CN1167329C

CN1167329C - Decontaminating and purity maintaining technology for three-line hybrid rice

Info

Publication number: CN1167329C
Application number: CNB001144928A
Authority: CN
Inventors: 张集文; 武晓智
Original assignee: Individual
Current assignee: Individual
Priority date: 2000-04-18
Filing date: 2000-04-18
Publication date: 2004-09-22
Anticipated expiration: 2020-04-18
Also published as: CN1318288A

Abstract

The present invention relates to a method for eliminating the mixed seedlings of a female parent as the sterile line of hybrid rice by a three-line method, and the seedlings of the maintenance line of the hybrid rice by using the herbicide of bentazone. A maintenance line and a nucleo-cytoplasmic interaction male sterility line which both have a lethal homozygous recessive character sensitive to the bentazone are selected for crossbreeding to produce the seeds of the sterile line, and the seeds of the hybrid rice is prepared from the produced sterile line and a restoring line without the recessive character; after the seeds of the hybrid rice are seeded, (0.001 to 1.0)% bentazone solution is sprayed. The method is used in the seed production of the hybrid rice by the three-line method or the fast identification of the purity of the seeds of hybridization.

Description

Pure method is protected in the series of three-series hybrid rice removal of impurities

The invention belongs to a kind of production method that improves hybrid rice purity, especially by chemicals treatment remove sneak into paddy rice cross breeding F1 in generation maternal male sterile line and the impurity removing technology of maintenance line seedling.

The Three-line Hybrid paddy rice utilizes the paddy rice cytoplasmic-nuclear male sterility to carry out seed production.The seed that is used for the maternal male sterile line of hybrid seeding is to obtain behind the outbreeding by male sterile line and maintenance line, and maintenance line then passes through self propagated.But because male sterile line male sterile is not thorough or mechanical admixture, be used for the hybrid rice seeds that the land for growing field crops produces, usually be mixed with the seed of male sterile line and maintenance line.This specific admixture is the main cause that hybrid rice seeds mixes, and causes production of hybrid seeds failure or production loss when serious.

In order to reject the male sterile line and the maintenance line seed of sneaking in the hybrid seed, it is carried out the proterties mark is a kind of otherwise effective technique.Known labeling method has form tracer method and chemical labeling method.Chinese patent publication number CN1217870A discloses a kind of method of removing maternal selfing rice shoot with the herbicides for use in paddy Bentazon in two line method and chemical emasculation method hybrid rice rice shoot.Data according to the document, selection has the mutant 8077 of the recessive character that can be caused death by Bentazon for maternal, with the recovery that does not have described recessive character be the preparing hybrid kind, this crossbreed after planting, its seedling is used Bentazon solution, and the female parent self-cross seedling that isozygotys will be killed, and real hybridization seedling is owing to be the heterozygous genes type, growth normally and not is injured, thereby has reached the purpose of removing maternal selfing rice shoot.The document does not relate to by the removing of the three line method male sterile line of outbreeding and maintenance line thereof protects pure problem.

The purpose of this invention is to provide and a kind ofly remove the technology of Three-line Hybrid paddy rice F1 for the seedling of maternal male sterile line of sneaking in the seed and maintenance line thereof by chemicals treatment.

Method of the present invention is achieved in that

A kind of method of removing the seedling of the maternal male sterile line of sneaking in the Three-line Hybrid paddy rice seedling and maintenance line thereof with the weed killer herbicide Bentazon, selection has the maintenance line of the recessive character of isozygotying of Bentazon sensitive lethality and has equally produces male-sterile seed to the cytoplasmic nuclear male sterile line outbreeding of the recessive character of isozygotying of Bentazon sensitive lethality, is the preparing hybrid rice with the male sterile line of being produced with the recovery that does not have described recessive character, after planting sprays the Bentazon solution of 0.001-1.0% at hybrid rice seed.

Above-mentioned method is used in the production of hybrid seeds of Three-line Hybrid paddy rice or hybrid seed purity Rapid identification.

Advantage of the present invention and good effect are as follows:

Can guarantee the field purity of Three-line Hybrid paddy rice.Studies have shown that impurity of seeds is the main cause of breeding of hybrid rice failure or production loss.The paddy rice cross breeding seed mixes every increase one percentage point, and output per hectare is with 75 kilograms of the underproduction.The present invention is for guaranteeing the production purity of hybrid rice, and the loss of avoiding seed production and land for growing field crops to produce provides a kind of reliable technique.Because the main source that the Three-line Hybrid rice paddy seed mixes is the seed of male sterile line and maintenance line, so the present invention also provides a kind of very simple and effective means for the purity check of Three-line Hybrid rice paddy seed.

Embodiments of the invention are;

II-32A is a good cytoplasmic nuclear male sterile line, and its maintenance is II-32B.The maternal male-sterile seed that is used for hybrid seeding is produced with II-32A * II-32B outbreeding.Adopt disclosed mutant 8077 hybridization among II-32B and the CN1217870A, and be that recurrent parent is repeatedly backcrossed, breed and have the isozygoty MII-32B of recessive character of Bentazon sensitive lethality with II-32B.Be that recurrent parent and II-32A backcross again with MII-32B, breed and have the isozygoty MII-32A of recessive character of Bentazon sensitive lethality.With MII-32A * MII-32B outbreeding, just can obtain to be used for the maternal male-sterile seed of hybrid seeding.With this maternal male-sterile seed and the recovery that do not have a Bentazon sensitive lethality recessive character is 838 preparing hybrid kinds, has just obtained to have excellent 838 hybrid seeds of MII of removal of impurities mark.Before this excellent 838 hybrid seeds of MII with removal of impurities mark were used for the land for growing field crops and produce, at first can take a sample after planting sprayed Bentazon solution in seedling stage, checks the seed that wherein whether is mixed with maternal male sterile line and maintenance line thereof.This has just reached the purpose of checking seed purity.Mix if seed has, then land for growing field crops can spray Bentazon solution seedling stage, removes the maternal male sterile line that mixes and the seedling of maintenance line thereof, thereby has avoided causing the loss of seed and production.

Claims

1 one kinds of methods with the seedling of maternal male sterile line of sneaking in the weed killer herbicide Bentazon removing Three-line Hybrid paddy rice seedling and maintenance line thereof, it is characterized in that selecting having to the maintenance line of the recessive character of isozygotying of Bentazon sensitive lethality and have equally the cytoplasmic nuclear male sterile line outbreeding of the recessive character of isozygotying of Bentazon sensitive lethality is produced male-sterile seed, is the preparing hybrid rice with the male sterile line of being produced with the recovery that does not have described recessive character, after planting sprays the Bentazon solution of 0.001-1.0% at hybrid rice seed.

2 methods according to claim 1 is characterized in that this method uses in the production of hybrid seeds of Three-line Hybrid paddy rice or hybrid seed purity Rapid identification.