CN1163117C - Mutagenic maintenance method for standard female line of castor - Google Patents
Mutagenic maintenance method for standard female line of castor Download PDFInfo
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- CN1163117C CN1163117C CNB001318578A CN00131857A CN1163117C CN 1163117 C CN1163117 C CN 1163117C CN B001318578 A CNB001318578 A CN B001318578A CN 00131857 A CN00131857 A CN 00131857A CN 1163117 C CN1163117 C CN 1163117C
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Abstract
The present invention relates to a plant variety production method, namely a mutagenic maintenance method of standard female lines of castor. In the method of the present invention, castor variation is generated by nuclear radiation, a castor isofemale line with a distinguishing mark and a maintenance line thereof are obtained, and therefore, conditions are provided for the wide implementation of a castor hybridization technique.
Description
The present invention relates to a kind of plant variety production method.It is a kind of castor-oil plant isofemale line mutagenic maintenance method easy to identify.
Castor-oil plant intraspecific hybridization advantage is fairly obvious, and this point is proved by a large amount of experiments., give birth on same rhachis because female flower of castor-oil plant and male flower, it is difficult to castrate, thereby has limited the application of hybridization technique.In order to address this problem, people attempt to seek natural isofemale line (also claiming female system or male sterile line) and maintenance line thereof in the middle of castor-oil plant colony.Yet, seek the isofemale line and the maintenance line thereof that meet people's requirement, be not easy very much.Up to the present, the research of domestic this respect still is in the stage of fumbling.In recent years, along with the application of nuclear technique in agri-scientific research, people wish that the method that adopts ray to handle obtains isofemale line.,, both made to obtain isofemale line but still exist two hang-ups for castor-oil plant, the one, not easy to identify through handling the isofemale line that produces variation.The 2nd, through the isofemale line that mutagenesis produces, do not have maintenance line, thereby can't satisfy the requirement of large tracts of land breeding for its procreation.
The purpose of this invention is to provide and a kind ofly can obtain the castor-oil plant isofemale line being convenient to discern and the method for maintenance line thereof.
Above-mentioned purpose is realized by following technical scheme: adopting dosage is 20,000 human relations, dose rate be 134 human relations/minute
60C
OrRay carries out irradiation treatment to the castor-oil plant seed, in the middle of its offspring, produce a kind of male-sterility, and the isofemale line plant that living section bears leaflet at the female flower of rhachis, be called and have the castor-oil plant isofemale line that indicates leaflet, also claim standard female line of castor, with this mark female be plant and other normal plant mating, can obtain the maintenance line that this marks female system in its offspring's the normal strain.
The said female system of mark that produces in the castor-oil plant seed offspring of irradiation treatment refers to following several mode:
A, after the sowing of the castor-oil plant seed of irradiation treatment grows, produce again through selfing and to make seed, again sowing produce two generation plant, therefrom can obtain to mark female system by observing.
B, will be female by the mark that preceding method obtains be the seed that plant and other normal strain castor-oil plant mating are produced, through the seed that sowing, selfing produce again, after planting can isolate mark more female is plant.
C, will be by the female system of the mark that preceding method obtains and its maintenance line mating, can isolate the female system of mark.
Producing this mode of marking the maintenance line of female system among the offspring of female system of said mark and normal strain mating has:
The normal strain of A, the female system of mark and same district is carried out sisters and is handed over the next generation who is produced to be maintenance line.
The castor-oil plant mating of B, the female system of mark and other kinds, the next generation who is produced is maintenance line.
The seed that C, the female system of mark and other normal strain mating are produced is sowed again and through the seed that selfing produced, sowing can be isolated maintenance line again.
D, will mark female system and the maintenance line mating that obtains as stated above, its separable maintenance line that goes out of future generation.
Introduce two kinds of embodiment below in conjunction with accompanying drawing:
Fig. 1 is normal castor-oil plant aspect graph in squaring period.
Fig. 2 is that marking female is castor-oil plant aspect graph in squaring period.
Fig. 3 is normal castor-oil plant fruiting period aspect graph.
Fig. 4 is that marking female is castor-oil plant fruiting period aspect graph.
Fig. 5 is the gene map of first kind of embodiment.
Fig. 6 and Fig. 7 are the gene maps of second kind of embodiment.
Among the figure as seen: rhachis 1, the sign leaf 2.
First kind of embodiment:
Select a castor-oil plant kind for use, its seed is carried out
60C
OrRay is handled, and dosage is 20,000 human relations, dose rate be 134 human relations/minute.After seeds treated is sowed, produce normal strain, selfing is carried out in this normal strain, produced two generation seed sow again, when treating that plant grows main rhachis, promptly can be observed on the rhachis 1 of part plant, leaflet, claimed sign leaf 2, this part plant is exactly the isofemale line plant, because this plant has discernible sign leaf, so claim mark female system.
Marking female is that plant is accepted normal strain pollen, for example with the same district in normal strain carry out sisters' friendship, perhaps carrying out test cross with other selected kinds can.Produce next on behalf of normal strain, claim F1.This F1 is exactly the maintenance line of the female system of mark, is mating with this part maintenance line with marking female, and it is plant that its next generation can obtain only about half of mark female, with only about half of maintenance line plant.
Second kind of embodiment:
Because directly the grain weight of handling is few, the cost height, thereby in production practices, often adopt backcross method, be about to F
1Selfing obtains the seed full dose and sows, living plant F
2Isolating mark about 1/4th female is plant.With F
2In female system of mark and F
1Backcross, will produce about 1/2nd mark female is plant, and all the other 1/2nd plant are its maintenance line.
The reason that produces above-mentioned phenomenon is: marking female system has female gene (aa) and marker trait gene (bb), and normal strain has male flower development gene (AA) and no marks character gene (BB).Female sex and the marker trait of marking female system are to be subjected to two pairs of recessive gene (aabb) controls of closely interlocking.And have the male flower growth and the no marks proterties of normal strain are to be subjected to two pairs of dominance nuclear gene (AABB) controls of closely interlocking in addition, and it is hybridized as Fig. 5, Fig. 6, shown in Figure 7.
As seen from Figure 5, the mark that produces through mutagenesis is female to be that plant is handed over normal strain sisters or test cross produces Offspring F1, and its female gene aa and marker trait gene bb are recessive, so plant is normal strain.This normal strain is the maintenance line of the female system of mark.F1 and the female system of mark are intersected, and can obtain approximately half mark female is plant and second half maintenance line plant.Go on according to this, can obtain female system of enough marks and maintenance line.
As shown in Figure 6, F
1Carry out selfing, gained seed full dose is sowed, the gained plant has produced separation, and wherein normal plant accounts for 1 part fully, and the maintenance line plant puts two parts approximately, and marking female is that plant accounts for 1 part.
As seen from Figure 7, with above-mentioned F
2In mark female be that plant and F1 backcross, its offspring D
1Be separated into the maintenance line AaBb identical and mark the female aabb of being two parts, respectively account for half approximately with F1.
According to above-mentioned analysis as can be known, handle to produce the female system of mark that variation is obtained through ray, stabilization characteristics of genetics not only, and be easy to keep, like this, just for castor-oil plant isofemale line seed breed and hybrid seeding provides assurance.
When the large tracts of land production of hybrid seeds, the seed that method obtained shown in Fig. 5,6,7 can be doubled to sow, treat that the plant marker trait occurs after, remove normal plant, remaining whole be the isofemale line plant, can hybridize with other male parents according to plan.
Claims (3)
1, a kind of castor-oil plant isofemale line mutagenic maintenance method easy to identify is characterized in that: adopting dosage is 20,000 human relations, dose rate be 134 human relations/minute
60C
OrRay carries out the width of cloth to the castor-oil plant seed and penetrates processing, in the middle of its offspring, produce a kind of male-sterility, and the isofemale line plant that living section bears leaflet at the female flower of rhachis (1), be called the castor-oil plant isofemale line that has sign leaflet (2), also claim standard female line of castor, with this mark female be plant and other normal plant mating, can obtain the maintenance line that this marks female system in its offspring's the normal strain.
2, castor-oil plant isofemale line mutagenic maintenance method easy to identify according to claim 1 is characterized in that: said penetrating through the width of cloth produces the female system of mark among the castor-oil plant seed offspring of processing, refer to following several mode:
A, after the castor-oil plant seed sowing that the width of cloth is penetrated processing grows, produce again through selfing and to make seed, again sowing produce two generation plant, therefrom can obtain to mark female system by observing,
B, will be female by the mark that preceding method obtains be the seed that plant and other normal strain castor-oil plant mating are produced, through the seed that sowing, selfing produce again, after planting can isolate mark more female is plant,
C, will be by the female system of the mark that preceding method obtains and its maintenance line mating, can isolate the female system of mark.
3, castor-oil plant isofemale line mutagenic maintenance method easy to identify according to claim 1 is characterized in that: producing this mode of marking the maintenance line of female system among the offspring of female system of said mark and normal strain mating has:
The normal strain of A, the female system of mark and same district is carried out sisters and is handed over the next generation who is produced to be maintenance line,
The castor-oil plant mating of B, the female system of mark and other kinds, the next generation who is produced is maintenance line,
The seed that C, the female system of mark and other normal strain mating are produced is sowed again and through the seed that selfing produced, sowing can be isolated maintenance line again,
D, will mark female system and the maintenance line mating that obtains as stated above, its separable maintenance line that goes out of future generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001318578A CN1163117C (en) | 2000-11-08 | 2000-11-08 | Mutagenic maintenance method for standard female line of castor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001318578A CN1163117C (en) | 2000-11-08 | 2000-11-08 | Mutagenic maintenance method for standard female line of castor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1352876A CN1352876A (en) | 2002-06-12 |
| CN1163117C true CN1163117C (en) | 2004-08-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001318578A Expired - Lifetime CN1163117C (en) | 2000-11-08 | 2000-11-08 | Mutagenic maintenance method for standard female line of castor |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347093B (en) * | 2008-08-28 | 2012-10-17 | 吉林大学 | Method for quickly cultivating thermosensitive type recessive nueleus sterility isofemale line castor and use |
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- 2000-11-08 CN CNB001318578A patent/CN1163117C/en not_active Expired - Lifetime
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Owner name: TONGLIAO CITY TONGKE SEED CO.,LTD. Free format text: FORMER OWNER: TONGLIAO CITY AGRICULTURE SCIENCE INSTITUTE Effective date: 20060901 |
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Effective date of registration: 20060901 Address after: 028000 the Inner Mongolia Autonomous Region Tongliao Qian Jia Dian Zhen Dong Institute of Agricultural Sciences Patentee after: Tongliao Tongke seed industry limited liability company Address before: 025000 Zhelimumeng of the Inner Mongolia Autonomous Region Agricultural Science Research Institute Patentee before: Tongliao City Agriculture Science Institute |
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