CN116323945A - 驱动环状rna翻译的遗传元件和使用方法 - Google Patents

驱动环状rna翻译的遗传元件和使用方法 Download PDF

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CN116323945A
CN116323945A CN202180052292.1A CN202180052292A CN116323945A CN 116323945 A CN116323945 A CN 116323945A CN 202180052292 A CN202180052292 A CN 202180052292A CN 116323945 A CN116323945 A CN 116323945A
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ires
sequence
protein
nucleic acid
circular rna
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H·Y·常
C-K·陈
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Leland Stanford Junior University
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Leland Stanford Junior University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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CN202180052292.1A 2020-06-25 2021-06-25 驱动环状rna翻译的遗传元件和使用方法 Pending CN116323945A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063043964P 2020-06-25 2020-06-25
US63/043964 2020-06-25
US202163186507P 2021-05-10 2021-05-10
US63/186507 2021-05-10
PCT/US2021/039127 WO2021263124A2 (en) 2020-06-25 2021-06-25 Genetic elements driving circular rna translation and methods of use

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CN116323945A true CN116323945A (zh) 2023-06-23

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US (3) US11560567B2 (https=)
EP (1) EP4171631A4 (https=)
JP (1) JP2023532663A (https=)
KR (1) KR20230028375A (https=)
CN (1) CN116323945A (https=)
AU (1) AU2021297340A1 (https=)
CA (1) CA3180224A1 (https=)
WO (1) WO2021263124A2 (https=)

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WO2025077734A1 (en) * 2023-10-09 2025-04-17 Shanghai Circode Biomed Co., Ltd. Constructs and methods for preparing circular rnas and uses thereof

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US20240299520A1 (en) * 2021-02-09 2024-09-12 Virginia Commonwealth University Mini circular rna therapeutics and vaccines and methods of use thereof
IL312965A (en) 2021-11-24 2024-07-01 Flagship Pioneering Innovations Vi Llc Immunogenic compositions and their uses
EP4436984A1 (en) 2021-11-24 2024-10-02 Flagship Pioneering Innovations VI, LLC Coronavirus immunogen compositions and their uses
CN118401544A (zh) 2021-11-24 2024-07-26 旗舰创业创新六公司 水痘-带状疱疹病毒免疫原组合物及其用途
KR20240118174A (ko) 2021-12-17 2024-08-02 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 변성 조건 하에서의 원형 rna의 풍부화를 위한 방법
EP4453201A1 (en) 2021-12-22 2024-10-30 Flagship Pioneering Innovations VI, LLC Compositions and methods for purifying polyribonucleotides
KR20240118881A (ko) 2021-12-23 2024-08-05 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 항푸소제닉 폴리펩티드를 인코딩하는 원형 폴리리보뉴클레오티드
CN119095967A (zh) * 2022-02-24 2024-12-06 普罗拜奥根股份公司 病毒样颗粒介导的环状rna递送
EP4493197A4 (en) * 2022-03-17 2026-03-18 Univ Leland Stanford Junior COMPOSITIONS AND METHODS FOR ENHANCED PROTEIN TRANSLATION FROM RECOMBED CIRCULAR RNA
US20250320519A1 (en) 2022-05-30 2025-10-16 Shanghai Circode Biomed Co., Ltd. Synthetic circular rna compositions and methods of use thereof
JP2025519910A (ja) * 2022-06-24 2025-06-26 ザ・ブロード・インスティテュート・インコーポレイテッド キャップされた環状ｒｎａ分子を調製するための組成物及び方法
US20260002223A1 (en) * 2022-06-27 2026-01-01 Wisconsin Alumni Research Foundation Universal method for parasite and eukaryotic endosymbiont identification
KR20240003761A (ko) * 2022-06-29 2024-01-09 서울대학교산학협력단 mRNA 번역 증가용 조절 엘리먼트를 스크리닝하는 방법,상기 방법에 따른 신규 조절 엘리먼트, 및 이의 용도
WO2024010028A1 (ja) * 2022-07-06 2024-01-11 国立大学法人京都大学 環状ｒｎａ分子及びこれを用いた翻訳制御方法、翻訳活性化システム、並びに医薬組成物
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TW202434728A (zh) 2022-11-08 2024-09-01 美商旗艦先鋒創新有限責任公司 用於產生環狀多核糖核苷酸的組成物和方法
KR20250122519A (ko) 2022-12-22 2025-08-13 젠스크립트 유에스에이 인크. 자가환형화 rna의 제조 방법
WO2024151583A2 (en) 2023-01-09 2024-07-18 Flagship Pioneering Innovations Vii, Llc Vaccines and related methods
CN121057577A (zh) 2023-01-27 2025-12-02 赛欧生物医药股份有限公司 经修饰的脂质组合物及其用途
WO2024167885A1 (en) 2023-02-06 2024-08-15 Flagship Pioneering Innovations Vii, Llc Immunomodulatory compositions and related methods
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CN121358494A (zh) 2023-03-15 2026-01-16 旗舰创业创新第六有限责任公司 免疫原性组合物及其用途
WO2024220752A2 (en) 2023-04-19 2024-10-24 Sail Biomedicines, Inc. Rna therapeutic compositions
WO2024220625A1 (en) 2023-04-19 2024-10-24 Sail Biomedicines, Inc. Delivery of polynucleotides from lipid nanoparticles comprising rna and ionizable lipids
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WO2024226621A1 (en) * 2023-04-25 2024-10-31 Ginkgo Bioworks, Inc. Novel internal ribosome entry site (ires) and intron sequences
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WO2024254546A2 (en) * 2023-06-08 2024-12-12 Cornell University Virus-like particles comprising circular mrna expression systems and methods of use thereof
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WO2025034727A1 (en) * 2023-08-07 2025-02-13 Yale University Translational modulating elements for mrna molecules capped with 5'-cap analogs, mrna molecules including the same, and methods using the same
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WO2025106915A1 (en) 2023-11-17 2025-05-22 Sail Biomedicines, Inc. Circular polyribonucleotides encoding glucagon-like peptide 1 (glp-1) and uses thereof
WO2025106930A1 (en) 2023-11-17 2025-05-22 Sail Biomedicines, Inc. Circular polyribonucleotides encoding glucagon-like peptide 2 (glp-2) and uses thereof
TW202545974A (zh) 2024-01-26 2025-12-01 美商旗艦先鋒創新有限責任（Ｖｉｉ）公司 免疫受體抑制蛋白及相關方法
WO2025179198A1 (en) 2024-02-23 2025-08-28 Sail Biomedicines, Inc. Circular polyribonucleotides and unmodified linear rnas with reduced immunogenicity
US20250353881A1 (en) 2024-05-16 2025-11-20 Flagship Pioneering Innovations Vii, Llc Immunoreceptor inhibitory proteins and related methods
WO2025245111A1 (en) 2024-05-22 2025-11-27 Flagship Pioneering Innovations Vii, Llc Immunoreceptor targeting proteins and related methods
WO2025250751A1 (en) 2024-05-31 2025-12-04 Orna Therapeutics, Inc. Circular rna compositions and methods
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WO2026060273A1 (en) * 2024-09-13 2026-03-19 Shape Therapeutics Inc. 5' untranslated regions (utrs) for enhanced payload expression
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US11560567B2 (en) 2023-01-24
JP2023532663A (ja) 2023-07-31
US20230125935A1 (en) 2023-04-27
AU2021297340A1 (en) 2023-01-19
US20230407317A1 (en) 2023-12-21
EP4171631A4 (en) 2025-02-12
CA3180224A1 (en) 2021-12-30
US11685924B2 (en) 2023-06-27
WO2021263124A2 (en) 2021-12-30
US20220251578A1 (en) 2022-08-11
KR20230028375A (ko) 2023-02-28
EP4171631A2 (en) 2023-05-03

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