CN115337889A

CN115337889A - Preparation process and device of blastocyst culture solution for embryo chromosome detection

Info

Publication number: CN115337889A
Application number: CN202211086831.6A
Authority: CN
Inventors: 李艳如; 张金涛; 张攀飞; 沈旋
Original assignee: Henan Provincial Peoples Hospital
Current assignee: Henan Provincial Peoples Hospital
Priority date: 2022-09-07
Filing date: 2022-09-07
Publication date: 2022-11-15
Anticipated expiration: 2042-09-07
Also published as: CN115337889B

Abstract

The invention discloses a preparation process of a blastocyst culture solution for embryo chromosome detection, belonging to the technical field of preparation of blastocyst culture solutions, and comprising the following specific steps of: injecting water into the device, placing heating equipment for heating the water at the bottom of the device, and steaming the water in the device at high temperature by the heating equipment to gasify the water into water drops; the water drops rise to the top of the device to be collected, the water drops are continuously collected and intensively dripped into a reaction kettle in the middle of the device, when the water amount in the reaction kettle reaches the standard, the reaction kettle falls, the heating equipment is separated from the device, and the preparation of the aqueous solution is stopped; step three: mixing salt, energy substances, amino acids, vitamins, proteins, etc. at a certain proportion to form liquid nutrient substance; stirring the aqueous solution in the reaction kettle to cool the aqueous solution, and adding nutrient substances according to the density of each area of the solution in the reaction kettle; the invention can automatically disinfect and sterilize water, and the disinfected and sterilized water is fully mixed with nutrient substances.

Description

Preparation process and device of blastocyst culture solution for embryo chromosome detection

Technical Field

The invention relates to the technical field of preparation of a blastocyst culture solution, in particular to a preparation process and a preparation device of a blastocyst culture solution for embryo chromosome detection.

Background

The blastocyst culture solution mainly provides nutrition and living environment for the blastocyst, the quality of the culture solution directly determines the quality of the blastocyst, and the culture solution mainly comprises water, inorganic ions, energy substrates, amino acids, vitamins, proteins and other components. Each component plays an important role in the growth and development of embryos; wherein the water accounts for 99% of the total composition of the culture solution. The quality requirement on water in the process of preparing the culture solution is very strict, organic matters, particles, bacteria, fungi and other microorganisms in the culture solution are removed, and the substances are prevented from damaging embryos, so that the development potential of the embryos is influenced.

In the traditional blastocyst culture solution preparation process, various substances required by the culture solution are directly mixed with water to obtain a finished culture solution, but the finished culture solution is influenced by the external environment in the storage and transportation processes, is easy to deteriorate and cause precipitation and layering, and can also cause the pollution of the culture solution due to poor sealing, so that the on-site preparation of the culture solution is extremely important when the blastocyst is detected with strict environmental requirements;

the on-site preparation of the culture solution is a culture solution for directly mixing sterilized water and various nutrients required by the blastocysts to form the blastocysts, but in the actual use process, the pre-prepared sterilized water has a risk of pollution in the storage or transportation process, the polluted water is used for detecting embryo chromosomes, so that misjudgment of detection personnel is easily caused, normal chromosomes are marked as chromosomes with defects at a low rate, the chromosomes with the defects are used as normal chromosomes for culture, and excessive useless culture is caused.

Disclosure of Invention

The technical problem of the invention is to provide a preparation process and a device of a blastocyst culture solution for embryo chromosome detection, which can sterilize water on site and can fully mix nutrient substances with the sterilized water.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation process of a blastula culture solution for embryo chromosome detection is suitable for a preparation device of the blastula culture solution for embryo chromosome detection, and comprises the following specific steps:

the method comprises the following steps: injecting water into the device, placing heating equipment for heating the water at the bottom of the device, and steaming the water in the device at high temperature by the heating equipment to gasify the water into water drops;

step two: the water drops rise to the top of the device to be collected, the water drops are continuously collected and intensively dripped into a reaction kettle in the middle of the device, when the water amount in the reaction kettle reaches the standard, the reaction kettle falls, the heating equipment is separated from the device, and the preparation of the aqueous solution is stopped;

step three: mixing salt, energy substances, amino acids, vitamins, proteins, etc. at a certain proportion to form liquid nutrient substance;

step four: stirring the aqueous solution in the reaction kettle to cool the aqueous solution, and adding nutrient substances according to the density of each area of the solution in the reaction kettle until the concentration of the solution in the reaction kettle reaches the standard;

step five: stopping filling the nutrient substances into the reaction kettle, and leading out the solution in the reaction kettle;

the liquid guide device comprises a container, wherein an aggregation hopper fixedly connected with an inner groove is arranged at the top of the container, a reaction kettle positioned in the container is arranged below the aggregation hopper, a liquid guide pipe in sliding connection with the container is fixedly connected to the bottom of the reaction kettle, heating equipment is fixedly connected to the bottom of the liquid guide pipe through a support, a hydraulic cylinder used for pushing a liquid pipe upwards and a limiting mechanism used for increasing the descending resistance of the liquid guide pipe are fixedly connected to the bottom of the container, a transmission shaft used for guiding liquid is coaxially arranged in the reaction kettle, a driving mechanism used for driving the transmission shaft to rotate and reciprocate up and down is installed at the top of the aggregation hopper, stirring plates which are arranged in a circumferential array and are arranged obliquely are fixedly connected to the side walls of the transmission shaft, a plurality of liquid outlet pipes which are arranged in a transverse array along the stirring plates are slidably connected to the stirring plates, slide blocks are slidably connected to the liquid outlet pipes, fixing pieces which are slidably connected to the slide blocks are fixedly connected to the inside the stirring plates, return springs are fixedly connected between the fixing pieces and the slide blocks, and liquid outlet holes are formed in the side walls of the slide blocks close to the motion direction of the stirring plates;

as a further scheme of the invention, the limiting mechanism comprises limiting lugs which are connected with the container in a sliding manner and are arranged in a circumferential array manner, the back of each limiting lug is fixedly connected with a limiting rod which is connected with the container in a sliding manner, a limiting spring which is positioned between each limiting lug and the container is sleeved on the outer wall of each limiting rod, and a limiting groove which is matched with each limiting lug is formed in the outer wall of the liquid guide tube;

as a further scheme of the invention, the driving mechanism comprises a driving motor, an output shaft of the driving motor is fixedly connected with a driving gear, the driving gear is meshed with a transmission gear fixedly connected with a transmission shaft, the bottom of the driving gear is fixedly connected with a driving worm, the driving worm is meshed with a lifting gear, the side wall of the transmission shaft is rotatably connected with a reciprocating worm, the reciprocating worm is meshed with a reciprocating gear, the reciprocating gear and the lifting gear are both fixedly connected with driving rods, and a connecting rod is rotatably connected between the driving rods;

as a further scheme of the invention, the transmission shaft comprises an upper shaft, a control switch and a lower shaft, the control switch comprises a shell fixedly connected with the bottom of the upper shaft, the top of the shell is provided with a conical cavity and a cylindrical cavity, a control spring is fixedly connected in the conical cavity, the end part of the control spring is fixedly connected with a closing plate of which the bottom extends to the cylindrical cavity, a control column fixedly connected with the lower shaft is sleeved in the cylindrical cavity, the outer wall of the control column is provided with a spiral guide groove, the inner wall of the cylindrical cavity is fixedly connected with a guide nail, and a press-fit spring is fixedly connected between the control column and the shell;

as a further scheme of the invention, the outer wall of the transmission shaft is also fixedly connected with a mixing rod, the end part of the mixing rod is fixedly connected with a self-rotating gear, the inner wall of the reaction kettle is connected with a self-rotating gear ring which slides along the axis direction in a sliding way through a sliding chute, and the self-rotating gear ring is engaged with the self-transmitting gear and is positioned above the self-transmitting gear;

as a further scheme of the invention, the liquid outlet pipes are at least arranged in two rows, and the distance between the liquid outlet pipes is gradually reduced from the center to the periphery;

as a further scheme of the invention, a buffer spring is fixedly connected between the bottom of the reaction kettle and the container.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, when the gravity of the sterilized water in the reaction kettle reaches the standard, the gravity of the reaction kettle and the water overcomes the resistance of the limiting mechanism and falls down, the heating equipment is separated from the container, and the water is continuously prepared until the water solution is prepared, and the water in the blastocyst culture solution is sterilized by a field cooking mode, so that the water in the culture solution is prevented from being transported or polluted in the preparation process, and the water cannot be used.

2. According to the invention, the driving mechanism drives the transmission shaft to rotate and move up and down in a reciprocating manner, the transmission shaft drives the stirring plate to rotate and move up and down, so that the aqueous solution in the reaction kettle can be stirred, the aqueous solution can be rapidly cooled, the time for preparing the culture solution is shortened, meanwhile, when the stirring plate rotates, the sliding block is controlled to move along with the increase of the concentration of the culture solution in the reaction kettle, the liquid outlet is gradually closed, the automatic culture solution preparation is realized, and the mixed nutrient substance is automatically stopped from being added after the concentration of the culture solution reaches the standard.

3. According to the invention, the liquid outlet pipes are arranged in a linear array along the stirring plates, the stirring plates are arranged in a circumferential array, the number of liquid outlet points of mixed nutrients is increased, so that the solution in the reaction kettle is more fully mixed, meanwhile, the distance between the liquid outlet pipes from the center to the periphery is gradually reduced, the liquid outlet amount around the reaction kettle is larger than the central position, the center of the reaction kettle, the side wall aqueous solution and the mixed nutrients are more fully mixed, and the liquid outlet pipes can vertically reciprocate while rotating, so that the moving tracks of the liquid outlet pipes are in a circumferential and wavy shape, the aqueous solution in the gaps between the upper layer of stirring plate and the lower layer of stirring plate can be mixed with the culture solution, the full degree of mixing is improved, and the mixing efficiency is also improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of the overall structure of the present invention;

FIG. 2 is a schematic view of the general cross-sectional structure of the present invention;

FIG. 3 is a schematic view of a connection structure between a driving structure and a transmission shaft according to the present invention;

FIG. 4 is an enlarged view of the structure at A in FIG. 3 according to the present invention;

FIG. 5 is a schematic view of the connection structure of the stirring plate and the transmission shaft according to the present invention;

FIG. 6 is an enlarged view of the structure at B in FIG. 5 according to the present invention;

FIG. 7 is an enlarged view of the structure of FIG. 5 at C;

FIG. 8 is a schematic view of a catheter-connection structure according to the present invention;

FIG. 9 is an enlarged view of the structure of FIG. 8 at D according to the present invention;

FIG. 10 is a schematic view of the connection structure of the drain pipes according to the present invention;

FIG. 11 is an enlarged view of E of FIG. 10 according to the present invention;

FIG. 12 is a schematic view of a manufacturing process of the present invention;

in the drawings, the components represented by the respective reference numerals are listed below:

1. a container; 11. a gathering hopper; 12. a heating device; 13. a hydraulic cylinder; 2. a reaction kettle; 21. a catheter; 31. a limiting bump; 32. a limiting rod; 33. a limiting spring; 34. a limiting groove; 4. a drive shaft; 41. a housing; 42. a conical cavity; 43. a columnar cavity; 44. a control spring; 45. a closing plate; 46. a control column; 47. a guide groove; 48. a guide pin; 49. pressing the spring; 51. a drive motor; 52. a drive gear; 53. a transmission gear; 54. a drive worm; 55. a lifting gear; 56. a drive rod; 57. a connecting rod; 58. a reciprocating gear; 59. a reciprocating worm; 6. a stirring plate; 61. a liquid outlet pipe; 62. a slider; 63. a fixing member; 64. a return spring; 65. a liquid outlet; 7. a mixing stick; 71. a rotation gear; 72. a self-rotating gear ring; 8. a buffer spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-12, the present invention provides a technical solution: the device comprises a container 1, the top of the container 1 is a gathering hopper 11 fixedly connected with an inner groove, a reaction kettle 2 positioned in the container 1 is arranged below the gathering hopper 11, the bottom of the reaction kettle 2 is fixedly connected with a liquid guide pipe 21 in sliding connection with the container 1, the bottom of the liquid guide pipe 21 is fixedly connected with a heating device 12 through a support, the bottom of the container 1 is fixedly connected with a hydraulic cylinder 13 used for pushing the liquid guide pipe 21 upwards and a limiting mechanism used for increasing descending obstruction of the liquid guide pipe 21, a transmission shaft 4 used for guiding liquid is coaxially arranged in the reaction kettle 2, the top of the gathering hopper 11 is provided with a driving mechanism used for driving the transmission shaft 4 to rotate and reciprocate up and down, the side wall of the transmission shaft 4 is fixedly connected with a stirring plate 6 which is arranged in a circumferential array and inclined manner, the stirring plate 6 is slidably connected with a plurality of liquid outlet pipes 61 arranged in a transverse array manner along the stirring plate 6, a sliding block 62 is slidably connected in the liquid outlet pipe 61, a fixing member 63 slidably connected with the sliding block 62 is fixedly connected in the stirring plate 6, a return spring 64 is fixedly connected between the fixing member 63 and the sliding block 62, and a liquid outlet 65 is arranged close to the side wall of the stirring plate 6 in the moving direction;

when the device works, various substances (inorganic ions, energy substrates, amino acids, vitamins, proteins and other components) required by a blastocyst culture solution are mixed and stored in a special tank, nutrient substances can be adjusted according to the type of blastocysts, the tank for storing the mixed nutrient substances is communicated with the transmission shaft 4, water is injected into the container 1, the movable end of the hydraulic cylinder 13 rises to push the liquid guide pipe 21 upwards to the movable end of the hydraulic cylinder 13 to be only contacted with the bottom of the liquid guide pipe 21, the liquid guide pipe 21 pushes the reaction kettle 2 upwards, meanwhile, the liquid guide pipe 21 drives the heating device 12 to rise until the liquid guide pipe 21 is attached to the bottom of the container 1, the limiting mechanism limits the reaction kettle 2 through the liquid guide pipe 21, so that the reaction kettle 2 keeps the height, the movable end of the hydraulic cylinder 13 is reset, the heating equipment 12 heats the water in the container 1, the heated water is boiled and gasified to steam and rises, the steam is contacted with the gathering hopper 11 and then is gathered to form water drops again, the water drops are dripped into the reaction kettle 2 along the gathering hopper 11 under the action of gravity, the water in the reaction kettle 2 is sterilized at high temperature to reach the standard of preparing culture solution, the weight of the reaction kettle 2 is continuously increased along with the continuous increase of the water in the reaction kettle 2, the gravity of the reaction kettle 2 and the water overcomes the obstruction of the limiting mechanism and falls, the reaction kettle 2 drives the heating equipment 12 to fall through the liquid guide pipe 21, and the heating equipment 12 is separated from the bottom of the container 1 and stops heating the water in the container 1; the heating device 12 is powered off, the aqueous solution is static after being static for a period of time, which is beneficial to cooling the aqueous solution, and prevents substances such as protein in the mixed nutrient substances from deteriorating due to the high-temperature aqueous solution, the driving mechanism is started, the driving mechanism drives the transmission shaft 4 to rotate and reciprocate up and down, and the transmission shaft 4 drives the stirring plate 6 to rotate and reciprocate up and down, so that the speed of water cooling in the reaction kettle 2 can be accelerated, and the preparation speed is accelerated;

the culture solution enters the stirring plate 6 along the transmission shaft 4, because the slide block 62 is in a dislocation state with the liquid outlet 65 at the end of the liquid outlet pipe 61 and the liquid guide pipe 21 under the elastic force of the return spring 64, the reaction kettle 2 is in a dislocation state with the liquid outlet 65 at the end of the slide block 62, the mixed nutrient substance is in an open liquid outlet pipe 61, the mixed nutrient substance enters the slide block 62 from the stirring plate 6 and enters the reaction kettle 2 through the liquid outlet 65 to be mixed with water, the concentration of the solution in the reaction kettle 2 is gradually increased along with the increase of the mixed nutrient substance, the resistance of the solution to the slide block 62 is also increased along with the increase of the amount of the mixed nutrient substance entering the reaction kettle 2, the liquid outlet 65 gradually slides towards the liquid outlet pipe 61 in the stirring plate 6, until the concentration of the solution in the reaction kettle 2 reaches the standard of use of a blastocyst, the liquid outlet 65 is overlapped with the liquid outlet pipe 61, the mixed nutrient substance stops entering the reaction kettle 2, at the moment, the preparation of the culture solution is completed, and the culture solution is led out of the reaction kettle 2 through the liquid guide pipe 21,

in the process, when the gravity of the sterilized water in the reaction kettle 2 reaches the standard, the gravity of the reaction kettle 2 and the water overcomes the resistance of the limiting mechanism and falls, so that the heating device 12 is separated from the container 1 and stops continuing to prepare the aqueous solution, and the water in the blastocyst culture solution is sterilized in a field cooking mode, so that the water in the culture solution is prevented from being transported or polluted in the preparation process and cannot be used;

the driving mechanism drives the transmission shaft 4 to rotate and move up and down in a reciprocating manner, the transmission shaft 4 drives the stirring plate 6 to rotate and move up and down, so that the aqueous solution in the reaction kettle 2 can be stirred, the aqueous solution can be rapidly cooled, the culture solution preparation time is shortened, meanwhile, when the stirring plate 6 rotates, the sliding block 62 is controlled to move along with the increase of the concentration of the culture solution in the reaction kettle 2, the liquid outlet 65 is gradually closed, the automatic culture solution preparation is realized, and the mixed nutrient substance is automatically stopped from being added after the concentration of the culture solution reaches the standard;

because the liquid outlet pipes 61 are arranged along the stirring plates 6 in a linear array manner, and the stirring plates 6 are arranged in a circumferential array manner, the number of liquid outlet points of mixed nutrient substances is increased, so that the solution in the reaction kettle 2 is more fully mixed, meanwhile, the distance between the liquid outlet pipes 61 from the center to the periphery is gradually reduced, the liquid outlet amount around the reaction kettle 2 is larger than the central position, so that the water solution in the center and the side wall of the reaction kettle 2 and the mixed nutrient substances are more fully mixed, and the liquid outlet pipes 61 can move up and down in a reciprocating manner while rotating, so that the moving tracks of the liquid outlet pipes 61 are in a circular and wavy manner, so that the water solution in the gaps between the upper layer of stirring plate 6 and the lower layer of stirring plate 6 can be mixed with the culture solution, the full degree of mixing is improved, and the mixing efficiency is also improved;

as a further scheme of the invention, the limiting mechanism comprises limiting lugs 31 which are connected with the container 1 in a sliding manner and are arranged in a circumferential array manner, the back of each limiting lug 31 is fixedly connected with a limiting rod 32 which is connected with the container 1 in a sliding manner, a limiting spring 33 which is positioned between the limiting lug 31 and the container 1 is sleeved on the outer wall of each limiting rod 32, and a limiting groove 34 which is matched with the limiting lugs 31 is formed in the outer wall of the liquid guide tube 21;

when the hydraulic cylinder 13 pushes the liquid guide tube 21 to ascend, and the limiting groove 34 and the limiting lug 31 are at the same height, the limiting lug 31 is bounced into the limiting groove 34 under the action of the limiting spring 33, so that the liquid guide tube 21 is limited, and the reaction kettle 2 is kept still; when the amount of the aqueous solution reaches the standard, the reaction kettle 2 extrudes the limiting bump 31 through the liquid guide pipe 21, the limiting bump 31 is separated from the limiting groove 34, the liquid guide pipe 21 descends, the heating device 12 at the bottom of the liquid guide pipe 21 is separated from the bottom of the container 1, so that when the amount of the aqueous solution reaches the required amount, the heating of the container 1 is automatically stopped, the preparation of the aqueous solution is stopped, and the movable end of the hydraulic cylinder 13 is in contact with the liquid guide pipe 21 again;

as a further scheme of the invention, the driving mechanism comprises a driving motor 51, an output shaft of the driving motor 51 is fixedly connected with a driving gear 52, the driving gear 52 is meshed with a transmission gear 53 fixedly connected with the transmission shaft 4, the bottom of the driving gear 52 is fixedly connected with a driving worm 54, the driving worm 54 is meshed with a lifting gear 55, the side wall of the transmission shaft 4 is rotatably connected with a reciprocating worm 59, the reciprocating worm 59 is meshed with a reciprocating gear 58, the reciprocating gear 58 and the lifting gear 55 are both fixedly connected with a driving rod 56, and a connecting rod 57 is rotatably connected between the driving rods 56;

after the water solution amount in the reaction kettle 2 reaches the standard, the driving motor 51 drives the driving gear 52 to rotate, the driving gear 52 drives the transmission shaft 4 to rotate through the transmission gear 53 meshed with the driving gear 52, so that the cooling of the water solution and the mixing of mixed nutrient substances are accelerated; meanwhile, the driving gear 52 drives the driving worm 54 which is coaxially arranged to rotate, the driving worm 54 drives the lifting gear 55 to continuously rotate, the lifting gear 55 drives the driving rod 56 which is fixedly connected with the lifting gear 55 to rotate, the driving rod 56 drives one end of the driving rod 56 which is fixedly connected with the reciprocating gear 58 to reciprocate up and down through the connecting rod 57, so that the reciprocating gear 58 rotates in a reciprocating manner, the reciprocating gear 58 drives the transmission shaft 4 to reciprocate up and down through the reciprocating worm 59, so that the transmission shaft 4 and the stirring plate 6 reciprocate up and down, and the cooling speed of the aqueous solution and the mixing speed of the mixed nutrient substances are further increased;

as a further scheme of the present invention, the transmission shaft 4 includes an upper shaft, a control switch, and a lower shaft, the control switch includes a housing 41 fixedly connected to the bottom of the upper shaft, the top of the housing 41 is provided with a conical cavity 42 and a cylindrical cavity 43, the conical cavity 42 is internally and fixedly connected with a control spring 44, the end of the control spring 44 is fixedly connected with a closing plate 45 whose bottom extends to the cylindrical cavity 43, the cylindrical cavity 43 is internally sleeved with a control column 46 fixedly connected to the lower shaft, the outer wall of the control column is provided with a spiral guide groove 47, the inner wall of the cylindrical cavity 43 is fixedly connected with a guide nail 48, and a press spring 49 is fixedly connected between the control column 46 and the housing 41;

when the device works, the upper shaft of the transmission shaft 4 rotates, so that the shell 41 rotates, the shell 41 drives the guide nail 48 to rotate, the guide nail 48 rotates along the guide groove 47 due to the speed difference between the upper shaft and the lower shaft, the control column 46 rises and pushes up the closing plate 45, the closing plate 45 moves upwards, so that the bottom of the conical cavity 42 is opened, mixed nutrient substances can enter the lower shaft from the upper shaft and then enter the stirring plate 6, when the transmission shaft 4 stops, the control column 46 is pushed down by the elasticity of the pressing spring 49, so that the upward pushing force of the closing plate 45 is lost, the closing plate 45 is pushed to the bottom of the conical cavity 42 by the control spring 44 and is blocked, and the situation that the mixed nutrient substances enter the reaction kettle 2 to cause high-temperature deterioration of the mixed nutrient substances during preparation of an aqueous solution can be avoided;

as a further scheme of the invention, the outer wall of the transmission shaft 4 is also fixedly connected with a mixing rod 7, the end part of the mixing rod 7 is fixedly connected with a self-rotating gear 71, the inner wall of the reaction kettle 2 is connected with a self-rotating gear ring 72 which slides along the axial direction in a sliding way through a sliding chute, and the self-rotating gear ring 72 is engaged with the self-transmitting gear and is positioned above the self-transmitting gear;

when the transmission shaft 4 rotates, the mixing rod 7 rotates around the transmission shaft 4, so that mixed nutrient substances flowing out of the liquid outlet pipe 61 can be further mixed with aqueous solution, meanwhile, the mixing rod 7 drives the rotation gear 71 to move, the rotation gear 71 drives the mixing rod 7 to rotate under the action of the rotation gear ring 72, so that the mixing rod 7 rotates, the further mixing speed of the mixed nutrient substances flowing out of the liquid outlet pipe 61 and the aqueous solution is further increased, the preparation time of the culture solution is reduced, in addition, the rotation gear ring 72 and the reaction kettle 2 are arranged in a sliding mode, the vertical reciprocating movement of the reaction kettle 2 and the rotation gear 71 is not influenced, the rotation gear ring 72 can be always meshed with the rotation gear 71 under the action of gravity, when the position difference is generated between the rotation gear ring 72 and the reaction kettle 2, the inner wall of the reaction kettle 2 can be cleaned, the culture solution is prevented from remaining on the inner wall of the reaction kettle 2, and the remaining culture solution is stored and deteriorated for a long time, so that the preparation of the subsequent culture solution is influenced;

as a further proposal of the invention, at least two rows of the liquid outlet pipes 61 are arranged, and the distance between the liquid outlet pipes 61 is gradually reduced from the center to the periphery;

when the aqueous solution and the mixed nutrient substance are mixed, the mixed nutrient substance can be added into the reaction kettle 2 from different positions by the liquid outlet pipes 61 in multiple rows, so that the condition of different concentrations of all regions is reduced, and meanwhile, the mixed nutrient substance can be supplemented to a single position according to the resistance of each liquid outlet pipe 61, so that the condition of different concentrations of all regions is further improved; the distance between the liquid outlet pipes 61 is gradually reduced from the center to the periphery, so that the speed of supplementing mixed nutrient substances on the outer wall of the reaction kettle 2 can be increased, and the condition that the path of the liquid outlet pipe 61 on the outer side is larger than that of the liquid outlet pipe 61 on the inner side is met;

as a further scheme of the invention, a buffer spring 8 is fixedly connected between the bottom of the reaction kettle 2 and the container 1;

when reation kettle 2 whereabouts, buffer spring 8 can play the effect of buffering, avoids catheter 21 and pneumatic cylinder 13 direct striking, with higher speed loss between them, reduces the rocking that undulant leads to reation kettle 2 interior aqueous solution simultaneously, leads to the aqueous solution spill, causes the aqueous solution loss, and then leads to the culture solution volume of final configuration preparation not enough.

A preparation process of a blastula culture solution for embryo chromosome detection is suitable for a preparation device of the blastula culture solution for embryo chromosome detection, and comprises the following specific steps:

the method comprises the following steps: injecting water into the device, placing a heating device 12 for heating the water at the bottom of the device, and steaming the water in the device at high temperature by the heating device 12 to gasify the water into water drops;

step two: the water drops rise to the top of the device to be collected, the water drops are continuously collected and are intensively dripped into the reaction kettle 2 in the middle of the device, when the water amount in the reaction kettle 2 reaches the standard, the reaction kettle 2 falls, the heating equipment 12 is separated from the device, and the preparation of the aqueous solution is stopped;

step four: stirring the aqueous solution in the reaction kettle 2 to cool the aqueous solution, and adding nutrient substances according to the density of each area of the solution in the reaction kettle 2 until the concentration of the solution in the reaction kettle 2 reaches the standard;

step five: stopping filling nutrient substances into the reaction kettle 2, and leading out the solution in the reaction kettle 2.

Claims

1. A preparation process of a blastocyst culture solution for embryo chromosome detection is suitable for a preparation device of the blastocyst culture solution for embryo chromosome detection, and is characterized in that: the preparation process of the blastula culture solution for embryo chromosome detection comprises the following specific steps:

the method comprises the following steps: injecting water into the device, placing a heating device (12) for heating the water at the bottom of the device, and steaming the water in the device by the heating device (12) at high temperature to gasify the water into water drops;

step two: the water drops rise to the top of the device to be collected, the water drops are continuously collected and dropped into the reaction kettle (2) in the middle of the device, when the water amount in the reaction kettle (2) reaches the standard, the reaction kettle (2) falls down, so that the heating equipment (12) is separated from the device, and the preparation of the aqueous solution is stopped;

step four: stirring the aqueous solution in the reaction kettle (2) to cool the aqueous solution, and adding nutrient substances according to the density of each area of the solution in the reaction kettle (2) until the concentration of the solution in the reaction kettle (2) reaches the standard;

step five: stopping filling the nutrient substances into the reaction kettle (2), and leading out the solution in the reaction kettle (2).

2. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 1, comprising a container (1), characterized in that: container (1) top is gathering fill (11) of fixedly connected with inside groove, gathering fill (11) below is provided with reation kettle (2) that are located container (1) inside, reation kettle (2) bottom fixedly connected with and container (1) sliding connection's catheter (21), catheter (21) bottom is through support fixedly connected with heating equipment (12), container (1) bottom fixedly connected with is used for deriving pneumatic cylinder (13) of liquid pipe (21) on and is used for increasing catheter (21) decline stop gear that hinders, coaxial drive shaft (4) that are used for leading liquid in reation kettle (2), gathering fill (11) top is installed and is used for driving drive shaft (4) rotation and up-and-down reciprocating motion's actuating mechanism, drive shaft (4) lateral wall fixedly connected with and be stirring board (6) that circumference array arranged and slope setting, stirring board (6) sliding connection has a plurality of discharge pipe (61) that arrange along stirring board (6) transverse array, sliding connection has liquid outlet slider (62) sliding connection slider (63) in (6), slider and slider (63) sliding connection has seted up between slider (62) and the movement direction (64) of slider (62), reset spring (64) are close to discharge board (6) and reset spring (64) are seted up to fixing piece (6) sliding connection between slider (62), and slider (6) sliding connection slider (62), and the side wall (6) of sliding connection of fixing element (6) are close to the movement of discharge pipe (6) of sliding connection (65).

3. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 2, wherein: stop gear includes with container (1) sliding connection and be spacing lug (31) that circumference array arranged, spacing lug (31) back fixedly connected with and container (1) sliding connection's gag lever post (32), gag lever post (32) outer wall cover is equipped with and is located spacing lug (31) and container (1) spacing spring (33), catheter (21) outer wall is seted up and is limited recess (34) with spacing lug (31) matched with.

4. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 3, wherein: the drive mechanism includes driving motor (51), driving motor (51) output shaft fixedly connected with drive gear (52), drive gear (52) meshing have with transmission shaft (4) fixed connection's drive gear (53), drive gear (52) bottom fixedly connected with drive worm (54), drive worm (54) meshing has lifting gear (55), transmission shaft (4) lateral wall rotates and is connected with reciprocal worm (59), reciprocal worm (59) meshing has reciprocating gear (58), reciprocating gear (58) and the equal fixedly connected with actuating lever (56) of lifting gear (55), it is connected with connecting rod (57) to rotate between actuating lever (56).

5. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 4, wherein: the transmission shaft (4) comprises an upper shaft, a control switch and a lower shaft, wherein the control switch comprises a shell (41) fixedly connected with the bottom of the upper shaft, a conical cavity (42) and a cylindrical cavity (43) are arranged at the top of the shell (41), a control spring (44) is fixedly connected in the conical cavity (42), the bottom of the end portion of the control spring (44) is fixedly connected with a closing plate (45) extending to the cylindrical cavity (43), a control column (46) fixedly connected with the lower shaft is sleeved in the cylindrical cavity (43), a spiral guide groove (47) is formed in the outer wall of the control column, a guide nail (48) is fixedly connected to the inner wall of the cylindrical cavity (43), and a press-fit spring (49) is fixedly connected between the control column (46) and the shell (41).

6. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 5, wherein: the outer wall of the transmission shaft (4) is further fixedly connected with a mixing rod (7), the end part of the mixing rod (7) is fixedly connected with a rotation gear (71), the inner wall of the reaction kettle (2) is connected with a rotation gear ring (72) which slides along the axis direction through a sliding groove in a sliding mode, and the rotation gear ring (72) is meshed with the self-transmission gear and is located above the self-transmission gear.

7. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 6, wherein: the liquid outlet pipes (61) are at least arranged in two rows, and the distance between the liquid outlet pipes (61) is gradually reduced from the center to the periphery.

8. The apparatus for preparing a blastocyst culture solution for embryo chromosome detection according to claim 7, wherein: and a buffer spring is fixedly connected between the bottom of the reaction kettle (2) and the container (1). (8).