmission bar 85 is arranged in the second transmission groove 84, one end of the second transmission bar 85 is provided with a first transmission block 851, the first transmission block 851 is hemispherical, the other end of the second transmission bar 85 is provided with a pressing strip 852, one end of the second transmission bar 85 provided with the pressing strip 852 is connected with the end part of the second transmission groove 84 through a second elastic part 853, a rubber head dropper 87 is further arranged in the automatic pH value adjusting box body 8, a rubber cap 871 of the rubber head dropper 87 extends into the second transmission groove 84, the side wall of the rubber cap 871 is abutted against the end part of the second transmission groove 84, and a liquid outlet 872 of the rubber head dropper 87 extends to the bottom of the automatic pH value adjusting box body 8;
the culture cavity 2 is internally provided with a supporting plate 9, the supporting plate 9 is provided with two transmission mechanisms, each transmission mechanism comprises a motor 10, a rotating disc 12, a first transmission plate 13 and a second transmission plate 14, the motor 10 is fixedly connected on the supporting plate 9, the rotating disc 12 is fixedly connected on a power output shaft 11 of the motor 10, the first transmission plate 13 and the second transmission plate 14 are respectively and fixedly connected on the top of the rotating disc 12, the first transmission plate 13 is abutted against the second transmission plate 14, one side wall of the first transmission plate 13 is a first inclined plane 131, one side wall of the second transmission plate 14 is a second inclined plane 141, the other side wall of the second transmission plate 14 is a third inclined plane 142, the top of the second transmission plate 14 is provided with more than two notches 143, the rear wall of the culture cavity 2 is slidably connected with two sliding mechanisms, each sliding mechanism comprises a sliding plate 15 and a third elastic member 16, the upper end of the sliding plate 15 is connected with the top wall of the culture cavity 2 through the third elastic member 16, the side wall of the sliding plate 15 is fixedly connected with a second transmission block 151, the second transmission block 151 is formed in a hemispherical shape, and a roller 152 is provided at the bottom of the slide plate 15.
According to an optional embodiment of the present invention, the oxygen supply pipe includes a first pipe 17 and a second pipe 18, the first pipe 17 is fixedly connected to a right wall of the machine body 1, a left end of the first pipe 17 is a fourth inclined surface 173, a right end of the second pipe 18 extends to an outside of the machine body 1, an ultraviolet disinfection lamp 171 is fixedly connected to an inner wall of the first pipe 17, a rotating shaft 181 is erected on the transmission cavity 6, the second pipe 18 is rotatably connected to the rotating shaft 181, a gas bag 19 is fixedly connected to a left end of the second pipe 18, a bottom wall of the gas bag 19 is fixedly connected to a top of a horizontal section of the L-shaped plate 7, a fifth inclined surface 183 is arranged at a right end of the second pipe 18, and the fourth inclined surface 173 and the fifth inclined surface 183 are abutted.
According to an alternative embodiment of the present invention, a rectangular ring 21 is fixedly connected to the rear wall of the transmission cavity 6, a sliding block 20 is slidably connected to the inner wall of the rectangular ring 21, a first transmission rod 182 and a second transmission rod 22 are rotatably connected to the sliding block 20, the lower end of the first transmission rod 182 is hinged to the top of the second pipeline 18, the lower end of the second transmission rod 22 is rotatably connected to a baffle 23, and the lower end of the baffle 23 extends into the first pipeline 17.
According to an alternative embodiment of the present invention, a processing module is disposed in the machine body 1, a pH meter 25 is disposed at the bottom of the culture chamber 2, an oxygen concentration sensor 26 is fixedly connected to the top of the culture chamber 2, and the motor 10, the solenoid valve 5, the pH meter 25 and the oxygen concentration sensor 26 are electrically connected to the processing module respectively.
The processing module is a module with control and data analysis functions.
In an alternative embodiment of the invention, the top of the machine body 1 is provided with an openable and closable sealing cover 24, and the sealing cover 24 covers the upper end of the filling channel 3.
According to an alternative embodiment of the present invention, the method for culturing stem cells using the stem cell culture apparatus comprises the steps of:
the method comprises the following steps: stem cells and culture medium are added into the culture cavity 2 from the liquid adding channel 3;
step two: stirring the stem cells and the culture medium in the culture cavity 2 to form a mixed solution;
step three: the pH value of the mixed solution is adjusted through the two automatic pH value adjusting box bodies 8;
step four: oxygen is blown into the oxygen supply pipeline through an external oxygen generating device, so that a proper amount of oxygen is provided for stem cell culture in the culture cavity 2.
The implementation process comprises the following steps: the sealing cover 24 is opened, the stem cells and the culture medium are added into the culture cavity 2 from the liquid adding channel 3, the sealing cover 24 is closed, the stem cells and the culture medium in the culture cavity 2 are stirred to form a mixed liquid, ultrasonic stirring can be used for preventing the stem cells from being damaged, a user sets the amount of the added acid liquid or the alkali liquid according to the pH value of the mixed liquid measured by the pH value measuring instrument 25, the acid liquid is filled in the liquid storage cavity 81 of the left pH value automatic adjusting box body 8, and the alkali liquid is filled in the liquid storage cavity 81 of the right pH value automatic adjusting box body 8.
If the pH value of the mixed liquid needs to be adjusted to be small, the processing module controls the power output shaft 11 of the left motor 10 to rotate by a set angle, so as to drive the rotating disc 12, the first transmission plate 13 and the second transmission plate 14 to rotate, when the roller 152 on the left sliding plate 15 moves from the first inclined plane 131 to the top of the first transmission plate 13, the motor 10 stops operating, at this time, the second transmission block 151 pushes the first transmission bar 86 to the left, so that the acid liquid in the liquid storage cavity 81 in the liquid outlet 82 moves to the mixed liquid through the connecting hole 861, when the pH value of the mixed liquid approaches a preset value, the motor 10 is started again, the roller 152 moves to the top of the second transmission plate 14 through the second inclined plane 141, at this time, the first transmission bar 86 loses the thrust of the second transmission block 151 and moves to the right under the elastic force of the first elastic member 862, the liquid outlet 82 is closed by the first transmission bar 86, the acid liquid stops flowing out, when the roller 152 moves at the top of the third inclined plane 142, the roller 152 moves up and down along the plurality of notches 143 for a plurality of times under the elastic force of the third elastic member 16, therefore, the second transmission block 151 pushes the first transmission block 851 for multiple times, so that the second transmission strip 85 moves left and right in a reciprocating manner, the pressing strip 852 is used for extruding the rubber cap 871, acid liquid in the left rubber head dropper 87 is dripped into the mixed liquid from the liquid outlet 872, the roller 152 passes through the plurality of notches 143, and the rubber head dropper 87 can drip the acid liquid, so that the pH value of the mixed liquid can be accurately adjusted.
When the pH value of the mixed liquid needs to be adjusted to be large, the processing module controls the right motor 10 to be started by applying the same principle, the alkali liquid in the right liquid storage cavity 81 is dripped into the mixed liquid by using the movement of the first transmission strip 86, and the pH value of the mixed liquid is accurately adjusted by using the alkali liquid in the right rubber head dropper 87.
The oxygen concentration sensor 26 obtains the oxygen concentration in the culture chamber 2, when the oxygen concentration is insufficient, a user blows oxygen into the first pipeline 17 by using an external oxygen generating device, the oxygen slowly enters the second pipeline 18 and the air bag 19, the ultraviolet disinfection lamp 171 disinfects and sterilizes the oxygen staying in the second pipeline 18 and the air bag 19, when the oxygen in the air bag 19 gradually increases, the air bag 19 expands, the second pipeline 18 rotates clockwise around the rotating shaft 181, the second pipeline 18 drives the sliding block 20 to move right through the first transmission rod 182, the sliding block 20 drives the baffle plate 23 to move downwards through the second transmission rod 22, the baffle plate 23 prevents non-sterilized air from entering the transmission chamber 6, after the second pipeline 18 rotates, the sterilized oxygen in the air bag 19 and the second pipeline 18 can enter the culture chamber 2 to supplement the oxygen concentration, the oxygen in the air bag 19 shrinks after escaping, the second pipeline 18 returns to a horizontal state, the shutter 23 moves upward to wait for the next oxygen replenishment.
After the culture is finished, the processing module controls the electromagnetic valve 5 to discharge the mixed liquid out of the machine body 1 for subsequent operation.
The pH value of the mixed liquid is automatically detected by the pH value tester, and the two motors 10 are controlled by the processing module to control the rotating disc 12 to rotate by different angles, so that the two pH value automatic adjusting box bodies 8 can roughly adjust and accurately adjust the pH value of the mixed liquid, the pH value of the mixed liquid is controlled within an appropriate range, an appropriate acid-base environment is provided for stem cell culture, and the culture efficiency is improved; through the oxygen concentration sensor 26 automatic identification cultivate the interior oxygen concentration of chamber 2, ultraviolet ray disinfection lamp 171 carries out the disinfection and sterilization preliminary treatment for the oxygen that will get into and cultivate the chamber 2, and the oxygen that has disinfected in second pipeline 18 and the gasbag 19 is supplyed and is gone into to cultivate chamber 2 and supply the stem cell growth to use through gasbag 19 is automatic, and makes baffle 23 reciprocate through first transfer line 182, slider 20, second transfer line 22 are automatic, realizes blockking that non-sterile oxygen directly gets into and cultivates chamber 2 and pollute the stem cell.
Components, modules, mechanisms, devices and the like of the present invention, which are not described in detail, are all common standard components or components known to those skilled in the art, and the structure and the principle of the present invention can be known to those skilled in the art through technical manuals or through routine experiments.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (6)
1. A stem cell culture device is characterized by comprising a machine body (1), wherein a culture cavity (2) is formed in the machine body (1), the culture cavity (2) is communicated with the top wall of the machine body (1) through a liquid feeding channel (3), the culture cavity (2) is communicated with the bottom wall of the machine body (1) through a liquid outlet channel (4), and an electromagnetic valve (5) is arranged in the liquid outlet channel (4);
an L-shaped plate (7) and two pH value automatic adjusting box bodies (8) are fixedly connected to the top wall of the culture cavity (2), a transmission cavity (6) is enclosed by the L-shaped plate (7) and the right wall of the culture cavity (2), an oxygen supplementing pipeline is arranged in the transmission cavity (6) and connected with the right wall of the machine body (1), and one end of the oxygen supplementing pipeline extends to the outside of the machine body (1);
a liquid storage cavity (81) is formed in the pH value automatic adjusting box body (8), the lower wall of the liquid storage cavity (81) is communicated with the bottom wall of the pH value automatic adjusting box body (8) through a liquid outlet hole (82), a first transmission groove (83) and a second transmission groove (84) are formed in the side wall of the pH value automatic adjusting box body (8), and the first transmission groove (83) penetrates through the liquid outlet hole (82);
a first transmission strip (86) is arranged in the first transmission groove (83), a connecting hole (861) is formed in the first transmission strip (86), one end of the first transmission strip (86) is connected with the end of the first transmission groove (83) through a first elastic piece (862), the other end of the first transmission strip (86) is curved, and one end of the first transmission strip (86) which is curved extends to the outside of the pH value automatic adjusting box body (8);
a second transmission strip (85) is arranged in the second transmission groove (84), one end of the second transmission strip (85) is provided with a first transmission block (851), the first transmission block (851) is hemispherical, the other end of the second transmission strip (85) is provided with a pressing strip (852), one end of the second transmission strip (85) is provided with a pressing strip (852), the end part of the second transmission groove (84) is connected with one end part of the second elastic part (853), a rubber head dropper (87) is further arranged in the pH value automatic adjusting box body (8), a rubber cap (871) of the rubber head dropper (87) extends into the second transmission groove (84), the side wall of the rubber cap (871) is abutted against the end part of the second transmission groove (84), and a liquid outlet (872) of the rubber head dropper (87) extends to the bottom part of the pH value automatic adjusting box body (8);
the culture chamber (2) is internally provided with a bearing plate (9), the bearing plate (9) is provided with two transmission mechanisms, each transmission mechanism comprises a motor (10), a rotating disc (12), a first transmission plate (13) and a second transmission plate (14), the motors (10) are fixedly connected to the bearing plate (9), the rotating discs (12) are fixedly connected to a power output shaft (11) of the motors (10), the first transmission plates (13) and the second transmission plates (14) are respectively fixedly connected to the top of the rotating discs (12), the first transmission plates (13) and the second transmission plates (14) are abutted, one side wall of each first transmission plate (13) is a first inclined plane (131), one side wall of each second transmission plate (14) is a second inclined plane (141), the other side wall of each second transmission plate (14) is a third inclined plane (142), the top of each second transmission plate (14) is provided with more than two notches (143), and the rear wall of the culture chamber (2) is slidably connected with two sliding mechanisms, slide mechanism includes slide (15) and third elastic component (16), and slide (15) upper end is passed through third elastic component (16) and is connected with cultivation chamber (2) roof, and slide (15) lateral wall rigid coupling has second transmission piece (151), and second transmission piece (151) are hemispherical, and slide (15) bottom is equipped with gyro wheel (152).
2. The stem cell culture device according to claim 1, wherein the oxygen supply pipeline comprises a first pipeline (17) and a second pipeline (18), the first pipeline (17) is fixedly connected to the right wall of the machine body (1), a fourth inclined plane (173) is arranged at the left end of the first pipeline (17), the right end of the second pipeline (18) extends to the outside of the machine body (1), an ultraviolet disinfection lamp (171) is fixedly connected to the inner wall of the first pipeline (17), a rotating shaft (181) is erected on the transmission cavity (6), the second pipeline (18) is rotatably connected with the rotating shaft (181), an air bag (19) is fixedly connected at the left end of the second pipeline (18), the bottom wall of the air bag (19) is fixedly connected to the top of the horizontal section of the L-shaped plate (7), a fifth inclined plane (183) is arranged at the right end of the second pipeline (18), and the fourth inclined plane (173) and the right end of the fifth inclined plane (183) are abutted.
3. The stem cell culture device according to claim 2, wherein a rectangular loop (21) is fixedly connected to the rear wall of the transmission cavity (6), a sliding block (20) is slidably connected to the inner wall of the rectangular loop (21), a first transmission rod (182) and a second transmission rod (22) are rotatably connected to the sliding block (20), the lower end of the first transmission rod (182) is hinged to the top of the second pipeline (18), a baffle (23) is rotatably connected to the lower end of the second transmission rod (22), and the lower end of the baffle (23) extends into the first pipeline (17).
4. The stem cell culture device according to claim 3, wherein a processing module is arranged in the body (1), a pH value measuring instrument (25) is arranged at the bottom of the culture cavity (2), an oxygen concentration sensor (26) is fixedly connected to the top of the culture cavity (2), and the motor (10), the electromagnetic valve (5), the pH value measuring instrument (25) and the oxygen concentration sensor (26) are respectively and electrically connected with the processing module.
5. The stem cell culture device according to claim 4, wherein the top of the body (1) is provided with a sealing cover (24) which can be opened and closed, and the sealing cover (24) is covered on the upper end of the liquid feeding channel (3).
6. A method for culturing stem cells using the stem cell culture apparatus according to any one of claims 1 to 5, comprising the steps of:
the method comprises the following steps: adding stem cells and culture medium from the liquid adding channel (3) into the culture cavity (2);
step two: stirring the stem cells and the culture medium in the culture cavity (2) to form a mixed solution;
step three: the pH value of the mixed solution is adjusted through the two automatic pH value adjusting box bodies (8);
step four: oxygen is blown into the oxygen supplementing pipeline through an external oxygen generating device, so that a proper amount of oxygen is provided for stem cell culture in the culture cavity (2).
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| CN202111430491.XA CN114317228B (en) | 2021-11-29 | 2021-11-29 | Stem cell culture device and culture method thereof |
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| CN202111430491.XA CN114317228B (en) | 2021-11-29 | 2021-11-29 | Stem cell culture device and culture method thereof |
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| CN114317228B true CN114317228B (en) | 2022-08-19 |
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| US8026096B1 (en) * | 1998-10-08 | 2011-09-27 | Protein Sciences Corporation | In vivo active erythropoietin produced in insect cells |
| CN2661696Y (en) * | 2003-06-13 | 2004-12-08 | 上海国强生化工程装备有限公司 | Shaking table possessing real-time detection, pH control and feeding functions |
| CN101565673B (en) * | 2009-06-02 | 2011-12-21 | 扬州大学 | Intelligent bionic rumen fermenter |
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